Levenhuk MED 12M Microscope DigitalCamera User manual

Levenhuk MED 12M Microscope Digital
Camera with 9.7" LCD Screen
User manual
EN

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Never, under any circumstances, look directly at the Sun, another bright source of light or at a laser through this
device, as this may cause PERMANENT RETINAL DAMAGE and may lead to BLINDNESS. Children should only use the
device under an adult’s supervision.
General information
Levenhuk MED Digital Camera is a professional accessory for trinocular microscopes. Its 9.7" color LCD touch screen
and 12Mpx digital camera allow you to conduct high-precision research in real time, and save images and video for
further use.
The kit includes
Digital camera with an LCD display, C-mount, AC adapter, USB cable, user manual, and warranty slip.
Parts of digital camera
1. Microphone
2. USB port
3. USB mouse port
4. Headphone jack
5. AC adapter socket
6. MicroSD (TF card) slot
7. HDMI out
8. Power on/off
Caution! Please remember that mains voltage in most European countries is 220—240V. If you want
to use your device in a country with a dierent mains voltage standard, remember that use of a
converter is absolutely necessary.
Installing a digital camera
LCD screen digital camera is installed on the third vertical tube of trinocular microscopes. Assemble and set up your
microscope. Insert the digital camera into the C-mount, then insert the C-mount into the trinocular tube and tighten the
locking screw. Connect the supplied AC adapter to the camera and to the AC network. Press the power button and the
camera will be ready to work. Use the supplied USB cable to connect the camera to your PC.
Embedded software
The embedded application is designed for using Android digital cameras with microscopes. With this application, you
can change image parameters, measure objects and save images for further use. In addition, you can perform particle
analysis with its image processing feature. Like all Android applications, this application features a user-friendly
multilanguage interface.
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Capturing and playback tools
Press the Snap button to take a photo. Select the image size in the Settings section.
Press the Record button to start video recording. To stop recording, press the same button
once again.
If the original size of the video is smaller than 1080p, then the size of the recorded video will
be the same as the original. If the original size is bigger than 1080p, the recorded video size
will be reduced to a size smaller than 1080p.
Press the Playback button to open the last image captured or to play the last video recorded.
The image will be opened in the Image Analysis window.
Camera display interface
The screen is divided into two parts:
1. The left part is the Preview Image. It displays the current image provided by the camera.
2. The right part is the Tool Panel. It contains a number of tools for image capturing, changing parameters,
measuring, etc. Use the right side of the screen to perform the following actions:
• Effect: Settings of the camera parameters
• Measure: Measuring tool
• Playback: Images and video playback
• Snap: Image capturing
• Record: Video recording
• Setting: Setting and changing parameters

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Capturing and playback tools
Measurements
The Effect panel allows you to edit the captured image, if
necessary.
Exposure
Auto Exposure: The brightness of the image will be automatically
adjusted.
Manual mode: You can set the exposure time and gain.
Color temperature (White balance):
Press this icon to set the white balance automatically. For
manual settings, use the slider tool on the right.
Contrast: Use the slider tool to adjust the contrast.
Saturation: Use the slider tool to adjust the saturation.
Sharpness: Use the slider tool to adjust the sharpness.
Gamma: Use the slider tool for the gamma correction.
De-noise: Use the slider tool to adjust the noise level.
Press this icon to reset all the settings to the default
parameters.
On the Measure panel, you can select a tool to conduct a
measurement.
Before you start, you need to calibrate the ruler. You also need to
calibrate it after changing the microscope objective and/or camera
resolution.
1. Calibrations
Go to the Measure panel, and press Add button to start a new
calibration process. Press Edit button to make changes in
precious calibrations.

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Open calibration menu:
Follow the instructions:
Adjust the yellow ruler to where its ends are on the physical ruler’s scales. The ruler on the screenshot has 0.01mm
scales, and each big grid equals 10µm, so two big grids equal 20µm.
Enter the name for the calibration and actual length of the ruler. In the example, the name of the calibration is
“40x”, which corresponds to the magnication value of the chosen eyepiece, and “20µm” is the actual length of
the chosen ruler.
Press Calculate to get the calibration value for the current eyepiece and image size. Press Add to save the
calibration.

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2. Measuring tools
You can use a wide range of measure tools (rulers).
3. Use of the measuring ruler
Choose a calibration (the chosen option will be displayed as a black print on the white background).
Icon Measure tool Action
Point counting Set the starting point of measuring on the photo
Line Measure the distance between two points
Rectangle Measure the width, length, and area of the rectangle
Circle Measure the area of a circle
Cros Measure the cross hair
Angle Measure the angle
Two Circles Measure the distance between the centers of two circles
Perpendicular Measure the length of a perpendicular line
Concentric Measure the radius of two concentric circles
Text Annotation Add a comment to an image
Option Change the width or the color of the ruler’s scale, and the size or the
color of the text
Export Export the image with measure marking
Delete Delete the chosen ruler
Delete Delete all

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Choose a ruler from the side menu, and it will appear in the image.
Use the measuring ruler to measure the physical ruler.
In this example, 1.5 large grids were measured: the measuring ruler shows 14.910µm, which conrms the correctness
of the measurements.

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Image analysis
The images are analyzed with the Android application YWImage, which uses OpenCV (Open Source Computer
Vision Library). The application version 1.0 supports such functions as gray scale, contrast, binarization, and particle
analysis.
Let us consider an example of particle analysis.
Image uploading. Click the camera icon in the upper right corner of the screen. You can select an existing image or
take a picture with the camera.
Convert a color photo to Grayscale mode. Choose Grayscale from the left menu and press Apply to conrm
converting.

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Contrast and brightness adjustment. Adjust the contrast and brightness of the black and white image, increasing the
difference between objects and the background.
Binarization. Using the binarization function, you can convert a color image or an image in grayscale to two-color
(black and white) by setting the minimum and maximum thresholds with which the brightness of each pixel will be
compared.
All pixels within the threshold range will be highlighted in red. After executing the binarization, the RGB-value of the
red areas will be set to (0, 0, 0), the RGB-value of the other sections will be (255, 255, 255).
Note: Red areas should not overlap.

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Particle analysis. After executing the binarization, open the particle analysis tool.
Press the button to set the maximum value to innity. Select the Show Outlines check box to show the
boundaries and number of particles after the analysis. Click Analyze Particles to begin the analysis. The result will be
shown below.
Click the CSV button to export the data to the report le, which will be saved here by default: /mnt/sdcard/Image/
Reports.
First of all, if some noises were not deleted during binarization, you need to specify a particle size range. The
designation of the particle size range will make the result more accurate.