Phcbi MDF-C2156VAN Series User manual

MDF-C2156VAN 
MDF-C2156VANCSeries
Please read the operating instructions carefully before using this product, and keep the operating
instructions for future use.
See page 40 for all model numbers.
Operating Instructions
Ultra-Low Temperature Freezer
MDF-C2156VAN

1
CONTENTS
INTRODUCTION P.2
PRECAUTIONS FOR SAFE OPERATION P. 3
ENVIRONMENTAL CONDITIONS P. 7
FREEZER COMPONENTS P. 8
Control panel P. 11
INSTALLATION SITE P. 12
INSTALLATION P. 13
START-UP OF UNIT P. 14
Operation after power failure P. 14
BASIC SCREEN OF CONTROL PANEL P. 15
FUNCTION OF CONTROL PANEL P. 15
RUNNING OPERATION (MENU/Set) P. 16
Key lock function P. 17
Key lock release function P. 17
VARIOUS SETTING (MENU/Log) P. 18
Display of log (Log) P. 18
PC setting P. 19
VARIOUS SETTING (MENU/Tools) P. 20
Initialization (Tools/ Default Setting) P. 20
Setting of date, time, log interval (Tools/Date Time) P. 21
Setting of key lock password (Tools/Key lock PW Setting) P. 22
ALALRM BUZZER P. 22
MONITOR OF FREEZER STATUS P. 23
ALARMS & SAFETY FUNCTIONS P. 23
REMOTE ALARM TERMINAL P. 25
ANALOG OUTPUT TERMINAL P. 25
BACKUP COOLING KIT P. 25
ROUTINE MAINTENANCE P. 27
Cleaning of cabinet P. 27
Cleaning of condenser filter P. 27
Defrosting of inside wall P. 28
REPLACEMENT OF BATTERY P. 29
TROUBLESHOOTING P. 30
DISPOSAL OF UNIT P. 31
Recycle of battery P. 31
TEMPERATURE RECORDER (OPTION) P. 36
MOUNTING OF INTERFACE BOARD (OPTION) P. 38
SPECIFICATIONS P. 39
PERFORMANCE P. 40
SAFETY CHECK SHEET P. 41

2
INTRODUCTION
■Read the operating instructions carefully before using the product and follow the instructions for safe
operation.
■PHC Corporation takes no responsibility for safety if the product is not used as intended or is used with
any procedures other than those given in the operating instructions.
■Keep the operating instructions in a suitable place so that they can be referred to as necessary.
■The operating instructions are subject to change without notice for improvement of performance or
function.
■Contact our sales representative or agent if any page of the operating instructions is lost or the page
order is incorrect, or if the instructions are unclear or inaccurate.
■No part of the operating instructions may be reproduced in any form without the express written
permission of PHC Corporation.
IMPORTANT NOTICE
PHC Corporation guarantees this product under certain warranty conditions. However, please note that
PHC Corporation shall not be responsible for any loss or damage to the contents of the product.

3
PRECAUTIONS FOR SAFE OPERATION
It is imperative that the user complies with the operating instructions
as it contains important safety advice.
Items and procedures are described so that you can use this unit correctly and safely.
If the precautions advised are followed, this will prevent possible injury to the user and
any other person.
Precautions are illustrated in the following way:
WARNING
Failure to observe WARNING signs could result in a hazard to personnel
possibly resulting in serious injury or death.
CAUTION
Failure to observe CAUTION signs could result in injury to personnel and
damage to the unit and associated property.
Symbol shows;
This symbol means caution.
This symbol means an action is prohibited.
This symbol means an instruction must be followed.
Be sure to keep the operating instructions in a place accessible to users of this unit.
< Label on the unit >
This mark is labeled on the cover in which the electrical components of high voltage
are enclosed to prevent the electric shock.
The cover should be removed by a qualified engineer or service personnel only.
WARNING
As with any equipment that uses N2gas, there is a likelihood of oxygen depletion in the vicinity
of the equipment. It is important that you assess the work site to ensure there is suitable and
sufficient ventilation. If restricted ventilation is suspected, then other methods of ensuring a
safe environment must be considered. These may include atmosphere monitoring and
warning devices.
For the State of California, USA Only:
This product contains a CR Coin Cell Lithium Battery which contains Perchlorate Material – special
handling may apply. See www.dtsc.ca.gov/hazardouswaste/perchlorate.

4
PRECAUTIONS FOR SAFE OPERATION
Do not use the unit outdoors. Current leakage or electric shock may result if the unit is exposed to
rain water.
Only qualified engineers or service personnel should install the unit. The installation by
unqualified personnel may cause electric shock or fire.
Install the unit on a sturdy floor and take an adequate precaution to prevent the unit from
turning over. If the floor is not strong enough or the installation site is not adequate, this may result
in injury from the unit falling or tipping over.
Never install the unit in a humid place or a place where it is likely to be splashed by water.
Deterioration of the insulation may result which could cause current leakage or electric shock.
Never install the unit in a flammable or volatile location. This may cause explosion or fire.
Never install the unit where acid or corrosive gases are present as current leakage or electric
shock may result due to corrosion.
Always ground (earth) the unit to prevent electric shock. If the power supply outlet is not
grounded, it will be necessary to install a ground by qualified engineers.
Never ground the unit through a gas pipe, water main, telephone line or lightning rod. Such
grounding may cause electric shock in the case of an incomplete circuit.
Connect the unit to a power source as indicated on the rating label attached to the unit. Use
of any other voltage or frequency other than that on the rating label may cause fire or electric shock.
Never store volatile or flammable substances in this unit if the cylinder cannot be sealed. These
may cause explosion or fire.
Do not insert metal objects such as a pin or a wire into any vent, gap or any outlet on the unit.
This may cause electric shock or injury by accidental contact with moving parts.
Use this unit in safe area when treating the poison, harmful or radiate articles. Improper use
may cause bad effect on your health or environment.
Turn off the power switch (if provided) and disconnect the power supply to the unit prior to any
repair or maintenance of the unit in order to prevent electric shock or injury.
Do not touch any electrical parts (such as power supply plug) or operate switches with a wet
hand. This may cause electric shock.
WARNING

5
PRECAUTIONS FOR SAFE OPERATION
Ensure you do not inhale or consume medication or aerosols from around the unit at the time of
maintenance. These may be harmful to your health.
Never splash water directly onto the unit as this may cause electric shock or short circuit.
Never put cylinders with liquid on the unit as this may cause electric shock or short circuit when
the liquid is spilled.
Never bind, process, or step on the power supply cord, or never damage or break the power
supply plug. A broken supply cord or plug may cause fire or electric shock.
Do not use the power supply cord if its power supply plug is loose. Such power supply cord
may cause fire or electric shock.
Never disassemble, repair, or modify the unit yourself. Any such work carried out by an
unauthorized person may result in fire, or electric shock or injury due to a malfunction.
Disconnect the power supply plug if there is something wrong with the unit. Continued
abnormal operation may cause electric shock or fire.
When removing the power supply plug from the power supply outlet, grip the power supply
plug, not the cord. Pulling the cord may result in electric shock or fire by short circuit.
Disconnect the power supply plug before moving the unit. Take care not to damage the power
supply cord. A damaged cord may cause electric shock or fire.
Disconnect the power supply plug when the unit is not used for long periods. Keeping the
connection may cause electric shock, current leakage, or fire due to the deterioration of insulation.
If the unit is to be stored unused in an unsupervised area for an extended period, ensure that
children do not have access and that doors cannot be closed completely.
The disposal of the unit should be accomplished by appropriate personnel. Remove doors to
prevent accidents such as suffocation.
Do not put the packing plastic bag within reach of children as suffocation may result.
Connect power supply cord of the product to its own dedicated outlet. Failure to observe this
caution may result in ignition or malfunctioning.
Do not position this unit and the other unit so that it is difficult to operate the disconnection of
the power supply plug. Failure to disconnect the power supply plug may cause fire if there is
something wrong with the unit.
WARNING

6
PRECAUTIONS FOR SAFE OPERATION
This unit must be plugged into a dedicated circuit protected by branch circuit breaker.
Use a dedicated power source as indicated on the rating label attached to the unit. A multiple-tap
may cause fire resulting from abnormal heating.
Never store corrosive substances such as acid or alkali in this unit if the cylinder cannot be sealed.
These may cause corrosion of inner components or electric parts.
Check the setting when starting up of operation after power failure or turning off of power
switch. The stored items may be damaged due to the change of setting.
Be careful not to tip over the unit during movement to prevent damage or injury.
Prepare a safety check sheet when you request any repair or maintenance for the safety of service
personnel.
CAUTION

7
ENVIRONMENTAL CONDITIONS
This equipment is designed to be safe at least under the following conditions (based on the IEC 61010-1):
■Indoor use;
■Altitude up to 2000 m;
■Ambient temperature 5 oC to 40 oC;
■Maximum relative humidity 80 % for temperature up to 31 o
C decreasing linearly to 50 % relative
humidity at 40 oC;
■Mains supply voltage fluctuations up to ±10 % of the nominal voltage;
■Transient overvoltages up to the levels of OVERVOLTAGE CATEGORY II;
■Temporary OVERVOLTAGES occurring on the mains supply;
■Applicable pollution degree of the intended environment (POLUTION DEGREE 2 in most cases).

8
FREEZER COMPONENTS
Rear side
Inside of side table
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9
FREEZER COMPONENTS
1. Lock: Turn clockwise to 180owith a key and the door is securely locked.
2. Door: Hinged type. The door can be opened in any angle on the way to full open.
3. Magnetic door gasket: Seals the door and prevents leakage of cold air.
4. Inner lid: Serves as a means of reducing cold air leakage when the door is open.
5. Access port: Serves a means of leading the measuring cable from the chamber to the outside.
6. Caster: 6 casters are provided. They make the moving of the unit easier.
7. Leveling foot: Serves to adjust the height and to settle the frame.
8. Grille: Acts as an inlet for air to cool the motor. Be careful not to block this. By pulling down this
grille, you can clean a clogged condenser filter. See page 27 for the details.
9. Exhaust air vent: Be careful not to block this.
10. Side table: The side table can be opened. There are access port and remote alarm cable output
port in the inside.
11. Panel door lock: To lock the control cover to avoid the setting by accidental contact.
12. Panel door: There is a controller such as a power switch inside the panel door.
13. Control panel: Refer to page 11.
14. Digital temperature indicator: This indicator shows the present temperature or setting
temperature.
15. Temperature recorder (OPTION): Refer to page 38.
16. Backup cooling kit joint: It is positioned at rear of the unit. Serves to connect with the pipelines
from the cylinder (liquid N2) at the top left of the rear frame. Refer to page 26 “Backup cooling kit”
17. Remote alarm cable output port
18. Backup test switch (TEST): Examine the functions of the backup cooling kit. This switch allows
liquid N2to spout under any circumstances. Handle it according to page 26 “Backup cooling kit”.
19. Backup switch (BACK UP): Switch on for operation of the backup cooling kit and switch off for
stopping. Handle this switch according to page 26 “Backup cooling kit”.
20. Communication box cover: Refer to “Mounting of interface board (OPTION)” on page 40 for
usage.

10
FREEZER COMPONENTS
21. Remote alarm switch (REMOTE ALARM): This switch is for remote alarm. Turn on this switch to
enable the remote alarm (function).
22. Remote alarm terminal (MAX DC 30 V 2 A)
23. Analog output terminal (ANALOG VOLTAGE)
24. Power switch (POWER): Power switch of the freezer.
25. Battery switch: It is the switch of the battery for the power failure alarm. Turn it on usually. Turn
it off when you do not drive the freezer for a long time. (More than 1 month)
26. Temperature control knob (TEMP. SET(oC)): It is the knob which adjusts operation temperature of
the backup cooling kit.
27. Side table handle: There is a lever at the center of the front. When it is pulled to the front, the side
table is open.

11
FREEZER COMPONENTS
Control panel
1. LCD panel
2. Battery check lamp (BATTERY): This lamp lights when the battery capacity decreases at the power
failure. Batteries are expendable supplies. Replace batteries every three years. For the replacement,
contact our sales representative or agent.
3. Alarm lamp (ALARM): This lamp flashes when the unit is in alarm condition.
4. Backup lamp (STANDBY): This lamp lights when the backup switch is on. (This doesn’t show
backup cooling kit is activated.)
5. Figure input key: User for operation setting.
6. Menu button (MENU): To open the menu window.
7. Shift key (Upward, downward, rightward, leftward): To move the cursor on the LCD panel.
8. Enter key (ENTER): To determine the selection of menu.
9. Clear key (CE): To clear the input value during setting.
10. Alarm buzzer stop key (BUZZER):
- Buzzer stop : Refer to page 22 for the details.
- Alarm test : Refer to page 15 for the details.
- Chamber temperature display : The chamber temperature is displayed by pressing this key during
power failure.
11. LCD contrast adjusting knob (CONT.): To adjust the contrast of LCD panel.
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oC Closed

12
INSTALLATION SITE
To operate this unit properly and to obtain maximum performance, install the unit in a location with the
following conditions:
■A location not subjected to direct sunlight
Do not install the unit under direct sunlight. Installation in a location subjected to direct sunlight cannot
obtain the intended performance.
■A location with adequate ventilation
Leave at least 10 cm around the unit for ventilation. Poor ventilation will result in a reduction of the
performance and consequently the failure.
■A location away from heat generating sources
Avoid installing the unit near heat-emitting appliances such as a heater or a boiler etc. Heat can
decrease the intended performance of the unit.
■A location with little temperature change
Install the unit under stable ambient temperature. The allowable ambient temperature is between 5 oC
and 30 oC.
■A location with a sturdy and level floor
Always install the unit on a sturdy and level floor. The uneven floor or tilted installation may cause failure
or injury. Install the unit in stable condition to avoid the vibration or noise. Unstable condition may
cause vibration or noise.
WARNING
Install the unit on a sturdy floor. If the floor is not strong enough or the installation site is not
adequate, this may result in injury from the unit falling or tipping over.
Select a level and sturdy floor for installation. This precaution will prevent the unit from tipping.
Improper installation may result in water spillage or injury from the unit tipping over.
■A location not prone to high humidity
Install the unit in the ambient of 80 %R.H. or less humidity. Installation under high humidity may cause
current leakage or electric shock.
WARNING
Do not use the unit outdoors. Current leakage or electric shock may result if the unit is exposed to
rain water.
Never install the unit in a humid place or a place where it is likely to be splashed by water.
Deterioration of the insulation may result which could cause current leakage or electric shock.
■A location without flammable or corrosive gas
Never install the unit in a location where it will be exposed to flammable or corrosive gas. This may
cause explosion or fire or may result in the current leakage or electric shock by the corrosion of the
electrical components.
■A location without the possibility of anything fall
Avoid installing the unit in the location where anything can fall down onto the unit. This may cause the
breakdown or failure of the unit.

13
INSTALLATION
1. Removing the packaging materials and tapes
Remove all transportation packaging materials and tapes. Open the doors and ventilate the unit. If the
outside panels are dirty, clean them with a diluted neutral dishwashing detergent. (Undiluted detergent
can damage the plastic components. For the dilution, refer to the instruction of the detergent.) After
the cleaning with the diluted detergent, always wipe it off with a wet cloth. Then wipe off the panels with
a dry cloth.
Note:
Remove the cable tie banding the power supply cord.
Prolonged banding may cause the corrosion of the cord
coating.
2. Adjusting the leveling foot
Stretch the leveling feet by rotating them to contact them to
the floor. Ensure the unit is installed horizontally.
3. Fixing the unit
Two fixtures are attached to the rear of the frame.
Fix the frame to the wall with these fixtures and rope or
chain.
4. Ground (earth)
The ground (earth) is for preventing the electric shock in the case of unexpected deterioration of the
electrical insulation. Always ground the unit at the time of installation.
WARNING
Use a power supply outlet with ground (earth) to prevent electric shock. If the power supply outlet is
not grounded, it is necessary to install a ground by qualified engineers.
Never ground the unit through a gas pipe, water main, telephone line or lightning rod. Such
grounding may cause electric shock in the case of an incomplete circuit.
Leveling foot

14
START-UP OF UNIT
Use the following procedure to start trial operation or actual operation of the unit.
1. Confirm that all switches (power switch, battery switch, remote alarm switch and backup switch) are off.
2. Set liquid N2cylinder.
3. Put the inner lid, close the door, and connect a power supply cord to the power supply outlet.
4. Turn on the power switch. The chamber temperature is indicated.
5. Set up the temperature with the temperature control knob when using a backup cooling kit.
6. Turn on the battery switch, remote alarm switch and backup switch.
7. Push the alarm buzzer stop key (BUZZER) for about 5 seconds, and confirm the flashing of the alarm
lamp and a buzzer sound. When it is pushed again, an alarm test movement is finished.
8. Push the backup test switch, and confirm that liquid N2flows into the freezer chamber.
9. Put the sample to cryopreserve into the freezer chamber. Turn off the remote alarm switch and the
backup switch when temperature of the sample is high. Then, turn on these switches under the
condition that a sample is refrigerated again.
Operation after power failure
The set value is memorized by nonvolatile memory. Accordingly, the freezer resumes the operation with
setting before power failure. During the power failure, the clock function is operating.
■When this product operates at the first start-up or after no use for long period, the built-in battery
capacity may be lowered or completely zero because of discharge of the battery. After installation the
product, the freezer should operate for more than three days (72 hours) to charge the battery.

15
BASIC SCREEN OF CONTROL PANEL
When the power switch is turned on, the basic screen of the control panel is indicated.
1. Display of alarm (Alarm): Normal condition: “Normal” is displayed.
Alarm-activated, buzzer-delayed: “Alarm” is displayed alternately in reversal/non-reversal character.
Alarm-activated, buzzer-sounding: “Warning” is displayed alternately in reversal/non-reversal character
The complementary message is indicated in the message indication.
●“Test” is displayed alternately by the reversal/non-reversal character during the alarm test.
2. Display of status (Status): Normal condition: “Stand-by” is displayed.
The status number is displayed and a complementary message is displayed in the message column when the
operation monitor system detects the specified status. Refer to page 23 for the details.
3. Display of door status (Door): “Open” is highlighted when the door is open. When the door is
close, "Closed" is displayed . (Normal character)
4. Display of setting (Temp): Set value of chamber temperature is displayed.
5. Display of present chamber temperature: Present value of chamber temperature is displayed.
6. Message indication: Various messages are indicated. Refer to page 23 and 24 for the details.
7. Display of date and time: The current date and time are displayed.
FUNCTION OF CONTROL PANEL
The following functions are available through control panel:
1. Setting of running operation: To set a running condition at the start-up. (refer to page 16)
2. Setting of log cycle and sending to PC: To set a log cycle of running data and to send a running
log to PC. (page 19)
3. Setting of date and time: To set the date and time shown on the basic screen. (page 21)
4. Setting/display of alarm: High limit (or low limit) temperature setting(page 16). Power failure alarm
(page 23) and filter alarm (page 23) are indicated.
5. Default setting: To set the default for LCD panel and communication (DAQ) speed etc. (page 20)
6. Alarm test: The test of alarm buzzer, alarm lamp and remote alarm is effective by pressing the alarm
buzzer stop key (BUZZER) for about five seconds during normal operation. Pressing the key again
finishes the alarm test.
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16
RUNNING OPERATION (MENU/Set)
This product is operated with set temperature at the time of start-up.
1. With the basic screen displayed, press the menu button (MENU) to show the menu window. Select
“Set”, and press the enter key (ENTER).
2. A temperature setup screen (Temp. Setting) is indicated. Set up each parameter.
3. Push the menu button (MENU) to finish the settlement of each parameter. Select OK on the menu
window, and push the enter key (ENTER). The setting is memorized.
Each parameter setting range:
●Temperature : It is the set point of chamber temperature. Temperature settable range : -125 oC~
-152 oC (The factory setting is -150 oC.)
●High Alarm: It is the set point of the high temperature alarm. Temperature settable range: Chamber
temperature +5 oC~+20 oC (The factory setting is +10 oC)
●Low Alarm: It is the set point of the low temperature alarm. Temperature settable range: Chamber
temperature -5 oC~-20 oC (The factory setting is -10 oC)
●Ring Back: This is the duration between the stop of alarm buzzer and next start of alarm buzzer. The
settable range is between 1 minute and 99 minutes. The alarm buzzer is not back again when the
setting is 0. (The factory setting is 30 minutes)
●Alarm Delay: The settable range of the alarm delay time is between 0 and 15 minutes. With 0 setting,
the alarm buzzer activates immediately without delay. (The factory setting is 15 minutes.)
●Key Lock: When "Lock" is chosen, a set point cannot be changed. The input of the password is
necessary at the time of release.
Note : An alarm buzzer operates 15 minutes after it became a state of alarm. When the door is open,
it is indicated on the basic screen (Door) by "Open" and the reversal character.

17
RUNNING OPERATION (MENU/Set)
Key lock function
1. On the temperature setting screen (Temp. setting), input 1 in the “Key Lock” field. Press the enter
key (ENTER) and the buzzer sounds shortly and the key lock is effective.
2. “Key Lock” is indicated on the top line. The change of setting is impossible.
Key lock release function
1. On the temperature setting screen (Temp. Setting), input 0 in the “Key Lock” field, and press the
enter key (ENTER).
2. The cursor is moved to the password column (Password). Input 4-digit password and press the
enter key (ENTER). The buzzer sounds shortly and the key lock is released. The “Key Lock” indication
of the temperature setting screen (Temp. Setting) disappears.
Note : The buzzer sounds if a password is wrong. Again, input a password. A user should manage a
password with all of the members. Factory setting is 0000. Refer to page 22.
1
Key Lock
1
Key Lock
Key Lock

18
VARIOUS SETTING (MENU/Log)
Display of log(Log)
1. On the basic screen, press the menu button (MENU) to show the menu window. Select log, and
press the enter key (ENTER).
2. When accumulated data (for one day) are transmitted to the PC: Press the menu (MENU), select “PC
1D” and press the enter key (ENTER). When all accumulated data are transmitted to the PC: Press the
menu button(MENU), select “PC ALL” and press the enter key (ENTER). All accumulated records are
indicated with a dot. A graph display (PC 1D) is indicated. (1 page 24-hour indication)
3. Data transmitting screen is indicated. Specify a transfer, a capture of the textbook and a preservation
file name by the operation on the PC side hyper-terminal. Specify the extension of the preservation file
name with txt or csv. Press the menu button (MENU), select “start” and press the enter key (ENTER).
The transmission is started. The transmission is finished when a finished message comes out.
Each parameter setting range:
●Temperature range is changed with ↓↑. Temperature :50 oC~-25 oC,-25 oC~-100 oC,-100 oC~-175 oC
●It is moved with ←and →to date. (←: Past date, →: New date)
●A data interval is 2 minutes from 30 minutes. (The factory setting is 15 min.)
With 15 minutes interval, the recording for about 5 weeks is available. With 6 minutes interval, the
recording for about 2 weeks is possible.
●Pop-up screen is indicated when “PC 1D” is selected. Log data on date (for 1 day) indicated.
●Pop-up screen is indicated when “PC ALL” is selected and all memory log data are transmitted.
●Pop-up screen is indicated when the clear key (CE) is pushed. All log data are erased when OK is
selected by pop-up MENU.
Note :
●When log data are full, it is erased automatically in order from the old data.
●The communication cable for the interface board MTR-480 of the option and 9 pin Dsub cross type for
RS232C is necessary for the data transmission to the PC. Refer to page 40 for the details.
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