Key Diagnostics DOSIPUMP DP 1000 Series User manual

DosiPump 1000 User Manual
Page 1 / 24 Version 1.01E
D OSI P UMP DP 1000
DosiPump DP1000 Item # 730’000
DosiPump DP1000 Fast Item # 740’000
U SER M ANUAL 1.01-E
Version 2010 / 2011

DosiPump 1000 User Manual
Page 2 / 24 Version 1.01E
Contents
1 OVERVIEW......................................................................................................................................... 3
1.1 PREFACE FURTHER TO THE USER MANUAL ................................................................................. 3
1.2 DOSIPUMP USER MANUAL VERSION 1.01.................................................................................... 3
1.3 BASIC INFORMATION ON THE DOSIPUMP 1000............................................................................ 4
2 SAFETY INFORMATION ................................................................................................................. 6
2.1 OPERATING ENVIRONMENT ........................................................................................................... 6
2.2 SAFETY-CONSCIOUS WORKING..................................................................................................... 6
2.3 QUALIFICATION OF THE OPERATING PERSONNEL ......................................................................... 6
2.4 SAFETY INFORMATION FOR THE USER COMPANY ......................................................................... 7
2.5 NO ARBITRARY CONVERSION WORK OR MODIFICATION WORK ..................................................... 7
2.6 SUPPLEMENTARY SAFETY INFORMATION...................................................................................... 7
2.7 WARRANTY AND LIABILITY............................................................................................................. 7
3 COMMISSIONING ............................................................................................................................. 8
3.1 UNPACKING ................................................................................................................................... 8
3.2 MAINS CONNECTION...................................................................................................................... 8
3.3 INSERTING THE TUBE FITTING IN THE PUMP .................................................................................. 8
3.4 METAL BAR FIXATION MAKING THE STURDY HOUSING A STABLE STAND ...................................... 8
3.5 MAIN SWITCH................................................................................................................................ 8
4 OPERATION OF THE DOSIPUMP 1000 ...................................................................................... 9
4.1 FUNDAMENTALS ............................................................................................................................ 9
4.2 DIRECT DOSING .......................................................................................................................... 10
4.3 EXECUTE DOSING PROGRAM ..................................................................................................... 11
4.4 EXECUTION SCREEN................................................................................................................... 12
4.5 EDIT /CREATE PROGRAM........................................................................................................... 13
4.6 CALIBRATION OF TUBES.............................................................................................................. 14
4.7 SYSTEM MENU ............................................................................................................................ 15
5 TECHNICAL INFORMATION ........................................................................................................ 16
5.1 INTRODUCTION ............................................................................................................................ 16
5.2 CLEANING.................................................................................................................................... 16
5.3 TRANSPORTING THE DOSIPUMP 1000....................................................................................... 16
5.4 CALIBRATION............................................................................................................................... 17
5.5 TROUBLESHOOTING .................................................................................................................... 17
5.6 ELECTRICAL CONNECTIONS ........................................................................................................ 19
5.7 TECHNICAL DATA......................................................................................................................... 20
6 ADDITIONAL APPLICATION DATA ........................................................................................... 21
6.1 ACCESSORIES &OPTIONS.......................................................................................................... 21
6.2 REPRODUCIBILITY AND PRECISION............................................................................................. 22
6.3 CERTIFICATES ............................................................................................................................. 23
6.4 PRODUCT REGISTRATION ........................................................................................................... 23
6.5 CONTACTS &FEEDBACK ............................................................................................................ 23

DosiPump 1000 User Manual
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1 Overview
1.1 Preface further to the User Manual
The User Manual explains the mode of operation and the operating procedures for the D OSI P UMP 1000.
The User Manual is subdivided into five main sections:
1. Overview
2. Safety information
3. Commissioning
4. Operation of the DosiPump 1000 / Software Version 2.0 or higher
5. Technical information
6. Additional User Information
Each section consists of a guide to the individual functions, structured step-by-step. The user is also
provided with special information relating directly to the relevant task explained (Warning, Important and
Note).
Overview
The Overview contains general information on the D
OSI P UMP 1000, the intended application and the
basic concept.
Safety Information
The D
OSI P UMP DP1000 safety information.
Commissioning
The steps and preparatory work required for commissioning.
Operation of the D
OSI P UMP 1000
This section documents all information required for daily operation. It explains both operation and all pa-
rameters.
Technical information
This section provides comprehensive technical information on all functions of the D
OSI P UMP 1000. This
section is structured so that it can be used both as a source of additional useful information on operation
and as an independent reference work.
Additional User Information
This section provides comprehensive application notes
1.2 Dosipump User Manual Version 1.01
Review August 1
st
/ BioTool AG_JNM
Review November 1
st
/ BioTool AG_JNM

DosiPump 1000 User Manual
Page 4 / 24 Version 1.01E
Peristaltic Pumps Principle
Peristaltic pumps work by reduction of a volume
within a tube by a series of rollers. The fluid is forced
through tubing by a number of rollers, which rotate
squeezing a flexible tube against the
pump housing
+ Large Flows range
+ Self priming
+ Tube selection (autoclavable)
+ Easy cleaning
+ No carry over (different tubes)
+ Reversible (DC)
+ Low maintenance
- Volume variation from tube to tube
- Regular tube calibration required
1.3 Basic information on the D OSI P UMP 1000
1.3.1 Introduction to the D OSI P UMP 1000
Figure 1: D
OSI P UMP 1000
I
TEM N O . 730000 AND 740000 (F AST )
1.3.2 Basic concept
The D
OSI P UMP 1000 is an easy to handle multi-functional Peristaltic Laboratory Pump offing a wide
range of functions and a wide dosing range in only 1 unit. Precision and reproducibility are depending
the ratio between tube and desired volume. The smaller the tube the higher the precision and reproduci-
bility, but the smaller the pump speed. Due to its sturdy metal double pump head DosiPump accepts a
high range of tubing’s, starting with 2 x 6mm (inner/outer tube diameter) for small, precise dosings, up to
double tube sets 8 x 12 mm for high speed dosings. Thanks to its double pump head with 2 x 5 special
rolls, DosiPump eliminates most pulsation by balancing the typical peristaltic pulsation-peaks by deferred
pump peaks.
1.3.3 Intended application
The D
OSI P UMP 1000 offers the user a wide variety of functions and features.
Features:
•Easy-to-understand, user-friendly operation with graphic screen and touch screen
•Programming by simply editing the step by step protocol listed in the internal spread sheet
DosiPump 10 Roller Double-channel Pump Head
Cannel 1Channel 2 Mean Flow Regular Dosing Pulse of 3 Roller Pump Head

DosiPump 1000 User Manual
Page 5 / 24 Version 1.01E
•Variably adjustable sample volumes, speed, pause time and AntiDrop function
•Automatic initialisation and self-test run
•Up to 8 different tube calibrations can be stored and are available in any program
•The tube fittings can be exchanged easily thanks to the practical lever closure on the pump head
•Self monitoring of all device functions (motors ...)
•Easy maintenance, full stainless steel housing
•Host PC connection for protocol printing (Optional Recorder Software #700290, Cable#700280)
•External printer connection (Optional DosiPrint #750000+Cable #750115)
•External control with footswitch (101010)
Benefits:
DosiPump allows straight forward dosing directly from the unit main screen.
DosiPump allows easy call of any program combined with any previously stored tube value.
DosiPump allows to easily editing of even complex dosing-rows in a well arranged spread sheet.
Any individual dosing value can be defined for any individual dose, such as pump direction, pump
speed, pause, anti drop angle repeats e.g.
DosiPump accepts tube sizes from 2x6mm for small doses up to a double tube set of 8x12mm for
fast dosing
DosiPump is build in a stable stainless steel housing offering IP64 splash water resistance.
DosiPump DP1000 models:
•Pump Range: 5ml (1ml +0.1ml steps) - 800ml/min depending tube size Item No. 730’000
•Pump Range: 15ml (1ml +0.1ml steps) -2’400ml/min depending tube size Item No. 740’000
1.3.4 Symbols used in the User Manual
The signal words WARNING, IMPORTANT and NOTE, used in this User Manual, serve to highlight im-
portant information and instructions applicable to danger situations. These words can be found together
with a special warning symbol in the left-hand column.
Symbol Definition
Warning points out a potentially dangerous situation which should be avoided in order
to avoid damage to the equipment or injuries. This signal word is used only in extreme
situations and thus requires special attention.
Important points out special problems or important information. Please read through
the accompanying text carefully. It is important for understanding the relevant topic or
command.
Note serves to identify information which is useful but not crucial to a task. Read
though the accompanying text carefully. It may help you to clarify any questions which
you may have.

DosiPump 1000 User Manual
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2 Safety information
2.1 Operating environment
Several factors must be noted when selecting the right operating environment for the unit. The room se-
lected may not pose an aggressive environment either for the unit itself or for its intended application.
The D
OSI P UMP 1000 should be operated only under ambient conditions corresponding to a normal envi-
ronment (5°C with maximum 80 % relative humidity).
NOTE These conditions also apply to transport.
The D
OSI P UMP 1000 should be installed on a clean, dry, smooth, horizontal surface. The front panel of
the unit should be near to the front edge of the workbench in order to ensure convenient operation and
easy access to the touch screen. The unit should be installed in the direct vicinity of electrical socket
outlets.
Summary of the D
OSI P UMP 1000 operating environment
Temperature range: +5°C to +45°C
Relative humidity: Max. 80 % up to 31°C; decreasing in linear fashion down to 50 % at 40°C
Mains power supply: Voltage fluctuations +/- 10 % of rated value
Pollution severity: 2 in accordance with IEC 664
Location: Complying with enclosure IP 21
Sturdy support surface
Environment: Avoid splash water and contact with chemicals.
DosiPump is not an EX Approved
WARNING We are unable to guarantee operability and functional safety of the unit if
the ambient conditions applicable to the location are not complied with.
2.2 Safety-conscious working
The D
OSI P UMP 1000 complies with the state of the art and is safe to operate. The D OSI P UMP 1000 may
pose residual risks and dangers if it is used and operated incorrectly by untrained personnel. All persons
entrusted with the task of operating the D
OSI P UMP 1000 must have read and understood the User Manu-
al and, in particular, the safety information or must have been instructed by superiors in such a manner
that they are able to safely operate the D
OSI P UMP 1000.
Besides the safety information listed above, other applicable provisions and regulations such as GLP,
GMP, FDA, the regulations of the German Liability Insurance Associations, those of the Heath Authority
and those of the Factory Inspectorate must also be followed.
2.3 Qualification of the operating personnel
Specialist technical training is generally required. Special knowledge is required when operating the
D
OSI P UMP 1000 to prevent potentially dangerous situations with the unit. Non skilled staff without the
corresponding specialist technical training generally do not have such qualifications and are able to per-
form these tasks only after instruction and under the supervision of specialist personnel.

DosiPump 1000 User Manual
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2.4 Safety information for the user company
The superiors of the operating personnel must have understood and must comply with Chapter "Safety
information" and the aspects of operation relevant to safety. They must ensure that the operating per-
sonnel have understood and follow the safety information.
Before placing the unit into operation, superiors must check that operation of the D
OSI P UMP 1000 in con-
junction with other installations or system sections cannot pose additional risks and dangers.
If necessary, additional technical safety instructions must be drawn up by superiors. In order to preclude
the possibility of residual risks and dangers, the company using the D
OSI P UMP 1000 may need to draw
up in-company instructions and have receipt of these instructions confirmed by the personnel responsi-
ble. Moreover, the user company must stipulate the scopes of authority for working with the D
OSI P UMP
1000 so that there are clear scopes of authority in relation to technical safety aspects.
2.5 No arbitrary conversion work or modification work
No modifications may be made to the D OSI P UMP 1000. In particular, defective components may be re-
placed only by original parts. Neither the design nor the technical safety systems of the D
OSI P UMP 1000
may be modified without our express consent. In particular, no modifications to safety facilities are per-
mitted. Modifications of any nature will exempt us from liability for resultant damage and injury.
2.6 Supplementary safety information
1. The D OSI P UMP may be used only for the intended application as stated and for no other purposes.
2. Maintenance work and repair work may be carried out only by qualified staff.
3. The unit must be installed in such a manner that the On/Off switch is easily accessible at all times.
4. Should a situation arise in which safe operation of the D
OSI P UMP 1000 can no longer be guaranteed,
the unit must be placed out of operation and locked until it can be repaired by qualified staff. Such a
situation must be assumed if:
- there is visible damage to the D
OSI P UMP 1000,
- the D
OSI P UMP 1000 is no longer working or
- the mains-circuit over current release trips.
5. Disconnect the D
OSI P UMP 1000 from the mains power supply before moving it to a new location.
6. Connect the power cables of the D
OSI P UMP 1000 only to a fully earthed mains system in order to
avoid electric shock. Do not use adapters for plugs and do not remove earthing connections from
cables. If an extension cable has to be used, please use only three-core cable with correctly earthed
plugs.
7. Ensure that all cables and tubes are laid in a safe area to prevent the risk of tripping over them.
8. Do not spill food or liquids on the D
OSI P UMP 1000. Please read the technical guide if the D OSI P UMP
1000 requires cleaning.
2.7 Warranty and liability
BioTool Swiss AG provides warranty for this unit covering defects in relation to both components and
design. The unit has been thoroughly inspected in order to ensure that it complies with the specifica-
tions published. BioTool Swiss AG guarantees that this unit will remain free of defects for a period of 18
months as of the date of delivery. This warranty shall be rendered null and void if the unit is not operat-
ed by a trained person in accordance with the instructions supplied by BioTool Swiss AG or if an un-
qualified person attempts to repair the unit.
NOTE Parts subject to wear are not covered by warranty.

DosiPump 1000 User Manual
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3 Commissioning
3.1 Unpacking
The D OSI P UMP 1000 is supplied packaged in a product-specific carton. The recommended unpacking
procedure for the D
OSI P UMP 1000 is as follows:
1. Remove the securing straps
2. Open the packaging
3. Remove the inside cover of the packaging
4. Lift the unit out of the packaging. Do not grip the unit by the pump head when lifting it out.
5. Place the unit onto a sturdy table or bench.
6. Remove all miscellaneous packaging items (foam rubber and safety stickers...) and place them
back into the transport packaging since they may be needed if it becomes necessary to transport
the unit again.
WARNING If there are signs of condensation on the D
OSI P UMP 1000, allow at least two
hours for the condensation to dry out before switching on or operating the unit.
3.2 Mains connection
The D OSI P UMP 1000 must be connected to the mains power supply using the enclosed mains cable. The
electrical connection ratings must correspond to the ratings specified on the sticker on the rear side of
the unit and may be neither higher nor lower than these values.
Connect the power cable of the D
OSI P UMP 1000 only to a fully earthed mains power supply system in
order to avoid electrical shock. Do not use adapters for the plugs and do not remove the earthing con-
nections from the cable. If it is necessary to use an extension cable, please use only three-core cables
with correctly earthed plugs for this.
3.3 Inserting the tube fitting in the pump
Perform the following steps to insert the tube in the pump:
1. Move the lever on the pump head to the right
2. Insert the tube fitting into the pump
3. Move the lever back to the left
4. Immerse the end of the tube (intake end) into the medium to be dis-
pensed or connect it to the optional culture medium preparation unit.
3.4 Metal Bar fixation making the sturdy housing a stable stand
Screw on the metal bar to use the DosiPump housing as a stable stand.
1. Screw the stainless steel stand into the opening on the housing cover.
2. Attach the stainless steel cross-bar with stand cross-clamps.
3. Also firmly clamp the quick fixer clamps.
3.5 Main Switch
Press the master switch on the rear panel of the D OSI P UMP 1000. The unit is then reinitialised. If a printer
is connected, this printer is also initialised. The software version and type are displayed. You will see the
main menu after initialisation is complete.

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4 Operation of the D OSI P UMP 1000
4.1 Fundamentals
Direct Dosing; DosiPump offers a direct dispense function straight from the main screen. (4.2)
Speed & Time controlled / no absolute volumes (undefined- / not calibrated
tube set). This function allows fast, user controlled dispensing of any volume.
Programs; DosiPump allows any kind of individual pump settings for any single dose, followed
by a selectable pause and any other dose varying in speed pump direction e.g.
DosiPump simply repeats step by step any programmed values. The Programmes
are simply to enter in a spread sheet. DosiPump follows each single volume and
executes the amount of desired repeats.
Each single volume consists of the Volume (ml), the Pump speed (ml/min), the
pump direction, Antidrop (°angle / defined pump head pullback), pause before next
dose. (4.4)
All Programmes require a calibrated tube! DosiPump allows 8 Tube sets to be cal-
ibrated and selected before the execution of any Program. The Program gets
adapted automatically to the individual Tube values.
Any of the below settings can be programmed
Repeated sets of doses;
Define dose and pause (time of footswitch signal), repeat dose up
to 999 times
E.G. Dispense [9ml] [Pause] [9ml] [Pause] [9ml] [Pause] …
Repeated sets of different doses:
Up to 12 different volumes in a row,
Repeated sets of different dosings (up to 999)
E.G. Dispense [2,4,6,8,10 ml] [Pause] [2,4,6,8,10 ml] [Pause] …
Dilutions:
Aspirate defined volume, aspirate defined air-gap, dispense with
defined amount of diluent.
E.G. Asp. [1ml], + Air gap [0.5ml] Disp. [10.5ml] = 1:10 Dilution
“Pipette” Function:
Aspirate defined volume, dispense the same volume
E.G. Asp. [10ml], Disp. [10ml]
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DosiPump 1000 User Manual
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4.2 Direct Dosing
MAIN SCREEN
Step
Screenshot
1
Start Screen
Press anywhere to continue
2
3
4
DosiPump allows direct dosing straight from the main screen
(continuous pump mode). No need for previous program setting.
The speed and the pump direction can be selected.
Select Pump direction
Start / Stop
Select / adjust current pump speed
Ideal to pre-fill tubes
START / PUMP
SETTINGS
SPEED
PUMP DIRECTION
PROGRAMS

DosiPump 1000 User Manual
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4.3 Execute Dosing Program
MAIN PROGRAM SCREEN
Step Screenshot
1
2
Enter the program section by pressing this icon
Start / Stop any selected program
3
DosiPump displays the program setting
Volume (ml)
Pump Direction (front / backside)
Anti Drop (Angle in °)
Delay between singe doses (sec.)
Start Program
4
5
Select the number of repetitive dosings or endless
repeats (manual stop required)
Tube Filling option before program start
5
6
The pump displays a counter od filled doses and
simultaniously fills the status-bar
Pause / Restart Function
Stop
START PO
-
PROGRAM
SCROLL FOR
MORE
PROGRAMS
START PROGRAM
PROGRAM
PREVIOUS NEXT PAGE
EDIT PROGRAM
B
A
SKET / DELETE
ENTER / EXITMODE

DosiPump 1000 User Manual
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4.4 Execution Screen
Execution Screen
Step Screenshot
Before DosiPump starts the selected program the cycle
repetition screen appears automatically.
Select number or cycles
(repetition of complete program)
Alternatively select Endless repetitions
(repeats until user selected stop)
TARGET VOLUME
REVOLUTIONS
CURRENT DOSE
STOP PROGRAM
CURRENT SPEED
PAUSE
CURRENT VOLUME

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4.5 Edit / Create Program
Step Screenshot
DosiPump allows to type and store up to 20 different
Programs.
Selects the Program to type/edit
next Page, more Programs
Enter Program Name
(Alphanumeric pad opens)
S elects the tube (1-8)
Each single Dosing Step consists of all individual variables
a dosing can consist of.
Opens the Edit Mode of the selected step
Start / Stop dispensing this program
Volume
Pump Peed
Pump Direction
Anti-Drop Setting
Pause before jump to next dose
Enter next Volume (if different) or
Confirm entry
SCROLL FOR
MORE
PROGRAMS
TYPE / EDIT
PROGRAM
START PROGRAM
PROGRAM
PREVIOUS NEXT P
A
GE
EDIT PROGRAM
B
A
SKET / DELETE
ENTER / EXITMODE

DosiPump 1000 User Manual
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4.6 Calibration of Tubes
CALIBRATION SCREEN
Step Screenshot
Enters into the setting menu
Opens the tube calibration menu
Select a tube to be calibrated
P refil the tube and eliminate any air
bubbles in the tube.
Adjusts the desired calibration volume
Adjusts the calibration speed
EXECUTE the calibration
Follow the advices on the screen
Measure the dispensed Volume
Correct the Volume (edit current Vol.)
CHECK the calibration
Note: For best calibration results the calibration should be
executed as close to the planned dispense as possible. This
based on the physical difference any tube offers regarding
pump expansion / relaxation between the pump rolls.
Note: The pump direction in the calibration mode is CCW !
Info: The pump calculates a ratio of dispensed volume and
motor steps. It will not recognize any changes coming from
tube aging, loss of tube flexibility e.g. ! Therefore; In case of
volume drifts the tube must be recalibrated.
After the completed Calibration the calibrated tube is availa-
ble in any desired program. The selection screen indicates
the specific range for any individual tube.
Programmed Pump rates above this volume will be corrected
to the Max Value (fastest motor movement)
Pump rates below this volume are corrected to the Min Value
START / PUMP
SETTINGS
SPEED
PUMP DIRECTION
PROGRAMS

DosiPump 1000 User Manual
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4.7 System Menu
System Screen
Step Screenshot
Tube Calibration
See P4.6
4.7.1 Language
Select between English / German Menu
4.7.2 Date / Time Setting
Set Date / Time
Select European / US Display
4.7.3 Touch Screen Calibration
If the Touch Screen does not react fast and
appropriate anymore, it may be necessary
to recalibrate the touch screen.
Touch + at the top left-hand corner. Follow
the + at the bottom right-hand corner.
4.7.4 Display Contrast
Sets the display contrast.
4.7.5 About
The About menu displays the device-
specific information (required for any tech-
nical support)
4.7.6 Interface Functions (see Technical Manual)
INTERFACE
CONTRAST
ABOUT THIS UNIT
LANGUAGE
TUBE CALIBR
A
TION
DATE / TIME
INTERFACE
TOUCH

DosiPump 1000 User Manual
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5 Technical information
5.1 Introduction
Chapter "Technical information" contains additional information on operation of the D OSI P UMP 1000. It is
assumed that the unit is operated by a trained D
OSI P UMP 1000 user with the required access rights.
5.2 Cleaning
Cleaning procedures must be performed regularly in order to ensure optimum performance of the
D
OSI P UMP 1000. The cleaning routine described below must not be considered as absolutely essential. It
serves as a guideline to assist efficient operation of the D
OSI P UMP 1000. The decision on when and how
to clean must be made by the individual laboratory.
5.2.1 Materials required
•Washing-up liquid - use a mixture of one part washing-up liquid and three parts water.
•Deionised water
•Soft-lint-free cleaning cloth and paper tissues
WARNING Do not use cleaning agents containing acetone or other aggressive cleaning
agents (nitrocellulose thinners,...).
WARNING Disconnect the mains plug each time before cleaning.
5.2.2 Cleaning procedures
Cleaning the unit
•Clean the surface of the unit with a lightly moistened cloth.
•Wipe off the Plexiglas covers with a lightly moistened cloth.
Cleaning the touch screen
•The touch screen may be cleaned only with water (or soapy water). Never remove residue by
scratching or scraping the touch screen.
IMPORTANT Never use too much water since this could otherwise result in water enter-
ing the unit. Never remove residue by scratching or scraping.
5.3 Transporting the D OSI P UMP 1000
IMPORTANT Remove all tubes and disconnect the unit from the mains power supply be-
fore moving the unit.
IMPORTANT When moving the unit, always grip it at the bottom and not on the pump
head.

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5.4 Calibration
Corporate guidelines prescribe that all companies operating with a quality assurance system must regu-
larly calibrate their instruments and equipment. Calibration must ensure that the unit complies with the
stipulated specifications.
When should calibration be carried out?
Calibration relates to the dosing volume of the pump as a function of the tube.
Calibrate;
•If a new tube type is used and added to the tube calibration store for later use.
•If using culture media with greatly differing viscosities are used.
•At regular intervals, e.g. every 3 months.
•If the silicone tubing flexibility changes and results in a general volume drift. This is based on many
autoclaving cycles, standard tube flexibility loss, age, UV exposure e.g.)
NOTE The calibration procedure is described in Chapter "Calibration menu".
5.5 Troubleshooting
5.5.1 Overview
The D
OSI P UMP 1000 software incorporates troubleshooting routines which can be used to correct certain
problems without action on the part of after-sales servicing technicians.
If a problem occurs on the D
OSI P UMP 1000, the unit attempts to correct the problem by providing the op-
erator with prompts on the graphical user interface. If these on-screen prompts are followed, the fault or
error can then normally be remedied without action on the part of after-sales servicing technicians.
Each error message with alarm function also contains a description of the fault or error.
5.5.2 Fault clearance
The table below can be used to help you to remedy certain problems which may occur during operation
of the D
OSI P UMP 1000. This table contains information on the symptom, its possible causes and the re-
medial action for the relevant fault or error involved.
Errors which occur before operation
Symptom Possible cause Corrective action
Device does not start Plug not plugged in
Master switch not switched on
Master switch fuse defective
No mains power
Check power connection
Switch on
Check fuse.
See details of description.
Check power connection
Replacing the device fuse

DosiPump 1000 User Manual
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In order to replace the device fuse, use a small screwdriver to
lever the fuse holder of the power receptacle down and out.
Fuse: 3.15 A - 250 V AC - slow-blow
No fuses with other ratings may be used!
WARNING Always disconnect the mains cable first before replacing the fuse!
Faults and errors occurring during operation
Symptom Possible cause Correction
Fault signal
Pump blocked
The culture medium in the tube is too
viscous. You have used a tube which
is too large.
Remove the tube and run the pump
manually with the "PUMP" menu.
Fault signal:
No program entered
The selected program has no name
(has not been edited).
Edit the selected program and assign
it a program name.
Fault signal:
DosiPump barred
No valid license
License has expired Apply for a new license from the sell-
er with the license application code
and enter the license code.

DosiPump 1000 User Manual
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5.6 Electrical connections
The connections for the mains power supply and the optional interfaces
are located on the right-hand side of the D
OSI P UMP 1000 rear panel.
Caption
1. Footswitch Socket DIN41524 5-pin
2. PC / printer connection SUB-D socket 9-pin
3. RS485 Biolink SUB-D socket 9-pin
4. Power receptacle with fuse and master switch
1 Footswitch pin assignment
Pin 1: Footswitch contact 1
Pin 3: Footswitch contact 2
Pin 2: 29 V
The two footswitch contacts are designed as normally open contacts (NO).
2 PC / printer connection pin assignment (RS232) Version P/PI
Pin 1: RxD
Pin 2: TxD
Pin 3: GND
Pin 4: VMOT (24 V – 29 V for printer only)
3 RS485 connector pin assignment Option
Pin 1: Internal GND
Pin 3: A
Pin 7: B
Pin 9: Signal GND
NOTE Do not connect with pin assignments other than those shown above!
NOTE Use only the RS232 Host interface cable (original cable) as the interface
cable to the PC host.
NOTE Connections are provided on the device rear panel depending on options.
Only the power receptacle is provided by default.
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DosiPump 1000 User Manual
Page 20 / 24 Version 1.01E
5.7 Technical data
Length 305 mm
Depth 275 mm
Height 205 mm
Net weight 7.1 kg
Mains connections 85-132 V AC / 176-264 V AC, 47-63 Hz
Mains fuse 3.15 A / 250 V AC slow-blow
Dosing range 1.0 – 9999.9ml, resolution 0.1ml
Dosing accuracy 0.25 – 2% (depending on selected tube, calibration e.g.)
Dosing speed DP1000 5 – 800ml/min
DP1000F 15 – 2400ml/min
Variable pause between single doses 0.0 – 9.9 seconds
AntiDrop 0 – 90° selectable Auto-reverse angle of pump head
Display 240 x 128 pixels with LED backlighting
Sound pressure level < 79 dB(A)
Pollution severity II
Safety class IP 64
Ambient temperature + 5°C to 45°C (also during transport)
Unit support surface Sturdy support surface
Remote control / footswitch The two footswitch contacts are designed as normally open
contacts (NO).
Interface RS232 RS232 for connection to host and printer
Interface RS485 (option) RS485 for BioLink (communication with lab. instruments)
Stage bar (option) Stainless steel, length = 200 mm, Ø 12 mm
Item number 730000 DosiPump DP1000 Software 2.0 or higher
740000 DosiPump DP1000 Fast Software 2.0 or higher
300 280
210 200
DOSI
PUMP
Power