MGI MGIDL-T7RS User manual

DNB Loader
Part No.: H-020-000137-00
MGIDL-T7RS
[email protected]E-mail:
Building 24, Stage 3.1, BioLake Accelerator, No.388, 2nd Gaoxin Road, East Lake
High-Tech Development Zone, 430075, Wuhan, P.R.China
www.mgi-tech.comWebsite:
Address:
Wuhan MGI Tech Co., Ltd.
Research Use
Only
Version: 3.0

About the user manual
This user manual is applicable to MGIDL-T7RS DNB Loader. The
manual version is 3.0 and the software version is V1.
This manual and the information contained within are proprietary to
Wuhan MGI Tech Co., Ltd. (hereinafter called MGI), and are intended
solely for the contractual use of its customer in connection with the
use of the product described herein and for no other purpose. Any
person or organization can not entirely or partially reprint, copy,
revise, distribute or disclose to others the manual without the prior
written consent of MGI. Any unauthorized person should not use
this manual.
MGI does not make any promise of this manual, including (but not
limited to) any commercial of special purpose and any reasonable
implied guarantee. MGI has taken measures to guarantee the
correctness of this manual. However, MGI is not responsible for any
missing parts in the manual, and reserves the right to revise the
manual and the device, so as to improve the reliability, performance
or design.
Figures in this manual are all illustrations. The contents might
be slightly different from the device, please refer to the device
purchased.
Microsoft®and Windows®are trademarks or registered trademarks
of Microsoft Corporation in the U.S. and/or other countries.
Intel®and Intel CoreTM are trademarks of Intel Corporation or its
subsidiaries in the U.S. and/or other countries. Other names and
brands mentioned in this manual may be claimed as the property of
others.
©
2020-2022Wuhan MGI Tech Co., Ltd. All rights reserved.

Revision history
Date Version
Revision
May 24, 2022 3.0
February 22, 2021 2.0
Initial release December 30, 2020 1.0

I
Contents
Safety 1
Conventions 2
General safety 2
Electrical safety 3
FCC statement 4
IC statement 5
Mechanical safety 5
Components safety 5
Biological safety 6
Symbols 6
Device 6
Label 7
Packaging 9
Device overview 11
Intended use 12
Working principle 12
Structural composition 12
Front view 13
Back view 15
Flow cell stage A 16
Flow cell 17
Plate tray unit 17
Control software 18
Self-test interface 18
Main interface 19
Log interface 21
Settings interface 21
Maintenance interface 22

Contents
II
Shutdown or restart interface 22
About interface 22
Getting started 23
Site requirements 24
Network requirements 24
Operating environments of the computer 24
Software security 25
Data and device ports 25
Inspecting the device 25
Preparing the power supply 26
Installing the device 26
Powering on or off the device 27
Powering on the device 27
Powering off the device 27
Preparing materials for post wash 27
Material list 27
Preparing the post-loading plate 28
Preparing materials for loading 28
Loading 29
Workflow 30
Preparing sample libraries and materials 30
Performing a pre-wash (optional) 30
Loading consumables 31
Performing a load 34
Performing a post-wash 35
Maintaining the device 37
Maintaining the main unit 38
Daily maintenance 38

Contents
III
Weekly cleaning 38
Monthly cleaning 38
Service plan 39
Maintaining the power supply 39
Maintaining the flow cell stage 39
Tools and solutions 39
Cleaning the flow cell stage 39
Replacing the sealing ring 40
Maintaining the software 41
Troubleshooting 41
Storage and transportation 43
Disposal of the device 44
Specifications 45
Compliance information 47
Manufacturer information 49
European representative information 51
Index 53

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1
Safety
This chapter describes basic safety information about the device. Carefully
read and understand the information before use to ensure correct operations,
best performance, and personnel safety. Keep this manual at hand for
reference at any time.

Safety
2
Conventions
The following table describes conventions that are used in the
manual:
Item Description
Indicates that the operator should operate the device
by following the instructions. Otherwise, it might result
in personal injury.
Indicates that the operator should operate the device
by following the instructions. Otherwise, it might
result in device malfunction, damage or inaccurate
experiment result.
Indicates that the operator should pay special attention
to the note information, and operate the device by
following the instructions.
Indicates biological risk. The operator should operate
the device by following the instructions.
Boldface Indicates the printings and on-screen characters on the
device.
General safety
zEnsure that the device is operated under the conditions specified in this
manual. Otherwise, it might result in device damage, incorrect experiment
results, or even cause personal injury.
zEnsure that the components of the device are completely installed before
operation. Otherwise, it might result in personal injury.
zMaintain the device by following the instructions in
Maintaining the device
on Page 37 to ensure best performance. Otherwise, it might result in
device malfunction or even personal injury.
zOnly the technical support authorized by the manufacturer or the qualified
and trained personnel can unpack, install, move, and maintain the device.
Incorrect operation might cause inaccurate experiment results or damage to
the device.

Electrical safety
3
zDo not move the device after the technical support have installed
and debugged the device. Incorrect operation will result in inaccurate
experiment result. If you require to re-position the device, contact the
technical support.
zOnly trained professionals such as doctors, technicians, or laboratory
assistants can operate the device.
zDo not operate the device in the presence of flammable or explosive
liquids, vapors or gases. Otherwise, it might result in device malfunction.
zDo not disconnect the power cord under the power-on status.
zDo not place tubes or plate on the device. Liquids seeping into the device
might cause malfunction.
zDo not reuse the disposable items.
zOnly the peripheral devices and consumables specified by the manufacturer
can be used.
zIf malfunctions related to fluidics lines (for example, bubble) occur during
an experiment, solve the problems before you restart an experiment.
zIf you have maintenance questions that are not mentioned in this manual,
consult the technical support.
zOnly the components provided by the manufacturer can be used for
device maintenance. Unapproved components might damage the device or
degrade performance.
zThe device is verified before delivery. If serious deviation occurs during use,
contact the technical support for calibration.
Electrical safety
zBefore initial use of the device, assess the electromagnetic environment in
which the device will be used.
zDo not use the device in close proximity to the sources of strong
electromagnetic fields, such as unshielded sources of radiated emissions.
Radiated signals can reduce the accuracy of the results.
zEnsure that the device is properly grounded, and the grounding resistance is
less than 4 Ω . Failure to do so might result in inaccurate experiment results
or even electric shock.
zDo not remove the device cover and expose the inner component outside.
Otherwise, it might cause electric shock.

Safety
4
zOnly the power cord supplied by the manufacturer can be used.
zPrepare the laboratory and power supply according to
Getting started on
Page 23
.
zEnsure that the input voltage meets the device requirement.
FCC statement
This device complies with part 15 of the FCC Rules. Operation is
subject to the following two conditions:
1. This device may not cause harmful interference, and
2. This device must accept any interference received, including
interference that may cause undesired operation.
Any Changes or modifications not expressly approved by the
party responsible for compliance could void the user’s authority to
operate the equipment.
This equipment should be installed and operated with a minimum
distance of 25cm between the radiator and your body.
This equipment has been tested and found to comply with the limits
for a Class B digital device, pursuant to Part 15 of the FCC Rules.
These limits are designed to provide reasonable protection against
harmful interference in a residential installation. This equipment
generates uses and can radiate radio frequency energy and, if not
installed and used in accordance with the instructions, may cause
harmful interference to radio communications. However, there is no
guarantee that interference will not occur in a particular installation.
If this equipment does cause harmful interference to radio or
television reception, which can be determined by turning the
equipment off and on, the user is encouraged to try to correct the
interference by one or more of the following measures:
¾Reorient or relocate the receiving antenna.
¾Increase the separation between the equipment and receiver.
¾Connect the equipment into an outlet on a circuit different from that to
which the receiver is connected.
¾Consult the dealer or an experienced radio/TV technician for help.

IC statement
5
IC statement
This device complies with Industry Canada’s licence-exempt RSSs.
Operation is subject to the following two conditions:
1. This device may not cause interference; and
2. This device must accept any interference, including interference
that may cause undesired operation of the device.
The distance between user and products should be no less than 20 cm.
Le pr
é
sent appareil est conforme aux CNR d’Industrie Canada
applicables aux appareils radio exempts de licence. L’exploitation est
autoris
é
e aux deux conditions suivantes:
1. l’appareil ne doit pas produire de brouillage, et
2. l’utilisateur de l’appareil doit accepter tout brouillage
radio
é
lectrique subi, m
ê
me si le brouillage est susceptible d’en
compromettre le fonctionnement.
La distance entre l’utilisateur et de produits ne devrait pas
ê
tre
inf
é
rieure
à
20 cm.
Industry Canada ICES-003 Compliance:CAN ICES-3(B)/NMB-3(B)
Mechanical safety
To avoid falling off and personal injury, place the device on a level surface
and ensure that the device cannot be easily moved.
Components safety
Only the software that have been provided by the manufacturer can be
installed and used on the computer. Unknown software might interfere
with normal device functions, or even cause data loss. If you need to install
antivirus software, contact the technical support in advance.
zIf the fuse blew, replace the fuse with the specified type. For details,
contact the technical support.

Safety
6
zDo not uninstall the control software by yourself. If any problem occurs
during software operation, contact the technical support.
zEnsure that the peripheral devices meet the IEC/EN 60950-1 standards.
Biological safety
zChemicals in reagents and waste might cause personal injury through
contact with the skin, eyes, and mucosa. Follow the safety standards of
your laboratory and wear protective equipment (such as laboratory coat,
disposable bouffant cap, protective glasses, mask, gloves, and shoe covers).
zIf you accidentally splash the reagent on the skin or into eyes, immediately
flush the affected area with large amounts of water and get medical aid
immediately.
zUse and store the reagents according to the reagent kit user manual. Failure
to do so might negate the reagent effects and cause inaccurate results.
zCheck the expiration date of all reagents before use. Do not use expired
reagents.
zWhen disposing of the expired reagents, waste liquids, waste samples, and
consumables, comply with local regulations.
Symbols
Device
The following table describes symbols on the device:
Symbol Name Description
"ON" (power) Indicates connection to the
mains supply.
"OFF" (power) Indicates disconnection from
the mains supply.
General
warning sign Signifies a general warning.
Warning;
biological
hazard
Warns of a hazard from a
biological hazard.

Symbols
7
Symbol Name Description
Warning;
dangerous
voltage
Indicates hazards arising from
dangerous voltages.
Protective earth
Indicates the terminal of a
protective earth (ground)
electrode.
F10AL250V Fuse
specification Indicates the fuse specification.
R
FI
D
RFID (Radio
frequency
identification)
reader
indication
Identifies the ID of the item
placed near the area.
SWITCH Power switch Powers on or off the device.
SOCKET Power port Connects to the mains supply.
LAN RJ45 network
port
Connects the network of the
computer and server.
USB 2.0 port Connects to the USB device.
USB 3.0 port Connects to the USB device.
Label
The following table describes symbols on the label:
Symbol Name Description
Research Use
Only
/
Indicates a device that is for
research use only, and cannot
be used for clinical diagnosis.
Manufacturer Indicates the medical device
manufacturer.
EC REP
Authorized
representative
in the European
Community
Indicates the Authorized
representative in the European
Community.

Safety
8
Symbol Name Description
Date of
manufacture
Indicates the date when
the medical device was
manufactured.
SN Serial number
Indicates the manufacturer’s
serial number so that a
specific medical device can be
identified.
CE Mark of
Conformity
Indicates that this device
conforms with the specified
Council Directive.
RoHS mark
Indicates that this device meets
the requirements of Directive
2011/65/EU on the restriction
of the use of certain hazardous
substances in electrical and
electronic equipment.
E115163
NRTL
Listing and
Certification
Mark
Used to designate conformance
to nationally recognized
product safety standards. The
Mark bears the name and/or
logo of the testing laboratory,
product category, safety
standard to which conformity is
assessed and a control number.
Consult
instructions for
use
Indicates the need for the user
to consult the instructions for
use.
WEEE symbol
Indicates that waste electrical
and electronic equipment
must not be disposed of as
unsorted municipal waste
and must be collected
separately. Please contact
an authorized representative
of the manufacturer for
information concerning the
decommissioning of your
equipment.

Symbols
9
Packaging
The following table describes symbols on the packaging or on the
label of the packaging:
Symbol Name Description
This way up
Indicates the correct upright
position of the transport
package for transport and/ or
storage.
Fragile, handle
with care
Indicates a medical device that
can be broken or damaged if
not handled carefully.
Keep dry
Indicates a medical device that
needs to be protected from
moisture.
Do not stack
Indicates that stacking of
the transport package is not
allowed and no load shall
be placed on the transport
package.
Do not roll
Indicates that the transport
package shall not be rolled or
turned over but shall remain in
the upright position.
Temperature
limit
Indicates the temperature
limits to which the medical
device can be safely exposed.
Humidity
limitation
Indicates the range of humidity
to which the medical device
can be safely exposed.
Atmospheric
pressure
limitation
Indicates the range of
atmospheric pressure to which
the medical device can be
safely exposed.

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11
Device overview
This chapter describes the intended use, working principle, and structural
composition of the device.

Device overview
12
Intended use
The device is used to prepare sequencing flow cells by loading the
pre-prepared DNBs (DNA Nanoball) and/or reagent to a flow cell.
This device is intended only for scientific research and should not be used
for clinical diagnosis.
Working principle
The device loads the sample libraries and/or reagent to a sequencing
flow cell through defined and optimized vacuum procedures.
Structural composition
The device consists of the touch screen monitor, PCR board
assembly, Y-Z motion stage, flow cell stage, syringe pump, vacuum
pump, and RFID reader.
The following table describes functions of each component:
Component Description
Touch screen
monitor
Displays the information and performs on-screen
operation.
PCR board assembly Controls the system, drives the components,
collects status, and feeds back to the system.
Y-Z motion stage Switches among different reagents.
Flow cell stage Connects the fluidics line and the flow cells and
controls the flow cell stage temperature.
Syringe pump Aspirates the reagent to the flow cell and
discharges the waste to the post-loading plate.
Vacuum pump Firmly attaches the flow cell to the inlet and
outlet of the sealing rings, to avoid liquid leakage.
RFID reader Identifies the ID of an item.

Structural composition
13
Front view
1
2
3
4
5
6
8
7
9
10
11
12
13
R FI D
Figure 1 Front view
No. Name Description
1Touch screen monitor Facilitates the on-screen operation and
displays information.
2Flow cell compartment Holds the flow cell.
3Flow cell compartment
door
Pushes it right or left to open or close
the flow cell compartment door.
4Loading compartment Places the post-loading plate and load
the liquid.