Alpha MED Scientific MED64 MED-C11 User manual

Product Manual
MED Multi-well Connector
P/N: MED-C11
Themostsensitivemicroelectrodearraysystem
forinvitroextracellularelectrophysiology

Information in this document is subject to change without
notice.No part of this document may be reproduced or
transmitted without the expressed written permission of Alpha
MED Scientific Inc.
While every precaution has been taken in the preparation of this
document, the publisher and the authors assume no
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have been caused directly or indirectly by this document.
© 2016 Alpha MED Scientific Inc. All rights reserved.
Version: 1.00; April 1, 2016
Alpha MED Scientific Inc.
Saito Bio-Incubator 209, 7-7-15, Saito-asagi,
Ibaraki, Osaka 567-0085, Japan
E-mail: [email protected]
Website: http://www.med64.com

MED64 Product Manual - MED Multi-well Connector (MED-C11) i
Contents
1. MED Multi-well Connector Components and Functions 1
1-1. Components and their functions..............................................................1
2. Installation 3
2-1. Connecting the MED Multi-well Connector to the MED64 Head Amplifier........3
2-2. Running the MED64-Allegro System and checking the noise ........................4
3. Instruction for use 5
3-1. Installing the MED Multi-well Probe on to the MED Multi-well Connector........5
3-2. Electrode orientation in the MED Multi-well Connector ................................6
MED 4 Well Probe [MED-P5NF30] ............................................................6
MED 8 Well Probe [MED-P5N801 / MED-P5N811].......................................7
3-3. Running the MED64-Allegro System.........................................................8
3-4. Stimulation with the MED64-Allegro System..............................................9
4. Troubleshoot 10
5. Maintenance 11
5-1. Cleaning of the contact pins..................................................................11
5-2. Sterilization........................................................................................12
5-4. Storage..............................................................................................12
6. Cautions 12
7. Terminal assignment 13
8. Warranty 13
9. Specifications 14

Contents
ii MED64 Product Manual - MED Multi-well Connector (MED-C11)

Product Manual - MED Multi-well Connector (MED-C11) 1
1. MED Multi-well Connector Components and Functions
1. MED Multi-well Connector Components and Functions
The MED Multi-well Connector (MED-C11) is the Connector for the MED64-Allegro System. It connects
the MED Multi-well Probe to the MED64 Head Amplifier (MED-A64HE1S) allowing recording extracellular
signals from 4 (or 8) cultured samples in a MED Multi-well Probe.
Figure 1. System diagram for the MED64-Allegro System.
1-1. Components and their functions
MED Multi-well Connector unit
Figure 2. MED Multi-well Connector. Front view (left) and back view (right).
(1) Ground terminal
Connect to the GND terminal on the MED64 Head Amplifier with the green grounding wire.
(2) Screw hole
Use these hole to install the Connector onto the MED64 ThermoBase.
(3) Probe port
Install the MED Multi-well Probe here.
(4) Output terminal
Connect to the INPUT 1-16CH, 17-32CH, 33-48CH, 49-64CH on the MED64 Head Amplifier with the
MED Connector Cable (20 pins).
MED Multi-well MED Multi-well MED64 Head Amp. MED64 Main Amp. PC and Mobius
Stimulator Digitizer
Probe Connector
(1) (2) (3) (4)

2 Product Manual - MED Multi-well Connector (MED-C11)
1. MED Multi-well Connector Components and Functions
Accessories
Figure 3. Accessories for the MED Multi-well Connector.
(1) MED Connector Cable (4 sets)
Connects the Output terminals to the INPUT terminals on the MED64 Head Amplifier (MED-A64HE1S).
(2) Grounding wire
Connects the Grounding terminal to the SIGNAL GND of the MED64 Head Amplifier (MED-A64HE1S)
for grounding.
(3) Test board
Install into the Probe port. Use it to check the MED64 Allegro System is working appropriately.
(1) MED Connector Cable (20 pins) -4 sets-
(2) Grounding wire (3) Test-board

Product Manual - MED Multi-well Connector (MED-C11) 3
2. Installation
2. Installation
2-1. Connecting the MED Multi-well Connector to the MED64 Head
Amplifier
• Read product manuals for all other components of the MED64-Allegro System prior to installation.
1. Place the MED Multi-well Connector on a stable table and AWAY from any equipment which generates
an electric or magnetic filed (e.g. power supply unit, peristaltic pump, heater, refrigerator, etc.).
2. Connects the 4 output terminals of the MED Multi-well Connector tothe INPUT terminals of the MED64
Head Amplifier with the MED Connector Cables. Make sure that the terminals with same numbers
are connected. (e.g. 1-16CH to INPUT 1-16CH).
3. Place all cables AWAY from any equipment that generates and electric or magnetic filed.
• The MED Connector Cable is very sensitive to noise and vibration. Make sure that the cable
stays away from any equipment that generates electric or magnetic filed, and sits on a stable
table where it cannot be disturbed (i.e. bumped into or displaced during recording).
4. Connects the Ground terminal to the SIGNAL GND in the MED64 Head Amplifier (MED-A64HE1S) with
the green grounding wire to properly ground the Connector.
CAUTION:
Make sure to ground the MED Multi-well Connector to the Head Amplifier. Otherwise considerable exog-
enous noise could be introduced.
Figure 4. Connecting the MED Connector cables (20 pins) to the MED Multi-well Connector.
Figure 5. Connection of the MED Multi-well Connector to the MED64 Head Amplifier (left) and Ground terminal on the
MED Multi-well Connector connected with the grounding wire (right).
1-16ch 17-32ch 33-48ch 49-64ch

4 Product Manual - MED Multi-well Connector (MED-C11)
2. Installation
2-2. Running the MED64-Allegro System and checking the noise
Install the Test board to the MED Multi-well Connector and check the noise. The baseline noise should
be around several microvolts at all 64 channels as seen in the Figure 6. If noise is larger than a few
microvolts, check the followings:
1. All equipment are grounded correctly.
2. Amplifiers, MED Multi-well Connector, and all Connector cables are placed AWAY from any equipment
which generates electric or magnetic field. (e.g. power supply unit, peristaltic pump, heater). Pay
particular attention for the amplifiers’ power supply unit.
3. All cables are firmly connected.
Figure 6. Appropriate noise level for the MED64-Allegro System

Product Manual - MED Multi-well Connector (MED-C11) 5
3. Instruction for use
3. Instruction for use
3-1. Installing the MED Multi-well Probe onto the MED Multi-well
Connector
1. Clean the MED Multi-well Probe with kimwipe soaked with 70% ethanol before sliding into the
Connector.
• The leads in the MED Multi-well Probe contact with gold pins of the MED Multi-well Connector.
(Refer to Figure 15 in the page 11) Salt residue, or even finger print can cause the contact
pins to rust or accumulate residue build up.
2. Slide in the MED Multi-well Probe (the side with the terminals) gently and horizontally to the Probe
port. Firmly press to make a secure connection.
CAUTION:
• Spilling liquid onto the pins will cause rust and adversely affect conduction. Be extremely careful
not to get the pins wet, especially with saline solution.
Figure 7. MED Multi-well Probe (MED-5N811) with its terminals on the top (left) and installing the MED Multi-well
Probe onto the MED Multi-well Connector (right).
Figure 8. MED Multi-welll Connector where Probe is installed.

6 Product Manual - MED Multi-well Connector (MED-C11)
3. Instruction for use
3-2. Electrode orientation in the MED Multi-well Connector
The Figure 9 and 10 shows the electrodes’s orientation when the MED Multi-well Connector is installed
with its terminal oriented towards the right side. Each well in the MED Multi-well Probe has 8 (or 16)
reference electrodes as well as 16 (or 8) recording electrodes, those are connected independently to
the terminal.
MED 4well Probe [MED-P5NF30]
Figure 9. Electrode orientation for the MED-P5NF30 (4 well) in the MED Multi-iwel Connector and terminal
assignment.

Product Manual - MED Multi-well Connector (MED-C11) 7
3. Instruction for use
MED 8well Probe [MED-P5N801 / MED-P5N811]
Figure 10. Electrode orientation for the MED-P5N801/811 (8 well) in the MED Multi-well Connector and terminal
assignment.

8 Product Manual - MED Multi-well Connector (MED-C11)
3. Instruction for use
3-3. Running the MED64-Allegro System
Signals acquired through the MED Multi-well Connector is displayed in the Mobius as following:
4 Well Probe 8 Well Probe
Modules [Display 4x4 Channels], [Display 2x4 Channels] are also available to display signals acquired
through the MED Multi-well Connector.
Figure 11. Displaying signals for each well with the [Display 4x4 Channels].
Figure 12. Displaying signals for each well with the [Display 2x4 channels].
Channel#Well# Channel#Well#
Ch1‐81Ch33‐40 5
Ch9‐16 2 Ch41‐48 6
Ch17‐24 3 Ch49‐56 7
Ch25‐32 4 Ch57‐64 8
Channel#Well# Channel#Well#
Ch1‐16 1 Ch33‐49 3
Ch17‐32 2 Ch50‐64 4

Product Manual - MED Multi-well Connector (MED-C11) 9
3. Instruction for use
3-4. Stimulation with the MED64-Allegro System
The MED64-Allegro System allows you to stimulate each well sequentially. Use Mobius Acquire MED64R2
Data w/Stim’s Step1-8 to stimulate one (or 2 channels) per well (sample) sequentially. Inactivate the
Step 5-8 when the 4 Well Probe is used.
Set the Trace interval (stimulus interval) for longer than 5 seconds since the amplifiers need at
least 5 seconds to recover after stimulation.
Figure 13. An example for stimulation to the 4 Well Probe. Stimulation is applied to Well#1-4 with interval of 10
seconds.
Stim Stim Stim Stim Stim

10 Product Manual - MED Multi-well Connector (MED-C11)
4. Troubleshoot
4. Troubleshoot
When noise appears during your experiments, it is important to identify the source of the noise. Likely
reasons are:
• Damaged electrodes of the MED Probes
• Uncleaned and/or damaged contact pins in the Connector.
• Loosely connected the cables.
• Improper grounding.
• Equipment that generates and electric or magnetitic field (e.g. power supply, peristaltic pump,
heater).
• Environment
Poor contacts caused by damaged or uncleaned contact pins can cause problems such as noises or/and
faulty recording. Identify this problem by using the Test board following this procedure:
Figure 14. The Test board installed in the MED Multi-well Connector.
Trouble shooting flow chart

Product Manual - MED Multi-well Connector (MED-C11) 11
5. Maintenance
5. Maintenance
5-1. Cleaning of the contact pins
The 64 leads of a MED Multi-well Probe connect to the Connector via small contact pins. Clean the gold
contact pins with following procedure if you observe noise from dirty pins.
Figure 15. MED Multi-well Probe. View from side (left) and its structural scheme (right).
1. Loosen the 4 screws at the bottom of MED Multi-well Connector to remove the upper “Connector unit
from the bottom “Base unit.
Figure 16. MED Multi-well Connector, bottom view (left) and reassembled Connector (right).
2. Clean the contact pins of the printed circuit board with a kimwipe soaked in 70% ethanol. Clean the
64 pins one by one vertically. Do NOT clean the pins laterally, as it may damage the contact pins.
(See Figure 17.) Do not touch the contact pins with bare hands.
Figure 17. Cleaning the contact pins. Clean them vertically.
3. Reunite the Base unit and Connector unit.
Connector
unit
Base unit

12 Product Manual - MED Multi-well Connector (MED-C11)
6. Cautions
5-2. Sterilization
Wipe the Multi-well Connector with a lint-free cloth soaked in 70% ethanol, and allow to dry.
CAUTION:
• Do NOT autoclave as it may damage the MED Multi-well Connector.
5-3. Storage
Store in a cool dry area. Avoid exposure to high temperatures or humidity.
6. Cautions
• DO NOT spill medium or any other liquid on the contact pins.
• DO NOT give strong mechanical shock by putting heavy material on the unit or dropping the unit.
• DO NOT touch the contact pins on the connector unit with bare hands or fingertips when they are
cleaned. This may cause rust and adversely affect conduction.
What should I do if medium is spilled onto the contact pins?
Stop your experiment immediately. Disassemble your Connector and clean the contact
pins with kim-wipe soaked in 70% ethanol, and allow to dry.

Product Manual - MED Multi-well Connector (MED-C11) 13
7. Terminal assignment
7. Terminal assignment
Figure 18 shows terminal assignment for the MED Multi-well Connector.
Figure 18. Terminal assignment for the MED Multi-well Connector.
8. Warranty
This product will be repaired with new or refurbished parts, free of charge, for one (1) year from the
date of original purchase in the event of a defect in materials or workmanship.
The product warranty covers failures due to defects in materials or workmanship which occur during
normal use. It does NOT cover damage incurred during shipment or problems which are caused by
products not supplied by Alpha MED Scientific. In addition, this warranty does not cover problems
resulting from alteration, accident, misuse, neglect, faulty installation, maladjustment of user controls,
improper maintenance, modifications or service by anyone other than AMS or damage attributable to
acts of God.
Ref: Reference electrode
NC: No assignment

14 Product Manual - MED Multi-well Connector (MED-C11)
9. Specifications
9. Specifications
Connector unit
MED Connector Cable
Specifications may not be satisfied depending upon the type of computer or operating environments
used. Only for use in animal studies research. Specifications and external appearance are subject to
change without notice. ThermoClampTM-1 is a trade mark for AutoMate Scientific, Inc.
MED Probe securing mechanism Slide in
Out put 20-pin MDR system connector x 4
Material Aluminum (Gold for contact pins)
Weight 700g
Dimensions 210 x 83 x 34 (mm)
Plug 20-pin MDR
Plug cover Metal
Length 2 m

MED-C11

April 1, 2016
Alpha MED Scientific Inc.
Saito Bio-Incubator 209, 7-7-15, Saito-asagi,
Ibaraki, Osaka 567-0085, Japan
Phone: [phone] FAX:[phone]
http://www.med64.com [email protected]
Manufactured by Alpha MED Scientific Inc.
©2016 Alpha MED Scientific Inc.