HARVARD CMA 7 User manual
CMA 7 55 kDa Microdialysis Probe User’s Manual
TECHNICAL INFORMATION
Membrane
Material Polyethersulfone (PES)
Molecular Cut-O 55,000 Daltons
Outer Diameter 0.26mm
Length 1 and 2 mm
Probe Shaft
Material Stainless-steel
Diameter 0.40 mm
Length 7 mm
Internal Volume
Inlet Volume 0.06 µL
Outlet Volume 0.3 µL
200 mm Inlet Tubing (blue) 3.5 µL
200 mm Outlet Tubing (transparent) 3.5 µL
Instructions for CMA 7 55 kDa Microdialysis Probe
1. Fill a microsyringe with perfusion uid and mount it in the CMA Syringe Pump. The Perfusion Fluid must be clean, at
room temperature and preferably degassed.
2. Run the pump to make sure that liquid leaves the tip of the syringe cannula.
3. Connect the desired length of tubing to the inlet and outlet of the probe. Short cannula = inlet, long cannula = outlet.
Tubing Adaptors and FEP Tubing should be used for all connections. To facilitate the handling of Tubing Adaptors,
they should be pre-soaked in ethanol for a minimum of 10 minutes.
4. Mount the microdialysis probe CMA 7 & 8 Probe Clip on the CMA/130 in vivo Stand. Put the microdialysis probe in a
vial filled with perfusion fluid.
5. Connect the inlet tubing of the microdialysis probe to the syringe cannula, by sliding the Tubing Adapter over the cannula.
Wait for 10 min. The Tubing Adapter must be dry before ushing.
6. Flush the probe with 8-10 µL/min in the Perfusion Fluid for 4-5 min to wash out air. While ushing, “tap” with a scissor on
the probe clip (not the probe) to remove air bubbles. The vibrations from the probe clip will in most cases remove the air
bubble. If possible, check for air bubbles under a stereomicroscope. If the air bubble is not gone, the ushing and “tap-
ping” must be repeated. The membrane is light blue when wetted, air bubbles occur as whiter spots. When ushing the
membrane it may appear to be “sweating” which is due to ultraltration of uid through the membrane.
7. Set the pump to the required perfusion ow (usually 1-5 µL/min) and check for leaks.
The microdialysis probe is now ready for use.
8. When changing sample vials, remember to consider the internal volume in the system (see TECHNICAL
INFORMATION). This causes a delay that must be calculated when using low perfusion rates and short sampling times.
9. After the experiment, put the microdialysis probe in a vial lled with deionized water. Perfuse with deionized water to
prevent salt crystal formation. The probe can be stored in deionized water.
WARRANTY
The probes manufactured by CMA Microdialysis are warranted to be free from defects in material and workmanship for
a period of two years from the manufacturing date if stored in the original package.
Claims should be forwarded without delay to CMA Microdialysis or to your local distributor.
CMA Microdialysis AB
Head Oce, Sweden
Torshamnsgatan 30A, SE-164 40 Kista, Sweden
Tel: +46 8 470 10 00
Harvard Apparatus
84 October Hill Road
Holliston, MA 01746 USA
Phone Orders: 800-232-2380 • Fax: 508-429-5732
The CMA 7 Microdialysis Probe is not intended for use in humans. It is only suitable for laboratory research in animals.
CMA Microdialysis only guarantees single usage of CMA 7 Microdialysis Probes.
ORDER INFORMATION Ref No.
CMA 8012411
CMA 8012412
CMA 7 55kDa Microdialysis Probe, 1 mm, 3/pkg*
CMA 7 55kDa Microdialysis Probe, 2 mm, 3/pkg*
*Metal Free and β –Irradiated Probes are available as Custom Probes
CMA 7 Guide Cannula, 3/pkg CMA P000137
CMA 7 Guide Cannula, 30/pkg CMA P000138
CMA 7 Guide Metal Free, 3/pkg CMA 8010773
CMA 7 Guide β–Irradiated, 3/pkg CMA 8010683
CMA 7 Guide β–Irradiated, 30/pkg CMA 8010684
Tubing Adapter, 10/pkg CMA 3409500
FEP Tubing 1 m, 1/pkg CMA 3409501
FEP Tubing 1 m, 10/pkg CMA 8409501
Tubing Connector, 3/pkg CMA P000113
CMA 7 & 8 Probe Clip CMA P000136
OPTIONAL ACCESSORIES Ref No.
CMA 4004 Syringe Pump CMA 400400
CMA 402 Microdialysis Pump with
Accessory Kit
CMA 8003100
CMA 402 Microdialysis Pump CMA 8003110
CMA 110 Liquid Switch CMA 8308200
CMA 130 In Vitro Stand with CMA 7 clips CMA 8309104
CMA 142 Microfraction Collector 230V CMA 8381142
CMA 142 Microfraction Collector 115V CMA 8381143
CMA 470 Refrigerated Fraction Collector CMA 8002770
Microsyringes 1 mL CMA 8309020
Microsyringes 2.5 mL CMA 8309021
For other probes and microdialysis accessories please call
your local CMA Microdialysis dealer.
8012410A
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