Supelco Spectroquant Prove Series User manual

Quick Guide
Spectroquant®Prove
Spectroquant®Prove
Spectrophotometer 100 ●300 ●600

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Contents
1 Safety ........................................................................ 3
1.1 Intended use........................................................................................................... 3
2 Overview ................................................................... 4
2.1 Scope of delivery ..................................................................................................... 4
2.2 Overview of the instrument ....................................................................................... 4
2.3 Display and user interface......................................................................................... 5
3 Getting started ........................................................ 11
3.1 General notes on handling....................................................................................... 11
3.2 Initial setup .......................................................................................................... 11
3.2.1 Connecting the power supply .......................................................................... 11
3.2.2 First power-on .............................................................................................. 12
3.2.3 Language setup ............................................................................................ 13
3.2.4 Date,timeandcountry-specicsettings ........................................................... 13
3.2.5 Self-test....................................................................................................... 14
4 Operation................................................................. 15
4.1 Switchingthespectrophotometeronoro ................................................................ 15
4.2 System setup ....................................................................................................... 17
4.3 Zero adjustment for preprogrammed methods ........................................................... 18
4.3.1 Notes on zero adjustment .............................................................................. 19
4.3.2 When to repeat the zero adjustment? .............................................................. 19
4.4 Measurements....................................................................................................... 20
4.4.1 Performing a measurement............................................................................. 21
5 Appendix ................................................................. 23
5.1 Certicates ........................................................................................................... 23
5.2 Warranty .............................................................................................................. 24
5.3 Accessories ........................................................................................................... 25
For more information about the Spectroquant®Prove
please visit:
www.sigmaaldrich.com/spectroquant
For our Operating Manual please visit:
www.sigmaaldrich.com/spectroquant
For more information about the technical videos please
visit:
www.sigmaaldrich.com/photometry

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1 Safety
This Quick Guide contains basic instructions
that you must follow during the commissioning,
operation and maintenance of the spectropho-
tometer. Consequently, all responsible person-
nel must read this Quick Guide carefully before
working with the meter. Keep this Quick Guide in
the vicinity of the meter.
This is a class A device. This equipment may
cause interference in a residential installation. In
this case the user is encouraged to perform ap-
propriate measures to correct the interference.
Symbols Description
WARNING
Hazardous area (general). The xenon lamp (UV/VIS) emits radiation in the ultraviolet regi-
on, which may cause damage to the eyes. Never look directly in the radiation of this light
source without wearing proper eye protection. Protect your skin from the direct exposure
to UV light.
WARNING
Dangerous electrical voltage.
WARNING WARNING
Signiesinstructionsthatmustbefollowedpreciselyinordertopreventseriousdangers
to personnel.
CAUTION CAUTION
Signiesinstructionsthatmustbefollowedpreciselyinordertoavoidminorinjuriesto
personnel or damage to the instrument or the environment.
CAUTION CAUTION
This is a cautionary notice with a warning symbol drawing your attention to the risk of
(limited) harm to personnel.
NOTE NOTE
Signiesanoticedrawingyourattentiontospecialcharacteristics.
REFERENCE
Used to indicate references to other documents.
Please pay attention to the separate safety instructions leaflet (part of delivery scope) and read it
carefully.
Safety instructions
Safety instructions in this operating manual are
indicated by the warning symbol (triangle) in the
left margin. The signal word (such as "CAUTION")
indicates the danger level.
The following warning symbols are used:
1.1 Intended Use
The intended use of the spectrophotometer con-
sists exclusively of the carrying out of photomet-
ric measurements according to this operating
manual. Observe the technical specifications of
the cells in the operating manual.
Any other use is considered to be unauthorized.
The spectrophotometer was developed for per-
forming water analyses in the laboratory.

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2 Overview
2.2 Overview of the Instrument
Packaging
The spectrophotometer is shipped in protective
transport packaging.
CAUTION
Retain the original packaging including the inner
packaging to protect the instrument against hard
knocks if it has to be transported. Please note
that damage caused by improper transport voids
all warranty claims.
1Display and user
interface
2Flip-up cover
2.1 Scope of Delivery
•Spectrophotometer
•Power adapter
•Power connectors (3 pieces)
•Dust cover
•Zero cell
•Quick Guide (A4 format)
•Safety instructions
•Certificate of final inspection
3Shaft for rectangular
cells
4Shaft for round cells
Front of the instrument

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2 Overview – 2.2 Overview of the Instrument – 2.3 Display and User Interface
2.3 Display and User Interface
NOTE
The entire display is touch-sensitive. Make selec-
tions using a fingertip or special touch pen. Do
not touch the display with sharp objects (e.g. the
tip of a ballpoint pen).
•Do not place objects on the display, as doing
so may scratch it
•Touch buttons, words or symbols to select
them
•Scrollbars are provided to assist quick move-
ment through long lists
•Touch the arrow in the scrollbar to scroll up-
wards or downwards through the list
•Following selection, the item is activated im-
mediately
•Touching a main button outlines it in blue
•Selecting an item inverts it (with dark text be-
ing shown on a light background)
•Selecting a text inverts it (with dark text
being shown on a light background), e.g.
method-specific settings for concentration
mode "Show Absorbance"
•"0" is OFF, "I" is ON – the active selection is
displayed light grey with dark figure, in this
case the Show Absorbance is ON
Ports at the rear of the instrument
5Socket for plug-in power supply unit
6LAN port
7USB Mini B port
8USB-A ports
NOTE
All connections comply with SELV.
5678
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2 Overview – 2.3 Display and User Interface
Methods
List
Methods
Settings
Ad hoc
AQA
Main menu navigation
The main menu is always visible on the left: It
consist of two pages with four smart icons each.
To switch between the two pages push at
the bottom on the left.
Results List
System
Setup
Login/Log-
out
Timer
NOTE
The menu selected is always outlined in
blue.
NOTE
Action buttons like "Start", "Save", "Print" give
the following touch feedback:
Normal
Remains static
Active fields are always shown in bright color.
Pressed fields invert the color as long as the
chosen action is performed.
Disabled
Draws 30 % of the normal state
Inactive, disabled fields show faint color.
"Methods" and "Results" are the most often used
modes and they are at the top of the main menu
navigation.
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To leave these, the submenu has to be closed
by touching the main menu button again, in this
case:
NOTE
The main menus "Settings (Method Settings)",
"Ad hoc", "AQA", "System (Instrument Settings)",
"Login/Logout", "Timer" open up a submenu.
Example "Settings":
The main menu "Methods" comprises two main overview panels arranged as shown below:
the Concentration Measurement Overview and the Method List Overview.
Screen layout concentration measurement overview
12 Main
menu but-
tons
11 Main
menu
selection
button:
Switch be-
tween the
two main
menu
overviews
1Menu
title
2Alerts
8Info bar with
Information
button
9Notification symbols/
settings
6Citation
5Results
7Action
buttons
3Time-
stamp
4Measure-
ment range
10 Brief infor-
mation
2 Overview – 2.3 Display and User Interface

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2 Overview – 2.3 Display and User Interface
Screen layout method list overview
1Menu
title
6Name of
method
7Focuses
selected
main menu
button
4Selection
buttons
3Dropdown
box
(closed)
2Dropdown
box
(closed)
5Scrollbar

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2 Overview – 2.3 Display and User Interface
Buttons Description
Method list
List of all methods, irrespective of mode
Settings
Thisbuttonisusedtoactivatemethod-specicsettings
(e.g. sample dilution, turbidity correction, zero adjustment, sample blank, reagent blank)
Ad hoc
For performing measurements (absorbance/transmission, spectrum, kinetics)
Allows measurements to be performed without the need to create methods
Absorbance/Transmission Mode
Ad hoc submenu: perform absorbance or transmission measurements
Spectrum Mode
Ad hoc submenu: record spectrum
Method list: create methods -> Spectrum Mode
Kinetic Mode
Ad hoc submenu: perform kinetic measurement
Method list: create methods -> Kinetic Mode
AQA
Overview and list of all Analytical Quality Assurance (AQA) modes
AQA Status 1&2
AQA submenus: Status display of the period of validity and the outcome (passed/failed)
AQA1
AQA submenu: List of AQA1 methods
AQA2
AQA submenu: List of AQA2 methods
Pipette check
AQA submenu: List of pipette-checking methods
Result list
List of all stored results
System setup
This button is for optional instrument settings (e.g. date, time, updates etc.)
Login/logout
Log users in and out
Timer list
List of stopwatch functions
Overview of main buttons

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Action & Selection
Buttons Description
Start button
Start an action (e.g. measurement)
Start zero
Start zero adjustment for a method
Apply
Save
Stop
Close
Logout
User logout
Search method
Search/results list
Search function, search criterion: method name, method number or item number
Filter cancellation button
Cancelallsetlteroptions
Edit
For editing parameters
Create method
Print
Print to pdf (USB device) or printer
Export button
Allselectedresultsareexportedtoanexternalmemorydeviceas.csvle
Import button
Updates/Methods are imported from an external memory device into the instru-
ment
Delete
The selected items are deleted
Overview of action & selection buttons

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3 Getting Started
3.1 General Notes on Handling
The Spectroquant®Prove spectrophotometer is
an optical precision instrument. It should always
be handled with care, especially when in mobile
use. Always protect the instrument from condi-
tions that could damage the mechanical, optical
and electrical components. Please note the fol-
lowing in particular:
•The temperature and humidity during opera-
tion and storage must be within the limits
specified in the "Technical Data" section (see
the Operating Manual)
The instrument must never be exposed to
the following:
•Extreme dust, humidity and moisture
•Intense light and heat
•Fumes that are corrosive or contain high con-
centrations of solvents
In addition take care of the following:
•For measuring, the instrument must be placed
on a flat surface
•Spilled liquid or other material should be re-
moved immediately (see the Operating
Manual)
•If a cell has broken in the cell holder, the cell
holder should be cleaned immediately (see the
Operating Manual)
•The cover should always be closed when the
spectrophotometer is not in use
•When the spectrophotometer is being trans-
ported, the cell compartment must be empty
3.2 Initial Setup
Proceed as follows:
•Connect the power adapter (see section 3.2.1)
•Switch on the spectrophotometer (see section
3.2.2)
•Set the language (see section 3.2.3)
•Set the date and time (see section 3.2.4)
•Run the self-test (see section 3.2.5)
NOTE
For our Operating Manual please visit:
www.sigmaaldrich.com/spectroquant
For more information about the technical videos
please visit:
www.sigmaaldrich.com/photometry
3.2.1 Connecting the Power Supply
Power is supplied through the power adapter
provided. The power adapter supplies the spec-
trophotometer with the required voltage and
type of current (24 V DC).
CAUTION
The line voltage at the user location must fulfil
the specifications stated on the power adapter
(the specifications are also indicated in the Op-
erating Manual). Only ever use the 24 V power
adapter provided. Please note that damage
caused by using a different power adapter than
the one supplied voids all warranty claims.

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3 Getting Started – 3.2 Initial Setup
Connecting the power adapter:
1. Connect the miniplug 1of the power adapter
to the socket 2of the spectrophotometer.
2. Connect the power adapter to a wall socket.
3.2.2 First Power-on
After switching on the spectrophotometer for the
first time you are automatically guided through
the language, date and time setup procedures.
1. Press the ON/OFF button 1. The spectro-
photometer gives an audible signal (beep)
and starts booting for approximately 2 min-
utes. You will see the following display:
2. The display switches to language setup (see
section 3.2.3).
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3 Getting Started – 3.2 Initial Setup
3.2.3 Language Setup
The software supports several languages. When
you switch on the spectrophotometer for the
first time, a list of language options is automati-
cally displayed after the boot procedure.
1. Select the desired language 1.
2. Tap on the Save button 2to confirm.
NOTE
The saving process of changing the language re-
quires some seconds.
3.2.4 Date, Time and Country-specific
Settings
During initial setup, having set the language op-
tion you are automatically guided through the
date and time setup procedure.
1. Tap on the Date format button 1.
2. The calendar view pops up 2. You can now
enter the date.
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3. Tap on OK 3to confirm.
4. Tap on the Arrow button 4to choose the
country-specific basic date setting. The date
format can be set and displayed for EU and
US.
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3 Getting Started – 3.2 Initial Setup
5. Tap on the Time format button 5. The nu-
meric key panel 6pops up. Now you can
enter the time.
6. Tap on OK 7to confirm.
7. Tap on the Arrow button 8to choose the
country-specific basic time setting. The time
format can be set and displayed for EU and
US.
8. Tap on the Arrow button 9to choose the
decimal separator "."/"," used in your coun-
tr y.
9. Tap on the Save button 10 to confirm.
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3.2.5 Self-test
Following language, date and time setup the
spectrophotometer performs a self-test.
1. Remove all cells and close the cell compart-
ment cover.
2. Start the self-test with the Start button 1.
3. The spectrophotometer performs the self-
test.
Self-test
The self-test covers:
•Checks on memory, processor, internal inter-
faces, filter and lamp
•A calibration of the wavelength
When the self-test has ended, the display shows
the main menu.
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4 Operation
4.1 Switching the Spectrophoto-
meter On or Off
Switching on
1. Press the ON/OFF button 1. The spectro-
photometer gives an audible signal (beep)
and starts booting for approximately 2 min-
utes. You will see the following display: 4. Start the self-test with the Start button 2.
5. The spectrophotometer performs the self-
test.
Self-test
The self-test covers:
•Checks on memory, processor, internal inter-
faces, filter and lamp
•A calibration of the wavelength
When the self-test has ended, the display shows
the main menu.
Starting the self-test
3. Remove all cells and close the cell compart-
ment cover.
2. After the booting process the screen shows
the self-test dialog.
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The spectrophotometer automatically switches
off the backlight of the display 3when no but-
ton has been tapped within a period of 10 min-
utes. The backlight is switched on again with the
next tap.
The button functions are activated only following
a further tap.
NOTE
You can set a user-defined time for this function
(please see the Operating Manual for detailed in-
structions).
Switching off
Press the ON/OFF button 4to switch the spectro-
photometer off.
Energy-saving mode – display
NOTE
The instrument has an Auto-Power-Off function,
which switches it automatically off after a user-
defined time. This function is not active out of
the box, but you can turn it on in "System (In-
strument settings)".
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4 Operation – 4.1 Switching the Spectrophotometer On or Off
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4 Operation – 4.2 System Setup
Buttons Description
Information
This submenu displays the following information about the device:
Software/method versions, device class, lamp counter and serial number
Interface
This submenu displays the following settings options and standard settings:
Audible signals – ON, Backlight – 100 %, Print to pdf – ON
Region
This submenu displays the following settings options and standard settings:
Language, date, time and country zone EU/US, decimal separator – "."/"," (dot or comma)
Quality
This submenu displays the following settings options and standard settings:
Quick zero – OFF, AQA1 and AQA2 lock – OFF, Zero Adjustment expiry – ON (interval:
7 days), Use expired reagents – OFF, Service reminder – ON
Automation (Setup 4)
This submenu displays the following settings options and standard settings:
Energy saving mode – ON (10 minutes), AutoPowero–OFF, Autologo–OFF,
Auto store – ON, Auto print – OFF, Sample ID popup – OFF
User management
This submenu displays the following settings options and standard settings:
Activation of user management and administrator settings, User login required – OFF
Service
This submenu displays the following settings options:
Various service functions such as backup, restore, export of log or system data and
import of methods
Update
This submenu displays the option for performing software and method updates
4.2 System Setup
General instrument setup is carried
out in the "System" menu.

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4 Operation – 4.2 System Setup –4.3 Zero Adjustment for preprogrammed methods
Buttons Description
Network
This submenu displays the setting options for connecting the Prove device with a network
Prove Connect
This submenu displays the settings options for connecting the Prove device with the Prove
Connect software (the Prove Connect software is optionally available, order Nos. Prove
Connect to LIMS Y110860001 and Prove Connect to Dashboard Y110850001)
Please see the Operating Manual for detailed information on the buttons and their functions.
4.3 Zero Adjustment for
Preprogrammed Methods
A zero adjustment must be performed for each
cell type. The zero adjustment for concentra-
tion methods is stored within the spectrophoto-
meter separately for each cell type. The period of
validity of the zero adjustment for concentration
methods can be edited in the System settings.
When a zero adjustment has already been per-
formed for the inserted cell type and the se-
lected method, the date of the most recent zero
adjustment is displayed in the info line.
When a zero adjustment is required, following
screen will pop up:
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1. Insert the zero cell according to cell type.
Zero adjustment starts automatically and,
if the zero adjustment is passed, a tick 1
appears in the status display field for the
Zero adjustment.
In the case of a method which only mea-
sures the sample at a single wavelength
the absorbance of the Zero value 2is also
displayed.
2. With a cell inserted, the zero adjustment
can be repeated manually by tapping on the
Start zero button 3.
3. Tapping on the OK button accepts the zero
adjustment value for the method.
4. The screen changes to show the concentra-
tion measurement screen (see page 7).
5. The instrument is ready to start measuring
the sample.
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NOTE
The instrument provides a Quick Zero function.
With this the zero adjustment is performed for
all wavelengths which are used with all
Spectroquant®test kits. This function can be
activated via "System (Instrument settings)" –
"Quality".
4 Operation – 4.3 Zero Adjustment for preprogrammed methods
4.3.1 Notes on Zero Adjustment
Zero adjustment with round cells
•Only use clean, scratch-free round cells and
distilled water.
The minimum filling level is 20 mm. A ready
zero cell is contained within the scope of deliv-
ery of the spectrophotometer
•A ready zero cell can, in principle, be used for
an indefinite period of time. We recommend,
however, that you regularly check the zero cell
for visible contamination and scratches and
refill or exchange it if necessary (at least every
24 months)
•Insert the round cell until it touches the bot-
tom of the round cell compartment
Zero adjustment with rectangular cells
•With rectangular cells, zero adjustment must
be carried out using the same cell type (manu-
facturer and cell material [e.g. optical glass,
quartz glass, plastic]) as the one that will
be used for measurement. This is important
because cells of different manufacturers have
different absorption characteristics. When
changing the cell type, repeat the zero adjust-
ment with the new type
•Prior to zero adjustment, clean the rectangular
cell and fill it with distilled water. The minimum
filling level is 20 mm
•Rectangular cells always have to be inserted
in the cell compartment with the same orien-
tation for measurement and zero adjustment
(e.g. cell inscription always on the left side)
•Insert the rectangular cell until it touches
the bottom and left edge of the holder. The
opaque sides of the rectangular cell must point
to the front and rear
4.3.2 When to Repeat the Zero
Adjustment?
We recommend that you repeat the zero
adjustment in the following cases:
•If the spectrophotometer was subject to me-
chanical stress such as strong shock or trans-
port
•If the ambient temperature has changed by
more than 5 °C since the last zero adjustment
•At least once a week. The interval to repeat a
zero adjustment is set in the instrument to 7
days. You can change this under "System (In-
strument Settings)"
•If a new cell type (different manufacturer, dif-
ferent glass type) is used
•Basically, each time you want to measure with
the highest possible accuracy
NOTE
If an interval to repeat a zero adjustment is set
you will be prompted to repeat it after the inter-
val has passed. You can also repeat a zero ad-
justment by selecting a method, then touching
the "Settings" icon. Choose "Zero adjustment"
and insert a zero cell to start the measurement.

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4 Operation – 4.4 Measurements
4.4 Measurements
The spectrophotometer can be used to perform the measurements listed below.
Type of Measure-
ment
Description
Concentration •Preprogrammed methods that can be executed using Spectroquant®test
kits or self-prepared reagents
••User-programmed methods
Absorption /
transmission
•Single-wavelength measurements for establishing the absorbance or
transmission of solutions
•Multiple-wavelength measurements for establishing the absorbance or
transmission of solutions
Spectrum •Programmed methods for establishing the absorbance or transmission of
solutionsoveradenedwavelengthrange
Kinetics •Programmed methods for establishing the absorbance or transmission of
solutionsoveradenedperiod
Quality checks Instrument supported analytical quality assurance:
•Instrument check (AQA 1)
•Method-specicsystemcheck–preprogrammedforallSpectroquant®
standards (AQA 2)
•Pipette volume control (PipeCheck)
•Check interferences from foreign substances (MatrixCheck)
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