cytiva Amersham ImageQuant 800 User manual

Amersham ImageQuant 800
Operating Instructions
Original instructions
Amersham
ImageQuant 800
cytiva.com

Table of Contents
1Introduction ........................................................................................................ 4
1.1 Important user information ....................................................................................................................... 5
1.2 About this manual ......................................................................................................................................... 6
1.3 Associated documentation ....................................................................................................................... 8
1.4 Abbreviations .................................................................................................................................................. 9
2 Safety Instructions ............................................................................................. 10
2.1 Safety precautions ........................................................................................................................................ 11
2.2 Labels ................................................................................................................................................................. 20
2.3 Emergency procedures ............................................................................................................................... 24
3 System description ............................................................................................ 26
3.1 Introduction ..................................................................................................................................................... 27
3.2 Illustration of the system ............................................................................................................................ 28
3.3 Instrument description ............................................................................................................................... 29
3.4 Amersham ImageQuant 800 control software ................................................................................. 34
3.5 Accessories ..................................................................................................................................................... 42
3.6 Upgrade options ............................................................................................................................................. 44
4 Installation .......................................................................................................... 47
4.1 Site requirements .......................................................................................................................................... 48
4.2 Instrument setup ........................................................................................................................................... 52
4.3 Amersham ImageQuant 800 control software installation .......................................................... 53
4.4 Moving the Amersham ImageQuant 800 ............................................................................................ 54
4.5 System setup ................................................................................................................................................... 55
4.5.1 Connections .................................................................................................................................................... 56
4.5.2 Amersham ImageQuant 800 control software ............................................................................... 59
4.6 Network ............................................................................................................................................................. 65
5 Operation ............................................................................................................ 69
5.1 Start the Amersham ImageQuant 800 ................................................................................................. 71
5.2 Prepare the instrument ............................................................................................................................... 74
5.3 Image capture ................................................................................................................................................. 86
5.4 Shut down the Amersham ImageQuant 800 ..................................................................................... 90
6 Maintenance ....................................................................................................... 95
6.1 Cleaning before planned service ............................................................................................................. 97
6.2 Instrument ........................................................................................................................................................ 98
6.3 Accessories ...................................................................................................................................................... 99
6.4 User maintenance ......................................................................................................................................... 101
7 Troubleshooting ................................................................................................. 102
7.1 Instrument problems ................................................................................................................................... 103
7.2 Image problems ............................................................................................................................................. 105
7.3 Software problems ........................................................................................................................................ 106
7.4 Error messages ............................................................................................................................................... 107
Table of Contents
2 Amersham ImageQuant 800 Operating Instructions 29372604 AC

8 Reference information ....................................................................................... 109
8.1 Specifications .................................................................................................................................................. 110
8.2 Recycling information .................................................................................................................................. 113
8.3 Regulatory information ............................................................................................................................... 115
8.3.1 Contact information .................................................................................................................................... 116
8.3.2 European Union and European Economic Area .............................................................................. 117
8.3.3 Eurasian Economic Union
Евразийский экономический союз .................................................................................................. 118
8.3.4 Regulations for North America ............................................................................................................... 120
8.3.5 Regulatory statements .............................................................................................................................. 121
8.3.6 Declaration of Hazardous Substances (DoHS) ................................................................................ 122
8.3.7 Other regulations and standards .......................................................................................................... 124
8.4 Health and Safety Declaration Form ...................................................................................................... 125
Index ........................................................................................................................... 127
Table of Contents
Amersham ImageQuant 800 Operating Instructions 29372604 AC 3

1 Introduction
About this chapter
This chapter contains important user information, descriptions of safety notices, regu-
latory information, and a general description of the intended use of Amersham™
ImageQuant™ 800.
In this chapter
Section See page
1.1 Important user information 5
1.2 About this manual 6
1.3 Associated documentation 8
1.4 Abbreviations 9
1 Introduction
4 Amersham ImageQuant 800 Operating Instructions 29372604 AC

1.1 Important user information
Read this before operating the
product
All users must read the entire Operating Instructions before installing, oper-
ating or maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not install, operate, or perform maintenance on the product in any other way than
described in the user documentation. If you do, you may be exposed or expose others
to hazards that can lead to personal injury and you may cause damage to the equip-
ment.
Intended use of the product
The Amersham ImageQuant 800 is a CCD camera system that produces digital images
of samples in gels, membranes, plates, and petri dishes in three different modes: chem-
iluminescence, colorimetric, and fluorescence.
The Amersham ImageQuant 800 is intended for research use only and must not be
used in any clinical procedures or for diagnostic purposes.
Prerequisites
In order to operate Amersham ImageQuant 800 in the way it is intended, the following
prerequisites must be fulfilled:
•The user must read and understand the Safety Instructions chapter in the Oper-
ating Instructions.
•The user must have general laboratory experience.
•Amersham ImageQuant 800 must be installed in accordance with the site require-
ments and instructions in the Operating Instructions.
•The instrument must be installed by a Cytiva representative or a third party author-
ized by Cytiva.
Third party intellectual property
With regards to third party patents related to sample preparation etcetera, it is recom-
mended that you consult a lawyer or patent attorney for information on obtaining
appropriate licenses.
1 Introduction
1.1 Important user information
Amersham ImageQuant 800 Operating Instructions 29372604 AC 5

1.2 About this manual
Purpose of this manual
The Operating Instruction manual provides information needed to install, operate and
maintain the product in a safe way.
Scope of this manual
The Operating Instructions cover the Amersham ImageQuant 800 instrument,
Amersham ImageQuant 800 control software, and Amersham ImageQuant 800
CONNECT software.
If you work in a regulated environment and have purchased and installed Amersham
ImageQuant 800 GxP license for Amersham ImageQuant 800 control software, refer
to Amersham ImageQuant 800 GxP User Manual (29620391) for additional informa-
tion as software functions can vary.
The illustration below shows the Amersham ImageQuant 800 system.
Note: Depending on your system configuration, not all options described in this
manual may be available. It is possible to upgrade your system to increase
the range of imaging options. See Section 3.6 Upgrade options, on page 44
for more information.
1 Introduction
1.2 About this manual
6 Amersham ImageQuant 800 Operating Instructions 29372604 AC

Typographical conventions
Software items are identified in the text by bold italic text.
Hardware items are identified in the text by bold text.
In electronic format, references in italics are clickable hyperlinks.
Notes and tips
Note: A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Tip: A tip contains useful information that can improve or optimize your proce-
dures.
1 Introduction
1.2 About this manual
Amersham ImageQuant 800 Operating Instructions 29372604 AC 7

1.3 Associated documentation
Introduction
This section describes the user documentation that is delivered with the product, and
how to find related literature that can be downloaded or ordered from Cytiva.
System-specific documentation
The user documentation listed in the table below is available on the web in PDF format.
Documentation Main contents
Amersham ImageQuant 800 Operating
Instructions
Instructions needed to install, operate,
and maintain the Amersham
ImageQuant 800 in a safe way.
Amersham ImageQuant 800 User
Manual
Additional detailed information and
instructions for the Amersham
ImageQuant 800 instrument,
Amersham ImageQuant 800 control
software, and Amersham ImageQuant
800 CONNECT.
Amersham ImageQuant 800 GxP User
Manual
Additional detailed information and
instructions for the Amersham
ImageQuant 800 system with
Amersham ImageQuant 800 GxP
license.
Amersham ImageQuant 800 Privacy
and Security Manual
Descriptions of the privacy and security
aspects of the Amersham ImageQuant
800 system.
Amersham ImageQuant 800 GxP
Privacy and Security Manual
Descriptions of the privacy and security
aspects of the Amersham ImageQuant
800 system with Amersham
ImageQuant 800 GxP license.
1 Introduction
1.3 Associated documentation
8 Amersham ImageQuant 800 Operating Instructions 29372604 AC

1.4 Abbreviations
Introduction
This section explains abbreviations that appear in the user documentation for
Amersham ImageQuant 800.
Abbreviations
Abbreviation Definition (English) Translation
CCD Charge-coupled device Charge-coupled device
IR Infra-Red Infra-Red
NIR Near infra-red Near infra-red
NP Non-parallax Non-parallax
OD Optical densiometry Optical densiometry
OS Operating system Operating system
PC Personal computer Personal computer
RGB Red Green Blue Red Green Blue
SNOW Signal to Noise Optimization Watch Signal to Noise Optimization Watch
UV Ultra-violet Ultra-violet
1 Introduction
1.4 Abbreviations
Amersham ImageQuant 800 Operating Instructions 29372604 AC 9

2 Safety Instructions
About this chapter
This chapter describes safety precautions, labels and symbols that are found on the
equipment. In addition, the chapter describes emergency and recovery procedures.
In this chapter
Section See page
2.1 Safety precautions 11
2.2 Labels 20
2.3 Emergency procedures 24
Important
WARNING
Before installing, operating or maintaining the product, all
users must read and understand the entire contents of this
chapter to become aware of the hazards involved.
2 Safety Instructions
10 Amersham ImageQuant 800 Operating Instructions 29372604 AC

2.1 Safety precautions
Introduction
Amersham ImageQuant 800 is powered by mains voltage and handles materials that
can be hazardous. Before installing, operating, or maintaining the system, you must be
aware of the hazards described in this manual.
Follow the instructions to avoid injury to the operator or other personnel,
damage to samples or other substances handled by the equipment, to the
product, or to other equipment in the area.
The safety precautions in this section are grouped into the following categories:
•General precautions
•Personal protection
•Flammable liquids and explosive environment
•Installing and moving the system
•Power supply
•Operation
•Maintenance
Definitions
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to
proceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
2 Safety Instructions
2.1 Safety precautions
Amersham ImageQuant 800 Operating Instructions 29372604 AC 11

General precautions
WARNING
Do not operate the product in any other way than described in the
user documentation.
WARNING
Only properly trained personnel may operate and maintain the
product.
WARNING
Accessories. Use only accessories supplied or recommended by
Cytiva.
WARNING
Do not use Amersham ImageQuant 800 if it is not working prop-
erly, or if it has suffered any damage, for example:
•damage to the power cord or its plug
•damage caused by dropping the equipment
•damage caused by splashing liquid onto it
WARNING
Do not block vents and make sure that they are kept free of dust
and dirt. Blockage can cause overheating and malfunctioning. To
allow adequate cooling make sure there is at least 40 cm of free
space in front of the instrument, at least 30 cm on the right, and at
least 10 cm on left, rear and top sides between walls or other equip-
ment.
CAUTION
Do not lean on the instrument door or the side door.
2 Safety Instructions
2.1 Safety precautions
12 Amersham ImageQuant 800 Operating Instructions 29372604 AC

Personal protection
WARNING
Hazardous substances. When using hazardous chemicals, take
all suitable protective measures, such as wearing protective
clothing, glasses and gloves resistant to the substances used.
Follow local and/or national regulations for safe operation and
maintenance of the product.
CAUTION
Always use appropriate Personal Protective Equipment (PPE)
during operation and maintenance of Amersham ImageQuant
800.
•Protective eyewear
•Protective gloves
•Protective footware, preferably with steel lining
CAUTION
The instrument is equipped with a safety interlock. If the interlock is
out of order or has been tampered with, UV NIR, and visible light
may be emitted, which can cause skin burn and impair vision.
Do not open the instrument door during operation, when the RUN
indicator is lit. If the door is opened and the instrument keeps
running, turn off the instrument immediately and contact Cytiva
service.
Flammable liquids and explosive
environment
WARNING
Flammable liquids. This product is not approved for handling
liquids under conditions where they could be flammable.
Installing and moving the product
WARNING
The product must be installed and prepared by Cytiva personnel or
a third party authorized by Cytiva.
2 Safety Instructions
2.1 Safety precautions
Amersham ImageQuant 800 Operating Instructions 29372604 AC 13

WARNING
Heavy equipment. Contact a Cytiva service person before lifting or
transporting the instrument.
CAUTION
Amersham ImageQuant 800 must be secured before transport or
relocation. Contact Cytiva for instructions before transporting the
instrument.
CAUTION
The product is designed for indoor use only.
CAUTION
Make sure that the system is placed on a stable, level bench with
adequate space for ventilation.
NOTICE
Any computer used with the equipment must comply with EN/IEC
60950-1, and be installed and used according to the manufactur-
er's instructions.
Power supply
WARNING
Supply voltage. Before connecting the power cord, make sure
that the supply voltage at the wall outlet corresponds to the
marking on the instrument.
WARNING
Connect the power supply directly to a grounded wall power outlet.
The use of extension cords or multiple loads on one electrical outlet
could result in fire and electric shock.
2 Safety Instructions
2.1 Safety precautions
14 Amersham ImageQuant 800 Operating Instructions 29372604 AC

WARNING
Power cord. Only use power cords with approved plugs delivered
or approved by Cytiva.
WARNING
Protective ground. The product must always be connected to a
grounded power outlet.
WARNING
Access to power cord with plug. Do not block access to the
power cord. The power cord with plug must always be easy to
disconnect.
NOTICE
If the mains power supply is unreliable, an Uninterruptable Power
Supply (UPS) must be used.
Operation
WARNING
Never exceed the operating limits stated in this document and on
the system label. Operation of the product outside these limits can
damage equipment and cause personal injury or death.
WARNING
Do not use the instrument within or near a sink, or in humid (above
70% of relative humidity) or dusty environments. This can result in
fire or electric shock.
WARNING
In case of thunderstorm, do not touch the power supply plug, as
this can result in electric shock.
2 Safety Instructions
2.1 Safety precautions
Amersham ImageQuant 800 Operating Instructions 29372604 AC 15

CAUTION
Changing the filters must be done using the Amersham
ImageQuant 800 control software procedure. Do not manually
rotate the filter turret.
CAUTION
Do not use the instrument in a room with a temperature above
28°C. Higher temperatures do not allow the CCD to cool down
properly (to -25°C).
CAUTION
Do not insert or spill any substances into the ventilation hole on the
side of the instrument.
CAUTION
Reagents used to prepare the sample should be used in accord-
ance with the manufacturer's instructions.
CAUTION
Use the handle to open or close the side door.
CAUTION
Handle the sample trays and NP lens with care to avoid dropping
them.
CAUTION
Do not place heavy objects on top of the instrument.
CAUTION
Do not touch the light source in the instrument. The light source
may be hot.
2 Safety Instructions
2.1 Safety precautions
16 Amersham ImageQuant 800 Operating Instructions 29372604 AC

CAUTION
Do not leave samples in the instrument after exposure. If left, these
may degrade and cause damage to the instrument.
CAUTION
Do not disable the interlock of the equipment as UV light can be
emitted if the door is opened.
NOTICE
Do not add and/or remove any files in the folder generated by
Amersham ImageQuant 800 with Amersham ImageQuant 800
control software and Amersham ImageQuant 800 CONNECT. Do
not modify any folders in which files have been added or removed.
This may prevent correct image capture and analysis afterwards.
NOTICE
USB ports cannot be used for charging devices.
NOTICE
Connection of multiple computers to one instrument may impair
performance. Simultaneous use can cause loss of data.
NOTICE
Avoid connecting additional accessories such as computers, moni-
tors or keyboards to the instrument during operation as this can
stop the exposure or cause loss of data.
NOTICE
Do not turn off power during operation as this can cause loss of
data and damage the internal memory. Only turn off power in an
emergency situation.
2 Safety Instructions
2.1 Safety precautions
Amersham ImageQuant 800 Operating Instructions 29372604 AC 17

NOTICE
Do not open the instrument door during exposure, when the RUN
light indicates operation. This will stop the exposure and data will
not be saved.
NOTICE
Transferring large amounts of files to a connected computer
during operation can impair system performance.
NOTICE
Do not touch the light source windows as scratches or dirt may
impair performance.
NOTICE
When connecting an external monitor to the system, use a monitor
with WXGA or higher resolution. With a low-resolution monitor,
operation buttons cannot be seen.
NOTICE
Remove all traces of acidic solutions to prevent corrosion in the
equipment.
NOTICE
Wear gloves when handling filters to avoid dirtying or scratching
the filter.
Maintenance
WARNING
Decontaminate before maintenance. To avoid personnel being
exposed to potentially hazardous substances, make sure that the
instrument is properly decontaminated and sanitized before main-
tenance or service.
2 Safety Instructions
2.1 Safety precautions
18 Amersham ImageQuant 800 Operating Instructions 29372604 AC

WARNING
Electrical shock hazard. All instrument repairs or modifications
should be performed by service personnel authorized by Cytiva. Do
not open any covers or replace parts unless specifically stated in
the user documentation.
WARNING
Do not use excessive amounts of liquids for cleaning the
Amersham ImageQuant 800 instrument. This may result in instru-
ment malfunction or electric shock.
WARNING
Hazardous chemicals during maintenance. When using
hazardous chemicals for system cleaning, wash the system with a
neutral solution in the last phase or step.
CAUTION
To reduce the risk of dust entering the turret and affecting the
filters, do not leave the turret door open for long periods of time.
CAUTION
Wear gloves while cleaning. There might be residues of hazardous
chemicals.
CAUTION
Disconnect the power cord before cleaning the inside of the instru-
ment.
NOTICE
Do not use abrasive cleaning materials, such as a scouring pad, for
cleaning. This may cause damage to the instrument.
2 Safety Instructions
2.1 Safety precautions
Amersham ImageQuant 800 Operating Instructions 29372604 AC 19

2.2 Labels
Introduction
This section describes the system label and other safety or regulatory labels that are
attached to the product.
System label
The system label identifies the product and shows electrical data and regulatory
compliance information.
Description of symbols and text on
the system label
The following symbols and text may be present on the system label.
Label Meaning
Warning! Read the user documentation before using the system.
Do not open any covers or replace parts unless specifically stated
in the user documentation.
This symbol indicates that the waste of electrical and electronic
equipment must not be disposed as unsorted municipal waste
and must be collected separately. Please contact an authorized
representative of the manufacturer for information concerning
the decommissioning of equipment.
Frequency
Supply
Voltage
Max.
Current
Electrical requirements:
•Frequency (Hz)
•Supply Voltage (VAC )
•Max. Current (VA)
Alternating current.
Protection
Class
Degree of protection provided by the enclosure.
Manufac-
turing Year
and Month
Year (YYYY) and month (MM) of manufacture.
2 Safety Instructions
2.2 Labels
20 Amersham ImageQuant 800 Operating Instructions 29372604 AC