Dionex GP40 User manual

GP40 GRADIENT PUMP
OPERATOR’S MANUAL
© Dionex Corporation 1995
Document No. 034856
Revision 03
May 1995

©1995 by Dionex Corporation
All rights reserved worldwide.
Printed in the United States of America.
This publication is protected by federal copyright law. No part of this publication
may be copied or distributed, transmitted, transcribed, stored in a retrieval system,
or transmitted into any human or computer language, in any form or by any means,
electronic, mechanical, magnetic, manual, or otherwise, or disclosed to third
parties without the express written permission of Dionex Corporation, 1228 Titan
Way, Sunnyvale, California 94088-3603 U.S.A.
DISCLAIMER OF WARRANTY AND LIMITED WARRANTY
THIS PUBLICATION IS PROVIDED “AS IS” WITHOUT WARRANTY OF
ANY KIND. DIONEX CORPORATION DOES NOT WARRANT,
GUARANTEE, OR MAKE ANY EXPRESS OR IMPLIED
REPRESENTATIONS REGARDING THE USE, OR THE RESULTS OF THE
USE, OF THIS PUBLICATION IN TERMS OF CORRECTNESS,
ACCURACY, RELIABILITY, CURRENTNESS, OR OTHERWISE.
FURTHER, DIONEX CORPORATION RESERVES THE RIGHT TO REVISE
THIS PUBLICATION AND TO MAKE CHANGES FROM TIME TO TIME
IN THE CONTENT HEREINOF WITHOUT OBLIGATION OF DIONEX
CORPORATION TO NOTIFY ANY PERSON OR ORGANIZATION OF
SUCH REVISION OR CHANGES.
TRADEMARKS
DX LANand SRSare trademarks of Dionex Corporation.
Tefzel® is a registered trademark of E.I. du Pont de Nemours & Co.
PRINTING HISTORY
Revision 01, August 1993
Revision 02, September 1993
Revision 03, May 1995

Contents
1 •Introduction
1.1 Overview . . . . . . . . . . . . . . . . . . . . . . 1-3
1.2 About This Manual . . . . . . . . . . . . . . . . 1-4
1.2.1 Typefaces . . . . . . . . . . . . . . . . . 1-5
1.2.2 Safety Messages and Notes . . . . . . . 1-5
1.2.3 Symbols . . . . . . . . . . . . . . . . . . 1-6
1.3 Related Manuals . . . . . . . . . . . . . . . . . . 1-7
2 •Description
2.1 Front Control Panel . . . . . . . . . . . . . . . . 2-4
2.1.1 Control Panel Keypad . . . . . . . . . . 2-5
2.1.2 Display Screens . . . . . . . . . . . . . 2-10
2.2 Electronics Chassis . . . . . . . . . . . . . . . . 2-11
2.3 Mechanical Chassis . . . . . . . . . . . . . . . . 2-14
2.4 Interior Components . . . . . . . . . . . . . . . . 2-14
2.4.1 Pump Eluent Manifold . . . . . . . . . . 2-15
2.4.2 Pump Heads . . . . . . . . . . . . . . . 2-16
2.4.3 Pump Mixers . . . . . . . . . . . . . . . 2-17
2.4.4 Pump Priming Block . . . . . . . . . . . 2-17
2.4.5 Pressure Transducer . . . . . . . . . . . 2-17
2.5 Vacuum Degas Pump Assembly (Optional) . . . 2-18
2.6 Eluent Reservoirs . . . . . . . . . . . . . . . . . 2-19
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2.7 Rear Panel . . . . . . . . . . . . . . . . . . . . . 2-20
2.8 Functional Description . . . . . . . . . . . . . . 2-21
2.8.1 Operating Modes . . . . . . . . . . . . . 2-23
2.8.2 Method Control . . . . . . . . . . . . . 2-24
2.8.3 Eluent Delivery . . . . . . . . . . . . . . 2-25
3 •Operation and Maintenance
3.1 Getting Ready to Run . . . . . . . . . . . . . . . 3-3
3.1.1 Degas Eluents . . . . . . . . . . . . . . 3-3
3.1.2 Filter Eluents . . . . . . . . . . . . . . . 3-4
3.1.3 Pressurize Eluent Reservoirs . . . . . . 3-4
3.1.4 Start-Up . . . . . . . . . . . . . . . . . . 3-5
3.1.5 Selecting the Pressure Limits . . . . . . 3-5
3.2 Running Under Direct Control . . . . . . . . . . 3-6
3.3 Running Under Method Control . . . . . . . . . 3-9
3.3.1 Creating a New Method . . . . . . . . . 3-10
3.3.2 Running a Method . . . . . . . . . . . . 3-12
3.3.3 Editing a Method . . . . . . . . . . . . . 3-12
3.3.4 Deleting a Method . . . . . . . . . . . . 3-14
3.3.5 Changing the Running Method . . . . . 3-14
3.3.6 Controlling the Method Clock . . . . . 3-14
3.4 Example Methods . . . . . . . . . . . . . . . . . 3-14
3.4.1 Isocratic Method Example . . . . . . . . 3-15
3.4.2 Linear Gradient Method Example . . . 3-16
3.4.3 Curved Gradient Method Example . . . 3-22
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3.4.4 Editing a Running Method Example . . 3-26
3.5 Routine Maintenance . . . . . . . . . . . . . . . 3-29
3.5.1 Daily Maintenance . . . . . . . . . . . . 3-29
3.5.2 Periodic Maintenance . . . . . . . . . . 3-31
3.6 Shutdown . . . . . . . . . . . . . . . . . . . . . . 3-31
4 •Troubleshooting
4.1 Left-Right Pump Head Pressure Fluctuations . . 4-3
4.2 Pump Will Not Start . . . . . . . . . . . . . . . . 4-6
4.3 Pump Stops . . . . . . . . . . . . . . . . . . . . 4-6
4.4 Liquid Leaks/Leak Alarm . . . . . . . . . . . . . 4-9
4.5 Noisy Pump Motor . . . . . . . . . . . . . . . . 4-11
4.6 Vacuum Degas Pump Does Not Run . . . . . . . 4-12
4.7 Vacuum Degas Pump Calibration Fails . . . . . 4-13
4.8 Inoperative Relay Control Function . . . . . . . 4-14
4.9 Poor Chromatographic Reproducibility . . . . . 4-14
5 •Service
5.1 Cleaning the Check Valves . . . . . . . . . . . . 5-3
5.2 Piston Seal Replacement . . . . . . . . . . . . . 5-6
5.3 Pump Piston Replacement . . . . . . . . . . . . 5-10
5.4 Pressure Transducer Pad and O-Ring
Replacement . . . . . . . . . . . . . . . . . . . . 5-11
5.5 Pressure Transducer Waste Valve O-Ring
Replacement . . . . . . . . . . . . . . . . . . . . 5-13
Contents
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5.6 Proportioning Valve Replacement . . . . . . . . . 5-14
5.7 Active Mixer Filter Replacement . . . . . . . . . 5-16
5.8 Changing Main Power Fuses . . . . . . . . . . . . 5-19
Contents
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A •Specifications
A.1 Electrical . . . . . . . . . . . . . . . . . . . . . . A-3
A.2 Environmental . . . . . . . . . . . . . . . . . . . A-3
A.3 Physical . . . . . . . . . . . . . . . . . . . . . . A-3
A.4 Display and Keypad . . . . . . . . . . . . . . . . A-3
A.5 Hydraulics . . . . . . . . . . . . . . . . . . . . . A-4
A.6 Gradient Control . . . . . . . . . . . . . . . . . . A-5
A.7 Vacuum Degas Pump Assembly . . . . . . . . . A-5
B •Installation
B.1 Facility Requirements . . . . . . . . . . . . . . . B-3
B.2 Installation Instructions . . . . . . . . . . . . . . B-4
B.2.1 Power Connection . . . . . . . . . . . . B-4
B.2.2 Electronic Chassis Connections . . . . . B-5
B.2.3 DX LAN Network Connection (Optional) B-7
B.2.4 Waste Lines . . . . . . . . . . . . . . . . B-9
B.2.5 Eluent Outlet Line Connection . . . . . B-10
B.2.6 Eluent Inlet Line Connections . . . . . B-10
B.2.7 Priming the Pump . . . . . . . . . . . . B-11
B.3 Automatic SRS Power Control . . . . . . . . . . B-15
B.4 Stacking Modules . . . . . . . . . . . . . . . . . B-17
B.5 Securing Modules (Optional) . . . . . . . . . . . B-18
B.5.1 Installing a Shoe . . . . . . . . . . . . . B-18
B.5.2 Installing a Tie . . . . . . . . . . . . . . B-19
Contents
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C •User Interface
C.1 Operational Screens . . . . . . . . . . . . . . . . . C-5
C.1.1 Main Screen . . . . . . . . . . . . . . . . C-5
C.1.2 Detail Screen . . . . . . . . . . . . . . . . C-7
C.1.3 Method Screen . . . . . . . . . . . . . . . C-8
C.1.4 Method Extension Screen . . . . . . . . . C-10
C.1.5 Module Setup . . . . . . . . . . . . . . . . C-11
C.1.6 Pump Options . . . . . . . . . . . . . . . C-12
C.1.7 Time Function In . . . . . . . . . . . . . . C-13
C.2 Diagnostic Screens . . . . . . . . . . . . . . . . . C-14
C.2.1 Hexadecimal Entry Fields . . . . . . . . . C-14
C.2.2 Diagnostic Menu . . . . . . . . . . . . . . C-14
C.2.3 Power-Up Screen . . . . . . . . . . . . . . C-15
C.2.4 Elapsed Time . . . . . . . . . . . . . . . . C-16
C.2.5 DSP Status . . . . . . . . . . . . . . . . . C-17
C.2.6 DX LAN Status . . . . . . . . . . . . . . C-18
C.2.7 Keyboard Test . . . . . . . . . . . . . . . C-20
C.2.8 Diagnostic Test . . . . . . . . . . . . . . . C-21
C.2.9 Pressure Statistics . . . . . . . . . . . . . C-23
C.2.10 DSP Code Version . . . . . . . . . . . . . C-24
C.3 Calibration Screens . . . . . . . . . . . . . . . . . C-25
C.3.1 Calibration Menu . . . . . . . . . . . . . C-25
C.3.2 Calibration Status . . . . . . . . . . . . . C-26
C.3.3 Leak Sensor Calibration and Status . . . C-27
C.3.4 Degas Status . . . . . . . . . . . . . . . . C-28
C.3.5 Flow Calibration . . . . . . . . . . . . . . C-29
Contents
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1 •Introduction
1.1 Overview . . . . . . . . . . . . . . . . . . . . . . . 1-3
1.2 About This Manual . . . . . . . . . . . . . . . . . 1-4
1.2.1 Typefaces . . . . . . . . . . . . . . . . . . 1-5
1.2.2 Safety Messages and Notes . . . . . . . . 1-5
1.2.3 Symbols . . . . . . . . . . . . . . . . . . . 1-6
1.3 Related Manuals . . . . . . . . . . . . . . . . . . . 1-7
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GP40 Gradient Pump
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1 •Introduction
1.1 Overview
The GP40 Gradient Pump is an integral part of a DX 500
chromatography system. It is a microprocessor-based, dual-piston,
variable-speed, gradient delivery system designed to blend and pump
mixtures of up to four different mobile phases at precisely controlled
flow rates. The pump can deliver the selected mobile phase
composition isocratically, or as a multistep linear or curved gradient.
A Digital Signal Processor (DSP) provides high speed control of
pump flow and pressure.
The GP40 can operate as a stand-alone product or with other Dionex
modules as part of a complete chromatography system. It can also
be used with non-Dionex modules that meet interface requirements
for software, TTL, or relay control.
The GP40 can be controlled locally, using the front panel keypad
and display, or from a remote host computer with a Dionex
DX LANinterface installed and PeakNet software installed on the
host computer. Limited remote control is also available from any
device capable of providing compatible TTL signals to control the
pump.
The pump’s two basic modes of control, Direct control and Method
control, enable it to operate with or without reference to time-based
events.
The GP40 is available in four versions. An optional vacuum degas
pump is available for all versions:
GP40 Gradient Pump Version With Degas Pump Without Degas
Standard bore with PEEK components P/N 044117 P/N 044165
Standard bore with stainless steel components P/N 044081 P/N 044085
Microbore with PEEK components P/N 044086 P/N 044082
Microbore with stainless steel components P/N 044083 P/N 044087
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1.2 About This Manual
This manual describes the installation, operation, and maintenance
of the GP40 Gradient Pump.
Chapter 1, Introduction, introduces the product and conventions
used in the manual, and provides safety information.
Chapter 2, Description, is a description of the physical aspects of the
pump, followed by a description of the operating features.
Chapter 3, Operation and Maintenance, discusses the operating
features and methods, and presents several examples of how to run
methods. Routine preventive maintenance requirements are included
in this chapter.
Chapter 4, Troubleshooting, lists possible causes of problems and
provides step-by-step procedures to isolate and eliminate their sources.
Chapter 5, Service, presents step-by-step instructions for service and
parts replacement routines.
Appendix A, Specifications, contains the GP40 specifications and
installation site specifications.
Appendix B, Installation, describes the installation steps necessary
to place the GP40 Gradient Pump into operation.
Appendix C, User Interface, illustrates and describes all front panel
menus and screens.
Appendix D, Pump Head Replacement, explains how to change the
pump heads from 100 µL standard bore to 25 µL microbore, or vice
versa.
Appendix E, Relay and TTL Control, describes the relay and TTL
input and output functions and provides setup examples.
GP40 Gradient Pump
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1.2.1 Typefaces
Typefaces are used in this manual as follows:
•Capitalized bold type indicates a front panel button:
Press Enter to begin running the method.
•Upper-case bold type indicates the name of a screen, the
name of a menu, or an on-screen entry:
Go to the METHOD screen.
Move the cursor to the EDIT field.
1.2.2 Safety Messages and Notes
This instrument is designed to comply with the requirements
for safety set forth in IEC 1010, Safety Requirements for
Electrical Equipment for Measurement, Control, and
Laboratory Use.
This manual contains warnings and precautionary statements
that can prevent personal injury and/or damage to the
instrument when properly followed. Safety messages appear
in bold type and are accompanied by icons.
Indicates a potential hazard which could result in serious
injury or loss of life. Any hazard of this type will be located
behind a barrier and will be accessible only by use of a
tool. Access may be required during installation,
maintenance, or service.
Indicates a potential hazard to the operator, or damage to
the instrument or other property.
Indicates that the function or process of the instrument
may be impaired. Operation does not constitute a hazard.
1 • Introduction
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Informational messages also appear throughout this manual.
These are labeled NOTE and are in bold type:
NOTE
NOTES call attention to certain information. They alert
you to an unexpected result of an action, suggest how to
optimize the performance of the instrument, etc.
1.2.3 Symbols
The symbols below appear on the pump, or on pump labels.
Alternating current
Protective conductor terminal
Power supply is on
Power supply is off
~
GP40 Gradient Pump
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1.3 Related Manuals
During installation and operation of the GP40, you may need to
refer to one or more of the following manuals (depending on your
system) for information about other modules and components
included in a DX 500 system.
The following manuals are included with their respective modules or
components:
•AS3500 Autosampler Editor Manual (Document No. 034913)
•AS40 Automated Sampler Operator’s Manual (Document
No. 034970)
•CD20 Conductivity Detector Operator’s Manual (Document
No. 034854)
•ED40 Electrochemical Detector Operator’s Manual (Document
No. 034855)
•E01 Eluent Organizer Installation Instructions (Document
No. 034582)
•Pressurizable Reservoir Installation Instructions (Document
No. 034851)
•LC10 Chromatography Organizer Operator’s Manual (Document
No. 034858)
•LC20 Chromatography Enclosure Operator’s Manual (Document
No. 034859)
•LC30 Chromatography Oven Operator’s Manual (Document
No. 034860)
The following manual is included in the GP40 Ship Kit:
•Installation of Dionex Ferrule Fittings (Document No. 034213)
1 • Introduction
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GP40 Gradient Pump
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2 •Description
2.1 Front Control Panel . . . . . . . . . . . . . . . . . 2-4
2.1.1 Control Panel Keypad . . . . . . . . . . . 2-5
2.1.2 Display Screens . . . . . . . . . . . . . . 2-10
2.2 Electronics Chassis . . . . . . . . . . . . . . . . . 2-11
LC Leak . . . . . . . . . . . . . . . . . 2-12
LC30 Communication . . . . . . . . . 2-12
LC Air Valves . . . . . . . . . . . . . . 2-13
TTL/Relay . . . . . . . . . . . . . . . . 2-13
CPU . . . . . . . . . . . . . . . . . . . 2-13
2.3 Mechanical Chassis . . . . . . . . . . . . . . . . . 2-14
2.4 Interior Components . . . . . . . . . . . . . . . . 2-14
2.4.1 Pump Eluent Manifold . . . . . . . . . . 2-15
2.4.2 Pump Heads . . . . . . . . . . . . . . . . 2-16
2.4.3 Pump Mixers . . . . . . . . . . . . . . . . 2-17
2.4.4 Pump Priming Block . . . . . . . . . . . 2-17
2.4.5 Pressure Transducer . . . . . . . . . . . . 2-17
2.5 Vacuum Degas Pump Assembly (Optional) . . . . 2-18
2.6 Eluent Reservoirs . . . . . . . . . . . . . . . . . . 2-19
2.7 Rear Panel . . . . . . . . . . . . . . . . . . . . . . 2-20
2.8 Functional Description . . . . . . . . . . . . . . . 2-21
2.8.1 Operating Modes . . . . . . . . . . . . . . 2-23
Local Mode . . . . . . . . . . . . . . . 2-23
Remote Mode . . . . . . . . . . . . . . 2-23
2.8.2 Method Control . . . . . . . . . . . . . . 2-24
2.8.3 Eluent Delivery . . . . . . . . . . . . . . 2-25
Isocratic Eluent Run . . . . . . . . . . 2-25
Gradient Eluent Run . . . . . . . . . . 2-25
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GP40 Gradient Pump
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2 •Description
The GP40 Gradient Pump consists of two single-unit DX 500
enclosures (see Figure 2-1). The upper unit houses the electronic
components, and the lower unit houses the pump heads and other
mechanical pump assemblies.
The GP40 is designed to have other single- or dual-unit modules
stacked on top of it, to a maximum of four units. See Section B.4
for the recommended stacking configuration for DX 500 systems.
Figure 2-1. GP40 Enclosure
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2.1 Front Control Panel
The control panel on the upper door of the GP40 enclosure contains
the liquid crystal display (LCD), the membrane keypad, and the
actuator for the main power switch (see Figure 2-2). The door opens
to provide access to the electronics chassis, described in Section 2.2.
Screen Contrast
Information is displayed on the LCD, also called the screen. To
adjust the screen contrast, use the knurled knob in the recess below
the keypad (see Figure 2-2).
Tilt Panel
To maximize visibility, the front control panel can be tilted to four
different positions. To tilt the panel, support the door at the left side
(to prevent it from opening) and lift firmly on the tab in the middle
of the recess below the keypad (see Figure 2-2). Push on the tab to
return the panel to its vertical position.
Power Switches
The main power switch is on the bulkhead behind the upper door
(see Figure 2-1). An actuator for the main power switch is on the
outside of the front door, at the lower left corner (see Figure 2-2).
The actuator functions only when the door is fully closed.
When the door is open, press the main power switch on the
bulkhead, instead of the actuator, to turn the module off and on.
To prevent damage to the pump circuitry and components,
always wait at least 15 seconds after powering down before
turning on the power again.
GP40 Gradient Pump
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