illumina iSeq 100 Parts list manual

iSeq 100
Sequencing System Guide
Document # 1000000036024 v05
March 2019
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For Research Use Only. Not for use in diagnostic procedures.

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Document # 1000000036024 v05
For Research Use Only. Not for use in diagnostic procedures.
ii
iSeq 100 Sequencing System Guide

Revision History
Document Date Description of Change
Document #
1000000036024 v05
March
2019
Updated software descriptions to iSeq Control Software v1.4:
• Updated instructions on configuring system settings, including moving
and renaming some user interface elements.
• Added descriptions of %Clusters PF and %Occupancy metrics, which
appear on the Sequencing screen.
• Allowed mapped network drive locations for the sample sheets and
output folders.
• Indicated that the software automatically renames sample sheets to
SampleSheet.csv.
Added links to the following pages:
• The iSeq 100 System Sample Sheet Template for Manual Mode.
• The bcl2fast Conversion Software support pages.
Added volumes of 1 nM 100% PhiX and AmpliSeq Library PLUSfor
Illumina libraries to prepare.
Added instruction to move the reference genomes repository for Local
Run Manager toa location other than drive C when restoring to system
factory settings.
Increased the maximum recommended cycles for Index Read 1 and
Index Read 2 to 10 cycles each.
Increased the number of cycles the cartridge supports to 322.
Referenced the Cluster Density Optimization Guide (document #
1000000071511) for detailed information on optimizing loading
concentration.
Clarified that a cartridge being thawed in a water bath must be stored at
-25°C to -15°C for at least one day before thawing.
Corrected AmpliSeq for Illumina Library PLUSto AmpliSeq Library PLUS
for Illumina.
Document #
1000000036024 v04
October
2018
Added recommended loading concentrations and dilution instructions for
Nextera DNAFlex for Enrichment, TruSeq DNA Nano, and TruSeq DNA
PCR-Free libraries.
Added information on using a normalization method that does not result
in single-stranded libraries.
Added descriptions of the two run modes, Local Run Manager and
Manual.
Added a 5% PhiX spike-in option and defined the purpose of each spike-
in percentage.
Added the following steps:
• Switching to the sbsadmin operating system account when installing
the control software, analysis modules, and other software.
• Power cycling the instrument when restoring to factory settings.
Referenced Illumina Adapter Sequences (document # 1000000002694)
to determine Index 2 (i5)orientations for a sample sheet.
Clarified the following points:
• Cartridges must be used immediately after thawing.
• The loading concentrations listed for Nextera DNAFlex and Nextera
Flex for Enrichment libraries are not applicable to other Nextera library
types.
• SureCell WTA3′is not a compatible library.
Document # 1000000036024 v05
For Research Use Only. Not for use in diagnostic procedures.
iii
iSeq 100 Sequencing System Guide

Document Date Description of Change
Document #
1000000036024 v03
August
2018
Updated software descriptions to iSeq Control Software v1.3:
• Added configuration instructions for Universal Copy Service.
• Renamed the Network Configuration tab to Network Access.
• Added instructions on opening Local Run Manager from the control
software.
Updated the default output folder location to D:\SequencingRuns.
Added instructions on connecting the system to a proxy server.
Added requirement to specify a UNCpath for output folder and sample
sheet locations on the network.
Indicated the unique requirements for configuring an output folder
location on an internal drive, external drive, or network location.
Made instructions on creating a sample sheet for Manual mode the first
step of run setup.
Corrected instructions on using the system suite installation wizard.
Corrected the description of output thumbnail files.
Document #
1000000036024 v02
June
2018
Updated tubes used for diluting libraries to Fisher Scientific catalog #
14-222-158 or equivalent low-bind tubes.
Added a section describing the regional availability of Advanced
Exchange.
Clarified that libraries diluted to the loading concentration must be
sequenced that day.
Clarified that the reagent cartridge must be removed from the box for
thawing.
Document #
1000000036024 v01
May
2018
Updated software descriptions to iSeq Control Software v1.2:
• Added option to browse to a downloaded software installer from the
control software.
• Added instructions for saving thumbnails.
• Moved network settings to the Network Configuration tab.
• Increased maximum uses of reusable test components to 36 and noted
that the remaining number of uses appear onscreen.
Updated Local Run Manager information:
• Added steps for opening Local Run Manager and setting up the run.
• Added RNAAmplicon as a preinstalled analysis module, and
DNAEnrichment and Resequencing as other supported modules.
• Updated documentation references to Local Run Manager Software
Guide (document #1000000002702) .
Updated cartridge thawing instructions:
• Added a room-temperature thawing option.
• Provided more detailed water bath instructions, including storing before
thawing.
Updated instructions on preparing libraries for sequencing:
• Updated the Nextera DNAFlex loading concentration to 200 pM.
• Added starting loading concentration for unlisted library types.
• Added information on the %Occupied metric.
• Increased volume of 1 nM PhiX for a spike-in to 50 µl.
Updated Illumina catalog numbers for:
• iSeq 100 Spare Drip Tray Pad to 20023927.
• iSeq 100 Spare Air Filter to 20023928.
Updated pipette and pipette tip recommendations.
Document # 1000000036024 v05
For Research Use Only. Not for use in diagnostic procedures.
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iSeq 100 Sequencing System Guide

Document Date Description of Change
Document #
1000000036024 v01
May
2018
Added the following miscellaneous instructions:
• Performing validation runs.
• Creating a sample sheet when sequencing in Manual mode.
• Minimizing the control software to access other applications.
Added the following steps to the system check procedure:
• Unloading and storing reusable test components.
• Cleaning visible debris from the reusable test flow cell.
Reorganized the following content to improve continuity:
• Merged instructions for performing a PhiX-only run with the standard
sequencing instructions.
• Merged flow cell preparation instructions with library dilution
instructions.
• Consolidated PhiX spike-in instructions.
• Moved information on number of cycles in a read.
• Moved Real-Time Analysis and renamed to Sequencing Output .
Simplified the error messages workflow diagram.
Removed information on Tablet and Desktop modes. The operating
system runs in Desktop mode by default and Tablet mode is not
necessary.
Removed requirement to complete and return a decontamination
certificate for advanced exchange.
Corrected average run size to 2 GB.
Document #
1000000036024 v00
February
2018
Initial release.
Document # 1000000036024 v05
For Research Use Only. Not for use in diagnostic procedures.
v
iSeq 100 Sequencing System Guide

Table of Contents
Chapter 1 Overview 1
Introduction 1
Additional Resources 2
Instrument Components 3
iSeq 100 i1 Reagent 7
Chapter 2 Getting Started 11
First Time Setup 11
Minimize the Control Software 11
Run Settings 12
Instrument Customization 14
Network Setup 15
User-Supplied Consumables and Equipment 17
Chapter 3 Sequencing 19
Introduction 19
Thaw the Bagged Cartridge 20
Prepare the Flow Cell and Libraries 21
Load Consumables Into the Cartridge 23
Set Up a Sequencing Run (Local Run Manager) 24
Set Up a Sequencing Run (Manual Mode) 27
Chapter 4 Maintenance 31
Clear Hard Drive Space 31
Software Updates 31
Replace the Air Filter 32
Relocate the Instrument 34
Software Restriction Policies 35
Appendix A Sequencing Output 38
Real-Time Analysis Overview 38
Real-Time Analysis Workflow 40
Appendix B Troubleshooting 44
Error Message Resolution 44
Cancel a Started Run 45
Power Cycle the Instrument 45
Perform a System Check 46
Leak Mitigation 48
Restore to Factory Settings 50
Appendix C Advanced Exchange 52
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Exchanging the iSeq 100 System 52
Receive a Replacement System 52
Prepare the Original System for Return 52
Return the Original System 55
Index 59
Technical Assistance 65
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iSeq 100 Sequencing System Guide

Chapter 1 Overview
Introduction 1
Additional Resources 2
Instrument Components 3
iSeq 100 i1 Reagent 7
Introduction
The Illumina®iSeq™ 100 Sequencing System provides a targeted approach to next-generation sequencing
(NGS). This applications-focused system packages Illumina sequencing technology into a cost-effective
desktop instrument.
Features
uAccessibility and reliability—The iSeq 100 System has a small footprint and is easy to install and use.
Fluidics and imaging components are built into the consumable, simplifying instrument maintenance.
uSingle-step consumable loading—A single-use cartridge is prefilled with all reagents required for a run.
Library and a sensor-equipped flow cell load directly into the cartridge, which is then loaded onto the
instrument. Integrated identification enables accurate tracking.
uiSeq 100 System software—A suite of integrated software controls instrument operations, processes
images, and generates base calls. This suite features on-instrument data analysis and data transfer tools
for external analysis.
uOn-instrument analysis—Local Run Manager inputs sample information and then analyzes run data
using the analysis module specified for the run. The software includes a suite of analysis modules.
uCloud-based analysis—The sequencing workflow is integrated with BaseSpace Sequence Hub, the
Illumina cloud computing environment for run monitoring, data analysis, storage, and collaboration.
Output files are streamed in real time to BaseSpace Sequence Hub for analysis.
Sample to Analysis
The following diagram illustrates the complete sequencing workflow, from experimental design to analyzing
data. Tools and documentation are included with each step. This guide covers the sequence libraries step.
For other documentation, visit support.illumina.com.
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Figure 1 Sample to Analysis Workflow
Additional Resources
The iSeq 100 Sequencing System support pages on the Illumina website provide additional system
resources. These resources include software, training, compatible products, and the following
documentation. Always check support pages for the latest versions.
Resource Description
Custom Protocol Selector A tool for generating end-to-end instructions tailored to your library prep method, run
parameters, and analysis method, with options to refine the level of detail.
iSeq 100 Sequencing System
Setup Poster (document #
1000000035963)
Provides instructions for instrument installation and initiating first time setup.
iSeq 100 Sequencing System Site
Prep Guide (document #
1000000035337)
Provides specifications for laboratory space, electrical requirements, and environmental
and network considerations.
iSeq 100 Sequencing System
Safety and Compliance Guide
(document # 1000000035336)
Provides information about operational safety considerations, compliance statements,
and instrument labeling.
RFID Reader Compliance Guide
(document # 1000000002699)
Provides information about the RFID reader in the instrument, including compliance
certifications and safety considerations.
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iSeq 100 Sequencing System Guide

Instrument Components
iSeq 100 Sequencing System comprises a power button, monitor, status bar, consumables compartment,
and drip tray.
Figure 2 External System Components
APower button—Controls instrument power and indicates whether the system is on (glows), off (dark), or off
but with ACpower (pulses).
BTouch-screen monitor—Enables on-instrument configuration and setup using the control software interface.
CStatus bar—Indicates system status as ready to sequence (green), processing (blue), or needs attention
(orange).
DConsumables compartment—Contains consumables during a run.
EDrip tray door—Provides access to the drip tray, which catches leaked fluids.
Power and Auxiliary Connections
You can move the instrument to access USBports and other rear panel components.
The back of the instrument has the switch and inlet that controls power to the instrument, and an Ethernet
port for an optional Ethernet connection. Two USBports provide the option to connect a mouse and
keyboard, or to upload and download data using a portable device.
NOTE
Connecting the system to a keyboard and mouse overrides the onscreen keyboard.
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iSeq 100 Sequencing System Guide

Figure 3 Rear Panel Components
AEthernet port—Optional Ethernet cable connection.
BUSBports—Two ports for connecting auxiliary components.
CToggle switch—Turns instrument power on and off.
DAC power inlet—Power cord connection.
Consumables Compartment
The consumables compartment contains the cartridge for a sequencing run.
Figure 4 Loaded Consumables Compartment
ACartridge—Contains the flow cell, library, and reagents, and collects used reagents during the run.
BTray—Holds the cartridge during sequencing.
CDoor—Opens to a 60 degree angle to provide access to the consumables compartment.
The software opens and closes the compartment door and positions the cartridge for imaging. The door
opens down from hinges toward the base of the instrument. Do not place objects on the open door, which is
not designed for use as a shelf.
Reusable Test Flow Cell and Cartridge
The instrument ships with the iSeq 100 Reusable Test Flow Cell and iSeq 100 Reusable Test Cartridge to use
for system checks.
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iSeq 100 Sequencing System Guide

uStore in the original packaging at room temperature.
uReuse up to 36 times or for 5 years from the date of manufacture, whichever comes first.
uDuring a system check, the software displays the remaining number of uses.
uReplace expired reusable test components with the iSeq 100 System Test Kit.
Figure 5 Reusable Test Components
AReusable test flow cell
BReusable test cartridge
The reusable test components look similar to the sequencing components provided in iSeq 100 i1 Reagent,
and the loading orientations are the same. However, the test cartridge does not have a library reservoir and
neither test component has the chemistry needed for a run.
System Software
The system software suite includes integrated applications that perform sequencing runs and on-instrument
analysis.
uiSeq Control Software—Controls instrument operations and provides an interface for configuring the
system, setting up a sequencing run, and monitoring run statistics as sequencing progresses.
uLocal Run Manager—Defines run parameters and the analysis method before sequencing. After
sequencing, on-instrument data analysis begins automatically.
uThe system is shipped with the DNAAmplicon, RNAAmplicon, and Generate FASTQ analysis
modules installed.
uThe system also supports the DNAEnrichment and Resequencing analysis modules, which are
available on the Local Run Manager support pages.
uFor more information on Local Run Manager and the analysis modules, see the Local Run Manager
Software Guide (document # 1000000002702) .
uReal-Time Analysis (RTA2)—Performs image analysis and base calling during the run. For more
information, see Sequencing Output on page 38.
uUniversal Copy Service—Copies sequencing output files from the run folder to BaseSpace Sequence
Hub (if applicable) and the output folder, where you can access them.
Real-Time Analysis and Universal Copy Service run background processes only. Local Run Manager and the
control software can require user input.
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iSeq 100 Sequencing System Guide

System Information
The control software menu has an About section where you can view Illumina contact information and the
following system information:
uSerial number
uComputer name and IPaddress
uRecipe fragment version
uRun count
Notifications and Alerts
An icon appears next to the instrument name to indicate notifications. Select the icon to view a list of the
notifications, which include warnings and errors.
uWarnings require attention, but do not stop a run or require action other than acknowledgment.
uErrors require action before starting or proceeding with a run.
A panel on the left side of run setup screens displays alerts specific to cartridge loading and pre-run checks.
Figure 6 Onscreen Locations
ARun setup alerts
BOther notifications
Process Management
The Process Management screen displays hard drive (drive D) space and run status, identifying each run by
name, ID, and date. The screen is automatically refreshed every three minutes.
The Status column indicates whether a run is progressing or complete, based on the processing of BCLfiles.
For each run, Process Management also displays the status ofbackground processes Universal Copy
Service, BaseSpace Sequence Hub, and Local Run Manager.
Nonapplicable processes do not appear on the screen. For example, if a run is not connected to BaseSpace
Sequence Hub, Process Management does not display the BaseSpace status for that run.
uTo troubleshoot status issues, see Process Management Status on page 44.
uTo delete runs and clear space, see Clear Hard Drive Space on page 31.
Status of Universal Copy Service
Universal Copy Serviceshows the status of files being copied to the output folder:
uIn Progress—Universal Copy Service is copying files to the output folder.
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iSeq 100 Sequencing System Guide

uComplete—Universal Copy Services successfully copied all files to the output folder.
Status of BaseSpace Sequence Hub
BaseSpace Sequence Hub shows the upload status:
uIn Progress—The control software is uploading files to BaseSpace Sequence Hub.
uComplete—All files are successfully uploaded to BaseSpace Sequence Hub.
Status of Local Run Manager
Local Run Manager shows the analysis status in control software:
uNot Started—The analysis is queued to start or Local Run Manager is waiting for Real-Time Analysis to
finish.
uIn Progress—Local Run Manager is analyzing files. Check the Local Run Manager software for a more
detailed status.
uStopped—The analysis has stopped, but is incomplete.
uComplete—Local Run Manager has successfully completed the analysis.
For more information on analysis status, check the Local Run Manager software.
iSeq 100 i1 Reagent
Performing a run on the iSeq 100 System requires one single-use iSeq 100 i1 Reagent kit. The kit is available
in one size (300-cycle)and two bundles:
uSingle—Includes consumables for one run.
uFour-pack—Includes consumables for four runs.
Contents and Storage
The iSeq 100 i1 Reagent kit provides the cartridge and flow cell for sequencing. When you receive the kit:
uDo not open the white foil packaging until instructed to do so during the cartridge thawing and flow cell
preparation procedures. The cartridge is thawed in the bag.
uPromptly store components at the indicated temperatures to ensure proper performance.
uStore cartridges so that the package labels face up.
uStore cartridges for at least one day before thawing in a water bath.
Bundle Consumable Quantity Storage Temperature
Single Cartridge 1 -25°C to -15°C
Flow cell 1 2°C to 8°C*
Four-pack Cartridge 4 -25°C to -15°C
Flow cell 4 2°C to 8°C*
Table 1 Kit Components
*Shipped at room temperature.
Both consumables have identifiers for tracking and ensuring compatibility. The cartridge uses
radio-frequency identification (RFID). The flow cell uses an electrical interface: electrically erasable
programmable read-only memory (EEPROM).
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iSeq 100 Sequencing System Guide

Flow Cell
The iSeq 100 i1 Flow Cell is a patterned, single-lane flow cell built over a complementary metal oxide
semiconductor (CMOS)optical sensor. A plastic cartridge encases the glass-based flow cell. Raised grip
points on the plastic ensure safe handling.
AGrip points
BCMOSsensor (top)
CImaging area
DGasket (one of two)
ECMOS sensor (bottom)
FElectrical interface
Millions of nanowells cover the surface of the flow cell. Clusters are generated in the nanowells, from which the
sequencing reaction is then performed. The ordered arrangement of the nanowells increases output reads
and data. During sequencing, the CMOS sensor captures images for analysis.
Cartridge
The iSeq 100 i1 Cartridge is prefilled with clustering, sequencing, paired-end, and indexing reagents. A
foil-sealed reservoir is reserved for libraries, and a slot on the front is reserved for the flow cell. Illuminator light
reaches the flow cell through an access window on top of the cartridge.
AAccess window
BFlow cell slot
CLibrary reservoir
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iSeq 100 Sequencing System Guide

The cartridge holds all consumables for a run:reagents, library, and flow cell. The library and flow cell are
loaded into the thawed cartridge, which is then loaded onto the instrument. After the run begins, reagents
and library are automatically transferred from the cartridge to the flow cell.
An underside reservoir collects used reagents. The cartridge also contains pumps, valves, and all other
fluidics for the system. Because the cartridge is discarded after a run, instrument washes are not necessary.
Supported Number of Cycles
The 300-cycle label on the cartridge indicates how many cycles are analyzed, not how many cycles are
performed. The flow cell is compatible with any number of cycles and any read type.
The cartridge provides sufficient reagent for up to 322 cycles of sequencing. The 322 cycles include 151
cycles each for Read 1 and Read 2, plus up to 10 cycles each for Index 1 and Index 2. For information on
how many cycles to sequence, see Recommended Number of Cycles on page 19.
Symbol Descriptions
The following table describes the symbols on the consumable or consumable packaging.
Symbol Description
The date the consumable expires. For best results, use the consumable before this date.
Indicates the manufacturer (Illumina).
The date when the consumable was manufactured.
The intended use is Research Use Only (RUO).
Indicates the part number so that the consumable can be identified.*
Indicates the batch code to identify the batch or lot that the consumable was
manufactured in.*
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Symbol Description
Indicates that caution is necessary.
Indicates a health hazard.
Storage temperature range in degrees Celsius. Store the consumable within the indicated
range.
* REF identifies the individual component, while LOTidentifies the lot or batch the component belongs to.
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iSeq 100 Sequencing System Guide

Chapter 2 Getting Started
First Time Setup 11
Minimize the Control Software 11
Run Settings 12
Instrument Customization 14
Network Setup 15
User-Supplied Consumables and Equipment 17
First Time Setup
The first time the system is turned on, the control software is launched with a series of screens to guide you
through first time setup. First time setup includes performing a system check to confirm instrument
performance and configuring system settings.
If you want to modify system settings after first time setup, select the System Settings command in the control
software. The command opens the Settings, Network Access, and Customization tabs, where you can
access all control software settings and Windows network settings.
Operating System Accounts
The Windows operating system has two accounts: administrator (sbsadmin)and user (sbsuser).
The administrator account is intended for IT use, system updates, and installing the control software, Local
Run Manager analysis modules, and other software. Perform all other functions, including sequencing, from
the user account.
Password Requirements
The operating system requires a password change for both accounts (sbsadmin and sbsuser) at the first
logon. Update each password every 180 days, when prompted.
Policy Setting
Enforce password history Five passwords remembered
Lockout threshold Ten invalid logon attempts
Minimum password length Ten characters
Password must meet complexity requirements Disabled
Store passwords using reversible encryption Disabled
Table 2 Default Password Policies
Validation Runs
Optionally perform a validation run before sequencing experimental libraries for the first time. A validation run
sequences 100% PhiX, which functions as a control library, to confirm system operation. For instructions, see
Sequencing on page 19.
Minimize the Control Software
Minimize the control software to access other applications. For example, to browse to the output folder in File
Explorer or find a sample sheet.
1 Swipe up on the touch screen to open the Windows taskbar.
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2 Select the iSeq 100 System icon or another application.
The control software is minimized.
3[Optional] Attach a keyboard and mouse to the instrument to facilitate navigation and typing outside of
the control software.
4 To maximize the control software, swipe up and select iSeq 100 System.
Run Settings
Configure options for run setup, run monitoring, and data analysis on the Settings tab in System Settings. This
tab displays recommended express settings, which you can apply by selecting the express setup option.
Alternatively, select the manual setup option to customize the settings.
Choosing express settings applies the following settings and sends InterOp files, log files, instrument
performance data, and run data to BaseSpace Sequence Hub:
uIllumina Proactive Support—Facilitate troubleshooting and detect potential failures, allowing proactive
maintenance and maximizing instrument uptime. Turning on Illumina Proactive Support sends instrument
performance data (not sequencing data) to BaseSpace Sequence Hub. For more information, see the
Illumina Proactive Technical Note (document # 1000000052503) .
uLocal Run Manager—Use the Local Run Manager software to create runs and analyze run data for a
simple, streamlined workflow. Separate sample sheets and analysis applications are not needed.
uRemote Run Monitoring—Use BaseSpace Sequence Hub for remote run monitoring.
uRun Analysis, Collaboration, and Storage—Use BaseSpace Sequence Hub to store and analyze run data
and collaborate with colleagues.
NOTE
Local Run Manager automatically starts analysis when the run is complete. However, you can also analyze
data in BaseSpace Sequence Hub.
Apply Express Settings
Express setup replaces the current run settings with recommended run settings and localized settings for
BaseSpace Sequence Hub. These settings require an internet connection and a BaseSpace Sequence Hub
account. For account setup instructions, see the BaseSpace Sequence Hub Online Help (document #
1000000009008) .
1 On the control software menu, select System Settings.
2 On the Settings tab, select Use Express Settings.
3 In the Set Region list, select the geographic location where the system resides or the location closest to
where the system resides.
This setting ensures that data are stored in the appropriate location for BaseSpace Sequence Hub.
4 If you have an Enterprise subscription, in the Enter Private Domain field, enter the domain name
(URL)used for single sign-on to BaseSpace Sequence Hub.
For example: https://yourlab.basespace.illumina.com.
5 Select Next.
6 Review the settings. To modify a setting:
a Select Edit to open the setting.
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b Modify the setting as needed, and then select Next.
c Select Next to continue through any subsequent screens.
On the Settings Review screen, a green checkmark indicates enabled settings.
7 Select Save.
8 To close System Settings, select Exit.
Manually Configure Settings
Manual setup guides you through each screen on the Settings tab to configure run settings, which have the
following requirements:
uTurning on Illumina Proactive Support and BaseSpace Sequence Hub requires an internet connection.
BaseSpace Sequence Hub also requires an account. For account setup instructions, see the BaseSpace
Sequence Hub Online Help (document # 1000000009008) .
uUsing BaseSpace Sequence Hub for data analysis when the system is configured for Manual mode
requires a sample sheet. For details, see Sample Sheet Requirements on page 14.
1 On the control software menu, select System Settings.
2 Select Set Up Manually.
3 Choose whether to enable to the Illumina Proactive Support service:
uTo turn it on, select the Turn on Illumina Proactive Support checkbox.
uTo turn it off, deselect the Turn on Illumina Proactive Support checkbox.
The service sends instrument performance data, such as temperature and run time, to Illumina. These
data help Illumina detect potential failures and facilitate troubleshooting. Run data are not sent. For more
information, see the Illumina Proactive Technical Note (document # 1000000052503) .
4 Select Next.
5 Choose whether to connect runs to BaseSpace Sequence Hub:
uTo connect runs, select one of the following checkboxes:
uTurn on run monitoring from anywhere only—Use BaseSpace Sequence Hub for remote
monitoring.
uTurn on run analysis, collaboration, and storage also—Use BaseSpace Sequence Hub for remote
monitoring and analysis.
uTo disconnect runs, deselect the Turn on run monitoring from anywhere only and Turn on run
analysis, collaboration, and storage also checkboxes.
When connected, the control software sends InterOp and log files to BaseSpace Sequence Hub. The run
analysis, collaboration, and storage option also sends run data.
6 In the Set Region list, select the geographic location where the system resides or the location closest to
where the system resides.
This setting ensures that data are stored in the appropriate location for BaseSpace Sequence Hub.
7 If you have an Enterprise subscription, in the Enter Private Domain field, enter the domain name
(URL)used for single sign-on to BaseSpace Sequence Hub.
For example: https://yourlab.basespace.illumina.com.
8 Select Next.
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