LumaSense technologies 1313 User manual

BE6007-15
Instruction
Manual
1313 Fermentation
Monitor
BZ6009 – 1313 PC Software

Index
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Index
Index...............................................................................................................................................................................................2
1313 Fermentation Monitor............................................................................................................................................................4
About this Manual..........................................................................................................................................................................6
Chapter 1 Introduction and Specifications ....................................................................................................................................7
1.1 Broad Overview .........................................................................................................................................................8
1.2 Monitor System Description.......................................................................................................................................8
1.2.1 Control Software and PC Interface.....................................................................................................................8
1.2.2 Electro-mechanical and Electro-acoustic Measuring System.............................................................................9
1.2.3 Alarm Relay and Analogue Outputs...................................................................................................................9
1.2.4 Power Supply ...................................................................................................................................................10
1.3 Software Description................................................................................................................................................10
1.3.1 SF6 Version......................................................................................................................................................10
1.4 Acoustic Gas Measurement......................................................................................................................................11
Chapter 2 Installation and Getting Started ..................................................................................................................................15
2.1 Installing the Monitor...............................................................................................................................................16
2.1.1 Operating Environment ....................................................................................................................................16
2.1.2 Transportation Protection.................................................................................................................................16
2.1.3 Current-loop and Alarm Relay Outputs............................................................................................................17
2.1.4 The Gas Sample Line .......................................................................................................................................19
2.1.5 Mains Supply....................................................................................................................................................20
2.1.6 Checking and Changing the Fuses....................................................................................................................20
2.2 The BZ 6009 Software .............................................................................................................................................21
2.2.1 Computer Requirements...................................................................................................................................22
2.2.2 Installing the Software on the PC.....................................................................................................................22
2.2.3 Activate automatic login and Auto start BZ6009 .............................................................................................25
2.3 Connecting the Monitor to the PC............................................................................................................................26
2.4 Starting the Monitor .................................................................................................................................................27
2.5 Starting the Software................................................................................................................................................28
2.5.1 To start the Software ........................................................................................................................................28
2.6 Using the Software.........................................................................................................................................................29
2.6.1 Screen Layout...................................................................................................................................................29
2.6.2 Moving around the Screen................................................................................................................................29
2.6.3 Entering Values ................................................................................................................................................30
2.6.4 Information Screens..........................................................................................................................................30
2.7 Test 1313 & 1309.....................................................................................................................................................31
2.8 Stopping the Software ..............................................................................................................................................32
2.9 Selecting the Correct Serial Port ..............................................................................................................................32
Chapter 3 Configuration..............................................................................................................................................................33
3.1 General Information .................................................................................................................................................34
3.2 Software Configuration ............................................................................................................................................34
3.2.1 BZ 6009 Set-up Screen.....................................................................................................................................34
3.2.2 General Set-up..................................................................................................................................................35
3.2.3 Gas Curves .......................................................................................................................................................37
3.2.4 1309 Sampling..................................................................................................................................................38
3.3 Hardware Configuration...........................................................................................................................................40
3.3.1 0 – 24 mA Set-up..............................................................................................................................................40
3.3.2 Alarm Relays Set-up.........................................................................................................................................43
3.3.3 Adjust Clock.....................................................................................................................................................45
Chapter 4 Calibration ..................................................................................................................................................................47
4.1 Introduction..............................................................................................................................................................48
4.2 Checking and Changing the Particle Filters .............................................................................................................48
4.3 Calibration Gases .....................................................................................................................................................49
4.4 Connecting the Calibration Gas................................................................................................................................50
4.5 Zero Calibration .......................................................................................................................................................51
4.6 Gain Calibration .......................................................................................................................................................53
4.7 Interference Calibration............................................................................................................................................54
4.7.1 Interference Calibration Gases .........................................................................................................................55
4.7.2 Interference Calibration Procedure...................................................................................................................55

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4.7.3 Modified Interference Calibration Procedure...................................................................................................60
Chapter 5 Operation....................................................................................................................................................................61
5.1 About this Chapter....................................................................................................................................................62
5.2 Measurement Monitoring...............................................................................................................................................62
5.2.1 The Measuring Screen Layout..........................................................................................................................62
5.3 Import Data to Excel ................................................................................................................................................67
Chapter 6 1309 Multipoint Sampler............................................................................................................................................69
6.1 About this Chapter....................................................................................................................................................70
6.2 System Description...................................................................................................................................................70
6.3 Setting up the IEEE – 488 Interface.........................................................................................................................71
6.4 Setting up the 1309 IEEE – 488 Address .................................................................................................................71
6.5 Connecting a 1309 to a PC.......................................................................................................................................72
6.6 Connecting a 1309 to a 1313....................................................................................................................................72
6.7 Connecting Temperature Sensors.............................................................................................................................74
6.8 Mains Supply and Fuses...........................................................................................................................................74
Chapter 7 Process Computer Interface........................................................................................................................................75
7.1 About This Chapter ..................................................................................................................................................76
7.2 PCI System Description ...........................................................................................................................................76
7.3 Setting up the Process Control Computer’s RS – 232 serial interface......................................................................77
7.4 Process Computer Interface (PCI) Protocols............................................................................................................77
7.5 Representation of Values in Registers......................................................................................................................78
7.6 COMLI Communication parameters........................................................................................................................79
7.6.1 Register Description.........................................................................................................................................79
7.6.2 The Interface Registers.....................................................................................................................................81
7.7 OPTOMUX Communication parameters.................................................................................................................91
7.7.1 Register Description.........................................................................................................................................91
7.7.2 The Interface Registers.....................................................................................................................................91
7.7.3 Command Structure........................................................................................................................................100
7.7.4 Checksums......................................................................................................................................................101
7.7.5 Response Structure.........................................................................................................................................101
7.7.6 Initialising the Registers.................................................................................................................................103
7.7.7 Command Library ..........................................................................................................................................103
Chapter 8 Troubleshooting and Error Messages .......................................................................................................................109
8.1 Servicing the 1313..................................................................................................................................................110
8.2 Error Monitoring ....................................................................................................................................................110
8.3 Test Hierarchy........................................................................................................................................................111
8.3.1 Tests during Power Up ...................................................................................................................................111
8.3.2 Tests during Power Up and During Start Up of the Measurement System ....................................................112
8.3.3 Tests During a Measurement..........................................................................................................................112
8.3.4 Tests Only During Measurement....................................................................................................................113
8.3.5 Starting the Troubleshooting ..........................................................................................................................113
8.3.6 Communication Problems ..............................................................................................................................113
8.4 Error and Warning Messages .................................................................................................................................114

1313 Fermentation Monitor
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1313
Fermentation Monitor
BZ6009 – 1313 PC Software
December 2010

Safety Considerations
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Safety Considerations
The 1313 Fermentation Monitor is designed and tested to comply with EN 61010-1 (1993)
and IEC 1010 – 1 (1990); Safety requirements for electrical equipment for measurement,
control, and laboratory use. The protection provided by the instrument may be impaired if not
used as specified by Innova AirTech Instruments. To ensure safe operation and retain the
monitor in safe condition, special note should be made of the following:
Explosion Hazard!
TO AVOID THE POSSIBILITY OF AN EXPLOSION, MONITORING OF FLAMMABLE
GASES IN EXPLOSIVE CONCENTRATIONS MUST NEVER BE ATTEMPTED.
Never operate this monitor in potentially explosive environments.
When monitoring potentially flammable or toxic gases it is essential:
•The instrument itself is placed in a well-ventilated area outside the potentially hazardous
zone; and
•That a sufficiently long tube is connected to the Gas Outlet on the back panel so that the
sampled gas is carried away to the open air or to an extraction and/or filtration unit.
Warnings!
•Water condensation in the instrument will damage it severely, and must therefore be
avoided.
•Switch off all equipment before connecting or disconnecting their digital interface. Failure
to do so could damage the equipment.
•Whenever it is likely that the correct function or operating safety of the apparatus has
been impaired, the apparatus must be made inoperative and be secured against unintended
operation.
•Any adjustment, maintenance and repair of the open apparatus under voltage must be
avoided as far as possible and, if unavoidable, must be carried out by trained service
personnel only.
•The instrument contains a lithium battery. Under no circumstances should this battery be
removed by the user as there is a danger of explosion. The lithium battery should only be
removed by an LumaSense Technical Support representative.
Applying Power Before using this apparatus, verify that the correct fuses are installed.
The instrument must always be operated with its chassis at earth potential. Only use power
outlets that can supply an earth to the instrument.
Safety Symbols
Caution: Refer to associated instructions given in this Manual.

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About this Manual
This Reference Manual is for a monitoring system comprising a 1313 Fermentation
Monitor and its associated PC software package BZ 6009. Also included are compre-
hensive descriptions of using the 1313 with the 1309 Multipoint Sampler, with a
Process Control Computer.
The software for 1313 is available in a Windows® XP version as:
BZ6009 for 1313 Fermentation Monitor
Trademarks
MS-Windows are registered trademarks of Microsoft Corporation.
Copyright © 2007, LumaSense Technologies A/S
All rights reserved. No part of this publication may be reproduced or distributed in any form, or
by any means, without prior consent in writing from LumaSense Technologies A/S, Ballerup,
Denmark.

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Chapter 1
Introduction and Specifications
December 2010

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1.1 Broad Overview
The 1313 Fermentation Monitor simultaneously measures the concentration of carbon
dioxide, oxygen and a specified hydrocarbon in a gas flow. The concentration of each
gas is expressed as a volume percentage referenced to dry gas condition. Additionally,
the concentration of the hydrocarbon can be expressed in mg/m3.
The measurement principles are based on photoacoustic spectroscopy and magneto-
acoustic spectroscopy. A full discussion of these principles is contained in section 1.4.
The monitor is designed to be integrated into permanent monitoring systems to provide
fast and accurate measurement results over a wide range of concentrations. Measure-
ment results can be transferred via the monitor’s analogue interfaces to a process-com-
puter or a personal computer (PC).
The monitor comes complete with PC software that allows you to configure and cali-
brate the monitor prior to measuring, display the measurement data graphically while
measurements are being made. The stored data can be recalled at a later stage for closer
examination.
Communication between the PC and the process computer is possible using the
COMLI and OPTOMUX protocols. This communication enables you to access infor-
mation from the 1313 and the 1309; and select the sampling sequence mode.
1.2 Monitor System Description
Fig.1.1 shows a block diagram of the 1313 Fermentation Monitor.
There are four basic blocks:
Control Software and PC Interface
Electro-mechanical and Electro-acoustic Measuring System
Alarm Relay and Analogue Outputs
Power Supply
1.2.1 Control Software and PC Interface
This block contains microprocessor based electronics that take care of interfacing the
monitor to a PC via an RS-232 link, send control data to the measuring system and re-
ceive measurement data from the measuring system.
There are no external controls on the monitor apart from the mains On/Off switch. All
configuration of the monitor is, therefore, done over the serial link using a PC running
the specially developed BZ 6009 software.

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Fig.1.1 Block diagram of the 1313 Fermentation Monitor
However, the monitor does have operating system software contained in read-only
memory. When the monitor is powered up, this internal software checks the system to
ensure it is working correctly and starts the monitor in the configuration it was in just
before the last power shut-down. This configuration is held in an EEPROM and can
only be changed by using the PC software.
This means that once configured and calibrated, you can disconnect the PC and monitor
the actual gas concentrations via the 0 – 24 mA outputs. If you want to record the re-
sults of measurements over time on a data-file, you must have the PC connected, since
the results file is stored on the PC’s disk system.
1.2.2 Electro-mechanical and Electro-acoustic Measuring System
This block is the heart of the monitor. It contains all the mechanics, transducers and
processing electronics necessary to do highly accurate measurement of gas concentra-
tions.
Measurement samples are sucked into the measurement chambers via the Gas Inlet or
the Calibration Gas Inlet, depending upon the position of the moveable link (part of the
particle filter, see Fig.1.1). The internal gas-circuits are arranged such that samples pass
through the measurement chambers in a continuous flow. Exhaust gases are expelled
via the gas outlet. The reference gas used for oxygen measurements (normally ambient
air) is drawn via the Ref. O2Inlet.
1.2.3 Alarm Relay and Analogue Outputs
In this functional block, the electrical equivalent of the measured concentrations in the
sample gases are output as currents (0-24 mA), which can be used to interface to other
monitoring equipment, for example a process computer.

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Alarm output indicating high percentage and low percentage concentration of the
measured gases are also available.
The concentrations that cause the alarms to activate and the value of the current output
with respect to a specific concentration are user definable.
1.2.4 Power Supply
The power supply included in the 1313 can be directly connected to a wide range of
single phase mains supplies and still be able to produce the stable voltages required to
keep the monitor within calibration.
The fan unit fixed to the front panel provides forced ventilation to keep the power sup-
ply, electronics and mechanical parts in the system cool. The fan intake and the rear
panel ventilation slots must be kept clear at all times.
1.3 Software Description
The BZ 6009 software is menu driven specifically written to control the 1313 monitor.
The software allows you to change the operational parameters and see the results of
measurements on the PC screen.
The software can also be used to control up to three 1309 Multipoint Samplers. In-
cluding these instruments in your system allows up to 36 different sample points to be
measured by the 1313.
The software has the capability of being controlled remotely by a process control com-
puter using a process control interface based upon an RS–232 serial link. You can thus
integrate your 1313 measuring system into a much larger monitoring and control envi-
ronment.
You can also use the PC’s filing system to save a series of measurements for later recall
either by the BZ 6009 software or any other PC software that can interpret a comma
delimited file, for example certain spreadsheet programs.
Note though that the save-to-disk process is not a dump of data from the 1313 to the
PC, but a process in which the data file on the PC is updated continuously.
Therefore, to use the PC to log data, it must be constantly attached to the 1313. This
contrasts with the analogue outputs, which are always active during measurement,
whether the PC is connected or not.
As well as providing control over the measurement configuration of the monitor, the
software also has comprehensive calibration procedures that allow you to calibrate the
monitor against known reference gas concentrations.
1.3.1 SF6 Version
A special version of the User Software is available for the 3429 and 1313 modified for
SF6 as the BZ6010 software. Throughout this Manual for the 1313 Fermentation

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Monitor, BZ6009 should be substituted with BZ6010, and CHx should be substituted
with SF6.
1.4 Acoustic Gas Measurement
Acoustic techniques for gas measurement are based on the principle that if energy is
applied to a gas it will expand. Expansion causes an increase in pressure. If the applied
energy is delivered in pulses, the pressure increase will be intermittent, resulting in a
pressure fluctuation. The fluctuating pressure will manifest itself as a sound wave,
which can be “listened to” by a microphone. The microphone will give an electrical
output proportional to the intensity of the pressure fluctuations, which in turn are pro-
portional to the concentration of the gas present.
The gases of major interest here are hydrocarbons, carbon dioxide and oxygen. Hydro-
carbons and carbon dioxide readily absorb infra-red light, so this can be used as the ap-
plied energy source to give pressure fluctuations. Oxygen is not affected by infra-red
light in the same way, but is highly susceptible to magnetic “energy”, so this is used to
give pressure fluctuations.
When using light as a stimulant to produce the sound wave, the technique is called
photoacoustic spectroscopy (PAS). When the stimulant is magnetism, the term magne-
toacoustic spectroscopy is used.
A further gas property that is used in PAS is that the wavelengths of infra-red light that
each gas type absorbs is different. To benefit from this property, a gas sample is irradi-
ated with infra-red light chosen to contain the wavelengths best absorbed by the gas.
These wavelengths are then pulsed at different rates to produce different sound waves.
By correctly filtering the microphone signal, the concentration of each type of gas can
be measured, even when they are part of the same sample.
Photoacoustic Spectroscopy in the 1313
The 1313 has four major components in its PAS based measuring system (see also
Fig.1.2):
•A stable infra-red source
•Mechanical pulsation system
•Measurement chamber
•Microphone
The infra-red source provides infra-red light with a broadband spectrum. Unless the
amount of light and spectral content is kept constant for every measurement, it is not
possible to determine the absolute gas concentration referred to a calibration gas.
Therefore, the infra-red source is kept stable by electronic feedback systems, thus en-
suring the quantity and spectral content remains constant.

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The infra-red point source is located at the focus of a concave mirror. This is used to
provide a beam of infra-red light, which is focused through the chopper wheel into the
measurement chamber.
The chopper wheel is driven by an electric motor running at a constant speed. The
chopper wheel consists of three sets of concentric holes. The full infra-red beam from
the mirror is incident on one side of the chopper wheel. After passing through the holes
in the wheel, the beam is effectively divided into three beams pulsating at different fre-
quencies. The pulsation frequency is determined by the whole spacing and motor
speed.
Fig.1.2 Schematic representation of the measurement system
In terms of rise time and ambient noise suppression, a high pulsation frequency is de-
sirable. However, one must also consider that high frequencies also result in less time
for the applied energy to affect the gas, giving smaller output signals from the micro-
phone. The frequencies used in the 1313 are chosen to give a balance between the sig-
nal level and noise suppression requirements.
Each light beam now passes through an optical filter located on the measurement
chamber. The focusing of each pulsating beam is arranged such that it only passes
through one filter. Each filter only allows one specific wavelength of infra-red light to
pass. Thus the beams entering the measurement chamber from the filters will be nearly
monochromatic, but still pulsating at their specific frequency.
The filter wavelengths are optimised to match the absorption spectra of hydrocarbons,
carbon dioxide and water vapour.

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The filter associated with hydrocarbons is centred on 3.4 µm (2950 cm-1), the filter as-
sociated with carbon dioxide is centred on 4.3 µm (2350 cm-1), and the filter associated
with water vapour is centred on 5.3 µm (1900 cm-1). Water vapour is measured to com-
pensate for the humidity of the sampled gas.
In the measurement chamber the three beams, which differ in wavelength and pulsation
frequency, each excite their respective gas (if present). The absorption of the incident
light will cause the gas to expand and contract at a frequency equal to the pulsation fre-
quency. The resulting sound waves will fill the measurement chamber and be propa-
gated further on in the gas circuit. A microphone is mounted close to the measurement
chamber such that it can “hear” the gases.
The output of the microphone will be a complex waveform made up of the individual
intensities and frequencies associated with each gas. This waveform is amplified and
converted into a digital signal. A digital signal processor then extracts the individual
components of the combined signal. The three resulting digital waveforms will be pro-
portional to the concentration of the gas in the measurement chamber.
By calibrating the system with known concentrations of hydrocarbon, carbon dioxide,
oxygen and water, the absolute concentration values can be calculated.
Advantages of Photoacoustic Spectroscopy
Photoacoustic gas measurement is based on the same principles as conventional infra-
red base gas analysers, namely the ability to absorb infra-red light. However, there are
some important differences between PAS and these conventional techniques.
In the standard IR-analyser, the energy absorbed by the gas sample is measured indi-
rectly by measuring the transmission through the measurement chamber and comparing
it to that transmitted through a reference cell. With PAS the amount of infra-red ab-
sorbed is measured directly by measuring the sound energy emitted on the absorption
of light. This means PAS is highly accurate with very little instability. For example,
zero point drift is small as zero is always reached when no gas is present – with no gas
present there is virtually no acoustic signal.
Furthermore, with PAS all gases and vapours can be monitored simultaneously in a
single measurement chamber when the correct filtering of the microphone signal is
used. The use of a microphone is also a major difference over traditional IR-analysers
that use photo-sensors to detect the signal. In the 1313 an optimised condenser micro-
phone is used as the detector.
LUMASENSE have many years experience with such microphones and the processing
of microphone signals. The microphones and associated processing electronics used in
the 1313 are of an exceptionally high quality. They give a high degree of stability and
reliability, which means calibration is rarely required.

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Magnetoacoustic Spectroscopy in the 1313
The 1313 has two major components in its Magnetoacoustic Spectroscopy based meas-
uring system (see also Fig.1.2):
•A switched magnetic field
•Microphone
In PAS, the energy source to excite the gas is infra-red light. In magneto acoustic spec-
troscopy, the source is a magnetic field. An alternating magnetic field applied to a gas
containing oxygen will cause the oxygen to expand and contract, causing sound waves,
as in PAS. Unlike PAS however, elaborate filtering techniques do not need to be ap-
plied to the excitation energy since oxygen is the only paramagnetic gas that will be
measured by the 1313.
There is no actual measurement chamber in the oxygen measuring circuit. Instead, the
gas sample is subjected to the magnetic field just after it has passed the PAS system.
Because the frequency of the magnetic field is chosen to be different from those used in
the PAS system, the same microphone can be used to detect the sound waves resulting
from the oxygen expansion and contraction.
In theory, the absolute value of the oxygen in the sample gas can be directly measured
in this way. However, to obtain the same degree of accuracy in measuring gas concen-
trations as that obtained using PAS, the measured signal is compared to a reference sig-
nal.
The reference signal is generated by passing a reference gas containing a known con-
centration of oxygen through the same magnetic field as the sample gas. The reference
gas will also produce a sound wave directly proportional to its oxygen content. This
sound wave is measured by a second microphone solely associated with the reference
gas circuit. The output of this microphone is passed into a similar digital processing
system as the sample microphone.
When the processing system has extracted the information about the oxygen concentra-
tion in the sample and the reference, the two measurements can be compared to the
stated concentration of the reference gas. Any difference between the reference gas’s
measured and stated concentration will be used as an offset to correctly determine the
absolute concentration in the sample gas.
In normal practice ambient air is used as the reference gas for the oxygen measurement
as it contains a stable amount of oxygen – 20.95 % referenced to dry gas condition.

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Chapter 2
Installation and Getting Started
December 2010

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2.1 Installing the Monitor
The 1313 monitor is designed to fit in a 19 inch rack. The front panel has a serial-link
socket for the PC interface and a gas inlet for calibration gases. The mains input, ana-
logue output, alarm relay output, sample gas inlet and outlet for expelled gas samples
are located on the rear panel.
2.1.1 Operating Environment
The following environmental conditions must be observed:
•Safe ambient operating temperatures range from 10°C to 40°C (50°F to 104°F).
•Humidity should not exceed 90% RH non-condensing at 40°C.
•Vibrations must be avoided as these can upset the quality of the measurement
data.
•Keep the front panel fan intake and rear panel ventilation slots unobstructed at
all times.
Warning! The monitor is not intended for use in explosive environments and should be
kept well away from flammable gases and liquids. Avoid excessive heat, dust and di-
rect sunlight.
2.1.2 Transportation Protection
The monitor is equipped with two special locking-screws for prevention of accidental
damage to the measurement transducers during transportation. These protection screws
must always be loosened before the monitor is used.
Loosening the transport protection screws should be carried out by a qualified techni-
cian.
Caution! Be careful not to touch the chopper wheel when loosening the protection
screws!
To loosen the transportation screws:
1. Ensure that the monitor is disconnected from the mains power supply.
2. Remove the ten pozidrive screws that secure the top plate in position and lift
this plate off.
3. Locate the protection screws (see Fig.2.1).
4. Loosen the screws by rotating them anti-clockwise until they cannot be turned
any more.
5. Replace the top plate and re-secure it in place.

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Fig.2.1 The transportation screws (indicated by arrows)
Note: it is not necessary to use the transport protection during local movement of the
monitor. However, the protection screws should be tightened again before the monitor
is moved over longer distances.
2.1.3 Current-loop and Alarm Relay Outputs
The 1313 has analogue outputs giving a current (between 0 mA and 24 mA) equivalent
to the gas concentration, and two isolated single-pole relay contacts giving over- and
under- concentration alarms.
The analogue output connector is located on the rear panel of the monitor (see Fig.2.2).
The connector has sixteen individual screw terminals, which are assigned as shown in
Table 2.1.
The higher numbered terminal in a pair is positive. The three analogue outputs are gal-
vanically isolated from each other and from the chassis.
The ground connections are all connected together internally and are provided as an an-
choring point for any screens that your data-cables may have. To comply with EN
50082–2 (Immunity to RF fields), it is necessary to use screened cables and connect the
screens to the chassis terminals.
To connect wires to the analogue output connector:
1. Choose the desired screw terminal.
2. Using a flat-blade screwdriver, loosen the screw immediately above the desired
terminal number.
3. Insert the wire into the terminal above the loosened screw.
4. Tighten the screw again, ensuring that the wire is now gripped firmly in the
terminal.

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5. Repeat steps 1 to 4 to connect all the necessary wires for the analogue outputs
you need.
Fig.2.2 The Current Loop and Alarm Relay connector
Screw Terminal
Number Assignment
1 & 2 Low limit relay
3 & 4 High limit relay
5 Chassis
6 & 7 Hydrocarbon values
8 Chassis
9 & 10 Carbon dioxide values
11 Chassis
12 & 13 Oxygen values
14 Chassis
15 & 16 Unassigned
Table 2.1 Assignments for the analogue output socket
The range of the signals that come from the current outputs are user-scalable (the us-
able range is between 0 and 24 mA). This enables you to select a range that best suits
the concentrations of the gas you are measuring, and obtain the optimum resolution for
the process computer. These definable values are set via the PC software. Definition is
described in section 3.3.1.

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2.1.4 The Gas Sample Line
The gas to be sampled (measured) is drawn from the sample point via a sample line at a
flow rate of 130 ml/min. Various types of sample lines are available depending on the
individual applications. The sample line tubing between the sample point and the
monitor should be kept as short as possible to assist response times. If you are unsure
about the type of sample line for your application, contact your LUMASENSE repre-
sentative for advice before installing the system.
The pressure difference between the monitor’s gas inlet and the ambient must be in the
range from – 5.3 kPa to +2 kPa. When a positive higher pressure exists, a surplus path
to the ambient can be established.
A 3.3 kPA pressure drop across the sampling line used corresponds to the following
sample tube lengths:
•Inner diameter of 1 mm gives a length of 2.5 m
•Inner diameter of 2 mm gives a length of 40 m
Note: You must use a filter in the gas-line immediately before the gas-line enters the
monitor. The UD 5057 in-line filter (optional accessory) is ideal for this purpose. For
details of changing the filter element in the UD 5057, please refer to section 4.2.
The gas inlet for the sample line is located on the rear panel of the monitor. The stan-
dard nozzle installed accepts PTFE tubing with a 4 mm external diameter. LumaSense
recommends that the internal diameter of the tube is 2 mm. Be sure to press the tubing
as far into the nozzle as possible to ensure a gas tight seal.
Moreover, to help humidify or de-humidify the sample gas, LumaSense also recom-
mends that at least a 1 m length of Nafion®tubing be incorporated into the sample line.
If the dew point of the gas sample is comparable to the temperature in the monitor,
Nafion tubing can be used to remove water and eliminate the risk of condensation in-
side the monitor. If the gas sample is completely dry, it is advisable to humidify the
sample by using Nafion tubing as this will improve the precision of the CO2measure-
ment.
Nafion is impermeable to O2, CO2, CH4and most other hydrocarbons, whereas alcohols
and other gases will completely or partly penetrate through the material. Contact an
LUMASENSE Technical Support representative for advice on whether or not to use
Nafion when monitoring a particular gas.
You should protect the Nafion tubing against the effects of ultra-violet light, as this re-
duces the tubes lifetime. Nafion tubing should be replaced at regular intervals to ensure
that it still behaves according to specification.
After entering the monitor, every gas sample immediately passes through the particle
filter located on the front panel. To inspect the condition of the particle filter, please see
section 4.2.

Chapter 2
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BE6007-16 1313 Fermentation Monitor LumaSense Technologies A/S
Instruction Manual Page 20 of 116
The standard nozzles can be removed to enable other adapters or connectors to be fit-
ted. When the nozzle is removed, the gas inlet has an 1/8th inch British Standard Par-
allel (BSP) thread. It is important that any other connectors used here have exactly the
same thread.
2.1.5 Mains Supply
Warning. The monitor must be used with protective earthing.
The monitor operates in the AC voltage range from 85 – 264V on a single phase sup-
ply, 50 Hz – 60 Hz.
The power cable is connected to a socket on the rear panel. The on-off switch is located
immediately above this socket.
2.1.6 Checking and Changing the Fuses
On delivery, two 20 mm T1.6 A slow-blow fuses are installed in the monitor – one in
the live and one in the neutral mains feed. If you want the monitor to conform to CSA-
approved standards, you must use the additional fuse holders (JS 0075) supplied with
the instrument, but using 32 mm T1.6 A slow-blow fuses instead. When replacing fuses
always ensure that the correct fuses are used.
To check and change the fuses:
1. Remove the power cable from the mains socket.
2. Insert a small screwdriver under the top edge of the plate covering this socket
and use it to lever the plate downwards. See Fig.2.3.
Note: The cover cannot be removed when the power cable is connected to the monitor.
Fig.2.3 Opening the fuse-holder cover