Major Science SmartView Pro 2300 User manual

SmartView Pro 2300/2400
Imager System
Instruction Manual
Catalogue No. UVCI-2300
UVCI-2400
www.majorsci.com
[e-mail]
Version 07A
Revised on : 2018/11/07

1
Packing List
Signed by:Date:
Major Science is liable for all missing or damaged parts / accessories
within 7 days after customer received this instrument package. Please
contact Major Science immediately regarding this issue. If no response
within such time period from consignee party, that will be consignee
party’s whole responsibility.
Standard Package
UVCI-2300
UVCI-2400
SmartView Pro 2300 Imager system
-1 x 2.0MP CCD camera and lens
-1 x 312(302)nm UV transilluminator
-1 x Epi White Light Module
-1 x UV viewing window
SmartView Pro 2400 Imager system
-1 x 2.0MP CCD camera and lens
-1 x 470nm blue transilluminator
-1 x Epi White Light Module
-1 x Blue viewing window
-1 x Power Cord
-1 x Instruction Manual
-1 x SmartView 1D Analyze Software CD
-1 x Stylus Pen
-1 x Close up lens
Optional Package
UVCI-2300
UVCI-2400
White Light Plate
- 1 x White Light Plate
- 4 x M3 screws
White Light Plate
- 1 x White Light Plate
- 4 x M3 screws
Blue Light Plate
- 1 x Blue Light Plate
- 4 x M3 screws

2
Table of Contents
Packing list.....................................................................................................1
Warning...........................................................................................................6
1Introduction....................................................................................12
1.1 Overview ..............................................................................................12
1.2 Feature.................................................................................................12
1.3 Components Guide ..............................................................................13
2Technical Specification.................................................................14
3Installation Instructions................................................................16
3.1 Installing Blue Light Plate (Optional) ....................................................16
3.2 Installing White Light Plate (Optional)...................................................19
3.3 Installing SmartView 1D Analysis Software..........................................22
3.3.1 IPAddress Setup...............................................................28
3.3.2 IPAddress Confirm ...........................................................34
4Operation Instructions..................................................................38
HMI Interface (UVCI-2300)........................................................................38
4.1 Start the System...................................................................................38
4.2 Select the UV Intensity.........................................................................40
4.3 Add Info................................................................................................40
4.4 Title.......................................................................................................41
4.5 Adjust the Volume of Exposure.............................................................41
4.6 Capture.................................................................................................43
4.6.1 Save Pictures To Remote Computer ......................................44
4.7 Default Setting......................................................................................46
4.8 System Setup.......................................................................................48
4.8.1 Touch Screen Calibration........................................................49
4.8.2 System Information.................................................................49
4.8.3 System Time...........................................................................50
4.8.4 System Upgrade.....................................................................50
4.8.5 Optional Devices.....................................................................51
4.8.6 Copy to USB...........................................................................51

3
4.8.7 CopyAll to USB......................................................................51
4.8.8 Delete Selected......................................................................52
4.8.9 Delete All ................................................................................52
4.8.10 Screen Saver........................................................................52
4.8.11 Create Folder........................................................................52
4.8.12 Remove Folder.....................................................................53
4.8.13 Network Settings...................................................................54
4.9 Reverse out..........................................................................................54
4.10 Freeze................................................................................................55
4.11 Turn ON/OFF the Lighting Modules....................................................56
4.12 Print....................................................................................................57
4.13 Load and Edit the Picture...................................................................57
4.13.1 Anti-white..............................................................................59
4.13.2 Flip upside down...................................................................59
4.13.3 Flip horizontally.....................................................................59
4.13.4 Turn counterclockwise..........................................................60
4.13.5 Turn clockwise......................................................................60
4.13.6 Brightness adjust..................................................................60
4.13.7 Crop......................................................................................61
4.13.8 Text.......................................................................................61
4.13.9 Undo and redo......................................................................63
4.14 Signals Indication...............................................................................63
4.13 WARNINGS:.......................................................................................63
HMI Interface (UVCI-2400)........................................................................64
4.1 Start the System...................................................................................64
4.3 Add Info................................................................................................66
4.4 Title.......................................................................................................66
4.5 Adjust the Volume of Exposure.............................................................67
4.6 Capture.................................................................................................68
4.6.1 Save Pictures To Remote Computer ......................................69
4.7 Default Setting......................................................................................71
4.8 System Setup.......................................................................................73
4.8.1 Touch Screen Calibration........................................................74
4.8.2 System Information.................................................................74
4.8.3 System Time...........................................................................75
4.8.4 System Upgrade.....................................................................75

4
4.8.5 Optional Devices.....................................................................76
4.8.6 Copy to USB...........................................................................76
4.8.7 CopyAll to USB......................................................................76
4.8.8 Delete Selected......................................................................77
4.8.9 Delete All ................................................................................77
4.8.10 Screen Saver........................................................................77
4.8.11 Create Folder........................................................................77
4.8.12 Remove Folder.....................................................................78
4.8.13 Network Settings...................................................................79
4.9 Reverse out..........................................................................................80
4.10 Freeze................................................................................................80
4.11 Turn ON/OFF the Lighting Modules....................................................81
4.12 Print....................................................................................................82
4.13 Load and Edit the Picture...................................................................82
4.13.1 Anti-white..............................................................................84
4.13.2 Flip upside down...................................................................84
4.13.3 Flip horizontally.....................................................................84
4.13.4 Turn counterclockwise..........................................................85
4.13.5 Turn clockwise......................................................................85
4.13.6 Brightness adjust..................................................................85
4.13.7 Crop......................................................................................86
4.13.8 Text.......................................................................................86
4.13.9 Undo and redo......................................................................88
4.14 Signals Indication...............................................................................88
5SmartView 1D Analysis Software.................................................89
5.1 Process ................................................................................................89
5.2 Editor..................................................................................................101
5.3 Selecting the Image Lane...................................................................102
5.4 Analyze...............................................................................................104
5.5 Summary............................................................................................113
Dot Blot Analysis Sample .........................................................................120
6Troubleshooting Guide ...............................................................135
7Cleaning & Maintenance.............................................................137
7.1 Replacing the Fuse ............................................................................137
7.2 ReplacingAmber Filter on Viewing Window.......................................138

5
8Ordering Information...................................................................141
9Warranty.......................................................................................142

Warning
6
Warning
This device complies with Part 15 of the FCC Rules. Operation is subject to the
following two conditions: (1) this device may not cause harmful interference,
and (2) this device must accept any interference received, including
interference that may cause undesired operation.
NOTE: This equipment has been tested and found to comply with the
limits for a Class B digital device, pursuant to Part 15 of the FCC
Rules. These limits are designed to provide reasonable protection
against harmful interference in a residential installation. This
equipment generates, uses and can radiate radio frequency energy
and, if not installed and used in accordance with the instructions,
may cause harmful interference to radio communications.
However, there is no guarantee that interference will not occur in a
particular installation. If this equipment does cause harmful
interference to radio or television reception, which can be
determined by turning the equipment off and on, the user is
encouraged to try to correct the interference by one or more of the
following measures:
-- Reorient or relocate the receiving antenna.
-- Increase the separation between the equipment and receiver.
-- Connect the equipment into an outlet on a circuit different from that to
which the receiver is connected.
-- Consult the dealer or an experienced radio/TV technician for help.
Notice: (1) Changes or modifications not expressly approved by the
party responsible for could void the use is authority to operate the
equipment.

Warning
7
Major Science SmartView Pro Imager System series has been tested and
found to comply with the limits for the CE regulation. Also, SmartView Pro
Imager System series is RoHS compliant to deliver confident product which
meets the environmental directive. These limits are designed to provide
reasonable protection against harmful interference when the instrument series
is operated in a commercial environment. This instrument series used together
with power supply unit generates, uses, and can radiate radio frequency
energy, and if not installed and used in accordance with the instruction manual,
may cause harmful interference to radio communications. Operation of this
instrument series in a residential area is likely to cause harmful interference in
which case the user will be required to correct the interference at their expense.
Changes or modifications not expressly approved by the party responsible for
compliance could void the user’s authority to operate the equipment. It is
strongly recommended for the user to read the following points carefully before
operating this equipment.
1. Read and follow the manual instructions carefully.
2. Do not alter the equipment. Failure to follow these directions could result
in personal and/or laboratory hazards, as well as invalidate equipment
warranty.
3. Use a properly grounded electrical outlet with correct voltage and current
handling capacity.
4. Disconnect from power supply before maintenance and servicing. Refer
servicing to qualified personnel.
5. Never use this instrument series without having the safety cover correctly
in position.
6. Do not use the unit if there is any sign of damage to the external tank or
cover. Replace damaged parts.
7. Do not use in the presence of flammable or combustible material; fire or
explosion may result. This device contains components which may ignite
such materials.
8. Refer maintenance and servicing to qualified personnel.
9. Ensure that the system is connected to electrical service according to
local and national electrical codes. Failure to make a proper connection
may create fire or shock hazard.
10. Use appropriate materials and operate correctly to avoid possible hazards
of explosion, implosion or release of toxic or flammable gases arising from
overheated materials.
11. The unit shall be operated only by qualified personnel.

Warning
8
Safety Information
Use high level of precaution against any electrical device. Before connecting
the electrical supply, check to see if the supply voltage is within the range
stated at the rating label, and see to it that the device be seated firmly. Place
the unit in a safe and dry location; it must NOT touch the surrounding. Follow
the safety precautions for chemicals / dangerous materials. If needed, please
contact qualified service representative or [e-mail]
Environmental Conditions
Ensure the instrument is installed and operated strictly in the following
conditions:
1. Indoor use only
2. ≤95% RH (non-condensing)
3. 75 kPa – 106 kPa
4. Altitude must not exceed 2000 meters
5. Ambient to 40°C operating temperature
6. Pollution degree: 2
7. Mains supply voltage fluctuations up to ±10% of the normal voltage
Avoiding Electrical Shock
Follow the guidelines below to ensure safe operation of the unit.
SmartView Pro Imager System series has been designed to use with shielded
wires thus minimizing any potential shock hazard to the user. Major Science
recommends against the use of unshielded wires.
To avoid electrical shock:
1. In the event of solution accidentally spilling into the instrument, it must be
dried out for a period of time (at least 2 hours) and restored to NORMAL
CONDITION before each operation.
2. Never connect or disconnect wires loading from the power jacks when the
red indicator light of power switch is on.
3. WAIT at least 5 seconds after stopping a run before handling output leads
or any connected apparatus.
4. ALWAYS make sure that your hands, work area, and instruments are clean
and dry before making any connections or operating the power supply.
5. ONLY connect the power cord to a properly groundedAC outlet.
Avoiding Damage to the Instrument
1.Do not attempt to operate the device if damage is suspected.

Warning
9
2.Protect this unit from physical damage, corrosive agents and extreme
temperatures (direct sunlight, etc.).
3.For proper ventilation and safety concerns, keep at least 10 cm of space
behind the instrument, and at least 5 cm of space on each side.
4.Use high level of precaution against the damages on the unit.
5.Do not operate the unit out of environmental conditions addressed above.
6.Prior to applying any cleaning or decontamination methods other than
manufacturer’s recommendation, users should check with the
manufacturer’s instruction to see if the proposed method will damage the
equipment.
Equipment Operation
Follow the guidelines below to ensure safe operation of the unit:
1. Check the displayed figures to see if the unit is functioning correctly before
using this unit.
2. NEVER access dangerous chemicals or other materials to prevent possible
hazard of explosion and damage.
3. Atemporary conductivity caused by condensation might occur even though
this series is rated Pollution Degree 2 in accordance with IEC 664.
To Disposal UV tubes
The UV tubes contain mercury; please dispose of the tubes in accordance with
local laws. It is important to handle the waste tubes with care to ensure public
health and the environment. For further information about recycle waste tubes,
please see www.lamprecycle.org.
Equipment Handling
Follow the guidelines below to ensure safe move of the device:
1. Carry the device at least two people.
2. Do not over than knuckle height when you moving the device.
3. Move the device by cart and assist the device do not drop.
Symbols
The symbols used on SmartView Pro 2300 Imager System are explained
below.

Warning
10
Indicates an area where a potential shock hazard may exist.
Consult the manual to
avoid possible personal injury or instrument
damage.
Indicate a warning of UV radiation.
While using the UV Transilluminator
, be sure the operating
personnel is properly protected.
Indicates disposal instruction.
DO NOT discards
this unit into a municipal trash bin when this unit
has reached the end of its lifetime. To ens
ure utmost protection of
the global environment and minimize pollution, please recycle this
unit.
Potential Risk and Preventive Measure
1. Risk assessment table
Potential
Risk
Frequency Frequent Likely Possible Rare Unlikely
Bruise 
Pinch 
Slash 
UV radiation
dangerous 
Power cord plug
wrong 

Warning
11
2. Preventative measures of risk
Potential Risk Preventive measures
Bruise Do not put the machine near the table edge.
Move the machine by cart.
Pinch Do not put your hands on the open door.
Slash Prevent hard impact on the acrylic panel.
UV radiation
dangerous Do not open the darkroom door while you turn on the UV light.
Power cord plug
wrong
Observe correct adapter plug.

Introduction
12
1 Introduction
1.1 Overview
SmartView Pro Imager System is the next generation of gel documentation
instrument. It is specifically designed for ease of use for any lab experiment
with gel imaging. SmartView Pro Imager System is packed with 2.0
megapixels high sensitivity and resolution scientific grade camera for
outstanding imaging quality. The system provides you convenience such as
312(302)nm pull out UV transilluminator for easy gel cutting( enclosed with
UVCI-2300; pull out blue transilluminator with UVCI-2400), pre-focused lens
for one touch image capture, and user friendly software interface. The
expendable accessories such as epi-blue module and White Light Plate are
also available for environment safe nucleic acid stains or western blot analysis.
1.2 Feature
- Standalone gel doc system with high-quality monochrome CCD
- 2.0 megapixels CCD resolution
- Intuitive 10.4” interface with color touch-screen
- Zoom-able lens for proper picture size
- Movable platform for gel excision with optional UV-protection shield
- Automatic UV shut off mechanism (UVCI-2300 only)
- Safety door switch
- Optional trans white light and blue light plates (UVCI-2300 only)
- Exclusively designed for EtBr-free operation gel doc system (UVCI-2400
only)
- Easy to use software for image analysis
- Optional trans white light plates

1.3 Components Guide
13
1.3 Components Guide
Rear View Side View
CCD camera and lens
Darkroom door
Touch panel
Viewing window
Power socket & Fuse holder
Power switch
USB port
Front View
Inching switch of the door
(UVCI-2300 only)
Ethernet Port

Specification
14
2 Technical Specification
Model Name UVCI-2300 UVCI-2400
Total Weight
Approx. 32.34 kg
Approx. 25.4 kg
Unit Dimension (W × D × H) 400× 425 × 900 mm 410 × 405 × 915 mm
Display 10" TFT display
Touch Sceen Image capture
Download Images
USB or web
Image Storage Built-in 14G Memory and USB flash
Saved Image Format BMP/JPEG/PNG/TIFF, 8bit ; PNG/TIFF, 16 bit
Interface Ethernet Port 10/100/1000Mb Ethernet port
USB flash drive: Front x 1, Back x 2(all three ports)
Supports Mitsubishi thermal-printer P95DW
Material Painted iron metal , ABS front door
Rated Voltage 100-240V~, 50/60Hz, 2A
Safety Regulation
CE
Stylus Pen Yes
PC Software
Analysis (including process
、
editor
、
analyze
、
summary step)
軟體要綁 License
CCD Camera
Image Sensor 1/1.8" Interline UXGA monochrome
progressive CCD (Sony ICX274AL)
Effective Picture Elements
1628 (H) × 1236 (V)
Resolution 1600 (H) × 1200 (V) (full scanning)
Video Output Format RAW 8 bit / 10 bit / 12 bit
Pixels Size (H × V) 4.4 × 4.4 µm
Max. Frame Rate
15 fps
Interface USB2.0 connector
Grayscale 12 bit, 0 – 4095 gray levels

Specification
15
Manual Zoom Lens
Operation Range Iris Manual control, F1.2-F16C
Operation Range Focus
Manual control, 1m - Inf.
Operation Range Zoom
Manual control, 12.5mm - 75mm
Filter size M55mm
Close-up Lens
+1 close up
Filter (for camera) ★Ordered Separately
Optical EtBr filter
Orange Amber filter
Optical SYBR green filter

Orange Amber filter
Note:
For use with UV light as activation source, optical filters should be used.
For use with blue light as activation source, amber filters should be used.
Darkroom
-
Built-in Drawer Type UV
transilluminator 312(302) nm
-Light source: 8W × 6 tubes
-Built in Blue light Table

Built in LED white light, Light source: 1W x 6 lamps
-UV Safety door switch
N/A
-Maximum field of view 21 × 26cm
-(optional) Blue Light Plate
N/A
-(optional) White Light Plate
Viewing Window
-Built-in UV protection viewing window
-Built-in viewing window amber filter 580nm
PC Minimum Hardware Requirements
Operating Systems Windows® 7 SP1 / Windows® Vista SP1 /
Windows® XP SP3 / Windows® 8/ Windows® 10
Processor 1.8GHz Pentium®
IV or equivalent AMD
Athlon® processor
Storage
1 GB available HD space
Display 1280 × 800 resolutions
Memory 1 GB
Media
CD-ROM drive

3.1-3.2
Installing Optional Light Plate
16
3 Installation Instructions
SmartView Pro Imager System comes with epi white light and trans-UV (UVCI-2300) as
standard. Optional blue light plate or white light plate is offered for expansion to offer higher
flexibility. There are few procedures to install the optional devices:place the unit on a sturdy,
level safe and dry place, then follow the instruction below for installation.
Note: Please skip ahead to section 3.3 if no optional lighting modules are included with
your purchase.
Tool required: A regular screwdriver (not provided)
3.1 Installing Blue Light Plate (Optional)
Step1 Get the Blue Light Plate ready.

3.1-3.2
Installing Optional Light Plate
17
Step2 Open the darkroom door.
*The Blue Light Plate will be installed under the White Light Plate.
Step3 Fasten the Blue Light Plate onto device with a regular screwdriver and four screws.
White Light Plate
Blue Light Plate

3.1-3.2
Installing Optional Light Plate
18
Step4 Connect the Blue Light Plate cable to darkroom inner socket.
NOTICE: Blue Light Plate cable connects to the upper socket.
Finished

3.1-3.2
Installing Optional Light Plate
19
3.2 Installing White Light Plate (Optional)
Step1 Get the White Light Plate ready.
Step2 Open the darkroom door.
Place the White Light Plate as the picture below. Align four screw holes (in yellow circles).
White Light Plate