Denville Scientific M2100 Series User manual

M2100 Series
COMPOUND MICROSCOPE
OPERATION MANUAL
Denville Scientific Inc.
P.O. Box 4588,Metuchen, NJ 07080, USA
Tel: [phone] Fax: [phone]
Toll Free 800-453-0385 www.denvillescientific.com

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Denville M2100 Series Microscope
Thank you for choosing the Denville Scientific M2100 Microscope. This precisely built,
durable microscope will give years of service to even the busiest office practice. Our
technical and customer support departments are ready to assist you with any questions or
comments you may have.
If ever you require an additional accessory or spare part, please contact your local
distributor, or you can contact Denville Scientific for the name of the nearest distributor.
Contents: Pages
Unpacking Denville Scientific M2100 Microscope……………………………… 3
About Denville Scientific M2100 Microscope………………………………….. 3
Objectives ……………………………………………………………….. 3
Oculars (eyepieces) ……………………………………………………... 3
Filter holder………………………………………………………………. 3
Observation Heads ……………………………………………………… 4
Additional M2100 Microscope Features ………………………………… 4
Electronics ……………………………………………………………………… 4
Power Input …………………………………………………………….. 4
Output (illumination)…………………………………………………… 4
Fuse …………………………………………………………………….. 4
Assembly of M2100 Microscope ………………………………………………. 4
Using M2100 Microscope………………………………………………………… 5
Interpupillary Distance………………………………………………….. 6
Focusing Procedures…..………………………………………………… 6
Diopter Adjustments……………………………………………………. 6
Focus Tension Control………………………………………………… 6
Mechanical Stage Upper Limit Setting (Safety Lock)………………….. 6
Lamp Replacement……………………………………………………………….. 7
Fuse Replacement ……………………………………………………………….. 7
Maintenance……………………………………………………………………… 8
Photo with Nomenclature………………………………………………………. 9

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Unpacking Denville Scientific M2100 Microscope
Each Denville Scientific microscope has been packed with utmost care. Please take a
moment to examine the outer and inner cartons for any visual damage. We recommend
that you keep all of the packing material until you have fully assembled, examined and
tested your new microscope. If any parts are missing, please call Denville Scientific customer
service at 800-453-0385 or e-mail us at info@Denville scientificsci.com. Please be ready with
the serial #, date of purchase and name of supplier.
Unpack your M2100 microscope using the following checklist for the parts and
accessories. (Your specific order may vary)
(1) Microscope stand with NA 1.25 Abbe condenser
(1) Head options: (Binocular, or trinocular, or back-to-back dual binocular, or
monocular)
(2) 10x High-Eyepoint eyepieces
(4) Achromatic objectives 4x, 10x, 40xr and 100xr (oil) / (or what you have ordered)
(2) Replacement fuses (1.5A)
(1) Dust cover
(1) Operation manual
If any parts are missing, please contact Denville Scientific customer service at 800-453-
0385 or e-mail us at [email protected]
The DENVILLE M2100 Series Microscope
The Denville Scientific M2100 series microscopes were designed with the user in mind.
Whether it is for use in a busy medical or veterinary practice, a research department, or
for anyone needing the most versatile microscope available, there is a M2100 to meet
your needs.
Objectives:
Each M2100 microscope comes equipped with 4 achromatic DIN flatfield
objectives (optional Semi-plan and Plan objectives are available). The color-
coded, polished objectives are parfocal and parcentered. The 100X (oil) and the
40X (high dry) are spring loaded to prevent damage.
Oculars:
Two Widefield 10X 18mm “High-Eyepoint” eyepieces are included with each
M2100 microscope. The tube size is standard 23.2mm. The specialized High-
Eyepoint eyepieces are designed to reduce eyestrain while wearing glasses.
Filter Holder:

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A swing-out filter holder is installed under the condenser for holding a blue (or
other color) filter to sharpen contrast, which is recommended for routine
hematology or urine analysis.
Head:
The head of your M2100 microscope may be a binocular, trinocular, back-to-back
dual binocular, monocular, or dual viewing head (teaching head). The binocular
and trinocular heads are Seidentoph inclined 45° style. Each can be set for
individual requirements and comfort. The inner diameter of the eyepiece tube size
is standard 23.2mm.
Additional M2100 microscope features:
Large mechanical stage (135mm x 150mm) with low mounted coaxial controls.
The stage upper-movement limit (safety lock) can be set as desired to protect
slides as well as objectives.
Coaxial coarse and graduated fine focusing knobs with tension adjustment control
Focusable Abbe condenser (N.A. 1.25) with iris diaphragm.
Pre-aligned illumination and easy lamp replacement.
Universal power input from 110V/60Hz to 230V/50Hz, detachable power cord.
Electronics
Denville Scientific M2100 microscope uses UL and CSA approved electrical
components. The circuit board in the base of the unit contains all the electrical functions.
There are no user repairable parts on the circuit board.
Power Input:
AC 115V/60Hz - 220V/50Hz universal.
Output:
LED , 3 Watt
Fuse: A 1.5 Amp fuse protects the circuit board from electrical overload. The fuse case
is part of the power inlet socket located in the back of the microscope. When
replacing the fuse, always install a new one of the same size and amperage.
M2100 Microscope Assembly Instructions
1. Remove the stand of the microscope and place it on a sturdy, dust free surface. Set the
base so that the Denville Scientific label faces you.
2. Locate the nosepiece (reference page 9 photo with nomenclature), and remove the
plastic dust plugs. Save these in individual objective containers.

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3. Install the objectives. Each objective is packed in an individual plastic container.
Remove each objective from its container, save the containers in the Styrofoam
carton. Install the objectives in the following order: 4x, 10x, 40x, and 100x. Make
certain that they are screwed in all of the way.
4. Remove the microscope head from the Styrofoam carton. Remove the plastic dust
plugs from the eyepiece tubes as well as the protective plastic cover from the head
(save the protective plastic parts). Plug on base the upper part of the arm and secure
with the retention screw. Note: Don’t over tighten.
5. Unwrap the protective tissue from the eyepieces carefully and slide in each eye tube.
6. Slide holder: The slide holder has already been installed on the mechanical stage. If
you ever need to remove the slide holder, locate the holding screws. Loosen the two
holding screws and remove the slide holder by sliding forward. When you replace the
slide holder, do not over tighten the holding screws.
Using The Denville Scientific M2100 Microscope
Note: If your microscope has been exposed to extremes of temperature, please allow
time for all the parts to come to room temperature before turning on the power.
Excess cold can fog the lenses and may cause the bulb to fail.
1. Once you have assembled all the parts, plug the power cord into the power inlet at the
rear of the arm and plug another end of the power cord into the AC outlet
(110V/60Hz –230V/50Hz).
2. The illumination control (intensity rheostat) wheel is located on the left side of the
base. It turns the illuminator On/Off. Turn the control wheel to the brightness desired.
To turn off the illumination, simply reverse the turning until you hear a click stop.
Note: Rapid repeated changes in light intensity would dramatically shorten the life of
the quartz halogen lamp.
3. In order to speed your familiarity with controls, choose a specimen slide you are
familiar with, such as an old hematology slide or a commercially prepared slide.
Place the slide into the slide holder by pushing back on the thumb guard and placing
the slide toward the back of the holder. Allow the metal slide holder to gently hold
the slide in place.
Note: Do not allow the slide holder to “snap-back” against the slide, this could cause
the slide to chip or shatter.
4. Move the slide to the center of the stage, by turning the mechanical stage control
knobs, just below the stage on the right side. These knobs allow you to move the slide
in the X-Y axis (left-right and forward-backward).
5. Open the aperture of the iris diaphragm on the Abbe condenser (controlled by the
small black lever on the condenser).
6. Once you are comfortably seated, look into the oculars and move the eyepiece tubes
together or apart until you see only one complete circle of light. You have now
adjusted your interpupillary distance. The interpupillary distance range is 55-75mm.

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7. Focusing procedures:
7.1 Bring the 4x objective into working position. As you bring the objective into place,
will feel a “stop” (clicking) when the objective is seated properly. Use the coarse and
fine adjustment knobs to locate the image and bring the 4x objective into focus.
7.2 Move the 10x objective into place. Minor coarse adjustment may be needed yet the
fine focusing knob is needed to bring the 10x objective into focus.
7.3 Rotate to the 40x objective. Focus with fine focusing knob for the best image.
7.4 You will now be in the middle of the focus range. You may have to adjust the
aperture diaphragm (on the condenser) for the best contrast.
7.5 Immersion oil is required when 100X oil objective is used. Never allow 40X or other
dry objectives to touch immersion oil!
Note: Make sure the slide cover glass is 0.17mm or less in thickness.
8. Diopter adjustment:
If you are using a binocular, back-to-back dual binocular or trinocular microscope,
you have to adjust for the normal difference in vision between your two eyes. This is
a simple but critical adjustment! M2100 microscope has dual diopter adjustment rings
located on each eye tube of the Seidentopf head. Follow the following procedures:
Set both diopters at “0”.
Close your left eye and with your right eye open, look into the right ocular.
Adjust the fine focus to give you the best image. Do not touch the diopter on
the right eye tube.
Close your right eye and look with your left eye into the left ocular.
Rotate the adjustment (diopter) ring on the left ocular tube until you see a
clear focused field.
9. Focus Tension Control:
Focus tension has been pre-adjusted at Denville Scientific. If needed, the focus
tension can be adjusted at any time without tools. To adjust the tension of your
focusing controls, simply turn the tension control ring. This knurled ring is located on
the right side between the microscope stand and the focusing knob.
Note: Removing too much tension may cause the stage to drift down.
10. Mechanical Stage Safety Stop (Upper Limit Setting)
The safety stop is provided to help prevent objectives and slide damage. The safety
stop sets the upper limit movement of the mechanical stage. The safety stop setting
screw is located on top of stage driving block (behind the mechanical stage. Refer to
the photo with nomenclature on page 9).

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The stage upper limit is preset at Denville Scientific. If adjustment is needed, turn the
setting screw with a screwdriver to set the desired upper limit.
Note: Improper stage upper limit setting may cause high power objective unable
to focus. (The slide should almost touch the top lens of 40X objective (the top
lens of the 40X objective is retractable). when the stage is raised to its highest
limit)
You are now ready to use your M2100 microscope.
Fuse Replacement
A 1.5 Amp fuse protects the circuit board from electrical overload. The fuse case is part
of the power inlet socket located in the back of the microscope. When replacing the fuse,
always install a new one of the same size and amperage (replacement item#B3-9005).
Caution: Always unplug the microscope before changing fuse.
Follow the procedures below to replace the fuse:
a) Unplug the power cord. Turn the illuminator control wheel to “off”
b) Remove the power cord from the power inlet on the back of the
microscope.
c) Locate the fuse holder. The fuse holder is a part of the power inlet.
Microscope Stand
Back View
Power Inlet Fuse Holder
d) Use a flat head type screwdriver to take the snap-in type holder cover off.
Remove the blown fuse and replace it with the same type and rating fuse
which is:
250V 1.5A
e) Put the snap-in fuse holder back.

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Maintenance
1. Always cover your microscope with the dust cover when not in use.
2. When cleaning the lenses, use lens paper or a Q-tip dipped in lens cleaning solution.
3. Excess immersion oil should be cleaned of at once. An alcohol pad is best for
removing oil from the stage and on the other metal parts, but is not recommended for
use on the lenses.
4. Dust in the nosepiece or ocular tubes should be blown out using only filtered air.
Canned air dusters work well for this job.
5. Whenever you remove an objective, we recommend that you place the plastic cap
over the hole and put the objective back into the original plastic shipping vial until
ready to be placed back on the microscope. This will keep the objective safe from
dust and other foreign matter.
6. To keep your microscope in top condition for years, we recommend that you have the
microscope professionally serviced once a year.

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Photo & Nomenclature
High Eyepoint
Eyepieces
Diopter
Adjustment on
eyepiece tube
Revolving
Nosepiece
Objectives
Slide Holder
Mechanical Stage
Abbe Condenser
& Iris Diaphragm
Collecting Lens
(Field Lens)
Illumination base
with intensity
rheostat
Seidentopf 45º
Binocular head
Stand & Head-
Retention
Screw
Mechanical Stage
Safety Rack Setup
Adjustment
(Stage upper limit)
Mechanical Stage
Coaxial X-Y
Movement
Coaxial Coarse and
Fine Focus Knobs
Focus Tension
Control Ring
Denville Scientific M2100 Series Compound Microscope