scinco Mega-800 User manual

5.
Mega-800 UV-Vis Spectrophotometer
Instruction Manual
September 2016

Instruction Manual
1Mega-800 SCINCO
Introduction
Thanks for your purchase of the SCINCO CO., LTD. Mega-800 spectrophotometer.
UV-Visible Spectrophotometer is a well-accepted, documented technique with many applications.
The technique is extensively used for the analysis of agricultural products and is widely used in public
health, environmental protection, life sciences industries and many other organic and biochemical
applications. SCINCO CO., LTD. developed its latest spectrophotometer Mega-800 based on its
proven technology. The advanced mould design ensures the outstanding quality, excellent
specifications and stable performance, which can fully meet the requirements of the analyzer.
For obtaining maximum performance and trouble-free operation, we give you the following suggestions:
Upon receiving your instrument, please check all the items listed in the packing list are included
and install the instrument according to the instruction manual.
The spectrophotometer is a precision electronic instrument. Please read the instruction
manual carefully before operation. If the equipment is used in a manner not specified by the
manufacturer, the protection provided by the equipment may be impaired.
Avoid collision and handle the instrument carefully during transportation.
If the tested solution is harmful to humans, please wear gloves or use other protection methods.
Please keep the accessories in a safe place for the use in future maintenance.
For more information, please visit www.scinco.com

2Mega-800 SCINCO
Contents
CHAPTER 1 Introduction .......................................................................................................................1
1.1 Unpacking ..................................................................................................................................1
1.2 Components description............................................................................................................2
1.2.1 Front view ................................................................................................................................2
1.2.2 Rear view .................................................................................................................................3
1.2.3 Side view .................................................................................................................................3
1.2.4 Display .....................................................................................................................................4
1.2.5 Keypad.....................................................................................................................................5
1.2.6 Sample cell holder ...................................................................................................................6
1.3 Specifications ............................................................................................................................8
CHAPTER 2 Installation .........................................................................................................................9
2.1 Site requirement .......................................................................................................................9
2.2 Installation .................................................................................................................................9
2.2.1 Connect the instrument to the printer.....................................................................................9
2.2.2 Connect the instrument to PC................................................................................................10
2.3 Turning on the instrument ......................................................................................................11
2.4 Performance check .................................................................................................................12
2.4.1 Wavelength accuracy and reproducibility ..............................................................................12
2.4.2 Baseline flatness ....................................................................................................................13
CHAPTER 3 Photometric measurement ............................................................................................14
3.1 Introduction..............................................................................................................................13
3.1 Gain access to photometric mode .........................................................................................14
3.2 Parameters setting ..................................................................................................................15
3.2.1 Set photometric mode ...........................................................................................................16
3.2.2 Set measuring wavelength .....................................................................................................16
3.2.3 Set K factor ............................................................................................................................18
3.2.4 Dark current correction ..........................................................................................................18
3.2.5 Quit parameters setting .........................................................................................................19
3.3 Sample control ........................................................................................................................19
3.3.1 Select sample module ............................................................................................................20
3.3.2 Set sample cell number in use (Drive Cell No.) ....................................................................20
3.3.3 No.1 cell blank correction ......................................................................................................21
3.3.4 Sample cell shifting (Move cell) ............................................................................................22
3.3.5 Reset to cell No.1 (Cell reset) ...............................................................................................22
3.3.6 Single cell measurement .......................................................................................................22
3.3.7 Quit sample control ................................................................................................................22
3.4 Auto zero (100T% correction) ................................................................................................22
3.5 Measurement ...........................................................................................................................22
3.5.1 Use single cell .........................................................................................................................22
3.5.2 Use multi-cells ........................................................................................................................23
3.6 Looking up the measurement results ....................................................................................24
3.7 Delete the measurement data .................................................................................................24
3.8 Printout .....................................................................................................................................25
3.9 Quit measurement ...................................................................................................................25
3.10 Application example ..............................................................................................................25

Instruction Manual
3Mega-800 SCINCO
CHAPTER 4 Spectrum scanning .........................................................................................................27
4.1 Gain access to spectrum mode ..............................................................................................27
4.2 Parameters setting ...................................................................................................................28
4.2.1 Set photometric mode .............................................................................................................29
4.2.2 Set scanning speed ................................................................................................................29
4.2.3 Set sampling interval ..............................................................................................................30
4.2.4 Set wavelength range .............................................................................................................31
4.2.5 Set ordinate range..................................................................................................................32
4.2.6 Light source selection.............................................................................................................33
4.2.7 Dark current correction............................................................................................................33
4.2.8 Quit parameters setting...........................................................................................................34
4.3 Baseline correction ..................................................................................................................34
4.4 Measurement ............................................................................................................................34
4.5 Peak picking ............................................................................................................................35
4.6 Sample control ........................................................................................................................36
4.7 Printout ....................................................................................................................................37
4.8 Quit measurement ..................................................................................................................37
4.9 Application example ................................................................................................................37
CHAPTER 5 Quantitative measurement.............................................................................................39
5.1 Gain access to quantitation mode .........................................................................................39
5.2 Parameters setting ..................................................................................................................40
5.2.1 Set measurement method ......................................................................................................41
5.2.2 Set measuring wavelength......................................................................................................41
5.2.3 Set concentration unit .............................................................................................................43
5.2.4 Dark current correction ..........................................................................................................43
5.2.5 Quit parameters setting ..........................................................................................................44
5.3 Construct the calibration curve .............................................................................................44
5.3.1 K-Factor method ....................................................................................................................44
5.3.2 Standards method ..................................................................................................................46
5.4 Sample control ........................................................................................................................50
5.5 Auto zero ..................................................................................................................................50
5.6 Measurement ...........................................................................................................................50
5.6.1 Use single cell ........................................................................................................................50
5.6.2 Use multi-cells .......................................................................................................................51
5.7 Looking up the measurement results ....................................................................................52
5.8 Delete the measurement data ................................................................................................52
5.9 Printout ....................................................................................................................................52
5.10 Quit measurement . ..............................................................................................................52
5.11 Application example .............................................................................................................53
CHAPTER 6 DNA/Protein Analysis ....................................................................................................56
6.1 Daily Care.................................................................................................................................27
6.1 The principle of Protein/DNA analysis ..................................................................................56
6.1.1 Principle of using absorbance of UV light to determine protein .............................................56
6.1.2 Principle of using absorbance of UV light to determine DNA .................................................56
6.1.3 Protein/DNA determination methods .....................................................................................56
6.2 Gain access to DNA/Protein analysis ...................................................................................57
6.3 Parameters setting ..................................................................................................................59
6.3.1 Selection analysis method ....................................................................................................59

4Mega-800 SCINCO
6.3.2 Dark current correction .........................................................................................................60
6.4 Custom defined method .........................................................................................................61
6.4.1 Set measuring wavelength .....................................................................................................61
6.4.2 Set DNA factors .....................................................................................................................62
6.4.3 Set protein factors...................................................................................................................63
6.4.4 Set background correction at 320nm ....................................................................................63
6.4.5 Set calibration factors ............................................................................................................64
6.5 Auto zero ..................................................................................................................................65
6.6 Measurement ...........................................................................................................................65
6.7 Look up the measurement results ........................................................................................65
6.8 Delete the measurement data ...............................................................................................66
6.9 Printout ....................................................................................................................................66
6.10 Quit measurement ................................................................................................................66
CHAPTER 7 Utilities..............................................................................................................................67
7.1 Gain access to utilities ........................................................................................................67
7.2 Set lamp changeover wavelength..........................................................................................67
7.3 Set spectral bandwidth ..........................................................................................................68
7.4 Set tungsten lamp On/Off .......................................................................................................68
7.5 Set deuterium lamp On/Off ....................................................................................................69
7.6 Set control mode ......................................................................................................................69
7.7 Wavelength correction............................................................................................................70
7.8 Set time ....................................................................................................................................71
7.9 Reset system ............................................................................................................................71
CHAPTER 8 Maintenance ....................................................................................................................73
8.1 Points for attention ................................................................................................................73
8.2 Daily care ...............................................................................................................................73
8.3 Trouble shooting and maintenance ....................................................................................74
APPENDIX 1 Light source replacement .............................................................................................76
Replace the tungsten lamp ...............................................................................................................76
Deuterium lamp replacement ............................................................................................................77
APPENDIX 2 Cell holder replacement ................................................................................................80
APPENDIX 3 Fuse replacement ..........................................................................................................82

Instruction Manual
5Mega-800 SCINCO
Announcement
This manual contains copyrighted and proprietary information. No part of this publication may be
reproduced, or transmitted in any form by any means, electronic, mechanical, photocopying, recording or
otherwise, without the permission of SCINCO CO., LTD.
Information in this publication is subject to change without notice, as SCINCO CO., LTD. continues to
develop the latest technologies and products for our customers.
The units described in this manual are designed to be operated only by professionals.
Any errors or omissions that may have occurred in this publication despite the utmost care taken in its
production will be corrected as soon as possible, but not necessarily upon detection.

6Mega-800 SCINCO
Chapter 1 Introduction
1.1 Unpacking
When unpacking, check with the packing list to confirm that all the items listed are included. Should any
shortage or damage occur, contact the seller immediately. Refer to the following table 1-1 for the
standard instrument set. But refer to packing list for your configuration.
Table 1-1 Standard set
No.
Name
Amount
01
Main unit of UV VIS
Spectrophotometer
1 unit
02
Quartz cell
1 pair
03
Tungsten lamp
2 pcs
04
Plus screwdriver
2 pcs
05
Plain screwdriver
2 pcs
06
Fuse (2A、0.5A)
2 pcs each
07
Socket screw wrench
1pc
08
Power cord
1 pc
09
Instruction manual
1pc
10
Packing list
2 pcs

Instruction Manual
7Mega-800 SCINCO

8Mega-800 SCINCO
1.2 Components description
When unpacking, the instrument is present as shown in Fig. 1.1.
Fig. 1.1 Instrument overview
1.2.1 Front view
The front view is shown as Fig. 1.2.
Fig. 1.2 Front view
①Instrument cover
②Front baffle of sample compartment—standard front baffle for sample compartment. It should
be replaced when using some accessories.
③Chassis—The steel made chassis can greatly reduce vibration, and ensures the credibility of
the instrument.
④Instrument label
⑤Keypad and display—320×240 dots high resolution LCD and 27 keystrokes.
⑥Sample compartment cover—Open the cover to gain access to the sample compartment.

9Mega-800 SCINCO
1.2.2 Rear view
The rear view is shown as Fig.1.3.
Fig. 1.3 Rear view
①Light source house cover—Open the cover to gain access to the light source house, where
you can replace the deuterium lamp and tungsten lamp.
②Foot packing—Shockproof rubber washer is used.
③Printer interface—Connect to the HP parallel inkjet printers.
④RS-232 interface—Communicate with PC.
⑤Wiring terminal—Connect to ground wire.
⑥Outlet—Connect to power supply: AC 220V, 50Hz.
⑦Fuse—Can be replaced (refer to “Appendix 3 fuse replacement”).
⑧Power switch
⑨Power label—Describe the power requirements.
⑩Instrument nameplate
⑪Fan
1.2.3 Side view
Side view is shown as Fig.1.4. There is no other interfaces or labels except the ventilation grills.