Kendro HERAcell 240 User manual

Operating Instructions
HERAcell
®
240 CO
2
Incubator
with Decontamination Routine
Version: 03.2001 / 50065640

HERAcell®240 CO2Incubator Operating Instructions
- 2- Version: 03.2001 / 50065640

HERAcell®240 CO2Incubator Operating Instructions
Version: 03.2001 / 50065640 - 3-
Contents
1. Information regarding Safe Operation...............................................................................................................
6
1.1 Operator Information.....................................................................................................................................
6
1.2 User Information ...........................................................................................................................................
7
1.3 Explanation of Symbols ................................................................................................................................
8
1.4 General Safety Information.........................................................................................................................
10
1.5 Safety Information Concerning CO2............................................................................................................
12
2. Initial Inspection.................................................................................................................................................
13
2.1 Packaging ...................................................................................................................................................
13
2.2 Receipt Inspection ......................................................................................................................................
13
2.3 Parts supplied............................................................................................................................................. 1
4
3. Location Requirements.....................................................................................................................................
15
3.1 Ambient Conditions.....................................................................................................................................
15
3.2 Room Ventilation.........................................................................................................................................
15
3.3 Space Requirements ..................................................................................................................................
16
3.4 Moving.........................................................................................................................................................
17
3.5 Stacking ......................................................................................................................................................
18
3.6 Door Modifications......................................................................................................................................
18
3.7 Drawer.........................................................................................................................................................
18
4. Parts Identification.............................................................................................................................................
19
4.1 Front View...................................................................................................................................................
19
4.2 Rear View....................................................................................................................................................
20
5. System Description ...........................................................................................................................................
21
5.1 Sample Chamber Atmosphere ...................................................................................................................
21
5.2 ContraCon Decontamination Routine.............................................................................................................
21
5.3 Sensors.......................................................................................................................................................
22
5.4 Door Interlock Switch..................................................................................................................................
22
5.5 Supply Connections....................................................................................................................................
23
5.6 Sample Chamber Layout............................................................................................................................
24
6. Initial Setup.........................................................................................................................................................
26
6.1 Sample Chamber Preparation....................................................................................................................
26
6.2 Rack System Installation.............................................................................................................................
26
6.3 Gas Connection..........................................................................................................................................
29
6.4 Power Connection.......................................................................................................................................
30
6.5 RS 232 Interface Connection......................................................................................................................
31
6.6 Alarm Contact Connection..........................................................................................................................
33
6.7 Cleaning......................................................................................................................................................
35
7. Control, Displays, Indicators............................................................................................................................
36
7.1 Main Power Switch .....................................................................................................................................
36
7.2 Control Panel..............................................................................................................................................
36
7.3 Microprocessor Self-test.............................................................................................................................
37
7.4 Temperature Setpoint Specification............................................................................................................
38
7.5 CO2Setpoint Specification..........................................................................................................................
39
7.6 Setting the High/Low Humidity....................................................................................................................
39
7.7 auto-start Activation....................................................................................................................................
40
7.8 Error Codes.................................................................................................................................................
41
7.9 Error Code Overview ..................................................................................................................................
43
7.10 Resetting the Overtemperature Protector...................................................................................................
44
8. Operation...........................................................................................................................................................
45
8.1 Preparing the Unit.......................................................................................................................................
45
8.2 Beginning Operation...................................................................................................................................
45
9. Decommissioning..............................................................................................................................................
46
9.1 Taking the Unit Out of Service....................................................................................................................
46

HERAcell®240 CO2Incubator Operating Instructions
- 4- Version: 03.2001 / 50065640
Contents
10. Cleaning and Disinfection.................................................................................................................................
47
10.1 Preparation .................................................................................................................................................
47
10.2 Cleaning......................................................................................................................................................
49
10.3 Manual Disinfection.....................................................................................................................................
49
10.4 ContraCon Decontamination Routine.............................................................................................................
51
10.5 ContraCon Decontamination Routine Activation ........................................................................................
53
10.6 Interrupting the ContraCon Decontamination Routine................................................................................
53
11. Maintenance.......................................................................................................................................................
54
11.1 Inspections and Checks..............................................................................................................................
54
11.2 Maintenance Intervals.................................................................................................................................
54
11.3 Checking the Temperature.........................................................................................................................
55
11.4 Equalizing the Temperature........................................................................................................................
56
11.5 Checking the CO2Concentration................................................................................................................
57
11.6 Equalizing the CO2Concentration..............................................................................................................
58
11.7 Sterile Filter Replacement for the CO2Gas Supply....................................................................................
59
11.8 Unit Fuse Replacement ..............................................................................................................................
60
11.9 Door Seal Replacement..............................................................................................................................
60
12. Spare Parts and Accessories...........................................................................................................................
61
12.1 List of Spare Parts and Accessories ...........................................................................................................
61
13. Technical Specifications...................................................................................................................................
63
14. Materials Employed...........................................................................................................................................
65
Attachments
A Principles of Good Microbiology Techniques................................................................................................
66
B Unit Log ..............................................................................................................................................................
68
Addresses...................................................................................................................................................................
70

HERAcell®240 CO2Incubator Operating Instructions
Version: 03.2001 / 50065640 - 5-
Index of Figures
Fig. 1 Unit dimensions and minimum distances..................................................................................................
16
Fig. 2 Lift points ...................................................................................................................................................
17
Fig. 3 Stacking.....................................................................................................................................................
17
Fig. 4 Drawer installation.....................................................................................................................................
18
Fig. 5 Front view..................................................................................................................................................
19
Fig. 6 Rear view...................................................................................................................................................
20
Fig. 7 Metering cell sensors.................................................................................................................................
22
Fig. 8 Door interlock switch .................................................................................................................................
22
Fig. 9 Supply connections ...................................................................................................................................
23
Fig. 10 Sample chamber layout...........................................................................................................................
24
Fig. 11 Water reservoir........................................................................................................................................
25
Fig. 12 Rear unit wall openings...........................................................................................................................
25
Fig. 13 Support profile installation/removal .........................................................................................................
26
Fig. 14 Support bracket / insert tray installation..................................................................................................
27
Fig. 15 Center support installation.......................................................................................................................
28
Fig. 16 Gas supply line connection......................................................................................................................
29
Fig. 17 Power connection....................................................................................................................................
30
Fig. 18 RS 232 interface......................................................................................................................................
31
Fig. 19 Example of a connection diagram for an external alarm system ............................................................
33
Fig. 20 Switch the unit on/off...............................................................................................................................
36
Fig. 21 Control panel functions............................................................................................................................
36
Fig. 22 Protective screen/fan wheel disassembly ...............................................................................................
48
Fig. 23 Schematic diagram of the decontamination routine process ..................................................................
52
Fig. 24 Temperature equalization........................................................................................................................
55
Fig. 25 CO2equalization......................................................................................................................................
57
Fig. 26 Sterile filter replacement..........................................................................................................................
59
Fig. 27 Unit fuse replacement..............................................................................................................................
60
Fig. 28 Door seal replacement ............................................................................................................................
60

HERAcell®240 CO2Incubator Operating Instructions
- 6- Version: 03.2001 / 50065640
1. Information regarding Safe Operation
1.1
Operator Information
The HERAcell®240 CO2Incubator has been manu-
factured according to the highest standards and is
operationally safe. Nonetheless, this unit presents a
source of potential hazard, particularly when used by
individuals with inadequate instruction or if it is used in
an improper manner or for work for which it is not in-
tended.
•Using these operating instructions, the safety data
sheets, hygienic guidelines, and in compliance
with applicable technical regulations, the operator
must prepare written instructions in an under-
standable form for the users of this unit.
•These instructions are to be provided in the lan-
guage of the employees.
•Using these instructions, instruct the operating
and cleaning personnel in the functions, the ope-
ration, and the required cleaning tasks for this
unit.
•The content of these operating instructions is
subject to change at any time without notification.
•The German version of these operating instructi-
ons remains binding for all translations.
•Carefully store these operating instructions near
the unit so that operating personnel can refer to
them with regard to the safety information and im-
portant operating information.
For your own safety, please contact Kendro Laborato-
ry Products or your dealer should any questions arise
which are not adequately covered by these operating
instructions.
Germany
Address:
Kendro Laboratory Products GmbH
Heraeusstr. 12-14
D - 63450 Hanau, Germany
Deutschland
Telephone:
Sales + 49 (0) 1805-536376
Service + 49 (0) 1805-112110
Fax:
Sales / Service + 49 (0) 1805-112114

HERAcell®240 CO2Incubator Operating Instructions
Version: 03.2001 / 50065640 - 7-
1. Information regarding Safe Operation
Warranty:
Kendro Laboratory Products only warrants the safety
and operability of the HERAcell®240 CO2Incubator
under the condition that:
•The unit is employed solely for its intended purpo-
se and is operated and maintained in accordance
with the information in these operating instructi-
ons;
•No structural alterations are made to the unit;
•Only original spare parts and accessories appro-
ved by Kendro Laboratory Products are used;
•All inspection and maintenance tasks are perfor-
med at the specified intervals.
The warranty period begins at the time the unit is deli-
vered to the ordering party.
Trademarks:
Heraeus, HERAcell®are registered trademarks of
Kendro Laboratory Products GmbH. Any other trade-
marks cited in these operating instructions are the
sole property of the associated manufacturers.
Copyright©
These operating instructions are copyrighted. Reprin-
ting, photomechanical or digital processing or repro-
duction – including extracts – are therefore only
permitted with the written approval of Kendro Labora-
tory Products GmbH.
This does not apply to the reproduction for use solely
within the operator’s company.
1.2
User Information
It is important for you to read these operating instruc-
tions carefully before using the unit for the first time.
This will allow you to exploit all the advantages the
unit offers and to protect individuals, samples, and the
environment against possible harm:
•These operating instructions describe the HERA-
cell®240 CO2Incubator.
•The CO2Incubator may only be operated by
trained, authorized personnel.
•Maintenance work on the unit may only be per-
formed by the Kendro Laboratory Products Ser-
vice or trained, authorized agents.

HERAcell®240 CO2Incubator Operating Instructions
- 8- Version: 03.2001 / 50065640
1.3
Explanation of Symbols
Symbols Used in These Operating Instructions:
WARNING!
Failure to observe this instruction can result in severe
or fatal injury.
CAUTION!
Failure to observe this instruction can result in slight to
serious injury or property damage.
NOTE!
Indicates important information and application tips.
RECYCLING!
Valuable raw materials can be recovered.
Wear protective gloves!
Wear safety glasses!
Hazardous liquids!
Electrical shock hazard!
Hot surface!
Fire hazard!
1. Information regarding Safe Operation
Symbols in the Short Instructions:
Operator steps to be carried out on
the unit
Unit display status
Switch the unit on.
Switch the unit off.
Open the unit doors.
Leave the unit doors open for at
least 30 seconds.

HERAcell®240 CO2Incubator Operating Instructions
Version: 03.2001 / 50065640 - 9-
Symbols in the Short Instructions:
Close unit doors.
Remove samples and water reser-
voir from the unit.
Load unit.
Refer to note in the operating in-
structions, page ...
Symbols on the Unit:
CE symbol
Note the operating instructions!
Observe the operating instructions!
VDE-tested safety
(nameplate)
1. Information regarding Safe Operation
Symbols in the Short Instructions:
Check humidifying system water
level and top up as required. Max.
fill volume, 4.5 l; min. volume, 1.8 l
Fill 350 ml water reservoir for Con-
traCon disinfection routine
Initiate auto-start. Press and hold
button for at least 5 seconds.
Initiate ContraCon disinfection rou-
tine. Press and hold button for at
least 5 seconds.
Set desired temperature and CO2
concentration.
Clean sample chamber.

HERAcell®240 CO2Incubator Operating Instructions
- 10 - Version: 03.2001 / 50065640
Improper Use:
Cell and tissue cultures which do not meet the requi-
rements of safety levels L1, L2, and L3 may not be
used in the unit.
Tissue, materials, or liquids:
•Which are highly flammable or potentially ex-
plosive;
•Whose vapors form flammable or explosive
mixtures with air;
•Which release toxins;
may not be used.
1. Information regarding Safe Operation
1.4
General Safety Information
Proper Use:
The HERAcell®240 CO2Incubator is a laboratory unit
used for the cultivation of cell and tissue cultures.
The unit permits the simulation of the special physio-
logical environmental conditions for these cultures by
means of the exact regulation of:
•Temperature;
•CO2content, and;
•The adjustment of an elevated relative humi-
dity.
It is equipped with an automatic decontamination rou-
tine.
The HERAcell®240 CO2Incubator is intended to pro-
vide an environment with controlled temperature, CO2,
elevated humidity and automatic decontamination
mode for the development of ova or embryos at or
near body temperature.
The HERAcell®240 is suitable for installation and
operation in the following areas:
•Laboratories performing cellular biology and
biotechnology work and conforming to safety
levels L1, L2, and L3.
•Medical/microbiological laboratories confor-
ming to DIN 58 956.
•Laboratories in the central areas of clinics and
hospitals.
The CO2Incubator is suitable for continuous opera-
tion.
The CO2used to supply the incubator is provided by a
separate gas supply system, either from a CO2gas
cylinder or a central compressed gas container. The
gas supply equipment must be designed so that the
operating pressure in the CO2supply line can be ad-
justed to a range of 0.8 bar min. to 1 bar max. and
cannot be altered.