LEITZ LABORLUX S User manual

I 1 Care and Maintenance
Dust pr otection is provided by a fle xib le du st cover which
s hould alwa ys be us ed when the in s trument is not in u se. Th e
stand should be cleaned from tim e to tim e with a linen or lea-
ther c loth; alcohol must nol be used as it altacks th e pai nt , but
petro leum is well suited for clea ning the paint ed surfaces. Pale
spots on th e obj ec t stage c an be rem oved by ru bb ingwith paraf-
fin
oi l or vaseline.
Particular ca re should be taken when undertaking s tudie s
using acids
or other aggre ss ive chemicals. Dire ct contact of
these substances with the stand or optics must be a voided
under all c ircumst ances, and all parts sh ould be carefully
cleaned a ft er u se . The optics must be kept sc rupul ously clean.
Du st ca n be removed from glass s urf aces by mea ns of a dry>
fine-haired brush, blowing gently acros s th e surfa ce whil st
brushing. If the
din is difficult to remove, a cle an cl oth , mois-
tened with distilled water , c an be u sed or , if trus also h as no
eff
ec t, p ur e al co hol may be applied . Particular car e should be
taken when clea ning anti~ref1ection coatings. Th e outer eye-
piece
surfaces an d the front eleme nt s of th e objectives have
coa ting sof approx imat ely the sa me hardn ess asglass and mu st
be correspondingly carefully clea ned .
Objectives s
hould not be screw ed apart during cleaning. If
damage or dirt is noticed ins ide th em, th ey should be re turn ed
to us for repair. Cleaning of the inn er su rf ac es of the eyepiec es
is also advis ed against.
Micros
co pe s b ei ng used in hot and lor humid cJimat es r eq uir e
special
care . It should be ens ur ed that a build-up of fungus
do
es n ot occur, which is managed , in th e first place, by th o-
rough a
nd m et iculous cl ea ning and storag e in a cupboard
whose inside temperature is at least 5 OC above that of the
room.
It mu st al so be provided with ai ring holes, loosely
plugged with co
tton wool or ga uz e as protection against dust. If
this type of st orage is not possible, the microscope mu st be
kept in a clos ed co ntainer with an ade qu ate amount of dr ying
age nt (e.g. silica ge l) . These me asures s hould be ta ken even in
laboratories
with air co nditioning . In warm and dry clima te s)
du st is the greatest ene m y. Th e ins trument should, ther efore,
be cove red with the du st cover immediat e ly after use or clean-
ing and s
tor ed in a c upboard. If a humid period of long er than
one m on th occurs, storage in a warm cu pboar d, as described
above, is d es irable.
Proper handling of the mi croscope will ensur e d ec ades of se r-
vice. If , however, a check over or repair becomes necessary,
please
contact your Leitz agency or our T ec hnic al Service
dir ec t.
Technical Service
Ernst Leitz Wetzl.r GmbH,
postr.ch 2007
D-6330 Wetzl.r ,
West
Germ.ny.
Telex: 483727 eltsc
2

3
Fig. 1
Leitz LABORLUX S with stage no. 11,
condenser no. 56 and binocular tube S.
1 Eyepiece
2 Binocular lube S 45°
3 Analyser slit
4 Objectives
5 Specimen stage no. 11
6 Coaxial drive for moving the specimen
8 Height adjustment
of the co ndenser moun t
9 Condenser
mount with slide changer
7 Screws
to attach object gu id e n o. 12
10 Adjustable height stop of the cond e nse r mount
11 Centring sc rews for condenser mo u nt
12 Condenser no. 56
13 Field di aph r agm
15 Mains switch (concealed)
14 Lamphousing 20

4
2 Assembling the microscope
Mounting the lubes
Press the lever in the direction of the arrow (Fig. 2); the tube
can DOW be in se rt ed into the quick-change mount. O nc e the
tube has been inserted allow the lever to slide back. The tube
can be rotated by3600 and clamped in any position byslightly
pulling the leve
r.
Fi g. 2 Mounting th e tub es
Inserting the eyepieces
The eyepieces are insert ed into the eyepi ece tubes (Fig. 3). For
the LABOR LU X S, Leitz eyepieces are u se d which ar e calcu-
lated for the mechanical tube length of 160 mm. Th es e eye-
pieces are distinguish
ed from those for 17 0 rum tube length by
the additional id ent ification of the fi e ld of view indexafter the
magnification, e.g. JO x /20. If Leitz eyepieces without this iden-
tiflcation oft he field of vi ew index are to be used , a spacer ring
(order no.519653) must be pushed ov er the eyepiece mount on
the side
of the tube.
Fi g. 3 In se rting t he eyepieces

The total magnification of the microscope is given by:
rep rod uc ti on ratio of the ob jec ti ve x eyepiece magni fi ca ti on
(x tube factor).
Example:
25/0
.5 0 objec ti ve
IOx/18 eye pi ece
tube factor Ix
To tal magnification: 25 x 10 x1=250x
Scre wing in the objectives
Screw the o bj ec ti ves into the nosepi ece (Fig. 4) in such a way
thatstepwise magnifica
ti on chan ge is possible (e.g. in the order
4, 10 , 40, etc).
All Leitz microscope obje ct ives from a magnification of 2.5x
and calculated for a tu be length of160 mm ca n be used on the
Leitz L
AB ORLUX S. Please note in th is cootext the field iUu -
mjnation capac it y of the condense r s.
Microscope o bj ec ti ves calculated for 17 0 mm tube length can
be used from 1
6x magnifica ti on.
Fig. 4 Sc r ew ing in th e objectives
I 5

6
Identification markings on the objecti'ves
(Fig. 5)
1 Mechanical tube l engt h
The di stance in mm from the ob je ctive sho ulder to the edge of
the tube .
2 Coverglass
The engraving 0.17 is the thick ne ss of the coverglass.
A
dash (-) instead of a number indicates that the objective can
be us ed for sp ec imens with or without a coverglass.
3
Field Oatness of the objective
EF objectives a
re systems with a more or less flat fi e ld of view
of up to 18 mm intermediate image diameter.
PLAN objectives are systems with a flattened fi eld of vi ew up
to 22.5
mm interm ed iate image di ame ter.
Fig. 5 Objective identification
If there is no indica tion of field fl at tening, the objecti ve is an
achromat with optimum correction, for use with up to 18 field
of vi ew inde x.
4 Reproduction scale in the int er mediate image
Size ratio between the intermediate
im age and the object, e.g.
10 :1
5 Numerical aperture
Physical identification
of the objective's resolving power
6 Immersion medium
Immersion media can be, for example.o
il .water (W) orglycer-
ine (GLYC). Th e objective must always be used with the
engraved immersion medium.
Bef ore the immersion objective is focu se d, a drop of im mer-
sion medium should be applied to the obje ct without air
bub bles.
7
Ph ase cootrast objectives
Objectives with
ph ase ring for phase contrast observation.
The se objectives have green writing (w ith the exception of
achromats with black sleeves). The label for the condenser-
side annular stops necessary for these ob jective s (e.g.
PHACO 3) in a slide or in the turret platc of tbe UKL phase
contrast condenser (e.g. PHACO 3= set turret plate to 3) is also
to be found on th e phase c ontra st obj ec tives.
8 Colo
ured ring indicating the ob jective magnification
9 Immersion objectives

Allaehing Ihe objeel guide
Th e o bj ecl guide no. 12 for left- or right-hand ope ration is
allached to the left
or right of the specimen stage with tw o
screws (6.l).
Inserting the condensers
Rotate the condenser clamp (2) until the dots on the knurled
knob and on th e conde nser mount coincide. Th en lower lhe
condenser USing the knob (7.1) until the condenser (7.6)can be
eas ily pushed into the mount as far as the stop. Now turn th e
co nd en se r cl amp in the direction orthe a rr ow. Th e co ndenser
is now firmly fi xed in the slide chang er. Con de nse rs LK and
UKL are inserted in Ih e s ame way as condenser no. 56 (illus-
Irated, 7.6).
Fig. 6 Attaching th e object guide
1
Fi xing screws for o bj ecl guide no. 12
-
Condenser no. 56
With th e two·lens co nden~e r n O. 56. homogeneo us illumina-
ti on is pns>ible for a ll object fields oro bj cc t;ves from 4x magni-
fication at fi e ld of vi ewindex 20 with o ut additional swinging in
and out of the front lens.
Cond enser no. 56 works with the numerical aperture A 0.90. If
oil cap n
o. 512652 is screwed on , the num ericalaper tu re can be
ra ised to A 1.2 5 when L ei tz i mme rsion oil is applied betw een
th e conden se r and the specimen. To screw th e oil cap on, the
protecti ve ring mu st be sc rewed ofT wi th a knurled ring.
F ig. 7 Inserting the c on denser
1 Height adjustme
nt of the condenser holder
2 Condenser clamp
3 Condenser holder
4 Cen
tr in g screws for c ondenser mount
5 Adjustable height stop for the condenser holder
6 Condenser
no . S6
.,.-
7

8
The aperture diaphragm is adjusted with the lever Th ere is a
scale for reproducible s
etting of the condenser diaphragm.
The slit at the side is intended for the in se rtion of slides with
annular stops for simple darkfi e id or ph ase contr as t illumina-
tion. These
can only be taken o ut of condenser n o. 56 again
after the le ver has been pressed.
Condenser
LK
The LK condenser has a hinged co ndenser top S 1.1. When the
con denser topisswung o ut , th elower part ofthe cond eo ser can
illuminate aU obj ec t fi e ld s of o bj ectives from 2.5x upwards
with field
of view index 20. Wh en using ob je ctives of lOx and
higher the condenser top must be swung in . This is done with
the handle (8.2).
Fig . 7b Cond ens er no. 56
I. Protective ring
2. Locking lever for f> hde with dif Tu sion disc and annul ar stops
3. Slil ror slide with diff usion di sc and annular st op s
4. Lever for adjustment of aperture diaphragm
5. Dovetail guide
With co ndenser top A 0. 90 S 1.1 swung in, the co nden ser LK
works with
the numerical aperture A 0.90. Th e image of the
field diaphragm is produ ce d with the cond enser top S 1.1 in a
gla ss me dium 1.2 mm over th e stage surfac e.
The condenser top of the LKcondenser is interchangeable via
a thr ea d with other conden se r tops with a higher num erical
ap ~ rlur e or tops with a long er working distance. Darkfi eld con-
dens er to ps D 0 .8 0 or D 1.19 can also be screwed on instead of
the S 1.1 standard top. Furth er information on condenser tops
can be found in the table on page 10.
The aperture iris diaphr a gm is adjusted with thc lever (8.3).
Ther e is a sc ale for reproducible se tting of the condenser
diaphragm.
Fig. 8 Co nden s er LK
t A0. 90 S I.l Co nd enser top
2 H<lndl e to swing out th e co nd en se r top
J Lev er for adju stment of t he il perture dia ph rn gm

Condenser UKL
Its performance features in brightfJe ld are the sam e as those of
the condenser no. 56.
The annular s lO PSnecessary forphase contrast ordarklield illu-
mination are situated in a turret plate (9.4) for qu ic k chaoge.
P
os iti on " H" (9.3) of the turret is for brightfle ld (Hellfeld)
investigations. Positions I, 2 and 3 accommodate the annular
SlOPS PHACO 1,2 and 3. These can be aligned to th e phase ring
of the objective in li se by pushing in the two centring screws
(9. 2) . Positi on 4 is occupied by an annular st op for darkf.eJd
wi th o bj ectives from lOx to 40x.
Th e wh ee l (9.5) is for ad justing th e ape rtur e di aph ragm, on ly
necessary for brightfield investigations.
It is fully opened for
phase contrast
or darkfield (PH position).
F ig . 9 UKL conden se r
1Adjustment tel es co pe for ct: ntri ng the a nn ular stop s
2 Centring sc r ews (pr ess io to u se)
3Turret pl ate p os ition indi ca ti on
4 Tu rr et pla te
5 Adjustment wheel wi th sca le fo r ape rt ure diaphragm
Darkfield condensers 00.80 and D!.l9
The co ndenser D 0. 80 is intend ed for darkfleld investigations
with
lOx to 40x objectives. Imag e contr as t is optimised he re as
compar ed w ith th e darkfieJd produced w ith annular stop s.
The conden se r D 1.1 9 has an oil imm ersion cap and is used
toge th erwith oi l immersion object iv es. The darkfield con den-
ser caps are also available without bottom parts and can be
screwed onto the b ottom part of th e LK condenser.
Fig. 10 Darkfield objec tiv e and funnel stop
J Co ndensertop D 0.80 - 0.95 SI.J
2 Oil co ndenser top D 1. 19 - 1.44
3 Lower pari of co nd en se r with dovetail guide
4 Sp ec ial darkfield o bj ec ti ve EF 100 / 1. 25 -0 . 60 Oel wilh built-in i ri s
diaphra
gm
5 Knurled ring for adjustment oflhe iris di aph r ag m
6 Funn
el slop for th e o il immersion ob jective
9

10
Condenser LK
Condenser
Top
Top in/oul
Use
0.90 S 1.1
Out
With objective aperture <0.25
0.90 S 1.1 In
With objective aper ture >0.25
OEL 1.32
In
( Imm ersion o il on fro nt element.)
With OEL 100/1.32 objective
0.70 S4 In
Inter cept distance 4 mm. With spec imen slides of
thickn ess> 1 mm .
0.55 S 15
In
Intercept distance 15 mm . With specimen slides of
thickness> 6 mm.
DO.80-0.95
Conde nser top in light path Darkfield. With objecti ve apertures <0.75.
D 1.19-1.44
Condenser top in light path
(Immersion oi l on front e lement ) Darkfield.
With objective apertures < 1.10.
UKL ul)iversal condenser for phase contrast
Condenser Revolver Objective
Application
top
setting with
engraving
0. 90 S 1.1 H
I
2
3
5
AU objectives
PHACO I
PHAC02
PHAC03
AU objec tiv es
with apertur e
< 0.75
BrighLfield
Pha se contrast
Phase contrast
Phas e contrast
Darkfteld

Attaching the I.mphousing 20
Lamphou sing 20 is plugged into the guide sockets situated at
the back of the stand and secured with the screw (11.1) (you may
find it handy to use a coin). Then connect th e microscope via
the conn e cting soc k. et (11.2) with the mains cable to the mains.
The switch (lU) is For switching th e illumination on and off.
The lamp intensity can be re gulated with th e k.nurled k.nob
(11.4) .
Fig. It Att ac hing iamphollsing 20
1Fixing s
cr ew
2 Connecting sockel ror mains cable
3 On /offswilch
4 Intensity regulation
Changing the lamp
Pr es s the la mp so ck. et (12.1), unlock by turning to the le ft and
take it out of the lamp housing. Remov e the def ect lamp. When
inserting the new la mp , do notremo ve its prot e cti ve wrapping
until the lamp is in po s ition . Return th e lamp socket to the lam-
phollsin g, press in and lock in place by turning to the right
Fi g. 12 Changing th e lamp
I Lamp soc kel
2 Int ensi ty reg ulation
11

Operation of the microscope
F ocu sing the image
P la ce a specimen on the stage or slide it i,nla the mounting
plate
(13.1) if available.
Spe
cimen stage no. II (1.5) can be suppli ed with two clips to
sec
ure the specjmen. Instead of these cl ip s, ob je ct guide n o. 12
can be attached to the left or right of the stage for left-or right-
handed
operation (Fig. 6).
It bas an
adjustment range of76x 26 mm.
P recise movement of the specimen wlth in a r ange of
76x 52111m is possible with the me c ha ni ca l stag e no. 18
(Fig. 13).
The object guide no. 12 and mechanical st age no. 18 have a ve r-
nier scale (1 3.2) for adjustmen t of both th e x and the yax i s.
Fig. 13 Mechanical stage no . 18
1 Specimen mounting plate
2 Vernier scale
t2
This ena bl es ce rtain pa rt s of th e sp ec imen to be se t reproduc-
ibly.
Turn
in a m ed ium-power objective e .g. EI' 10/0. 25 by r otating
the n ose pi ece. Switch on the illumin al ion (11.3 ) and r eg ulate
the
inten sity by turnin g knob (12.2).
Turn the screw (7.5) by approx. 5 ro tations to the left and u se
the height adjust ment (7.1) to mov e the conde nse rto the upper
stop ne ar th e specimen.
With the LK co nden se r, the co n de n se r lOp (8. 1) is sw ung into
lh e illumin ating light path with the handle (8.2 ). [fyou change
to objectiv es of less than lOx magnification afterwa rds , swing
the co n dense r top out of lhe illumin ating li ght path.
Ope n the a perture diaphragm (8.3) and the fi eld diaphr agm
(1.13) .
Adju stment of the binocular tubes
If u si ng the binoc ul ar tubes, adjust the interpup i ll ary distance
until the im ages for b oth eyes compl etely cov er ea ch oth er an d
appe ar as a s in g le circ ul arimage. Then tran sfer the interpupil-
l ar y distanc e (14 .1) to th esea l es (14 .2) of the two eye pi ec e tub es.
How
to compensate for vision defects:
Look throu gh the right-hand ey epiece with yo ur right eye an d
focus the specimen with the
fin e drive. Th en watch the sa me
part of tbe s pecimen with yo ur left eye and ro tate the left-hand
eye piece tube until th e sam e part of th e object is in sha rp focus.
Do not u se the fine drive for this. This setti ng sh ould be
rechecked after centI ation of the condenser.
Wh en us ing the bin oc ular FSA or FSA-R pb oto tub es (F ig. 14)
the tube l eng th is automatically com pensated when the ey e-
p ieces ar e se t at the reqllired interpupillary di stance.

Th e sy mb ols on Lh e rotary kn ob of the bin oc ular photo Lub es
mean:
100 % light to the eyepieces
L 50% light to the eyepieces,
50% to the photo ex it
10 % light to the eyepieces,
90 % La the photo exit
Th e viewing angle of the binoc ul ar SV and FSA-VR tub es can
be adjusted by tilting
Lhe front part of the tube between 0 and
40°, The user can thu s set or alter the vi ewing height to suit
himself.
Fig. 14 Selting the b inon ll ar Lube s
Centring (he condenser and setting the fieJd diaphragm
Focus th e specimen using the coarse and fine drive.
1. Close the fie ld di aphragm. Loosen tbe stop screw and raise
the co nd en se r to it s upperm ost position with the height ad-
justment ( 1. 8).
2. Turn the co ndens er stop screw (1.1 4) to lower the conde nser
until the edges of the field diaphragm appear sharp.
3.
Cen tre the image of the fi eld diaphragm wi th the tw o ce n-
te ring screws.
4. Open the field diaphragm until i(just disappears from the
field
of view.
Fig. 15 Centering the condenser and se ll ing the field cli<lphrtlgm
13

14
Use of the field diaphlllgm
I fop timum contrast is to be attained, it isimportant to i.llumin-
ate only tbe part of the specimen that appears in the image.
Therefore the
fi eld diaphragm is only opened as far as the field
of view. For this reason, a change of magnification always
necessitates matching the field diaphr agm to the ob ject field.
U se of the aperture diapbragm
The more tbe aperture diaphragm is narrowed. the higher the
image
contras t Axial resolving power (image sharpness in the
direction of the optical axis) also in creases. However, exces-
~ive closure of the aperture diaphragm has an adverse effect on
lateral resolving power (selectivity).
The best optical pe rformance is ob tained when the ape rtur es
of th e objec ti ve and the condenser are the sa me.
A visible deterioration of r eso lv in g power is see n wb en tb e
aperture dia pbr agm is closed over '13 of the ap ertur e of the
objec
ti ve. and tbis sbould be avoided as far as possible. To
check this.
an eyep iece must be taken out of the ey epie ce tube ,
th e ape rture diaphragm narrowed until its im age covers 1 13 of
the illuminated rear lens area. Replace the eyepiece.
Ifnecessary, the aperture diaphragm can be na rr owed further
f or objects with weak contrast.
Once determined. the aperture diaphragm s ettin g can be
reproduced with the aid of the scales on the brightfield and
phase contrast condensers.
Note:
Th e aperture diaphragm should not be used to set image inten-
sit
y. This is done with the rotary kn ob (12.2) for regul at ion of
lamp intensity, or for photomi crography, with neutral light-
blocking filters.
Oil immersion objectives
Oil immersioo objectives are labe ll ed with th e additional
engraving "OEL" and a black ring on the lower edge of the
objective mount. The y must always be used with immersion
oil. Before the oil
immersion objective is screwed into the
nosepiece a drop of immersion oil must be applied to thespeci-
men. Check that the imm ersion oil is free from air bubbles.
The immersion oil has almost the same refractive index ne=
1.515 as the coverglass and the front l ens of the microscope
objective. The focal length and the working distance of an
immersion objective are usually very small. For this reason,
working with oil immersi
ons requires great ca re.
For routine work, the ordin ary 0.90 condenseris adequate even
for oil immersions. However, if the full aperture of the immer-
sion objective is to be used, e.g. for very fine structures, the
co nd ense raperture must also be enlarged. For co ndenser no.
56 this can be done by scr ew ing on a 1.2 5 OEL co nden ser cap,
or for the LK condenser, an
APL OE L 1.32 S 1.1 co nden ser top.
In the se cases, immersiono il mustal so be applied between th e
con den ser cap/top an d tbe underne ath of the sp ec imeo slide.
After the investigation, aU surf aces where immersion oil has
been applied must be carefu ll y cl eaned. Use a so ft cloth mois-
tened with alcohol or ben zine.

Transmitted light darklield with the LK condenser
For darkfield investigation s, condenser top D 0.80 - 0.95 IS
used when the objec ti ve has an aperture of less than 0.75 and
condenser top D 1.19 - 1.44 when the objective's aperture is lar-
ger than
0.75 .
For apertures greater than l.ID, insert a funnel stop into the oil
immersion objective or use an objective with buill-in iris
diaphragm. Separate bottom parts are also available for the
darkfield con denser tops, which are pushed into the condenser
mount instead of the brigblfield or phase contrastcondensers.
Focusing tbe darklield image (D
1.19 -1.44 and D 0.80 -0.95)
Put a s pecim en on the stage. Turn the condenser stop screw to
the right as far as the stop. Inse rt th e condenser and raise to the
condenser stop.
If using the D 1.19 condenser top, first app ly a drop of imm e r-
sion oil to thesurface of the condenser (taking care to avoid air
bubbles) and then raise until the drop of oil touc he s the under-
neath
of the specimen slid e. Thiscan be seen by a brief lighting
up
of the slide.
Bring the specimen inlo focus. (Use Ihe
10 /0. 25 or 16/0.40
objective). Close the field diaphragm. Raise
or lower the con-
denser
by adjusting the condenser slop screw ( 1.10) to the left
and using the condenser drive
(1.8) until theedges of the diaph-
ragm are as s harp as possible when the specimen is obs erve d.
Move the image of the di a phr agm into the centre of the field of
view with the tw o centring screws (1.11).
Open Ihe field diaphragm until it just disappears from thefield
of view.
Phase contrast or darkfield illumination with condenser 00. 56
Condenser no. 56 can be fi tted wi th annular stop slides DF I
PHACO for
ph ase contrasl or darkfield ill umination:
-
Centre the condenser with a brightfie ld spe cimen
-Screw in phase contrast objectives
-Open the aperture diaphragm
-Push the slide with the appropriate annular stop (chart on
page
16) into the condenser (Fig. 16).
The DF 155-56 annular st op slide is also av ai lable for conden-
ser no. 56, with which simple darkfield i
ll umination can be
obtained using Objectives from lOx to 40x.
Fig . 16 In sertion of th e sli de with annular stop in to condenser no. 56
15

16
Annular
stop EF objectives PLAN objectives
I 10/0.25 PHACO I PLAN 10/0.25 PHACO I
2 25/0.40 PHACO 2 PLAN 20/0.40 PHACO I
40/0.65 PHACO 2 PLAN 40/0.65
PHAC02
3
10011.25 PHACO 3 PLAN
10011.25 OEL PHACO 3
Phase conlr.lsl objectives Phase contrast with the UKL universal condenser
[nsert the UKL condenser into the condenser mount (1.9) and
bring to its highest position with the vertical adjustm en t (1.8).
Set the
aperture diaphragm (9.5) to "10" (green). Put a speci-
men on the stage. Turn in the 10/0.25 PH ACO I ob j ec tive.
Set the annu lar stop turret at position Iand focus (he spec imen
with the coa rse and fine drive. Cl ose th e field diaphragm. Set
Ihe condenser with the vertical adjustment (1.8) so that the
ed ges of th e clo sed fi e ld diaphragm are in sharp foclis. Centre
the image
of the field diaphragm with th e two centdng screws.
Open the tield diaphragm until it ju st dis appe ars from the field
of view. Remove an eyepiece from the e yepiec e tube and insert
the focus
in g telescope. Loo sen t he clamp ring on th e focusing
telescope and adjust the eyel ens until li ght and pha se rings are
in sharp focus. Using the cenlring screws (press in a nd turn)
adjust the lig ht ring so that it is exac tly covered by the phase
ring of the objective. Ce nlralion must be c<:Irried out on ce for
all
objective/annul ar stop combina tions <:Ind is th en retained
for all further settings.
Fig. 17 Phase ring and annular stop as s een through the fo cusing
telescope.
(]

Polarisation
Remove the protection piece fr om the analyser slit (18.1) and
push
the slide with the analyser (18.2) into the slit (18.1).
In sert the filter holder into the field diaphragm mount so that
the open part faces the user. It should click into place.
Push
the polariser (1) into the low er slit of the filter holderand
turn until the field of view, observed without a spec im en in
place, appears at its darkes t.
If desired, a compensator ( ,\ or Al4 plate) (19. 2) can be used in
th e sec on d filter slit above th e polarise r.
Fig. 18 Inserting the analyser
Fig.
19 A rrangeme nt or the poiariser
I Polariser
2 Compensator
3 Holder
17

18
Incident light fluorescence
Assembly of Ih e equipmenl
Attach
Ih e lamp hold er ( 21.10 ) to Ih e upper pa rt of the st an d
with 2 Allen screws.
Unlock the obselVa
ti on tub e ( 21.13 ) and remove from the
stand.
Pl ace the PL O EMOP AK (2 1.5 ), with light shield
attach ed, on the st an d. Push the li ght shield (2 1.9 ) into the lamp
ho
ld er.
Insert th e observation tube into the tub e mount of the PLO E-
MOP A K. Th is is do ne by pressing the lever (2 L1 3) to the back
a nd releas in gon ce th e lubusis in p osi ti on.Att ac h th e la mph ou -
sing ( 21.11 ) to the lamp holder, making sure that the clamping
leveris in a ve rt ica l posi ti on. Cl a mp by tu rnin g th e leverto th e
lel 1. Remove the condenser and put th e li ght trap in its place
(F ig. 20 ).
Fig. 20 ln se rLin g th e light tr ap
F ig . 21 Leitz L AB ORLUX S with I-Lambda PLOEMOPAK incide nt
li ght fluor esc ence illuminator
I G
ro u nd gl ass screen fo rchecki ng la mp cenl ra ti an
2
Or ientation m ar ks f or co rrect inse rt io n of th e fi lt erchangi ngdevice
3 Loc
kin g mecha ni sm of the fillerc han gi ng device
4 F
il ter chan gi ng device
5 1- ,\ PLOEMOPAK
6 Fi eld diap hr ag m
7 Excita ti on li g ht barrier
8 SO38 red a bs o rpti on filt er
9 Li ght s hi e ld
10 La mp holderf or lamphollsing 10 2Z
II Lamphollsing 10 3 Z with 100 W mercury maxi mum-pr ess ur e lamp
12 Light trap
13 Lever for opening th e tube mount

Q)~
. ~®
®
19

20
Fig. 23
Fig. 22
Remov ed filler changing dev ice with key for loosenin g/ ti ghteni ng the
Removing t he -filler chang in gd ev ice to ch a nge the filter syste-m filler block
,