Navitar ZFL User manual

Fluorescence Imaging
• Utilizes interchangeable professional fluorescence cubes
to create images compatible with most existing camera
systems.
• Much lower cost and smaller (more compact) than a tradi-
tional microscope.
• Field of view up to 9.2 mm with a 2/3” CCD in macro
mode.
• Micro mode utilizes professional infinity corrected objec-
tives.
• Remote UV light source includes a long-life metal halide
bulb.
• Zoom and fixed systems available. Fine focus included
with both systems.
Fluorescence Imaging

Fluorescence Imaging
106 http://machinevision.navitar.com • email: [email protected]
ZFL Video Scope
• Transgenic organisms.
• All GFP (Green Fluorescent Protein) stud-
ies involving living cells and tissues: C.
Elegans, D. Melanogaster, D. Rerio Larva,
Zebra Fish, Oocytes, Nematodes, Droso-
philia.
• Plant cells, plant surfaces, soil samples,
parasites.
• Air and water pollution.
• Forensic - fingerprints, fibers, documents,
and body fluids.
• Capillary flow.
• Bacteria on agricultural products.
• Art restoration.
• Other non-destructive testing.
• Wafer Contamination by organic residue.
• Inspection of photoresist.
• Diagnosis of lumber diseases.
• Inclusions, imperfections, and compound
variation in geological crystals (including
the gem industry).
• Inspection of the structure and chemical
composition of concrete.
• Investigation of the presence and disper-
sions of additives or impurities in polymers
and ceramics.
• Investigation of structural abnormalities in
materials (cracks, pores, welds).
• Confirming presence of adhesives in
cemented applications.
Ideal Uses
Navitar Video Fluorescent Scope
Navitar’s Video ZFL Scope is a Macro/Micro
fluorescent vision system that utilizes inter-
changeable professional fluorescent cubes
and internal focus to create an image compat-
ible with most existing camera systems. It is
a simple means of doing very sophisticated,
task oriented fluorescence without the ex-
pense and complexity associated with a fully
loaded research microscope.
Basic Components of Any Video
Fluorescent System
1. A light source emitting the wavelengths
required to cause the labeling dye to
fluoresce.
Two different remote light sources are
available, a halogen light for the longer
wavelengths, and a metal arc lamp for the
UV. LED illumination is currently under
development.
2. An integrated cube that optimizes per-
formance by stopping all but the desired
(excitation) wavelength from reaching
the object and then stopping all but the
fluorescing wavelength (emitting) from
reaching the camera. There is a multitude
of off-the-shelf cubes available depending

107
Fluorescence Imaging
Phone: [phone] or 585-359-4000 •Fax [phone]
Cellulose Fiber Images in
Macro and Micro Modes
*Magnifications are at the image plane.
Macro Mode
0.5X Lens Attachment
Magnification*=0.9X, W.D.=175
Micro Mode
4X Objective
Magnification*=3.6X
Micro Mode
20X Objective
Magnification*=18X
on which labeling dye is
being used.
The Navitar system
permits the usage of all
standard Olympus BX2
Fluorescent Cubes, which
are available from mul-
tiple sources. These are
captured singularly in
a quick change holder
requiring only a minute to
interchange. Information on filter sets and cubes can be found
at www.chroma.com.
3. A camera whose sensitivity and bandwidth are adequate to
handle the fluorescing light levels (which can sometimes be
minimal).
All other components are optics and mechanics that permit varia-
tion of the magnification (field of view) and resolution, along
with a means of achieving critical focus.
The Navitar system is suitable for operation in either Macro or
Micro mode.
Macro Mode
In the Macro mode, a fixed lens system provides up to a 9.2 mm
diameter field of view at a 165 mm working distance with a 2/3”
format camera. A 6.5X zoom accessory provides up to a 15 mm
diameter field at a 108 mm working distance. Various lens at-
tachments allow the working distance to change from 32 to 165
mm. An add-on focus accessory provides a means of adjusting
focus without requiring a microscope type stand.
Micro Mode
In the Micro mode, the same single lens system provides a 0.9X
coupling of any standard 200 mm tube length, infinity corrected,
fluorescent microscope objective to the camera.
Who is Using the Navitar Video ZFL Scope?
• OEM customers creating an automated system for scanning
and post processing of proteomics.
• Production laboratory using image processing software to
count and categorize selected images.
• High school or university professor, on a limited budget, who
needs to demonstrate certain fluorescent characteristics to a
group.
• A field operator who needs instant results involving agriculture,
oceanography, or criminology.
• Museum or aquarium setting-up a public display.
• Research scientists in government laboratories.

Fluorescence Imaging
108 http://machinevision.navitar.com • email: [email protected]
ZFL System Diagram
Camera
(Not Supplied)
Lens Attachments
Macro Mode
Lens
Micro Mode
Objective Adapters
Lab Grade Microscope
Infinity Corrected Objectives
(Not Supplied)
12mm
Fine Focusing Adapter
8-16383
Olympus BX2 Mounted Filter Set
Endow GFP Longpass Emission
(Visit www.chroma.com for
other filter options
)
1-6010
C-Mount Couple r
1-61400
1X Mini Adapter
1-60349
6.5X UltraZoom
non F.F.
1-15801
0.9X Straight Coupler
2-14048
Stand
1-15800
Cube Mounting Block
2-16265
12mm Fine Focus
Assembly 2-50145
1.0X Con verter Lens
1-50012
0.5X
1-50013
0.75X
1-50014
1.5 X
1-50015
2X
2-50025
M26 x 36T
2-50026
0.800 x 36T
RM S
2-51370
M25 x 0. 75T
3-15818
Mounting Block Support
1-17931
Beam Attenuator
3-15807*
Guide Bushing
8-15820
Liquid Li ght Guid e
1 Meter Long
* Included w/ 1-15800
** T= Tele
Light Source
8-61172
120V Fiber Optic Power Supply
(150w EKE lamp,0.720" fiber receptacle)
8-15799
UV Light Source
For Use With Liquid Light Guide
1- 60162
Flexible Light Pipe for Coax
0.718" input ferrule, 3 foot length
3-17185*
Guide Bushing
1-6218
1X Adapter T**
1-6030
2X Adap ter
1-62922
2X F-Mount Adapter
OR
Rev. 031708
1-6270
Mounting Clamp