Hanna Instruments HI 96803 User manual

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Instruction Manual
www.hannainst.com
HI 96803
Refractometer for
Glucose
Measurements

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All rights are reserved. Reproduction in whole or in part is prohibited without the
written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode
Island, 02895, USA.
TABLE OF CONTENTS
PRELIMINARY EXAMINATION
Remove the instrument from the packing materials and examine
carefully to ensure no damage has occurred during shipping. If
any damage has occurred, notify your Dealer or closest Hanna
Customer Service Center.
Each HI 96803 instrument is supplied with:
• 9 V battery
• Instruction manual
Note:Save all packing material until you are sure that the
instrument functions correctly. A defective instrument
must be returned in its original packing.
Dear Customer,
Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using this instrument.
This manual will provide you with the necessary information for correct
use of this instrument, as well as a precise idea of its versatility.
If you need additional technical information, do not hesitate to e-mail
us at [email protected] or view our worldwide contact list at
www.hannainst.com.
PRELIMINARY EXAMINATION .................................................... 2
GENERAL DESCRIPTION ............................................................ 3
SPECIFICATIONS ........................................................................ 3
PRINCIPLE OF OPERATION ....................................................... 4
FUNCTIONAL DESCRIPTION ....................................................... 5
DISPLAY ELEMENTS .................................................................. 6
MEASUREMENT GUIDELINES ..................................................... 6
CALIBRATION PROCEDURE ....................................................... 7
MEASUREMENT PROCEDURE ..................................................... 8
MAKING A STANDARD % GLUCOSE SOLUTION......................... 9
TO CHANGE TEMPERATURE UNIT .............................................. 9
BATTERY REPLACEMENT ......................................................... 10
WARRANTY.............................................................................. 10
ERROR MESSAGES ................................................................... 11

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GENERAL DESCRIPTION
The HI 96803 Digital Glucose Refractometer is a rugged portable, water
resistant device that benefits from Hanna’s years of experience as a
manufacturer of analytical instruments.
The HI 96803 Digital Glucose Refractometer is a rugged portable, water
resistant device that benefits from Hanna’s years of experience as a
manufacturer of analytical instruments.
The HI 96803 is an optical instrument that employs the measurement of
refractive index to determine the % Glucose in aqueous solutions. The
method is both simple and quick. Samples are measured after a simple
user calibration with deionized or distilled water. Within seconds the
instrument measures the refractive index of the sample and converts it to
% by weight concentration units. The HI 96803 digital refractometer
eliminatestheuncertainityassociatedwithmechanicalrefractometersand
is easily portable for measurements on the go.
The measurement technique and temperature compensation employ
methodologyrecommendedintheICUMSAMethodsBook(Internationally
recognizedbodyforSugarAnalysis).Temperature(in°Cor°F)isdisplayed
simultaneouslywiththemeasurementonthelargedualleveldisplayalong
with icons for Low Power and other helpful message codes.
Key features include:
•Dual-levelLCD
•Automatic Temperature Compensation (ATC)
•Easy setup and storage
•Battery operation with Low Power indicator (BEPS)
•Automatically turns off after 3 minutes of non-use.
SPECIFICATIONS
Range: 0 to 85 % / 0 to 80 °C (32 to 176 °F)
Resolution: 0.1 % / 0.1 °C (0.1 °F)
Accuracy: ± 0.2 % / ± 0.3 °C (±0.5 °F)
Temperature Compensation: Automatic between 10 and 40 °C
(50 - 104 °F)
Measurement Time: Approximately 1.5 seconds
Minimum Sample Volume: 100 µL (cover prism totally)
Light Source: Yellow LED
Sample Cell: SS ring and flint glass prism
Case Material: ABS
Enclosure Rating: IP 65
Battery Type / Life: 1 X 9V / 5000 readings
Auto-Off: After 3 minutes of non-use
Dimensions: 19.2(W) x 10.2(D) x 6.7 (H)cm
Mass: 420g

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PRINCIPLE OF OPERATION
The Glucose determination is made by measuring the refractive index of
asolution. RefractiveIndex isan opticalcharacteristic ofa substanceand
the number of dissolved particles in it. Refractive Index is defined as the
ratio of the speed of light in empty space to the speed of light in the
substance. A result of this property is that light will “bend”, or change
direction,whenittravelsthroughasubstanceofdifferentrefractiveindex.
This is called refraction.
When passing from a material with a higher to lower refractive index,
there is a critical angle at which an incoming beam of light can no longer
refract, but will instead be reflected off the interface. The critical angle
can be used to easily calculate the refractive index according to the
equation:
sin (θcritical) = n2/ n1
Where n2is the refractive index of the lower-density medium; n1is the
refractive index of the higher-density medium.
In the HI 96803, light from an LED passes through a prism in contact
with the sample. An image sensor determines the critical angle at which
the light is no longer refracted through the sample. The HI 96803
automatically applies temperature compensation to the measurement
and converts the refractive index of the sample to Glucose concentration
in units of percent (by weight).

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8. Battery Cover
9. Battery Compartment
TOP VIEW
BOTTOM
FUNCTIONAL DESCRIPTION
1. Liquid Crystal Display (LCD)
2. Secondary Display
3. Primary Display
4. READ Key (User Measurement)
5. ZERO Key (User Calibration)
6. ON/OFF
7. Stainless Steel Sample Well and Prism

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DISPLAY ELEMENTS
MEASUREMENT GUIDELINES
•Handle instrument carefully. Do not drop.
•Do not immerse instrument under water.
•Donot spray waterto any part of instrumentexcept the “samplewell”
located over the prism.
•TheinstrumentisintendedtomeasureGlucosesolutions.Donotexpose
instrumentor prism tosolvents that willdamage it.Thisincludes most
organic solvents and extremely hot or cold solutions.
•Particulate matter in a sample may scratch the prism. Absorb sample
withasofttissueandrinsesamplewellwithdeionizedordistilledwater
between samples.
•Use plastic pipettes to transfer all solutions. Do not use metallic tools
such as needles, spoons or tweezers as these will scratch the prism.
•Toreducetheeffectsofevaporationorabsorptionofwaterwhentaking
readings over a period of time, the prism and sample well can be
covered with plastic wrap.
1. Battery (blinks when low battery condition detected)
2. Primary Display (displays measurement and error messages)
3. Measurement in Progress Tag
4. SETUP: Factory Calibration Tag
5. CAL: Calibration Tag
6. Measurement Unit
7. Automatic Temperature Compensation
(blinks when temperature exceeds 10-40 °C / 50-104 °F range)
8. Temperature Units
9. Secondary Display (displays temperature measurements; when
blinking, temperature has exceeded operation range: 0-80 °C / 32-
176 °F)

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CALIBRATION PROCEDURE
Calibration should be performed daily, before measurements are
made, when the battery has been replaced, between a long series of
measurements, or if environmental changes have occurred since the
last calibration.
1. Press the ON/OFF key, then release. Two instrument test screens
will be displayed briefly; all LCD segmentsfollowed by the percentage
of remaining battery life. When LCD displays dashes, the
instrument is ready.
2. Using a plastic pipette, fill the sample well with distilled or
deionized water. Make sure the prism is completely covered.
Note:If the ZERO sample is subject to intense light such as sunlight
or another strong source, cover the sample well with your hand
or other shade during the calibration.
3. Press the ZERO key. If no error messages appear, your unit is
calibrated. (For a description of ERROR MESSAGES see page 11).
Note:The 0.0 screen will remain until a sample is measured or the
power is turned off.
4. Gently absorb the ZERO water standard with a soft tissue. Use
care not to scratch the prism surface. Dry the surface completely. The
instrument is ready for sample measurement.
Note:If the instrument is turned off the calibration will not be lost.

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MEASUREMENT PROCEDURE
Verify the instrument has been calibrated before taking measurements.
1. Wipe off prism surface located at the bottom of the sample well.
Make sure the prism and sample well are completely dry.
2. Using a plastic pipette, drip sample onto the prism surface. Fill
the well completely.
Note:If the temperature of the sample differs significantly from the
temperature of the instrument, wait approximately 1 minute
to allow thermal equilibration.
3. Press the READ key. The result is displayed in units of % by weight
(w/w).
Note:The last measurement value will be displayed until the next
sample is measured or the instrument is turned off. Temperature
will be continuously updated.
Note:The ATC tag blinks and automatic temperature compensation
is disabled if the temperature exceeds the 10-40 °C / 50-104 °F
range.
4. Remove sample from the sample well by absorbing with a soft tissue.
5. Using a plastic pipette, rinse prism and sample well with distilled
or deionized water. Wipe dry. The instrument is ready for the next
sample.

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°C or °F
MAKING A STANDARD % GLUCOSE SOLUTION
To make a Glucose Solution, follow the procedure below:
•Placecontainer(suchas a glassvialordropperbottlethathasacover)
on an analytical balance.
•Tare the balance.
•TomakeanXGlucosesolutionweighoutXgramsofhighpurityGlucose
(CAS #: 50-99-7) directly into the container.
•Add distilled or deionized water to the container so the total weight
of the solution is 100g.
•Note:Solutions above 20 % may need to be vigorously stirred or
shaken and heated in a water bath. Remove solution when
glucose has dissolved. Cool completely before use.
Example with 25%:
%g Glucose g Water g Total
25 25.000 75.000 100.000
8seconds
TO CHANGE TEMPERATURE UNIT
To change the temperature measurement unit from Celsius to Fahrenheit
(or vice versa), follow this procedure.
1. Press and hold the ON/OFF key continuously for approximately
8 seconds. The LCD will display the “all segment” screen followed
by a screen with the model number on the primary display and
the version number on the secondary display. Continue pressing
the ON/OFF key.
2. While continuing to hold the ON/OFF key, press the ZERO key.
The temperature unit will change from °C to °F or vice versa.

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WARRANTY
HI 96803 is guaranteed for two years against defects in workmanship
and materials when used for their intended purpose and maintained
according to instructions.
This warranty is limited to repair or replacement free of charge.
Damage due to accidents, misuse, tampering or lack of prescribed
maintenance is not covered.
If service is required, contact the dealer from whom you purchased the
instrument. If under warranty, report the model number, date of
purchase, serial number and the nature of the problem. If the repair
is not covered by the warranty, you will be notified of the charges
incurred. If the instrument is to be returned to Hanna Instruments,
first obtain a Returned Goods Authorization number from the Technical
Service department and then send it with shipping costs prepaid.
When shipping any instrument, make sure it is properly packed for
complete protection.
To validate your warranty, fill out and return the enclosed warranty
card within 14 days from the date of purchase.
BATTERY REPLACEMENT
To replace the instrument’s battery, follow these steps:
•Make sure the instrument is off.
•Turn instrument upside down and remove the battery cover by
turning it counterclockwise.
•Extract the battery from its location.
•Replace with new 9V battery making certain to observe polarity.
•Insert the back battery cover and fasten it by turning clockwise to
engage.