Olympus IX2-DICD User manual

INSTRUCTIONS
IX2-DICD
This instruction manual is for the Olympus DIC Condenser Model IX2-DICD. To ensure the safety, obtain
optimum performance and to familiarize yourself fully with the use of this equipment, we recommend
that you study this manual thoroughly before operating the system. Retain this instruction manual in an
easily accessible place near the work desk for future reference.
A X 7 3 5 9
DIC CONDENSER
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IMPORTANT — Be sure to read this section for safe use of the equipment. — 1-2
IX2-DICD
CONTENTS
3-1 Brightfield Observation ..........................................................................................................................................................................................................................14
3-2 DIC Observation .................................................................................................................................................................................................................................... 14-17
1 NOMENCLATURE 3-4
4 TROUBLESHOOTING GUIDE 18
2 ASSEMBLY 5-13
3 OBSERVATION METHODS 14-17
5 SPECIFICATIONS 19
1Cross-Nicol Adjustment 2Observation Procedure
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1
IMPORTANT
· This product is a slim, single type condenser that can be combined with a top lens matching the desired observation (dry,
oil-immersed or water-immersed).
· The water-immersed top lens (IX2-TLW) features an NA of 0.9, WD or 3.7 mm and manipulator access angle of 40°. In high-
resolution DIC (Differential Interference Contrast) observation, these features allow injection operation to be performed
simultaneously.
1Getting Ready
1. This manual pertains only to the DIC condenser. Please also read the instruction manual for the IX2 series (IX81/IX71/IX51)
or IX series (IX70/IX50) microscope to understand the comprehensive operating instructions of the microscope system.
2. The DIC condenser is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact.
3. Be careful not to leave dirt or fingerprints on the lens components.
4. The stopper mechanisms provided for the functions indicate the limits of motion. Never attempt to go beyond a stopper
by applying excessive force.
5. When the PlanApoN60X, UPlanSApo60XO, PlanApo60XO3 and U-DICTHC are combined, (using the U-DIC60HC or U-
DPO60HC DIC prism), color irregularities may be noticeable with certain specimens. Due to this, it is recommended to use
a photo lens (PE3.3X or with a higher magnification) in photomicrography.
6. Do not tighten the clamping screws of the DIC prism and slider too much.
2Maintenance and Storage
1. To clean the lenses and other glass components, simply blow dirty away using a commercially available blower and wipe
gently using a piece of cleaning paper (or clean gauze). If a lens is stained with fingerprints or oil smudges, wipe it gauze
slightly moistened with commercially available absolute alcohol.
Since the absolute alcohol is highly flammable, it must be handled carefully. Be sure to keep it away from open
flames or potential sources of electrical sparks -- for example, electrical equipment that is being switched on or off.
Also remember to always use it only in a well-ventilated room.
2. Never attempt to disassemble any part of the equipment, for this may degrade the performance.
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IX2-DICD
2
If the condenser is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the
equipment may also be damaged. Always use the system as outlined in this instruction manual.
The following symbols are used to set off text in this instruction manual.
: Indicates that failure to follow the instructions in the warning could result in bodily harm to the
user and/or damage to equipment (including objects in the vicinity of the equipment).
# : Indicates that failure to follow the instructions could result in damage to equipment.
} : Indicates commentary (for ease of operation and maintenance).
3Caution
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3
NOMENCLATURE
DIC Condenser
IX2-DICD
Polarizer clamping knob
Polarizer rotation dial
Aperture iris diaphragm
lever
DIC prism slider
(x 2 provided)
Knob
(Detachable)
Condenser height fine
adjustment knob
Polarizer engagement knob
Slider clamping knob
DIC prism slider insertion slot
Top lens mount
DIC prism/slider clamping knob
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IX2-DICD
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Dry Top Lens
U-TLD Oil-Immersed Top Lens U-TLO Water-Immersed Top Lens
IX2-TLW
DIC Prism
See the applicable objective
list (pages 7 to 12).
DIC Slider
U-DICT
U-DICTS
U-DICTHC
U-DICTHR
Prism position selector lever*
Pushed in: BFP1
Pulled out: NORMAL
Prism movement knob
*This lever is not provided for the U-DICT.
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5
ASSEMBLY
2-1 Attaching the Top Lens
}According to the desired observation, attach the dry (U-TLD), oil-immersed
(U-TLO) or water-immersed (IX2-TLW) top lens to the DIC condenser.
1. The U-TLD or U-TLO top lens @ can be attached by screwing it clockwise
into the top lens mount ².
2. To attach the IX2-TLW top lens, turn the top lens mount ² counterclock-
wise to remove it and screw in the top lens into the position of the top lens
mount.
Precautions on Oil-Immersed Top Lens
#When a slide glass with thickness of 1.2 mm or more is used, the field
iris diaphragm image may not be observed.
#Use an objective with 20X or higher power.
Fig. 1
²
@
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IX2-DICD
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Fig. 2
Fig. 3
2-2 Attaching the DIC Prism
}In the DIC prism slider, mount the DIC prism matching the objective, DIC
slider and top lens in use. (For the combination list, see pages 7 to 12.)
Procedure (Figs. 2 & 3)
#To ensure the optical performance, do not tighten the clamping screws
@ and | too much.
1. Loosen the two clamping screws @ by inserting the DIC prism/slider
clamping knob provided with the DIC condenser into each of them.
2. Align the positioning pin of the DIC prism ² with the groove on the slider,
drop the DIC prism into the slider and tighten the two clamping screws @.
3. Turn the DIC prism slider ³ by 180° and insert it into the condenser. Tighten
the slider clamping screw | using the DIC prism/slider clamping knob.
}If the knob ƒ on the DIC slider comes in the way, remove the knob by
turning it counterclockwise.
²
@
|
³
ƒ
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Combinations of Objectives, DIC Sliders, Top Lenses and DIC Prisms
To be used in the BFP1 position of the DIC slider.
DIC Slider U-DICT U-DICTS (Shift type)
Top Lens U-TLD/IX2-TLW U-TLO U-TLD/IX2-TLW U-TLO
UPlanFLN 10X2 U-DIC10 --- U-DIC10 ---
20X U-DIC20 U-ODIC20 U-DIC20 U-ODIC20
40X U-DIC40 U-ODIC40 U-DIC40 U-ODIC40
40XO --- --- U-DIC40 U-ODIC40
60X U-DIC60 U-ODIC60 U-DIC60 U-ODIC60
60XOI
UPlanSApo 10X2 U-DIC10 --- U-DIC10 ---
20X U-DIC20 U-ODIC20 U-DIC20 U-ODIC20
20XO
40X2 U-DIC40 U-ODIC40 U-DIC40 U-ODIC40
60XO --- --- U-DIC60 U-ODIC60
60XW U-DIC60 U-ODIC60 U-DIC60 U-ODIC60
100XO U-DIC100 U-ODIC100 U-DIC100 U-ODIC100
PlanApoN 60XO --- --- U-DIC60 U-ODIC60
UIS2 Series
100XO2 U-DIC100 U-ODIC100 U-DIC100 U-ODIC100
100XOI2
LUCPlanFLN 20X U-DIC20 U-ODIC20 U-DIC20 U-ODIC20
40X U-DIC40 U-ODIC40 U-DIC40 U-ODIC40
60X U-DIC60 U-ODIC60 U-DIC60 U-ODIC60
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DIC Slider
Top Lens U-TLD/IX2-TLW U-TLD/IX2-TLW U-TLO
UPlanFLN 10X2 U-DIC10HC U-DIC10HR ---
20X U-DIC20HC U-DIC20HR ---
40X U-DIC40HC U-DIC40HR ---
40XO U-DIC40HC U-DIC40HR ---
100XO2 U-DIC100HC U-DIC100HR U-ODIC100HR
100XOI2
U-DICTHC
(High Contrast type) U-DICTHR (High Resolution type)
UPlanSApo
PlanApoN
LUCPlanFLN
60X U-DIC60HC U-DIC60HR U-ODIC60HR
60XOI
10X U-DIC10HC U-DIC10HR ---
20X U-DIC20HC U-DIC20HR ---
20XO
40X2 U-DIC40HC U-DIC40HR ---
60XO U-DIC60HC U-DIC60HR U-ODIC60HR
60XW U-DIC60HC U-DIC60HR U-ODIC60HR
100XO U-DIC100HC U-DIC100HR U-ODIC100HR
60XO U-DIC60HC U-DIC60HR U-ODIC60HR
20X U-DIC20HC U-DIC20HR ---
40X U-DIC40HC U-DIC40HR ---
60X U-DIC60HC U-DIC60HR U-ODIC60HR
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Combinations of Objectives, DIC Sliders, Top Lenses and DIC Prisms
To be used in the BFP1 position of the DIC slider.
UIS Series
DIC Slider U-DICT U-DICTS (Shift type)
Top Lens U-TLD/IX2-TLW U-TLO U-TLD/IX2-TLW U-TLO
UPlanFl 10X U-DP10 --- U-DP10 ---
20X U-DP20 U-ODP20 U-DP20 U-ODP20
40X U-DP40 U-ODP40 U-DP40 U-ODP40
60XOI3*U-DPO60S U-ODPO60S U-DPO60S U-ODPO60S
100XO3*U-DP100 U-ODP100 U-DP100 U-ODP100
100XOI3*
UPlanApo 10X U-DP10 --- U-DP10 ---
10XO
20X U-DPA20 U-ODPA20 U-DPA20 U-ODPA20
20XO3*
40X U-DPA40 --- U-DPA40 ---
40XOI3*--- --- U-ODPO40S U-ODPO40S
60X U-DPA60 --- U-DPA60 ---
100XOI3*U-DP100 U-ODP100 U-DP100 U-ODP100
PlanApo 60XO3*--- --- U-DPO60S U-ODPO60S
*Usable regardless of the model number (3, 2 or none)
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DIC Slider U-DICT U-DICTS (Shift type)
Top Lens U-TLD/IX2-TLW U-TLO U-TLD/IX2-TLW U-TLO
UPlanApo 10XW3*U-DP10 --- U-DP10 ---
60XW3*U-DPO60S U-ODPO60S U-DPO60S U-ODPO60S
60XW3/IR*
UApo 20X3/340*--- --- --- ---
20XW3/340*
40X3/340*U-DPA40 --- U-DPA40 ---
40XW3/340*--- --- U-DPO40S U-ODPO40S
40XOI3/340*
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DIC Slider
Top Lens U-TLD/IX2-TLW U-TLD/IX2-TLW U-TLO
UPlanFl 10X --- U-DP10HR ---
20X U-DP20HC U-DP20HR ---
40X U-DP40HC U-DP40HR ---
60XOI3*U-DPO60HC U-DPO60HR U-ODPO60HR
100XO3*U-DP100HC U-DP100HR U-ODP100HR
100XOI3*
U-DICTHC
(High Contrast type) U-DICTHR (High Resolution type)
UPlanApo 10X --- U-DP10HR ---
10XO
20X --- U-DPA20HR ---
20XO3*
40X --- U-DPA40HR ---
40XOI3*--- --- ---
60X --- --- ---
100XOI3*U-DP100HC U-DP100HR U-ODP100HR
60XO3*U-DPO60HC U-DPO60HR U-ODPO60HR
10XW3*--- U-DP10HR ---
PlanApo
UPlanApo
60XW3*U-DPO60HC U-DPO60HR U-ODPO60HR
60XW3/IR*
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DIC Slider
Top Lens U-TLD/IX2-TLW U-TLD/IX2-TLW U-TLO
UApo 20X3/340*--- --- ---
20XW3/340*
U-DICTHC
(High Contrast type) U-DICTHR (High Resolution type)
40XW3/340*--- --- ---
20XOI3/340*
40X3/340*--- U-DPA40HR ---
2-3 Attaching the DIC Condenser
}Attach the DIC condenser by referring to the instruction manual for the IX2 series (IX81, IX71, IX51) or IX series (IX70, IX50)
microscope frame.
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2-4 Centering the DIC Condenser
Fig. 4
}Disengage the analyzer, polarizer, DIC slider and DIC prism from the light
path to obtain the brightfield observation light path.
1. Turn the condenser height fine adjustment knob @ counterclockwise to
loosen it, then push it fully to the right to raise the condenser to the higher
limit of fine adjustment.
2. Turn the condenser height adjustment knob ² in the direction of the ar-
row to lower the condenser to the lowest limit.
3. Set the aperture iris diaphragm lever ³ to open ( –
>\).
4. Set the field iris diaphragm lever | to open ( –
>\).
5. Use a 10X objective and bring the specimen into focus.
6. Stop down the field iris diaphragm lever until the field iris diaphragm
image comes inside the field of view.
7. Gently push the condenser height fine adjustment knob @ to the left to
bring the field iris diaphragm image into focus. When it is focused, turn the
knob clockwise and fix it there.
8. While gradually opening the field iris diaphragm, turn the condenser-
centering knob ƒ on the condenser holder so that the field iris diaphragm
image comes at the center of the field of view of the eyepiece.
9. Gradually open the field iris diaphragm until its image inscribes the field of
view. Now the DIC condenser is centered.
}In actual observation, open the field iris diaphragm until its image circum-
scribes the field of view.
Field iris diaphragm image
³
@
²
|
ƒ
Field of view of eyepiece
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OBSERVATION METHODS
3-1 Brightfield Observation
}Objectives with 10X or higher power can be used (20X or higher power is required when the U-TLO top lens is used).
With the U-TLD or IX2-TLW is used in combination with the 100X objective, the NA may be slightly insufficient, but this is not
serious enough to cause trouble in ordinary observations.
1. Disengage the analyzer, polarizer, DIC slider and DIC prism from the light path.
2. Engage a 10X objective in the light path and bring the specimen into focus.
3. Increase the objective power step by step and bring the specimen into focus every time; repeat this until the target
magnification is reached.
4. Adjust the field iris diaphragm so that it circumscribes the field of view.
5. Adjust the aperture iris diaphragm properly.
3-2 DIC Observation
}If a plastic petri dish is used, the DIC effect cannot be obtained due to the polarization characteristic of the plastic material.
Be sure to use a glass petri dish.
}The DIC observation requires the DIC prism and analyzer in addition to the IX2-DICD DIC condenser.
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Fig. 5
Fig. 6
1Cross-Nicol Adjustment (Figs. 5 and 7)
1. Engage the IX2-AN analyzer and DIC slider in the light path. (Refer to the
instruction manual for the IX2 series or IX series microscope frame.)
2. Remove the DIC prism slider @.
3. Push in the polarizer engagement knob ² to engage the polarizer in the
light path.
4. Engage the 10X objective in the light path, place an optimum specimen
for brightfield observation on the stage, bring the specimen into approxi-
mate focus and remove the specimen out of the light path.
5. When the U-BI90CT binocular observation tube is used, rotate the CT
turret ³ to position “CT” to engage the CT lens in the light path. (Fig. 6)
When the U-BI90 or U-TBI90 binocular observation tube or U-TR30H
trinocular observation tube is used, remove an eyepiece and attach the
U-CT30 centering telescope.
6. Rotate the focusing ring | (or, with the centering telescope, rotate the
knurled section) to bring the objective’s exit pupil into focus. (Fig. 6)
ƒ
@
²
…
³
|
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Fig. 7
7. Rotate the prism movement knob of the DIC slider in the clockwise direction
all the way until the knob is stopped. A black interference stripe then a rain-
bow-colored interference stripe will be observed. Here, stop the knob at the
position with which the black interference stripe can be seen. (Fig. 7)
8. While observing the objective’s exit pupil, rotate the polarizer rotation dial
ƒ on the polarizer unit until the black interference stripe becomes darkest.
This is the position for using the polarizer. (Figs. 5 & 7)
}If two black interference stripes are observed, rotate the polarizer by about
90° so that only one black interference stripe is visible.
}The “cross-Nicol” position is located near the 0° position indication.
9. After determining the position, tighten the polarizer-clamping knob … so
that the polarizer will not rotate.
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2Observation Procedure
1. Mount the DIC prism matching the objective to be used in the DIC prism slider, then insert this into the light path.
2. Engage the objective to be used in the light path.
}Only when an objective for which the use of the BFP1 position is specified (see pages 7 to 11) is used, push in the prism
position selector lever of the DIC slider to the BFP1 position.
3. Place the specimen on the stage and bring the specimen into focus by moving the objective up or down.
4. Adjust the field iris diaphragm so that its image circumscribes the field of view.
5. Stop down the aperture iris diaphragm to enhance the contrast.
6. Rotate the prism movement knob of the DIC slider to select the interference color that can provide the optimum contrast
in accordance with the specimen.
The background interference color is continuously variable from the gray sensitive color to purple
sensitive color.
The background interference color is continuously variable from black to light gray.
U-DICT:
U-DICTS:
U-DICTHC:
U-DICTHR:
· Setting the background color to dark enables an observation like darkfield observation.
· Setting the background color to gray provides observation with high contrast and 3D feeling with the gray sensitive color
with which the sensitivity is highest.
· Setting the background color to gray allows very small change in phase to be observed as a change in color. (U-DICT only)
· There is a directional characteristic with the detection sensitivity because of the configuration of the DIC prism. As a result,
the contrast may sometimes be improved by rotating the specimen on the stage.
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