Analytik Jena UVP Hybridizer User manual

81-0169-01 Rev W
UVP Hybridizer, UVP HybriLinker and UVP
Multidizer Hybridization Ovens
Operating Instructions
UVP Hybridizer UVP Multidizer UVP HybriLinker
IMPORTANT: To familiarize yourself with the correct operation procedures, read these instructions
before operating your UVP Hybridizer, UVP Multidizer or UVP HybriLinker system.
_________________________________________________________________________
Analytik Jena US
2066 W. 11th Street, Upland, CA 91786
Tel: [phone]
Fax: [phone]
Web Site: www.us.analytik.jena.com
Ultra-Violet Products Ltd.
Unit 1, Trinity Hall Farm Estate
Nuffield Road, Cambridge CB4
1TG UK
Tel: [phone] Fax:
[phone]

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Table of Contents
Table of Contents ........................................................................................................ 2
Introduction ................................................................................................................. 3
Hybridization Oven Specifications ............................................................................. 4
Setup Instructions ....................................................................................................... 5
Operating Instructions ................................................................................................ 7
Care and Cleaning .....................................................................................................12
Replacement Parts ....................................................................................................13
Accessories.................................................................................................................14
Maintenance, Repair and Technical Assistance......................................................16
Hybridization Techniques.........................................................................................17

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Introduction
The UVP Hybridizer is a cost-effective, high-performance, 10-bottle hybridization oven with state-of-the-art
microprocessor temperature and variable speed controls. The UVP HybriLinker™System is designed with a combination of
hybridizer and crosslinker components. This equipment is intended to be used in the laboratory environment.
The UVP Multidizer™System is designed as a multifunction unit with a variety of motion options. The lower chamber
operates in the same manner as the Hybridizer. However, the Multidizer has an additional upper chamber for the use of a
reciprocating shaker tray, an orbital rocker tray, or an acrylic carousel. Refer to the “Accessories” section of this manual for
ordering information.
The Hybridizer can be set to operate anywhere from 10 to 15 RPM, enabling consistent saturation of samples for washing
or hybridizing. Having the ability to remove the rotary carousel wheel and replace it with an interchangeable rocker plate
enables all hybridization protocols in one reliable benchtop unit. An easily removable protective tray under the carousel
allows easy clean-up of spilled media.
A touch-sensitive keypad and microprocessor provide accurate temperature control from ambient 10°C to 99.9°C. The
keypad is located just below a large LED readout. Once the desired hybridization temperature is entered, the readout
displays the current temperature inside the chamber. The chamber environment is calibrated at 68.0°C. At this
temperature, the accuracy is +/-0.5°C. An internal circulating fan assists in uniformly distributing heating throughout the
chamber.
Two different hybridizer bottle sizes are available for use within the hybridizer using the standard carousel provided with
the unit. The rotary carousel wheel can hold up to twenty (20) 35 x 150 mm bottles or ten (10) 35 x 300 mm bottles. In
addition, combinations of both size bottles may be used at the same time. An optional carousel kit for oversized bottles is
available.
There may be build up of pressure within the hybridization bottles when they are taken from ambient to hybridization
temperature. To help in relieving some of this pressure, preheat the solutions and bottles. Also, be sure bottles are opened
at the same temperature at which the hybridization was done. Do not allow the bottles to cool before opening.
To ensure the hybridization bottles remain leak proof and pressure proof, prevent temperatures above 70°C. If
temperatures above this are used without first relieving the pressure within the bottle, there is a risk that bottles will leak
and/or break due to internal pressure build-up.
If the bottles are accidentally taken above 70°C without relieving the pressure, DO NOT open the hybridizer door. Turn off
the hybridizer and allow it to cool before opening the door and checking the contents of the oven. Should the bottles be
used above 70°C, relieve the pressure by unscrewing and re-tightening the cap.
If using radioactive material, see the “Decontamination” section of this manual.
The HybriLinker System and the Multidizer System are designed to be carried or lifted by two people.

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Hybridization Oven Specifications
UVP Hybridizer Hybridization Oven
Part Numbers Volts/Hz
95-0030-01 115V/60Hz
95-0030-02 230V/50Hz
95-0030-03 100V/50Hz
Specifications
Net Weight: 43 lbs
Temperature: Ambient +10°C to 99.9°C
Temperature Stability: Inside Oven: +/-0.3°C to 68°C
Inside Bottles: +/-0.1°C to 68°C
Temperature Uniformity: Inside Oven: +/-0.1°C to 68°C
Inside Bottles: +/-0.1°C to 68°C
Temperature Display: LCD
Heating Element: 1250 watts
Rotation Speed: Off, 10 to 15 RPM
Bottle Capacity: Twenty (20) 35 x 150 mm, Ten (10) 35 x 300 mm
Dimensions: Exterior 17.5W x 16.125H x 15D in. (445 x 410 x 381mm)
Interior 14W x 10.75H x 10.75D in. (356 x 273 x 273mm)
UVP HybriLinker System
The HybriLinker System combines a hybridization oven and a crosslinker in one space-saving unit. Each section
has separate power supplies for independent operation.
Part Numbers Volts/Hz
95-0031-01 115V/60Hz
95-0031-02 230V/50Hz
95-0031-03 100V/50Hz
Specifications
Unit Net Weight: 60 lbs
Dimensions: Exterior 17.5W x 24H x 15D in. (445 x 610 x 381mm)
Hybridization Oven
Temperature: Ambient +10°C to 99.9°C
Temperature Stability: Inside Oven: +/-0.3°C to 68°C
Inside Bottles: +/-0.1°C to 68°C
Temperature Uniformity: Inside Oven: +/-0.1°C to 68°C
Inside Bottles: +/-0.1°C to 68°C
Temperature Display: LCD
Heating Element: 1250 watts
Rotation Speed: Off, 10 to 15 RPM
Bottle Capacity: Twenty (20) 35 x 150 mm, Ten (10) 35 x 300 mm
Interior Dimensions: 14"W x 10.75"H x 10.75D (356 x 273 x 273mm)
Crosslinker
Microprocessor controls 8 watt Shortwave UV (254nm) tubes
Large LED displaying time or energy Tactile membrane keypad
Preset or user-selected UV time exposure settings Internal interlocking safety system
Preset or user-selected UV energy settings

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Maximum UV energy setting: 999,900 microjoules per cm2
Interior Dimensions: 11.375W x 3.75H x 11.5D in. (288 x 95 x 292 mm)
UVP Multidizer Hybridization Oven
Part Numbers Volts/Hz
95-0340-01 115V/60Hz
95-0340-02 230V/50Hz
95-0340-03 100V/50Hz
Specifications
Net Weight: 75 lbs (34 kg)
Temperature Display: LCD
Dimensions: Exterior 17.5W x 28.5H x 15D in. (445 x 724 x 381 mm)
Upper Chamber
Temperature: Ambient +10°C to 80°C
Temperature Stability: Inside Oven: +/-0.3°C to 68°C
Inside Bottles: +/-0.1°C to 68°C
Temperature Uniformity: Inside Oven: +/-0.1°C to 68°C
Inside Bottles: +/-0.1°C to 68°C
Heating Element: 500 Watts
Shaker Tray Speed: 54 - 106 cycles/min.
Carousel Rotation: 12 - 20 rpm
Lower Chamber
Same as the UVP Hybridizer
Setup Instructions
Two different bottle sizes are available for use within the hybridizer. The rotary wheel carousel can hold up to twenty (20)
35 x 150 mm bottles or ten (10) 35 x 300 mm bottles. In addition, combinations of both bottle sizes may be used at the
same time.
To insert bottle holder carousel (Fig. 1), slide the two wheels onto the rod and tighten the thumb screws (Fig. 2).
Insert the rod into the holder on the right wall of the hybridizer chamber. Then, slide the rod into the holder on
the left wall of the chamber.
To adjust for various bottle lengths, loosen the thumb screws on the bottle clip wheel and adjust to desired
width.
To insert bottles into bottle clips, gently push the bottle into the clips (Fig. 3). Insert one side at a time for large
bottles.
If offset/skewed bottle positioning is desired (Fig. 4), loosen the thumb screw for only one bottle clip wheel.
Slightly rotate the wheel until the required angle is obtained. Retighten the thumb screw. (Note: Offset or
skewed bottle positioning can allow
for better mixture of bottle
contents.)
NOTE: ALWAYS USE AN EVEN NUMBER OF BOTTLES AND LOAD BOTTLES OPPOSITE EACH OTHER TO BALANCE THE
BOTTLE HOLDER. THIS PREVENTS EXCESSIVE WEAR OF THE BEARINGS AND DRIVE MOTOR.

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WheelS
Rod
Thumb
Screw
Fig. 1 Inserting Bottle Holder
Carousel
Fig. 2 Adjusting for
Bottle Lengths
Fig. 3 Inserting Bottles
Fig. 4 Offset/Skewed Bottle
Positioning
Fig. 2 Adjusting for bottle
lengths

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Installing the Rollers and Optional Trays
To insert rollers in the hybridization oven carousel, slide the rollers into the
receptacle on the left side of the chamber first, followed by the right side.
To install the Reciprocating Shaker Tray (standard with the Multidizer), place
the tray over the rollers in the hybridization oven.
To install the Rocker Tray (optional on all units), remove the metal carousel from the chamber. Place the tray into the
chamber with the coupling arm to the right side. Place the coupling sleeve over the motor drive shaft such that the pins on
the inside of the coupling sleeve engage the slots in the motor drive shaft of the chamber. Rotate the sleeve counter-
clockwise 30° until locked in place. To remove the tray, reverse the process.
To install the Orbital Motion Tray (optional on all units), open the chamber door, remove the carousel, connect the power
cable to the orbital tray and to the power port inside the unit, and place the tray in the oven so the switch is accessible.
Turn the hybridization oven Motor and Power switches ON, and turn the orbital tray Power switch ON.
Operating Instructions
Please note that this equipment is not intended for interconnection with any other devices. Use of this equipment other
than intended may create a safety hazard and/or malfunction.
Using the Hybridization Oven
1. Place the unit on a level working surface and provide adequate room in front of the door to open it easily.
2. Plug the female end of the power cord into the unit.
3. To turn on the main power supply, depress the Power switch toward “ON” on the right side of the control
panel.
Reciprocating
Shaker Tray
Rocker
Tray
Orbital Motion Tray

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4. To adjust the temperature setting to the necessary degree, press either the “UP” or “DOWN” buttons below
the temperature control display to raise or lower the displayed degree (displayed in degrees Celsius). This
setting is adjustable from ambient 10°C to 99.9°C. See “Setting the Temperature Controls” for more detail.
5. To turn on the motor for the bottle holder, press the motor control power switch to the “ON” position.
6. To adjust the bottle carousel to the desired RPM, simply turn the motor control knob clockwise to increase
the RPM and counterclockwise to decrease the RPM.
Minimum RPM = OFF/10 Maximum RPM = 18
NOTE: The inside of the hybridizer should be cleaned with a damp cloth. The bottle carousel assembly can be
cleaned with soap and water.
Setting the Temperature Controls
Whenever power is applied to the controller, the software revision number is displayed for several seconds. While the
software revision number is being displayed, the intensity of the display digits alternates between full and half brightness,
and on some models the two outside decimals points blink on and off. After several seconds, the display reverts to
showing the controlled temperature. In this mode, a single decimal point is illuminated (at most) and the display intensity
is steady.
Altering the Setpoint
The current setpoint value can be altered using the “UP” and “DOWN” buttons while the setpoint is being
displayed. To change the setpoint from normal mode, proceed as follows:
7. Momentarily press then release either the “UP” or the “DOWN” button. The LED display will flash to
indicate that the displayed value is the current setpoint.
8. Increase or decrease the setpoint value by pressing the “UP” or “DOWN” buttons respectively. If either
button is held down for more than several seconds, the setpoint value will increase or decrease
continuously.
9. When the desired setpoint is reached, wait approximately five seconds without pressing either button.
The display will then revert to normal mode showing the actual temperature.
The new setpoint becomes effective and is stored in the non-volatile memory when the display reverts to the
normal mode.
Calibrating the Temperature Reading
The hybridizer is calibrated at the Analytik Jena factory. AJ recommends temperature recalibration be performed
at the AJ factory as recalibration by the user may void the warranty. Obtain an RGA (Returned Goods
Authorization) number from AJ prior to returning a unit to the factory. Call AJ’s customer service department in
the USA in Upland, California at [phone] or in the UK in Cambridge, UK at [phone].
Decontamination
IMPORTANT: These decontamination methods may not remove all contaminants. Refer to Federal, State, and
Local Guidelines and Biological Protocols to assure decontamination.
Bottles and Caps
Soak items in a diluted detergent solution overnight.
Remove from detergent and rinse items with distilled water.
If items are still contaminated, gently scrub with an abrasive cloth or brush. If necessary, continue to
soak items in the detergent solution for a longer period of time.

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Oven Chamber
The oven chamber and drip pans may be decontaminated by wiping clean with a decontaminating agent, then
repeat with distilled water.
NOTE: If any unit requires service, a Returned Goods Authorization (RGA) number must be obtained from AJ’s
customer service department prior to returning any item to AJ. If radioactive or biological hazardous material has
been present within the unit, radioactive decontamination and biological cleanup must be performed BEFORE
returning the unit, as per current federal, state, and local guidelines and biological protocols.
Using the Crosslinker
The crosslinker portion of the HybriLinker operates independently from the hybridizer. The crosslinker provides an
exposure instrument which utilizes shortwave 254nm ultraviolet energy for many applications and protocols. The
crosslinker’s internal microprocessor measures and controls UV output, ensuring maximum energy efficiency. UV
crosslinking takes seconds as compared to hours for oven baking. The UV crosslinking procedure optimizes the use of the
ultraviolet energy while ensuring that crosslinking is consistent.
WARNING! The crosslinker is a powerful source of ultraviolet (UV) radiation. Even though it is not easily accessible, do not
attempt to disengage or override the internal safety interlocks. Exposure to UV radiation may result. If the UV sources
remain on when the door is open, the unit is malfunctioning and use should be discontinued until the unit has been
serviced. Do not expose unprotected eyes or skin to UV radiation.
NOTE: This equipment is not intended for interconnection with any other devices. Use of this equipment other than as
intended may create a safety hazard and/or malfunction.
1. Place the unit on a level working surface. Be sure there is
adequate room in front of the door to open it easily.
2. Plug the female end of the power cord into the unit.
3. Plug the male end of the power cord into a properly grounded
electrical outlet. The proper operating voltage of the Crosslinker is
found on the product information label. Note: For 230V models, or those requiring special power cord
connectors, ensure that the proper configuration of the male connector or the plug has been properly connected
to the power cord.
4. Turn the On/Off switch to the On position. NOTE: WHEN TURNED ON THE CROSSLINKER DEFAULTS TO THE
LAST USED UV EXPOSURE SETTING.
5. The last UV exposure setting will now be displayed on the LCD and the last function setting will be noted by
glowing red spot(s) on the display panel.
6. Place your sample requiring exposure into the chamber.
7. The Crosslinker can be operated on the following settings:
Preset Ultraviolet Energy Exposure
1. Press “PRESET” on the tactile touchpad. Then press “ENERGY”. The red light at each position should now be
lit and the preprogrammed UV exposure setting of 120,000 microjoules per cm² is displayed in the LED as
1200. Note: The LCD displays “1200”. This is in hundreds and therefore must be multiplied by 100 to obtain
an exposure.
2. Press “START”. After a slight delay to energize the UV tubes, the LCD will begin to count down. The unit
automatically stops at the end of the exposure cycle and will beep five times. Exposure is now complete.
NOTE: Though the preset UV exposure setting is factory set at 120,000 microjoules per cm², it is possible to
change this if necessary, as follows:
a. Press and hold “PRESET”on the tactile touchpad until you hear an audible signal. Then press
“ENERGY”on the touchpad.

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b. Set your new preset UV exposure by pressing the numbers on the touchpad.
Remember: The number indicated on the LCD display must be multiplied by 100 to determine the
proper exposure setting. The new setting will then appear on the display.
c. Press “ENTER” on the touchpad. The new setting is now installed.
Preset Ultraviolet Time Exposure
1. Press “PRESET” and then “TIME” on the tactile touchpad. The red light at each position should now be lit and
the preprogrammed UV exposure time of 2 minutes should be displayed on the LCD.
2. Press “START” on the touchpad. After a slight delay to energize the UV tubes, the LCD will begin to count
down. NOTE: THE TIME EXPOSURE IS SET IN MINUTES AND TENTHS OF A MINUTE.
3. The unit will automatically stop at the end of the exposure cycle and will beep five times. Exposure is now
complete.
NOTE: Though the PRESET ULTRAVIOLET TIME EXPOSURE is factory set to 2 minutes, it is possible to
change this if necessary, as follows:
a. Press and hold “PRESET” on the touchpad until you hear an audible signal. Then press “TIME”.
b. Set your new preset UV time exposure by pressing the numbers on the tactile touchpad. The new
setting will appear on the LED display.
c. Press “ENTER,” and the new setting will be installed.
User-Set UV Energy Exposure
Sometimes it may be necessary for you to set your own standards for exposure. This is easily accomplished as
follows:
1. Press “ENERGY”on the tactile touchpad, then set your energy exposure requirements by pressing the
numbers corresponding to your desired UV exposure time on the touchpad. The energy exposure
settings should now be displayed as flashing on the LCD.
NOTE: Your energy exposure settings displayed must be multiplied by 100. If the settings are correct,
press “ENTER”on the touchpad.
2. Press “START”on the touchpad. After a slight delay to energize the UV tubes, the LCD will begin to
count down. The unit will stop automatically at the end of the exposure cycle and will beep five times.
Exposure is now complete.
User-Set UV Time Exposure
Setting your own UV time exposure can be achieved as follows:
1. Press “TIME”on the tactile touchpad and then set your requirements by pressing the corresponding
numbers. Your time exposure settings will be displayed flashing on the LCD. Remember that your time
exposure settings are displayed in minutes and tenths of a minute. If your settings are correct, press
“ENTER”on the tactile touchpad.
2. Press “START”. After a slight delay to energize the UV tubes, the LCD will begin to count down. The unit
will stop automatically at the end of the exposure cycle and will beep five times. Exposure is now
complete.
3. At the end of the exposure cycle, open the door and remove the sample.
Operational Notes:
To abort an exposure, press “STOP” on the tactile touchpad. The LCD will display the remaining
exposure time.
To restart an aborted exposure, press “START”on the touchpad. The exposure will continue from the
point at which the exposure was aborted.
To reset an aborted exposure, press “RESET”. The LCD and touchpad will return to the last-used setting.
The unit will not operate with the door open. Opening the door during a cycle aborts the cycle.
Reclosing the door will reset the cycle to the last-entered cycle used. To restart, press the “START”key.