Invitrogen PowerEase 500 User manual

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PowerEase®500
Power Supply
Version D
032202
IM-8634
PowerEase®500 Power Supply
A programmable power supply for electrophoresis
Catalog nos. EI8600, EI8700, EI8675
www.invitrogen.com
[email protected]
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Warning
Federal
Communications
Commission
Advisory
This equipment has been tested and found to comply with the limits for a Class
A digital device, pursuant to part 15 of the FCC rules. These limits are designed
to provide reasonable protection against harmful interference when the
equipment is operated in a commercial environment. This equipment
generates, uses, and can radiate radio frequency energy and, if not installed and
used in accordance with the instruction manual, may cause harmful
interference to radio communications. Operation of this equipment in a
residential area is likely to cause harmful interference in which case the user
will be required to correct the interference at their expense.
Changes or modifications not expressly approved by the party responsible for
compliance could void the user’s authority to operate the equipment.
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Table of Contents
Warning.................................................................................................................................................................iii
Table of Contents...................................................................................................................................................v
Kit Contents ..........................................................................................................................................................vi
Description...........................................................................................................................................................vii
Safety Information................................................................................................................................................xi
Consignes De Securite ........................................................................................................................................xii
Sicherheits Anweisungen..................................................................................................................................xiii
Accessory Products............................................................................................................................................xiv
Introduction....................................................................................................................1
Overview................................................................................................................................................................ 1
Methods..........................................................................................................................3
Getting Started....................................................................................................................................................... 3
Operational Modes................................................................................................................................................ 4
Using PowerEase®500 .......................................................................................................................................... 5
Printing ................................................................................................................................................................. 12
Custom Methods ................................................................................................................................................. 13
Troubleshooting .................................................................................................................................................. 16
Appendix ......................................................................................................................17
Warranty............................................................................................................................................................... 17
Run Conditions for Gels..................................................................................................................................... 18
Repair and Maintenance of PowerEase®500................................................................................................... 19
Theory of Operation............................................................................................................................................ 20
Technical Service ................................................................................................................................................. 21
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Kit Contents
Types of Kits This manual is supplied with the following kits:
Kit Catalog no.
PowerEase®500 Power Supply (100/120 Vac 50/60 Hz) EI8600
PowerEase®500 Power Supply (220/240 Vac 50/60 Hz) EI8700
PowerEase®500 Pre-Cast Gel System
includes PowerEase®500, Temperature Monitoring Probe, XCell
SureLock™Mini-Cell and XCell II™Blot Module
EI8675
Kit Components PowerEase®500 Power Supply contains:
PowerEase®500 Power Supply 1 each
Instruction Manual 1 each
Extra Fuses 2 each
Power Cord (U.S., Canada, Europe, Taiwan and Japan) 1 each
Warranty Card 1 each
Temperature Probe (Optional) 1 each
See next page for specifications and detailed description of PowerEase®500
Power Supply.
To ensure safe, reliable operation, always operate the PowerEase®500 Power
Supply in accordance with the manufacturer’s instructions. Always wear
protective gloves and safety glasses when working in a laboratory environment.
See safety information on pages xi-xiii.
Warranty information is provided on page 17.
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Description
Specifications Input Power
100/120 VAC 90-132V, 50/60 Hz, 100 watts at maximum load
Fuse One 1A/250V/AGC, fast blow fuse
220/240 VAC 198-264VAC, 50/60 Hz, 260 mA at maximum load
Fuses Two F1A/250V/5x20 mm fast blow fuses
Output Range 1-500 V (minimum step size 1 V)
1-500 mA (minimum step size 1 mA)
0.1-50 W (minimum step size 0.1 W)
Automatic crossover on reaching set limits.
Accuracy Voltage: ± 2% or ± 2 volts
Current: ± 2% or ± 2 mA
Wattage ± 2% or ± 0.2 watt
Drift < 1% in 8 hours after 30 minute warm-up
with constant supply voltage
Circuit Protection Open Circuit; Short Circuit; Thermal Protection
Over Voltage, Current, Wattage
Safety Load Detection
Thermal Shutoff (with optional temperature probe)
Isolated Floating Ground
Memory Control Non-volatile, up to 24 hours
Custom Methods Stored to RAM
Computer Interface RS232C
Display LCD, view area 114 x 64 mm
240 x 128 dots
Temperature Ambient 4-30°C
Dimensions 16.5 cm (w) x 18 cm (h) x 20.5 cm (d)
Weight 2.9 kg (6.4 lbs)
Environmental
Conditions ≤100% RH, 75 KPa-106 KPa
Continued on next page
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Description, Continued
Front View of
PowerEase®500
+
TEMP
P
o
w
e
r
E
a
s
e
5
0
0
SELECT
S
T
O
P
S
T
A
R
T
POWER HIGH VOLTAGE
1
7
4
3
5
2
6
8
1. LCD Screen
2. Power Output Jacks, Positive (Red)
3. Power Output Jacks, Negative (Black)
4. Temperature Probe Jack
5. Functional Keys (Power, Start, Stop)
6. Directional Keys (Up, Down, Left, Right)
7. High Voltage LED
8. Power LED
Continued on next page
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Description, Continued
Rear View of
PowerEase®500
PRINTER
RS232
an
RS232C
Communi-
cation
Port
Printer
Port
Power Entry Module
with On/Off
main power switch
Continued on next page
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Description, Continued
Key Pad
Power button secondary On/Off switch for the high voltage components, fan, and LCD
screen.
Start button used to start a selected method program.
Stop button used to end the selected method and to backup to a previous screen.
SELECT
Select button used to select a menu choice and to move forward to the next applicable
screen.
Left directional button for moving left around the menu.
Right directional button for moving right around the menu.
Up directional button for moving up around the menu and to adjust numerical and
character values (higher).
Down direction button for moving down around the menu and to adjust numerical and
character values (lower).
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Safety Information
Avoiding
Electrical Shock
The PowerEase®500 Power Supply produces high voltage outputs which are
electrically isolated from earth ground to reduce the risk of electrical shock to
the user. Observe the following guidelines to ensure safe operation of the unit.
The PowerEase®500 Power Supply has been designed for use with
electrophoresis cells with shielded banana plugs thus minimizing potential
shock hazard to the user. Invitrogen does not recommend use of other
unshielded banana plugs.
To avoid electrical shock:
1. NEVER connect or disconnect wire leads from the power jacks when the
amber high voltage indicator light is on.
2. WAIT at least 5 seconds after stopping a run before handling output leads
or connected apparatus.
3. ALWAYS make sure hands and work area is clean and dry before making
any connections or operating the power supply.
4. ONLY connect the power supply to a properly grounded AC outlet.
Avoiding Damage
to the Instrument
1. For proper ventilation, leave at least 10 cm of space behind the instrument,
and at least 5 cm of space on each side.
2. Do not operate the power supply in high humidity environments (> 95%), or
where condensation can occur.
3. To avoid condensation after operating the power supply in a cold room, wrap
the unit in a plastic bag and allow at least 2 hours for the unit to equilibrate to
room temperature before removing the bag and operating the unit.
Symbols The symbols used on the PowerEase®500 Power Supply are explained below.
Used on the PowerEase®500 Power Supply to indicate an area where a
potential shock hazard may exist.
Used on the PowerEase®500 Power Supply to indicate a warning. Consult the
manual to avoid possible personal injury or instrument damage.
Used on the PowerEase®500 keypad to indicate the secondary Power button.
Used on the PowerEase®500 keypad to indicate the Start method button.
Used on the PowerEase®500 keypad to indicate the Stop method button.
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Consignes De Securite
Consignes De
Securite
Le PowerEase®500 génère des courants de haut voltage; la construction du
système est faite suivant toutes précautions pour isoler l’utilisateur et éviter
tout choc électrique. Toutefois, il est impératif d’observer les consignes
suivantes pour éviter tout tisque lors de l’utilisation de l’appareil.
PowerEase®500 est destiné à être utilisé avec des curves d’electrophorèse
équipées avec des fiches banane protégées pour minimiser tout risque de choc
électrique. L’utilisation d’autres types d’équipments n’est pas recommandée
par Invitrogen et se fait aux risques et périls de l’utilisateur.
Pour éviter Tout
Choc électrique
1. Ne jamais connecter ou déconnecter les cables des fiches quand l’indicateur
haut voltage est allumé..
2. Attendre un minimum de 5 secondes après avoir arrêté le courant avant de
manipuler le système.
3. Vérifier que vos mains sont séches et propres avant d’effectuer toute
connection.
4. Utiliser des prises de courant munies d’une connection à la terre.
Pour éviter
d’endommager
l’appareil:
1. Assurer une ventilation correcte, en laissant un minimum d’espace derrière
(10 cm) et sur les côtés (5 cm) de l’appareil.
2. N’utilisez pas le générateur dans un environement humide (> 95%) ou avec
des risques de condensation.
3. Pour éviter la condensation lors de l’utilisation de l’appareil en chambre
froide, enveloppez-le dans une poche plastique et laisser l’appareil
s’équilbrer à la température ambiante avant de retirer le sac et de l’utiliser.
Signification des pictogrammes
Sur le PowerEase®500, signale les zones où un risque de choc électrique peut
exister.
Sur le PowerEase®500, signale un risque potentiel pour l’utilisateur ou pour
l’equipement. Veuillez consulter le mode d’emploi.
Sue le panneau du PowerEase®500; bouton d’alimentation secondaire.
Sur le panneau du PowerEase®500; permet de démarrer une méthode.
Sur le panneau du PowerEase®500; bouton d’interruption.
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Sicherheits Anweisungen
Sicherheits
Anweisungen
Das PowerEase®500 Stromversorgungsgerät produziert Gleichstrom-
Hochspannung, die elektrisch getrennt ist vom Erdleiter, um das Risiko des
Benutzers für einen Stromschlag zu minimieren. Trotzdem sollten folgende
Sicherheitsrichtlinien beachtet werden, damit ein einwandfreier Betrieb des
Gerätes gewährleistet ist.
Das PowerEase®500 ist entwickelt worden für den Betrieb von
Elektrophoresezellen mit isolierten Bananen-Schutzkontaktsteckern. Somit ist
eine Gefährdung durch Stromschlag bei sachgerechter Benutzung ausge
schlossen. Die Verwendung von anderen, ungeschützten Bananensteckern
geschieht auf Risiko des Benutzers und Invitrogen rät ausdrücklich davon ab.
So verhindern Sie
eine Gefährdung
durch
Stromschlag
1. Stecken (oder ziehen) Sie niemals Kabel in die Stromausgangsbuchsen am
Gerät, wenn das gelbe Licht (Hochspannungs-Indikator) am
Bedienungsfeld leuchtet.
2. Warten Sie mindestens 5 Sekunden nach Beendigung und Ausschalten
eines Laufs, bevor Sie die Anschlubkabel der Zelle herausziehen.
3. Achten Sie darauf, dab die Arbeitsplatzumgebung der Zelle sauber und
rocken ist. Sie sollten niemals mit nassen Händen Gerät, Kabel oder
Steckverbindungen anfassen.
4. Schlieben Sie das PowerEase®500 nur an eine einwandfrei geerdete 220-
230V Strombuchse an.
Um Beschädigung
des Geräts zu
vermeiden
1. Achten Sie auf wenigstens 10 cm Platz hinter und 5 cm neben dem Gerät,
um seine eingebaute Kühlventilation nicht zu beeinträchtigen.
2. Verwenden Sie das Gerät nicht in Räumen mit hoher Luftfeuchtigkeit
(>95%) oder wo es Wasserdampfkondensation ausgesetzt ist.
3. Ist das Gerät in einem Kühlraum verwendet worden, und soll es nach der
Benutzung wieder unter Normaltemperaur arbeiten, ist es vor der
Umsetzung in eine Kunststoffhülle zu packen. Es soll mindestens 2 Stunden
darin auf die neue Umgebungstemperatur angepasst werden, bevor die
Hülle entfernt und das Gerät wieder in Betrieb genommen wird.
Symbolerklärung
Weist auf eine Zone hin, wo Gefährdung durch Stromschlag besteht.
Lesen Sie die Bedienungsanleitung, um Verletzungen oder Beschädigung des
Gerätes zu vermeiden.
1st der 2. Bertiebsschalter auf dem PowerEase®500—Bedienungsfeld.
Start—Knopf auf dem Bedienungsfeld für eine programmierte Methode.
Stop—Knopf auf den Bedienungsfeld für einen Elektophorese—Lauf.
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Accessory Products
Additional
Products
Additional products that can be used with the PowerEase®500 Power Supply
or replacement parts for PowerEase®500 Power Supply are available separately
from Invitrogen. For more information, contact Technical Service (see page 21)
or visit our Web site at www.invitrogen.com.
Product Quantity Catalog no
Temperature Monitoring Probe 1 EI8644
RS232 Cable and Adaptor 1 EI8663
Fuses 1A/250V/3AGL Fast Blow (100-120VAC units) 2 EI8670
Fuses F1A/250V/5x20mm Fast Blow (220-240V units) 4 EI8671
XCell SureLock™Mini-Cell 1 unit EI0001
XCell II™Blot Module 1 unit EI9051
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Introduction
Overview
Introduction The PowerEase®500 Power Supply is a microprocessor-controlled power supply
for electrophoresis of pre-cast and hand-poured mini-gels. The power supply is
designed to simplify electrophoresis by combining high performance and
programming flexibility with ease of use when running mini-gel and blotting
applications.
The PowerEase®500 Power Supply is designed around the single gel concept:
you need to think only about the electrical parameters and limits for a single gel.
This manual describes the setup and operation of PowerEase®500 Power Supply
including important information on safety and maintaining the unit.
Features of
PowerEase®500
The important features of PowerEase®500 Power Supply are listed below:
• Large LCD display allows electrical parameters to be displayed in either
graphical or numerical formats
• Eight pre-programmed methods for running and blotting Novex®pre-cast
mini-gels
• Four custom methods for running your custom applications
• Simple three step operating procedure to start running or blotting Novex®
pre-cast gels
• Microprocessor control provides highly accurate electrical outputs resulting
in exceptional blotting capability
• Parallel port for printing experimental results to most printers
• Optional Temperature Probe to continuously monitor the buffer temperature
and shut the unit down in the event of overheating
• Two sets of output jacks allows electrophoresis of multiple mini-cell units in
constant voltage, current, or watts
Continued on next page
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Overview, Continued
Flowchart The flowchart below describes the various screens displayed on the PowerEase®
500 Power Supply and the keypad buttons (Start, Stop or Select) used to
navigate through the screens.
Start/Select
Start
Select
Power ON
Start-Up Screen
Gel Method Screen
Gel Quantity Screen
Method Run Large Display
Stop
Select
Method Edit Screen Method Run Large Display Pre ious Screen
StopStart
Pre ious Screen
Select, once Select, twice
Select Method Run/Edit Screen
Method Run Graphic Display
Select, onceSelect, twice
Method Run Large Display
Method Run Graphic Display
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Methods
Getting Started
Installing
PowerEase®500
1. Place the PowerEase®500 Power Supply on a level bench. Keep the area
around the power supply clear to ensure proper ventilation of the unit.
2. Position the unit properly such that the On-Off switch and the power cord
attachment module located on the rear of the unit are easily accessible.
3. Check the label located near the power entry module to ensure that the unit
is of the proper local voltage. Attach the power cord to the power entry
module. Use only properly grounded AC outlets.
Installing an
Optional Printer
The PowerEase®500 Power Supply has a DB25 parallel connector located on the
rear of the unit. This allows the PowerEase®500 Power Supply to print a report
of your electrophoresis results in ASCII format to most commonly available
printers.
Use the following steps to install a printer:
1. Using a parallel printer cable, connect the male DB25 end of the printer cable
into the port marked “Printer” located on the rear of the PowerEase®500
Power Supply.
2. Connect the opposite end of the cable into the appropriate connector on the
printer. Refer the user manual of your printer for instructions on connecting
the cable to the printer.
Installing an
Optional
Temperature
Probe
If you have ordered the Temperature Monitoring Probe, follow the instructions
below to install the probe:
1. Unpack the Temperature Monitoring Probe and inspect for any possible
damage to the wire or connector which may have occurred during shipping.
2. Plug the connector end of the Temperature Monitoring Probe into the port
located at the front lower right corner of the PowerEase®500 Power Supply
marked “TEMP”.
3. Place the opposite end of the Temperature Monitoring Probe with the blue-
colored bead into an electrophoresis cell buffer chamber to display
temperature on the LCD screen of the PowerEase®500 Power Supply. You
can bent the wire, but avoid crimping or pinching the wire. Temperature will
be displayed at all times even when the PowerEase®500 Power Supply is
stopped.
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Operational Modes
Introduction The PowerEase®500 Power Supply is capable of operating at limiting voltage,
limiting current, and limiting power. We recommend operating the PowerEase®
500 Power Supply at limiting voltage for most applications. See below for more
details.
Voltage Limiting
The recommended setting for operating the PowerEase®500 Power Supply is
voltage limited. For most electrophoresis methods, resistance increases
throughout the run. Using voltage limiting provides the following advantages:
• Current and watts decrease throughout the run, providing a natural safety
margin.
• The same voltage setting can be used regardless of the number or thickness
of gels being electrophoresed.
Current Limiting Discontinuous buffer systems and, to a lesser extent continuous systems,
increase resistance during the run. If you use the current limiting setting on the
PowerEase®500 Power Supply, the voltage will increase as resistance increases
to satisfy Ohm’s law (V=IR, see page 20). If no voltage limit is set and a local
fault condition occurs, such as a poor connection, very high local resistance
may cause the voltage to increase to a maximum of the power supply. This will
lead to local overheating and damage to the electrophoresis cell or create unsafe
conditions.
When running under constant current conditions, set a voltage limit on the
power supply at or slightly above the maximum expected voltage.
Wattage Limiting If power is constant, voltage will increase and current will decrease during a
run, but the total amount of heat generated by the system will remain constant
throughout the run. However, locally high resistance can cause a high
proportion of the total heat to be generated over a small distance. This can
damage the electrophoresis cell and/or gel(s).
If operating at wattage limiting, set the voltage limiting to slightly above the
maximum expected for the run.
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Using PowerEase®500
Introduction You can operate the PowerEase®500 Power Supply immediately after
installation using three simple steps (see below). For programming custom
settings, see page 13.
Starting
PowerEase®500
1. Plug the power cord of the PowerEase®500 Power Supply into an available
electrical outlet rated at the appropriate electrical values for the power
supply.
2. Turn on the main power switch located on the rear of the PowerEase®500
Power Supply (see page ix for rear view of PowerEase®500 Power Supply).
Always leave this switch in the ON position to keep the back-up battery fully
charged.
3. Press the Power button located on the keypad (see page x for a figure of the
keypad). This Power button is a secondary power button and does not
actually shut the instrument down, but shuts down the high voltage, fan,
and LCD screen. When powered back up, the LCD screen will revert to the
same screen as when the secondary power was shut off.
4. A Start-Up screen will appear (see next page).
To avoid condensation, the main power switch located on the rear of the unit
should be left on between use during operation in a cold room or in high
humidity environments.
Operating
PowerEase®500
A simple operating procedure of the PowerEase®500 Power Supply is provided
below. For more details on the different screens and custom methods, see pages
5-13.
1. Turn on the PowerEase®500 Power Supply.
2. Press Select once the Start-Up screen appears.
3. Select your gel type from the Gel Method screen.
4. Choose the number of gels to be electrophoresed from the Gel Quantity
screen.
5. Press the Start button to begin electrophoresis.
Note: To reset the PowerEase®500 Power Supply, press the Stop button.
The maximum number of Novex®mini-gels that can be electrophoresed at the
same time using PowerEase®500 Power Supply and the XCell SureLock™Mini-
Cell is listed below:
Gel Type Number of Gels
Novex®Tris-Glycine Gels 10 gels
NuPAGE®Novex Tris-Acetate Gel 6 gels
NuPAGE®Novex Bis-Tris Gels 2 gels
Novex®Tricine Gels 4 gels
Novex®TBE-Urea gels 10 gels
Continued on next page
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