Orflo moxi z User manual

1) Turn Moxi ON
Mini Automated
Cell Counter
Your Moxi Z comes with a partial battery charge
and can be used immediately. Alternatively, you
can charge the Moxi by connecting it to a
computer or power adapter via the supplied USB
cable. Be sure to disconnect the USB cable prior
to running samples.
Before you Begin:
- Moxi Z cassettes and diluent.
- Biological cell sample.
- Pipette and tips for 75μL aliquot.
Required Materials:
Quick Start Guide
-Type S Cassettes. Size range: 3 - 20um avg. dia.
Concentration range: 3,000 to 2,500,000 cells/ml
-Type M Cassettes. Size range: 4 - 25um avg. dia.
Concentration range: 3,000 - 500,000 cells/ml
For Adherent Cells: Aspirate media and detach
cells with appropriate disassociation media
(e.g., Accutase).
Sample Prep Options:
2) Depress Tray and
Insert Cassette
3) Load
75μL Sample
5) Data Summary
Nov 2011 Rev 3.7
Made in the USA
An electronic copy of the complete Moxi Z user
manual is on the USB ash drive that
was included in the Moxi Z kit.
User Manual:
Done 2-26umGating Delete
Dec 16 14:03:00
Count
0
500
1 305 10 15 20 25
Diameter (um)
TEST: 01
Curve Fit Count Average Size
1.28e+05 Cells/ml 9.218 um (dia)
1.55 pL (vol)
MPI= .99Type M
MPI - Moxi Population
Index
Curve Fit
Avg. Cell Size (dia. & vol.)
Count (within
curve t or gated
area)
Cell Size Scale Population Histogram
Cassette Type
Press for
Gating Mode
Touch
Gating
Dec 16 14:03:00
Count
0
500
1 305 10 15 20 25
Diameter (um)
Gated Count
1.28e+05/mL
DeleteBack
Type M MPI= .99
1.28e+05 Cells/ml 9.218 um (dia)
1.55 pL (vol)
Average Size
TEST: 01
2-26um
Curve Fit
Touch
Curve Fit
Curve Fit Results Gated Results
An electronic copy of MoxiChart is on the
USB ash drive that was included in the Moxi Z kit.
MoxiChart facilitates histogram downloading and
rmware updates via Bluetooth and is compatible
with PC and MAC.
MoxiChart Software:
Touch to Move Gates
System is Ready ...
Dec 16 14:03:00
Touch Screen When Loaded...
Back
Touch Here
Small Particle Mode
for
4) Touch Screen to Start
Touch Here
for Cells
< 8μm Dia.
Press to
Change Scale:
2-34, 2-26,
2-18, 2-10μm
Adjust Count Scale

To Obtain BlueTooth ID:
Made in the USA
Nov 2011 Rev 3.7
BlueTooth ID: xxxx:xxxx:xxxx
Model: BlueMod+P25/G2
FCC ID: T7V-BC06
IC: 216Q-BC06
See Moxi Z user manual for
PC/MAC setup information.
Dec 16 14:03:00
Back
Insert USB Flash Drive
into USB slot on the
MAC.
To Install MoxiChart Software on the MAC: To Install MoxiChart Software on the PC:
Insert USB Flash Drive
into USB slot on the
PC.
1) If prompted to run MoxiChart Install,
click YES. Follow on screen instructions.
OR
2) Open ORFLO DISK folder
(on Flash Drive) and run
MoxiChart Install. Follow
on screen instructions.
Quick Start Guide Mini Automated
Cell Counter
1) Open ORFLO DISK (on Flash Drive)
2) Double Click MoxiChartInstallMac.dmg
3) Drag MoxiChart Icon to Applications folder
4) Run MoxiChart by double clicking MoxiChart
Icon.
Touch
Bluetooth
on
Home Screen
Touch
Back
Saved Histograms:
Back Page DownPage Up Delete ALL
Dec 16 14:03:00
Count
0
500
1 305 10 15 20 25
Diameter(um)
Select a Histogram...
Count
0
500
1 305 10 15 20 25
Diameter(um)
Count
0
500
1 305 10 15 20 25
Diameter(um)
Count
0
500
1 305 10 15 20 25
Diameter(um)
Count
0
500
1 305 10 15 20 25
Diameter(um)
Count
0
500
1 305 10 15 20 25
Diameter(um)
Count
0
500
1 305 10 15 20 25
Diameter(um)
Count
0
500
1 305 10 15 20 25
Diameter(um)
Count
0
500
1 305 10 15 20 25
Diameter(um)
Select
Touch
Histogram
on
Home Screen
Test 8
May 23 18:03
Test 7
May 23 18:03
Test 6
May 23 18:03
Test 5
May 23 18:03
Test 4
May 23 18:03
Test 3
May 23 18:03
Test 2
May 23 18:03
Test 1
May 23 18:03
Test 4
May 23 18:03
Test
Delete
Dec 16 14:03:00
Count
0
500
1 305 10 15 20 25
Diameter (um)
TEST: 04
12.8/uL
Curve Fit Count Ave. Size
12.8 Cells/ul 9.2 um (diameter)
1.55 pL (volume)
MVI=99%
Back
BlueTooth ID.
When the unit is on,
it is always in Bluetooth
Pairing mode.
To Return to Home Screen
Gating
Adjust
Count
Scale