Oxford Optronix HypoxyLab Gen 1 User manual

HypoxyLab User Manual
Revision 1.6a | February 2022
1
User Manual
Bench-top hypoxia wor station
(Gen 1)

HypoxyLab User Manual
Revision 1.6a | February 2022
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Product Documentation
and User Manual
Revision 1.6a
Applicable to HypoxyLab ‘Generation 1’ systems bearing
serial numbers HL9004 – HL9067.
NOTE: certain user interface functionality described herein assumes
firmware version 1.814 or later is installed.
NOTE: certain hardware features described herein may not be applicable to
units bearing serial numbers prior to HL9030.
Designed and manufactured by:
Oxford Optronix Ltd.
19 & 21 East Central
127 Olympic Avenue
Milton Par
Oxford OX14 4SA
United Kingdom
t: [phone]
f: [phone]
Primary website: www.oxford-optronix.com
Sales email: [email protected]
Support website:
https://www.oxford-optronix.com/support/hypoxylab-support
Support email:
[email protected]
HypoxyLab™ is a registered trademar of Oxford Optronix Ltd.
© Oxford Optronix Ltd. 2022. All rights reserved.

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Table of Contents
1. S FETY I NFORM TION 5
1.1 Symbols used on HypoxyLab ......................................................... 5
1.2 Summary of Warnings .................................................................... 6
1.3 Summary of Cautions ..................................................................... 7
2. I NTRODUCTION 10
2.1 Optimising Conditions for Cell-Based Research .......................... 10
2.2 User Benefits of the HypoxyLab ................................................... 11
3. I NST LL TION R EQUIREMENTS 14
3.1 Gas Supply Requirements ........................................................... 14
3.2 Electrical Supply Requirements ................................................... 16
3.3 Physical and Location Requirements ........................................... 16
4. I NST LLING ND U SING H YPOXY L B 17
4.1 Support Videos ............................................................................. 17
4.2 List of Standard Components ....................................................... 17
4.3 Unpac ing and Description .......................................................... 17
4.4 Connecting the Electrical Supply .................................................. 19
4.5 Connecting the Gas Supplies ....................................................... 20
4.6 Water Supply / Waste Water ........................................................ 21
4.7 Fitting the Shelf Assemblies ......................................................... 22
4.8 Using the Armport Sleeves........................................................... 23
4.9 Fitting the Armport Plugs .............................................................. 24
4.10 Using the Easy-Entry Hatch ......................................................... 24
4.11 Fitting the Thermal Jac et ............................................................ 25
4.12 Fitting Insulated Armport Covers .................................................. 25
4.13 Removing the Enclosure Cover .................................................... 26
4.14 Changing the Armport Sleeves .................................................... 27
4.15 The Touchscreen User Interface .................................................. 27
4.16 Calibrating the CO
2
Sensor .......................................................... 28
4.17 Calibrating the oxygen sensor ...................................................... 29
4.18 Manual fan control (reducing evaporation) ................................... 30
4.19 Touchscreen disable function....................................................... 30
4.20 Saving Recorded Data to USB Flash Memory ............................. 31
4.21 Applying a Software Upgrade....................................................... 32
4.22 Using OxyLite™ for in situ Oxygen Measurements ...................... 32
4.23 Setting up the optional CytoSMART™ add-on ............................. 33
5. C RE ND M INTEN CE 34
5.1 Cleaning and Sanitising ............................................................... 34
5.2 Routine Preventative Maintenance .............................................. 34

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5.3 Returning HypoxyLab for Repair, Service, or Upgrade ................ 35
5.4 Storage Recommendations for HypoxyLab .................................. 35
6. O BT INING T ECHNIC L SSIST NCE 37
6.1 Technical Support ........................................................................ 37
6.2 Support Site and Support Videos ................................................. 37
7. T ECHNIC L S PECIFIC TIONS 38
8. W RR NTY 40
PPENDIX 1 - Converting between % oxygen and mmHg 39

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1. SFETY INFORM TION
This section contains important safety information related to the general use
of the HypoxyLab. Other important safety information appears throughout
this manual in the form of warnings and cautions.
1.1 Symbols used on HypoxyLab
SN
Serial number
REF
Catalogue number
(product code)
Date of manufacture
Equipment should
NOT be disposed of
in the normal waste
st
r
eam
Warning - see user
manual
Read user manual
N2
Nitrogen gas inlet
CO
2
Carbon dioxide gas
inlet
Air
Air gas inlet

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1.2 Summary of Warnings
A Warning indicates a condition leading to the possibility of serious injury or
even death to the operator/user.
W RNING
Li e any incubator, ALWAYS use HypoxyLab in a well-
ventilated room or laboratory.
W RNING
As for all tissue culture rooms or laboratories containing
incubators, we strongly recommend the use of wall/ceiling
mounted oxygen depletion warning sensors.
W RNING
While due care is ta en to ensure gas hose connectors and
regulator fittings supplied by Oxford Optronix are lea -free it
is ESSENTIAL to chec for gas lea s, both at the gas
source end and at the HypoxyLab end of gas lines once
they are connected and pressurized.
W RNING
DO NOT operate the HypoxyLab with the enclosure cover
removed. To do so may cause uncontrolled CO2 venting.
W RNING
DO NOT attempt to open the base of the HypoxyLab or the
turret structure within the chamber. There is a ris of
electrical shoc .
W RNING
DO NOT attempt to supply gases to the HypoxyLab other
than the ones specified.
W RNING
DO NOT exceed the maximum pressure ratings of the inlet
gases supplying the HypoxyLab.
W RNING
DO NOT exceed the maximum voltage ratings of the inlet
power supplying the HypoxyLab.
W RNING
ALWAYS replace fuses with the same type and rating.
Failure to do so may present a fire hazard.
W RNING
The rear surface of the turret structure (see Fig 4.1) may
become HOT TO THE TOUCH. Allow at least 5 minutes
from powering down the HypoxyLab before touching the
rear of the turret (e.g. to change the HEPA filter).

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W RNING
Failure to pac the HypoxyLab adequately for repair or
service may cause costly damage during transit.
W RNING
No user-serviceable parts inside. HypoxyLab MUST be
repaired or serviced by Oxford Optronix Ltd personnel only.
1.3 Summary of Cautions
A Caution indicates a condition that may lead to a malfunction and/or
permanent damage of the HypoxyLab.
C UTION
BEFORE UNPACKING please carefully inspect the outer
shipping carton for any signs of damage including tears,
dents and water stains. Please ta e photographs as evidence
and IMMEDIATELY report any pac aging damage to Oxford
Optronix Ltd or your authorised distributor.
C UTION
HEAVY ITEM. The fully pac aged HypoxyLab weighs
approximately 30 Kg (70 lbs). TWO PERSONS are required
to safely lift and move the HypoxyLab.
C UTION
FRAGILE TURRET. Never handle the turret structure to lift or
move the HypoxyLab. Move the HypoxyLab by gripping the
underside of the base only.
C UTION
Gas inlet lines and their integrity are the responsibility of the
user and should be constructed, tested and maintained in
accordance with standards specified by the Code of Practice
CP4 published by the British Compressed Gasses
Association (or international equivalent organisations).
C UTION
It is the responsibility of the user to fit multi-stage regulators
to all gas cylinders supplying gasses to the HypoxyLab.
C UTION
It is the responsibility of the user to fit the correct multi-stage
regulator according to the type of gas used.
C UTION
It is the responsibility of the user to ensure that supplied
gases are free of particulate contamination. Oxford Optronix
shall not be held responsible for damage caused by
particulates entering the HypoxyLab gas flow controllers.

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C UTION
DO NOT site the HypoxyLab near draughts caused by doors,
windows or air conditioning/ventilation ducts.
C UTION
DO NOT site the HypoxyLab on paper towels, tablecloths or
any other materials that might obstruct the air vents on the
underside of the HypoxyLab.
C UTION
DO NOT operate the HypoxyLab outside the specified
operating temperature range of 15 – 30
º
C.
C UTION
ONLY use DIONIZED / DISTILLED WATER to supply the
HypoxyLab. Oxford Optronix shall not be held responsible for
damage caused by the presence of scale in the humidifier
reservoir.
C UTION
It is ESSENTIAL to empty the humidifier reservoir during
periods when HypoxyLab is not in use. Oxford Optronix will
not be held responsible for damage caused by the build-up of
microbial contamination in the humidifier reservoir due to
stagnant water.
C UTION
Regular (monthly) decontamination of the internal water
reservoir is ESSENTIAL to prevent the build-up of microbial
contamination over time. Refer to Section 5.2.
C UTION
The armport sleeves should be maintained WITHIN the
armport covers during extended incubation periods (e.g.
overnight). Allowing the armport sleeves to ‘dangle’ outside
the HypoxyLab may result in the accumulation of significant
water condensation.
C UTION
DO NOT use sharp objects to operate the touch screen
display.
C UTION
DO NOT spray, pour or spill any liquid on the touch screen
display.
C UTION
DO NOT attempt to operate the HypoxyLab in the vicinity of
equipment that emits ionising radiation or produces a strong
magnetic field as the performance of the device may be
adversely affected.
C UTION
Use ONLY cables and accessories supplied by Oxford
Optronix Ltd for use with HypoxyLab.

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C UTION
Use ONLY the HEPA filter supplied by Oxford Optronix Ltd.

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2. INTRODUCTION
2.1 Optimising Conditions for Cell-Based Research
HypoxyLab™ offers researchers a new tool it to accurately reproduce real-
life physiological conditions for a wide variety of cell-based research fields,
including cancer biology, radiation cell biology, cardiovascular research,
apoptosis, neurology, stem cell research, multidisciplinary drug
development, and proteomics.
Interest in the role of hypoxia in cell development has gained momentum as
researchers learn more about the biology of physiological oxygen
concentration and how it affects cells at the molecular level. While in the
past the focus in the laboratory was on maintaining cells alive and
genetically stable while avoiding contamination, in recent decades
researchers have demonstrated that cells react in different ways, both
metabolically and morphologically, depending on the environmental factors
that maintain and interact with them.
Today’s life scientists require the ability to reproduce in vivo environments
in which cells can be cultured in their natural state, at oxygen
concentrations in the range of 0.5 - 10%. To date all hypoxia wor stations
present the user with a method for controlling oxygen as a percentage
concentration (% O
2
) only. However, this approach does not ta e account of
barometric pressure and therefore fails to deliver compensation for altitude
of the research location or prevailing climatic conditions.
HypoxyLab is a second-generation hypoxia wor station that uses the
absolute partial pressure of oxygen, expressed in units of mmHg or Pa, to
deliver authentic hypoxia replication with the highest possible accuracy.
Since the partial pressure of oxygen is what cells actually “see” when
exposed to oxygen, this scientifically superior approach enhances hypoxia
accuracy, particularly when culturing cells at extremely low oxygen set-
points. Regardless therefore, of whether a research laboratory is located in
Amsterdam or in Denver, hypoxia conditions generated within the
HypoxyLab in one location can be reliably compared with those generated
in another.
HypoxyLab delivers precise control of the partial pressure of oxygen and
carbon dioxide content, alongside chamber temperature and humidity, using
optimised PID algorithms alongside advanced digital gas flow controllers,
low maintenance or maintenance-free sensors, and built-in nebulizer-based
humidifier technology. Using its touchscreen display, researchers also have
the ability to set HypoxyLab to automatically cycle through up to eight fully
programmable oxygen profiles.

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HypoxyLab is also engineered to support our “gold standard” OxyLite™
dissolved oxygen monitors. Positioning an OxyLite sensor within a cell
culture provides a direct measure of the partial pressure of oxygen that cells
are actually exposed to and can optionally be recorded alongside the values
of chamber pO
2
, CO
2
, temperature, and humidity.
For research teams underta ing studies at the cellular and molecular level,
HypoxyLab provides a way to maintain cells at true physiological oxygen,
an essential prerequisite to refining the processes they are using to probe
gene expression changes, signal transduction pathways, enzyme activities,
and the molecular mechanisms that underlie these phenomena.
2.2 User Benefits of the HypoxyLab
True hypoxia replication
As every scientist nows, it’s the partial pressure of oxygen that reflects the
oxygen that cells actually ‘see’. The partial pressure of oxygen varies not
only with oxygen concentration in the atmosphere, but also with altitude and
prevailing weather conditions. We believe it is implausible to express
hypoxia in terms of a (barometrically uncompensated) percent oxygen
concentration value alone. HypoxyLab is the world’s first and only hypoxia
chamber that directly regulates its environment using the partial pressure of
oxygen, expressed in units of mmHg or Pa, and is thereby insensitive to
changing climatic and altitude conditions. This scientifically rigorous
approach enhances HypoxyLab's accuracy over other devices that rely
simply on % oxygen measurements.
n situ oxygen measurements from cell culture media
For those that require in situ oxygen measurements from the precise
location or layer where cells are actually growing (or simply from a bottle of
media), HypoxyLab provides a dedicated, sealable side-gland via which to
insert an OxyLite™ fibre-optic oxygen sensor. The OxyLite sensor is a
miniature, highly accurate, non-consuming optical based oxygen sensor
that can be easily positioned precisely within the cell culture to obtain
continuous, real-time dissolved oxygen measurements at the sensor tip. By
ta ing into account the oxygen gradient generated between the chamber
environment and cellular oxygen consumption processes occurring within
the media, the OxyLite sensor delivers the ‘holy grail’ for hypoxia
researchers - the ability to measure oxygen availability in the
microenvironment where cells are actually growing.
Contamination controlled environment
HypoxyLab includes a built-in (and replaceable) HEPA filtration system that
continuously scrubs the chamber atmosphere. This is capable of achieving
an atmosphere equivalent to, or better than ISO 14644-1 Class 2 within
30 seconds of power-up, ensuring that cultures and media are protected

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from the ris of contamination. Internal humidification within the chamber is
delivered using an ultrasound nebulizer rather than just a pan of water,
reducing another potential source of contamination.
Live cell imaging
We have teamed with the manufacturers of the CytoSMART™ system, an
extremely compact digital microscope solution that can be deployed within
the HypoxyLab and is built to withstand long-term exposure to tissue culture
environments. The system is supplied with an accompanying tablet PC,
which is ept outside the HypoxyLab and on which live cell culture images
are displayed. The CytoSMART™ system supports time-lapse recording
and cloud-based access to images and time-lapse data from any browser-
capable computer, smart phone or tablet device.
Unrivalled performance
Partial pressure of oxygen (pO2) as well as chamber CO2, temperature and
humidity are controlled via HypoxyLab’s touch-screen display and delivered
using digital gas flow controllers and state-of-the-art sensors. HypoxyLab is
the most faithfully accurate system available today.
Lightweight cover
HypoxyLab’s lightweight and durable cover means that it can be easily
removed by just one person. This not only allows for easy loading of media,
consumables but ma es routine cleaning/sterilisation of the wor station a
practical reality.
Oxygen profiling
The touch screen user interface provides an oxygen profiling function to
automatically cycle through up to eight oxygen levels. The fact that
HypoxyLab can adjust the hypoxia equilibrium in a matter of minutes (rather
than hours) adds real value to the concept of ‘oxygen profiling’ whilst
maintaining minimal levels of gas consumption.
Integrated touch-screen
HypoxyLab’s integrated full colour touch-screen conveniently allows the
user to control all aspects of the HypoxyLab whilst simultaneously
displaying real-time data in both digital and graphical format. The display
updates the user with vital information such as actual and set-point values
for gas composition, humidity and temperature and provides access to
useful features such as the oxygen profiling feature.
Easy-Entry system
HypoxyLab’s Easy-Entry hatch affords the quic and convenient transfer of
cells, culture-ware and media into the chamber. As soon as items are
passed through the hatch, HypoxyLab immediately applies the precise gas
composition necessary to maintain steady-state conditions. This immediate

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response effectively eliminates fluctuations in the pO2 of cell media during
operation of the Easy-Entry system. An internal ‘letterbox’ style flap acts as
an additional barrier against gas displacement when the hatch is opened.
Data logging
All HypoxyLab parameters are continuously recorded onto internal flash
memory and can be copied onto a USB flash drive via a port at the rear of
the HypoxyLab. Data files can be analyzed and played bac retrospectively
using the free LabChart Reader® by ADInstruments.
Ergonomic design
HypoxyLab’s patented ergonomic design ensures natural, relaxed
operation. The angled vision panel combined with touch-screen dimmable
LED illumination provide excellent visibility. Choose between bare-hand or
gloved operation using HypoxyLab’s easy-to-use cuff and sleeve system.
HypoxyLab also includes height-adjustable shelves and a 5V USB power
soc et within the chamber.
Cost of ownership advantage
HypoxyLab’s highly considered design minimises dead-space, ensures
rapid equilibration to set points and frugal gas consumption. Service-free
sensors and a user changeable HEPA filter simplify routine maintenance
and minimize HypoxyLab’s true cost of ownership.

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3. INST LL TION REQUIREMENTS
3.1 Gas Supply Requirements
W RNING
DO NOT attempt to supply gasses to the HypoxyLab other
than the ones specified. To do so may cause death or
injury.
W RNING
DO NOT exceed the maximum pressure ratings of the inlet
gasses supplying the HypoxyLab. To do so may cause
death or injury.
C UTION
The inlet gas lines which are attached to the HypoxyLab are
the responsibility of the user and should be constructed,
tested and maintained in accordance with standards
specified by the Code of Practice CP4 published by the
British Compressed Gasses Association (or international
equivalent organisations).
C UTION
It is the responsibility of the user to fit multi-stage regulators
to all gas cylinders supplying gasses to the HypoxyLab.
C UTION
It is the responsibility of the user to fit the correct multi-stage
regulator according to the type of gas used.
C UTION
Gases supplied to the HypoxyLab must be equal to or better
than 99.998% pure ‘zero’ grade (N4.8) nitrogen.
C UTION
It is the responsibility of the user to ensure that supplied
gases are free of particulate contamination. Oxford Optronix
shall not be held responsible for damage caused by
particulates entering the HypoxyLab gas flow controllers.
Only laboratory-grade (ideally 99.998% pure ‘zero’ grade (N4.8) purity)
gases must be used with the HypoxyLab. Where cylinders are used, it is
vital that high quality, multi-stage regulators are fitted to each gas cylinder
and that each regulator is correctly specified for the particular type of gas
used. Each regulator requires a 6 mm (¼ inch) NPT fitting to allow the
hoses supplied with the HypoxyLab to be connected (see Fig. 3.1 below).

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It is the responsibility of the user to ensure that supplied gases are
free of particulate contamination. Unfiltered gases entering the
HypoxyLab may damage the delicate electronic flow controllers within.
Oxford Optronix shall not be held responsible for damage caused by
particulates entering the HypoxyLab. We recommend the use of 30 µm
or finer upstream in-line filters for any building-wide ‘plumbed-in’
gases.
High quality, multi-stage regulators pre-fitted with NPT hose fittings can be
purchased from Oxford Optronix Ltd.
Figure 3.1 Example multi-stage regulator and barbed NPT fitting
The HypoxyLab requires CO
2
, synthetic air and nitrogen gases to be
supplied at pressures summarized below:
Gas type
Recommended
purity ‘N4.8’ or
better
Outlet Regulator Nominal outlet pressure
CO
2
6 mm (¼“)
NPT
Two Stage 1 – 4 bar (15 - 60 psi)
Synthetic air 6 mm (¼“)
NPT
Two Stage 1 – 4 bar (15 - 60 psi)
Nitrogen 6 mm (¼“)
NPT
Two Stage 3 – 5 bar (45 - 75 psi)
Gauges (internal bottle pressure +
controllable supply pressure)
Outlet connection. Requires 6 mm
(¼”) NPT ‘barbed’ hose-end fitting
(see inset below)
Connection to gas bottle (format and
thread standards are often country-
specific)
Supply pressure control tap
6 mm (¼”) NPT ‘barbed’
hose-end fitting

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3.2 Electrical Supply Requirements
W RNING
DO NOT exceed the maximum voltage ratings of the inlet
power supplying the HypoxyLab.
W RNING
ALWAYS replace fuses with the same type and rating.
Failure to do so may present a fire hazard.
The input voltage requirements for the HypoxyLab are as follows:
Input Voltage: 110 – 240 VAC 50/60 Hz Single phase
Max Operating Current: 5A
Max Operating Power: 550W
Fuses: 2 x 5A 20mm AS
A dedicated, country-specific IEC mains inlet cable is supplied with your
HypoxyLab .
3.3 Physical and Location Requirements
C UTION
DO NOT site the HypoxyLab near draughts caused by doors,
windows or air conditioning/ventilation ducts.
C UTION
DO NOT site the HypoxyLab on paper towels or tablecloths
or any other materials that might obstruct the vents on the
underside of the HypoxyLab.
C UTION
DO NOT operate the HypoxyLab outside the operating
temperature range of 15 – 30
º
C
HypoxyLab must be operated in a clean laboratory environment free from
corrosive gases, flammable gases and dust.
Instructions for assembling the (optional) electrically height-adjustable
mobile wor table (product code ‘HL_WORKTABLE’) are provided
separately.
If HypoxyLab is to be bench mounted, the bench must be stable, flat, level
and of sufficient size and strength to fully support the HypoxyLab.

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4. INST LLING ND USING HYPOXYLB
4.1 Support Videos
A collection of video tutorials covering many of the sections below is
maintained on the HypoxyLab support website:
http://www.oxford-optronix.com/support/supp_hypoxylab.htm
4.2 List of Standard Components
• HypoxyLab hypoxia wor station, including integrated HEPA filter
• 1 shelf assembly (standard)
• 3 inlet hoses including Swageloc ® fittings (3 m)
• 1 neoprene thermal jac et/cover
• 1 branded USB memory flash drive
• 1 enclosure-to-base main seal (fitted)
• 1 pair of armport sleeves with wrist seals (fitted)
• 1 spare pair of wrist seals
• 1 pair of wrist seal plugs
• 1 pair of insulated arm port covers
• 2 water fill/drain pipes (2 m)
• 1 bottle of wrist seal talcum powder, starch free
• 1 country-specific IEC power cable
• 1 user manual (this document)
4.3 Unpacking and Description
C UTION
BEFORE UNPACKING. Please carefully inspect the outer
shipping carton for any signs of damage including tears,
dents and water stains. Please ta e photographs as
evidence and IMMEDIATELY report any pac aging damage
to Oxford Optronix Ltd or our authorised distributor.
C UTION
HEAVY ITEM. The HypoxyLab weighs approximately 30 Kg
(70 lbs). TWO PERSONS are required to safely lift and move
the HypoxyLab.

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C UTION
FRAGILE TURRET. Never handle the turret to lift or move
the HypoxyLab. Move the HypoxyLab by gripping the
underside of the base only.
Before unpac ing the HypoxyLab, ensure the physical, electrical and gas
supply installation requirements listed in the previous section have been
followed.
When unpac ing the HypoxyLab, inspect the pac aging thoroughly for any
signs of damage that may have occurred during transit. Report any damage
immediately to Oxford Optronix Ltd or our authorised distributor.
Figure 4.1 Front view of the ypoxyLab (details may vary)
Turret
(internal)
LED light
(internal)
Enclosure
cover
Latch
Bleed
valve
Easy-Entry
hatch
Touch-screen
display
Base
Armport
(incl. cover)

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Figure 4.2 Close-up view of ypoxyLab rear connector panel (details may
vary)
To unpac the HypoxyLab, remove the retaining straps that surround the
shipping carton and that run under the wooden pallet, remove all protective
cellophane wrapping, open the top of the carton and remove the foam insert
protecting the top of the HypoxyLab.
Remove any pac ing tape connecting the base carton to the upper carton
then carefully lift the upper carton ‘up and over’ to reveal the HypoxyLab
secured within its base foam insert.
The HypoxyLab weighs approximately 30 g (70 lbs). It should be lifted out
of the base foam insert by TWO PERSONS by locating the gripping points
in the foam insert either side of the HypoxyLab.
Carefully position the HypoxyLab on a wor table or previously prepared
laboratory bench.
4.4 Connecting the Electrical Supply
Locate the IEC mains power cable (supplied in the accessories box) and
plug it into the mains inlet connector on the HypoxyLab bac panel (Fig.
4.2). Switch on the HypoxyLab, when ready to do so, by operating the
roc er switch.
Gas hose
connectors
IEC mains inlet
(containing fuses)
and on/off switch
USB flash drive
connector
Serial connector
for OxyLite
Drain hose
connector
Inlet hose
connector
(strictly de-ionized
water)

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4.5 Connecting the Gas Supplies
W RNING
As for all tissue culture rooms or laboratories containing
incubators, we strongly recommend the use of wall/ceiling
mounted oxygen depletion warning sensors.
W RNING
While due care is ta en to ensure gas hose connectors and
regulator fittings supplied by Oxford Optronix are lea -free it
is ESSENTIAL to chec for gas lea s, both at the gas
source end and at the HypoxyLab end of gas lines once
they are connected and pressurized.
The incoming gas supplies are the sole responsibility of the user. It is
essential that the user has read and fully understood Section 3.1 ‘Gas
Supply Requirements’ before connecting the gas supply hoses.
Three ‘Quic Connect’ style gas connection hoses, pre-fitted with high
quality Swageloc ® fittings are supplied with your HypoxyLab. These hoses
are for supplying CO
2
, air and nitrogen and are labelled accordingly. The
connector assemblies are supplied ‘clean’. The hoses should be attached to
the outlet stage of each regulated cylinder according to the table below.
Ta e a moment to double-chec that each labelled hose is securely
connected to the correct gas (cylinder). Also, double-chec that the
regulator output pressures are correctly set according to the table below.
Locate and identify the individual male inlet gas connectors on the bac of
the HypoxyLab. Push the brass Swageloc ® fitting end of each labelled gas
hose firmly onto the corresponding gas inlet connector, gripping the fitting
by the body rather than by the front flare (as shown above). An audible
‘clic ’ confirms that the Swageloc ® fitting is secured and that gas will flow.