Cooper Surgical K-Systems L100 IVF Series User manual

K-SYSTEMS®
IVF L100 Series Workstations
Instruction Manual

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
TABLE OF CONTENTS
1 GENERAL INFORMATION & SERVICE, 6
1.1 General Description, 6
1.2 Operating Principles and Features, 6
1.3 Copyright, 6
1.4 Customer Service Contact Information, 6
2 GENERAL OPERATIONAL INSTRUCTIONS, 7
2.1 Pre-Operational Notes, 7
2.2 Moving the Unit, 7
2.3 Instruction of the Operating Personnel, 8
2.4 Applicability of the Instructions, 8
2.5 Warranty, 8
3 INTRODUCTION, 9
3.1 Safety Symbols Used on the Unit, 9
3.2 Precautions/Warnings, 10
3.3 Important Safety Instructions, 10
4 GENERAL DESCRIPTION, 11
4.1 Setup Precautions, 11
4.2 Electro Magnetic and Other Interferences, 11
4.3 Ambient Condition, 12
5 FEATURES, 12
5.1 Laminar Flow, 12
5.1.1 Flow Pattern Diagram, 12
5.1.2 Pre-lter, 13
5.1.3 Fan, 13
5.1.4 Main Filter, 13
5.1.5 Gassing Station, 14
5.1.6 Gas Mixture, 14
5.2 Stereo Microscope, 14
5.3 Interior Light, 15
5.4 Power Supply, 15
5.5 Electrical Outlet, 16
5.6 Front Window, 16
6 KEYBOARD FUNCTIONS, 17
6.1 The Interior Light, 18
6.1.1 Operating the Laminar Air Flow, 18
6.2 Heating System, 18
6.2.1 Table Heating Unit, 18
6.2.2 Setting the Temperature, 18
6.2.3 Alarm, 20

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
6.3 Alarm Key, 21
6.4 Keyboard Lock, 21
6.5 Keyboard Unlock, 21
6.6 Operating the Microscope Light, 21
6.7 Menu Function, 22
6.8 Overview of Menu Functions, 23
6.9 User Menu (uSEr), 24
6.9.1 Timer for the Fan (FAn), 24
6.9.2 Timer for the Heat (hEAt), 26
6.9.3 Auto-Start (A-St), 27
6.9.4 Data Logging (Serial Communication) of Temperature through RS232, 28
6.9.5. The Dual Set-Point (SP), 28
6.9.6. Temperature Unit (uNIT), 29
6.10 Time Menu (Ti), 30
6.10.1 Time Setting (ti.St), 31
6.10.2 Start Set (St.St), 32
6.10.3 Show of Time (hour), 33
6.10.4 UV Light On (u-on), 35
6.10.5 UV Light Off (u-of), 36
6.11 Setup Menu (StuP), 37
6.11.1 Fan Speed (Fn.SP), 38
6.11.2 Calibrate Value Zone-1 (tn-1), 39
6.11.3. Calibrate Value Zone-2 (tn-2), 40
6.11.4 Calibrate Value Zone-3 (tn-3), 41
6.11.5 Calibrate Value Zone-4/Zone-L (tn-4/tn-L), 42
6.11.6 Calibrate Value Zone-5 (tn-5), 43
6.11.7 Calibrate Value Zone-6 (tn-6), 44
6.11.8 Calibrate Value of Tunnel (tn-t), 45
6.11.9 Calibrate Value Heated Glass/Heated Glass on Right Side L126 Dual (hS-1), 46
6.11.10 Calibrate Value Heated Glass: Left Side (hS-2), 47
6.12 Info Menu (info), 48
6.12.1 Version Number (vEr), 49
6.12.2 Reset (rESt), 50
7 DIFFERENT HEATED SURFACES, 51
8 CONNECTIONS, 53
8.1 Connectors on the Back, 53
8.1.1 Circuit Fuses, 53
8.1.2 Mains Connection, 53
8.1.3 Gas Connections, 54
9 TEMPERATURE CALIBRATION, 55
9.1 Calibrating Temperature on the Tabletop, 56
9.2 Calibrating of Heated Glass, 58
10 FUSES, 59

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
11 GASSING STATION OPERATION, 60
11.1 Establish the Flow Rate, 60
11.2 Humidifying the Gas Mixture, 61
11.3 Purging, 61
11.4 Operating the Microscope Light, 62
12 LIGHT SOURCE (LS112/LS114 LED), 62
12.1 Unpacking and Inspection, 62
12.2 General Description, 62
12.3 Installation, 63
12.4 Operating Instructions, 63
12.4.1 “ON” Function, 63
12.4.2 To Increase Light Intensity, 63
12.4.3 To Decrease Light Intensity, 63
12.4.4 “OFF” Function, 64,
12.4.5 Adjusting the Mirror, 64
12.5 Maintenance, 65
12.5.1 Cleaning, 65
12.5.2 Replacement of LED Lamp House, 65
12.6 Accessories, 65
12.7 Troubleshooting, 65
12.8 Technical Data, 65
12.9 Spare Parts LS112/LS114 Light Source, 66
13 TROUBLESHOOTING, 67
14 MAINTENANCE, 69
14.1 Routine Maintenance, 69
14.1.1 Daily Maintenance, 69
14.1.2 Monthly Maintenance, 69
13.1.2.1 Rinsing of Humidication Procedure, 70
14.1.3 Three Month Maintenance, 71
14.1.4 Annual Maintenance, 71
14.2 Specic Service Operations, 71
14.2.1 Replacing the Pre-lter, 71
14.2.2 Replacing the Interior Light, 72
15 MONITOR, 73
15.1 Description, 73
15.2 Connectors and Cables, 73
15.2.1 Composite Video Connector, 73
15.2.2 Composite to S-VHS Adapter, 73
15.2.3 S-VHS Video andAudio Connector, 73
15.2.4 DE–15 Male VGA Connector, 74
15.3 Connecting the Monitor to a Computer, 74
15.4 Connecting to a Camera, 74
15.4.1 BNC Connectors, 74
15.4.2 BNC to Composite, 74
15.5 Service, 74
15.6 Monitor Controls and Functions, 75

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
16 DISPOSAL PROCEDURE, 81
17 TECHNICAL SPECIFICATIONS, 81
18 ACCESSORIES, 83
18.1 Warming Blocks, 83
18.2 Mixed Gas Supply, 83
18.3 Gas Washing Bottles, 83
19 WARRANTY INFORMATION AND LIMITS ON LIABILITY, 84
19.1 Returned Goods Policy, 85
20 CUSTOMER SERVICE CONTACT INFORMATION, 86
21 EXPLANATION OF SYMBOLS, 86

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
1 GENERAL INFORMATION & SERVICE
1.1 General Description
The IVF K-SYSTEMS®L100 Series is a complete workstation for human and animal reproduction. It has
a warmed stainless steel table plate and the table plate maintains a temperature in the range
35-43 °C. The L124/L126 is heated by means of an electronically controlled heating system.
The vertical laminar airow passes through a HEPA main lter and protects the cell culture against
contamination.
1.2 Operating Principles and Features
The workstation provides a conned workspace in which the stable vertical unidirectional ow (laminar
ow) protects the product that is being handled against particulate contamination from the surroundings
and the operator.
All operations take place through the front opening. Pressure in the work chamber keeps the clean air
owing from the work chamber to the surroundings, avoiding the introduction of particulate contamination
to the work chamber.
1.3 Copyright
This manual contains information that is subject to copyright. All rights reserved. This manual should
not be photocopied, otherwise copied or distributed, completely or in part, without the approval of
CooperSurgical, Inc.
Users of K-SYSTEMS products should not hesitate to contact us if there are any unclear points or
ambiguities in this manual.
1.4 Customer Service Contact Information
European Service Representatives U.S. Service Representatives
ORIGIO a/s
Knardrupvej 2
2760 Måløv
Denmark
Customer Service:
Tel: [phone]
Fax: [phone]
New Purchases:
[e-mail]
Service Related E-mail:
ser[email protected]
www.origio.com
CooperSurgical, Inc.
95 Corporate Drive
Trumbull, CT 06611 USA
Customer Service:
Phone: [phone]
Fax: [phone]
International
Phone: [phone]
Fax: [phone]
Service Related E-mail:
ser[email protected]s.com
www.coopersurgical.com

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
CAUTION
If the equipment is used in a manner not specied by this manual, the safety
of the user may be at risk and the equipment may be damaged. Always use the
equipment as outlined in this Instruction Manual.
2 GENERAL OPERATIONAL INSTRUCTIONS
2.1 Pre-Operational Notes
• The workstation fan must be run at normal speed for at least 15 minutes prior to working inside
the workstation.
   i A green control light indicates proper operation.
• The work chamber is to be carefully cleaned and/or disinfected (See Section 14: Maintenance).
• For reliable operation it is important that the air-ow conditions are as undisturbed as possible.
Therefore, never overload the work chamber - insert only those utensils necessary for the actual
work.
• Objects and utensils must be carefully cleaned and/or disinfected before bringing them into the
work chamber.
• Necessary utensils for use during work must be placed within reach to avoid unnecessary
movement inside the workstation.
• Put on necessary personal clothing for reducing particle emission from operator
(i.e. gloves, masks and general clean room clothing). Special attention should be
given to hands and lower parts of the arms, as these are the parts of the operator most likely to
emit particles near the product.
• All work in the workstation must be performed with tranquil movements. Rapid arm movements in
the chamber may cause slipstreams, which will draw contaminated air into the work chamber.
• The number of transports into the work chamber must be minimized.
• Transport of possibly contaminated material may create airows that can contaminate the
product.
2.2 Moving the Unit
The L100 is designed as a stationary unit and it should not be moved once it has been installed in the
proper way according to descriptions in the Installation Manual.
If the workstation must be moved, it is strongly recommended that lifting equipment always be used due
to the weight of the device.
Use the lifting gear to move the unit by lifting in all 4 corners of the table top upward at the same time. It
is recommended that 1 person controls the lifting gear and at least 4 more people support each side of
the unit while the unit is being moved around.
CAUTION
Never try to lift or move the unit alone.
Never try to move the unit by lifting the middle part of tabletop.
Never use the handles found on each side of the unit to lift the unit with.

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
Always make sure to wear shoes that protect your feet while moving around the unit. During
transportation on medium long and long distances it is strongly recommended to use the original casing
that was delivered with the unit.
2.3 Instruction of the Operating Personnel
These operating instructions describe the L100 Workstation series covering the following models:
• L124 IVF Workstation,
• L126 IVF Workstation,
• L126 DUAL IVF Workstation,
• L126 MP Workstation
• L124 ICSI Workstation.
The L100 IVF Workstations have been manufactured in keeping with the latest technologies and
developments. It has been tested during assembly and before delivery to ensure correct operation.
However, it may present potential hazards to user, if this unit is used in conditions that lies outside of its
intentional way of use.
It is strongly recommended that:
• Only trained and authorized personnel perform any operation on the cabinet.
• Only trained and authorized service personnel perform any repairs to the device.
CAUTION
If the equipment is used in a manner not specied by this manual, the safety
of the user may be at risk and the equipment may be damaged. Always use the
equipment as outlined in this instruction manual.
2.4 Applicability of the Instructions
• Keep these instructions close to the device. This way you ensure having easy access to the
safety instructions and important information.
• Please note that the contents of this manual are subject to change without further notice.
• If you encounter problems that are not mentioned in this manual in detailed, please contact your
local Customer Service Representative for more information.
2.5 Warranty
CooperSurgical, Inc. warrants the operational safety and correct system operation of the whole
unit under the condition that:
• The device is operated as described in the manuals
• The device has not been modied
• All service intervals are kept according to manuals
• Only original spare parts and accessories that have been approved by CooperSurgical are used

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
3 INTRODUCTION
3.1 Safety Symbols Used on the Unit
The L100 Workstation contains high voltage components that may be hazardous. It is strongly
recommended not to remove the back cover. This equipment doesn’t contain any user serviceable
parts inside. It is recommended to have qualied service personnel perform any service needed on the
equipment.
Fuse Label
High Voltage Hazard Label
Warning Label on Mains Wire (Schuko).
Product Model Label
Warranty Label – Warning
Ground Label
Earth Warning
UV Light Warning (Optional,
only if UV Light is Installed)
Warning for 110V Countries
Warning for 230/240V Countries

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
3.2 Precautions / Warnings
• Read and understand the user manual completely before use.
• Do not use product if package is damaged.
• Do not use device without the front windows correctly attached.
• Perform temperature calibration in the intervals described in the manuals.
• Do not use this product at temperatures exceeding 30 °C.
• Always use HEPA lter for input gas to unit.
• Never use a non K-SYSTEMS lter.
• Use only premix gas (eg.5% CO2in air).
• Make sure that the gas supply pressure is kept stable at 0.5-0.7 bar.
• The power cord is to be used for mains disconnection.
• Always connect power cord to a proper grounded outlet.
• To reduce the risk of re or electric shock, this equipment should not be exposed to rain
or moistures and objects lled with liquids.
• Always use fuses according to the manufacturer descriptions.
3.3 Important Safety Instructions
• Read this safety instruction carefully before using equipment.
• Always keep these instructions.
• Heed all warnings.
• Follow all instructions.
• Refer all servicing to qualied service personnel.
• Do not use this apparatus near water.
• Do not block any ventilation openings.
• Do not install near any heat sources such as radiators, heat registers, stoves or other apparatus
that produce heat.
• Do not defeat the safety purpose of the grounding-type plug. Agrounding type plug has two
blades and a third prong is provided for your safety. If the provided plug does not t into your
outlet, consult and electrician for replacement of the obsolete outlet.
• Protect the power cord from being walked on or pinched, particularly at plugs, convenience
receptacles, and the point where they exit from the unit.
• Only use attachments/accessories specied by the manufacturer.
• Use only with the cart and stand specied by the manufacturer or sold with the unit.
• Unplug the unit during lightning, storms or when unused for a long period of time.
• Servicing is required according to service manual or if the unit has been damaged in any way,
such as if it has been dropped, exposed to rain or moisture, or does not operate normally.

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
4 GENERAL DESCRIPTION
IMPORTANT: THIS UNIT DOES NOT CONTAIN ANY USER-SERVICEABLE PARTS. PLEASE
LEAVE ALL MAINTENANCE WORK TO QUALIFIED PERSONNEL.
4.1 Setup Precautions
To avoid any possible damage to this system, follow all setup precautions described
in this section.
Finding a Suitable Location
Place this unit on a at, hard and stable surface.
Never place other heat generating equipment under the tabletop.
Space for Ventilation
Place this unit in a location with adequate ventilation to prevent internal heat build up. Allow at least 2 cm
clearance from the rear, 30 cm from the top and 20 cm from left and right to prevent overheating.
Avoid High Temperature, Moisture, Water and Dust
This unit must not be exposed to dripping or splashing.
This unit is intended for indoor purposes only.
4.2 Electromagnetic and Other Interferences
All electronic devices, especially electronic equipment containing radio senders and/or receivers such
as mobile phones, computers, and antennas, give off electromagnetic emissions. This radiation is a
byproduct of electrical or magnetic activity. The emissions from such devices can interfere with other
devices, causing potential problems.
Equipment can be affected by electromagnetic interference from other devices in two major ways: The
rst is direct effects through proximity with other devices, and the second is electrical interference over
the power lines.
It is strongly recommended to:
• Make sure that all devices emitting electromagnetic radiation are kept a reasonable distance
away from the workstations in order to avoid any potential electromagnetic or other interferences.
• Have separate power circuits that are intended for use for medical equipment only.

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
4.3 Ambient Condition
In order to maintain operational safety and correct function of the equipment ensure that this unit is
installed at a location that meets the ambient conditions listed below:
• The temperature within the room must be between 20 °C-30 °C
• The relative humidity must not exceed 75% (non-condensing)
• The location must be equipped with appropriate ventilation system
• The unit must be kept away from heat generating devices
• The ooring of the location must be hard, non-ammable and at
• The power outlet should be out of casual reach to prevent accidental shut off
• Make sure that the device is correctly attached to ground using grounding-type plug
5 FEATURES
5.1 Laminar Flow
The L124 and the L126 IVF Workstations are supplied with two laminar ow modes:
• The Normal Mode must be used when working inside or nearby the laminar ow hood.
• The Standby Mode maintains the workstation interior under aseptic conditions, but working inside
the workstation or near the workstation may cause changes in the airow.
5.1.1 Flow Pattern Diagram

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
5.1.2 Pre-lter
Air entering the workstation is pre-ltered with an efciency
of 83%.
WARNING
Only use original K-SYSTEMS VOC
lters.
5.1.3 Fan
The air is drawn into the fan in the top of the workstation
where it is pressurized. From the pressure plenum the air
passes through the HEPA main lter.
5.1.4 Main Filter
The lter efciency of the HEPA main lter is 99.995% of particles 0.3 μm (D.O.P. test).
The air ows from the main lter through the work chamber in a vertical unidirectional ow of clean air.
Immediately before reaching the tabletop, the air separates and ows out through the back wall, and
through the work opening. The air returns to the suction opening of the workstation passing through the
surrounding space.

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
5.1.5 Gassing Station
Most of the culture media that is used in assisted reproduction
techniques is sensitive to changes in pH, it is important to ush the
media with CO2.
The pH of the media in the test tubes and dishes may be controlled
by ushing with appropriate gas mixtures.
The pre-mixed CO2gas supply is connected to the back of the IVF
Workstation. It is being heated and humidied by the gas washing
bottles placed in the inner wall of the IVF workstation and coming out
through the gassing nozzles built into the table plate. An incubator
hood should be placed above the nozzles at all times.
The ow rate can be adjusted as well as read at the display
in the inner wall
For optimal use, only distilled water should be used.
5.1.6 Gas Mixture
Follow indications from the culture media provider for correct handling of media. Culture media
must be maintained at a pH of about 7.4, this can be obtained by gassing with an atmosphere
of 5% CO2.
The most frequently used gas mixtures are 5% CO2-in-air, and 5% CO2, 5% O2and 90% N2.

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
5.2 Stereo Microscope
A stereo microscope with light source is installed in the
table plate. For the MP Version of the L100 Workstation,
an inverted microscope can also be installed.
Mount the microscope stand by placing the light pillar
on top of the table plate and insert the screws from
underneath the table plate.
5.3 Interior Light
The interior light provides overhead illumination.
5.4 Power Supply
The power supply is 220-240V/50 Hz or 110-115V/60 Hz.

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
5.5 Electrical Outlet
WARNING Do not put any metal parts or ngers into the power supply plug.
5.6 Front Window
Placement of clamps Picture of clamps
WARNING
Do not remove clamps fastened to the window. This can cause the window
to fall down.

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
6 KEYBOARD FUNCTIONS
K-SYSTEMS Microscope Light Source
Fan ON, Reduced Fan Speed, Fan OFF
Alarm, Disable/Enable Acoustic Alarm
Interior Light ON and OFF
Heated Surface ON and OFF
Set-Point Key
Adjust Set-Temperature
Keyboard Lock/Unlock
Hold both keys for 3 seconds to enter the menu or
exit the menu
Temperature Display: Change between left and right
on the display (L126 Dual or fully Heated)
Change between left and right camera
(L126 Dual and L126 MP)
Change between hour and temperature reading
Heat area ON/OFF (only L126 Dual or Fully Heated)
except

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
6.1 The Interior Light
ACTION KEY
Press the switch to turn the interior light ON, indicated by the yellow light.
Press again and the interior light turns OFF.
6.1.1 Operating the Laminar Air Flow
ACTION KEY
Activating the laminar ow.
Press one time and the fan will run at full speed, indicated by the green light
(normal model).
A ve digit number will run though the display showing the total hour count for the
HEPA lter.
Press one more and the fan will operate at reduces speed, indicated by the green light
ashing (standby mode).
Press one more time and the fan will switch OFF, and the time will be shown.
CAUTION
There will be an alarm when the fan has run for 17,000 hours, indicating that the
HEPA lter must be checked. When the alarm goes on the fan can still be used.
6.2 HEATING SYSTEM
6.2.1 Table Heating Unit
ACTION KEY
Press one time and the HEAT will turn ON. The display shows the actual table
temperature.
Press again and the HEAT will be turned OFF.
6.2.2 Setting the Temperature
ACTION KEY
Press the key with the symbol SP, the display will ash between the
SET-POINT temperature and the unit that the temperature is shown in.
Hold down the SP key and press the UP or DOWN key to change the
SET-POINT temperature. When the SET-POINT is set, release both
keys.

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
NOTE: If the SP key and the UP or DOWN keys are pressed continuously, the temperature setting will
change quickly. To change the temperature slowly, press one step at a time.
The L126 Dual can have separate SET-POINTS for each heated surface.
Press the DOWN key to change between right and left temperature readings.
Right Temperature Reading:
ACTION KEY
Press the SP key, and the display will ash between "r °C" / " r °F" and the
SET-POINT temperature. ("r °C" / " r °F" means the SET-POINT for the
right side).
Hold down the SP key and press the UP or DOWN key to change the
set-point temperature. When the SET-POINT is set, release both keys.
Left Temperature Reading:
ACTION KEY
Press the SP key, and the display will ash between "L °C" / " L °F" and the
SET-POINT temperature. ("L °C" / " L °F" means the SET-POINT for the left
side).
Hold down the SP key and press the UP or DOWN keys to change the
set-point temperature. When the SET-POINT is set release both keys.
NOTE: It is only possible to have two SET-POINTS if the SP function in the menu is set to "L-r".

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
6.2.3 Alarm
The workstations have an alarm that warns if the temperature is too high or too low. The
system also has an error mode alarm. The alarm is a visual red light on the keyboard display
with an audible pulsating alarm tone. The alarm will sound the rst time the unit is powered up
or after a power shortage. The alarm can be turned off by pushing the SP key.
When the heating system is rst activated, it will warm until the set-temperature has been reached.
During this period the alarm will not be activated.
The following messages can be shown on the display in case of alarm:
DISPLAY MESSAGE
Temperature alarm on Zone-1 (See Section 7: Different Heated
Surfaces).
Temperature alarm on Zone-2 (See Section 7: Different Heated
Surfaces).
Temperature alarm on Zone-3 (See Section 7: Different Heated
Surfaces).
Temperature alarm on Zone-L (See Section 7: Different Heated
Surfaces).
Temperature alarm on Zone-r (See Section 7: Different Heated
Surfaces).
Temperature alarm on Zone-4 (See Section 7: Different Heated
Surfaces).
Temperature alarm on Zone-5 (See Section 7: Different Heated
Surfaces).
Temperature alarm on Zone-6 (See Section 7: Different Heated
Surfaces).
Temperature alarm on the heat area (See Section 7: Different
Heated Surfaces).
Error mode: Reset the workstation by disconnecting the power
(sound can’t be muted).
NOTE: Warming up from 20 °C to 37 °C takes approximately 45 minutes without warming blocks on the
table. After placing an aluminum block on the table, it may take up to 30 minutes for the block
temperature to stabilize, depending on the initial block temperature.