NuAire Labgard NU-430-400 Manual

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LabgardESEnergySaverClassII,
TypeB2LaminarFlow
BiologicalSafetyCabinet
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Models
NU‐430‐400/600
Bench/Console
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Operation&MaintenanceManual
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February,2014
(Series65)
Revision1
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 
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ManufacturedBy:
NuAire,Inc.
2100FernbrookLane
Plymouth,MN55447
Toll‐Free:1‐800‐328‐3352
InMinnesota:(763)‐553‐1270
Fax:(763)‐553‐0459

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February/2014
Congratulations!
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YouhavejustpurchasedoneofthefinestLaminarFlowBiologicalSafetyCabinetsavailable.Withpropercare,
maintenance(certification),andlaboratoryprocedure,thiscabinetwillgiveyouyearsofproductandpersonnelprotectionfrom
particulatecontaminantsasprescribedinNSF/ANSI49.Pleasereadthismanualcarefullytofamiliarizeyouwithproperinstallation,
maintenanceandoperationofthecabinet.Otherreferenceandguidelinematerialsareavailablethroughthefollowingwebsites.
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www.hc‐sc.gc.ca
www.cdc.gov/od/ohs/
www.absa.org
www.absa‐canada.org
www.ebsa.be
www.inspection.gc.ca
www.who.int
www.biosafety.be
www.hse.gov.uk
www.nsf.org
www.cetainternational.org
www.osha.gov/dts/osta/
www.nuaire.com

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ABOUTTHISOPERATION&MAINTENANCEMANUAL
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Theinformationcontainedinthismanualisintendedtoreflectourcurrentproductionstandardconfigurationmodelalong
withthemorefrequentlypurchasedoptions.Anyuniqueadditions/modifications/shopdrawingsareappendedinthebackflapof
thismanual,alongwithanymodificationsand/oradditionstoproceduresasoutlinedinthismanual.Acopyoftheoriginalfactory
testreportisalsoappendedtothismanual.Incasethismanualand/ortestreportislostormisplaced,NuAireretainsacopyinour
files.AreplacementcopycanbeobtainedbycallingorwritingNuAire,Inc.statingthemodelnumberandserialnumberandabrief
descriptionoftheinformationdesired.

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LabgardESEnergySaverClassII,TypeB2LaminarFlowBiologicalSafetyCabinet
ModelsNU‐430‐400/600Operation&MaintenanceManual
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TABLEOFCONTENTS
SectionNo.1...........................................................GeneralInformation
SectionNo.2...........................................................Models&Features
SectionNo.3...........................................................Warranty
SectionNo.4...........................................................Shipments
SectionNo.5...........................................................InstallationInstructions
 5.1.................................................................Location
 5.2.................................................................Set‐upInstructions
 5.3.................................................................Exhaust/SupplyAirChecks
 5.4.................................................................CertificationTestingMethodsandEquipment
SectionNo.6...........................................................OperatingtheNU‐430
 6.1.................................................................BiologicalSafetyCabinetControl
 6.2.................................................................OperatingGuidelines
 6.3.................................................................OperatingSequence
 6.4.................................................................Ergonomics
 6.5.................................................................CleaningProcedures
 6.6.................................................................HazardousDrugDecontaminationProcedure
SectionNo.7...........................................................GeneralMaintenance
 7.1.................................................................Decontamination
 7.2.................................................................FluorescentLampBulbReplacement
 7.3.................................................................HEPAFilter/MotorReplacement
 7.4.................................................................SlidingWindowReplacement&Adjustment
 7.5.................................................................AirflowControlSystemSetup&Calibration
 7.6.................................................................HEPAFilterLeakTest
 7.7.................................................................AirflowSmokePatternTest
 7.8.................................................................SiteInstallationAssessmentTests
 7.9.................................................................CleanlinessClassificationTestforPharmacyApplication
 7.10...............................................................MainControlBoardDescription&Replacement
 7.11...............................................................DigitalAirflowSensorDescription&Replacement
SectionNo.8...........................................................ErrorIndicators,Troubleshooting,Option‐Diagnostics&
AirflowSensorPerformanceVerification
SectionNo.9...........................................................RemoteContacts
SectionNo.10.........................................................OptionalEquipment
 10.1.................................................................UltravioletLight
SectionNo.11.........................................................Electrical/EnvironmentalRequirements
SectionNo.12.........................................................DisposalandRecycle
Insert..........................................................................ReplacementPartsList
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MANUALDRAWINGS
ACD‐12153....................................................NU‐430AirflowSchematic
BCD‐15501.....................................................NU‐430‐400SpecificationDrawing
BCD‐15502.....................................................NU‐430‐600SpecificationDrawing
BCD‐11815.....................................................DrainValveInstallation
BCD‐05572.....................................................ButterflyValveInstallation
BCD‐05660.....................................................BagIn/BagOutProcedure
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ASSEMBLYDRAWINGS
BCD‐05147.....................................................BaseStandAssembly
BCD‐05146.....................................................BaseStandStorageCabinetAssembly
BCD‐11817.....................................................ControlCenter&FrontDecorativePanelAssembly
BCD‐11818.....................................................SlidingWindowAssembly&Adjustment
BCD‐14185.....................................................BaseCabinetAssembly
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ELECTRICALSCHEMATICS
BCD‐16606(Sheets1‐2)................................NU‐430‐400/600ElectricalSchematic

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LabgardESEnergySaverClassII,
TypeB2LaminarFlow
BiologicalSafetyCabinet
Models
NU‐430‐400/600
MANUFACTUREDBY:
NuAire,Inc.‐Plymouth,Minnesota,U.S.A.
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1.0 GeneralInformation
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1.1Description
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TheLABGARDESModelNU‐430LaminarFlowBiologicalSafetyCabinet(LFBSC)isabench/tabletopmodel,optionally
availablewithabasesupportstand,foroperationasaconsolemodel.TheLABGARDESmodelNU‐430utilizesanEnergy
SaverDCECMmotoroptimallydeterminedforwardcurvedfanforeachmodelsize/widthtomaximizebothenergy
efficiencyandfilterloadingcapacity.TheEnergySaverECMmotoriscontrolledtoairflowsetpointsviaasolid‐stateDC
motorcontrollerwithdigitaldualthermistorairflowsensorsthatprovideanautomaticcompensation(constantvolume
control)forbothfilterloadingandlinevoltagevariances.
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TheLaminarFlowBiologicalSafetyCabinet,(LFBSC)isaproductresultingfromthedevelopmentofthe"laminarflow"
principleandtheapplicationofenvironmentalcontrolsasrequiredinthefieldofbiologicalresearchorchemical
containment.TheLFBSC,whenusedwithpropertechnique,isaneffectivelaboratoryaidinobtainingtheoptimumcontrol
overproductqualitywhilereducingthepotentialforexposureofbothproductandpersonneltoairbornebiologicalor
particulatechemicalagentsinlowtomoderaterisk‐hazardresearchanddrugpreparationorproductoperations,as
prescribedbytheCenterforDiseaseControl(CDC)Atlanta,Georgia.
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TheNU‐430BenchLFBSCmeetstherequirementsofaClassII,TypeB2sincethecabinetconformstothefollowing
requirements:

-Maintainaminimumaverageinflowvelocityof100fpm(0.51m/s)throughtheworkaccessopening;

-HaveHEPAfiltereddownflowairdrawnfromthelaboratoryortheoutsideair(i.e.downflowairisnotre‐circulated
fromthecabinetexhaustair;
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-Exhaustallinflowanddownflowairtotheatmospherethroughahardconnectiontothefacilityexhaustsystemafter
filtrationthroughaHEPAfilterwithoutrecirculationinthecabinetorreturntothelaboratory;
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-Haveallcontaminatedductsandplenumsundernegativepressureorsurroundedbydirectlyexhausted
(non‐re‐circulatedthroughtheworkarea)negativepressureductsandplenums.
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TypeB2cabinetsmaybeusedforworkwithnonflammableorexplosivevolatilechemicalsandradionuclidesrequired
asadjunctstomicrobiologicalstudies.
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1.2SafetyInstructions
ThesesafetyinstructionsdescribethesafetyfeaturesoftheLABGARDESModelNU‐430LFBSC.
Thesafetycabinethasbeenmanufacturedusingthelatesttechnologicaldevelopmentsandhasbeenthoroughlytested
beforedelivery.However,thecabinetmaypresentpotentialhazardsifitisnotinstalledandusedasinstructedforits
intendedpurposeoroutsideofoperatingparameters.Therefore,thefollowingproceduresmustalwaysbeobserved:
Thesafetycabinetmustbeoperatedonlybytrainedandauthorizedpersonnel.
Foranyoperationofthiscabinet,theoperatormustprepareclearandconcisewritteninstructionsforoperating
andcleaning,utilizingapplicablesafetydatasheets,planthygieneguidelines,andtechnicalregulations,in
particular.
oWhichdecontaminationmeasuresaretobeappliedforthecabinetandaccessories?
oWhichprotectivemeasuresapplywhilespecificagentsareused?
oWhichmeasuresaretobetakeninthecaseofanaccident?
Repairstothedevicemustbecarriedoutonlybytrainedandauthorizedexpertpersonnel.
Keeptheseoperatinginstructionsclosetothecabinetsothatsafetyinstructionsandimportantinformationare
alwaysaccessible.
Shouldyouencounterproblemsthatarenotdetailedadequatelyintheoperatinginstructions,pleasecontactyour
NuAireRepresentativeofNuAiretechnicalServices.
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1.3ExplanationofSymbols
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NOTE:
CAUTION used without the safety alert symbol
indicates a potentially hazardous situation which, if
not avoided, may result in property damage.
Potential electrical hazard, only qualified person
to access.
Used for important information.
Biohazard
Ground, Earth
CAUTION

Lead Free Chemical Hazard
Flammable Hazard
Hazardous Gases! Personal
Protection Equipment
Re
q
uired.
Safety alert symbol indicates a potentially hazardous
situation which, if not avoided, could result in death
of serious injury.
Safety alert symbol indicates a potentially hazardous
situation which, if not avoided, may result in minor or
moderate injury.
WARNING
!
CAUTION
!

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2.0Models&Features
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ThemodelNU‐430,LABGARDESClassII,TypeB2LaminarFlowBiologicalSafetyCabinetismanufacturedintwosizes:
4ft.(1.2m)and6ft.(1.8m).
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3.0Warranty
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NuAire,Inc.warrantsClassII,TypeB2w/ECMMotor(LABGARDES)thatitwillrepairF.O.B.itsfactoryorfurnishwithout
chargeF.O.B.itsfactoryasimilarparttoreplaceanymaterialincludingsupplyHEPAfilter(excludingexhaustHEPAfilter)in
itsequipmentwithin60monthsafterthedateofsaleifprovedtothesatisfactionofthecompanytohavebeendefective
atthetimeitwassoldprovidedthatallpartsclaimeddefectiveshallbereturned,properlyidentifiedtothecompanyatits
factory,chargesprepaid.Factoryinstalledequipmentoraccessoriesarewarrantedonlytotheextentguaranteedbythe
originalmanufacturer,andthiswarrantyshallnotapplytoanyportionoftheequipmentmodifiedbytheuser.Claims
underthiswarrantyshouldbedirectedtoNuAire,Inc.settingforthindetailthenatureofthedefect,thedateoftheinitial
installationandtheserialandmodelnumberoftheequipment.
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ThiswarrantyshallnotapplytoanyNuAireproductorpartthereofwhichhasbeensubjecttomisuse,abuse,accident,
shippingdamage,improperinstallationorservice,ordamagebyfire,floodoractsofGod.Iftheserialnumberofthis
productisaltered,removedordefacedastobeillegible,theWarrantyshallbenullandvoidinitsentirety.
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Thewarrantyisforthesolebenefitoftheoriginalpurchaserandisnotassignableortransferable.Priortoreturningany
item,foranyreason,contactNuAireforaReturnAuthorizationNumber.Thisnumbermustaccompanyallreturns.Any
productshippedtoNuAirewithoutthisnumberwillbereturnedrefusedshipmentorcollectfreight.
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4.0Shipments
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NuAiretakeseveryreasonableprecautiontoassurethatyourLABGARDEScabinetarriveswithoutdamage.Motorcarriers
arecarefullyselectedandshippingcartonshavebeenspeciallydesignedtoinsureyourpurchase.However,damagecan
occurinanyshipmentandthefollowingoutlinesthestepsyoushouldtakeonreceiptofaNuAireLABGARDEScabinetto
besurethatifdamagehasoccurred,theproperclaimsandactionsaretakenimmediately.
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4.1DamagedShipments
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4.1.1Termsarefactory,unlessstatedotherwise.Therefore,itisimportanttocheckeachshipmentbeforeacceptance.
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4.1.2Ifthereisvisibledamage,thematerialcanbeacceptedafterthedrivermakesanotationontheconsignee'scopy
ofthefreightbill.Thenaninspectionmustbemadetoverifytheclaimagainstthecarrier.Thisinspectionisthe
basisofyourfilingtheclaimagainstthecarrier.
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4.1.3IfconcealeddamageisfounditisabsolutelynecessarytoNOTIFYTHEFREIGHTAGENTATONCEandrequestan
inspection.Withoutthisinspection,thetransportationcompanymaynotacceptaclaimforlossordamage.Ifthe
carrierwillnotperformtheinspection,anaffidavitmustbepreparedstatingthathewascontactedonacertain
dateandthathefailedtocomplywiththerequest.Thisalongwithotherpapersinthecustomer'spossessionwill
supporttheclaim.

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5.0InstallationInstructions
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5.1Location
Withinthelaboratory,pharmacy,etc.,theideallocationofthebiologicalsafetycabinetisawayfrompersonneltraffic
lanes,airvents(inorout),doorsand/oranyothersourceofdisruptiveaircurrents.
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Ifdraftsorotherdisruptiveaircurrentsexceedtheinflowvelocityofthecabinetthroughtheaccessopening,thepotential
existsforcontaminatedairtoexitorentertheworkzoneareaofthecabinet.Itdependsontheseverityoftheaircurrent.
REMEMBER:ABIOLOGICALSAFETYCABINETISNOSUBSTITUTEFORGOODLABORATORYTECHNIQUE.
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Wherespacepermits,aclear6inch(152mm)areashouldbepermittedoneachsideofthecabinetformaintenance
purposes.Theelectricaloutletintowhichthecabinetisconnectedshouldbereadilyaccessibleformaintenancepurposes.
Donotpositionthecabinettopreventaccesstothepowercord.Thepowercordplugservesasthedisconnectand
shouldremainreadilyaccessible.Iftheoutletisinaccessible,suchasaconduit(hardwired)connection,thenanappropriate
warninglabelshouldbeappliednearthecabinet'son/offswitch,toindicatethecircuitbreakeronthepowerdistribution
paneltobeused.
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Morethananyotherbiologicalsafetycabinet,theNU‐430requirescarefulsite‐planningandpreparation,duetothetotal
exhaustnatureofthecabinet.Propersizingoftheexhaustandmakeupsupplysystemsarecriticaltothesuccessful
installationofthecabinet.Inaddition,thecabinetprovidesforthechoiceofmake‐upairforthesupply(downflow)air.
Thefollowingareairflowrequirementsbasedonconcurrentbalancevalues*.
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AirVolume(CFM/CMH)SupplyAirInflowExhaustAir**
NU‐430‐400440/748345/586785/1334
NU‐430‐600719/1222531/9021250/2124
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*ConcurrentBalanceValueisdeterminedbyaducttraversemeasurementmethodasspecifiedinASHRAEStandard111at
itsnominalsetpointcalibratedusingtheprimaryDIMmethodandcapturehoodremoved.Thesevaluesshallbeusedfor
designandbalanceexhaust/supplyHVACrequirements.
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**Exhaustairvolumeatnegative1.7inches(43mm)w.g.forNU‐430‐400and
1.8inches(46mm)w.g.forNU‐430‐600.
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5.2Set‐UpInstructions
Removeoutershippingprotection(cartonorcrating).Thecabinetisfastenedtothebaseskidanditisusuallythebest
proceduretoleavetheskidinplaceuntilthecabinetislocatedinitsapproximatepositiontofacilitateeaseinhandling.
Itcanthenberemovedfromtheskidbyremovingthebandingholdingthecabinettotheskid.Itmaybenecessaryto
removetheControlCenterinordertogainpassagethroughadoorway.Itmayeasilyberemovedbyfollowingthe
instructionsondrawingBCD‐11817.
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Itisrecommendedthatnolessthantwopeoplearepresentusinga
liftingsystemforplacementofthecabinetontothebasestand.
Itisnotrecommendedtomanuallyliftthecabinetontothebasestand.
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5.2.1BaseStandAssembly
ThebasestandisshippedknockeddowninaseparatecartonandisassembledperdrawingBCD‐05147if
accompaniedwiththecabinet.Removethebandingholdingthecabinettothebaseskid.Liftthecabinetfrom
thebaseskidandplaceonthefloor.Nowliftthecabinetontopofthebaseandboltthebasestandtothecabinet
usingtwo3/8"‐16x3/4"boltsandwashersprovidedforthefrontbasestandtabsandtwo1/4"acornnutsfor
therearweldstuds.Placethecabinetinitsdesiredlocation.
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Thebasestandstoragecabinetswillusuallybeshippedaccordingtocustomerrequirements.Ifitisshipped
unassembled,itcanbeassembledperdrawingBCD‐05146.Itisrecommendedthattheupperandlowerbase
standbracesbeinstalledfirst,thentherearandbottompanels(theendpanelsarealwaysprefastened).Once
assembled,fastenthecabinetpertheaboveinstructions.
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Removethecapprotectingthedrainvalvethreadsandinstallthedrainvalve,onthebottomrightfrontofthe
cabinetusingLoctite242furnishedtothethreadsandrotatethevalvebodyuntilitissecure(SeeBCD‐11815).
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5.2.2Leveling
Usingalevelplacedontheworktray,adjusttheleglevelers,first,end‐to‐endthenfronttoback.
TheNSFapprovedleglevelersprovidea3/4"(20mm)adjustment.
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5.2.3BenchInstallation(BCD‐11815)
Placethecabinetonthebenchwithapproximatelya2"(51mm)overhangclearanceforinstallationofthedrain
valve.Ifthedrainvalveisnotdesired,capwith3/8”NPTfittingandplacethecabinetinitsdesiredlocationand
usingRTVcaulk,sealallaroundthebaseofthecabinetandthebench.Thisprovidesatightsealtopreventbench
spillsfrommigratingunderthecabinet.
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Ifadrainvalveisdesired,(NOTE,CHECKWITHYOURSAFETYPERSONNELFORREQULATORYREQ
UIREMENTS(i.e.
LOCKINGTYPE)OFDRAINVALVEINSTALLATION)removethehandlefromthevalvestemtogainclearancefor
valvebodyrotation.AddLoctite242(furnished)tothethreadsandrotatevalvebodyuntilsecure,withthevalve
stem(forhandle)ontheleftside.Re‐installhandletovalvestem.Adjustthecabinetonbenchtoprovidea
1‐1/2"(38mm)overhangandsealtheinterfaceofthebenchandcabinet,usingRTVcaulkasabove.
CAUTION
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5.2.4GasService
NuAiredoesn'trecommendtheuseofnaturalgaswithintheLFBSC,butifgasserviceisdeterminedtobe
necessaryfortheapplication,appropriatesafetymeasuresmusttakeplace.AllNuAireLFBSC'shaveprecautionary
warninglabelsthatsaythefollowing:
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   Useofexplosiveorflammablesubstancesinthiscabinet
 shouldbeevaluatedbyyourappropriatesafetypersonnel.
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Oncetheappropriatesafetypersonnelhavemadethedetermination,theapplicationofnaturalgasmust
beperformedinaccordancetonational,stateandlocalcodes.ITISALSOSTRONGLYRECOMMENDEDTHATAN
EMERGENCYGASSHUTOFFVALVEBEPLACEDJUSTOUTSIDETHELFBSCONTHEGASSUPPLYLINE.
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AllNuAireLFBSC'smeetthesafetyrequirementsofULandCSAforLaboratoryEquipment.Tocomplywiththese
safetyrequirements,NuAireusesonlycertifiedgasvalves.Inaddition,ifexternalpipingisrequired,onlyblack
pipeisusedforthisapplication.

AspreviouslystatedNuAiredoesn'trecommendtheuseofnaturalgaswithintheLFBSCandASSUMESNO
RESPONSIBILITYFORITSUSE.USEATYOUROWNRISK.TheBunsenburnerflamewithintheLFBSCnotonly
contributestoheatbuild‐up;isalsodisruptsthelaminarairstream,whichmustbemaintainedformaximum
efficiency.IFTHEPROCEDUREDEMANDSUSEOFAFLAME,ABUNSENBURNERWITHONDEMANDIGNITIONIS
STRONGLYRECOMMENDED.DONOTUSECONSTANTFLAMEGASBURNERS.Duringuse,theBunsenburner
shouldbeplacedtotherearoftheworkspacewhereresultingairturbulencewillhaveaminimaleffect.

5.2.5PlumbingServices
Serviceballvalveswiththetypeofservicespecifiedbytheremovablebuttononthehandlearelocatedinthe
workzone.Theserviceballvalvesarenotrecommendedforpressureover75p.s.i.(5.2BAR).Reducingvalves
shouldbeinstalledexternaltothecabinetifnecessary.Serviceballvalvesshouldneverbeusedforflammable
gassesoroxygenservice.Aspecialneedlevalveforoxygenserviceorcertifiedvalveisrequiredandavailableupon
request.

Externalconnectionisto3/8inchNPTcouplingintheinnersidewalls.Connectiontoplantutilitiesshouldbe
madewithpropermaterialsfortheindividualserviceandaccordingtoNationaland/orLocalcodes.Observeall
labelspertainingtothetypeofserviceandoperatingpressure.

Remotecontrolledneedle‐valveplumbingfixturescanbeoptionallyprovidedwithintheinteriorsidewalls.Control
handlesarelocatedexternallyontheverticalairfoil.Serviceoutletswithintheinteriorhaveserratedtapered
fittingsdesignedforhoseconnectionswiththeremotecontrolledneedlevalveplumbingfixtures.NuAireprovides
forrear,bottom,ortopconnectionsofplumbingservicestoplantutilities.Connectionfromtheneedlevalve
assemblytotheweldedexitcouplingisaccomplishedwiththesupplied3/8inchsoftcoppertubingasstandard
(alternativematerialstomeetlocalcodesareavailableuponrequest).Theneedlevalvesarenotrecommended
forworkingpressureinexcessof125p.s.i.(8.6BAR).

5.2.6ElectricalServices
TheNU‐430seriesBiologicalSafetyCabinetsmaybe"hardwired"(optional)orpluggedintoanoutletwith
protectiveearthingconnectionwiththestandardpowercord.Thecabinetrequires115VAC,60Hzsinglephase
(correctratingvariespercabinetsize,referenceElectrical/EnvironmentalRequirements).Itisrecommendedthat
powertothecabinet,whetherhardwiredorplugconnected,beonitsownbranchcircuit,protectedwithacircuit
breakeratthedistributionpanelnearthecabinet.Asurgeprotectorisstronglyrecommendedifyouare
experiencingpowerrelatedfaults.

PLEASENOTETHISCABINETCONTAINSELECTRONICBALLASTSFORTHEFLUORESCENTLIGHTING.ELECTRONIC
BALLASTSOPERATEWITHHIGHINRUSHCURRENT.ITISNOTRECOMMENDEDTOUSETHISPRODUCTWITH
GROUNDFAULTCIRCUITINTERRUPTERS(GFCI'S)BECAUSETHEBALLASTSMAYCAUSETHEGFCITOTRIP.

CAUTION
!

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5.2.7Exhaust/SupplyDuctInstallationGuidelines
Theexhaust/supplysystemsmustprovideconditionssimilartothatunderwhichthecabinetwascertifiedtomeet
itsstatedperformance.Thefollowingguidelinesshouldbeobservedwheninstallingexhaust/supplyairductwork
foreitherexistingplantexhaustsystems,oranewexhaustsystem.
1.Adequateroommake‐upairinflowtoreplaceexhaustedair.Airdiffusionrateisnottoexceedvelocityof
100LFPM(.51m/s)tominimizedisruptiveaircurrents.Iflaboratoryistobepressurized,follow
guidelinesinANSI/AIHAZ9.5LaboratoryVentilation.

RoomMake‐UpAirRequirements(CFM/CMH)UtilizingConcurrentBalanceValues
ModelWithSupplyDuctWithoutSupplyDuct
NU‐430‐400345/586785/1334
NU‐430‐600531/9021250/2124

2.Adequateplantexhaustsystemcapability.Theexhaustsystemisusuallyadequateifitcanprovidethe
ratedexhaustflowandstaticpressure.
3.Allductlossesmustbeconsideredandaddedtothecabinetlossinselectingtheexhaustblower,fora
newexhaustsystem(i.e.ductdiameter,lengthandnumberofelbows,etc.).
4.Adequatesupplyaircapability(ifused).Thesupplyairsystemisusuallyadequateifitcanprovidethe
ratedsupplyairat0.0incheswatergaugepositive.
5.Thesupplyairmustbeinterlockedwiththeoperationofthecabinet'sinternaldownflowblowerinorder
topreventdownflowairfrombeingforcedoutofthefrontofthecabinetwhenpoweredofforduring
nightsetbackconditions.NuAireprovidesfanrelaycontactsforthispurpose.
6.Allductworkshouldbesecurelyanchoredtothebuildingconstructioninmannertobefreefrom
vibrationandswayingunderallconditionsofoperations.
7.SheetmetalgaugesandseamsshouldbeinaccordancewiththecurrenteditionoftheASHRAEguide.
Aminimumof20gaugeforroundductisrequiredtopreventductcollapseduetohighstaticpressure
conditions(squareductwillrequireheaviergaugematerial).
8.Allductworkshouldbemaintainedatanegativepressurewithinthebuilding(i.e.externallylocated
exhaustblower).
9.Theexhaustblowerandductworkshouldbeasealedsystemthatcanhold2.0inches(51mm)w.g.
pressurefor30minuteswithnomorethana10%dropinpressure,andbeproperlyventedtothe
atmospheretodisperseexhaustedair.
10.Alocalmanualexhaustflowdamper(NuAireModelNU‐940)shouldbereadilyaccessible(eitherdirectly
mountedontheLFBSCexhaustcollarorjustabovetheLFBSC)forthemaintenancetechnician/certifierto
allowtheLFBSCtobesealedfordecontaminationpurposes.IfaConstantAirVolume(CAV)valveis
locatedabovetheLFBSC,anyexhaustflowadjustmentsshouldbemadetotheCAVvalveleavingthe
manualexhaustdamperinafullopenposition.
11.Itisrecommendedthatthecabinetoperationbeinterlockedwiththeexhaustblower.Fanrelaycontacts
areprovidedforthispurpose.However,itisalsorecommendedtohaveamanualexhaustoverride
switchnearthecabinetforcertificationandservice.Formulti‐gangedsystems,thisswitchcouldbeused
tointerfacewithaBuildingAutomatedSystem(BAS).
12.ItisrecommendedthatwhenusingtheNU‐951‐012automaticbutterflyvalve,thesystemairvolume
mustremainwithintenpercentofthegivennominalsetpointvolumetooptimizethemeasurementand
controlperformance.
13.Itisnotrecommendedtohardconnect(i.e.weld)theexhaustconnectiontothecabinet.Thismay
damagetheexhaustfilterand/orthebutterflyvalve(ifpresent).Asiliconsleeve(NuAirePartNo.NU‐
940‐001),bandedbetweenthecabinet'sexhaustductandtheplantexhaustductisrecommended,with
nomorethanatwo‐inchgapbetweentheducts,fora1/8inch(3mm)thicksiliconesleeve.IfNuAire
dampervalvesarepresent,seeDrawingBCD‐05572forinstallation.
14.Ifductdiameterreductionisrequired,itisrecommendedthatthereductionoccursatleast12inches
(305mm)fromthecabinetductconnectionandthatthereductionissmoothandgradualtoreduceair
turbulencethatresultsinnoiseandlossofstaticpressure.
15.ITISNOTRECOMMENDEDTOCONNECTTHECABINETDUCTCONNECTIONDIRECTLYINTOA90‐DEGREE
BEND.Thecabinet'sexhaustairflowsensorcouldbeaffectedbyairflowturbulencecreatedby90‐degree
bends.Ifa90‐degreebendisrequired,itisrecommendedthatthe90‐degreebendoccuratleast12
inches(305mm)fromtheLFBSCexhaustcollar.

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5.2.8FinalAssembly
Removetheprotectivecardboardcoveroverthesupplyandexhaustconnectionsontopofthecabinet.The
exteriorsurfaceandviewingglassareeasilycleanedwithanymildhouseholddetergentcleanerusingasoftcloth.
Harshchemicals,solvent‐typecleanersandabrasivecleanersshouldnotbeused.

DonotattempttocleantheHEPAfiltermedia.Cabinetinteriorwallsorworksurfaceareeasilycleanedwithany
mildhouseholddetergentcleanerusingasoftcloth.Turnthecabinetonandletitoperatefor60minutesbefore
usingitasaLFBSC.

5.3Exhaust/SupplyAirChecks

NOTE:THEINTERNALSUPPLYBLOWERISINTERLOCKEDWITHTHEEXHAUSTSENSOR,
TOPREVENTOPERATIONUNLESSADEQUATEEXHAUSTFLOWISPRESENT.

5.3.1ExhaustVolume/InflowVelocity
Theexhaustvolumeandcorrespondinginflowvelocityisdisplayedonthefrontpanel.Presetlowerandupper
alarmlimitsarefactorysetbutcanbefieldverifiedatanytime.Toinsurethatadequateexhaustisavailablefora
dirtyexhaustHEPAfilter,condition,thenominalexhaustreadingsshouldbeattainablewiththebutterflyvalveor
dampersetat60percentopen,withallotherdampersinthesystem(duct)open.

5.3.2SupplyVelocity
ThesupplyvolumeiscontrolledbytheBSCCairflowcontrolsystem.TheBSCCsystemusesadualthermistor
airflowsensorinthedownflowairstreamtomonitorandcontrolairflowtosetpoint.Thecontrolsystem
automaticallycompensatesforfilterloading,voltagevariancesandotherenvironmentaleffects.However,
excessivevariancescausedbyremotebutterflyvalvesordampers,insufficientsupplyairanddirtyprefiltersmay
causeanairflowalarmconditiontooccur.

NOTE:THESUPPLYAIR,IFINTEGRALLYDESIGNED,MUSTBEINTERLOCKEDWITH
 THEINTERNALFANRELAYTOASSUREPROPEROPERATION.
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5.4CertificationTestingMethodsandEquipment
Afterinstallationandpriortouse,NuAirerecommendsthatthecabinetbecertifiedorcommissionedtofactorystandards.
Asapartofcertification,thecertifiershouldgothroughthefollowinginitialchecklisttoassureallaspectsoftheLFBSC
installationarecompleteandreadyforcertification.

Reviewproductinstallation
Exhaustconnection
Dampervalveinstalledcorrectlywithlabeltowardfront
LFBSCbasestandlevel

Verifyairflowsensorshroudisinplace
Downflow

Verifyconfigurationtypeselectionforspecificmodel*(seesection7.5.2)

Verifysetpointsandalarmlimitsforspecificmodel*(seesection7.5.2)

PerformBCScertification
Ataminimum,thefollowingtestsshouldbeperformed:
HEPAfilterleaktest
Downflowvelocitytest
Inflowvelocitytest
Airflowsmokepatterns
Siteinstallationassessmenttests

PerformSiteAssessmentTests
TheNU‐430requiresverificationofthesupplyfaninterlockandback‐uppressureswitchoperationutilizing
independentexhaustvolumemeasurementinstrument(DIM).PerNSF/ANSI49,a20%lossofexhaust
volumemustproduceanairflowalarmwithin15seconds.

Thetestingmethodsandequipmentrequiredarespecifiedonthefactoryinspectionreportincludedwiththismanual.
(Seeinsertinbackcover)

NOTE: ITISRECOMMENDEDTHATTHESETESTSBEPERFORMEDBYAQUALIFIEDTECHNICIANWHOIS
FAMILIARWITHTHEMETHODSANDPROCEDURESFORCERTIFYINGBIOLOGICALSAFETYCABINETS
(SEEINSERT)

NOTE: AFTERTHEINITIALCERTIFICATION,NUAIRERECOMMENDSTHATTHECABINETBERECERTIFIED(ATA
MINIMUM)ONANANNUALBASISANDAFTEREVERYFILTERCHANGEORMAINTENANCEACTIONOR
ANYTIMETHEOPERATORFEELSITISNECESSARY.

NotethattheLABGARDEScabinets,filters,andsealsprovidepremiumperformance.QualityControlinbothdesignand
manufacturingassuresuperiorreliability.However,protectiontobothproductandoperatorissovitalthatcertificationto
theperformancerequirementsshouldbeaccomplishedasstatedtoensurebiologicalsafetyestablishedbythefactory
standards.

*Ifthespecificmodelisaspecialproductwithnon‐standardsetpointsandalarmlimits,thenewvalueswill
belocatedonthefactoryInspectionReport.

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OM0228Page20of99
February/2014
LabgardESEnergySaverClassII,TypeB2LaminarFlowBiologicalSafetyCabinet
ModelsNU‐430‐400/600

CatalogNumber
CatalogNumberNU‐430‐400
Nominal4foot(1.2m)
NU‐430‐600
Nominal6foot(1.8m)
PerformanceSpecifications
1.PersonalProtection
2.ProductProtection

NSF/ANSI49

NSF/ANSI49
NSFStd.No.49ClassClassII,TypeB2ClassII,TypeB2
StyleofCabinetBenchTop/Consolew/BaseStand/
StorageCabinet
BenchTop/Consolew/BaseStand/
StorageCabinet
CabinetConstructionAllWeldedStainlessSteel16GA,
Type304PressureTightDesign
AllWeldedStainlessSteel16GA,Type
304PressureTightDesign
DiffuserforAirSupply(Metal)Non‐FlammableNon‐Flammable
HEPAFilterSealType:
SupplyFilter‐99.99%Eff.on0.3Microns
ExhaustFilter‐99.99%Eff.on0.3Microns

HEPEXSeal
Neoprene,Springloaded

HEPEXSeal
Neoprene,Springloaded
FumigationperNIH/NSFProcedureYesYes
StandardServices:
ServiceCoupling(3/8inchNPT)
GasValve/ServiceCoupling(3/8inchNPT)
DuplexOutlet

None
Two,RightSidewall
One,LeftFrontFaring

None
Two,RightSidewall
One,LeftFrontFaring
OptionalServices:GasCocks3/8"NPT
**RemoteControlledValves
UltravioletLight
Standard/CupSinks
Upto3ea.Sidewall
Upto3ea.Sidewall
One,Backwall
LeftorRightWorkSurface
Upto3ea.Sidewall
Upto3ea.Sidewall
One,Backwall
LeftorRightWorkSurface
CabinetSizeInches(mm):
Height(FullyAssembled)
Height(MinimumforTransport)
Width
Depth(withControlCenter)

61(1549)
61(1549)
535/8(1362)
327/8(835)

61(1549)
61(1549)
775/8(1972)
327/8(835)
WorkAccessOpeningInches(mm):
StandardOpeningHeight
StandardInflowVelocity

8(203)
105FPM(.53m/s)

8(203)
105FPM(.53m/s)
WorkZoneInches(mm):Height
Width
Depth
251/2(648)
463/8(1178)

251/2(648)
703/8(1788)
231/2(597)
ViewingWindowInches(mm):
StandardisTemperedSlidingGlass
HingedTemperedGlass(optional)
1.0(25mm)Closed
181/2(470)Open
8(203)AccessOpening
1.0(25mm)Closed
181/2(470)Open
8(203)AccessOpening
CertificationExhaustValueCFM/CMH
ConcurrentBalanceValueCFM/CMH+
754/1281
785/1334
1100/1867
1250/2124
PlantDuctStaticPressureEng./Metric1.7”w.g./43mmw.g.1.8”w.g./46mmw.g.
HeatRejected,BTU,PerHour474584
Electrical:115V
Volts,AC(Hz)
++Amps:Blower/Lights
Amps:Outlet
RatedAmps:
12ft.PowerCord(one)
U.L./UL‐C,USListed
115,60
2.1
3
10
14GA‐3Wire,15A
U.L./UL‐C,USListed
115,60
2.6
3
10
14GA‐3Wire,15A
CratedShippingWeight:
NetWeight
570lbs./259kg.
520lbs./236kg.
760lbs./345kg.
710lbs./322kg.
**Remotecontrolledvalvehandlesprojectthroughfrontfairing.Decorativesidepanelsareavailabletocoverplumbing.
+ConcurrentBalanceValueshallbeusedfordesignandbalanceexhaust/supplyHVACrequirements.
++Basedoncabinetwithnewfiltersrunningat115VAC.