Invitrogen PowerEase PSHV00 User manual

For Research Use Only. Not for use in diagnostic procedures.
PowerEase™Touch HV Power Supply
USER GUIDE
Catalog NumbersPSHV00 (115 VAC), PSHV01 (230 VAC), PSHV02 (230 VAC Australia),
PSHV03 (230 VACChina)
Publication NumberMAN0028692
Revision A.0

Shanghai Life Technologies Biotechnology Co., LTD | No.7 Rong Chang East Street, Long Sheng Industry Park | Building 203 BDA,100176,
P.R.China
For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.
Revision history:MAN0028692 A.0 (English)
Revision Date Description
A.0 3 March 2023 New document for PowerEase™Touch HV Power Supply.
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE
LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR
ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these
products, you accept the terms and conditions of all applicable Limited Use Label Licenses.
TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
©2023 Thermo Fisher Scientific Inc. All rights reserved.

Contents
■CHAPTER1Productinformation .................................................. 5
Productdescription ............................................................. 5
Product contents ................................................................ 7
■CHAPTER2Methods ............................................................... 9
Proceduralguidelines ............................................................ 9
Install the powersupply ......................................................... 10
HV adapters ................................................................... 10
Programingoptions ............................................................ 11
Menunavigation ............................................................... 11
Home screen .................................................................. 11
Set up arun ................................................................... 12
Constant current ........................................................... 13
Constantpower ........................................................... 14
VoltageRamp ............................................................. 14
Custommethods .............................................................. 14
Select a pre-programedmethod ................................................. 15
IPG stripfocusing .............................................................. 17
Managemethods .............................................................. 17
Save amethod ............................................................ 17
Edit amethod ............................................................. 18
Delete amethod ........................................................... 19
Change the name of a savedmethod ........................................ 19
Add a step ................................................................ 20
Delete a step .............................................................. 20
Save amethod ............................................................ 20
Instrument display during arun .................................................. 21
Run screen features ........................................................ 21
Method Settings screen features ............................................ 22
Instrument display after run completion .......................................... 23
Settings ....................................................................... 24
Settings screen features .................................................... 25
PowerEase™Touch HV Power Supply User Guide 3

■APPENDIXATroubleshooting .................................................... 29
■APPENDIXBRepair and maintenance .......................................... 32
Encountering Problems ......................................................... 32
Replace thefuse ............................................................... 32
■APPENDIXCElectrical parameters for electrophoresis ........................ 34
Ohm’s Law and Joule’sLaw ..................................................... 34
Resistance .................................................................... 34
Voltage ....................................................................... 34
Current ....................................................................... 34
Power ........................................................................ 34
■APPENDIXDGeneralsafety ...................................................... 35
Instrument safety .............................................................. 35
Avoid electricalshock ...................................................... 35
Avoid damage to theinstrument ............................................. 35
Dischargefunction ......................................................... 36
Explanation of symbols andwarnings ............................................ 36
Symbols andwarnings ..................................................... 36
Product regulatory compliance .................................................. 37
Federal Communications Commission Advisory ............................... 37
Environmental designcompliance ........................................... 37
■APPENDIXEProductspecifications ............................................. 38
Productspecifications .......................................................... 38
■APPENDIXFDocumentation and support ...................................... 40
Customer and technical support ................................................. 40
Limited product warranty ........................................................ 40
Contents
4PowerEase™Touch HV Power Supply User Guide

Product information
Product description
The Invitrogen™PowerEase™Touch HV Power Supply is a high voltage power supply that uses a
touchscreen interface. This power supply is ideal for DNA/RNA electrophoresis, SDS-PAGE, IEF, IPG,
and native PAGE.
The power supply unit has the following features:
•Touchscreen LCD display and user interface with clear menu prompts for easy use by hand or
stylus
•Maximum output of 3500 V, 500 mA, and 250 W (for complete specifications see “Product
specifications” on page38)
•Four sets of output terminals allow running of multiple electrophoresis units
•Pop-up feet allow change to screen viewing angle
•Sturdy polyurethane feet and stackable housing design allow stacking of power supplies
•Running modes include Constant Voltage, Constant Power, Constant Current, or Voltage Ramp
•Programming capabilities for limiting voltage (V), current (mA) and power (W)
•Ability to power o No Load Detection (NLD) for low current runs (see “No Load Detection” on
page27)
•Method programming flexibility (up to 100 methods, 20 steps per method, 999 minutes per step)
•Pause button for editing method parameters during the run
•User-adjustable device settings such as beep volume, screen brightness and sleep mode duration
•Automatic safety features such as No Load, Over Temperature, Over Voltage, Over Current, Load
Change, Ground Leak detection, and Discharge function
This manual describes the setup and operation of the PowerEase™Touch HV Power Supply and
includes important information about safety and maintenance of the unit.
1
PowerEase™Touch HV Power Supply User Guide 5

Figure1Front view of PowerEase™Touch HV Power Supply.
Figure2Rear view of PowerEase™Touch HV Power Supply.
Chapter1Product information
Product description
1
6PowerEase™Touch HV Power Supply User Guide

Figure3USB port dust cover (Rear view of PowerEase™Touch HV Power Supply).
The USB port is shipped with a plastic dust cover for protection during shipment. This cover must be removed before port
use. Remove by gently prying o with a small flat blade screwdriver.
Product contents
On receipt, examine the unit carefully for any damage incurred during transit. Any damage claims
must be filed with the carrier, and keep the supplied box for inspection. The warranty does not cover
in-transit damage.
IMPORTANT! Some power cords appear similar; use the cord approved for the country/region.
·North America – Type B power cord
·Japan – JIS 8303 power cord
·China – GB 15934-2008 power cord
·Australia/New Zealand – Type I power cord
Table1Cat. No. PSHV00 (115 VAC)
Component Quantity Regulatory mark(s) on electrical plug
PowerEase™Touch HV Power Supply 1 each —
PowerEase™Touch HV Adapters 4 sets —
Extra Fuse 1 each —
Type B Power Cord 1 each CSA
JIS 8303 Power Cord 1 each PSE
USB Cover 1 each —
Quick Reference Card 1 each —
Certificate of Performance 1 each —
Chapter1Product information
Product contents 1
PowerEase™Touch HV Power Supply User Guide 7

Table2Cat. No. PSHV01 (230 VAC)
Component Quantity Regulatory mark(s) on electrical plug
PowerEase™Touch HV Power Supply 1 each —
PowerEase™Touch HV Adapters 4 sets —
Extra Fuse 1 each —
Type G Power Cord 1 each ASTA
Type CEE 7/7 Power Cord 1 each CE
USB Cover 1 each —
Quick Reference Card 1 each —
Certificate of Performance 1 each —
Table3Cat. No. PSHV02 (230 VAC Australia)
Component Quantity Regulatory mark(s) on electrical plug
PowerEase™Touch HV Power Supply 1 each —
PowerEase™Touch HV Adapters 4 sets —
Extra Fuse 1 each —
Type I Power Cord 1 each NSW 18392
USB Cover 1 each —
Quick Reference Card 1 each —
Certificate of Performance 1 each —
Table4Cat. No. PSHV03 (230 VAC China)
Component Quantity Regulatory mark(s) on electrical plug
PowerEase™Touch HV Power Supply 1 each —
PowerEase™Touch HV Adapters 4 sets —
Extra Fuse 1 each —
Type GB 15934-2008 Power Cord 1 each CCC
USB Cover 1 each —
Quick Reference Card 1 each —
Certificate of Performance 1 each —
Chapter1Product information
Product contents
1
8PowerEase™Touch HV Power Supply User Guide

Methods
Procedural guidelines
We recommend that you carefully review these guidelines before operating the instrument.
•To ensure safe, reliable operation, always operate the PowerEase™Touch HV Power Supply in
accordance with the manufacturer’s instructions.
•Avoid exposing the power supply to liquids as these can damage the unit and void the warranty.
•IMPORTANT! Do not use the PowerEase™Touch HV Power Supply at its maximum electrical load
limits. Variations in buer conditions can result in exceeding the power supply’s maximum voltage,
current, or power output capacity and produce undesirable variations in performance.
•When using this or any electrophoresis product, we recommend that you adhere to the protocols
given in the electrophoresis product manuals.
For best results:
•We recommend using the Constant Voltage mode for protein gels, nucleic acid gels, or blots.
For the PowerEase™Touch HV Power Supply, a Voltage Ramp mode is available for IPG strip
focusing.
Note: For most electrophoresis methods, resistance increases throughout the run. Limiting the
voltage allows for an improving margin of safety over time because the current and power decrease
through the run. Also, the same voltage can be used regardless of the number or the thickness of
gels being electrophoresed.
•Avoid running samples of widely diering salt concentrations or sample buers at the same time or
on the same gel.
Note: Variations in conductivity due to dierences in buer salt concentrations can aect the run
of all the samples run at the same time.
•Properly prepare the samples.
2
PowerEase™Touch HV Power Supply User Guide 9

Install the power supply
1. Check the label located on the bottom of the power supply to ensure that the unit is compatible
with locally provided voltage.
2. Place the power supply on a level laboratory bench and position it such that the On/O switch and
the AC inlet at the rear of the power supply are easily accessible. Keep the area around the power
supply clear to ensure proper ventilation of the unit.
3. Ensure the On/O switch located at the rear of the power supply is in the O position.
4. Attach the power cord to the AC inlet of the power supply.
5. Plug the power supply into an appropriate electrical outlet (use properly grounded AC outlets and
power cords).
6. Connect the leads from the electrophoresis unit to the power supply by inserting the positive lead
(+) into the red output jack and the negative lead (–) into the black output jack.
7. Turn the AC power switch at the back of the power supply On.
IMPORTANT! If restarting the power supply after a previous use, allow at least 10 seconds to
elapse before switching the power supply back on to allow for electrical discharge.
HV adapters
The PowerEase™Touch HV Adapters enable connection of specific electrophoresis equipment to the
PowerEase™Touch HV Power Supply.
Note: When using the adapters, attach to the electrophoresis equipment electrical leads before
inserting the adapters into the power supply. Remove the adapters with the electrical leads when the
run is completed. DO NOT leave the adapters in the power supply when not in use.
Figure4PowerEase™Touch HV Adapters.
Chapter2Methods
Install the power supply
2
10 PowerEase™Touch HV Power Supply User Guide