Thermo Scientific 1851 Operating instructions

Models:
1852, 1854, 1856 and 1857
Forma Laminar Airflow Workstation
Class 100
Operating and Maintenance Manual
Manual No: 7001850 Rev. 6

Model 1850 Series _________________________________________________________________________
i
Read This Instruction Manual.
Failure to read, understand and follow the instructions in
this manual may result in damage to the unit, injury to operat-
ing personnel, and poor equipment performance.
CAUTION! All internal adjustments and maintenance must
be performed by qualified service personnel.
The material in this manual is for information purposes only. The
contents and the product it describes are subject to change with-
out notice. Thermo Scientific makes no representations or war-
ranties with respect to this manual. In no event shall Thermo be
held liable for any damages, direct or incidental, arising out of or
related to the use of this manual.
MANUAL NO. 7001850
6 24102/HD-1508 9/6/07 Updated 1857 electrical schematic (225241 ballast to 500034) ccs
5 23871/SI-9779 4/3/07 Black outlet 460022 (low volume) replaced by white 460024, schematics ccs
4 21732/HD-1464 8/15/05 Updated parts list ccs
-- 21077/HD-1376 9/20/02 Removed certifiers list, added reference to website ccs
3 19029/HD-1295 6/19/00 Noted FLA rating with exclusion ccs
2 18826/HD-1286 2/25/00 Added mercury disposal warning ccs
-- 18582/HD-1269 11/4/99 Added airflow test sheet to Certification Test Procedures ccs
1 17913/HD-1237 12/7/98 Add info on levelers to prevent vibration-B. Morrison ccp
0 -- 9/87 Original manual
REV ECN DATE DESCRIPTION
WARNING! Lamps, thermometers and ther-
moregulators contain mercury. Do not put in
trash. Recycle or dispose as hazardous waste.

Model 1850 Series _________________________________________________________________________
ii
Alerts the user to important operating and/or maintenance instructions. May be used alone or with other safety sym-
bols. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform the instructions and procedures associated with
this symbol.
Hazard. Do not touch. Instructions associated with this symbol should only be carried out when using special hand-
ing equipment or when wearing special, protective clothing.
Potential biological hazards. Proper protective equipment and procedures must be used when following instructions
associated with this symbol. Reference O.S.H.A. Regulation 1910-1030.
Potentially hazardous energy. Equipment being maintained or serviced must be turned off and locked off to prevent
possible injury. Reference O.S.H.A. Regulation 1910-147.
Hot surface(s) present which may cause burns to unprotected skin or to materials which may be damaged by elevated
temperatures
Warning. Skin damage and/or eye injury can result from the light produced by ultra violet light sources installed in
this equipment. Never work in this unit with the ultra violet light operating.
* Always use the proper protective equipment (clothing, gloves, goggles etc.).
* Always dissipate extreme cold or heat, or wear protective clothing.
* Always follow good hygiene practices.
* Each individual is responsible for his/her own safety.

Model 1850 Series _________________________________________________________________________
iii
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Model 1850 Series _________________________________________________________________________
iv
Table of Contents
Section 1 - Receiving . . . . . . . . . . . . . . . . . . . . . . . . . . . .2 - 1
1.1 Preliminary Inspection . . . . . . . . . . . . . . . . . . . . . . .2 - 1
1.2 Visible Loss or Damage . . . . . . . . . . . . . . . . . . . . . .2 - 1
1.3 Responsibility for Shipping Damage . . . . . . . . . . . .2 - 1
Section 2 - Introduction . . . . . . . . . . . . . . . . . . . . . . . . .2 - 1
2.1 Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2 - 1
Section 3 - Installation and Start-Up . . . . . . . . . . . . . .3 - 1
3.1 Removal of Shipping Blocks . . . . . . . . . . . . . . . . . .3 - 1
3.2 Installing the Feet . . . . . . . . . . . . . . . . . . . . . . . . . . .3 - 1
3.3 Location and Leveling . . . . . . . . . . . . . . . . . . . . . . . .3 - 1
3.4 Optional Stand . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3 - 1
3.5 Power Connection . . . . . . . . . . . . . . . . . . . . . . . . . . .3 - 1
3.6 General Recommendations . . . . . . . . . . . . . . . . . . . .3 - 1
3.8 Start-Up Procedure . . . . . . . . . . . . . . . . . . . . . . . . . .3 - 1
3.9 Cabinet Shutdown . . . . . . . . . . . . . . . . . . . . . . . . . . .4 - 1
Section 4 - Operation . . . . . . . . . . . . . . . . . . . . . . . . . . .4 - 1
4.1 Controls and Indicating Devices . . . . . . . . . . . . . . . .4 - 1
Section 5 - Testing Procedures . . . . . . . . . . . . . . . . . . . .5 - 1
5.1 HEPA Filter Leak Test (DOP Test) . . . . . . . . . . . . . .5 - 1
5.2 Air Velocity Profile Test . . . . . . . . . . . . . . . . . . . . . .5 - 1
Section 6 - Routine Maintenance . . . . . . . . . . . . . . . . . .6 - 1
6.1 Checking the Static Pressure Gauge “Zero” . . . . . . .6 - 1
6.2 Replacing the Pre-Filters . . . . . . . . . . . . . . . . . . . . . .6 - 1
6.3 Replacing the Fluorescent Lamp . . . . . . . . . . . . . . . .6 - 1
Section 7 - Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7 - 1
7.1 Replacing the Supply HEPA Filter . . . . . . . . . . . . . .7 - 1
7.2 Replacing the Blower and/or Motor . . . . . . . . . . . . .7 - 1
7.3 Replacing the Blower Speed Control . . . . . . . . . . . .7 - 1
Section 8 - Troubleshooting . . . . . . . . . . . . . . . . . . . . . .8 - 1
8.1 Troubleshooting Guide . . . . . . . . . . . . . . . . . . . . . . .8 - 1
Section 9- Specifications . . . . . . . . . . . . . . . . . . . . . . . . . .9-1
Section 10 - Parts List . . . . . . . . . . . . . . . . . . . . . . . . . . .10-1
Section 11 - Electrical Schematics . . . . . . . . . . . . . . . . . .11-1
Section 12 - Warranty Information . . . . . . . . . . . . . . . .12-1

Model 1850 Series __________________________________________________________________Receiving / Introduction
2 - 1
Section 1 - Receiving
1.1 Preliminary Inspection
This item was thoroughly inspected and carefully packed
prior to shipment and all necessary precautions were taken to
ensure safe arrival of the merchandise at its destination.
Immediately upon receipt, before the unit is moved from the
receiving area, carefully examine the shipment for loss or dam-
age. Unpack the shipment and inspect both interior and exterior
for any in-transit damage.
1.2 Visible Loss or Damage
If any loss or damage is discovered, note any discrepancies
on the delivery receipt. Failure to adequately describe such evi-
dence of loss or damage may result in the carrier refusing to
honor a damage claim. Immediately call the delivering carrier
and request that their representative perform an inspection. Do
not discard any of the packing material or move the shipment
from the receiving area.
1.3 Responsibility for Shipping Damage
For products shipped F.O.B. Marietta, Ohio, the responsi-
bility of Thermo Fisher Scientific ends when the merchandise is
loaded onto the carrier’s vehicle.
On F.O.B. Destination shipments, Thermo and the carrier’s
responsibility ends when your Receiving Department personnel
sign a free and clear delivery receipt.
Whenever possible, Thermo will assist in settling claims
for loss or in-transit damage.
Section 2 - Introduction
2.1 Description
The horizontal laminar flow clean cabinet provides a par-
ticulate-free work environment for the performance of sterile
and/or dust sensitive procedures. The unit is recommended for
use in sterile apparatus assembly, media preparation sterility
testing, sterile filling and related industrial and biomedical pro-
cedure.
The cabinet provides product protection only. The opera-
tor/technician is exposed to any particulate, aerosol or gas
released from the work procedures. Therefore, procedures
involving viable agents, work involving drugs, or chemicals
that produce a toxic, allergic or carcinogenic response in
humans should not be performed within this unit.
Consult an institutional bio-safety officer or an industrial
hygienist before beginning any procedure that might endanger
the operator or contaminate the environment.

Section 3 - Installation and Start-Up
3.1 Removal of Shipping Blocks
Before putting the unit into operation, remove the card-
board shipping block from the front and back of the blower.
Remove the prefilter and the prefilter grille to gain access for
block removal.
3.2 Installing the Feet
The packing list includes eight white rubber “stick-on”
feet. Install these feet before placing the unit in its final loca-
tion.
3.3 Location and Leveling
Locate the cabinet on a level surface in an area of mini-
mum temperature change. The cabinet should be placed away
from personnel traffic, air-conditioning or heating ductwork,
and/or laboratory windows and doors. Proper cabinet location is
essential as drafts can disrupt critical airflow characteristics and
allow room contaminants to enter or escape the cabinet work
area.
Place the cabinet on an existing table or counter. The depth
of the table or table must be at least 36 inches to accommodate
the unit.
The cabinet weighs approximately 475 lbs.
Allow a 4” minimum clearance between the top of the cab-
inet and the laboratory ceiling to ensure an adequate air supply
to the blower.
Place a bubble-type level on the work surface and ensure
the cabinet is level.
3.4 Optional Stand
If the optional stand is to be used, adjust the leg levelers to
obtain the correct height (30”-36”) for the work surface. Make
sure all four levelers are in full contact with the floor.
3.5 Power Connection
Connect the power cord to a grounded dedicated power
source. Refer to the electrical data plate mounted on the unit,
or the electrical schematic, for exact electrical specifications.
3.6 General Recommendations
• Keep the activity in the room to a minimum when the cabi-
net is in use.
• Keep all laboratory doors closed to prevent drafts that will
disturb critical airflow characteristics.
• Segregate clean and dirty materials.
• Do not place anything on the intake grille, or block the pre-
filter grille.
• The operator should work at a normal pace, avoiding rapid
arm movements.
• Practice good aseptic technique to ensure safe use of the
cabinet.
• To increase the life of the HEPA filters, check the pre-fil-
ters regularly and replace them as necessary (Section 6.2).
3.7 Cabinet Wipe-down
Before operating the cabinet, allow the unit to run for
about 30 minutes. Then turn the blowers off and wipe the entire
interior surface with a laboratory detergent/disinfectant to
remove any remaining surface dirt.
3.8 Start-Up Procedure
1. Turn the cabinet lights on.
2. Check the intake grille to ensure that it is not blocked.
3. Turn on the blower on and allow the unit to warm up for
15 minutes before use.
4. Wash your hands and lower arms with germicidal deter-
gent.
5. Disinfect the entire work area.
6. Place everything needed at least 6 inches inside the
cabinet.
Model 1850 Series __________________________________________________________________Installation and Start-Up
3 - 1

3.9 Cabinet Shutdown
1. Surface decontaminate all surfaces with the appropriate
disinfectant and enclose all equipment that has been in
direct contact with the bedding.
2. Allow the cabinet to run for at least five minutes with no
activity to allow time for all airborne contaminants to be
purged from the work area.
3. Remove all equipment.
4. Wipe down all interior surfaces with the appropriate
disinfectant for the work being performed.
Section 4 - Operation
4.1 Controls and Indicating Devices
The High Efficiency Particulate Air (HEPA) filter is the
most critical component of the cabinet. These filters consist of
continuous sheets of fiberglass paper, pleated over rigid corru-
gated separators and mounted in a wooden frame. The filters
are extremely delicate and should never be touched or permit-
ted to get wet. HEPA filters are 99.99% efficient for particles
0.3 micron in diameter, but are not effective against chemicals
in their gaseous state.
Ambient air enters the cabinet through the pre-filter and is
pushed by the blower into the positive pressure plenum behind
the HEPA filter. Air pressure is evenly distributed in the plenum
and is pushed horizontally through the HEPA filter to the work-
zone where it provides a better than Class 100 clean environ-
ment. All workzone air exits the front of the cabinet, blowing
directly at the operator.
The cleanest part of the workzone is the area closest to the
back of the cabinet and nearest the HEPA filter. A ‘clean’ object
placed in the cabinet is subject to contamination of three types.
1. Down-stream contamination occurs when a ‘dirty’ object
is placed between the HEPA filter and a ‘clean’ object.
Contaminants from the ‘dirty’ object are picked up and
deposited on the sterile and/or particulate-free object.
2. Cross-stream contamination occurs when an object in
the cabinet is exposed to contaminants generated lateral-
ly to the ‘clean’ object. This type of contamination is
usually generated by rapid arm movements of the opera-
tor, personnel traffic, or room drafts. Lateral airflow pat-
terns in excess of 200 FPM can easily disturb the 90-110
FPM airflow velocity of the cabinet.
3. Backwash contamination occurs due to high velocity
turbulence generated down-stream from the object.
When laminar flow is accessible to both sides of the
object, the area of turbulence down-stream extends
approximately three times the width of the object, or six
times the width of the object if the laminar flow is
accessible to only one side of the object.
Model 1850 Series _____________________________________________________________________________Operation
4 - 1

Blower Switch
The blower switch controls the power to the blower motor.
Light Switch
The light switch controls power to the fluorescent lamps in
the work area or the optional ultra-violet lamp.
Static Pressure Gauge (In. W.G.)
The optional static pressure gauge measures the air pres-
sure differential across the filters, providing an indication of fil-
ter “loading”. As the filters become dirty, the resistance to air
passage increases, and the reading on the static pressure gauge
increases accordingly. When the reading increases by 50%,
check the cabinet airflow with a thermoanemometer. The filters
should be replaced if they are shown to be inefficient due to
loading.
Note: Static pressure gauge should not be used as a direct
measure of airflow.
Blower Speed Control
Note: The blower speed is set at the factory and should only be
changed by a qualified technician.
The blower speed control is located beside the blower
switch on the control panel. The speed control is used to adjust
the air velocity from the blower motor. A clockwise turn of the
adjustment screw increases the air velocity.
Reset Button
The Reset button is an in-line circuit breaker. If an over-
load condition occurs, the circuit breaker will trip. The Reset
button will protrude from the panel. Depressing the button will
reset the circuit breaker.
Electrical Outlet (Accessory)
There is a single 115V electrical outlet located on the right
end of the control panel (as viewed from the front).
Ultra-Violet Light (Optional)
Potential eye damage may result from viewing
the light produced by ultra violet light sources
installed in this equipment. Never work in this
unit with the ultra violet light operating.
Cabinets may be equipped with an ultra-violet germicidal
light as optional equipment. These lamps lose their effective-
ness over a period of time and should be replaced about every 6
months. A single 115V (1A maximum) electrical outlet for con-
necting the optional ultra-violet light is located beside the Reset
button.
5 - 1
Section 5 - Testing Procedures
A list of certification companies is included on the Thermo
website, or call the Technical Services department. See Page iii.
Note: As shipment stress can affect the integrity of the cabinet,
certification by specialized service personnel is necessary after
installation.
Certification is recommended:
~ Annually
~ If cabinet is moved
~ After replacing HEPA filter
~ After any inside cabinet service work is done
5.1 HEPA Filter Leak Test (DOP Test)
1. Place the DOP aerosol generator so that DOP is intro-
duced into the openings under the pre-filter.
2. Turn on a photometer and calibrate in accordance with
the manufacturer’s instructions.
3. Scan the downstream sides and the perimeter of each
HEPA filter by passing the photometer probe in slightly
overlapping strokes over the entire filter surface, with
the nozzle of the probe not more than one inch from the
surface. Scan the entire periphery of the filter plus the
junction between the filter and the filter mounting frame.
Scanning should be done at a traverse rate of not more
than two inches per second or ten feet per minute.
Note: DOP penetration must not exceed 0.01% as measured by
a linear photometer.
5.2 Air Velocity Profile Test
A drop in line voltage will cause a corresponding
drop in airflow. Check the voltage to the unit
before airflow measurement. Support the power
systemas needed.
Airflow measurements should be taken by using a ther-
moanometer mounted on a support stand. The measurements
should be taken following a lateral pattern six inches inside the
front opening and six inches from the solid cabinet surfaces
with six inch centers. These readings should fall in the range of
70-110 LFPM (linear feet per minute) with an average of about
90 LFPM.
If the airflow readings do not average about 90 LFPM,
adjust the blower speed control to attain this value. As the fil-
ters load with particulate, airflow will begin to drop. If adjust-
ment of the speed control cannot compensate for the filter load-
ing (the control is turned up to full and at least 80 LFPM can-
not be obtained), then the filters must be changed.
Model 1850 Series _______________________________________________________________________Testing Procedures

Thermo Fisher Scientific

Thermo Fisher Scientific

Thermo Fisher Scientific