iota Sciences isoHub User manual

2.1.0-3
isoHub user manual

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In August of 2005, the European Union (EU) implemented the EU WEEE Directive
2002/96/EC and later the WEEE Recast Directive 2012/19/EU requiring Producers of
electronic and electrical equipment (EEE) to manage and finance the collection,
reuse, recycling and to appropriately treat WEEE that the Producer places on the EU
market after August 13, 2005. The goal of this directive is to minimize the volume of
electrical and electronic waste disposal and to encourage re-use and recycling at
the end of life.
Products bearing the symbol of a strikethrough refuse bin must not be disposed of
along with unsorted refuse.
To dispose of your isoHub at the end of its useful life, please contact iotaSciences
for the correct procedure.
NOTE: This equipment has been tested and found to comply with the limits for a Class B digital
device, pursuant to part 15 of the FCC Rules. These limits are designed to provide reasonable
protection against harmful interference in a residential installation. This equipment generates,
uses and can radiate radio frequency energy and, if not installed and used in accordance with
the instructions, may cause harmful interference to radio communications. However, there is no
guarantee that interference will not occur in a particular installation. If this equipment does
cause harmful interference to radio or television reception, which can be determined by turning
the equipment off and on, the user is encouraged to try to correct the interference by one or
more of the following measures:
—Reorient or relocate the receiving antenna.
—Increase the separation between the equipment and receiver.
—Connect the equipment into an outlet on a circuit different from that to which the
receiver is connected.
—Consult iotaSciences for further help.
FCC CAUTION: Any changes or modifications to the isoCell other than those shown in the
manual, demonstrated during training, or directed by iotaSciences authorised personnel may
void compliance and the user’s authority to operate the isoCell.

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Contents
1 Introduction.........................................................................................................................................................................................................................................5
2 Safety instructions.......................................................................................................................................................................................................................5
3 Getting started.................................................................................................................................................................................................................................6
3.1 Familiarisation......................................................................................................................................................................................................................6
3.1.1 Accessories...............................................................................................................................................................................................................8
3.2 Installation................................................................................................................................................................................................................................8
3.2.1 Remove isoHub from packaging and place on a flat stable surface ................................................8
3.2.2 Attach the condenser portion..........................................................................................................................................................9
3.2.3 Insert phase contrast slider..................................................................................................................................................................9
3.2.4 Attach objectives............................................................................................................................................................................................10
3.2.5 Attach controller..............................................................................................................................................................................................10
3.2.6 Insert power cord............................................................................................................................................................................................ 11
3.2.7 Attach 60mm dish holder or well plate holder .......................................................................................................... 11
4 Using the isoHub........................................................................................................................................................................................................................... 11
4.1 Powering-on.........................................................................................................................................................................................................................11
4.2 Moving the stage..........................................................................................................................................................................................................12
4.3 Running programs........................................................................................................................................................................................................12
4.4 Chamber states................................................................................................................................................................................................................13
4.5 Looking around a chamber...............................................................................................................................................................................14
4.6 Moving to the next chamber.........................................................................................................................................................................15
4.7 Moving to the previous chamber.............................................................................................................................................................15
4.8 Changing chamber state......................................................................................................................................................................................15
4.9 Using the touchscreen to move between chambers and change states..............................................16
4.10 Program settings............................................................................................................................................................................................................16
4.10.1 Invert...............................................................................................................................................................................................................................16
4.10.2 Speed.............................................................................................................................................................................................................................16
4.10.3 Centre............................................................................................................................................................................................................................16
4.11 Sleep mode...........................................................................................................................................................................................................................16

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4.12 Powering-off....................................................................................................................................................................................................................... 17
5 Workflow...............................................................................................................................................................................................................................................17
5.1 Identify....................................................................................................................................................................................................................................... 17
5.2 Deactivate.............................................................................................................................................................................................................................. 17
5.3 Prep Detach..........................................................................................................................................................................................................................17
5.4 Prep Extract..........................................................................................................................................................................................................................18
5.4.1 Adhesion cells .....................................................................................................................................................................................................18
5.4.2 Suspension cells................................................................................................................................................................................................18
5.5 Prep Redetach...................................................................................................................................................................................................................18
6 Other programs.............................................................................................................................................................................................................................18
6.1 Data................................................................................................................................................................................................................................................18
6.2 Options.......................................................................................................................................................................................................................................19
6.3 Set time.....................................................................................................................................................................................................................................19
6.4 Data sync.................................................................................................................................................................................................................................19
6.4.1 Setting up data syncing........................................................................................................................................................................ 20
6.5 About ...........................................................................................................................................................................................................................................21
7 Bright field/phase contrast observation........................................................................................................................................................21
7.1 Changing between bright field and phase contrast observation....................................................................21
7.2 Adjusting the aperture iris diaphragm................................................................................................................................................21
7.3 Selecting the objective.........................................................................................................................................................................................22
7.4 Changing between visual light path and camera light path..................................................................................22
7.5 Focusing on the specimen................................................................................................................................................................................22
7.6 Adjusting the brightness.....................................................................................................................................................................................23
8 Advanced observation........................................................................................................................................................................................................23
8.1 Adjusting binocular position..........................................................................................................................................................................23
8.1.1 Adjusting the interpupillary distance....................................................................................................................................23
8.1.2 Adjusting the diopter............................................................................................................................................................................... 24
8.1.3 Using the eye shades............................................................................................................................................................................... 24
8.2 Adjusting tension of coarse focusing knob.................................................................................................................................25

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9 Recording with the camera...........................................................................................................................................................................................25
9.1 Changing between the visual light path and the camera light path..........................................................25
9.2 Focusing on the specimen................................................................................................................................................................................25
9.3 Acquiring the image.................................................................................................................................................................................................25
10 Maintenance .............................................................................................................................................................................................................................26
10.1 Cleaning ...................................................................................................................................................................................................................................26
11 Troubleshooting....................................................................................................................................................................................................................26
12 Disposal and recycling ..................................................................................................................................................................................................29
12.1 Disposal of isoHub......................................................................................................................................................................................................29
12.1.1 EU Waste Electrical and Electronic Equipment (WEEE) Directive.....................................................29
12.1.2 Users outside the European Union ..........................................................................................................................................30
13 Technical support................................................................................................................................................................................................................30
14 Additional information.................................................................................................................................................................................................30
14.1 Acknowledgements..................................................................................................................................................................................................30
14.2 Limited warranty............................................................................................................................................................................................................ 31
14.3 Limited liability................................................................................................................................................................................................................. 31
14.4 Medical use restrictions.......................................................................................................................................................................................32
14.5 Registered office...........................................................................................................................................................................................................32
14.6 Legal information.........................................................................................................................................................................................................32

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1Introduction
The isoHub works with the isoCell as part of the iotaSciences’ Cloning Platform to track colonies in
a GRID through the single-cell cloning workflow. The controller and automated stage make it easy
to review chambers in a GRID and tag chambers to include in the next step of the cloning workflow,
from identifying chambers containing single-cells (monoclonal chambers), to selecting colonies
ready to extract from the GRID.
The isoHub manages all of the user dish data in the system and automatically syncs the data
wirelessly with linked isoCells when needed to run programs.
2Safety instructions
Use the isoHub with care, and take the following precautions:
•Only use the isoHub once you have read and understood this manual. Ensure this manual
is accessible for all users, and that each potential user reads and understands it.
•If the isoHub is used in a manner not specified by the manufacturer, then the protection
provided by the isoHub may be impaired.
•Powering the equipment:
oUse only with the supplied XP-POWER VEC50US24 (24VDC, 50W) power supply
and power cord.
oConnect power supply to a suitable AC mains supply with voltage in range 90 –
260V; 50 –60Hz; minimum current 1A at 115V, 0.7A at 230V.
oThe AC mains socket shall act as the disconnect device and be readily accessible.
•Avoid subjecting the isoHub to impact and vibration.
•Keep the original packaging the isoHub came in, and use this when transporting the isoHub.
•Do not connect cables in ways other than those mentioned in this manual. Different
connections may cause damage and fire.
•Do not alter the isoHub in any manner, other than those shown in the manual,
demonstrated during training, or directed by iotaSciences.
•Only use the isoHub when it is set on top of a safe, flat and stable horizontal surface.
•If water or other liquid has been spilled near the isoHub, safety may be compromised.
Ensure that no spilled liquid has been in contact with or entered the isoHub.

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•If water or other liquid enters the isoHub, stop using it immediately and unplug the power
supply. Continued use may cause a fire. Contact iotaSciences for actions or precautions.
•If any foreign object enters the isoHub, safety may be compromised. Contact iotaSciences
for actions or precautions.
•Unplug the isoHub immediately if it smokes, or produces a strange odour or unusual noise.
Continued use may cause a fire. Contact iotaSciences for actions or precautions.
•Do not autoclave the isoHub.
•The isoHub is for indoor use only.
•The isoHub is designed to work in the following operating environment (operating outside
these limits may degrade performance and damage the isoHub):
oAmbient temperature: 16 –35°C (60 –95°F)
oHumidity: 40 –60%
oAltitude: less than 2000m
oPollution degree 2
•All services shall be performed by iotaSciences authorised personnel only.
•Do not look directly into the LED light source and be careful when looking down the
eyepiece that the LED isn't set too bright.
•When attaching a camera to the isoHub, ensure the isoHub remains stable.
•Use of the isoHub with other separately available items may cause abnormal operation and
may also damage the product. Contact iotaSciences for more information.
•External influences such as electrostatic discharge may cause this equipment to
malfunction. In this case, please disconnect the power and reconnect.
3Getting started
3.1 Familiarisation
Users should familiarise themselves with the key components of the isoHub prior to use:

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Figure 1: Front view showing key components of the isoHub.
Figure 2: Back view showing key components of the isoHub.

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3.1.1 Accessories
The isoHub is supplied with the following accessories:
Documents (also available to download from the iotaSciences customer portal):
•Quickstart user guide
•Training guide
Mains power cable, with appropriate mains plug for country of use
Power supply (24V XP-POWER VEC50US24 )
Condenser portion
Phase contrast slider
Controller
60mm dish holder
Well plate holder
Dust cover
Heated centre plate (optional)
3.2 Installation
3.2.1 Remove isoHub from packaging and place on a flat stable surface
When carrying the isoHub to a different
location, remove all cables and units in
advance.
Hold the lower area [a] of the binocular portion
above the touchscreen, and the lower part of
the column [b] or the hand grip [c] at the
bottom of the column, and install the isoHub at
the predefined location. Alternatively, slide the
isoHub off the table and lift from under the base of the isoHub.

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Do not manoeuvre by holding the eyepieces, light source, stage or the focusing knobs,
as this may cause damage to the isoHub.
Do not carry the isoHub with a specimen on the stage.
3.2.2 Attach the condenser portion
If using the isoHub with the fluorescence option, then attach the umbra shield to the
condenser portion before attaching the condenser portion to the isoHub –see the
isoHub fluorescence user manual available from the iotaSciences customer portal for
more details.
Loosen the condenser fixing knob [a] to the
extent that it does not fall off.
Hold the condenser diagonally, contact the
attaching portion on the top surface [b] of the
condenser to the round dovetail portion on
the bottom of the column and fit it.
Rotate the condenser and set the condenser
fixing knob [a] at the right side position
approximately. Then, tighten the condenser fixing knob [a] to secure it.
3.2.3 Insert phase contrast slider
Set the phase contrast slider [b] with the
surface where the texts are described facing
up, and insert it in the phase contrast slider
hole [a] of the condenser portion.
Align the empty hole in the light path for
bright field observation, or the pre-centred
phase contrast ring for phase contrast
observation.