Harvard Apparatus Nanoleader Series User manual

NLS110P Pressure Pump MA1 70-5000
Nano eader™Pump Series
NLS110P Pressure Pump
User’s Manua
Publication 5422-001-REV-A

EU Directives WEEE and RoHS
To Our Value Customers:
We are committe to being a goo corporate citizen. As part of that commitment,
we strive to maintain an environmentally conscious manufacturing operation. The
European Union (EU) has enacte two Directives, the first on pro uct recycling
(Waste Electrical an Electronic Equipment, WEEE) an the secon limiting the use
of certain substances (Restriction on the use of Hazar ous Substances, RoHS).
Over time, these Directives will be implemente in the national laws of each EU
Member State.
Once the final national regulations have been put into place, recycling will be offere
for our pro ucts which are within the scope of the WEEE Directive. Pro ucts falling
un er the scope of the WEEE Directive available for sale after August 13, 2005 will
be i entifie with a “wheelie bin” symbol.
Two Categories of pro ucts covere by the WEEE Directive are currently exempt
from the RoHS Directive – Category 8, me ical evices (with the exception of
implante or infecte pro ucts) an Category 9, monitoring an control instruments.
Most of our pro ucts fall into either Category 8 or 9 an are currently exempt from
the RoHS Directive. We will continue to monitor the application of the RoHS
Directive to its pro ucts an will comply with any changes as they apply.
• Do Not Dispose Product with Municipal Waste
• Special Collection/Disposal Required
WEEE/RoHS Compliance Statement

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SUBJECT PAGE NO.
General Informat on - Warranty and Repa rs ......2
Spec f cat ons ........................................................3
Introduct on ............................................................4
Hardware Overview............................................5
NLS110P - How it Works....................................6
Operat on: Gett ng Started....................................7
Do’s and Don’ts......................................................8
Troubleshoot ng ....................................................9
Software/Hardware Installat on ....................10-16
Software/Hardware Operat on ......................17-20
Tab e of Contents

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Genera Information
Serial Number
All inquires concerning our product should refer to the serial number of the unit.
Serial numbers are located on the rear of the chassis.
Calibration
All electrical apparatus is calibrated at rated voltage and frequency.While the flow
will stay calibrated, the peak will vary.
Warranty
Harvard Apparatus warranties this instrument for a period of two years from date of
purchase.At its option, Harvard Apparatus will repair or replace the unit if it is found
to be defective as to workmanship or material.
This warranty does not extend to damage resulting from misuse, neglect or abuse, nor-
mal wear and tear, or accident.
This warranty extends only to the original customer purchaser.
IN NO E ENT SHALL HAR ARD APPARATUS BE LIABLE FOR INCI-
DENTAL OR CONSEQUENTIAL DAMAGES. Some states do not allow exclu-
sion or limitation of incidental or consequential damages so the above limitation or
exclusion may not apply to you. THERE ARE NO IMPLIED WARRANTIES OF
MERCHANTABILITY, OR FITNESS FOR A PARTICULAR USE, OR OF
ANY OTHER NATURE. Some states do not allow this limitation on an implied war-
ranty, so the above limitation may not apply to you.
f a defect arises within the two-year warranty period, promptly contact Harvard
Apparatu , Inc. 84 October Hill Road, Holli ton, Ma achu ett
01746-1371 using our toll free number 1-800-272-2775. Goods will not be accepted
for return unless an RMA (returned materials authorization) number has been issued by
our customer service department.The customer is responsible for shipping charges.
Please allow a reasonable period of time for completion of repairs, replacement and
return. f the unit is replaced,the replacement unit is covered only for the remainder of
the original warranty period dating from the purchase of the original device.
This warranty gives you specific rights, and you may also have other rights which vary
from state to state.
Repair Facilitie and Part
Harvard Apparatus stocks replacement and repair parts.When ordering, please
describe parts as completely as possible, preferably using our part numbers. f practi-
cal, enclose a sample or drawing.We offer a complete reconditioning service.
CAUTION
This pump is not registered with the FDA and is not for clinical use on human
patients.

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Specificati ns
Accuracy +/- 0.05 psi
Repr ducibility +/- 0.1% over the operating range
Res luti n +/- 0.01 psi
Type Dual channel pressure pump
Pressure +15 psi to -15 psi
Fl w Rate Calibrated from experimental data
Reserv ir C lumn Variable; comes standard with 1cc
Serial P rt USB
Outputs ime (ms) and psi
P wer 24Vdc, 1.7A, 40W
V ltage Range Universal Power Supply, 100-240 VAC, 50/60 Hz
Dimensi ns 24.13 x 17.78 x 10.16 cm (9.5 x 7.0 x 4.0 in)
Weight 15.91 kg (7.23 lb)
Specifications

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Introduction
The NanoleaderTM Pump Series NLS110P Pressure Pump is a revolutionary new
approach to achieving a new level of pulseless, precise and accurate low flow
utilizing constant pressure technology.This new approach results in virtually pulse-
less flows making experimentation like bubble formation or reproducible flows in
multi-channels possible.This patented pressure monitoring system assures you the
highest quality, low flow characteristics. This pump is an easy to use computer con-
trolled dual-channel non-pulsatile pressure pump. t has two independent channels
of flow with the option of two more independent channels available on the PC
software.The NLS110P Pressure Pump comes standard with PC software and a 1 cc
reservoir column.

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Introduction
To Pressure
Sensor
Pressure Regulating
Pump Heads
Reservoir Head
and Column
Open to
Atmosphere
FRONT
Power
Switch
Power
Input Jack*
BACK
* Power Supply Input: 100-240 Vac, 50/60 HZ
Power Supply Input: 24 V c, 1.7A, 40W
Fan Side
Hardware Overview
Pump 1
Pump 2

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Introduction
AIR
NLS110P - How it Work
High Perf rmance, L w Fl w Pressure C ntr l Fl w System, Harvard Apparatus NLS110P
NLS110P - How It Works:
1. Pump generates a pressurizing
flow of air
2. Pulse of air is compressible
and dampened
3. Liquid reservoir is pressurized
4. Pressure sensor maintains a pulse
free flow based on pressure set

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Operation: Getting Started
Power Attachment
Using the provided line cord and power supply, attach the instrument to 110 VAC
or 220 VAC.
Tubing Connection
nstall tubing as shown in Figure 1. Leave one side open and attach the output
port to the provided liquid reservoir or to the input of the system to be controlled.
What’ Included
The pump comes standard with 10 feet of 1/16" OD tubing, 10 feet of 1/32" OD
tubing, fittings and ferrules.

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Do’s and Dont’s
DO: Begin with a slightly positive pressure target when connecting to an
external fluid reservoir without a filter, to ensure that liquid is not
sucked into the sensor.
DO: Press the “STOP” button in the application before closing the applica-
tion, turning off power to the pump, or unplugging the USB cable.
DON’T: Allow liquid to enter the pressure sensor. This will permanently
damage it.
DON’T: Turn on/off any power while the air reservoir is sealed.Always leave it
open to air, as the pump may spike and overload the sensor.
DON’T: Attempt to apply or measure any pressures outside of +/-15psi.
DON’T: Seal the other side of the peristaltic pump tubing.
If the system is working properly…
f connected to a sealed air reservoir, the pump should not continue to spin.
The pump should reach its target pressure within seconds.

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Troub eshooting
PROBLEM: The pump will not stop spinning when set to a non-zero
target pressure.
- There is a leak in the air pressure chamber.
- Tighten your connectors, and/or tighten the clamp arm of the peristaltic pump.
PROBLEM: The MEASURED output is abnormal, even when all tubes are
isconnecte .
- t is likely that liquid has entered the sensor and damaged it,
- There may be something else seriously wrong with the circuit board.

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Hardware/Software Insta ation
PC In tall In truction
1. nsert CD to CD/DVD drive
2. The CD content should show on the display or you can run it manually by click-
ing on my computer then navigate the CD drive usually drive D.
3. Run the application from the CD or copy the content to your PC.
4. Double Click to open the folder and then open:
NLS110P - Customer Installation >Builds >Build >My Installer >olume
>Run Setup.exe
5.The following screen will appear:
6. Select destination directory, then click next

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Hardware/Software Insta ation (Cont)
7. Choose accept, then click next
8. Choose accept, then click next

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Hardware/Software Insta ation (Cont)
9. Start nstallation by clicking next
10. All necessary files will be installed
11. Wait for the installer to finish building

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Hardware/Software Insta ation (Cont)
12. After the installer has finished building, click finish
13. After installing it will ask you to restart the PC (restart)

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Hardware/Software Insta ation (Cont)
14. After the machine restarts, shut the pump off
15. Connect the USB cable as follows: mini B terminal to the back of the pump and
the A terminal to the USB terminal on the PC
16. Connect the power adaptor to the DC input jack on the back of the pump
17.Turn on the power switch on the back of the pump
18.The PC should recognize that there is a new hardware installed
19. Select yes, this time only then click next
20. Select install from a list or a specific location then click next

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Hardware/Software Insta ation (Cont)
21. Browse to the main folder that was copied from the CD or browse the CD then
click next
22. Click continue anyway

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Hardware/Software Insta ation (Cont)
23.When its done click finish
24. Repeat the found hardware installation for the second pump-head
25. Open USBAPP.EXE, which can be found in the main folder or by navigating
through the CD
26. Click on Application .exe
27.The application window should appear
28. On the application.exe click on the dropdown box of port1 (required)
29. You should see a list of com ports, two of these com ports should have
consecutive numbers
30. Select the lower numbered com port (between the two consecutive ports)
31. Click on the dropdown box of port2 (optional)
32. Select the higher numbered com port (between the two consecutive ports)
33. Change # of attached channels to 2 (Only if you are using both pump heads)

1. Click the RUN arrow on the top left of the screen (underneath “Edit”)
2.The graph on the right should show lines on the 0 amplitude after a few seconds
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Software/Hardware Operation

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Software/Hardware Operation (Cont)
3. Select Set Pressure tab ( f calibration is needed, skip to step 5 first)
4. On the PS target choose the desired pressure for each channel
5. Calibration: for each pump head choose the appropriate calibration (ex.
Calibration #1) tab and enter in the calibration values provided. The calibration
values can be found on the back of the pump directly behind the corresponding
pump head*. Each pump head may be recalibrated with the use of a manometer
by following the “Calibration nstructions” document on the CD.
* See label on rear panel for calibration values for each pressure hea .