NeoBiotech N10 Series User manual

Operation Manual
NB-12-0006
Dry Bath Incubator
N10 Series

Foreword
Thank you for purchasing our product: Dry Bath. This manual for users
contains function and operation of the instrument. In order to use the
instrument properly, please read this manual carefully before using the
instrument.
Opening Check
Please check the instrument and Appendix with the packing list when you
first open the instrument packing case. If you find there is something wrong
with the instrument and the Appendix, do contact the vendor or the
producer.
Document NO.：AS93SM
Document Version：1st Edition, May. 2012

Safety Warnings and Guidelines
1. Important operation information of the security:
Before the users’ operation, they should have a perfect conception of
how to use the Instrument. Therefore, read this Manual carefully before
using it.
2. Security:
The operation, maintenance and repair of the instrument should comply
with the basic guidelines and the remarked warning below. If you don’t
comply with them, it will have effect on the scheduled using life of the
Instrument and the protection provided.
Operation before reading the manual is forbidden. Read the guidelines
and directions below and carry out the countermeasure according to
them.
This product is a normal and an indoor Instrument which conforms to
Standard B style- I type- GB9706.1.
Before using the device, read the manual carefully. These units are
designed for use in laboratory environments. The device must be used
by skilled personnel with the appropriate training.
The operator should not open or repair the Instrument by himself, which
will result in losing the qualification of repair guarantee or occur
accident. If there is some wrong with the Instrument, the company will
repair it.
A.C. power’s grounding should be reliable to safeguard against an
electric shock. The 3-pin plug supplied with thermo-shaker’s power cable
is a safety device that should be matched with a suitable grounded
socket.
Before power on, guarantee the voltage used should be accordant to the
voltage needed, and the rated load of electrical outlet should not lower
than the demand.
If the electric line is damaged, the user should replace it with the same
type. The user should assure there’s nothing on the electric line and the
user should not put the electric line in the ambulatory place.
Hold the jack when the user pull out the electric line, and don’t pull the
electric line.

3. The maintenance of instrument
The wells on the block should be regularly cleaned by soft clean cloth stained with
a little alcohol, to ensure the tubes fully contact with tubes, and has good thermal
conductivity without pollution.
If there are smutches on the instrument, clean them by soft cloth stained with
cleaning cream.
The Instrument should be put in the place of low temperature, little dust,
no water and no sun or strong lamp. What’s more, the place should be
good aeration, no corrosively gas or strong disturbing magnetic field, far
away from central heating, camp stove and other hot resource. Don't put
the Instrument in wet and dusty place. The vent on the Instrument is
designed for aeration. Don’t wall up or cover the vent in order to keep from
high temperature. If the user uses the more than one Instrument the same
time, the distance between them should be more than 100cm.
Power off when the user finishes the work. Pull off the connector plug
when there’s long time no use of the Instrument and cover it with a cloth or
plastic paper to prevent from dust.
Power off when cleaning the instrument.
When cleaning the well, don’t drop the cleaning liquid in the well.
Corrosive cleaning liquid is strongly prohibited.
Pull the connector plug from the jack at once in the following cases, and
contact the vendor:
There is some liquid flowing into the instrument;
Drenched or fire burned.
Abnormal operation: such as abnormal sound or smell.
Instrument dropping or outer shell damaged.
The function has obviously changed.

Contents
CHAPTER 1 INTRODUCTION--------------------------------------------------------------------------------- 1
CHAPTER 2 SPECIFICATIONS------------------------------------------------------------------------------- 2
1. THE NORMAL OPERATING CONDITION: ---------------------------------------------------------- 2
2. THE BASIC PARAMETERS AND CHARACTERISTICS----------------------------------------- 2
3. OPTIONAL BLOCK----------------------------------------------------------------------------------------- 3
CHAPTER 3 BASIC INSTRUCTIONS----------------------------------------------------------------------- 4
1. STRUCTURE OVERVIEW-------------------------------------------------------------------------------- 4
2. KEYPADS ----------------------------------------------------------------------------------------------------- 5
3. DISPLAY------------------------------------------------------------------------------------------------------- 5
CHAPTER 4 OPERATION GUIDE---------------------------------------------------------------------------- 6
1. TEMPERATURE AND TIME SETTING---------------------------------------------------------------- 6
2. RUNNING/STOP--------------------------------------------------------------------------------------------- 6
3. TEMPERATURE CALIBRATION------------------------------------------------------------------------ 7
4．TEMPERATURE CONTROL BY EXTERNAL SENSOR------------------------------------------11
5．THE EXCHANGE OF METAL BLOCK ----------------------------------------------------------------11
CHAPTER 5 FAILURE ANALYSIS AND TROUBLESHOOTING -----------------------------------13
ANNEX：WIRING DIAGRAM---------------------------------------------------------------------------------14

Dry Bath N10 Series Operation manual Chapter 1 Introduction
─1─
Chapter 1 Introduction
The dry bath is a new product controlled by the miniature computer,
which can be widely used for the preservation and reaction of the
samples, the amplification of DNA, the pre-denaturation of the
electrophoresis and blood serum coagulation.
Features of this product are as follows:
Temperature and time digital displayed in LED;
Display of setting temperature and the count down time to the constant
temperature;
Using metal blocks to avoid sample pollution;
Easy replacement, cleaning and disinfecting of metal blocks;
Built-in over-temperature protection, more reliable for using;
Calibrating temperature discrepancy;
Buzzer alarm at the end of temperature control duration.

Dry Bath N10 Series Operation manual Chapter 2 Specifications
—2 —
Chapter 2 Specifications
1. The normal operating condition:
Ambient temperature：5C 35C
Relative humidity：≤70%
Power supply：AC220V～50-60Hz
2. The basic parameters and characteristics
Basic parameters：
Type
Parameter
NB-12-0006A
NB-12-0006B
NB-12-0006C
Temperature range
RT+5C 105C
Timing time
Max. 99h59min.
Temperature control
accuracy
≤ 0.5 C
Display accuracy
0.1 C
Temperature uniformity
≤ 0.5 C
Heating time（20-105C）
≤20 min
Block
1pc
2pcs
Power
120W
240W
Fuse
250V 1.5A Ф5×20
250V 3A Ф5×20
Meas.(mm)(L×W×H)
250x190x130
365x210x150
Weight（kg）
2.5
4.5

Dry Bath N10 Series Operation manual Chapter 2 Specifications
—3 —
3. Optional block
Item No.
Capacity + Diameter
Meas.(mm)
NB-12-0006-01
96x0.2ml or 96 PCR plate
108 x 72 x 60
NB-12-0006-02
45x0.5ml Falcon
108 x 72 x 49
NB-12-0006-03
35x1.5ml Falcon
108 x 72 x 49
NB-12-0006-04
35x2.0ml Falcon
108 x 72 x 49
NB-12-0006-05
15x0.5ml + 20x1.5ml Falcon
108 x 72 x 49
NB-12-0006-06
20x1.5ml + 15x2.0ml Falcon
108 x 72 x 49
NB-12-0006-07
32x0.2ml + 22x0.5ml +
9x1.5ml Falcon
108 x 72 x 49
NB-12-0006-08
20x5ml Falcon
108 x 72 x 49
NB-12-0006-09
20x10ml Falcon
108 x 72 x 49
NB-12-0006-10
12x15ml Falcon
108 x 72 x 60
NB-12-0006-11
6x50ml Falcon
108 x 72 x 60
NB-12-0006-12
96 wells microplate
116.5 x 75 x 60
NB-12-0006-13
Plate
108 x 72 x 60
NB-12-0006-14
384 PCR plate
108 x 72 x 60
NB-12-0006-15
40x6mm tube
108 x 72 x 49
NB-12-0006-16
28x10mm tube
108 x 72 x 49
NB-12-0006-17
24x12mm tube
108 x 72 x 49
NB-12-0006-18
24x13mm tube
108 x 72 x 49
NB-12-0006-19
14x15mm tube
108 x 72 x 49
NB-12-0006-20
14x16mm tube
108 x 72 x 49
NB-12-0006-21
12x19mm tube
108 x 72 x 49
NB-12-0006-22
11x20mm tube
108 x 72 x 49
NB-12-0006-23
6x26mm tube
108 x 72 x 49
NB-12-0006-24
6x28mm tube
108 x 72 x 49
NB-12-0006-25
2x40mm tube
108 x 72 x 49
NB-12-0006-26
2x7 (Cuvette)
108 x 72 x 49
NB-12-0006-27
PT1000
NB-12-0006-28
M4

Dry Bath N10 Series Operation manual Chapter 3 Basic Instructions
—4 —
Chapter 3 Basic Instructions
This chapter focuses on the introductions of the structure, keypads and key-functions of
the instrument, as well as preparatory work before starting. Please read this chapter
before starting when uses this instrument for the first time.
1. Structure overview
Block
Keypad
Power supply
plug
Fuse
On/Off button
Transparent cover

Dry Bath N10 Series Operation manual Chapter 3 Basic Instructions
—5 —
2. Keypads
3. Display


Start
Stop
For running, timing begins.
Drop key: For number decreasing.
For stopping, timing ends.
Up key: For number increasing.

Dry Bath N10 Series Operation manual Chapter 4 Operation Guide
—6 —
Chapter 4 Operation Guide
1. Temperature and time setting
a) Press the On/Off button; the display flashes 3
times, the instrument goes into the initial
state with the sound of “beep”.
b) After about 3 seconds, the temperature
automatically goes up to the setting
temperature, the 28.5 in display is the
real-time temperature of the block (It
indicates the temperature now is 28.5°C). The
00:35 in display is the running time set
previously.(It indicates the running time is 35
min).
c) Press or in Temp to modify the
temperature.
d) Then press or at Time to modify the
time.
Wait 6 seconds to confirm the input temperature and time, and they flash
simultaneously and disappear, then the temperature automatically goes up to the
setting temperature.
2. Running/Stop
a) After the temperature and time setting
completed, the instrument automatically goes
up to the setting temperature, press “Start”
key and immediately release, then the

Dry Bath N10 Series Operation manual Chapter 4 Operation Guide
—7 —
thermostat countdown begins with the sound of "beep".
Tips：Decimal point regularly flashing, which
indicatest the instrument still in the status
of temperature control, timing has not yet
begin.
Decimal point kept stationary, which
Indicates that the instrument has had constant
temperature already, the timing begins.
When timing ends, running stopped and the
buzzer alarms. Now the temperature remains
setting temperature, and displays “”.
It indicates that running is over.
b) After running ends, press any key return.
c) When running, press ”Stop”key to stop.
3. Temperature Calibration
The temperature of the instrument has been calibrated before it is sent out. But if there
is deviation between the actual temperature and the displayed temperature due to
some reasons, you can do as follows to correct the error.
Caution: the instrument uses double temperatures adjustment to ensure its veracity.
This means that it is linearly adjusted on 40℃and 100℃two points. The
temperature veracity will be within ±0.5℃after the double temperatures
adjustment.
Both the circumstance temperature and the block temperature should be lower than
35℃.
3.1. Calibrated with a thermometer, specific methods are as follows：
a) After the startup of the instrument, it enters into waiting interface. Make sure that the

Dry Bath N10 Series Operation manual Chapter 4 Operation Guide
—8 —
temperature in display is below 35℃. If the temperature is higher than 35℃, you
should wait until the temperature is below 35℃.
b) Inject olefin oil into one of the cone-shaped wells, and then put a thermometer into
this well (make sure that the precision of the thermometer should be within 0.1℃
and the temperature ball should be absolutely immerged into the cone-shaped well).
See Fig a.
c) Press “Stop” key without releasing, then press 
key of temperature at the same time for 3 seconds,
it will enter into temperature calibration interface,
see the right. At this point, “”displayed, which indicates entering into
temperature calibration program. The temperature displayed is current
temperature and begins to rise to 40.0℃automatically.
When the temperature reaches to 40℃constant
temperature, the decimal digit begins to flicker,
waiting for the calibrated value of 40℃. Read out the
actual value from the thermometer after 20 minutes.
Notes: Please read the actual value after 20 minutes’ constant temperature to ensure
the calibration accuracy.
Fig a
Thermometer
Olefin oil
Cone-shaped well

Dry Bath N10 Series Operation manual Chapter 4 Operation Guide
—9 —
If the actual read out of thermometer is 39.6℃,
Modify the temperature to 39.6 by pressing or
. Then press “Start”to confirm the input value.
d) Then the instrument will heat to 100℃automatically, waiting till the instrument is in
100℃thermostat, input temperature calibration value. After 20 minutes’ constant
temperature, the user can read the actual value from thermometer.
Notes: Please read the actual value after 20 minutes’ constant temperature to
ensure the calibration accuracy.
If the actual readout is 101.5 ℃, modify the
temperature in display to 101.5 by pressing or .
Then press “Start” to confirm the input.
Notes: Pressing “Stop”and “”simultaneously during the temperature calibration
indicates exiting the temperature calibration program. The changed value will
be of no effect.
3.2. Calibrated with an external sensor, specific methods are as follows：
Tips ： External sensors are optional parts, it needs to be ordered separately.
a) After the startup of the instrument, it enters into waiting interface. Make sure that the
temperature in display is below 35℃. If the temperature is higher than 35℃, the user
should wait until the temperature is below 35℃.
b) Put one end of the external sensors into a block well, and insert the other end to the
interface. See Fig b.
Fig b

Dry Bath N10 Series Operation manual Chapter 4 Operation Guide
—10 —
c) Press “Stop” key without release, then press 
key at the same time for 3 seconds, it will enter into
temperature calibration interface, see the right.
At this point, “ ” displayed, which indicates entering into external
temperature calibration program, the temperature displayed is external sensor’s
current temperature and begins to rise to 40.0℃automatically.
It will automatically display “ ”six seconds
later, the upper displayed 32.0 is the current
temperature of the external sensor.
When the temperature reaches to 40℃constant
temperature, the decimal digit begins to flicker,
waiting for the calibrated value of 40℃. Read out
the actual value from the thermometer after 20
minutes.
Notes: Please read the actual value after 20 minutes’ constant temperature to ensure
the calibration accuracy.
Press “Stop” key to view the temperature value of
external sensor, if displayed 39.6 °C.
Press “Stop”again, or wait for 6 seconds, then
displayed “ ”, modify the displayed
temperature to 39.6 by pressing or . Then press
to confirm the input value.
d) Then the instruments will heating to 100℃automatically，waiting till the instrument
is in 100℃thermostat, input temperature calibration value. After 20 minutes’
constant temperature, the user can read the actual value from thermometer.
Notes: Please read the actual value after 20 minutes’ constant temperature to ensure
the calibration accuracy.
If the actual readout of sensor is 101.5℃, modify
the temperature in display to 101.5 by pressing 
or . Then press “” to confirm the input value.

Dry Bath N10 Series Operation manual Chapter 4 Operation Guide
—11 —
Notes: Pressing “Stop” and “”simultaneously during the temperature calibration
indicates exiting the temperature calibration Program. The changed value will
be of no effect.
4．Temperature control by external sensor
a) Insert the external sensor into a fixed well.
b) Press “Stop” without release, then simultaneously press “”at Time for 3 seconds,
the external indicator light goes on to indicate that it has entered into the
temperature control mode of external sensor.
c) Press “Run” for program running.
Tips: After entering into the external temperature control mode, the user can
simultaneously press “Stop”and "" on Time to exit, while the external indicator
light goes off.
5．The exchange of metal block
a) Completely screw out the 2 screws which
were used for fixing metal blocks by
combination wrench in an anticlockwise
direction.
b) Fix the wrench in the center well of the block.
c) Pull out the wrench with the block.

Dry Bath N10 Series Operation manual Chapter 4 Operation Guide
—12 —
d) Screw the wrench out of the block, and fix it to another replaced block. Then put
it onto the instrument and fix it well by the wrench.

Dry Bath N10 Series Operation manual Chapter 5 Failure Analysis and Troubleshooting
—13 —
Chapter 5 Failure Analysis and Troubleshooting
Problems and actions
No.
Common problem
Possible cause
Action(s)
1
No display on the
screen
No power on the main
power plug
Check power supply and
plugged properly
Faulty fuse
Change fuse
On/Off button broken
Change button
Others
Contact us
2
The actual and
displayed temperatures
are quite different
Broken sensor or
loose contact of the
block
Contact us
3
“OPEn” displayed,
alarming “beep”
Sensor disconnect
Contact us
“SHOr” displayed,
alarming “beep”
Sensor short-circuit
“HHHH” displayed,
alarming “beep”
Sensor broken, or
block temperature is
too high
4
No heating
Sensor broken
Contact us
Heating tube broken
5
Keys don’t work
Faulty key
Contact us

Dry Bath N10 Series Operation manual Annex
—14 —
Annex：Wiring Diagram
（ for reference only ）
L
PE
NSwitch
USB PT1000
CONN BOARD
J3 J2
J1 AC220V
J4 Heat
POWER
BOARD
J3 HeatCtl
J2 AC9V
J2PT100 J4PT1000USB1 USB
J1 AC9V
X1 HeatCtl
MAIN
BOARD
HEAT
PT100
Block

Memo