Kendro HERAcell 240 User manual
Operating Instructions
HERAcell
®
240 CO
2
Incubator
with Decontamination Routine
Version: 03.2001 / 50065640
HERAcell®240 CO2Incubator Operating Instructions
- 2- Version: 03.2001 / 50065640
HERAcell®240 CO2Incubator Operating Instructions
Version: 03.2001 / 50065640 - 3-
Contents
1. Information regarding Safe Operation...............................................................................................................
6
1.1 Operator Information.....................................................................................................................................
6
1.2 User Information ...........................................................................................................................................
7
1.3 Explanation of Symbols ................................................................................................................................
8
1.4 General Safety Information.........................................................................................................................
10
1.5 Safety Information Concerning CO2............................................................................................................
12
2. Initial Inspection.................................................................................................................................................
13
2.1 Packaging ...................................................................................................................................................
13
2.2 Receipt Inspection ......................................................................................................................................
13
2.3 Parts supplied............................................................................................................................................. 1
4
3. Location Requirements.....................................................................................................................................
15
3.1 Ambient Conditions.....................................................................................................................................
15
3.2 Room Ventilation.........................................................................................................................................
15
3.3 Space Requirements ..................................................................................................................................
16
3.4 Moving.........................................................................................................................................................
17
3.5 Stacking ......................................................................................................................................................
18
3.6 Door Modifications......................................................................................................................................
18
3.7 Drawer.........................................................................................................................................................
18
4. Parts Identification.............................................................................................................................................
19
4.1 Front View...................................................................................................................................................
19
4.2 Rear View....................................................................................................................................................
20
5. System Description ...........................................................................................................................................
21
5.1 Sample Chamber Atmosphere ...................................................................................................................
21
5.2 ContraCon Decontamination Routine.............................................................................................................
21
5.3 Sensors.......................................................................................................................................................
22
5.4 Door Interlock Switch..................................................................................................................................
22
5.5 Supply Connections....................................................................................................................................
23
5.6 Sample Chamber Layout............................................................................................................................
24
6. Initial Setup.........................................................................................................................................................
26
6.1 Sample Chamber Preparation....................................................................................................................
26
6.2 Rack System Installation.............................................................................................................................
26
6.3 Gas Connection..........................................................................................................................................
29
6.4 Power Connection.......................................................................................................................................
30
6.5 RS 232 Interface Connection......................................................................................................................
31
6.6 Alarm Contact Connection..........................................................................................................................
33
6.7 Cleaning......................................................................................................................................................
35
7. Control, Displays, Indicators............................................................................................................................
36
7.1 Main Power Switch .....................................................................................................................................
36
7.2 Control Panel..............................................................................................................................................
36
7.3 Microprocessor Self-test.............................................................................................................................
37
7.4 Temperature Setpoint Specification............................................................................................................
38
7.5 CO2Setpoint Specification..........................................................................................................................
39
7.6 Setting the High/Low Humidity....................................................................................................................
39
7.7 auto-start Activation....................................................................................................................................
40
7.8 Error Codes.................................................................................................................................................
41
7.9 Error Code Overview ..................................................................................................................................
43
7.10 Resetting the Overtemperature Protector...................................................................................................
44
8. Operation...........................................................................................................................................................
45
8.1 Preparing the Unit.......................................................................................................................................
45
8.2 Beginning Operation...................................................................................................................................
45
9. Decommissioning..............................................................................................................................................
46
9.1 Taking the Unit Out of Service....................................................................................................................
46
HERAcell®240 CO2Incubator Operating Instructions
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Contents
10. Cleaning and Disinfection.................................................................................................................................
47
10.1 Preparation .................................................................................................................................................
47
10.2 Cleaning......................................................................................................................................................
49
10.3 Manual Disinfection.....................................................................................................................................
49
10.4 ContraCon Decontamination Routine.............................................................................................................
51
10.5 ContraCon Decontamination Routine Activation ........................................................................................
53
10.6 Interrupting the ContraCon Decontamination Routine................................................................................
53
11. Maintenance.......................................................................................................................................................
54
11.1 Inspections and Checks..............................................................................................................................
54
11.2 Maintenance Intervals.................................................................................................................................
54
11.3 Checking the Temperature.........................................................................................................................
55
11.4 Equalizing the Temperature........................................................................................................................
56
11.5 Checking the CO2Concentration................................................................................................................
57
11.6 Equalizing the CO2Concentration..............................................................................................................
58
11.7 Sterile Filter Replacement for the CO2Gas Supply....................................................................................
59
11.8 Unit Fuse Replacement ..............................................................................................................................
60
11.9 Door Seal Replacement..............................................................................................................................
60
12. Spare Parts and Accessories...........................................................................................................................
61
12.1 List of Spare Parts and Accessories ...........................................................................................................
61
13. Technical Specifications...................................................................................................................................
63
14. Materials Employed...........................................................................................................................................
65
Attachments
A Principles of Good Microbiology Techniques................................................................................................
66
B Unit Log ..............................................................................................................................................................
68
Addresses...................................................................................................................................................................
70
HERAcell®240 CO2Incubator Operating Instructions
Version: 03.2001 / 50065640 - 5-
Index of Figures
Fig. 1 Unit dimensions and minimum distances..................................................................................................
16
Fig. 2 Lift points ...................................................................................................................................................
17
Fig. 3 Stacking.....................................................................................................................................................
17
Fig. 4 Drawer installation.....................................................................................................................................
18
Fig. 5 Front view..................................................................................................................................................
19
Fig. 6 Rear view...................................................................................................................................................
20
Fig. 7 Metering cell sensors.................................................................................................................................
22
Fig. 8 Door interlock switch .................................................................................................................................
22
Fig. 9 Supply connections ...................................................................................................................................
23
Fig. 10 Sample chamber layout...........................................................................................................................
24
Fig. 11 Water reservoir........................................................................................................................................
25
Fig. 12 Rear unit wall openings...........................................................................................................................
25
Fig. 13 Support profile installation/removal .........................................................................................................
26
Fig. 14 Support bracket / insert tray installation..................................................................................................
27
Fig. 15 Center support installation.......................................................................................................................
28
Fig. 16 Gas supply line connection......................................................................................................................
29
Fig. 17 Power connection....................................................................................................................................
30
Fig. 18 RS 232 interface......................................................................................................................................
31
Fig. 19 Example of a connection diagram for an external alarm system ............................................................
33
Fig. 20 Switch the unit on/off...............................................................................................................................
36
Fig. 21 Control panel functions............................................................................................................................
36
Fig. 22 Protective screen/fan wheel disassembly ...............................................................................................
48
Fig. 23 Schematic diagram of the decontamination routine process ..................................................................
52
Fig. 24 Temperature equalization........................................................................................................................
55
Fig. 25 CO2equalization......................................................................................................................................
57
Fig. 26 Sterile filter replacement..........................................................................................................................
59
Fig. 27 Unit fuse replacement..............................................................................................................................
60
Fig. 28 Door seal replacement ............................................................................................................................
60
HERAcell®240 CO2Incubator Operating Instructions
- 6- Version: 03.2001 / 50065640
1. Information regarding Safe Operation
1.1
Operator Information
The HERAcell®240 CO2Incubator has been manu-
factured according to the highest standards and is
operationally safe. Nonetheless, this unit presents a
source of potential hazard, particularly when used by
individuals with inadequate instruction or if it is used in
an improper manner or for work for which it is not in-
tended.
•Using these operating instructions, the safety data
sheets, hygienic guidelines, and in compliance
with applicable technical regulations, the operator
must prepare written instructions in an under-
standable form for the users of this unit.
•These instructions are to be provided in the lan-
guage of the employees.
•Using these instructions, instruct the operating
and cleaning personnel in the functions, the ope-
ration, and the required cleaning tasks for this
unit.
•The content of these operating instructions is
subject to change at any time without notification.
•The German version of these operating instructi-
ons remains binding for all translations.
•Carefully store these operating instructions near
the unit so that operating personnel can refer to
them with regard to the safety information and im-
portant operating information.
For your own safety, please contact Kendro Laborato-
ry Products or your dealer should any questions arise
which are not adequately covered by these operating
instructions.
Germany
Address:
Kendro Laboratory Products GmbH
Heraeusstr. 12-14
D - 63450 Hanau, Germany
Deutschland
Telephone:
Sales + 49 (0) 1805-536376
Service + 49 (0) 1805-112110
Fax:
Sales / Service + 49 (0) 1805-112114
HERAcell®240 CO2Incubator Operating Instructions
Version: 03.2001 / 50065640 - 7-
1. Information regarding Safe Operation
Warranty:
Kendro Laboratory Products only warrants the safety
and operability of the HERAcell®240 CO2Incubator
under the condition that:
•The unit is employed solely for its intended purpo-
se and is operated and maintained in accordance
with the information in these operating instructi-
ons;
•No structural alterations are made to the unit;
•Only original spare parts and accessories appro-
ved by Kendro Laboratory Products are used;
•All inspection and maintenance tasks are perfor-
med at the specified intervals.
The warranty period begins at the time the unit is deli-
vered to the ordering party.
Trademarks:
Heraeus, HERAcell®are registered trademarks of
Kendro Laboratory Products GmbH. Any other trade-
marks cited in these operating instructions are the
sole property of the associated manufacturers.
Copyright©
These operating instructions are copyrighted. Reprin-
ting, photomechanical or digital processing or repro-
duction – including extracts – are therefore only
permitted with the written approval of Kendro Labora-
tory Products GmbH.
This does not apply to the reproduction for use solely
within the operator’s company.
1.2
User Information
It is important for you to read these operating instruc-
tions carefully before using the unit for the first time.
This will allow you to exploit all the advantages the
unit offers and to protect individuals, samples, and the
environment against possible harm:
•These operating instructions describe the HERA-
cell®240 CO2Incubator.
•The CO2Incubator may only be operated by
trained, authorized personnel.
•Maintenance work on the unit may only be per-
formed by the Kendro Laboratory Products Ser-
vice or trained, authorized agents.
HERAcell®240 CO2Incubator Operating Instructions
- 8- Version: 03.2001 / 50065640
1.3
Explanation of Symbols
Symbols Used in These Operating Instructions:
WARNING!
Failure to observe this instruction can result in severe
or fatal injury.
CAUTION!
Failure to observe this instruction can result in slight to
serious injury or property damage.
NOTE!
Indicates important information and application tips.
RECYCLING!
Valuable raw materials can be recovered.
Wear protective gloves!
Wear safety glasses!
Hazardous liquids!
Electrical shock hazard!
Hot surface!
Fire hazard!
1. Information regarding Safe Operation
Symbols in the Short Instructions:
Operator steps to be carried out on
the unit
Unit display status
Switch the unit on.
Switch the unit off.
Open the unit doors.
Leave the unit doors open for at
least 30 seconds.
HERAcell®240 CO2Incubator Operating Instructions
Version: 03.2001 / 50065640 - 9-
Symbols in the Short Instructions:
Close unit doors.
Remove samples and water reser-
voir from the unit.
Load unit.
Refer to note in the operating in-
structions, page ...
Symbols on the Unit:
CE symbol
Note the operating instructions!
Observe the operating instructions!
VDE-tested safety
(nameplate)
1. Information regarding Safe Operation
Symbols in the Short Instructions:
Check humidifying system water
level and top up as required. Max.
fill volume, 4.5 l; min. volume, 1.8 l
Fill 350 ml water reservoir for Con-
traCon disinfection routine
Initiate auto-start. Press and hold
button for at least 5 seconds.
Initiate ContraCon disinfection rou-
tine. Press and hold button for at
least 5 seconds.
Set desired temperature and CO2
concentration.
Clean sample chamber.
HERAcell®240 CO2Incubator Operating Instructions
- 10 - Version: 03.2001 / 50065640
Improper Use:
Cell and tissue cultures which do not meet the requi-
rements of safety levels L1, L2, and L3 may not be
used in the unit.
Tissue, materials, or liquids:
•Which are highly flammable or potentially ex-
plosive;
•Whose vapors form flammable or explosive
mixtures with air;
•Which release toxins;
may not be used.
1. Information regarding Safe Operation
1.4
General Safety Information
Proper Use:
The HERAcell®240 CO2Incubator is a laboratory unit
used for the cultivation of cell and tissue cultures.
The unit permits the simulation of the special physio-
logical environmental conditions for these cultures by
means of the exact regulation of:
•Temperature;
•CO2content, and;
•The adjustment of an elevated relative humi-
dity.
It is equipped with an automatic decontamination rou-
tine.
The HERAcell®240 CO2Incubator is intended to pro-
vide an environment with controlled temperature, CO2,
elevated humidity and automatic decontamination
mode for the development of ova or embryos at or
near body temperature.
The HERAcell®240 is suitable for installation and
operation in the following areas:
•Laboratories performing cellular biology and
biotechnology work and conforming to safety
levels L1, L2, and L3.
•Medical/microbiological laboratories confor-
ming to DIN 58 956.
•Laboratories in the central areas of clinics and
hospitals.
The CO2Incubator is suitable for continuous opera-
tion.
The CO2used to supply the incubator is provided by a
separate gas supply system, either from a CO2gas
cylinder or a central compressed gas container. The
gas supply equipment must be designed so that the
operating pressure in the CO2supply line can be ad-
justed to a range of 0.8 bar min. to 1 bar max. and
cannot be altered.
HERAcell®240 CO2Incubator Operating Instructions
Version: 03.2001 / 50065640 - 11 -
Disposal
Old units or no longer serviceable components con-
tain materials which can be recycled.
CAUTION – Contamination Hazard!
The unit may be used to process infec-
tious substances. Therefore, the unit or its
individual components may be contamina-
ted.
All unit components must be decontami-
nated prior to disposal!
•Clean all unit components thoroughly then,
depending of the application, either disinfect
or sterilize them.
•A declaration of freedom from hazards, inclu-
ding information regarding the decontaminati-
on measures performed, must accompany the
goods destined for disposal.
After proper decontamination, all components can be
passed on to regular disposal/recycling channels.
For detailed information regarding employed materi-
als, please refer to Chapter 14 “Employed Materials”.
NOTE –Recycling-Service:
Kendro offers a proper recycling service for
old equipment. For further information regarding this
fee-based service, please contact the Kendro Service.
1. Information regarding Safe Operation
Safety Regulations:
The following safety regulations apply to the operation
of the unit in the Federal Republic of Germany:
•ZH 1/10
•ZH 1/119
•ZH 1/342
•ZH 1/343
•ZH 1/598
•TRG 280
•Safety data sheets from the gas suppliers with
regard to the special properties of CO2.
•“Basics of Good Microbiological Techniques”,
information sheet issued by the Federal As-
sociation of the Chemical Industry.
The corresponding national regulations are binding in
other countries.
Safety Requirements:
The unit conforms to the following safety require-
ments:
•EN 61010
•Low-voltage Guideline, 73/23 EWG
•EMP Guideline, 89/336 EWG
•DIN 12880, Part 1/11.78
Protective Equipment:
The unit is equipped with several safety devices:
•An interlock switch in the door interrupts the
CO2supply and the heater when the glass
door is opened.
•An independent overtemperature protection
protects the samples against damaging ex-
cess temperature in case of a fault.
•A pressure compensation opening provides
pressure compensation for the sample cham-
ber.
•Audible and optical warning indicators signal
faults during operation.
HERAcell®240 CO2Incubator Operating Instructions
- 12 - Version: 03.2001 / 50065640
1. Information regarding Safe Operation
1.5
Safety Information Concerning CO
2
CO2is classified as a gas representing a potential
health hazard. Special safety measures must therefo-
re be observed when starting and using the CO2 Incu-
bator.
NOTE – Instruction:
Prior to beginning their work, all personnel in-
volved with operating units with CO2supply must be
instructed in the special requirements for handling
CO2:
•Proper operation of compressed air contai-
ners and gas supply equipment (e.g., TRG
280);
•Obligation to report damage and deficiencies
in the CO2supply lines;
•Measures to be instituted in case of accidents
and faults.
Instruction is to be repeated at suitable intervals. All
special instructions provided by the gas supplier must
be included in the instruction.
NOTE – Installation Work:
Only qualified personnel using suitable tools
may work on supply lines and compressed gas con-
tainer, bottles, or collective systems in which CO2is
stored for use with the incubator.
CAUTION – Suffocation Hazard!
The release of larger amounts of CO2into
the immediate environment will result in a
suffocation hazard.
Immediately initiate the planned safety
measures in case of a CO2leak!
•Leave the room immediately and prevent the
entry of other personnel!
•Immediately inform the local emergency servi-
ces or fire department!
HERAcell®240 CO2Incubator Operating Instructions
Version: 03.2001 / 50065640 - 13 -
2.2
Receipt Inspection
As soon as possible after receiving the unit check the
unit components for any signs of shipping damage
and make sure the delivery is complete.
Should damage be discovered or if components are
missing, immediately inform the carrier, Kendro Labo-
ratory Products, or your dealer.
2. Initial Inspection
2.1
Packaging
The HERAcell®240 CO2Incubator is delivered in a ri-
gid shipping crate. All packing materials can be sepa-
rated for recycling:
Packing material
•Box
Recycled paper
•Plastic foam Styrofoam (CFC free)
•Pallet Untreated wood
•Wrapping Polyethylene
•Straps Polypropylene
HERAcell®240 CO2Incubator Operating Instructions
- 14 - Version: 03.2001 / 50065640
2. Initial Inspection
2.3
Parts supplied
CO2Incubator with full glass door CO2Incubator with 6-port gas diaphragm
Equipment level Full glass door with
continuous inserts
(standard version)
Full glass door with di-
vided inserts 6-port gas diaphragm
with divided inserts 6-port gas diaphragm
with continuous inserts
Insert tray 3663
Insert tray support pro-
files 4664
Insert tray support
bracket 6 12 12 6
Drawer 1111
Pressure compensation
opening insert 1111
Access port plug 1111
Power cord 1111
Connector, zero-
potential contact 1111
Spare cover flaps, set 1111
CO2connecting hose
set 1111
Immersion water pump 1111
Tools
Open-end wrench,
24 mm 1111
Allen wrench, 2 mm,
for fan wheel 1111
Allen wrench, 3 mm, for
fan cover 1111
Instructions
Operating Instructions 1111
Short Instructions 2222
HERAcell®240 CO2Incubator Operating Instructions
Version: 03.2001 / 50065640 - 15 -
3. Location Requirements
3.2
Room Ventilation
When CO2is fed to the unit, there is a slight over-
pressure in the sample chamber. Excess gas exits the
incubator through the pressure compensation opening
and when the door is opened, thus equalizing the
pressure inside and outside the unit.
The pressure compensation opening and opening the
door during operation will release very small
amounts of CO2 into the surrounding atmosphere.
The room ventilation must be able to vent this gas
safely to the outside.
In addition, when used continuously, the heat gene-
rated by the unit may alter the ambient conditions.
•Therefore, always set the HERAcell®240 up in an
area with adequate ventilation.
•Do not set the unit up in corners or other inade-
quately ventilated areas.
•The room ventilation system should conform to
the requirements of ZH 1/119 (laboratory guideli-
nes).
3.1
Ambient Conditions
The unit may only be operated at setup locations
which conform to the special ambient conditions listed
below:
Requirements:
•The setup location must be dry and free of drafts.
•The minimum distances to adjacent surfaces must
be maintained on all sides. Refer to Section 3.3.
•The room must be equipped with adequate venti-
lation. Refer to Section 3.2.
•The setup surface must be firm, level, and non-
flammable.
•The support frame (base, laboratory workbench)
must have adequate bearing strength and be vi-
bration free to withstand the weight of the unit,
both empty and loaded (particularly if units are to
be stacked).
•In order to achieve a proper, constant incubation
temperature of 37 °C, the ambient temperature
must remain in the range between +18 °C to +33
°C.
•The relative humidity must not exceed 80 %.
•The unit must be set up where it will not be in di-
rect sunlight.
•No unit capable of generating large amounts of
heat should be placed near the HERAcell®240.
HERAcell®240 CO2Incubator Operating Instructions
- 16 - Version: 03.2001 / 50065640
3. Location Requirements
Figure 1
Fig. 1, Unit dimensions and minimum distances.
Information given in mm; F = Front
3.3
Space Requirements
When setting up the unit, make sure the installation
and supply lines remain freely accessible.
The circuit cabinet on the rear of the unit can be used
as a spacer with regard to adjacent objects.
The dimensions provided regarding space and clea-
rances required are to be considered the minimum re-
quirements.
NOTE – Unit Accessibility:
We recommend maintaining larger than spe-
cified distances on the sides and rear of the unit to
make access for care and maintenance tasks easier.
To avoid contamination of the CO2Incubator, a base
or support should always be used, even when the unit
is installed at floor level.
Kendro offers various stands as part of its line of ac-
cessory equipment (Refer to Chapter 12, “Spare Parts
and Accessories” for the relevant order number).
HERAcell®240 CO2Incubator Operating Instructions
Version: 03.2001 / 50065640 - 17 -
3. Location Requirements
Figure 2 / 3
Fig. 2, Lift points
Fig. 3, Stacking
3.4
Moving
NOTE – Lift Points:
When moving the unit, do not lift it by its doors
or other installed components such as the circuit cabi-
net on the rear.
Only lift the unit at the lift points indicated in the figure.
3.5 Stacking
A maximum of two HERAcell®240 units can be stak-
ked. For stacking, you will need the adapter panel
(50068677) which provides a thermal barrier between
the two units.
Stacking elements [2] are provided on the top of the
unit and the top of the adapter panel. The unit feet [1]
on the underside of the unit and the underside of the
adapter panel mate with these elements to ensure a
secure hold for the top unit.
When stacking units, a base stand with a minimum
height of 185 mm should be employed to prevent
contamination of the CO2Incubator.
If the units are mounted on a movable base or cart,
make sure that the base or cart has locking castors so
that they can be locked and pointed forward when the
incubator is put in its operating position, as this offers
the highest degree of safety.
NOTE – Moving Stacked Units:
The stacking elements are not equipped so
that they lock together. Because of this care must
be taken when moving the stacked incubators parti-
cularly. Ramps and uneven surfaces should be avoi-
ded.
HERAcell®240 CO2Incubator Operating Instructions
- 18 - Version: 03.2001 / 50065640
3. Location Requirements
Figure 4
Fig. 4, Drawer installation
3.6
Door Modifications
Both the exterior door and the glass door can be in-
stalled so that they open to the left or right. The door
stop can be retrofitted.
Standard versions of the incubator that have the full
glass door can be modified to have a 6-port divided
gas diaphragm. In this case, the gas diaphragm re-
places the glass door.
NOTE – Modifications:
Door modifications may only be performed by
the Kendro Laboratory Products Service.
3.7 Drawer
Once the unit has been set up, the drawer is inserted
between the two guide brackets under the unit body.
1. Completely open the exterior unit door to its stop.
2. Swing guide bracket [1] completely down.
3. Insert the drawer [4] with its raised edge [2] be-
hind the cable conduit [3]. The short instructions
[5] should face the front of the unit.
4. Slightly raise the front of the drawer and slide it
downwards and into place.
5. Push the drawer in to its pressure point, then
press down on the guide bracket and push it
completely back to its stop.
HERAcell®240 CO2Incubator Operating Instructions
Version: 03.2001 / 50065640 - 19 -
4.1
Front View
[1] Stacking elements
[2] Cover flaps
[3] Measuring cell with fan wheel and sensors
[4] Door interlock
[5] Glass door
[6] Measurement opening
[7] Exterior door
[8] Exterior door seal, replaceable
[9] Foot, for height adjustment
[10] Drawer, pull-out
4. Parts Identification
Fig. 5, Front view
Figure 5
[11] Nameplate
[12] Main power switch
[13] Support profile
[14] Insert tray
[15] Door lock, glass door
[16] Support bracket
[17] Pressure compensation opening with insert
[18] Access port with plug
[19] Seal, glass door, replaceable
2
2
9
8
10
11
12
13
15
16
17
18
19
14
5
4
3
1
6
7
HERAcell®240 CO2Incubator Operating Instructions
- 20 - Version: 03.2001 / 50065640
4. Parts Identification
4.2
Rear View
[1] Circuit cabinet with supply connections:
•Gas connection
•RS 232 interface
•Zero-potential alarm contact
•Mains power connection
[2] Access port, 42 mm dia.
[3] Pressure compensation opening
Figure 6
Fig. 6, Rear view
Table of contents
