KERN OZL 441 User manual

OZL-44-BA-e-1510
KERN & Sohn GmbH
Ziegelei1
D-72336 Balingen
E-mail: [e-mail]
Tel: +49-[0]7433- 9933-0
Fax: +49-[0]7433-9933-149
Internet: www.kern-sohn.com
User instructions
Stereo zoom microscope
KERN OZL-44
OZL 441, OZL 445
Version 1.0
01/2015

OZL-44-BA-e-1510 2
GB
KERN OZL-44
Version 1.0 01/2015
User instructions
Stereo zoom microscope
Table of contents
1Before use.......................................................................................3
1.1 General notes.......................................................................................................................3
1.2 Notes on the electrical system ............................................................................................3
1.3 Storage .................................................................................................................................4
1.4 Maintenance and cleaning...................................................................................................5
2Nomenclature .................................................................................6
3Basic data .......................................................................................7
4Assembly ........................................................................................8
5Operation and functionality ........................................................10
5.1 Getting started....................................................................................................................10
5.2 Adjusting the interpupillary distance................................................................................10
5.3 Adjusting the magnification...............................................................................................10
5.4 Dioptre adjustment and focussing ....................................................................................11
5.5 Adjusting the stand............................................................................................................12
5.6 Using eye cups / High Eye Point eyepieces......................................................................13
5.7 Lighting control..................................................................................................................14
5.8 Using external illumination units.......................................................................................14
5.9 Fitting and adjusting a camera..........................................................................................15
5.10 Changing the bulb..............................................................................................................16
6Optical data...................................................................................17
7Features ........................................................................................18
8Trouble shooting..........................................................................19
9Service...........................................................................................20
10 Disposal ........................................................................................20
11 Further information......................................................................20

3 OZL-44-BA-e-1510
1 Before use
1.1 General notes
You must open the packaging carefully, to make sure that none of the accessories in
the packaging fall on the floor and get broken.
In general, microscopes should always be handled carefully because they are
sensitive precision instruments. When using or transporting the microscope it is
particularly important to avoid abrupt movements, as this may damage the optical
components.
You should also avoid getting dirt or finger prints on the lens surface, because in
most cases this will reduce image clarity.
To maintain the performance of the microscope, it must never be disassembled. So
components such as lenses and other optical elements should be left as they were
before use. Also the electrical parts in the base of the device must not be tampered
with, as in this area there is an additional risk of triggering an electric shock.
1.2 Notes on the electrical system
Before connecting to a mains power supply, you must make sure that you are using
the correct input voltage. The information to select the correct power supply is
located on the device, on the rear of the stand base. You must comply with this
information. If you do not comply with these specifications, then fires or other
damage to the device could occur.
The lighting unit must also be switched off before the mains cable is connected. In
this way you will avoid triggering an electric shock.
If you are using an extension cable, then the mains cable you use must be earthed.
When carrying out any procedures whereby you come into contact with the electrical
system of the device, such as, for example, changing the bulb or fuse, only carry out
these procedures when the power is disconnected.

OZL-44-BA-e-1510 4
1.3 Storage
You should ensure that the device is not exposed to direct sunlight, temperatures
which are too high or too low, vibrations, dust or a high level of humidity.
The ideal temperature range is between 0 and 40°C and a relative humidity of 85%
should not be exceeded.
The device should always be located on a rigid, smooth, horizontal surface.
For devices with pillar stands, the microscope holder must not be rotated back too
far. If you do this, there is a risk that the microscope could tip over.
When the microscope is not being used, you should fit the objective cap and cover
the microscope with the enclosed dust protective cover.
If the eyepieces are being stored separately, the protective caps must be fitted to the
tube connectors. In most cases, if dust and dirt gets inside the optical unit of a
microscope this can cause irreversible errors or damage.
The best way to store accessories which consist of optical elements, such as, for
example, eyepieces and objectives, is in a dry box with desiccant.

5 OZL-44-BA-e-1510
1.4 Maintenance and cleaning
In any event, the device must be kept clean and dusted regularly.
If any moisture should be occur, before you wipe down the device you must ensure
that the mains power is switched off.
When glass components become dirty, the best way to clean them is to wipe them
gently with a lint-free cloth.
To wipe oil stains or finger prints off the lens surface, moisten the lint free cloth with a
mixture of ether and alcohol (70 / 30 ratio) and use this to clean the lens.
You must be careful when handling ether and alcohol, as these are highly flammable
substances. You must therefore keep it away from naked flames and electrical
devices which can be switched on and off, and only use it in well-ventilated rooms.
However organic solutions of this type should not be used to clean other components
of the device. This could lead to damage to the paint finish. To do this, it is sufficient
to use a neutral cleaning product.
You could also use the following cleaning products to clean the optical components:
Special cleaner for optical lenses
Special optical cleaning cloths
Bellows
Brush
When handled correctly and checked regularly, the microscope should give many
years of efficient service.
Should repairs still be necessary, please contact your KERN dealer or our Technical
Department.

OZL-44-BA-e-1510 6
2 Nomenclature

7 OZL-44-BA-e-1510
3 Basic data
OZL 441 / OZL 445
Optical system
Greenough
Dimmable lighting
Yes
Magnification ratio
4,8:1
Tube
angled at 45°
Interpupillary distance
55 –75 mm
Dioptre adjustment
On both sides
Gross weight
5,5 kg
Net weight
4 kg
OZL 441
Magnification ratio
4:1
Packing dimensions WxDxH
370x300x475 mm
Product dimensions WxDxH
330x235x380 mm
OZL 445
Magnification ratio
4,8:1
Packing dimensions WxDxH
345x325x475 mm
Product dimensions WxDxH
300x235x360 mm
Standard configuration
Model
KERN
Tube
Eyepiece
Field of view
mm
Objective
Zoom
Stand
Illumination
OZL 441
Trinocular
WF 10xØ 22 mm
Ø 23 –5,5
1x–4x
Pillar
1W LED (reflected light)
0.35W LED (transmitted light)
OZL 445
Binocular
HSWF 10xØ 21.5 mm
Ø 28 –6
0.75x –3.6x
Pillar
1W LED (reflected light)
0.35W LED (transmitted light)

OZL-44-BA-e-1510 8
4 Assembly
The first step is to position the microscope stand on a firm, level surface.
The holder is already on pillar of the stand, but you must always check whether it is
fixed securely and that it is in the correct position (preferably centrally and facing to
the front).
Please see section 5.5 for more details on adjusting the stand.
The next step is to place the microscope head on the holder, by passing the
objective through the holder ring until the rest of the head is above the ring.
You must use the small silver adjusting screw on the front of the holder ring to fix the
head in place.
The alignment of the microscope head is left to the user to do and can be adjusted to
suit the individual application situations.
To make it easier to operate the focus wheels or lighting control for example, we
recommend that you position the head with the tube connectors centrally to the front.
Ideally, the holder and head are then parallel on the central axis of the stand base
(see figure on page 9).
Both eyepieces are already fitted in the tube. You just need to remove the
protective film which covers them.
When doing this, please be particularly careful that you do not touch the optical
lenses with your fingers and that no dust enters the apertures.
In general you should avoid using two eyepieces with different magnifications.
When changing the eyepieces, you must also loosen the small silver screw under
the eyepiece on the tube connectors and once the new eyepiece is fitted, then it must
be re-tightened.
With regard to the use of transmitted light illumination you must make sure that
the frosted glass stand insert is fitted in the centre of the stand base, so that the
transmitted light can be used correctly.
It is always best to fix the different stand inserts using the adjusting screw on the
front side of the stand base.
Additional optional attachments:
The eye cups supplied with the microscopes can be fitted to the eyepieces.
(see section 5.6)
You can fit a C-mount adapter to the appropriate connection point on the top
of the microscope head for trinocular devices (OZM 543, OZM 544). This
enables you to fit and use digital cameras (see section 5.9).

9 OZL-44-BA-e-1510
Assembled stereo zoom microscope

OZL-44-BA-e-1510 10
5 Operation and functionality
5.1 Getting started
After assembly, if the microscope is ready for use, then you must first establish a
power connection using the cable which is connected to the device.
Please see section 5.7 for more details on adjusting the lighting.
If the device has a camera connection (OZL 441), then for binocular use, you must
let the trinocular toggle rod on the left side of the microscope head stay slid-in.
For trinocular use (camera connection) see section 5.9.
Do not forget to remove the cap from the bottom of the objective, so that you will then
be able to see a reflection of the object being observed in the eyepiece.
All important functions which relate to the use of the devices in this document are
described in the following sections.
5.2 Adjusting the interpupillary distance
Different users have different interpupillary distances. So each time a different person
uses the microscope, the gap between the two eyepieces must be re-adjusted.
While you are looking through the eyepieces, use one hand to hold the righthand or
lefthand prism housing firmly.
By rotating outwards or inwards, you can either increase or reduce the interpupillary
distance.
As soon as the lefthand and righthand visual fields exactly overlap each other, this is
the correct interpupillary distance.
5.3 Adjusting the magnification
As the KERN OZL-44 series are stereo zoom microscopes, then you adjust the
magnification using the two zoom adjustment wheels on the lefthand and righthand
side of the microscope head.
Chapter 6 “Optical data” gives information on the possible overall magnification which
the microscope can produce. It will also include the optional use of different
eyepieces and auxiliary objectives.

11 OZL-44-BA-e-1510
5.4 Dioptre adjustment and focussing
A special feature of stereo microscopes is that they are fitted with an optical unit
which has a relatively high depth of field. In order to be able get the most benefit from
this feature, each user must synchronise the focussing mechanisms for themselves.
The steps to do this are described in the following section.
1. Place the object to be observed on the working surface under the objetive.
2. Put both dioptre adjustment rings into the starting position of 0.
3. Use the zoom control dials to set the smallest possible magnification.
4. Look through the right eyepiece with the right eye and bring the object into focus
by using the focus control dials.
5. Now set the largest possible zoom factor.
6. Once again, still only looking through the right eyepiece, bring the object into
focus
7. Then set the smallest possible zoom factor again.
8. If the object then does not appear to be in focus, adjust the focus on the dioptre
adjustment ring of the right eyepiece.
9. In order to get the highest level of accuracy when adjusting the focus, you should
repeat steps 5-8.
10.Afterwards set back to the smallest possible zoom factor.
11.Then look through the left eyepiece with the left eye and use the lefthand dioptre
adjustment ring to also adjust the optimum focus of the object.
12.In this way, the object being observed will be in focus at any zoom setting.

OZL-44-BA-e-1510 12
5.5 Adjusting the stand
Torque of the focus wheels
You can adjust the torque of the focus wheels by turning the ring which is fitted along
the axis of the lefthand focus wheel.
To do this you need a special wrench which is included with delivery. The ring has
holes which the wrench can hook into, so that you can then turn it in the desired
direction.
Depending on the direction of the turn, the torque will be increased or decreased.
On one hand, this function can help to make it easier to adjust the focus and on the
other hand it can prevent the microscope head from slipping down unintentionally. In
this way you can avoid possible damage which could occur if the objective lens and
the object being observed should collide.
Height adjustment
Fixing the microscope holder
With a stereo microscope on a pillar stand, the microscope head can be height
adjusted using the focus wheels and in addition, the microscope holder can be fixed
at any point on the pillar, depending on the application requirements.
To primary way to fix the position is to use an adjusting screw (1) on the holder.
There is a metal ring which also ensures sufficient grip and which is fixed with a
second screw (2) under the holder on the pillar. This metal ring proves to be
particularly useful when the screw (1) on the holder is loosened, to swing the
microscope head to the side.

13 OZL-44-BA-e-1510
5.6 Using eye cups / High Eye Point eyepieces
The eye cups supplied with the microscope can basically be used at all times, as
they screen out intrusive light, which is reflected from light sources from the
environment onto the eyepiece, and the result is better image quality.
But primarily, if eyepieces with a high eye point (particularly suitable for those who
wear glasses) are used, then it may also be useful for users who don’t wear glasses,
to fit the eye cups to the eyepieces.
These special eyepieces are also called High Eye Point eyepieces. They can be
identified by the glasses symbol on the side. They are also marked in the item
description by an additional “H” (example: HSWF 10x Ø 23 mm).
When fitting the eye cups, make sure that the dioptre setting is not moved. We would
therefore advise that you hold the dioptre adjustment ring on an eyepiece with one
hand while you fit the eye cup with the other.
Before using the microscope, users who wear glasses must remove the eye cups,
which you may find on High Eye Point eyepieces.
As the eye cups are made of rubber, you must be aware that when you are using
them, they can become slightly dirty through grease residues. In order to maintain
hygiene, we would therefore recommend that you clean the eye cups regularly (e.g.
with a damp cloth).
Eye cups
High Eye Point eyepiece
(identified by the glasses symbol)

OZL-44-BA-e-1510 14
5.7 Lighting control
The microscopes in the OZL-44 series have an adjustable reflected and
transmitted light illumination.
The power supply is guaranteed by connecting the mains cable and pressing the
main switch on the right, rear corner.
The control wheel on the righthand side surface of the stand base is responsible
both for on-/off-switching and the adjustment of the light intensity of the
transmitted illumination.
The same functions belong to the control wheel on the lefthand side surface of
the stand base according to the reflected illumination.
5.8 Using external illumination units
If, when using a microscope in its standard version, the lighting is not suitable for the
application, then it often makes sense to fit an external lighting unit to overcome this
problem.
The lighting units which are suitable for devices of the OZL-44 series, are goose neck
lighting units (see figure). These are available as LED as well as halogen versions
and also have an on/off switch or different controller.
Typical goose neck lighting unit
Using goose neck lighting units
Depending on the requirements, a goose neck lighting unit is placed next to, in front
of or behind the microscope. For halogen lighting units, the light source is in the
housing of the unit and is emitted through one or several optical fibre cables. For
LED units, on the other hand, it us usually at the end of the cable.
These cables are flexible and therefore offer a large number of positioning options,
so that the object being observed is perfectly illuminated.

15 OZL-44-BA-e-1510
5.9 Fitting and adjusting a camera
(OZL 441)
You can connect special microscope cameras to trinocular devices in the OZL-44
series, so that you can digitally record images or sequences of objects being
observed.
The connection for this is on the top side of the microscope head.
When the trinocular toggle rod is pulled out, the beam path, which normally emits
on the righthand eyepiece, is diverted in the tube, so that it can be used for the
camera adapter connection.
This means that when the device is used in trinocular mode, you will see one image
in the left eyepiece and one on the monitor of the microscope camera.
This means that the 3D effect is lost.
To fit a microscope camera properly, you must use an adapter with a C-mount
thread.
There is already an adapter fixed tot he camerad adapter connection
(white varnishing), which is not suitable for C-Mount cameras. You firstly
have to remove this adapter in order to attache an appropriate one.
In total there are three focusable adapters to choose from (see figure below). The
difference between these adapters is that they have different integrated magnification
(0.3x, 0.5x, 1.0x).
The camera and adapter are then united using the C-mount thread.
C-mount adapter
The image which is shown on the camera connected to the device can often have a
different level of focus compared with the image on the eyepiece. In order to be able
to bring both images into focus, the focus can be adjusted by those adapters
when turning the attached black plastic ring.

OZL-44-BA-e-1510 16
5.10 Changing the bulb
LED
The devices in the OZL-44 range with lighting are all fitted with LED bulbs.
Due to the long service life of an LED lighting system, for these microscopes it will
not be necessary to simply change a bulb.
Problems with the lighting unit would therefore, in most cases, be caused by defects
in the electrical system. If this is the case, then our Technical Service will be able to
help.

17 OZL-44-BA-e-1510
6 Optical data
OZL 441
OZL 445

OZL-44-BA-e-1510 18
7 Features
Model outfit
Kern model
Order
number
OZL 441
OZL 445
Eyepieces
WF 5x / Ø 16,2 mm
○○
○○
OZB-A4101
WF 10x / Ø 22 mm
●●
OZB-A4105
WF 10x / Ø 21,5 mm
●●
OZB-A4106
WF 15x / Ø 15 mm
○○
○○
OZB-A4103
WF 20x / Ø 10 mm
○○
○○
OZB-A4104
Auxiliary
objectives
0,5x
○
○
OZB-A4201
0,75x
○
○
OZB-A4202
1,5x
○
○
OZB-A4204
2,0x
○
○
OZB-A4205
C-Mount
1x
○
OZB-A4809
0,3x
○
OZB-A4810
0,5x
○
OZB-A4811
Stand
Pillar, with LED illumination
(0,35W transmitted + 1W reflected light)
●
●
Stand inlay
Frosted glass / Ø95 mm
●
●
OZB-A4805
black-white / Ø95 mm
●
●
OZB-A4806
External
illumination
For information on external lighting units, please see the Kern Optics main catalogue, from
page 68 and visit our website www.kern-sohn.com
●= Standard-Konfiguration ○= Option

19 OZL-44-BA-e-1510
8 Trouble shooting
Electrical system
Problem
Possible causes
The lighting unit (if fitted) cannot be
switched on
The power cable is either not connected or
not connected correctly
The bulb is not fitted
The bulb has blown
The fuse has blown
The brightness control is set to the lowest
level
The bulb has blown
The wrong bulb has been used
The input voltage was too high
The bulb flickers
The bulb is not correctly fitted
The lamp is worn out
The bulb brightness is not sufficient
The wrong bulb has been used
The input voltage is too low
Optical unit
Problem
Possible causes
You can see two images
The gap between the eyes is not set
correctly
The magnifications of the eyepieces do not
match
There is dirt in the visual field
There is dirt on the object being observed
There is dirt on the eyepiece surface
The image is unclear
There is dirt on the objective surface
The focus wheels are jammed
The torque of the focus wheels is set too
high
The microscope head slips down
while you are viewing the object
The torque of the focus wheels is set too low
Eyes get tired easily
The dioptre adjustment is not correct
The brightness adjustment is not correct
Internationale Temperatur Korrektur Tabelle für °Brix (% Zuckergradient) Das Ergebnis um die folgenden Werte korrigieren (Refraktometer muss korrekt kalibriert sein bei 20°C)
Reading °Brix
0.0 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0
10.0 -0.53 -0.56 -0.59 -0.62 -0.65 -0.67 -0.69 -0.71 -0.72 -0.73 -0.74 -0.75 -0.75 -0.75 -0.75 -0.75 -0.74 -0.73 11.0 -0.49 -0.52 -0.54 -0.57 -0.59 -0.61 -0.63 -0.64 -0.65 -0.66 -0.67 -0.68 -0.68 -0.68 -0.68 -0.67 -0.67 -0.66 12.0 -0.44 -0.47 -0.49 -0.51 -0.53 -0.55 -0.56 -0.57 -0.58 -0.59 -0.60 -0.60 -0.61 -0.61 -0.60 -0.60 -0.60 -0.59 13.0 -0.40 -0.41 -0.43 -0.45 -0.47 -0.48 -0.50 -0.51 -0.52 -0.52 -0.53 -0.53 -0.53 -0.53 -0.53 -0.53 -0.52 -0.52 14.0 -0.34 -0.36 -0.38 -0.39 -0.40 -0.42 -0.43 -0.44 -0.44 -0.45 -0.45 -0.46 -0.46 -0.46 -0.46 -0.45 -0.45 -0.44
15.0 -0.29 -0.31 -0.32 -0.33 -0.34 -0.35 -0.36 -0.37 -0.37 -0.38 -0.38 -0.38 -0.38 -0.38 -0.38 -0.38 -0.37 -0.37 16.0 -0.24 -0.25 -0.26 -0.27 -0.28 -0.28 -0.29 -0.30 -0.30 -0.30 -0.31 -0.31 -0.31 -0.31 -0.31 -0.30 -0.30 -0.30 17.0 -0.18 -0.19 -0.20 -0.20 -0.21 -0.21 -0.22 -0.22 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.22 18.0 -0.12 -0.13 -0.13 -0.14 -0.14 -0.14 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 19.0 -0.06 -0.06 -0.07 -0.07 -0.07 -0.07 -0.07 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.07
20.0 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 21.0 0.06 0.07 0.07 0.07 0.07 0.07 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.07 22.0 0.13 0.14 0.14 0.14 0.15 0.15 0.15 0.15 0.16 0.16 0.16 0.16 0.16 0.16 0.15 0.15 0.15 0.15 23.0 0.20 0.21 0.21 0.22 0.22 0.23 0.23 0.23 0.23 0.24 0.24 0.24 0.24 0.23 0.23 0.23 0.23 0.22 24.0 0.27 0.28 0.29 0.29 0.30 0.30 0.31 0.31 0.31 0.32 0.32 0.32 0.32 0.31 0.31 0.31 0.30 0.30
25.0 0.34 0.35 0.36 0.37 0.38 0.38 0.39 0.39 0.40 0.40 0.40 0.40 0.40 0.39 0.39 0.39 0.38 0.37 26.0 0.42 0.43 0.44 0.45 0.46 0.46 0.47 0.47 0.48 0.48 0.48 0.48 0.48 0.47 0.47 0.46 0.46 0.46 27.0 0.50 0.51 0.52 0.53 0.54 0.55 0.55 0.56 0.56 0.56 0.56 0.56 0.56 0.55 0.55 0.54 0.53 0.52 28.0 0.58 0.59 0.60 0.61 0.62 0.63 0.64 0.64 0.64 0.65 0.65 0.64 0.64 0.64 0.63 0.62 0.61 0.60 29.0 0.66 0.67 0.68 0.69 0.70 0.71 0.72 0.73 0.73 0.73 0.73 0.73 0.72 0.72 0.71 0.70 0.69 0.68
30.0 0.74 0.75 0.77 0.78 0.79 0.80 0.81 0.81 0.81 0.82 0.81 0.81 0.81 0.80 0.79 0.78 0.77 0.75
Temperatur °C