Horiba Scientific DeltaMyc J810018 User manual

DeltaMyc
Fluorescence Lifetime
Imaging Microscope System
Operation Manual
Part number J810018

DeltaMyc Operation Manual J810018 (7 Oct 2014)
i
DeltaMyc™
Microscope Fluorescence
Lifetime Mapping System
Operation Manual
http://www.HORIBA.com

DeltaMyc Operation Manual J810018 (7 Oct 2014)
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Copyright © 2013, 2014 by HORIBA Instruments Incorporated. All rights reserved. No
part of this work may be reproduced, stored, in a retrieval system, or transmitted in any
form by any means, including electronic or mechanical, photocopying and recording,
without prior written permission from HORIBA Instruments Incorporated. Requests for
permission should be requested in writing. Windows®is a trademark of Microsoft Cor-
poration.
Information in this manual is subject to change without notice, and does not represent a
commitment on the part of the vendor.
October 2014
Part Number J810018

DeltaMyc Operation Manual J810018 (7 Oct 2014)
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Table of Contents
0: Introduction ........................................................................................... 0-1
About the DeltaMyc..........................................................................................................................0-1
Chapter overview .............................................................................................................................0-2
Disclaimer.........................................................................................................................................0-3
Safety summary ...............................................................................................................................0-5
Risks of ultraviolet exposure ............................................................................................................0-7
Additional risks of intense light sources...........................................................................................0-9
Laser light.......................................................................................................................................0-11
CE compliance statement..............................................................................................................0-12
1: Anatomy of the DeltaMyc ............................................................................ 1-1
Introduction.......................................................................................................................................1-1
Block diagram...................................................................................................................................1-2
DeltaMyc-Inverted............................................................................................................................1-3
DeltaMyc-Upright .............................................................................................................................1-4
2: Requirements & Installation ........................................................................ 2-1
Safety-training requirements............................................................................................................2-1
Surface requirements.......................................................................................................................2-2
Environmental requirements............................................................................................................2-3
Electrical requirements.....................................................................................................................2-4
Unpacking and Installation...............................................................................................................2-5
3: System Operation...................................................................................... 3-1
Introduction.......................................................................................................................................3-1
Controlling the hardware..................................................................................................................3-3
Single-point measurement.............................................................................................................3-11
Lifetime mapping............................................................................................................................3-16
Data-analysis using the DAS6 Foundation-level package.............................................................3-25
4: Maintenance & Troubleshooting .................................................................... 4-1
Maintenance.....................................................................................................................................4-1
Troubleshooting ...............................................................................................................................4-2
Further assistance… ........................................................................................................................4-6
5: USB Port Configuration ............................................................................... 5-1
6: Technical Specifications ............................................................................ 6-1
Introduction.......................................................................................................................................6-1
Microscope.......................................................................................................................................6-1
Sample compartment.......................................................................................................................6-1
Excitation sources............................................................................................................................6-1
Motorized stage................................................................................................................................6-1
TCSPC electronics...........................................................................................................................6-2
Detector............................................................................................................................................6-2
Filters................................................................................................................................................6-2
Software...........................................................................................................................................6-2
Dimensions.......................................................................................................................................6-2
Electrical...........................................................................................................................................6-2
Environment.....................................................................................................................................6-3
Minimum host-computer requirements ............................................................................................6-3
7: Index .................................................................................................... 7-1

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DeltaMyc Operation Manual J810018 (7 Oct 2014) Introduction
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Note: Keep this and the other reference manuals near the system.
0: Introduction
About the DeltaMyc
The DeltaMyc is a time-correlated single-photon counting (TCSPC) fluorescence life-
time system designed for the study of microscopic samples. It is the ideal system to
measure lifetimes ranging from 100 ps to 10 µs, on micron-scale features. Its confocal
and imaging capabilities makes it the ultimate tool for fluorescence microscopy inves-
tigation, with high spatial resolution and high timing sensitivity.
The system consists of the following components:
Microscope with motorized x,y stage and CCD camera
Confocal pinhole adapter
DeltaHub TCSPC timing electronics
Direct-coupled light source from the DeltaDiode series
DeltaDiode controller
PPD detector (near-IR, up to 900 nm, options available)
host computer with pre-installed data-acquisition cards and software: DataStation
measurement and control software; DAS decay-analysis software (Foundation-level
package)
The timing electronics are controlled through the intuitive DataStation measurement
control software application. Decay data measured using DataStation can be analyzed
using the DAS decay-analysis software package.
This manual explains how to operate and maintain a DeltaMyc microscope. The manu-
al also describes measurements and tests essential to obtain accurate data. Please refer
to the DataStation and DAS operation manuals for more information about how to use
the software. An in-depth description of the hardware items can also be found in their
respective manuals; we advise you to familiarize yourself with them. This operation
manual is primarily concerned with the installation, and providing an overview of the
DeltaMyc system operation, with measurement examples.

DeltaMyc Operation Manual J810018 (7 Oct 2014) Introduction
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Chapter overview
0: Introduction
Safety information
1: Anatomy of the DeltaMyc
Overall interconnection of the various parts of the DeltaMyc
2: Requirements & Installation
Power and environmental requirements; select the best spot
for the instrument and set-up.
3: System Operation
Operation of the fluorometer system, and calibration in-
structions.
4: Maintenance & Trouble-
shooting
Routine cleaning, and what to do should there be a problem.
5: USB Port Configuration
How the USB ports are configured.
6: Technical Specification
7: Index

DeltaMyc Operation Manual J810018 (7 Oct 2014) Introduction
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Disclaimer
By setting up or starting to use any HORIBA Instruments Incorporated product, you are
accepting the following terms:
You are responsible for understanding the information contained in this document. You
should not rely on this information as absolute or all-encompassing; there may be local
issues (in your environment) not addressed in this document that you may need to ad-
dress, and there may be issues or procedures discussed that may not apply to your situa-
tion.
If you do not follow the instructions or procedures contained in this document, you are
responsible for yourself and your actions and all resulting consequences. If you rely on
the information contained in this document, you are responsible for:
Adhering to safety procedures
Following all precautions
Referring to additional safety documentation, such as Material Safety Data Sheets
(MSDS), when advised
As a condition of purchase, you agree to use safe operating procedures in the use of all
products supplied by HORIBA Instruments Incorporated, including those specified in
the MSDS provided with any chemicals and all warning and cautionary notices, and to
use all safety devices and guards when operating equipment. You agree to indemnify
and hold HORIBA Instruments Incorporated harmless from any liability or obligation
arising from your use or misuse of any such products, including, without limitation, to
persons injured directly or indirectly in connection with your use or operation of the
products. The foregoing indemnification shall in no event be deemed to have expanded
HORIBA Instruments Incorporated’s liability for the products.
HORIBA Instruments Incorporated products are not intended for any general cosmetic,
drug, food, or household application, but may be used for analytical measurements or
research in these fields. A condition of HORIBA Instruments Incorporated’s ac-
ceptance of a purchase order is that only qualified individuals, trained and familiar with
procedures suitable for the products ordered, will handle them. Training and mainte-
nance procedures may be purchased from HORIBA Instruments Incorporated at an ad-
ditional cost. HORIBA Instruments Incorporated cannot be held responsible for actions
your employer or contractor may take without proper training.
Due to HORIBA Instruments Incorporated’s efforts to continuously improve our prod-
ucts, all specifications, dimensions, internal workings, and operating procedures are
subject to change without notice. All specifications and measurements are approximate,
based on a standard configuration; results may vary with the application and environ-
ment. Any software manufactured by HORIBA Instruments Incorporated is also under
constant development and subject to change without notice.
Any warranties and remedies with respect to our products are limited to those provided
in writing as to a particular product. In no event shall HORIBA Instruments Incorpo-

DeltaMyc Operation Manual J810018 (7 Oct 2014) Introduction
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rated be held liable for any special, incidental, indirect or consequential damages of any
kind, or any damages whatsoever resulting from loss of use, loss of data, or loss of
profits, arising out of or in connection with our products or the use or possession there-
of. HORIBA Instruments Incorporated is also in no event liable for damages on any
theory of liability arising out of, or in connection with, the use or performance of our
hardware or software, regardless of whether you have been advised of the possibility of
damage.