BioLife SU105UE User manual

U LT 25NEU
O perating
M anual 86ºC
Ultra-Low
Temperature
Freezer

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U LT 25NEU Quick Guide
INITIAL SETUP
Port Stopper/
Sealant !The Port Stopper/Sealant must be in place during operation
Power Indicator AThe unit is ON when lit
Power ON BPress and hold for two beeps
Power OFF BPress and hold for three beeps
LCD Display CDisplays chamber temperature
Display Setpoint FPress/Release to display current setpoint, S ## displayed
Return to Temp
Display FPress/Release or wait eight seconds
Change Setpoint FPress and hold until Set T LED Eglows, S ## displayed,
then ^or vD
ADVANCED FUNCTIONS
Change
to Preset Press and hold Set Funtil Set T LED Eglows,
S ## displayed, then select Preset 1, 2 or 3 J
Postpone
Over Temperature
Alarm
When Over Temp LED Iglows, press Preset 3 J
until time shows (1.0 h) (If Over Temp LED is not glowing,
then Preset 3 acts as a Temp Preset)
Return to
Temp Display Wait eight seconds, press Preset 3 to return to temperature display
See Error
Code Again If Error LED His glowing, press Preset 2 Jbriefly
(If Error LED is not glowing, then Preset 2 becomes Temp Preset)
Lock Panel Press and hold Preset 1 AND 2 AND 3 Juntil Locked
LED Gturns off (~five seconds)
Unlock Panel Press and hold Preset 1 AND 2 AND 3 Juntil Locked
LED Gturns off (~five seconds)
LCD Display
Codes P ## – Preset Temp, ## h – Alarm Silenced, E ## – Error Code (see operating manual)

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A
B D F
C E G H I
J
NOTICE:
When unit is first turned on red Over Temp LED Iwill be on until temperature
is within° of setpoint
Panel cannot be locked until temperature is within ° of setpointPanel cannot
be locked until temperature is within º of set point
ULT25NEU QUICK GUIDE (Continued)

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U LT25NEU
86C PORTABLE ULTRALOW TEMPERATURE FREEZER
The ULTNEU (UltraLow Temperature  liter volume) incorporates next
generation freepiston Stirling engine technology Freepiston Stirling engine
technology differs from conventional compressorbased refrigeration in
that it provides high efficiency deeptemperature cooling in a lightweight
package allowing true portable operation
TABLE OF CONTENTS
1. SAFETY PRECAUTIONS 6
1.1 Injury Prevention 
1.2 Damage Prevention 
1.3 Transportation Care 
1.4 Flammable Refrigerant Use 
2. UNPACKING AND SETUP 9
3.1 Pictorial Tour Freezer 
3. FEATURES OF THE
ULT25NEU FREEZER 11
3.2 Pictorial Tour Control Panel 
3.3 Power Cords 
3.4 Intended Uses 
4. OPERATION 15
4.1 How to Turn the Freezer On/Off 
4.1.1 To Turn the Freezer On 
4.1.2 To Turn the Freezer Off 
4.2 Changing the Set Temperature 
4.2.1 To See the Current
Set Temperature 
4.2.2 Change the Setpoint
to a New Value 
4.3 Using Preset Temperatures 
4.4 OverTemperature Alarm 
4.4.1 How to Silence the Audible
Temperature Alarm 
4.4.2 To Postpone an Alarm 
4.5 Error Conditions 
4.6 Locking the Control Panel 
4.7 How to Switch Between
Power Supplies 
5. ERROR CODES 20
6. MAINTENANCE 21
6.1 Caring for Your Freezer Gasket 
6.2 Air Intake Filter 
6.3 Storage 
6.4 Cleaning 
7. TROUBLESHOOTING 22
8. CALIBRATION GUIDE 23
9. SPECIFICATIONS 26
9.1 Freezer Specifications 
10. WARRANTY 30

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To prevent personal injury, injury to others, or damage to property,
read these safety precautions carefully before use.
1.1 INJURY PREVENTION
Do not cut change or modify the power cable*
When removing the plug hold onto the plug and not the cord
An ultralow temperature freezer is qualitatively different than a home freezer
At ºC frostbite can occur instantly
The user should establish and follow a protocol for safe ultralow temperature
operating procedures This should include (but not limited to)
>Never handle samples or freezer accessories with bare hands
>Do not use gloves which become brittle at ultralow temperatures
>Nitrile and latex gloves are inadequate
Permeable gloves are dangerous because ultralow temperature materials
can contact skin and cause damage
>Be especially careful that materials at ultralow temperature are not spilled
onto skin or clothing
>Use only sample containers that have been approved or tested for ultralow
temperature use
>Some plastics shatter at ultralow temperatures Avoid splinter hazards
>Biological and chemical hazards are still hazardous at ultralow temperature
Always wear proper protective equipment and follow appropriate
isolation protocols
>Many types of labels will fall off and/or break at ultralow temperature
Some types of ink which stick to glass and/or plastic at room temperature
lose adhesion at ultralow temperature
In addition to the ultralow temperature hazards above there are also physical
hazards to consider
>Be cautious when closing the lid to avoid a pinching hazard
>Be careful when loading the box with heavy items
>Always use the handles to carry the freezer
1. SAFETY PRECAUTIONS

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1.2 DAMAGE PREVENTION
Do not disassemble modify or repair There are no user serviceable parts
inside the freezer unit*
Do not immerse in water or pour water on the unit*
Do not put ice or liquid water directly in the freezer box always use suitable
containers
Do not use glass containers when the contents might freeze and break
Do not store flammable items such as gasoline thinner or solvents in the
freezer The freezer is NOT rated as an explosionproof freezer
Do not use hard and/or sharp objects such as knives screwdrivers etc
to remove any frost or ice which has accumulated on the inside of the freezer
The inside panels are heat exchangers and can be damaged
Do not block the air intake or air discharge vents
Do not drop throw or abuse the freezer*
Do not operate under extreme environmental conditions such as in a car
trunk in very high humidity environments in rain or other severe weather*
Do not use solvents to clean the control panel or the outside or inside
of the freezer
*Warning: Unauthorized modification to the cabinet, controls or free-piston
Stirling engine is prohibited and will void all warranty provisions.
1. SAFETY PRECAUTIONS (Continued)

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1. SAFETY PRECAUTIONS (Continued)
1.3 TRANSPORTATION CARE
Use only factoryprovided packaging If unavailable contact the
manufacturer for replacement packaging materials
Do not place unit on sides or turn upside down
1.4 FLAMMABLE REFRIGERANT USE
ULTNEU uses  grams of R (Ethane) in a hermetically sealed
thermosiphon tubing It requires caution in use and repair
A Danger – Risk of Fire or Explosion Flammable Refrigerant Used
Do Not Drill or Puncture Inner Liner 
B Danger – Risk of Fire or Explosion Flammable Refrigerant Used
To be Repaired Only by Trained Service Personnel  Component Parts Shall
Be Replaced With Like Components Do Not Puncture Refrigerant Tubing
C Caution – Risk of Fire or Explosion Flammable Refrigerant Used
Consult Repair Manual/Owner’s Guide Before Attempting to Service
this Product  All Safety Precautions Must be Followed
D Caution – Risk of Fire or Explosion Dispose of Properly in Accordance
with Federal or Local Regulations  Flammable Refrigerant Used
E Caution – Risk of Fire or Explosion Due to Puncture of Refrigerant Tubing
Follow Handling Instructions Carefully  Flammable Refrigerant Used

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2. UNPACKING AND SETUP
Remove the freezer and all accessories from the box. Carefully
inspect the freezer and all accessories for any shipping damage.
Check the packing list to verify that the shipment is complete.
Ensure the access port stopper or sealant is in place.
Place the freezer on a level surface.
Make sure that the air inlets and outlets are not blocked.
 Connect to a power source. While the freezer can be used
with either the AC Power Cord for lab, home or office use,
or the DC Power Cord for mobile use, it is recommended that
the initial pull-down to Setpoint be completed via AC power.
A To use the AC Power Supply
iDuring setup identify the service power and
plug configuration available and then locate
the appropriate line cord for the service type
you will be using
ii If the appropriate line cord is not currently installed
simply unplug the line cord from the unit and then
plug the appropriate line cord in its place
iii Plug the other end of the line cord into the power
source and then attempt to power on the unit
B To use the DC Power Cord in a motor vehicle
iMake sure the freezer remains level Exceeding
an angle of  degrees may cause loss of cooling
ii Plug the DC Power Cord into a V outlet that is
rated at  amps NOTE Not for use with  volt
automotive systems
NOTE Not for use with  volt automotive systems
 A DC REQUIREMENT Consult your automotive
specialist if your vehicle lacks  A rated V outlets
iii Plug the opposite end into the freezer Slide the male line cord adapter
into the female connection port lining up the two plastic bungs on
the male adapter with the cutouts in the female port of the freezer
Press the male adapter firmly into the connection port and turn
clockwise until an audible “click” indicates full connection
Power Cord
Available Separately.

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 Notes on operation in a vehicle.
A The freezer will operate on vehicle battery power for a limited time before
the battery is drained and the motor vehicle cannot be started This time
will vary from vehicle to vehicle but is generally only a few hours
B The vehicle engine should be running to prevent accidental total discharge
of the battery
C The power to V outlets in some vehicles may be interrupted during engine
starting This will cause a sudden stop of the cooling unit which may produce
a sound that is different than when turning off the freezer by using the
On/Off button This is normal and will not damage the cooling engine
The cooling engine will restart automatically once power resumes
D 20 A DC REQUIREMENT: Please note that  A DC service is required for
proper functioning of the freezer in a motor vehicle Some vehicles may not
be equipped with  A DC service Consult your automotive specialist to
verify the level of DC service installed in your vehicle
E Do not operate the freezer in an unattended vehicle This may lead to
overheating if left in the sun and subsequent damage to stored contents
 Disconnecting from power.
A Turn off the freezer (Press and hold On/Off button for three beeps)
iThe LCD display will show the word “OFF” while the unit turns off
ii Unplug the freezer after the word “OFF” disappears and the display
goes dark
iii If you unplug the freezer while it is on you will hear a sudden noise as
the freepiston Stirling engine shuts off Power outages will also cause the
same noise This noise (described as a “bonk”) is not indicative of damage
2. UNPACKING AND SETUP (Continued)

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3.1 PICTORIAL TOUR, FREEZER
The Stirling Ultracold ULTNEU freepiston Stirling engine
ultralow temperature freezer has the following features
 A freezer chamber which is protected by two lids
> Inner lid has a tight fitting foam lid
 Control Panel
> Described in more detail on page 
 Lid Latch
> For positive closure
 Access Port
> For thermocouple wires etc
 Cleanable Filter
> Helps protect the heat rejection fins from dust
 AC Power Connection
> Polarized to avoid error
 DC Power Connection
 Temperature Probe Clip (optional)
> For holding the temperature probe in
place for calibration and monitoring
 Remote Alarm Contacts (optional)
 Key Lock
> Locking lid for sample integrity
 Power Supply Fan
 Vent
3. FEATURES OF THE ULT25NEU FREEZER










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3. FEATURES OF THE ULT25NEU FREEZER (Continued)

 
Outer Lid:
double gasket
moisture seal.
AC Power Connection. DC Power Connection.
Inner Lid:
high-density,
closed cell,
rigid foam.
Power Cord Available
Separately.
Power Cord
Available Separately.
Access Port:
1/4” (6.3 mm).
Temperature Probe Clip
(optional).
Slide-out Filter:
easy to clean, protects
heat rejection fins.
Remote Alarm
Contacts (optional).







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3.2 PICTORIAL TOUR, CONTROL PANEL
AOn/Off Indicator LED
BOn/Off button
> Turns freezer on and off
CLCD display
> Alpha/numerical display default display is chamber temperature
D^/v buttons
> Used to adjust Setpoint Temperature (when the display shows S ##)
ESet Temp LED
> Temperature can be adjusted when lit
FSet Temp button
> This must be pressed to adjust the Set Temperature
GLocked LED
> Indicates when the control panel is locked
HError LED
> Indicates when there is an electrical mechanical or similar error condition
IOver Temp LED
> Indicates when the chamber is over/under the setpoint
The audible beeper can be set to delay warning
JTemperature Preset buttons
A
B D F
C E G H I
J
3. FEATURES OF THE ULT25NEU FREEZER (Continued)

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3.3 POWER CORDS
A simple line cord change can allow for multiple power and receptacle types
See Section  for directions
Make sure to use the cord and plug appropriate for your location
Power supply options include:
North America VAC Hz NEMA P plug and VAC Hz
NEMA P plug
Europe/International V AC Hz Standard twopin plug
3.4 INTENDED USES
The ULTNEU freezer provides ultralow temperature storage for both
medical and nonmedical purposes The storage of blood or blood products
intended for medical purposes is prohibited
3. FEATURES OF THE ULT25NEU FREEZER (Continued)

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Important changes to the freezer settings require sustained button pushes
This helps prevent accidental changes to the settings during transportation
Some sequences require a threesecond button press and hold while other
sequences require a fivesecond button press and hold
For example
Turning the freezer ON requires a threesecond press and hold (two beeps)
Turning the freezer OFF requires a fivesecond press and hold (three beeps)
The default display is the chamber temperature Other displays such as
Setpoint Alarm Postpone etc are marked with a letter such as S (Setpoint)
h (Alarm Postpone) etc These secondary displays revert to the default
display after approximately eight seconds of button inactivity
4.1 HOW TO TURN THE FREEZER ON/OFF
4.1.1 To Turn the Freezer On
Press and hold the On/Off button for two beeps (~three seconds)
The freezer will display a greeting message and then display the
chamber temperature
4.1.2 To Turn the Freezer Off
Press and hold the On/Off button for three beeps (~five seconds)
The freezer will display “OFF” for  seconds and then go dark
4.2 CHANGING THE SET TEMPERATURE
4.2.1 To See the Current Set Temperature
Press the Set Temp button briefly
The current set temperature will be displayed with the letter S ##
>After eight seconds of inactivity the display will revert to the
current temperature
4. OPERATION

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4.2.2 Change the Setpoint to a New Value
There are two ways to change the Setpoint
>Manually adjust the temperature using ^/v
>Use one of the three adjustable Preset Buttons
Adjusting the Setpoint manually
>Because of the importance of the Setpoint Temperature changing
the Setpoint requires a “twobutton procedure” This means
Method A – press/hold the Set Temp button while simultaneously
pressing ^/v
OR
Method B – press/hold the Set Temp button until the Set Temp LED
glows and then press ^/v
(Methods A and B achieve the same effect The user can use
whichever method they prefer)
To change the Setpoint by Method A
>Press and hold the Set Temp button and then push the ^/vbuttons
(do not release the Set Temp button)
The Setpoint temperature will appear with the prefix S in the LCD display
When the ^/vbuttons are first pushed the Setpoint will change by
ºC at first
If the ^/vbuttons are held for five seconds the step size will increase/
decrease by ºC
If the ^/vbuttons are briefly released (less than a half second) while
continuing to hold down the Set Temp button the increment/decrement
size will return to ºC
Release all buttons once the desired Setpoint is achieved The display
will return to chamber temperature after about eight seconds
4. OPERATION (continued)

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To change the Setpoint by Method B
>Press and hold the Set Temp button until the Set Temp LED glows
The Setpoint temperature will appear with the letter S in the first
LCD display
>The ^/vbuttons can be used to change the Setpoint
When the ^/vbuttons are pushed the Setpoint will change by ºC at first
If the ^/vbuttons are held for five seconds the step size will increase
to ºC
Briefly release (a half second) the ^/vbuttons while continuing to
hold down the Set Temp button the increment/decrement size will
return to ºC
Release the ^/vbuttons once the desired Setpoint is achieved
>Press the Set Temp button briefly (less than one second) to turn off
the Set Temp LED The display will return to chamber temperature
4.3 USING PRESET TEMPERATURES
The Temperature Presets —   and  can be used to quickly change the
Setpoint to frequently used values When shipped the Presets are set to
ºC ºC and ºC
To choose one of the preset temperatures
>Press and hold the Set Temp button until the Set Temp LED glows
>Push one of the Temperature Preset buttons briefly
>The new Set Temperature will be shown in the LCD display
>If this is the desired Set Temperature the user can either
Briefly press the Set Temp button to turn off the Set Temp LED
OR
Wait for approximately eight seconds when the freezer control panel
will return to normal operation
4. OPERATION (continued)

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4.4 OVERTEMPERATURE ALARM
If the freezer compartment temperature is ºC warmer or colder than
the Setpoint for five seconds then it will go into temperature alarm mode
>The Over Temp LED will turn on (this is used for under temperature
alarms as well)
>The freezer will make a double beep every  seconds
>Situations which can affect the freezer’s ability to maintain a steady
state or cooling rate include
The lid is ajar
The air vents are blocked
Addition of a large relatively warm mass
Power supply problems
Mechanical and/or electrical problems
4.4.1 How to Silence the Audible Temperature Alarm
The Over Temp LED will always be lit when the freezer’s temperature
is over or under the set temperature by more than ºC
4.4.2 To Postpone an Alarm
When the audible alarm is sounding it can be postponed
Press the Temperature Preset – 3 button (located directly beneath the
Over Temp LED) and the audible alarm will be turned off for  hour
4.5 ERROR CONDITIONS
Certain conditions will cause an Error Code to be displayed on the LCD
display and the Error LED will be lit
>The error code will be of the form E ## where ## is the error code
See Section  for possible error codes
>The error code will be displayed for about five seconds and then the
display will show the chamber temperature The Error LED will stay on
>The error code can be recalled by briefly pressing the Temperature
Preset – 2 button located directly under the Error LED This will recall
the error code for approximately  seconds
>If the Temperature Preset – 2 button is pressed and held for five seconds
(three beeps) this will clear both the LCD display and the Error LED
However if the error condition persists the error cycle will start over again
4. OPERATION (continued)

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4.6 LOCKING THE CONTROL PANEL
To Lock the Freezer Control Panel
Press and hold the Temperature Preset 1, 2 and 3 buttons until the
freezer beeps three times
>The Locked LED will light up
>The control panel cannot be locked if there is an over temperature
or other error condition
To Unlock the Freezer Control Panel
Press and hold the Temperature Preset 1, 2 and 3 buttons until the freezer
beeps three times
>The Locked indicator LED will turn off
>The freezer will still indicate overtemperature alarms or error conditions
while it is locked The user has to unlock the control panel to address
those alarms/conditions
4.7 HOW TO SWITCH BETWEEN POWER SUPPLIES
See Section  for possible power sources for the ULTNEU
To change from one power supply to another
>Turn OFF the freezer (Press and hold the On/Off button)
>Wait for the freepiston Stirling engine to slow and stop ( seconds)
>Unplug the freezer from the current power source and plug into the new
approved power source Reference Section  Part B for instructions
on use of a DC Power cable
>The freezer can be restarted immediately no wait time necessary
>Turn ON the freezer by pressing and holding the On/Off button
4. OPERATION (continued)

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These are displayed in the LCD display as E ##
 The freepiston Stirling engine is not maintaining a working temperature
This could arise from blocked filters blocked air passages unusually hot
ambient temperatures (for example >ºC) or a fan failure Check air
passages and clear any blockages for good air circulation Remove from
hot environments (car interiors etc)
 A component which monitors freepiston Stirling engine performance has
failed The freepiston Stirling engine cannot regulate itself Please return
for repair
5. ERROR CODES