CORNING Axygen GD-1000 User manual

A Corning Brand
Axygen®Gel Documentation
Systems
Instruction Manual
Catalog Numbers:
GD-1000
GDBL-1000

Table of Contents
1.0 Introduction ...................................................................................1
2.0 Safety Information .............................................................................1
2.1 Symbols and Conventions ...................................................................1
2.2 UV Safety Precautions.......................................................................1
2.3 Electrical Safety Precautions.................................................................1
3.0 Package Contents ..............................................................................2
4.0 Technical Specifications.........................................................................2
5.0 System Placement..............................................................................2
6.0 Axygen® Gel Documentation System Installation Guide ..........................................3
7.0 Axygen Gel Documentation System-BL Installation Guide ........................................5
8.0 Image Acquisition ..............................................................................6
9.0 Overview of Software Features..................................................................6
10.0 Accessories (sold separately) ....................................................................9
11.0 Limited Warranty...............................................................................9
12.0 Equipment Disposal ............................................................................9

Instruction Manual | 1
1.0 Introduction
The Axygen® Gel Documentation System (GD-1000) and Axygen Gel Documentation System-BL (GDBL-1000) easily capture publication
quality, 16 bit TIFF images. The systems are simple to set up and have a straightforward user interface for image capture, annotation,
and contrast adjustment. Images are easily saved and opened in common gel analysis software for more detailed analysis.
2.0 Safety Information
Read these instructions before operating the Axygen Gel Documentation System and Axygen Gel Documentation System-BL. This
instrument is suitable for general laboratory use only. Therefore, it must be used only by personnel who know the health risks
associated with the reagents that are normally used with this instrument.
2.1 Symbols and Conventions
Protective Earth Terminal
The earth terminal, intended for connection to an external protective conductor for protection against electric shock in
case of a fault, is located on the inside of the back panel.
Hot Surface Warning
Under normal conditions, the temperature of glass surface of UV transilluminator is below 50°C and safe to touch.
However, if the system malfunctions, it is possible that the glass surface temperature exceeds 80°C. Please exercise
caution when touch the glass surface with your hand when this occurs.
2.2 UV Safety Precautions
UV CAUTION Ultraviolet (UV) radiation can cause serious damage to unprotected eyes
and skin. We recommend wearing UV glasses or a face shield.
The Axygen Gel Documentation systems come with a built-in ultraviolet (UV 200 to 400 nm) transilluminator inside the system.
Exposure to UV radiation can cause permanent damage to the eyes and skin. The system enclosure confines the radiation within the
system and shields the user from exposure. The system is also equipped with a two-way safety interlock switch that automatically
cuts off the power to the transilluminator when the door is open during normal use.
Always wear UV-protective eyewear that is specified by the manufacturer as providing protection at the wavelength(s) used, making
sure that the eyewear protects any areas where radiation may come through (UV sunglasses may not prevent UV radiation from
coming in through the sides or around the lenses).
Always cover all skin that may be exposed to UV light, especially the face, neck, hands, and arms.
Always make sure that any UV protection devices (such as the safety switch on the light cabinet apparatus) are working properly.
If not, discontinue use until the device(s) are properly repaired.
Use only UV lamps in the transilluminator.
2.3 Electrical Safety Precautions
Be sure to take proper precautions when handling any electrical equipment. NEVER work on any live circuit, fixture, receptacle, or
switch. Safety rules you should follow whenever working with any electrical appliance include:
Always shut off power at the main disconnect before changing a fuse.
Always shut off power to the circuit before repairing or replacing a switch, receptacle, or fixture.
Always tape over the main switch, empty fuse socket, or circuit breaker you are working on.
Always check that the circuit is dead before beginning work on it. Using a circuit tester or voltmeter can help you determine this.
Always unplug any appliance before repairing it.
Voltage Setting Information
The Axygen Gel Documentation systems have a power supply that automatically chooses the correct voltage for your country or
region. Protection category: IP20 according IEC 60529.

2| AXYGEN GEL DOCUMENTATION SYSTEMS
3.0 Package Contents
Axygen® Gel Documentation System includes:
Axygen Gel Documentation system cabinet, including the
camera and orange filter
A-to-A male USB cable
Axygen test target
Axygen USB (includes imaging software, calibration files)
3 power cables 110V/220V (US/EU/UK). Please select the
correct one for your region.
Axygen Gel Documentation System-BL includes:
Axygen Gel Documentation System-BL cabinet, including the
camera and orange filter
Tablet computer (includes imaging software, calibration files)
Axygen test target
3 power cables 110V/220V (US/EU/UK). Please select the
correct one for your region.
4.0 TechnicalSpecications
Axygen Gel Documentation System Axygen Gel Documentation System-BL
Camera 5.4 MP image resolution 5.4 MP image resolution
Lens F1.8 F1.8
Light Sources Epi White
Dual wavelength transillumination at 302 nm
and 365 nm
Epi White
Dual wavelength transillumination at 302 nm
and 365 nm
Epi Blue
Trans White Light
Illumination Tray
Optional Optional
Field of View 15 x 20 cm 15 x 20 cm
Cabinet Door sensor to prevent accidental exposure to UV;
automatically shuts off UV light when door is open
Safety override, enabling band cutting on the UV
transilluminator with automatic turn off after
5 minutes
Door sensor to prevent accidental exposure to UV;
automatically shuts off UV light when door is open
Safety override, enabling band cutting on the UV
transilluminator with automatic turn off after
5 minutes
Emission Filter 590 nm 590 nm
3-position Filter Slider No Yes
Certifications CE, cTUVus CE, cTUVus
Product Footprint 12 x 15 in. (30.48 x 38.1 cm) 12 x 15 in. (30.48 x 38.1 cm)
Computer Operating system: Microsoft® Windows® 10 and above
Minimum hardware requirements: 1.4 GHz processor
speed, 2 GB RAM, 16 GB free hard disk space, 2 USBs
Tablet included: Windows 10 tablet computer enables
compatibility with existing networks, as well as easy
setup with Bluetooth and wireless compatible, or USB
to LAN compatible.
12” touch screen
256 GB of storage for short-term data saving
5.0 System Placement
Make sure the system is located away from regular exposure to water, solvents, or corrosive material. Place on a flat and stable
surface with 10 cm clearance on all sides, including the top, to allow sufficient air flow.
The system is intended for indoor use with the following ambient conditions:
Altitude up to 2,000 m
Temperature 5°C to 40°C
Maximum relative humidity 80% for temperatures up to 31°C decreasing linearly to 50% relative humidity at 40°C
The system should be placed away from interfering electrical signals and magnetic fields. If possible, a dedicated electrical outlet
should be used to eliminate electrical interference from other instrumentation in your laboratory.

Instruction Manual | 3
Remove the Axygen Gel
Documentation system from
its packaging.
1.
Select GD1000, then click
Next.
Select your installation
location, then click Next.
Click Install to continue with
the installation.
Click Next to begin the driver
installation.
To continue, accept the license
agreement. Then click Next.
To continue, accept the license
agreement. Then click Next.
After the installation is
complete, click Finish.
Click Finish to exit the Setup
Wizard.
Identify the Axygen USB and
connect it to a Microsoft®
Windows® 10 computer.
2.
Click Next to begin the Setup
Wizard.
4.
5. 6. 7. 8.
9. 10. 11. 12.
On the USB, find the folder
“Axygen Imaging Files\Axygen
GDS_v_XXX” and open it.
Double click on the Setup file.
(You may see a message pop
up that says “Do you want to
allow the following program
from an unknown publisher
to make changes to this
computer?” Select Yes.)
3.
6.0 Axygen® Gel Documentation System Installation Guide

4| AXYGEN GEL DOCUMENTATION SYSTEMS
Plug the system into an
available outlet.
Connect the A-to-A USB cable
to both the system USB and
the computer. On the Axygen
system, ensure the USB is
plugged into the lower USB
on the right hand side of
the instrument.
Power on the Axygen Gel
Documentation system using
the switch in the back. Wait
about 20 seconds before
proceeding to the next step.
Launch the capture software. The first time you run the
Axygen Capture Software,
you will receive the message
above. Click Allow Access.
13. 14. 15. 16.
17. 18. 19.
Find the “Master” folder
within the “System Calibration
Folder.” Copy the Master
folder, and paste it in
C:\ProgramData\Axygen\
Axygen Imaging.
When asked if you would like
to replace the existing folder,
select Yes.
NOTE: If you cannot see the
Program Data folder, please
make sure you can view
“hidden” files in Windows®.
On the Axygen USB, locate the
folder “Axygen Imaging Files\
System Calibration Folder
AXXXXX.”

Instruction Manual | 5
Remove the Axygen Gel
Documentation system-BL
from its packaging.
Locate the tablet computer. Remove the wing nuts from
the back of the tablet.
Mount the tablet on the
system.
Secure the tablet in place with
the wing nuts.
Connect the mini-USB cable to
the tablet.
Connect the power cable to
the tablet.
Power on the system using the
switch on the back.
Power on the tablet. Launch the capture software.
Plug the system into an
available outlet.
1. 2. 3. 4.
5. 6. 7. 8.
9. 10. 11.
7.0 Axygen® Gel Documentation System-BL Installation Guide

6| AXYGEN GEL DOCUMENTATION SYSTEMS
8.0 Image Acquisition
1. After the acquisition software is launched, select the Darkroom tab.
2. Place your sample in the imager. The capture software will be in “Live” mode to allow you to position your sample.
3. Close the cabinet door.
4. Select your light source.
Here is a quick guide to select your light source:
Axygen® Gel Documentation System Axygen Gel Documentation System-BL
EtBr Gel UV 302 or 365 UV 302 or 365
Sybr Safe Gel UV 302 Epi Blue
Coomassie Gel* Visible Visible
*Requires optional white light tray.
5. Select exposure time. If you are not sure of your exposure time, select Auto Exposure. Otherwise, you can select a time using the
exposure slider.
6. A preview of your image will be displayed. If the image is acceptable, select Image Capture. Otherwise you can continue to modify
the exposure time.
7. When you are done, your image will be displayed in the home tab. You can now save your image.
9.0 Overview of Software Features
Open
Open a previously saved image.
Save
Save allows you to perform one of three
actions:
Save – save the currently active image
Save as – allows you to save the currently
active image under another file name
Save all – saves all open images
Print
Print active image.
@
@
Email
Allows you to email the active image. Please see
“Email Settings” in the Settings section of this
guide.
Resize
An image can be resized up to 3 times its
original size.
Crop
When you select the crop tool, a crop window
will appear over your image. Drag the corners of
the crop window to your desired size, and then
select “Crop ROI” from the bottom of the screen.
If you do not want to crop, select “Cancel.”
Rotate
Rotate allows you to rotate your image one of
four ways:
Right 90 degrees
Left 90 degrees
Flip horizontal
Flip vertical
i
Image Info
Captures some of the information on how your
image was captured, including the date and
time captured, and exposure time. Additionally,
it tells you the light source and calibration
value.
Home Tab

Instruction Manual | 7
Manual Contrast
Allows you to manually adjust the black and
white levels of your image.
Invert
Gives the inverse of the image.
Display Saturation
Saturated pixels are displayed in red.
Darkroom Tab
UV 302
Selects the Trans UV 302 light source.
UV 365
Select the Trans UV 365 light source.
Epi White
Selects the Epi White light source.
Epi Blue
Turns on the Epi Blue light source.
Axygen Gel Documentation System–BL only
Trans White
Optional – with the purchase and calibration of
the white light tray. You select the Trans White
light source.
Slider
Use this slider to select your exposure time. As you change
the time, wait for the live image below to update for the best
experience. Alternatively, you can type in the exposure time in
the text fields to the right, and then select Enter.
Auto Exposure
Calculates an exposure time using the full
dynamic range of the camera.
Show Grid
Displays a grid over the live image so you can
make sure your gel is vertically and horizontally
straight.
Select ROI
Select ROI has two functions:
Select ROI then select Image Capture – this
results in a final cropped imaged
Select ROI then select Auto Exposure – this
results in auto exposure calculations only
influenced by your region of interest.
Image Capture
Select this to capture your image. It will be
displayed in the Home tab for further work.
Band Excision
If you need to open the door and view your gel
with the lights on, you can override the safety
precautions in our system.
NOTE: Always wear protective eyewear when
dealing with UV light. To use the band excision
tool, select your light source then select band
excision. A dialog box will appear and ask you
to pull out the safety switch on the right side
of the system. Open the door, pull the safety
switch, and the light will go on. Select “Band
Excision” again to turn the light off. The light
will automatically turn off after 5 minutes.
Home Tab (continued)
Zoom In
Zoom in on center of image
Zoom Out
Zoom out from center of image
Zoom Fit
Adjusts zoom to fill window. Displays % in the
lower left corner of the window.
Auto Contrast
Calculates a balanced contrast ratio.

8| AXYGEN GEL DOCUMENTATION SYSTEMS
Annotations Tab
The annotations tab lets you annotate or draw on an open image.
Pointer
This is the object selector. It allows selection of
text boxes, objects, lines, etc., in order to edit or
delete them. For example, to change the color
of a box, select the pointer, double click on the
box to be changed, then select the color select
tool and click on the color desired.
Rectangle
Draw a rectangle on the image
Ellipse
Draw an ellipse on the image
Line
Draw a line on the image
Text
Click on the text box, then draw a rectangle.
This rectangle will be where your text appears.
You can change the size of the font after you
type your text.
Pencil
Use this to free-hand draw on the image.
Undo
Use this tool to undo your last few steps.
Redo
Redo your last step.
Delete
Delete the highlighted annotation object.
Font/Color/Line Width
Once an annotation object is selected using
the pointer, you can edit the line width and the
color, and in the case of text, the font.
Settings Tab
The password for settings is admin.
Create Flats
This tool is used to create flat field image
for certain light sources. Flat files are used
to calibrate light sources for the most even
illumination. Flat field calibration is required if
you purchase the White Light Tray accessory.
Instructions for calibration and use are included
with the tray.
@
@
Email Settings
You can email files to yourself if you set up an
email account for the imaging system. Contact
your IT department for the best values to set.
Change Password
The default system password is admin (all
lowercase). If you would like to change this
password, you can do so here.
Help Tab
Allows you to view this Instruction Manual, save it, or print it.
Enable Trans White
When optional White Light Tray is purchased,
enables Trans White to appear as an option in
the darkroom.

Instruction Manual | 9
10.0 Accessories (sold separately)
Products may not be available in all markets.
Cat. No. Description
GD-BLCS Gel Documentation System blue light conversion screen for GD-1000
GD-WLCS Gel Documentation System white light conversion screen for GD-1000 and GDBL-1000
GD-GCT Gel carrying tray for GD-1000 and GDBL-1000
GDBL-PRT Thermal printer
GD-PAPER Thermal printer paper
GD-1D 1 user license of Total Lab 1D (more user licenses available upon request)
GD-GCS UV transmitting tray that protects UV transilluminator when cutting out bands
GD-OG Orange glasses ideal for reducing background when cutting out bands with blue light
GD-USBS USB hub
GD-USBMK USB mouse and keyboard
11.0 Limited Warranty
Corning Incorporated (Corning) warrants that this product will be free from defects in material and workmanship for a period of
two (2) years from date of purchase. CORNING DISCLAIMS ALL OTHER WARRANTIES WHETHER EXPRESSED OR IMPLIED, INCLUDING
ANY IMPLIED WARRANTIES OF MERCHANTABILITY OR OF FITNESS FOR A PARTICULAR PURPOSE. Corning’s sole obligation shall be to
repair or replace, at its option, any product or part thereof that proves defective in material or workmanship within the warranty
period, provided the purchaser notifies Corning of any such defect. Corning is not liable for any incidental or consequential damages,
commercial loss or any other damages from the use of this product.
This warranty is valid only if the product is used for its intended purpose and within the guidelines specified in the supplied
Instruction Manual. This warranty does not cover damage caused by accident, neglect, misuse, improper service, natural forces or
other causes not arising from defects in original material or workmanship. This warranty does not cover consumables, wearing part
(objective protective cover), or damage to paint or finish. Claims for transit damage should be filed with the transportation carrier.
In the event this product fails within the specified period of time because of a defect in material or workmanship, contact Corning
Customer Service at: USA/Canada 1.800.492.1110, outside the U.S. +1.978.442.2200, visit www.corning.com/lifesciences, or contact
your local support office.
Corning’s Customer Service team will help arrange local service where available or coordinate a return authorization number and
shipping instructions. Products received without proper authorization will be returned. All items returned for service should be sent
postage prepaid in the original packaging or other suitable carton, padded to avoid damage. Corning will not be responsible for
damage incurred by improper packaging. Corning may elect for onsite service for larger equipment.
Some states do not allow limitation on the length of implied warranties or the exclusion or limitation of incidental or consequential
damages. This warranty gives you specific legal rights. You may have other rights which vary from state to state.
No individual may accept for, or on behalf of Corning, any other obligation of liability, or extend the period of this warranty.
For your reference, make a note of the serial and model number, date of purchase, and supplier here.
Serial No. __________________________________________ Date Purchased _______________________________________
Model No. _________________________________________ Supplier _____________________________________________
12.0 Equipment Disposal
According to Directive 2012/19/EU of the European Parliament and of the Council of 4 July 2012 on waste electrical and
electronic equipment (WEEE), this product is marked with the crossed-out wheeled bin and must not be disposed of with
domestic waste.
Consequently, the buyer shall follow the instructions for reuse and recycling of waste electronic and electrical equipment
(WEEE) provided with the products and available at www.corning.com/weee.

Warranty/Disclaimer: Unless otherwise specified, all products are for research use or general laboratory use only.* Notintended for
use in diagnostic or therapeutic procedures. Not for use in humans. These products are not intended to mitigate the presence of
microorganisms on surfaces or in the environment, where such organisms can be deleterious to humans or the environment. Corning
Life Sciences makes no claims regarding the performance of these products for clinical or diagnostic applications. *For a listing of US
medical devices, regulatory classifications or specific information on claims, visit www.corning.com/resources.
Corning’s products are not specifically designed and tested for diagnostic testing. Many Corning products, though not specific for
diagnostic testing, can be used in the workflow and preparation of the test at the customers discretion. Customers may use these products
to support their claims. We cannot make any claims or statements that our products are approved for diagnostic testing either directly
or indirectly. The customer is responsible for any testing, validation, and/or regulatory submissions that may be required to support the
safety and efficacy of their intended application.
For a listing of trademarks, visit www.corning.com/clstrademarks. All other trademarks are the property of their respective owners.
© 2015, 2023 Corning Incorporated. All rights reserved. 8/23 CLS-AN-321DOC REV1
Corning Incorporated
Life Sciences
www.corning.com/lifesciences
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