Major Science SmartView Pro 1100 User manual

SmartView Pro 1100 Imager System
Instruction Manual
Catalog Nos. UVCI-1100
www.majorsci.com
[e-mail]
Version 09H
Revised on : 2019/07/22
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Packing list
Standard Package
SmartView Pro 1100 Imager system
-1 × 5.0MP CMOS camera and lens
-1 × 312(302)nm UV transilluminator
-1 × White light lamps module
-1 × Amber filter viewing window
-1 × Power Cord
-1 × SmartView Pro 1100 Imager System Software CD
-1 × Instruction Manual
Optional Package
Blue Light Module
-2 × Blue lights (left and right)
-2 × M7 nuts
White Light Table Module
-1 × White light table
-2 × M7 nuts
Signed by:
Date:
Major Science is liable for all missing or damaged parts / accessories
within 7 days after customer received this instrument package. Please
contact Major Science immediately regarding this issue. If no response
within such time period from consignee party, that will be consignee
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Packing list ..................................................................................................... 1
Warning ........................................................................................................... 4
Section 1 Introduction ............................................................................ 10
1.1 Overview .............................................................................................. 10
1.2 Feature ................................................................................................. 10
1.3 Components guide ............................................................................... 11
Section 2 Tech nic al S pecif ication .......................................................... 12
Section 3 Installation Instructions ......................................................... 14
3.1 Installing Blue Light Module (Optional) ................................................. 14
3.2 Installing White Light Table Module (Optional)...................................... 18
3.3 Install SmartView Pro 1100 software .................................................... 22
Section 4 Operation Instructions ........................................................... 27
4.1 Capture/Analysis Control interface ....................................................... 27
4.1.1 Capture Screen Interface ........................................................................ 27
4.1.2 Analysis: Image Process Interface .......................................................... 30
4.1.3 Analysis: Image Edit Interface ................................................................. 33
4.1.4 Analysis: Image Analyze Interface .......................................................... 34
4.1.5 Analysis: Image summary Interface ........................................................ 36
4.2 Start the Operation ............................................................................... 37
4.2.1 Positioning your gel ................................................................................ 38
4.2.2 Select the appropriate filter ..................................................................... 38
4.2.3 Turn on the power and connect the system with PC ............................... 39
4.2.4 Adjust the setting for best imaging .......................................................... 40
4.2.5 Select the light source ............................................................................ 41
4.2.6 Adjust the lens Iris ................................................................................... 41
4.2.7 Default setting ......................................................................................... 42
4.2.8 Freeze Image ......................................................................................... 43
4.2.9 Acquire and save the image .................................................................... 44
4.2.10 Print the image ..................................................................................... 45
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................................................................................................................... 46
4.3.1 Load the image ....................................................................................... 46
4.3.2 Processing the Image File ...................................................................... 47
4.3.3 Selecting the Image Lane ....................................................................... 59
4.3.4 Lane Analysis ......................................................................................... 60
4.3.5 Image Summarization ............................................................................. 66
the sample .................................................................................................. 73
4.4.1 Load the image ....................................................................................... 73
4.4.2 Processing the Image File ...................................................................... 74
4.4.3 Selecting the Image Dot.......................................................................... 84
4.4.4 Calculating the density ............................................................................ 86
Section 5 Troubleshooting Guide .......................................................... 88
Section 6 Cleaning & Maintenance ........................................................ 90
6.1 Replacing the Fuse .............................................................................. 90
6.2 Adjust the camera for clearer image ..................................................... 91
6.3 Replacing Amber Filter onto Viewing Window ...................................... 94
6.4 Adjust the scientific camera when out of focus ..................................... 97
Section 7 Ordering Information .............................................................. 99
Section 8 Warranty ................................................................................ 100
Appendix: Install Camera Software .......................................................... 101
I. Install camera software .......................................................................... 101
II. Install light signal software ..................................................................... 108
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Warning
This device complies with Part 15 of the FCC Rules. Operation is subject to the
following two conditions: (1) this device may not cause harmful interference,
and (2) this device must accept any interference received, including
interference that may cause undesired operation.
NOTE: This equipment has been tested and found to comply with the
limits for a Class B digital device, pursuant to Part 15 of the FCC
Rules. These limits are designed to provide reasonable protection
against harmful interference in a residential installation. This
equipment generates, uses and can radiate radio frequency energy
and, if not installed and used in accordance with the instructions,
may cause harmful interference to radio communications.
However, there is no guarantee that interference will not occur in a
particular installation. If this equipment does cause harmful
interference to radio or television reception, which can be
determined by turning the equipment off and on, the user is
encouraged to try to correct the interference by one or more of the
following measures:
-- Reorient or relocate the receiving antenna.
-- Increase the separation between the equipment and receiver.
-- Connect the equipment into an outlet on a circuit different from that to
which the receiver is connected.
-- Consult the dealer or an experienced radio/TV technician for help.
Notice: (1) Changes or modifications not expressly approved by the
party responsible for could void the use is authority to operate the
equipment.
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Major Science SmartView Pro Imager System series has been tested and
found to comply with the limits for the CE regulation. Also, SmartView Pro
Imager System series is RoHS compliant to deliver confident product which
meets the environmental directive. These limits are designed to provide
reasonable protection against harmful interference when the instrument series
is operated in a commercial environment. This instrument series used together
with power supply unit generates, uses, and can radiate radio frequency
energy, and if not installed and used in accordance with the instruction manual,
may cause harmful interference to radio communications. Operation of this
instrument series in a residential area is likely to cause harmful interference in
which case the user will be required to correct the interference at their expense.
Changes or modifications not expressly approved by the party responsible for
complianc
strongly recommended for the user to read the following points carefully before
operating this equipment.
1. Read and follow the manual instructions carefully.
2. Do not alter the equipment. Failure to follow these directions could result
in personal and/or laboratory hazards, as well as invalidate equipment
warranty.
3. Use a properly grounded electrical outlet with correct voltage and current
handling capacity.
4. Disconnect from power supply before maintenance and servicing. Refer
servicing to qualified personnel.
5. Never use this instrument series without having the safety cover correctly
in position.
6. Do not use the unit if there is any sign of damage to the external tank or
cover. Replace damaged parts.
7. Do not use in the presence of flammable or combustible material; fire or
explosion may result. This device contains components which may ignite
such materials.
8. Refer maintenance and servicing to qualified personnel.
9. Ensure that the system is connected to electrical service according to
local and national electrical codes. Failure to make a proper connection
may create fire or shock hazard.
10. Use appropriate materials and operate correctly to avoid possible hazards
of explosion, implosion or release of toxic or flammable gases arising from
overheated materials.
11. The instrument is intended for scientific research use only, and must be
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operated by qualified personnel who realize the potential risks of the use
of this instrument. Major Science makes no claim that its instruments are
designed or certified as medical device; no presentation, promises,
express warranty or implied warranty will be made concerning the
suitability of these instruments for any medical use. Major Science will not
provide customers any notice or certification concerning its products being
compliant as a medical device.
Safety Information
Use high level of precaution against any electrical device. Before connecting
the electrical supply, check to see if the supply voltage is within the range
stated at the rating label, and see to it that the device be seated firmly. Place
the unit in a safe and dry location; it must NOT touch the surrounding. Follow
the safety precautions for chemicals / dangerous materials. If needed, please
contact qualified service representative or [e-mail]
Environmental Conditions
Ensure the instrument is installed and operated strictly in the following
conditions:
1. Indoor use only
2. (non-condensing)
3. 75 kPa 106 kPa
4. Altitude must not exceed 2000 meters
5. Ambient to 40°C operating temperature
6. Pollution degree: 2
7. Mains supply voltage fluctuations up to ±10% of the normal voltage
Avoiding Electrical Shock
Follow the guidelines below to ensure safe operation of the unit.
SmartView Pro Imager System series has been designed to use with shielded
wires thus minimizing any potential shock hazard to the user. Major Science
recommends against the use of unshielded wires.
To avoid electrical shock:
1. In the event of solution accidentally spilling into the instrument, it must be
dried out for a period of time (at least 2 hours) and restored to NORMAL
CONDITION before each operation.
2. Never connect or disconnect wires loading from the power jacks when the
red indicator light of power switch is on.
3. WAIT at least 5 seconds after stopping a run before handling output leads
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or any connected apparatus.
4. ALWAYS make sure that your hands, work area, and instruments are clean
and dry before making any connections or operating the power supply.
5. ONLY connect the power cord to a properly grounded AC outlet.
Avoiding Damage to the Instrument
1. Do not attempt to operate the device if damage is suspected.
2. Protect this unit from physical damage, corrosive agents and extreme
temperatures (direct sunlight, etc.).
3. For proper ventilation and safety concerns, keep at least 10 cm of space
behind the instrument, and at least 5 cm of space on each side.
4. Use high level of precaution against the damages on the unit.
5. Do not operate the unit out of environmental conditions addressed
above.
6. Prior to applying any cleaning or decontamination methods other than
equipment.
Equipment Operation
Follow the guidelines below to ensure safe operation of the unit:
1. Check the displayed figures to see if the unit is functioning correctly before
using this unit.
2. NEVER access dangerous chemicals or other materials to prevent possible
hazard of explosion and damage.
3. A temporary conductivity caused by condensation might occur even though
this series is rated Pollution Degree 2 in accordance with IEC 664.
To Disposal UV tubes
The UV tubes contain mercury; please dispose of the tubes in accordance with
local laws. It is important to handle the waste tubes with care to ensure public
health and the environment. For further information about recycle waste tubes,
please see www.lamprecycle.org.
Equipment Handling
Follow the guidelines below to ensure safe move of the device:
1. Carry the device at least two people.
2. Do not over than knuckle height when you moving the device.
3. Move the device by cart and assist the device do not drop.
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Symbols
The symbols used on SmartView Pro 1100 Imager System are explained
below.
Indicates an area where a potential shock hazard may exist.
Consult the manual to
avoid possible personal injury or instrument
damage.
Indicate a warning of UV radiation.
While using the UV Transilluminator
, be sure the operating
personnel is properly protected.
Indicates disposal instruction.
DO NOT
throw this unit into a municipal trash bin when this unit
has reached the end of its lifetime. To ensure utmost protection of
the global environment and minimize pollution, please r
ecycle this
unit.
Potential Risk and Preventive Measure
1. Risk assessment table
Potential
Risk
Frequency
Frequent Likely Possible Rare Unlikely
Bruise
Pinch
Slash
UV radiation
dangerous
Power cord plug
wrong
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2. Preventative measures of risk
Potential Risk Preventive measures
Bruise Do not put the machine near the table edge.
Move the machine by cart.
Pinch Do not put your hands on the open door.
Slash Prevent hard impact on the acrylic panel.
UV radiation
dangerous
Do not open the darkroom door while you turn on the
UV light.
Power cord plug
wrong Observe correct adapter plug.
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Section 1 Introduction
1.1 Overview
SmartView Pro Imager System is the next generation of gel documentation
instrument. It is specifically designed for ease of use for any lab experiment
with gel imaging. SmartView Pro Imager System is packed with 5.0
megapixels high sensitivity and resolution camera for outstanding imaging
quality. The system provides you convenience such as 312(302)nm pull out
UV transilluminator for easy gel cutting, pre-focused lens for one touch image
capture, user friendly software interface, and the innovative iPad/PC remote
control(1200 series only). The expendable accessories such as epi-blue
module and white light table are also available for environment safe nucleic
acid stains or western blot analysis.
1.2 Feature
- Fluorescent imaging
- 5.0 megapixels CMOS camera
- Mounted USB port for saving images and analyzing samples
- Maximum field of view 21 x 26 cm (8.3 x 10.3 inch)
- Compact configuration and size
- Inner 6 x 1W white light LED for uniform white light illumination
- Safety door switch
- Two kinds of light sources for optional (safety blue light and whit light table)
- High camera resolution
- Easy to use software for image analysis
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1.3 Components guide
Front View
Rear View Side View
Camera and lens
Darkroom door with
UV safety switch
LED indicator light
Viewing window
Power socket
& Fuse holder
Main power
A-type USB
port
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Section 2 Technical Specification
Model Name UVCI-1100
Total Weight Approx. 24.5 kg
Unit Dimension (W × D × H) 410 × 405 × 570mm
Construction Material Painted iron metal, ABS front door
Display LED indicator light
Rated Voltage 100 240V~, 50/60Hz, 2A
Power 120W
Connectivity USB
Image Storage PC
Save Image Format BMP/JPEG/PNG/TIFF, 8 bit; PNG/TIFF, 16 bit
Safety Regulation CE FCC
CMOS Camera
Camera Type CMOS camera
Image Sensor 5M Pixel Monochrome sensor
Resolution (H × V) 2592 × 1944 pixels
Video Output Format RAW 8 bit / 10 bit / 12 bit
Cell Size (H × V) 2.2 × 2.2 µm
Max. Frame Rate 14fps
Interface USB3.0
Dynamic Range 70.1DB
Grayscale 12 bit, 0 4095 gray levels
Lens
Focal Length 6mm
Aperture F1.2
Filter (for camera)
Ordered Separately
Optical EtBr filter / Optical SYBR Green filter /
Ye ll ow A mb er f il te r / Or a nge A mbe r f i l te r
Darkroom
Built-in Drawer Type UV transilluminator 312(302) nm,
Light source: 8W × 6 tubes
Built-in LED white light, Light source: 1W × 6 lamps
UV safety door switch
Maximum field of view 21 × 26cm
(optional) Blue Light Module
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(optional) White Light Table
Viewing Window
Built-in UV viewing window
Built-in viewing window Amber filter (560nm)
PC Minimum Hardware Requirements
Processor
1.8GHz Pentium® IV or equivalent AMD Athlon®
processor
Memory 1GB
Storage 1GB available HD space
Media CD-ROM drive
Connectivity 1 port USB 2.0
Display 1280 × 800 resolutions
Operating System Windows® 7 SP1/ Windows® Vista SP1 /
Windows® XP SP3
/ Windows
®
8
/
Windows
®
10
PC Software
Control UVCI-1100
Image capture
Image processing and gel analysis
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Section 3 Installation Instructions
SmartView Pro Imager System comes with epi white light and trans-UV as
standard. Optional blue light module or white light table is offered for
expansion to offer higher flexibility. There are few procedures to install the
optional devices: place the unit on a sturdy, level safe and dry place, then
follow the instruction below for installation.
Note: Please skip ahead to section 3.3 if no optional lighting modules are
included with your purchase.
Tool Required: 7M/M Socket wrench (not provided)
3.1 Installing Blue Light Module (Optional)
Step1 Get the blue light module ready. There are two blue lights (left and right)
and 2 pieces of M7 nuts in the package.
M7 nuts
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Step2 Open the darkroom door and hold the left blue light on left inner wall.
Step3 Connect the blue light quick connector with darkroom inner socket.
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Step4 Insert the blue light into the inner lining of darkroom.
Step5 Put the other side of blue light into the stud of darkroom inner wall.
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Step6 Take a nu t from p arts kit. Use 7M/M socket wrench to tighten the nut on
the stud.
Step7 The blue light is fixed as shown below. Please repeat steps 2~6 for right
side blue light to install it in the darkroom.
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3.2Installing White Light Table Module (Optional)
Step1 Get the white light table module ready. There are one white light table
and 2 pieces of M7 nuts in the package.
Step2 Open the darkroom door and place the white light table on the UV
transilluminator. There are two studs and a socket on the back wall.
M7 nuts
Before assembling, please check to make sure the
screw studs on both sides of the white light table are
in the upright position.
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Step3 Connect the white light table quick connector with darkroom inner
socket
Step4 Make sure the stud holders on the white light table align with the studs
of darkroom.
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