BEL BIO2 User manual

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[Bel Engineering holds the right to make technical or other modifications to its products without prior warning] Bel Photonics BIO2_User manual_REV1.5.doc
BIOLOGICAL MICROSCOPE
Mod. BIO2 binocular / trinocular
DIN160mm optics
USER MANUAL
Filename: Bel Photonics BIO2_User manual_REV1.5.doc
Bel Engineering srl
Electronic precision balances and scientific instruments
Via Venezia Giulia 1, 20052 Monza (MI) Italia
tel:[phone] fax: [phone]
e.mail: [email protected] www.belengineering.com

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[Bel Engineering holds the right to make technical or other modifications to its products without prior warning] Bel Photonics BIO2_User manual_REV1.5.doc
The model BIO2 biological microscope is equipped with a set of achromatic (or semiplan or
plan-achromatic) objectives and 10x (optional 16x) wide field eyepieces. The observer can get the
clear image in the wide field. It’s suitable for scientific research, medical health work and teaching
demonstration in the colleges.
I . SPECIFICATIONS
1.Eyepieces
Type Magnification Focus(mm) Field(mm) Remark
Wide field
eyepiece 10X 25 18
Plan eyepiece 16X 15.6 11 Optional
2.Objectives
Type Magnification Numerical aperture
Working distance
(mm)
4X 0.1 37.4
10X 0.25 6.6
40X 0.65 0.64
Achromatic
100X (oil) 1.25 0.19
4X 0.1 17.9
10X 0.25 8.8
40X 0.65 0.56
Plan
achromatic
100X (oil) 1.25 0.33
3.Total Magnification
Total Objectives
Magnification
Eyepieces
4X 10X 40X 100X
10X 40X 100X 400X 1000X
16X 64X 160X 640X 1600X

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[Bel Engineering holds the right to make technical or other modifications to its products without prior warning] Bel Photonics BIO2_User manual_REV1.5.doc
4.Condenser numerical aperture: NA=1.25.
5.Stage cross travel range: longitudinal 35mm traverse 75mm.
6.Interpupillary distance adjustment range: from 55mm to 75mm.
7.Light sources :
6V 20W (optional 30W) halogen lamp, brightness can be adjusted.
AC/DCadapter:output6V5A
8. Collector with diaphram for Koehler illumination
9. Nosepiece inward tilted. Macro and micrometric focus regulations (step 0,002 mm) with coaxial
knobs. Friction and height limit adjustable
10.Anti-fungus.
Electrical and physical Specifications:
Power supply External switching, 100-230 V, 50-60 Hz
Output 6V
Max power absorbed 35 Watt
Dimensions (mm) (LxPxH) 195x320x400
weight 6 Kg

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[Bel Engineering holds the right to make technical or other modifications to its products without prior warning] Bel Photonics BIO2_User manual_REV1.5.doc
II . COMPONENTS
Fig.1
Binocular
Limiting
holding knob Nosepiece
Objective
Stage
Coarse
focusing knob
Fine
focusing knob
Condense
r
up-down knob
Tube holding screw
Eyepiece
Collecto
r
holding screw
Collector

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Trinocular tube
(model BIO2T)
Fig.2
Adjustable
tensional knob
Lengthwise knob
Specimen holder
Cross knob
Power switch
brightness
control
Condense
r
holding screw
AC/DC
ada
p
te
r

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III . INSTALLATION
Eyepiece
Binocular
Tube
holdin
g
screw
Nosepiece
Objective
Condenser with
a
p
erture dia
p
hra
gm
F
il
ter seat
Fig.3
Trinocular tube
(mod. BIO2T)

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[Bel Engineering holds the right to make technical or other modifications to its products without prior warning] Bel Photonics BIO2_User manual_REV1.5.doc
IV . MICROSCOPE OPERATIONS
1.Adjustment of interpupillary distance
Put the specimen on the stage and ring the specimen into exact focus. Adjust the interpupillary
distance of binocular until the right-left field of view can be composed one. (See Fig.4)
2.Adjustment of diopter
Put the specimen on the stage. Turn the 40X objective to working position. Firstly, observe at right
tube with right eye, adjust coarse-fine focusing knob to image clearly. Secondly, observe at left tube
with left eye, adjust the diopter control 1 to image clearly.
3.Switching and luminosity regulation
With reference to figure 5: press the switching button 1 and regulate the luminosity control until
the image is enough clear.
Note: Do not leave into the maximum luminosity position the light control since it may reduce the
lamp life.
Fig.4
1
Fig.5
1
2

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4. Coarse and fine focusing.
The instrument uses coaxial coarse/fine focusing mechanism. With reference to figure 6: the
knob 3a is for macrometric focus, the knob 3 for the fine focus.. --- After positioning the specimen
onto the stage, first act on the coarse focus knob to get near the focalization plane, then optimize
the focus of the image through the fine focus knob.
The lever 4 is for adjusting the tension of the coarse focusing knob to prevent the stage from
naturally sliding down; regulate this according to your preferences and requirements. The lever 5 is
the limiting lever and prevents accidental specimen-objective contact; unblock the lever, bring the
specimen stage to a end of stroke position suitable for your requirements and then block again the
lever.
5. Condenser positioning
With reference to figure 7: the condenser can be displaced up and down acting on the knob 6. It
can also be easily removed from its support unscrewing its central holding screw. The side screws
9 allow the centering of the position of condenser (see section V.a). The opening of the condenser’s
aperture diaphragm can be regulated acting on the lever 7, rotating this to the right and to the left.
Regulating the opening of the aperture diaphragm you can obtain an appropriate contrast (see
section V.a). The filter seat 8 allows the placement of color round filter in the optical light path.
6.Specimen stage
3
3a 4
5
Fig.6
6
9
7
8
Fig.7

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With reference to figure 8: the clamp 10 on the specimen plane is used for holding down the
specimen 11. Knobs 12 and 13 regulate the position of specimen stage along longitudinal and
transversal directions.
7.Trinocular tube (only for models BIO2-T)
With reference to figure 9: trinocular tube 15 allows to attach, through the optional adapters, both
reflex cameras and hi-resolution CCD and CMOS cameras. Through the screw 16 remove the
protection cap and then, through the correct adapters with C-mount thread, attach your camera.
For 1/3’’ sensor cameras it is advisable to use 0,4X C-mount adapter.
Fig.8
12
10
13
11
15
16
Fig.9

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V. GETTING READY FOR OBSERVATION
a) PROCEDURE TO SET UP THE MICROSCOPE FOR KOEHLER ILLUMINATION
1. Switch on the microscope through the switching button placed on the base of microscope,
check that light reaches correctly the objectives.
2. Select objective 10X.
3. Completely open the diaphragm (A) of the illuminator and the diaphragm of the condenser
(B).
4. If the condenser is equipped with the additional lens (swing lens) or if a round filter (diffusion
filter) is placed in the filter seat of the condenser, rotate this out and take care that the
swing lens or the filter seat with filter is completely removed from the optical path.
5. Take a specimen and place it on the specimen stage.
6. Watching in the eyepieces, use the focusing knobs (D) to move the specimen stage up and
down until you obtain a clear image of the specimen. It may happen that the final image is
not perfect since the optimization process isn’t finished yet.
E
B
C
A
B
D