ARJAY ENGINEERING FLUOROCHECK II User manual

MODEL: FLUOROCHECK II
PPM OIL IN WATER MONITOR
USER MANUAL (REV: 3.0)
ARJAY ENGINEERING LTD.
2851 Brighton Road
Oakville (Toronto), Canada
L6H 6C9
Tel: ++1 [phone]
Fax: ++1 [phone]
North America: 1-800-387-9487
Web: www.arjayeng.com
Email: [e-mail]

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TABLE OF CONTENTS
THIS PAGE INTENTIONALLY LEFT BLANK TABLE OF CONTENTS ................................................. 2
SPECIFICATIONS .................................................................................................................................... 4
1.0 USE HAZARD INFORMATION ....................................................................................................... 5
2.0 INSTRUMENT OVERVIEW ............................................................................................................. 6
2.1 FEATURES ..................................................................................................................................................... 6
2.2 DESCRIPTION ................................................................................................................................................ 6
2.3 UNPACKING ................................................................................................................................................... 6
2.4 IMPORTANT INFORMATION ......................................................................................................................... 6
2.5 INSTRUMENT POWER UP ............................................................................................................................ 7
3.0 FLUOROMETRY PRINCIPLES AND METHOD OVERVIEW ........................................................ 8
3.1 FLUORESCENCE MEASUREMENT .............................................................................................................. 8
3.1.1 DIRECT WATER SAMPLE OVERVIEW .......................................................................................... 8
3.1.2 SOLVENT EXTRACTED SAMPLE OVERVIEW.............................................................................. 8
3.2 INSTRUMENT OPERATION OVERVIEW ...................................................................................................... 8
4.0 INSTRUMENT FUNCTION TUTORIAL ........................................................................................ 10
KEYPAD REVIEW ......................................................................................................................................... 10
POWER UP AND MAIN DISPLAY ................................................................................................................. 10
COMMUNICATION ....................................................................................................................................... 13
5.0 INITIAL SITE OR OIL SPECIFIC CALIBRATION ........................................................................ 15
5.1 SOLVENT EXTRACTION TECHNIQUE ....................................................................................................... 15
5.2 LED INTENSITY SET-UP .............................................................................................................................. 16
5.3 SITE CALIBRATION...................................................................................................................................... 16
5.4 CALIBRATING THE INSTRUMENT .............................................................................................................. 17
5.5 SETTING THE REFERENCE CUVET ........................................................................................................... 19
6.0 TESTING A SAMPLE ................................................................................................................... 21
6.1 CHOOSE YOUR TEST MODE AND CALIBRATION LOCATION ................................................................. 21
6.2 IMPORTANT MEASUREMENT NOTES ....................................................................................................... 21
6.3 SOLVENT EXTRACTION TEST ................................................................................................................... 21
6.4 SAMPLE SCREEN TEST .............................................................................................................................. 22
7.0 TROUBLESHOOTING .................................................................................................................. 23
7.1 SYMPTOMS .................................................................................................................................................. 23
7.2 MAINTENANCE, ERROR AND OTHER MESSAGES .................................................................................. 23
8.0 CUSTOMER SERVICE INFORMATION ....................................................................................... 24
APPENDIX A: SAMPLE PREPARATION METHOD ............................................................................. 25

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SPECIFICATIONS
Specifications are subject to change without notice:
Specification Details
Power Requirement: 12VDC, 0.5A
Power Supply Adapter: Input: 100-240VAC, 47-63Hz, 0.5A
Output: 12VDC, 1.5A
User Interface:
Display Four line x 16 character Backlit LCD with simultaneous display in PPM, Cal
Location, Method of Extraction
Input Interface Membrane Keypad
Communication Interface: RS-232, 9-Pin Female Serial connector, 9600 baud, 8 data bits, 1 stop bit, even
Instrument Performance:
Measuring Range 0 - 100 ppm (Higher ranges with sample dilution)
Instrument Accuracy ± 0.1 PPM
Note: The accuracy is based on oil type, calibration mode and operating
consistency
Calibration Memory A library of up to 10 calibrations can be stored
Calibration Curve Up to five concentration entry points per calibration to maximize accuracy
Environmental:
Operating Condition Bench Top; Portable
Operating Temperature Indoor Use: 15-40°C
Relative humidity ≤ 80% for 15 – 31 °C
Decreasing linearly to 50% for 31 – 40 °C
Altitude ≤ 2000 m
Installation Category II
Pollution Degree 2
Light Source:
Type UV LED; Excitation Wavelength: 360-370 nm
Expected LED Life 1000 Operating hours (FCII has an adjustable auto shutoff feature)
Excitation Filter Excitation wavelength: 354 ± 5 nm
Emission Filter Emission Wavelength: 450 nm ± 40 nm
Mechanical Specification:
Dimensions 280mm x 168mm x 110mm High
Weight 1 kg (2.4 lbs)
Approval Standards: EN 61010-1:2010, EN 61326-1:2013, IEC 61010-1:2010 (3rd Edition),
IEC 61326-1:2012
Power Supply Safety
Specification:
cULus, CE
NOTE:
The above specification and protection impairment may be affected if the equipment is not used as
specified by the manufacturer.

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1.0 USE HAZARD INFORMATION
CAUTION Indicates a potentially hazardous situation that may result in minor or
moderate injury.
WARNING Indicates a potentially or imminently hazardous situation which, if not
avoided, could result in death or serious injury.
DANGER Indicates a potentially or imminently hazardous situation which, if not
avoided, will result in death or serious injury.
NOTICE Indicates a situation which, if not avoided, may cause damage to the
instrument. Information that requires special emphasis.

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2.0 INSTRUMENT OVERVIEW
2.1 FEATURES
• Fluorescence technology
• Non-contact sensor
• Bench Top monitoring
• Instantaneous readings
• Multi-point automatic or manual calibration
• Auto Zero Function
• Temperature compensation
• LCD display in PPM or NTU or FLR value
2.2 DESCRIPTION
The FluoroCheck II is a bench top Oil in Water Monitor with a fixed excitation bandpass filter (354 nm)
and an emission bandpass filter (450 nm). It is designed specifically for the quantification of low ppm
concentration measurements of hydrocarbons (oil) in water. Through a calibration against a lab
analysis, the targeted aromatics in the oil are correlated to give a total oil reading.
The FluoroCheck II has many applications of use and will provide varying degrees of accuracy
depending on its set up.
• A single solvent-free sample in a cuvet can provide field screening of a direct water sample
using the Arjay supplied calibration. The reading accuracy can be improved by entering a site
calibration.
• A single solvent extracted sample can provide good readings using the Arjay calibration library
of crude oil or refined oil. (Refined oil calibration uses diesel)
• A single solvent extracted sample can provide a high degree of accuracy using a site specific
calibration correlated to a laboratory result.
• Following the procedures outlined in this manual and using procedures as indicated by the EPA
or ISO for sample preparation can provide highly accurate measurements specific to your
application.
Determine your own degree of accuracy required and follow procedures consistently for best results.
2.3 UNPACKING
Unwrap all packages carefully and compare contents with the packing list, making sure all items
arrived. If any part is missing, contact your local sales office. Inspect all components for damage that
may have occurred while the unit was in transit. If any parts appear damaged, contact the carrier
immediately. Be sure to keep all packing material for damage claims or for repacking should it become
necessary to return the unit.
2.4 IMPORTANT INFORMATION
• Sample and solvent disposal must comply with all applicable regulations.
• Always unplug the instrument before opening the panel or cleaning the instrument.

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• Use and store the instrument away from direct sunlight and away from areas where the
instrument may become wet.
• Wipe the cuvet exterior before placing it into the well. Take care not to spill any liquid into the
well.
• Reliable results depend on measurement accuracy and consistency.
• If this equipment is used in a manner not specified by the manufacturer, the protection provided
by the equipment may be impaired.
• Only accessories and parts approved or supplied by Arjay Engineering may be used for
operating, maintaining, and servicing this product.
• Use gloves and eye protection when handling hazardous liquids.
CAUTION Avoid direct contact with a UL LED that is powered on. Always turn the
power switch off before servicing or maintaining this instrument.
2.5 INSTRUMENT POWER UP
The FluoroCheck operates on 12 VDC power. A 100-240 VDC 47-63 Hz transformer is provided with
the unit. The 12 VDC jack of the power supply plugs into the receptacle on the back of the unit marked
power input. Plug the AC end to a suitable grounded power outlet.
The power switch is beside the power in receptacle and is marked (I) for on, (O) for Off.
See section 3.0 for detailed Instrument Function Description.

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3.0 FLUOROMETRY PRINCIPLES AND METHOD OVERVIEW
3.1 FLUORESCENCE MEASUREMENT
The fluorescence principle is based on the science that certain compounds, when subjected to a
specific wavelength of light energy (excitation) and can re-emit this light energy at one, or more, higher
wavelengths (emission). These emission wavelengths are registered as peaks.
Aromatic hydrocarbons, when subjected to a specific wavelength, will fluoresce an emission at a
predictable wavelength. This is considered it's signature wavelength. When the sample is in the cuvet
well of the instrument, it is is exposed to UV light energy (360-370 nm) from a high precision filtered
LED light source. This light energy excites the hydrocarbon molecules in the sample, which then emit
energy at peaks of approximately 450 nm. An emission filter in front of the photodetector allows only
fluorescence at 450 nm +/- 40nm to register, minimizing background interference caused by other
wavelengths. Through the instrument calibration, the measured fluorescence provides a direct
indication of the hydrocarbon concentration in the engineered units of your choice.
The ratio of aromatics to total oil is registered through a customer site calibration or Arjay factory
calibration to provide a display of total oil.
Since different oil types may yield different fluorescent intensities at similar concentrations, an
instrument calibration can be made specific to your application.
3.1.1 DIRECT WATER SAMPLE OVERVIEW
A direct water sample can be tested in the FluoroCheck. This is ideal for fast screening of aromatics in
a water sample without any sample conditioning. The Factory Calibration for refined or crude oils
eliminates the need for a site calibration. The results are more qualitative than quantitative but are
effective for trending and comparison of multiple tests. Samples are tested in the supplied 12mm
diameter (2 MIL) disposable cuvets.
3.1.2 SOLVENT EXTRACTED SAMPLE OVERVIEW
A solvent extraction procedure using Hexane or Pentane as indicated by EPA 1664 Rev 1 and ISO
9377-2 provides high accuracy results. The hydrocarbons are extracted from the water using solvent.
This clarifies and defines the sample to be tested. Background influences are left behind in the water
sample. The FluoroCheck can be calibrated against your local laboratory methods for correlated
results. The supplied disposable 4mm diameter (200 uL) cuvets are used for crude oil testing and the
12mm diameter (2 MIL) disposable cuvets are used for refined oil testing.
3.2 INSTRUMENT OPERATION OVERVIEW
The cuvets provided are disposable to avoid cross-contamination between samples. These are low
wavelength, high transmission disposable borosilicate cuvets. Use only Arjay supplied or
recommended cuvets. Inappropriate cuvets may not have the light transmission suitable for this
instrument or may not resist solvents.
Although the unit is shipped with factory preset calibrations to allow out-of-the-box use, for best
accuracy, the unit should be calibrated against a sample that has been tested by your local lab. This
will correlate the unit to your process water conditions and to your local lab methodology.
The FluoroCheck can store up to 10 different calibrations:
• Location 0 (DIESEL1) is factory calibrated for solvent extraction testing of refined oils.
• Location 1 (CRUDE1) is factory calibrated for solvent extraction testing of crude oils.

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• Location 2 (DIESEL2) is factory calibrated for direct water samples of refined oils.
• Location 3 (CRUDE2) is factory calibrated for direct water samples of crude oils.
• Locations 4 to 9 are available for customer site or oil specific calibrations.
After a calibration is stored, you do not need to re-calibrate the instrument if powering the instrument
on and off. However, a ‘ZERO’ should be performed daily or after power-up to compensate the
instrument for normal UV LED and electronic drift.
Calibration Reference Cuvet: A Reference Cuvet has been supplied with this instrument. A site
specific concentration value will be assigned to the Reference Cuvet that will represent your calibration
and oil type. This will allow frequent re-calibrations and checks without having to use prepared or lab
tested samples. This value will be assigned after your initial instrument calibration.
Crude Oil: REF A
Refined Oils (e.g. Diesel): REF B
Periodic re-calibrations using actual prepared or analyzed samples may be desired to maintain a good
correlation against local laboratory results.

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4.0 INSTRUMENT FUNCTION TUTORIAL
NOTICE This section offers a functional overview of the unit. It is recommended to have
the unit and a power source available to you while you review this section
KEYPAD REVIEW
The keypad is used to select the initial setup options, to zero and calibrate the instrument, and to
initiate routine testing. The following keys are available for use.
Numeric and alpha-Keys <0> to <9> Use the numeric keypad to enter a calibration standard value or
to choose menu options.
The <DISPLAY> key exits any menu to return to the normal operating display. While entering numeric
values in the menus, the <DISPLAY> key will reverse an entry to allow a correction.
The <CAL> key enters the menu to select the calibration from the stored calibration locations list or
perform a new calibration.
The <SETUP> key accesses the user-selected functions including diagnostics, units and mode.
The <ZERO> key compensates the normal UV LED and electronic drift.
The < . > key represents a decimal point for numeric entries.
The <ENTER> key registers numeric values or advances to the next screen.
POWER UP AND MAIN DISPLAY
Plug in the power supply to an AC source. Plug in the DC jack into the back of the Fluorocheck II and
turn the power switch to on (I). If already powered on, turn the switch off then on again to start this
tutorial.
A screen will momentarily flash with the Hardware and Software version. A countdown will begin to
allow the LED to warm up and stabilize.
A r j a y E n g i n e e r i n g
F l u o r o c h e c k I I
R e v : x x . x x x x x x
S / N : x x x x x x x
RESTARTING LED
War ming up....21
When complete, the display will read the Testing Mode that has most recently been used. It will be one
of these two.
S o l v e n t E x t r a c t i o n
1 4 . 6 m g / l
D i e s e l 1
O
R
Sample Screening
0 0 . 0 p p m
Cal 4
Samples can be tested in 2 different modes.

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1. Solvent Extraction: This is the most accurate test. It requires a Hexane or Pentane extraction of
the water sample following the extraction procedures indicated by EPA 1664 Rev 1 or ISO-9377-2.
The extraction procedure removes the hydrocarbons from the water into the solvent. This defines the
oil to be tested, clarifies the sample for better readings, and concentrates the extracted oil into the
solvent to provide a more intense response.
2. Sample Screening: This is for general water sample screening. A water sample can be inserted
into the instrument to determine if a fluorescence response is present. No response will confirm that
there are no aromatic hydrocarbons in the water. A response will indicate the likelihood of
hydrocarbons. The value indicated should be used for trending and process conditions and not for
precise quantitative purposes.
Press <SETUP>, to continue this tutorial.
A password is requested when entering any menu where a calibration or operation parameter can be
changed. The password is 2000. Key in <2000> now. If you enter a wrong number in this or any other
screen, you can press the Display key to backspace the curser. With 2000 now keyed in, press
<ENTER> to continue.
The SETTINGS screen offers a menu of user selectable operation settings and diagnostics
SETTINGS
1-Units 2-Mode
3-Auto Off 4-Diags
Press <1> for Units. This concentration readings can be displayed in different engineered units:
SETTINGS
1-ppm 2-mg/L 3-FLR
Current units:ppm
You can select the units of measurement you prefer to display. FLR refers to Raw Fluorescence units
and would be used for specialty applications where absolute values are not applicable. FLR is a millivolt
reading of the sensor.
The unit does not mathematically convert from one type of unit to another after calibration. Choose
your desired display mode prior to calibration.
Select the desired units now to continue this tutorial.
The SETTINGS menu will return.

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Press <4> for Diagnostics:
Temp.: 25C DIESEL2
FLR: 91.7mV
LED Power: 10.0mA
1-ChangemA E=Return
Temp.: 25 C. The FluoroCheck includes an onboard temperature compensation circuit to improve
results if ambient temperatures are different from the time of calibration. The present internal
temperature is displayed here.
DIESEL2. This indicates the present Calibration Storage Location that has been selected for the
instrument. (Note: Other storage locations may be shown on display).
FLR: 91.7 mV. This is the active Fluorescence that the sensor is presently reading. There is always
some offset Fluorescence, even with a clean sample. It is Zeroed out during calibration. Open the
sample lid now and you will see this reading change due to the background light entering the well.
Since this reading is always active, this screen is a helpful tool if you want to check and compare
samples for a fluorescence response.
LED Power: 10 mA. The LED setting can be adjusted to maximize its intensity for optimal accuracy
during a calibration. The LED intensity can be changed in this screen to determine an optimal
fluorescence value.You will be requested to enter a mA value into a Storage Location during calibration
With the FluoroCheck lid closed, observe the FLR reading on the screen. Now Press <1> to change
the LED intensity to a higher or lower value by 5 or more. The settable range is 0-25.5 mA. Enter
your new value and press <ENTER>. You will notice the FLR value has changed up or down
accordingly.
This technique will be used during the calibration procedure to optimize your calibration.
Press <ENTER> to return to the SETTINGS menu
Press <3> for Auto Off: This feature allows the user to select a preset time in which the LED will
automcatically shut off from no activity. This feature will extend the life of the LED. Enter the time
value in seconds (min. 60 sec.; max. 43,200 sec.) and press <ENTER>. When in shut off mode the
screen will show:
STANDBY MODE
Press any key to
restart unit...
Press <2> for Mode: This will allow you to choose the mode of sample delivery for your sample
testing. This screen can be accessed at any time if you wish to change to a different test mode.
Select Mode
1 Solvent Extraction
2 Sample Screening

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These are the two screens that can be used during routine testing
S o l v e n t E x t r a c t i o n
0 0 . 0 p p m
Cal 6
O
R
Sample Screening
0 0 . 0 p p m
Cal 6
The screen will depend on the test mode that you use. In most applications, you will routinely use the
same mode. The mode you last used will appear on power up.
After selecting the Mode type press <DISPLAY> and the operating screen will show Sample Screening
or Solvent Extraction screen depending on your selection.
The sensing circuit is constantly active and will be able to read any time a sample is inserted. Software
acknowledges a sample test change and initiates the test accordingly.
The Calibration Storage Location being used is indicated in the bottom left corner. When doing tests,
routinely check that you are you using the correct Calibration Location for your test.
A routine Zero of the instrument is recommended before each day of operation or when you change
test modes. The Zero compensates for any offset drift that may occur as components age.
To ZERO, you will need to insert a cuvet for the Test Mode you have chosen. For the Sample
Screening method, you will fill the appropriate cuvet to about 3/4 full with clean water. For the Solvent
Extraction, you will fill the appropriate cuvet to about 3/4 full with the pure solvent. This procedure is
not required to be done for this tutorial session.
Press the <CAL> key. Enter the password 2000 and press <ENTER>. The menu will allow you to
choose a calibration from the stored calibration locations list or perform a new calibration.
CALIBRATION
1-Select from list
2-Perform new cal
Current: xxxxxx
Select from list will allow you to choose a calibration that has been stored by the factory or by the
user. The factory calibrations are based on factory sourced oils. Actual oils at your site may indicate a
different response. The factory calibrations should only be used for qualitative indication and not
absolute quantitative readings. This procedure will be detailed during Calibration.
Perform new cal will allow you to perform a calibration using your own sample and then store it into a
Calibration location. This procedure will be detailed during Calibration.
The current Calibration Location being used is shown on the bottom line.
COMMUNICATION
Operations can be monitored from the RS232 port if a computer or printer is connected to the unit. This
may be desirable if logging or printed records are required.

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The RS232 serial port of FluoroCheck II is a DB9-pin female connector. Connect the serial port of
FluoroCheck II to computer serial port. The FluoroCheck II requires these settings in the device
receiving the data:
Baud rate 9600
Data bits 8
Stop bit 1
Start bit 1
Parity Even
In the computer, under the standard windows setup, the Hyper Terminal program resides in the
Communications file under Accessories. Click on the Hyper Terminal icon to open the program.
Cancel “Connection Description”, then open the file named Fluorocheck2 Hyper Terminal Emulator”
The following data output is sent by pressing the <0> key anytime while in a normal operating screen.
Cal Location
LED mA current
Display Value
This unit does not internally log data and does not provide time or date stamping.
This completes the Operation Tutorial.
The following section details how to calibrate and run a sample.

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5.0 INITIAL SITE OR OIL SPECIFIC CALIBRATION
Although the unit is shipped with factory preset calibrations to allow out-of-the-box use, for best
accuracy, the unit should be calibrated against a site sample that has been tested by your local lab.
This will correlate the unit to your process water conditions and to your local lab methodology.
The FluoroCheck can store up to 10 different calibrations. Factory preset calibrations cannot be
changed in the field.
Location 0 (DIESEL1) is factory calibrated for solvent extraction testing of refined oils.
(calibrated against diesel)
Location 1 (CRUDE1) is factory calibrated for solvent extraction testing of crude oils.
Location 2 (DIESEL2) is factory calibrated for direct water samples of refined oils.
(calibrated against diesel)
Location 3 (CRUDE2) is factory calibrated for direct water samples of crude oils.
Locations 4 to 9 are available for customer site or oil specific calibrations.
5.1 SOLVENT EXTRACTION TECHNIQUE
If calibrating or testing using the Solvent Extraction Mode, your water samples will need to be solvent
extracted for the calibration and all ensuing tests.
If not using a Solvent Extraction method, proceed to Section 5.2.
The following explains the solvent extraction technique that will be used for calibration and testing in the
Solvent Extraction Mode.
EPA 1664 Rev A uses Hexane and ISO 9377-2 uses Pentane in their solvent extraction procedures.
Since this is a correlation instrument, either solvent or alternative non-fluorescing solvents can be used.
Be consistent: Choose a solvent for your calibration and use the same solvent for all testing.
ISO and EPA methodologies recommend lowering the water pH to < 2.0 for sample preservation and
improved release of solubles. If this is desirable, add 10 ml of HCL acid to your water sample and
shake well prior to adding the solvent.
Since this is a correlation instrument, the use of HCL is your option. Be consistent: If you add HCL to
your calibration sample, you should add HCL to all sample testing.
The following is the standard procedure for solvent extracting oil from water. See appendix A for more
detailed information.
1. For the calibration procedure fill two jars; one for the calibration and one to send to your lab.
Fill the sample jar or a glass graduated cylinder to 100 ml of the water to be tested (or 90 ml water + 10
ml HCL). This will be for your calibration. Fill a second jar full (no air), use HCL if required, tighten the
lid and send to your lab. Some labs will provide water sample kits to use.
2. Add 10 ml of solvent to your calibration jar using the Solvent dispenser. Do not add solvent to the
jar tagged for the lab.
3. Close the lid on the jar and shake the sample for 2 minutes.

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4. Rest the jar on a table to settle for 2 minutes. The solvent will separate and rise to the surface with
the extracted oils.
5. Tap the jar lightly on the table to release any trapped bubbles or emulsions.
6. Insert a disposable pipet so the tip is halfway into the solvent layer and extract some solvent into the
pipet bulb.
7. Fill the appropriate cuvet to about 3/4 full.
8. Wipe the cuvet with clean tissue prior to testing.
5.2 LED INTENSITY SET-UP
The Fluorocheck II uses a UV LED that is driven by current. The more current, the more intense the
LED. Different oil types yield different fluorescence energy. Refined and light oils yield lower
fluorescence energy and need more UV light. Crude and heavy oils have higher fluorescence energy
and need less UV light. The current for the UV LED can be set from 0-25.5 mA.
The Calibration Locations of 4-9 can each have their own LED intensity assigned.
The Factory default intensity is set at 10 mA but to enhance a site specific calibration, it is
recommended to maximize the LED intensity for your sample. To determine the best intensity, obtain a
sample of contaminated water with an approximate known concentration. Ideally, source a sample with
a concentration that is at the high range of your expected routine tests.
If you are calibrating for a solvent extraction Mode, you will need to perform a solvent extraction of the
water sample for this LED intensity test. Refer to 5.1 above for the solvent extraction procedure.
Press < SETUP>, then Press <4> for Diagnostics
The current LED Power is displayed. Choose the cuvet for the Mode you are calibrating to.
Solvent Extraction of Refined Oils: 12mm Diameter (2 MIL) cuvette
Solvent Extraction of Crude Oils: 4 mm Diameter (200 uL) cuvet (use cuvet holder)
Sample Screening (Direct Water) of Refined Oils: 12 mm Diameter (2 MIL) cuvet
Sample Screening (Direct Water) of Crude Oils: 12 mm Diameter (2 MIL) cuvet
With your water or extracted sample in the appropriate cuvet, insert it into the FluoroCheck. Close the
lid. The fluorescence (FLR) response will be indicated in mV.
The maximum mV response of this instrument is 2,000 mV. If your sample is at the high range of
expected routine values, change the LED mA to achieve a mV of about 1500. If your sample
concentration is about 50% of your expected range of readings, change the LED mA to achieve a mV
reading of about 1,000.
When the best intensity is determined, record this LED mA value to be used during the calibration
procedure.
5.3 SITE CALIBRATION
When a site specific calibration is performed, one sample of clean water plus up to four samples of
different concentrations can be used to make the internal calibration curve.

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The first sample must be oil-free (0) water, preferably the process water.
Although the unit can be calibrated using only one other sample of process contaminated water,
additional samples will improve the calibration accuracy. For calibrating, choose sample concentrations
that will be typical of routine samples.
For example, if typical samples range from 0 to 50 mg/l or ppm, a calibration value of 40 would be
appropriate. If sample concentrations of 10 mg/l or ppm are more common, calibration samples
around 10 or 15 would be more appropriate.
If the sample concentrations are unknown during calibration, reserve an identical sample for analysis by
your local lab. A temporary value can be used during calibration and corrected later.
Using a process contaminated water sample for calibration will provide the most reliable calibration
rather than a manually prepared sample. Preparing a sample with water and oil may not be indicative
of actual process conditions of oil type, oil dispersion, and background contaminants. Prepared
samples may be unstable and retention of oil injected into the water can be difficult. If preparing your
own standard, use glass containers only (plastic containers draw oil out of the water). Use a carrying
agent, such as acetone, to help disperse the oil into the water prior to the extraction. A method of
preparing samples is available in Appendix A.
5.4 CALIBRATING THE INSTRUMENT
Press <SETUP>, enter password 2000 <ENTER>. Press 1 for Units. Select ppm or mg/l for the
display and calibration units.
Press the <CAL> key; then Press <2> to perform new calibration
CALIBRATION
1-Select from list
2-Perform new cal
Current: xxxxxx
Select the Test Mode that you are calibrating for.
Select Mode First
1 Solvent Extraction
2 Sample Screening
NEW CALIBRATION
xxxxxx xxxxxxx
For: Cal4
Press 4-9 then enter
Select a Calibration Location from # 4 to # 9 to store your calibration, then press <ENTER>. Locations
0-3 are reserved for Factory Calibrations and cannot be changed. The screen will display the following:
Depend on Select Mode

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Current LED: xx.xmA
Change LED Power?
No=Press<Enter> key
Yes=Any other key
If you are accepting the mA intensity value as displayed, Press <ENTER>. If you have determined a
new LED Intensity value for your sample from section 5.2 above, press any other key to proceed, key
in the new value and press <ENTER> .
NEW CALIBRATION
For: Cal6
1-Auto 2-Manual
Current LED: xx.xmA
1 – Auto will calibrate the unit based on the user presently having contaminated water samples ready
(the concentration value may be known or unknown).
2 – Manual will allow you to later view and change the concentration and mV values that were entered
during the Auto calibration mode. This is used to correct any temporary values that were entered while
waiting for laboratory results of true values.
4th line shows LED power: This indicates the LED intensity you have chosen.
Press <1> for Auto.
Auto CAL: Cal4
Enter=Continue-Pt 1
0=Calibration
1stpt:Must be 0 ppm
1. The first calibration point must be oil-free water or a solvent sample. Press <Enter> to proceed.
Fill the appropriate cuvet for your chosen mode to about 3/4 full with clean water or the pure solvent of
choice and insert into the FluoroCheck. Cal point 1: 0 will be displayed along the the raw fluorescence.
Wait a few seconds for this reading to stabilize and then Press <ENTER>.
2. The display will now prompt to enter Cal point 2. Press <Enter> to continue with Point 2 calibration.
If doing a Solvent Extraction Calibration, the oil/water sample must be extracted with solvent first.
Enter the known oil concentration of your contaminated water sample to be calibrated . Do Not Press
<Enter> yet. If the value is not known, enter a suspected temporary value. This can be corrected after
a lab test of your sample.
Fill the appropriate cuvet to about 3/4 full with the sample and insert into the FluoroCheck. The raw
fluorescence (FLR value) is displayed (The FLR value should be greater than Cal point 1) Wait a few
seconds for this reading to stabilize and then Press <ENTER>.

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If the value was unknown, label saved lab jar of this water sample as Point #2. Send it to the lab for
analysis. Do not send the sample with solvent in it. If using HCL acid, send a sample with HCL added.
The display will now prompt to enter Cal point 3. If another sample is available to increase the points
on the calibration curve, press <ENTER> to continue and repeat the above procedures for each
sample.
If no more sample points are to be entered press <0> for Calibration Done. If the FLR value were the
same between points then a “No Change in Signal” error will occur. If the same ppm value was used
between Points the “Identical ppm” will display
Press the <DISPLAY> key to exit the Calibration Menu.
1) If the sample values were known during calibration, Go to 5.5. to complete the calibration.
2) If the samples were unknown and have been sent to a lab, Stage 1 Calibration is complete.
You can use the unit with the temporary values while waiting for the test results.
When the results are received from the lab, the temporary values need to be corrected.
Press the <CAL> key, enter the password then press <ENTER>
Press <2> for Perform new Cal. Enter previous mode of extraction. Confirm the Calibration Location is
the same as originally selected, then press <ENTER>. Press <ENTER> to proceed without LED
changes.
Press <2> for Manual
The values from your original calibration have been held in memory and will be displayed.
Press <ENTER> to view the First Point 0 ppm.
Press <ENTER> to accept the First Point FLR reading.
Press <ENTER> to view the 2nd ppm value entered. Key in the correct ppm or mg/l value received
from the laboratory.
Press <ENTER>.
Press <ENTER> to accept the Second Point FLR reading.
Follow this procedure for any further calibration points entered or press <0> for Calibration done.
Press the <DISPLAY> key to exit the Calibration Menu. Go to Section 5.5. to complete the
calibration.
5.5 SETTING THE REFERENCE CUVET
You can now assign a value to your Reference Cuvet.
Crude Oil: REF A
Refined Oils (e.g. Diesel): REF B

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A Reference Cuvet will allow frequent re-calibrations and checks without having to use prepared
samples. Insert the supplied Reference Cuvet into the testing well. Always match the dots on the
cuvet holder and testing well. Close the lid. If in the Solvent Extraction or sample Screening Mode, a
reading is displayed.
Record the displayed or average reading for future calibrations. This value is specific to your
calibration. It is not indicative of other hydrocarbon types or sites.
Periodic re-calibrations using actual prepared or analyzed samples may be desired to maintain a good
correlation against local laboratory results.