Bel Bio2 User manual

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BIOLOGICAL MICROSCOPE

Mod. BIO2 binocular / trinocular

DIN160mm optics

USER MANUAL

Filename: Bel Photonics BIO2_User manual_REV1.5.doc

Bel Engineering srl

Electronic precision balances and scientific instruments

Via Venezia Giulia 1, 20052 Monza (MI) Italia

tel:+39 039 2006102/2005302 fax: +39 039 2006082

e.mail: [email protected] www.belengineering.com

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The model BIO2 biological microscope is equipped with a set of achromatic (or semiplan or

plan-achromatic) objectives and 10x (optional 16x) wide field eyepieces. The observer can get the

clear image in the wide field. It’s suitable for scientific research, medical health work and teaching

demonstration in the colleges.

I . SPECIFICATIONS

1.Eyepieces

Type Magnification Focus(mm) Field(mm) Remark

Wide field

eyepiece 10X 25 18

Plan eyepiece 16X 15.6 11 Optional

2.Objectives

Type Magnification Numerical aperture

Working distance

(mm)

4X 0.1 37.4

10X 0.25 6.6

40X 0.65 0.64

Achromatic

100X (oil) 1.25 0.19

4X 0.1 17.9

10X 0.25 8.8

40X 0.65 0.56

Plan

achromatic

100X (oil) 1.25 0.33

3.Total Magnification

Total Objectives

Magnification

Eyepieces

4X 10X 40X 100X

10X 40X 100X 400X 1000X

16X 64X 160X 640X 1600X

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4.Condenser numerical aperture: NA=1.25.

5.Stage cross travel range: longitudinal 35mm traverse 75mm.

6.Interpupillary distance adjustment range: from 55mm to 75mm.

7.Light sources :

6V 20W (optional 30W) halogen lamp, brightness can be adjusted.

AC/DCadapter:output6V5A

8. Collector with diaphram for Koehler illumination

9. Nosepiece inward tilted. Macro and micrometric focus regulations (step 0,002 mm) with coaxial

knobs. Friction and height limit adjustable

10.Anti-fungus.

Electrical and physical Specifications:

Power supply External switching, 100-230 V, 50-60 Hz

Output 6V

Max power absorbed 35 Watt

Dimensions (mm) (LxPxH) 195x320x400

weight 6 Kg

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II . COMPONENTS

Fig.1

Binocular

Limiting

holding knob Nosepiece

Objective

Stage

Coarse

focusing knob

Fine

focusing knob

Condense

up-down knob

Tube holding screw

Eyepiece

Collecto

holding screw

Collector

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Trinocular tube

(model BIO2T)

Fig.2

Adjustable

tensional knob

Lengthwise knob

Specimen holder

Cross knob

Power switch

brightness

control

Condense

holding screw

AC/DC

ada

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III . INSTALLATION

Eyepiece

Binocular

Tube

holdin

screw

Nosepiece

Objective

Condenser with

erture dia

hra

ter seat

Fig.3

Trinocular tube

(mod. BIO2T)

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IV . MICROSCOPE OPERATIONS

1.Adjustment of interpupillary distance

Put the specimen on the stage and ring the specimen into exact focus. Adjust the interpupillary

distance of binocular until the right-left field of view can be composed one. (See Fig.4)

2.Adjustment of diopter

Put the specimen on the stage. Turn the 40X objective to working position. Firstly, observe at right

tube with right eye, adjust coarse-fine focusing knob to image clearly. Secondly, observe at left tube

with left eye, adjust the diopter control 1 to image clearly.

3.Switching and luminosity regulation

With reference to figure 5: press the switching button 1 and regulate the luminosity control until

the image is enough clear.

Note: Do not leave into the maximum luminosity position the light control since it may reduce the

lamp life.

Fig.4

Fig.5

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4. Coarse and fine focusing.

The instrument uses coaxial coarse/fine focusing mechanism. With reference to figure 6: the

knob 3a is for macrometric focus, the knob 3 for the fine focus.. --- After positioning the specimen

onto the stage, first act on the coarse focus knob to get near the focalization plane, then optimize

the focus of the image through the fine focus knob.

The lever 4 is for adjusting the tension of the coarse focusing knob to prevent the stage from

naturally sliding down; regulate this according to your preferences and requirements. The lever 5 is

the limiting lever and prevents accidental specimen-objective contact; unblock the lever, bring the

specimen stage to a end of stroke position suitable for your requirements and then block again the

lever.

5. Condenser positioning

With reference to figure 7: the condenser can be displaced up and down acting on the knob 6. It

can also be easily removed from its support unscrewing its central holding screw. The side screws

9 allow the centering of the position of condenser (see section V.a). The opening of the condenser’s

aperture diaphragm can be regulated acting on the lever 7, rotating this to the right and to the left.

Regulating the opening of the aperture diaphragm you can obtain an appropriate contrast (see

section V.a). The filter seat 8 allows the placement of color round filter in the optical light path.

6.Specimen stage

3a 4

Fig.6

Fig.7

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With reference to figure 8: the clamp 10 on the specimen plane is used for holding down the

specimen 11. Knobs 12 and 13 regulate the position of specimen stage along longitudinal and

transversal directions.

7.Trinocular tube (only for models BIO2-T)

With reference to figure 9: trinocular tube 15 allows to attach, through the optional adapters, both

reflex cameras and hi-resolution CCD and CMOS cameras. Through the screw 16 remove the

protection cap and then, through the correct adapters with C-mount thread, attach your camera.

For 1/3’’ sensor cameras it is advisable to use 0,4X C-mount adapter.

Fig.8

Fig.9

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V. GETTING READY FOR OBSERVATION

a) PROCEDURE TO SET UP THE MICROSCOPE FOR KOEHLER ILLUMINATION

1. Switch on the microscope through the switching button placed on the base of microscope,

check that light reaches correctly the objectives.

2. Select objective 10X.

3. Completely open the diaphragm (A) of the illuminator and the diaphragm of the condenser

(B).

4. If the condenser is equipped with the additional lens (swing lens) or if a round filter (diffusion

filter) is placed in the filter seat of the condenser, rotate this out and take care that the

swing lens or the filter seat with filter is completely removed from the optical path.

5. Take a specimen and place it on the specimen stage.

6. Watching in the eyepieces, use the focusing knobs (D) to move the specimen stage up and

down until you obtain a clear image of the specimen. It may happen that the final image is

not perfect since the optimization process isn’t finished yet.

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7. Now close the field diaphragm (A) of the illuminator about half of its total aperture and,

through the appropriate side knob (C), regulate the height of condenser until the image of

the contour (or part of it) of the field diaphragm with its sheets can be observed clearly, like

in fig.10.

8. If the image of field diaphragm is not centered in the field of view, proceed to its centering

acting on the appropriate side condenser centering screws (E) until the image of field

diaphragm is brought into the center of the field of view, like in fig.11.

9. At this point, to fine adjust the centering of the condenser, open the illuminator field

diaphragm (A) up to the contour of field of view and verify that the dark edge all around is

equidistant from the edge of the field of view. Act on the appropriate centering screw until

you obtain the best result.

10.Now open completely the illuminator field diaphragm (A) so that its sheets are not anymore

visible in the field of view. The condenser is now centered and at the correct working height

and it is not necessary to center it again subsequently (unless that some changes are made

on the condenser position).

The microscope is now correctly set to work with the best illumination

on the specimen (Köehler illumination).

11.To finish illumination optimization, it is advisable to regulate the condenser aperture

diaphragm (B) to obtain the best resolution and the best image contrast for the

magnification selected. At this point you must close the condenser diaphragm, which was

completely open at the beginning. However it must not be never completely closed since

diffraction effects may reduce resolution of image details.

Fig.10

Fig.11

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Fig. 12

CAMERA OR

OTHER DEVICE

WITH C MOUNT

THREAD

TRINOCULAR

TUBE

ADAPTER WITH C

MOUNT THREAD

Adjusting the condenser diaphragm you can find the best compromise between resolution,

contrast and depth of field for the selected objective.

When another objective is selected, you should always adapt the condenser aperture

diaphragm. In particular, the more you go towards higher magnifications the more you must

close the condenser’s diaphragm; this is done to permit that light cone coming out from

condenser can fit at the best the numerical aperture of the selected objective.

To find the best regulation for every objective of the microscope, it is enough to watch the

specimen image and, as well, close the condenser’s diaphragm until the image starts

becoming a little darker and also richer in contrast. However it is advisable not to close the

diaphragm more than 1/3 of its total aperture to avoid that diffraction effects may reduce

the resolution and then defocus fine details of the image or introduce artifacts on the image.

With these regulations, you obtain the best compromise among resolution, contrast and

depth of field for the selected objective.

It is advisable to repeat procedure for Koehler illumination and contrast optimization

every time you start using your microscope,

in order to achieve the best observation results.

b) USE OF IMMERSION LIQUIDS WITH 100X OBJECTIVE

The 100X objective with numerical aperture 1,25 must be used with the immersion oil, in order to

achieve the optical performance for which the objective was designed.

Take the oil bottle supplied with the microscope (use only the oil supplied or one of same chemical

characteristics) and place one drop upon the specimen (trying to avoid air bubbles). At this point,

bring the 100X objective, put into the work position and make the frontal lens of the objective to

adhere with the oil drop; thus creating an uniform region formed by specimen, oil and 100X

objective frontal lens.

c) INSTALLATION OF DEVICES WITH C MOUNT INTO MICROSCOPE TRINOCULAR TUBE

(for Mod. BIO2-T Trinocular)

With reference to FIG.12

To effect this operation it is necessary the adapter with thread

for C mount (optional)

Unscrew the screw that holds the protection cap of trinocular

tube and remove the cap. Screw the C mount adapter into the

device (camera or CCD sensor) you want to use. Now put the

device with the adapter over the trinocular tube and fix it with

the screw.

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VII. HALOGEN LAMP REPLACEMENT

With reference to Fig.13:

1) Switch off the microscope power supply.

2) Tilt the microscope, loose the screw 3 fixing the lamp base board 4 in the middle

part of the bottom of base. Open the lamp base board 4.

3) Remove the old lamp 1 from its support 2.

4) Insert the new lamp in its support 2, handling it carefully, using gloves or a piece

of paper to avoid touching it directly with fingertips ( direct contact with skin can

deteriorate the performance of the illuminator and shorten the life of the lamp).

5) Put back the base board 4 and fix it with screw 3.

6) Once placed you must center the lamp in its housing to have the maximum light entering the

illuminator and so a clearer image. To do this, insert the power supply and switch on the

microscope. Rotate the 4X objective in working position, align the condenser and check that

light path is correct. Look in the field of view if the light is decentered, then unscrew the screw 5

and act on it to shift the position of the lamp. Center the lamp and then tighten again the screw.

VIII. Maintenance

1. Clean the lenses

Sweep the lens by lens tissue or soft fabric immersed with mixed liquid of alcohol/ether or

diethyl benzene.

3 4

Fig.13

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2. Cleaning of painted parts

The dust on the painted parts can be removed by gauze, for the grease spots, the gauze

immersed slightly with aviation gasoline is recommended. Do not use organic solvents such as

alcohol, ether or other thinner etc, for cleaning the pointed parts or plastic components.

3. Avoid disassembling the microscope

Microscopes are precise instrument, do not disassemble it casually since it may cause serious

damage to its performance.

4. Being not used

Cover the microscope with organic glass or polyethylene and places where there is dry and

modules. Suggest that storage all objectives and eyepieces in closed container with drying

agent.

VIII. PROBLEMS AND SOLUTIONS

TYPE OF PROBLEM PROBLEM SOLUTION

1 Check plug is well connected Plug properly

A. Lamp not working

2 Check lamp is not broken Change lamp

1 Check that ABBE condenser is not

out of optical path

Place the Abbe condenser in center of

optical path

2 Check if filter-seat is positioned

properly Put the filter seat in right position

B. Something in the field of

view

Check if additional lens (in models

where this lens is present) of

condenser is positioned properly

Insert completely the lens or remove it

completely from optical path

C. Images are not

visualized when acting on

focus

See section

“IV. MICROSCOPE OPERATIONS ” and

follow instrunctions

D．Image not clear on

focus plane

Check that eyepieces, condenser and

illuminator do not have dust

Read section “VII.MAINTENANCE”; and follow

instructions. If lenses have been damaged

please contact us in order to repair the

microscope.

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