Sutter Instrument dPatch SutterPatch User manual

SERVICE & SUPPORT
Thank you for choosing Sutter Instrument products. To guarantee the
highest standards of quality and performance, every instrument is fabricated
on site by highly skilled technicians. The instrument contained herein has
been assembled with care and tested to assure it meets rigid quality control
standards.
We hope that our instruments and products continually meet your needs.
However, should a problem arise, please contact our technical support sta
to discuss your concerns. If the instrument requires factory service, we will
furnish shipping instructions. Items under warranty will be repaired free of
any costs for parts or service. Both delivery and return shipping costs are
the responsibility of the owner.
This product carries a limited warranty of 2 years for parts and labor.
Our oce hours are 8:00 am to 5:00 pm Pacific Standard Time, Monday
through Friday.
Phone [phone]
Fax [phone]
Intl. Phone [phone]
Intl. Fax [phone]
Sutter Instrument
One Digital Drive
Novato, CA 94949
[e-mail]
www.sutter.com
dPatch
®
DIGITAL PATCH CLAMP AMPLIFIER SYSTEM
with
SutterPatch
®
DATA ACQUISITION, MANAGEMENT AND ANALYSIS SOFTWARE
QUICK START GUIDE
rev. 2/20/20

Acquire Data
Membrane Test
DIGITAL PATCH CLAMP AMPLIFIER SYSTEMS OVERVIEW
Please read these instructions carefully before installation. This guide covers both
the dPatch
®
and dPatch-2 systems. If you have any questions or need additional
information, contact Sutter Instrument.
OPERATING SYSTEM REQUIREMENTS
Windows 10: 64-bit versions
macOS 10.11 (El Capitan) or later
INSTALL HARDWARE
1. Attach dPatch headstage(s) to amplifier front panel: HEADSTAGE 1 & 2. dPatch
headstages can be attached to any headstage port; they are not “matched” to
a port.
2. OPTIONAL: Attach dPatch Screw Terminal Board to amplifier rear panel
-> DIGITAL OUTPUTS, or dPatch Expansion Panel to DIGITAL OUTPUTS &
AUXILIARY I/O.
3. Attach USB cable to USB 3.0 port on amplifier rear panel and your computer.
The connector on the dPatch rear panel must click in place.
4. Attach power cord to amplifier rear panel and a grounded power outlet.
5. Power on the amplifier.
INSTALL IGOR PRO / SUTTERPATCH SOFTWARE
1. Power on computer.
2. If you have internet access, download the latest version of the SutterPatch
software installer from www.sutter.com/AMPLIFIERS/SutterPatch.html
3. If internet access is not available, attach the included USB flash drive to your
computer.
4. Double-click on ‘sutterpatch_win_full’ (Windows) or ‘sutterpatch_mac_full’
(macOS).
5. Follow the installer prompts for current Igor software.
6. Open Igor Pro and activate the Igor Pro license as instructed:
i. Serial Number:
ii. Activation Key:
TEST SYSTEM
1. Model Cell:
a. Attach Model Cell to HEADSTAGE 1 and tighten the collar.
b. Attach ground wire to HEADSTAGE 1 and Model Cell (gold plugs).
c. Surround with aluminum foil shielding and ground it to the headstage
ground connector.
2. Click icon to launch Igor:
3. SutterPatch welcome screen will open. Press ‘Start’.
4. On dashboard window:
I. Click on the Acquire Data icon:
II. Click on the Membrane Test icon:
5. Scope + Analysis windows open:
(Actual analysis values depend on dPatch filter settings)
I. With the Model Cell switch in Bath position, click the Bath button.
Pipette Resistance = ~10 MΩ
II. With the Model Cell switch in Seal position, click the Seal button.
Seal Resistance > 10,000 MΩ
III. With the Model Cell switch in Cell position, click the Cell button.
Access Resistance = ~10 MΩ
Membrane Resistance = ~500 MΩ
Membrane Capacitance = ~28 pF
Move the Model Cell and shielding to HEADSTAGE 2 (if installed), set the Scope
window to ‘Headstage 2’, and repeat steps I–III.
BACK PANEL
affix label here
DIGITAL PATCH CLAMP AMPLIFIER SYSTEM
QUICK START GUIDE dPATCH®/ dPATCH-2
SUTTER INSTRUMENT
19 1
37 20
PIN FUNCTION
1 - 16 Digital Output 1 - 16
17 - 19 +5 V
20 - 35 Ground
36 - 37 +5 V
DIGITAL OUTPUTS 1-16
DIGITAL OUTPUTS PINOUT