Cooper Surgical K-Systems L100 IVF Series User manual

K-SYSTEMS®
IVF L100 Series Workstations
Instruction Manual

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
TABLE OF CONTENTS
1 GENERAL INFORMATION & SERVICE, 6
1.1 General Description, 6
1.2 Operating Principles and Features, 6
1.3 Copyright, 6
1.4 Customer Service Contact Information, 6
2 GENERAL OPERATIONAL INSTRUCTIONS, 7
2.1 Pre-Operational Notes, 7
2.2 Moving the Unit, 7
2.3 Instruction of the Operating Personnel, 8
2.4 Applicability of the Instructions, 8
2.5 Warranty, 8
3 INTRODUCTION, 9
3.1 Safety Symbols Used on the Unit, 9
3.2 Precautions/Warnings, 10
3.3 Important Safety Instructions, 10
4 GENERAL DESCRIPTION, 11
4.1 Setup Precautions, 11
4.2 Electro Magnetic and Other Interferences, 11
4.3 Ambient Condition, 12
5 FEATURES, 12
5.1 Laminar Flow, 12
5.1.1 Flow Pattern Diagram, 12
5.1.2 Pre-lter, 13
5.1.3 Fan, 13
5.1.4 Main Filter, 13
5.1.5 Gassing Station, 14
5.1.6 Gas Mixture, 14
5.2 Stereo Microscope, 14
5.3 Interior Light, 15
5.4 Power Supply, 15
5.5 Electrical Outlet, 16
5.6 Front Window, 16
6 KEYBOARD FUNCTIONS, 17
6.1 The Interior Light, 18
6.1.1 Operating the Laminar Air Flow, 18
6.2 Heating System, 18
6.2.1 Table Heating Unit, 18
6.2.2 Setting the Temperature, 18
6.2.3 Alarm, 20

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
6.3 Alarm Key, 21
6.4 Keyboard Lock, 21
6.5 Keyboard Unlock, 21
6.6 Operating the Microscope Light, 21
6.7 Menu Function, 22
6.8 Overview of Menu Functions, 23
6.9 User Menu (uSEr), 24
6.9.1 Timer for the Fan (FAn), 24
6.9.2 Timer for the Heat (hEAt), 26
6.9.3 Auto-Start (A-St), 27
6.9.4 Data Logging (Serial Communication) of Temperature through RS232, 28
6.9.5. The Dual Set-Point (SP), 28
6.9.6. Temperature Unit (uNIT), 29
6.10 Time Menu (Ti), 30
6.10.1 Time Setting (ti.St), 31
6.10.2 Start Set (St.St), 32
6.10.3 Show of Time (hour), 33
6.10.4 UV Light On (u-on), 35
6.10.5 UV Light Off (u-of), 36
6.11 Setup Menu (StuP), 37
6.11.1 Fan Speed (Fn.SP), 38
6.11.2 Calibrate Value Zone-1 (tn-1), 39
6.11.3. Calibrate Value Zone-2 (tn-2), 40
6.11.4 Calibrate Value Zone-3 (tn-3), 41
6.11.5 Calibrate Value Zone-4/Zone-L (tn-4/tn-L), 42
6.11.6 Calibrate Value Zone-5 (tn-5), 43
6.11.7 Calibrate Value Zone-6 (tn-6), 44
6.11.8 Calibrate Value of Tunnel (tn-t), 45
6.11.9 Calibrate Value Heated Glass/Heated Glass on Right Side L126 Dual (hS-1), 46
6.11.10 Calibrate Value Heated Glass: Left Side (hS-2), 47
6.12 Info Menu (info), 48
6.12.1 Version Number (vEr), 49
6.12.2 Reset (rESt), 50
7 DIFFERENT HEATED SURFACES, 51
8 CONNECTIONS, 53
8.1 Connectors on the Back, 53
8.1.1 Circuit Fuses, 53
8.1.2 Mains Connection, 53
8.1.3 Gas Connections, 54
9 TEMPERATURE CALIBRATION, 55
9.1 Calibrating Temperature on the Tabletop, 56
9.2 Calibrating of Heated Glass, 58
10 FUSES, 59

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
11 GASSING STATION OPERATION, 60
11.1 Establish the Flow Rate, 60
11.2 Humidifying the Gas Mixture, 61
11.3 Purging, 61
11.4 Operating the Microscope Light, 62
12 LIGHT SOURCE (LS112/LS114 LED), 62
12.1 Unpacking and Inspection, 62
12.2 General Description, 62
12.3 Installation, 63
12.4 Operating Instructions, 63
12.4.1 “ON” Function, 63
12.4.2 To Increase Light Intensity, 63
12.4.3 To Decrease Light Intensity, 63
12.4.4 “OFF” Function, 64,
12.4.5 Adjusting the Mirror, 64
12.5 Maintenance, 65
12.5.1 Cleaning, 65
12.5.2 Replacement of LED Lamp House, 65
12.6 Accessories, 65
12.7 Troubleshooting, 65
12.8 Technical Data, 65
12.9 Spare Parts LS112/LS114 Light Source, 66
13 TROUBLESHOOTING, 67
14 MAINTENANCE, 69
14.1 Routine Maintenance, 69
14.1.1 Daily Maintenance, 69
14.1.2 Monthly Maintenance, 69
13.1.2.1 Rinsing of Humidication Procedure, 70
14.1.3 Three Month Maintenance, 71
14.1.4 Annual Maintenance, 71
14.2 Specic Service Operations, 71
14.2.1 Replacing the Pre-lter, 71
14.2.2 Replacing the Interior Light, 72
15 MONITOR, 73
15.1 Description, 73
15.2 Connectors and Cables, 73
15.2.1 Composite Video Connector, 73
15.2.2 Composite to S-VHS Adapter, 73
15.2.3 S-VHS Video andAudio Connector, 73
15.2.4 DE–15 Male VGA Connector, 74
15.3 Connecting the Monitor to a Computer, 74
15.4 Connecting to a Camera, 74
15.4.1 BNC Connectors, 74
15.4.2 BNC to Composite, 74
15.5 Service, 74
15.6 Monitor Controls and Functions, 75

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
16 DISPOSAL PROCEDURE, 81
17 TECHNICAL SPECIFICATIONS, 81
18 ACCESSORIES, 83
18.1 Warming Blocks, 83
18.2 Mixed Gas Supply, 83
18.3 Gas Washing Bottles, 83
19 WARRANTY INFORMATION AND LIMITS ON LIABILITY, 84
19.1 Returned Goods Policy, 85
20 CUSTOMER SERVICE CONTACT INFORMATION, 86
21 EXPLANATION OF SYMBOLS, 86

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
1 GENERAL INFORMATION & SERVICE
1.1 General Description
The IVF K-SYSTEMS®L100 Series is a complete workstation for human and animal reproduction. It has
a warmed stainless steel table plate and the table plate maintains a temperature in the range
35-43 °C. The L124/L126 is heated by means of an electronically controlled heating system.
The vertical laminar airow passes through a HEPA main lter and protects the cell culture against
contamination.
1.2 Operating Principles and Features
The workstation provides a conned workspace in which the stable vertical unidirectional ow (laminar
ow) protects the product that is being handled against particulate contamination from the surroundings
and the operator.
All operations take place through the front opening. Pressure in the work chamber keeps the clean air
owing from the work chamber to the surroundings, avoiding the introduction of particulate contamination
to the work chamber.
1.3 Copyright
This manual contains information that is subject to copyright. All rights reserved. This manual should
not be photocopied, otherwise copied or distributed, completely or in part, without the approval of
CooperSurgical, Inc.
Users of K-SYSTEMS products should not hesitate to contact us if there are any unclear points or
ambiguities in this manual.
1.4 Customer Service Contact Information
European Service Representatives U.S. Service Representatives
ORIGIO a/s
Knardrupvej 2
2760 Måløv
Denmark
Customer Service:
Tel: [phone]
Fax: [phone]
New Purchases:
[e-mail]
Service Related E-mail:
ser[email protected]
www.origio.com
CooperSurgical, Inc.
95 Corporate Drive
Trumbull, CT 06611 USA
Customer Service:
Phone: [phone]
Fax: [phone]
International
Phone: [phone]
Fax: [phone]
Service Related E-mail:
ser[email protected]s.com
www.coopersurgical.com

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
CAUTION
If the equipment is used in a manner not specied by this manual, the safety
of the user may be at risk and the equipment may be damaged. Always use the
equipment as outlined in this Instruction Manual.
2 GENERAL OPERATIONAL INSTRUCTIONS
2.1 Pre-Operational Notes
• The workstation fan must be run at normal speed for at least 15 minutes prior to working inside
the workstation.
   i A green control light indicates proper operation.
• The work chamber is to be carefully cleaned and/or disinfected (See Section 14: Maintenance).
• For reliable operation it is important that the air-ow conditions are as undisturbed as possible.
Therefore, never overload the work chamber - insert only those utensils necessary for the actual
work.
• Objects and utensils must be carefully cleaned and/or disinfected before bringing them into the
work chamber.
• Necessary utensils for use during work must be placed within reach to avoid unnecessary
movement inside the workstation.
• Put on necessary personal clothing for reducing particle emission from operator
(i.e. gloves, masks and general clean room clothing). Special attention should be
given to hands and lower parts of the arms, as these are the parts of the operator most likely to
emit particles near the product.
• All work in the workstation must be performed with tranquil movements. Rapid arm movements in
the chamber may cause slipstreams, which will draw contaminated air into the work chamber.
• The number of transports into the work chamber must be minimized.
• Transport of possibly contaminated material may create airows that can contaminate the
product.
2.2 Moving the Unit
The L100 is designed as a stationary unit and it should not be moved once it has been installed in the
proper way according to descriptions in the Installation Manual.
If the workstation must be moved, it is strongly recommended that lifting equipment always be used due
to the weight of the device.
Use the lifting gear to move the unit by lifting in all 4 corners of the table top upward at the same time. It
is recommended that 1 person controls the lifting gear and at least 4 more people support each side of
the unit while the unit is being moved around.
CAUTION
Never try to lift or move the unit alone.
Never try to move the unit by lifting the middle part of tabletop.
Never use the handles found on each side of the unit to lift the unit with.

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
Always make sure to wear shoes that protect your feet while moving around the unit. During
transportation on medium long and long distances it is strongly recommended to use the original casing
that was delivered with the unit.
2.3 Instruction of the Operating Personnel
These operating instructions describe the L100 Workstation series covering the following models:
• L124 IVF Workstation,
• L126 IVF Workstation,
• L126 DUAL IVF Workstation,
• L126 MP Workstation
• L124 ICSI Workstation.
The L100 IVF Workstations have been manufactured in keeping with the latest technologies and
developments. It has been tested during assembly and before delivery to ensure correct operation.
However, it may present potential hazards to user, if this unit is used in conditions that lies outside of its
intentional way of use.
It is strongly recommended that:
• Only trained and authorized personnel perform any operation on the cabinet.
• Only trained and authorized service personnel perform any repairs to the device.
CAUTION
If the equipment is used in a manner not specied by this manual, the safety
of the user may be at risk and the equipment may be damaged. Always use the
equipment as outlined in this instruction manual.
2.4 Applicability of the Instructions
• Keep these instructions close to the device. This way you ensure having easy access to the
safety instructions and important information.
• Please note that the contents of this manual are subject to change without further notice.
• If you encounter problems that are not mentioned in this manual in detailed, please contact your
local Customer Service Representative for more information.
2.5 Warranty
CooperSurgical, Inc. warrants the operational safety and correct system operation of the whole
unit under the condition that:
• The device is operated as described in the manuals
• The device has not been modied
• All service intervals are kept according to manuals
• Only original spare parts and accessories that have been approved by CooperSurgical are used

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
3 INTRODUCTION
3.1 Safety Symbols Used on the Unit
The L100 Workstation contains high voltage components that may be hazardous. It is strongly
recommended not to remove the back cover. This equipment doesn’t contain any user serviceable
parts inside. It is recommended to have qualied service personnel perform any service needed on the
equipment.
Fuse Label
High Voltage Hazard Label
Warning Label on Mains Wire (Schuko).
Product Model Label
Warranty Label – Warning
Ground Label
Earth Warning
UV Light Warning (Optional,
only if UV Light is Installed)
Warning for 110V Countries
Warning for 230/240V Countries

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
3.2 Precautions / Warnings
• Read and understand the user manual completely before use.
• Do not use product if package is damaged.
• Do not use device without the front windows correctly attached.
• Perform temperature calibration in the intervals described in the manuals.
• Do not use this product at temperatures exceeding 30 °C.
• Always use HEPA lter for input gas to unit.
• Never use a non K-SYSTEMS lter.
• Use only premix gas (eg.5% CO2in air).
• Make sure that the gas supply pressure is kept stable at 0.5-0.7 bar.
• The power cord is to be used for mains disconnection.
• Always connect power cord to a proper grounded outlet.
• To reduce the risk of re or electric shock, this equipment should not be exposed to rain
or moistures and objects lled with liquids.
• Always use fuses according to the manufacturer descriptions.
3.3 Important Safety Instructions
• Read this safety instruction carefully before using equipment.
• Always keep these instructions.
• Heed all warnings.
• Follow all instructions.
• Refer all servicing to qualied service personnel.
• Do not use this apparatus near water.
• Do not block any ventilation openings.
• Do not install near any heat sources such as radiators, heat registers, stoves or other apparatus
that produce heat.
• Do not defeat the safety purpose of the grounding-type plug. Agrounding type plug has two
blades and a third prong is provided for your safety. If the provided plug does not t into your
outlet, consult and electrician for replacement of the obsolete outlet.
• Protect the power cord from being walked on or pinched, particularly at plugs, convenience
receptacles, and the point where they exit from the unit.
• Only use attachments/accessories specied by the manufacturer.
• Use only with the cart and stand specied by the manufacturer or sold with the unit.
• Unplug the unit during lightning, storms or when unused for a long period of time.
• Servicing is required according to service manual or if the unit has been damaged in any way,
such as if it has been dropped, exposed to rain or moisture, or does not operate normally.