BestScope BS-2082 Series User manual

Version:V1.0
BS-2082 Series
Biological Microscope
Instruction Manual
This instruction manual is for the operation guide, troubleshooting and
maintenance to the BS-2082 series biological microscope. Please study this
manual thoroughly before operating and keep it with the instrument. The
manufacturer reserves the rights to the modifications by technology
development. On the basis of operation ensured, technical specifications
may be subject to changes without notice.

BestScope International Limited
Contents BS-2082 Series
Before Use
1. Components..................................................................................................................................1
2. Assembling....................................................................................................................................5
2-1 Assembling Scheme ............................................................................................................5
2-2 Assembling Steps ................................................................................................................7
3. Operation....................................................................................................................................10
3-1 Set Illumination.................................................................................................................10
3-2 Place the Specimen Slide ..................................................................................................10
3-3 Select the Light Path .........................................................................................................11
3-4 Adjust Focusing.................................................................................................................11
3-5 Adjust the Focusing Tension .............................................................................................12
3-6 Adjust the Interpupillary Distance ....................................................................................12
3-7 Use the Eye-cap ................................................................................................................12
3-8 Adjust Stage......................................................................................................................12
3-9 Center the Condenser .......................................................................................................14
3-10 Adjust the Field Diaphragm ............................................................................................14
3-11 Adjust the Aperture Diaphragm .....................................................................................15
3-12 Use the Color Filter .........................................................................................................15
3-13 Replace the Fuse.............................................................................................................16
3-14 Assemble and Use the TV Device....................................................................................16
4. Assembling and Operation of Fluorescence Illuminator .............................................................17
4-1 Assemblage Steps .............................................................................................................17
4-2 Operation..........................................................................................................................21
5. Troubleshooting ..........................................................................................................................26
5-1 Solutions of BS-2082 Series Biological Microscope Problem ............................................26
5-2 Solutions of Mercury Bulb Fluorescent Device Problem ...................................................28

BestScope International Limited
Before Use BS-2082 Series
1. Operation Notice
1. As the microscope is a high precision instrument,
always operate it with care, and avoid physical shake
during the operation.
2. Do not expose the microscope in the sun directly,
either not in the high temperature, damp, dust or
acute shake. Make sure the worktable is flat and
horizontal. Following environment is required when
operating: Indoor temperature: 5℃～40℃, Max
relative humidity: 80%.
3. When moving the microscope, use both hands to
hold its back hand-clasping ① and the front base ②,
and lay it down carefully (see Fig. left).
★It will damage the microscope by holding the
stage, focusing knob, head or light source when
moving.
4. When working, the surface of light source will be
very hot. Make sure there is enough room for the
heat dissipating around the light source.
5. Connect the microscope to the ground to avoid
lightning strike.
6. For safety, make sure the power switch is at “O”
(off) and power it off before replacing the bulb or
fuse (see Fig. left).
7. Wide voltage range is supported as 100~240V.
Additional transformer is not necessary. Make sure
the voltage is in this range.
8. Use the special wire supplied by our company.
2. Maintenance
1. Wipe the lens gently with a soft tissue. Carefully wipe off the oil marks and fingerprints on the
lens surfaces with a tissue moistened with a small amount of 3:7 mixture of alcohol and ether or
dimethylbenzene.
★As the alcohol and ether is flammable, don’t place these chemical near to fire or fire source.
For example, when turning on or turning off the electrical device, please use these chemical in
a ventilated place.
2. Don’t use organic solution to wipe the surfaces of the other components. Please use the
neutral detergent if necessary.
3. If the microscope is damped by liquid, please power it off immediately and wipe it dry.
4. Never disassemble the microscope, otherwise the performance will be affected or the
instrument will be damaged.
5. After using, cover the microscope with a dust cover.
3. Safety Sign
Sign
Signification
The surface gets hot and don’t touch it with bare hand.
Study the instructions before use. Unsuitable operation would lead to
person hurt or instrument faulty.
|
Main switch ON
O
Main switch OFF

BestScope International Limited
1
1. Components BS-2082 Series
BS-2082 Series Biological Microscope Components
Objective
Trinocular head
Light Path
Selecting Pole
Inner
Hexagon
Spanner
Body
Voltage
Indicator
Light
Source
Group
Reset Button
For Intensity
Mechanical
Stage
Condenser
Illuminator
Light
Adjustment
Knob
Tension
Adjustment
Knob
Switch Lever
For Filters
Y-axis Moving
Knob
X-axis Moving
Knob

BestScope International Limited
2
BS-2082 Series
Fine
Focusing
Knob
Coarse
Focusing
Knob
Condenser
up-down knob
Dust Cover
Coarse Adjusting
Limit Ring
Nosepiece
Objective

BestScope International Limited
3
BS-2082 Series
BS-2082F Series Biological Fluorescence Microscope Components
Trinocular
head
Flashboard
knob
XY Body
Vertical centering
Screw
Blinkers
Board
Field Diaphragm
Adjusting Pole
Aperture Diaphragm
Adjusting Pole
Lens Focusing Knob
Diaphragm
Centering Screw
Horizontal
centering Screw
Power Supply Box
Vertical
Fluorescence
Illuminator

BestScope International Limited
4
BS-2082 Series
ND Filter Slot

BestScope International Limited
5
2. Assembling BS-2082 Series
2-1 Assembling Scheme
Following is the Assembling Scheme, and the numbers denote the assembling order.
★Before assembling, make sure there is no dust, dirt or other matters to affect the assembly.
Assemble carefully and do not scrap any part or touch the glass surface.
1. BS-2082 Series Biological Microscope Assembling Scheme

BestScope International Limited
6
BS-2082 Series
2. BS-2082 Series Biological Fluorescence Microscope Assembling Scheme

BestScope International Limited
7
BS-2082 Series
2-2 Assembling Steps
2-2-1 Assemble and Replace the Halogen Bulb
(1) Loose lock-screw ① completely by M4 spanner,
then take off the cover group. (See Fig. 1)
(2) Open the bulb lock piece②, and take the bulb③
with clean glove or soft tissue, insert the bulb pins④
into the jack on the bulb holder⑤, the bulb should be
vertical after assembly. (See Fig. 2)
(3) Loose the bulb lock piece②, lock the bulb and
install the cover group.
★Don’t touch the bulb with fingers. If there is a
fingerprint left on the bulb, please wipe it with
clean soft cloth.
★Bulb selected only: 12V/100W Halogen bulb (Philips
7724).
★Before replacing the bulb, make sure to cut off the
main power and wait for both the bulb base and
bulb cooling down.
2-2-2 Assemble the Light Source Group
Push the light source holder① into the body holder,
Make the light source group to be horizontal with the
body group and lock the screw②. (See Fig. 3)
2-2-3 Assemble the Stage
(1) Loosen the lock-screw① on the stage. (See Fig. 4)
(2) From a rear area of the rounded hole center on the
base, carefully ring the two “V” buttons on the bottom
of the stage into the “V” rounded groove②, then
screw down the lock screw①.
Fig.3
Fig.2
Fig.1
Fig.4

BestScope International Limited
8
BS-2082
Series 2-2-4 Assemble the Condenser
(1) Rotate the coarse focusing knob① to raise stage to
the highest. (See Fig. 5)
(2) Rotate the condenser up-down knob② to lower
the condenser bracket to the lowest.
(3) Loosen the condenser lock-screw③ fully.
(4) Swing out the front lens of condenser with the
scale forward. Make the lock screw④of condenser in
alignment with the groove⑤of the condenser stand.
Push the condenser into the innermost of stand.
(5) Screw down the condenser lock-screw③, and raise
the condenser to the highest position with the
condenser up-down knob②.
2-2-5 Assemble the Nosepiece
(1) Loose the lock screw①on the arm. (See Fig. 6)
(2) Match the dovetail interface② of the nosepiece
with the dovetail groove of the arm, and push it to the
bottom. Tighten the lock screw①.
2-2-6 Assemble the Head
(1) Loosen the head lock-screw① fully. (See Fig. 7)
(2) From a little right position, insert the coattail
interface on the bottom of head into the hole of
middle head with a little left inclined. Keep the two
eyepiece tubes forward, and then screw down the lock
screw①.
2-2-7 Assemble the Objective
Rotate the coarse focusing knob to lower the stage.
Install the objectives into the nosepiece from the
lowest magnification to the highest in a clockwise
direction. (See Fig.8)
★Search and focus the sample by low magnification
objective (4X or 10X) when operating. Then get
change to the high magnification ones according to
the observation requirements.
Fig.7
Fig.8
Fig.5
Fig.6

BestScope International Limited
9
BS-2082 Series
★When replacing the objective, rotate the nosepiece
until it sounds “ka-da”, to make sure the objective
is in the center of the light path.
2-2-8 Assemble the Eyepiece
(1) Take down the cover of eyepiece tube① (See Fig.
9).
(2) Insert the eyepiece into the eyepiece tube, until
touch the bottom.
Note: if there is position screw on the eyepiece, match
the position screw② to the groove③ on the
eyepiece, and insert the eyepiece into the eyepiece
tube, until touch the bottom.
2-2-9 Connect Power Cord
(1) Make sure the main switch is at “O” (OFF) position.
(2) Match the gap of the lower light source aviation
plug① to the gap of the aviation socket②, and insert
it to the bottom. (See Fig. 10)
(3) Insert one end of power cord③into the power
socket④of the microscope.
(4) Insert the other end of power cord into the power
supply socket.
★Don’t use strong force when the power cord is
bended or twisted, otherwise it will be damaged.
★Use the special wire supplied by our company. If
it’s lost or damaged, choose one with the same
specifications.
★Connect the power cord appropriately to make
sure the instrument is connected to ground.
Fig.9
Fig.10

BestScope International Limited
10
3. Operation BS-2082 Series
3-1 Set Illumination
(1) Put through the power and turn on the main power
switch to“—” (see Fig. 11).
(2) Adjust the light adjustment knob④until the
illumination is comfortable for observation. Rotate the
light adjustment knob clockwise to raise the voltage
and brightness. Rotate the light adjustment knob
counterclockwise to lower the voltage and brightness.
(3) Press the light intensity reset button①, reset the
light intensity to preset position. Rotate the setting
screw② with a small “-” type screwdriver to set the
light intensity. Rotate it in clockwise to raise the light
intensity, while lower it in counterclockwise.
★The number on the right on Voltage Indicator③
show the voltage.
★Use of bulbs in the low-voltage state can extend
the bulb life.
★When the light intensity reset button is pressed,
rotate the light adjustment knob does not work.
★The light intensity of this machine is preseted to
the best for photomicrography by LBD filter. (About
8V, at location of sign )
3-2 Place the Specimen Slide
(1) Push the wrench① of the specimen holder
backwards (see Fig. 12).
(2) Loosen the wrench①, and clamp the slide② by
the clips while the cover glass faces up.
(3) Rotate the X-axis knob④ and Y-axis knob③. Move
the specimen to the center (alignment with the center
of the objective).
Fig.11
Fig.12

BestScope International Limited
11
BS-2082 Series
3-3 Select the Light Path
For trinocular head, the light path selecting pole①
control the light-energy ratio of binocular and
trinocular. When the light path selecting pole is pushed
to the innermost, all the light will enter the binocular
head. When the light path selecting pole is pulled to
the middle position, binocular and trinocular can be
observed simultaneously. When it is pulled to the
outmost, all the light will enter the trinocular head,
which can only used for trinocular observation
(TV&Photography). (See Fig. 13)
3-4 Adjust Focusing
(1) Put the slice on the stage, and hold it down with
the clip. Shift the 4X objective into the light path. (See
Fig. 14)
(2) Loosen the random upper limit knob①, then
observe the right eyepiece with the right eye. Rotate
the coarse focusing knob② until the image appears in
the view field, then lock the random upper limit
knob①.
★The random upper limit knob can prevent the
objective touching the slice when focusing.
★The random upper limit knob does not react
on the fine focusing knob.
(3) Rotate the fine focusing knob③ for clear details.
★When observing with the 4X or 10X objective, open
both the aperture diaphragm and field diaphragm
to the maximum position, and swing out the front
condenser lens. See “3-9 Center the Condenser” for
condenser operations.
Fig.14
Fig.13

BestScope International Limited
12
BS-2082 Series
3-5 Adjust the Focusing Tension
If the handle is very heavy when focusing or the
specimen leaves the focus plane after focusing, or the
stage declines itself, please rotate the tension
adjustment knob① according to the arrowhead
pointed direction. (See Fig. 15)
3-6 Adjust the Interpupillary Distance
When using two eyes to observe, hold the bases of the
prism and rotate them around the axis to adjust the
interpupillary distance, until there is only one field of
view. (See Fig. 16)
The dot “·” on the left eyepiece base points to the
scale of the interpupillary distance indicator. The scale
value is the interpupillary distance.
Adjustable range: 50~76mm.
★Remember your eye’s interpupillary distance, so
that you can use it next time.
3-7 Use the Eye-cap
(1) Turn over the eye-cap if the user is wearing glasses,
so that it can prevent the glasses touching the
eyepieces and avoid damaging to both glasses and
eyepieces.
(2) Open the eye-cap if the user doesn’t wear glasses,
so that it can prevent stray light disturbing the
observation. (See Fig. 17)
3-8 Adjust Stage
When observation, move the stage by rotating the
x-axis adjustment knob① and the y-axis adjustment
knob② (See Fig. 18) . The moving range of x-axis and
y-axis is 80x55mm.
Adjust the x-axis and y-axis knob:
Fig.15
Fig.16
Fig.17

BestScope International Limited
13
BS-2082 Series
(1) Hold the x-axis knob①, tighten up the y-axis
knob②, to expose the adjustment knob.
(2) Rotate the x-axis knob③ or the y-axis knob④ in
clockwise (the direction of the arrow), to reduce
tension, while increase tension in counterclockwise.
★If the tension is too tight, a creak sound will be
heard when landing the stage; or the accuracy of the
stage stop will be reduced.
Caution: After long time use, the stage rail may be
offset, and the moving range will be shorten.
However, it is not a fault and can be easily regulated
in the following method.
[Process]
Horizontal: Hold the sample holder, and move left and
right the stage rail, hit the limit stopper.
Vertical: Hold the top surface of the stage and move
back and forth, hit the limit stopper.
◎For reflected illumination
Lower the stage bracket, the microscope can adapt the
height of sample no more than 35mm, which is very
useful when observing metallurgical samples and other
thick objects.
(1) Move the stage to the lowest, and then remove the
stage from the microscope.
(2) Loosen the stage bracket lock screw①, and then
remove the stage bracket. (See Fig. 19)
(3) Rotate the coarse focusing knob, and rise up the
focus skateboard② to the position where the limit
screw③ can be seen from the mirror arm. (See Fig.
20)
(4) Loosen and remove the limit screw③.
(5) Reinstall the stage bracket and the stage.
Fig. 18
Fig. 20
Fig. 19

BestScope International Limited
14
BS-2082 Series
3-9 Center the Condenser
(1) Rotate the condenser up-down knob① to raise it
to the highest position. (See Fig. 21)
(2) Rotate the spanner② to move the front lens into
light path.
★Move the front lens of condenser into light path
when the objective is beyond 20X.
(3) Move the 20X objective into light path and focus
the specimen.
(4) Rotate the field diaphragm adjustment ring③ to
put the field diaphragm to the smallest position, then
the image of field diaphragm can be observed through
eyepiece.
(5) Rotate the condenser up-down knob to adjust the
image to the clearest.
(6) Adjust the condenser center adjusting screw④ to
put the image to the center of the field of view.
(7) Open the field diaphragm gradually. If the image is
in the center all the time and inscribed to the field of
view, it shows condenser has been centered correctly.
(See Fig. 22)
(8) In actual use, you can enlarge the field diaphragm a
bit and make the image circumscribed to the field of
view.
3-10 Adjust the Field Diaphragm
By limiting the diameter of light entering the
condenser, the field diaphragm can prevent other light
and strengthen the image contrast. When the image is
just on the edge of the field of view, the objective can
perform best and obtain the clearest image.
Rotate the field diaphragm adjustment ring③ in
clockwise, to enlarge the field diaphragm; otherwise,
to decrease it in counterclockwise. (See Fig. 21)
Fig.22
图17
Fig.21
Fig.23

BestScope International Limited
15
BS-2082 Series
3-11 Adjust the Aperture Diaphragm
The aperture diaphragm decides the numerical
aperture of the illumination system. If the N.A. of
illumination system matches with the N.A. of the
objective, it can obtain better resolution and contrast,
and increase the depth of field.
Adjust the aperture diaphragm adjusting ring③ to
control the size of diaphragm. Take off the eyepiece if
necessary, observe from the eyepiece tube and adjust
the aperture diaphragm adjusting ring③ until see the
figure as shown in Fig. 23 (see Fig. 23&24).
Use of scale: set the scale of condenser N.A. to the
80% value of objective④ N.A (see Fig.24).
In actual use, adjust the aperture diaphragm according
to the size of the sample image contrast, until it’s
comfortable for observation and the contrast is good.
3-12 Use the Color Filter
The color filter can make the background light more
suitable and strengthen the image contrast.
When an external color filter is used, put a diameter
45mm filter into the groove of the condenser
base①(See Fig. 25).
★There are four colors of filter selectable: blue, green,
yellow and white.
★Place the rough side of filter downwards.
When an internal color filter is used, pull the
pole②—⑤ to the outmost, the filter will be moved
into the light path. Otherwise, push the pole into the
innermost, the filter will be moved out of the light path.
(See Fig. 25)
Fig. 24
Fig. 25

BestScope International Limited
16
BS-2082 Series
Filter type/function
②
ND6 (Neutral density filters, used for light
intensity adjustment, which transmission is 6%）
③
ND25 (Neutral density filters, used for light
intensity adjustment, which transmission is
25%）
④
LBD (Chroma balance, daylight-type color filters)
⑤
OP (Optional) Filter base
3-13 Replace the Fuse
(1) Before replace fuse, please set the main power
supply at “O” (OFF) and take off the plug.
(2) Fasten the flute ① under the fuse holder ② by
fingers, take out the fuse holder ② from socket ⑤.
Then take off the fuse ④ from the above flute ③ and
replace it with a new one. Then put it to the flute ③
and push the fuse holder ② into the socket ⑤, until
a sound of “kada” is heard. (See Fig. 26)
★Specifications of the fuse: 250V, 3.15A.
3-14 Assemble and Use the TV Device
(1) Loosen the lock screw① of trinocular head, and
take out the dust-cover②(See Fig.27).
(2) Take down the dust-cover of the TV adapter③.
Insert the TV adapter into the trinocular head as
shown in the figure and screw down the lock screw①.
(3) Screw CCD or video camera into CTV, make sure it is
locking.
(4) For binocular observation, pull the light path
selecting pole④ to the outmost and observe the
image. If the image is unclear, rotate the adjustment
crew⑤ until it is clear.
Fig.27
Fig. 26

BestScope International Limited
17
4. Assembling & Operation of Fluorescence Illuminator BS-2082 Series
4-1 Assemblage Steps
4-1-1 Assemble the Fluorescence Illuminator
(1) Use a tool with cuspidal end to plug into gaps of the
caps① on the illuminator, take off the caps. (Fig. 28)
(2) Put the illuminator into the body along with the
rounded side, then push it toward the light source
direction, make the epi-illuminator close to the body.
(3) Tighten the 4pcs M5 hexagon screws in the
illuminator with the spanner, then cover back the cap.
4-1-2 Assemble the Fluorescence Filter Sets
(1) Loose the right side lock screw ① of the turntable
fluorescence illuminator, with a M4 inner hexagon
spanner, and pull the front cover group ② out of the
dovetail groove. (See Fig. 29)
(2) The blinker board ⑤ is installed in the
fluorescence filter group. When using the fluorescence
filter group, first loose the lock screw ⑥ with the
inner hexagon spanner, and take off the blinker board
⑤. (See Fig. 30)
(3) Put the diaphragm slice of the fluorescence filter
group ⑦ which is to be assembled upward, and match
the dovetail groove of the fluorescence filter group ⑦
with the dovetail wedge of the front cover group ②,
and push to the bottom. Tighten the lock screw ⑥.
(4) Check the ID ⑧ on the dovetail interface, and
insert the nameplate ④ of the fluorescence filter
group into the interface ③ with the same number in
front of the front cover group ②.
(5) Repeat the steps above, to assemble other
fluorescence filter groups into the turntable of the
front cover group ②.
(6) Then match the dovetail wedge of the front cover
group ② with the dovetail groove of the turntable
epi-illuminator, and push to the bottom. Tighten the
lock screw ①.
Fig.30
Fig.29
Fig. 28