Agilent Technologies 1260 Infinity User manual

Agilent Technologies
Agilent 1260 Infinity
Thermostatted Column
Compartment
User Manual

Agilent 1260 Infinity TCC User Manual
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Manual Part Number
G1316-90014
Edition
05/2013
Printed in Germany
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Agilent 1260 Infinity TCC User Manual 3
In This Guide…
In This Guide…
This manual covers the Agilent 1260 Infinity Thermostatted Column
Compartments (G1316A TCC).
1 Introduction to the Column Compartment
This chapter gives an introduction to the TCC, instrument overview and
internal connectors.
2 Site Requirements and Specifications
This chapter provides information about site requirements and
specifications for the module.
3 Installing the Column Compartment
This chapter describes the installation of the thermostatted column
compartment.
4 Using the Module
This chapter explains the operational parameters of the module.
5 How to optimize the Column Compartment
This chapter provides information on how to optimize the thermostatted
column compartement.
6 Troubleshooting and Diagnostics
Overview about the troubleshooting and diagnostic features.
7 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.

4 Agilent 1260 Infinity TCC User Manual
In This Guide…
8TestFunctions
This chapter describes the TCC’s built in test functions.
9 Maintenance
This chapter describes the maintenance and repair of the TCC.
10 Parts for Maintenance
This chapter provides information on parts for maintenance.
11 Identifying Cables
This chapter provides information on cables used with the 1260 Infinity
series of HPLC modules.
12 Hardware Information
This chapter describes the detector in more detail on hardware and
electronics.
13 Appendix
This chapter provides addition information on safety, legal and web.

Agilent 1260 Infinity TCC User Manual 5
Contents
Contents
1 Introduction to the Column Compartment 9
Introduction 10
System Overview 13
Column-Identification System 16
Column Switching Valve (Optional) 18
2 Site Requirements and Specifications 21
Site Requirements 22
Physical Specifications 25
Performance Specifications 26
3 Installing the Column Compartment 27
Unpacking the Column Compartment 28
Optimizing the Stack Configuration 29
Installation Information on Leak and Waste Handling 34
Installing the Column Compartment 38
Flow Connections of the Column Compartment 41
Placing Columns 45
4 Using the Module 47
Leak and Waste Handling 48
Solvent Information 49
5 How to optimize the Column Compartment 51
Optimizing the Performance of your Column Compartment 52
6 Troubleshooting and Diagnostics 53
Overview of the Module’s Indicators and Test Functions 54
Status Indicators 56
Available Tests depending on User Interfaces 58
Agilent Lab Advisor Software 59

6 Agilent 1260 Infinity TCC User Manual
Contents
7 Error Information 61
What Are Error Messages 62
General Error Messages 63
TCC Error Messages 69
8TestFunctions 77
Thermostat Function Test 78
Pressure Test 81
Column Thermostat Temperature Calibration 82
9 Maintenance 91
Warnings and Cautions 92
Introduction to Maintenance 94
Overview of Maintenance 95
Cleaning the Module 96
Changing Column Identification Tags 97
Replacing Head Parts of Column Switching Valve 99
Correcting Leaks 102
Replacing the Module’s Firmware 103
10 Parts for Maintenance 105
Column Switching Valve 2 Position/6 Port 106
Kits 107
Plastic Parts 108
Leak Parts 109
11 Identifying Cables 111
Cable Overview 112
Analog Cables 114
Remote Cables 116
BCD Cables 119
CAN/LAN Cables 121
External Contact Cable 122
Agilent Module to PC 123
Agilent 1200 Module to Printer 124

Agilent 1260 Infinity TCC User Manual 7
Contents
12 Hardware Information 125
Firmware Description 126
Electrical Connections 129
Interfaces 131
Setting the 8-bit Configuration Switch (without On-Board LAN) 138
Instrument Layout 142
13 Appendix 143
General Safety Information 144
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002/96/EC) 147
Lithium Batteries Information 148
Radio Interference 149
Sound Emission 150
Use of Solvents 151
Agilent Technologies on Internet 152

8 Agilent 1260 Infinity TCC User Manual
Contents

9
Agilent 1260 Infinity TCC User Manual
Agilent Technologies
1
Introduction to the Column
Compartment
Introduction 10
System Overview 13
Leak and Waste Handling 13
Column-Identification System 16
Column Switching Valve (Optional) 18
This chapter gives an introduction to the TCC, instrument overview and internal
connectors.

10 Agilent 1260 Infinity TCC User Manual
1Introduction to the Column Compartment
Introduction
Introduction
Main Features
The Agilent 1260 Infinity Thermostatted Column Compartment is a
stackable temperature- controlled column compartment for LC. It is used
for heating and cooling to meet extreme requirements of retention time
reproducibility.
The main features are:
•Peltier heating and cooling from 10 degrees below ambient up to 80 °C
with high heating and cooling speeds for maximum application
flexibility and stability.
•Holds up to three 30 cm columns and optimized design gives minimum
dead volumes and maximum efficiency.
•Two independently programmable heat exchangers contribute volumes
of only 3 µL and 6 µL.
•Electronic column- identification module as standard for GLP
documentation of column type and major column parameters.
•Optional high- quality Rheodyne®column switching valve with ceramic
stator- face assemblies for prolonged lifetime.
For specifications, see “Performance Specifications” on page 26.

Agilent 1260 Infinity TCC User Manual 11
Introduction to the Column Compartment 1
Introduction
System Overview
The Concept of Heating and Cooling
The design of this thermostatted column compartment uses column
heating and cooling devices with Peltier elements. The solvent entering the
column compartment is heated up or cooled down to a settable
temperature with two low- volume heat exchangers (3 µl on left side, 6 µl
on right side), made of a short piece of capillary 0.17 mm i.d. leading
through a heat exchanger. The heat exchanger is designed such that it can
function simultaneously as an air heater. The shape of the heat exchanger
surface allows the area around the column to be kept at a similar
temperature level as the liquid running through the column. This is done
by thermal convection and radiation between the heat exchanger fins. This
design ensures that the column and the solvent flowing through it are
almost at the same temperature.
Actual temperature control is accomplished at the heat exchanger. The
solvent cools down or heats up on its transfer from the heating block to
the column inlet. This depends on several factors: flow rate, setpoint
temperature, ambient temperature and column dimensions.
In a flow- through temperature regulation system, there are necessarily
slightly different temperatures at different positions. If, for example, the
temperature set by the user is 40 °C, then the heat exchanger is regulated
to a temperature of 40.8 °C which is different by a certain offset (here
0.8 °C). The solvent temperature at the column entry would be about
39 °C.
The actual temperature displayed on the user interface is always the
derived temperature taken at the heat exchanger, corrected by the offset
explained above.
Any type of heated column compartment brings one important
consequence for column temperature equilibration. Before an equilibrium
is reached, the whole mass of column, column packing, and solvent volume
inside the column has to be brought to the selected temperature. This
depends on several factors: flow rate, setpoint temperature, ambient
temperature and column dimensions. The higher the flow rate, the faster
the column equilibrates (due to thermostatted mobile phase).

12 Agilent 1260 Infinity TCC User Manual
1Introduction to the Column Compartment
Introduction
Figure 1 on page 12 shows a setpoint temperature of 40 °C. Some time
after entering the setpoint the heat exchanger has reached its temperature
and the control activity starts. The TEMPERATURE NOT READY signal will be
cancelled 20 seconds after the sensed temperature was within a range of
± 0.5 °C of the setpoint (other values can be set via the user interface).
However this does not necessarily mean that the column has already
reached the correct temperature. The equilibration of the column may take
longer. Stability of the pressure signal is a good indication for equilibrium.
Figure 1 Equilibration of Heat Exchanger and Column Temperature
The temperature calibration and verification is described in the Service
Manual.
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Agilent 1260 Infinity TCC User Manual 13
Introduction to the Column Compartment 1
System Overview
System Overview
Leak and Waste Handling
The 1200 Infinity Series has been designed for safe leak and waste
handling. It is important that all security concepts are understood and
instructions are carefully followed.

14 Agilent 1260 Infinity TCC User Manual
1Introduction to the Column Compartment
System Overview
Figure 2 Leak and waste handling concept (overview - typical stack configuration as an
example)
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Agilent 1260 Infinity TCC User Manual 15
Introduction to the Column Compartment 1
System Overview
The solvent cabinet (1) is designed to store a maximum volume of 6 L
solvent. The maximum volume for an individual bottle stored in the
solvent cabinet should not exceed 2.5 L. For details, see the usage
guideline for the Agilent 1200 Infinity Series Solvent Cabinets (a printed
copy of the guideline has been shipped with the solvent cabinet, electronic
copies are available on the Internet).
The leak pan (2) (individually designed in each module) guides solvents to
the front of the module. The concept covers also leakages on internal
parts (e.g. the detector’s flow cell). The leak sensor in the leak pan stops
the running system as soon as the leak detection level is reached.
The leak pan's outlet port (3, A) guides excessive overfill from one module
to the next, as the solvent flows into the next module’s leak funnel (3, B)
and the connected corrugated waste tube (3, C). The corrugated waste
tube guides the solvent to the next lower positioned module’s leak tray
and sensor.
The waste tube of the sampler’s needle wash port (4) guides solvents to
waste.
The condense drain outlet of the autosampler cooler (5) guides condensate
to waste.
The waste tube of the purge valve (6) guides solvents to waste.
The waste tube connected to the leak pan outlet on each of the bottom
instruments (7) guides the solvent to a suitable waste container.

16 Agilent 1260 Infinity TCC User Manual
1Introduction to the Column Compartment
Column-Identification System
Column-Identification System
The Agilent 1260 Infinity Thermostatted Column Compartment is equipped
with a column- identification system. It allows to read and write
column- specific information to and from an optionally available
column- identification tag (part of the HPLC starter- kits available for 1220,
1260 and 1290 Infinity pumps or seperately as P/N 5062- 8588).
Figure 3 Column-Identification System
Table 1 on page 17 shows the information that can be stored. The
information fields can be edited via the user interface.
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Agilent 1260 Infinity TCC User Manual 17
Introduction to the Column Compartment 1
Column-Identification System
The number of injections will be updated each run to create a column
lifecycle (history). The user interface allows to edit all information.
Table 1 Column-Identification Module Information
Item Example Comment
Product number 79916OD-552
Serial number 950522 Date of manufacturing
Batch number 1675
Geometry 100 mm × 2.1 mm
Stationary phase ODS Hypersil
Particle size 10 µm
Number of injections 1267 See below.
Maximum pressure allowed 400 bar
Maximum temperature recommended 70 °C
Maximum pH recommended 12
Column void volume
NOTE
If a column switching valve (see “Column Switching Valve (Optional)” on page 18) is
installed in the module, the update of the number of injections depends on the position of
the column switching valve. For example, if the left column is selected, the right column is
not updated, and vice versa. If no column switching valve is installed both sides are
updated at the same time.

18 Agilent 1260 Infinity TCC User Manual
1Introduction to the Column Compartment
Column Switching Valve (Optional)
Column Switching Valve (Optional)
Figure 4 Location of Column Switching Valve

Agilent 1260 Infinity TCC User Manual 19
Introduction to the Column Compartment 1
Column Switching Valve (Optional)
Two Column Selection
The valve can select either column 1 or column 2. The offline column is
sealed by connecting head to tail. Switching should be done when the flow
is off and the pressure is zero.
Figure 5 Column 1 Active
Figure 6 Column 2 Active
NOTE
Before switching the valve, switch off the pump or set the flow to zero. Keeping the flow on
while the valve is switched can cause exceeding the maximum pressure. This will stop
method or sequence execution.
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20 Agilent 1260 Infinity TCC User Manual
1Introduction to the Column Compartment
Column Switching Valve (Optional)
Precolumn Back-flushing
The sample is injected into series- connected precolumn and analytical
column. After the valve has switched, the analytical column flow continues
in normal direction. Only the precolumn is back- flushed, eluting highly
retained peaks directly to the detector.
Figure 7 Precolumn Back-flushing
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