QBC Diagnostics ParaLens User manual

ParaLens
ParaLens
QBC Diagnostics Inc.
200 Shadylane Drive
Philipsburg, Pennsylvania 16866 USA
4277-060-032 Rev A
SPECIFICATIONS
OptiLume Illuminator:
Input: 4 – 24 VDC; 0.7 – 2.5 Amps
Center positive DC power connector
Output: 30lm, 470nm
Environmental: Indoor or outdoor sheltered
Dimensions and weight: 1¼˝Diameter × 3⅛Long
(31.75 mm × 79.375 mm)
3.62 oz. (0.10254 kg )
Compliance: EU MDD 93/42/EEC
Power Supply:
Input: 100 – 240 V AC; 47 – 63 Hz, 0.4 A
Output: 9V DC, 1.4 A
Compliance: UL, CE
REPLACEMENT PARTS
Catalog Description
424275 ParaLens Adapter - 40X Dry Objective
424273 ParaLens Adapter - 60X Oil Objective
424276 ParaLens Adapter - 100X Oil Objective
427751 OptiLume Light Source
427708 Immersion Oil
427707 Screwdriver
427711 Power Pack w/ Adapters
424278 ParaViewer
427710 QBC Malaria Test Starter Kit
427712 OptiLume System Carrying Case
w/ Foam Insert
427713 OptiLume System Carrying Case
w/o Foam Insert
427714 Foam Insert for OptiLume System
Carrying Case
814-342-6205 • www.qbcdiagnostics.com
Fluorescence Microscopy System
Fluorescence Microscopy System
with OptiLume Light Source
with OptiLume Light Source

PRECAUTIONS & MAINTENANCE:
PRECAUTIONS:
USE ONLY THE POWER PACK (p/n 427711) THAT IS SUPPLIED WITH THE
PARALENS SYSTEM
Never open the ParaLens System – there are NO serviceable parts contained
with the ParaLens Adapter or OptiLume Light Source.
Allow system to cool before cleaning and storage.
Due to intense light, NEVER look into the bottom hole of the objective while
the OptiLume is on.
Handle the OptiLume as a laboratory instrument.
CLEANING ADAPTER AND OBJECTIVE:
This product utilizes a spring-loaded oil immersion objective. It is
recommended that the oil be removed after use. Wipe off excess oil
using lens tissue. Wash the surface with an alcohol solution. Use lens
tissue or a soft, lint-free cloth. Allow to air dry.
CLEANING PARAVIEWER:
Wipe the ParaViewer Tube Holder with soft, lint-free tissue. If further cleaning
is necessary, use a mild detergent and warm water.
DO NOT USE SOLVENTS such as Xylene, Acetone, etc., as these will
permanently damage the ParaViewer Tube Holder.
SERVICE ASSISTANCE:
In order to obtain assistance with test procedures and equipment operation,
contact your nearest QBC Diagnostics representative or office.
Do not return any equipment without specific instructions from QBC
Diagnostics.
Blood Parasite Morphology is available at www.qbcdiagnostics.com.
ADDITIONAL QBC PRODUCTS:
ParaLens Adapters:
424275 ParaLens Adapter - 40X Oil Objective
424276 ParaLens Adapter - 100X Oil Objective
253037 Malaria tubes (100 Pack)
Automated Hematology Equipment:
429000 QBC STAR
428605 QBC AutoRead System
423406 Accutubes (100 Pack)
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Table of Contents
Standard Included Equipment.............................2
ParaLens Adapter System
Introduction....................................................3
ParaLens Installation ....................................3
OptiLume Light Source Installation ..............3
Operation Procedure ....................................4
Parasite Examination Procedure .................4
Precautions and Maintenance
Precautions ...................................................5
Cleaning Adapter and Objective ..................5
Cleaning ParaViewer ...................................5
Service Assistance .......................................5
Additional QBC Products
ParaLens Adapters ......................................5
Automated Hematology Equipment ............5
Specifications ......................................................6
Replacement Parts .............................................6
OPERATION PROCEDURES:
The ParaLens System provides users with epi-fluorescence capability; however,
if using with a standard transmission light microscope the microscope’s light
source should be turned off to use the ParaLens System.
When the ParaLens Fluorescence Microscopy System is connected to a power
source, it will be “ON”. The intensity knob (Item 3, Figure 2) on the ParaLens
OptiLume Light Source controls the operating current and the light output of
the LED. Turning the knob clockwise will increase intensity. Turning the knob
counterclockwise will decrease intensity.
Handle the ParaLens Fluorescence Microscopy System as a laboratory
instrument.
To turn the ParaLens System “OFF”, unplug from power source.
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4.
PARASITE EXAMINATION PROCEDURES (With QBC Malaria Tubes):
REQUIRED MATERIALS:
ParaViewer (p/n 424278)
Standard Microscope equipped with a ParaLens UV Microscope Adapter (p/n
424280)
Fluorescence Optical Immersion Oil (p/n 427708)
QBC Malaria Tubes (100 Pack - p/n 253037) (2000 Pack - p/n 253005)
EXAMINATION PROCEDURES:
Insert the centrifuged QBC Malaria Tube into the groove of the ParaViewer
Tube Holder. Slide the tube forward in the groove and under the Retainer Bar
until the open end of the tube is against the Retainer Stop. This will prevent
movement of the tube when it is manually rotated during the microscopic
examination procedure in Step (5).
Place the ParaViewer Tube Holder with QBC Malaria Tube on the stage of
the microscope. Using 10X, wide-field, high-point eyepiece(s) and ParaLens
Adapter with objective lens, add 2-3 drops of fluorescence optical immersion
oil to top of tube while in ParaViewer. Bring the buffy coat area of the tube
into focus.
Move the microscope stage until
the top of the expanded RBC
layer (near the RBC-Granulocyte
interface) is in the field of view.
Examine the sample.
By means of the tube closure,
rotate and examine the entire
circumference of the tube as it
rests in the groove of the ParaViewer. (See package insert instructions with
QBC Malaria Tube for description and detection of parasites.)
Note: The QBC Malaria Tube may incorporate a small patient ID Label affixed between the white
lines on the tube. When properly positioned, the label should be within the cavity of the ParaViewer
so that it does not interfere with rotation of the tube in the ParaViewer groove.
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200 Shadylane Drive
Philipsburg, PA 16866
814-342-6205 (phone)
814-342-6211 (fax)
www.qbcdiagnostics.com

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TYPE B
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STANDARD INCLUDED EQUIPMENT:
3
p/n: 427751 p/n: 424273 p/n: 427711
p/n: 427708
p/n: 424278 p/n: 427707
p/n: 427710
ParaLens OptiLume
Light Source
ParaLens Adapter w/ Objective
Power Pack w/ Adapters
Immersion Oil
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2.
3.
4.
ParaViewer
Screwdriver
Malaria Tube Starter Kit
(Stored in compartment under
Immersion Oil and ParaViewer)
5.
6.
7.
Also included (not shown)
OptiLume System Carrying Case
w/ Foam Inserts (p/n 427712)
Literature Packet (p/n 427709)
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PARALENS ADAPTER SYSTEMS:
INTRODUCTION:
The ParaLens is a complete epi-fluorescence microscopy system. Contained
within the system are the necessary objectives, filter, and beamsplitter
for use during fluorescent microscopy. The standard configuration of the
objective is appropriate for ∞tube length microscopes.
,CAUTION:
The ParaLens System uses a Class 2 LED product. Never stare into
OptiLume Light Source or adapter when ParaLens is turned “ON”
FIGURE 1. ParaLens Adapter w/ Objective
Adapter Body
Threads
Objective
Light Input Shaft
Set Screw
Thumbscrew
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6
2
5
1
3
4
1) Thumbscrew
2) Power pack
insertion point
3) Intensity knob
FIGURE 2. OptiLume Light Source
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2
PARALENS INSTALLATION PROCEDURE:
The ParaLens Adapter – see
figure 1 below – is equipped with
a threaded end (Item 2) that turns
freely within the ParaLens body
(Item 1). To install ParaLens
onto a standard microscope
begin by aligning the threaded
end of the ParaLens Adapter
with threaded screw holes in
the microscope nosepiece. Turn ParaLens Adapter clockwise to engage threads
on microscope nosepiece. Continue until ParaLens Adapter is securely fastened to
nosepiece of microscope.
The ParaLens Adapter may be rotated to any desired position and secured at given
location by tightening the set screw (Item 5) with provided ParaLens screwdriver. It is
not necessary to tighten the set screw for the ParaLens to function properly.
The ParaLens OptiLume Light Source must now be attached to the ParaLens Adapter.
OPTILUME LIGHT SOURCE INSTALLATION PROCEDURE:
Begin by removing the ParaLens Adapter thumb screw (Item 6, Figure 1).
Slide the ParaLens OptiLume Light Source onto the
ParaLens Adapter.
Tighten thumb screw (Item 1, Figure 2) on ParaLens
Optilume Light Source to secure the OptiLume Light
Source onto the ParaLens Adapter.
If using power pack, select proper wall adapter
and connect to power pack by aligning the tabs on
the adapter with the slot on the power pack. Push
together and slide downward. Power pack and
adapter are connected when a “snap” is heard.
Plug power pack into ParaLens Optilume light source
at insertion point (Item 2, Figure 2).
Plug power pack into an approved electrical
outlet. Be sure that outlet is always within reach as line cord is the means of
emergency disconnection.
The ParaLens Fluorescence Microscopy System should now be completely installed.
A blue light should now be visible below the ParaLens objective.
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