Olimpus CKX41 User manual

INSTRUCTIONS
CKX41/CKX31
CULTURE MICROSCOPES
This instruction manual is for the Olympus Culture Microscopes Models CKX41 and CKX31. To
ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this
microscope, we recommend that you study this manual thoroughly before operating the microscope.
Retain this instruction manual in an easily accessible place near the work desk for future reference.
A X 7 3 5 1
Ryf AG
Bettlachstrasse 2
2540 Grenchen
tel [phone]
fax [phone]
www.ryfag.ch

CKX41/CKX31
CONTENTS
IMPORTANT — Be sure to read this section for safe use of the equipment. — 1-3
1 NOMENCLATURE
2 CONTROLS
3 SUMMARY OF OBSERVATION PROCEDURE
4 USING THE CONTROLS
4-1 Microscope Frame ............................................................................................................................................................................... 9
1Turning On the Light Source 2Adjusting the Brightness
3Adjusting the Tension of the Coarse Adjustment Knob
4-2 Stage..................................................................................................................................................................................................................... 10
1Placing the Specimen 2Moving the Specimen
4-3 Observation Tube ....................................................................................................................................................................... 11- 14
PROPER SELECTION OF THE POWER SUPPLY CORD........................................................ 30-31
4-5
6-7
8
9-15
1Adjusting the Interpupillary Distance 2Adjusting the Diopter
3Using the Eye Shades 4Using Eyepiece Micrometer Disks
5
Selecting the Light Path (U-TR30-2 Only) 6Adjusting the Tilt
4-4 Illumination Column........................................................................................................................................................................ 15
1Using the Filters 2Using the Aperture Iris Diaphragm
3Removing the Condenser Lens
4-5 Objective Correction Collars ............................................................................................................................................. 16
1Correction Collar
5 PHASE CONTRAST OBSERVATION
6 PHOTOMICROGRAPHY & TV OBSERVATION
7 TROUBLESHOOTING GUIDE
8 SPECIFICATIONS
9 ASSEMBLY
— See this section for the replacement of the light bulb. —
17- 19
20
21-22
23-24
25-29
LAMP SOCKET INSPECTION SHEET
10 32

1
CKX41/CKX31
Observation tube Binocular tube fixed Replaceable*
Stage center plate – Replaceable**
Fig. 1
Fig. 2
IMPORTANT
The difference between the CKX31 and CKX41 microscopes lie in the following basis systems.
CKX31 CKX41
Reflected fluorescence system Not mountable Mountable
*The U-CBI30-2/U-BI30-2/U-CTBI/CKX-TBI binocular tube or U-CTR30-2/U-TR30-2 trinocular tube can be
mounted. But no intermediate attachment can be mounted.
**The standard stage center plate can be replaced with the CK40-CPG glass center plate, IX-CP50 center
plate (50 mm), etc.
SAFETY PRECAUTIONS
1. After the equipment has been used in an observation of a specimen
that is accompanied with a potential of infection, clean the parts
coming in contact with the specimen to prevent infection.
· Moving this product is accompanied with the risk of dropping the speci-
men. Be sure to remove the specimen before moving this product.
· In case the specimen is damaged by erroneous operation, promptly
take the infection prevention measures.
2. Install the microscope on a sturdy, level table or bench (Weight: CKX31
about 8 kg, CKX41 about 8.8 kg).
3. When transporting the microscope, be sure to keep it horizontal by holding
it by the root of the observation tube relay tube 1and the illumination
column 2. (Fig. 1)
Be sure to remove the specimen since it may fall.
# Do not tilt it during carrying to prevent damaging the desktop or
microscope.
4. Culture liquid or water spilt on the stage, objective or microscope frame
may damage the equipment. Immediately disconnect the power cord
from the wall outlet and wipe the liquid or water off if it is spilt on them.
5. The surfaces of the lamp socket 3of the illumination column will become
extremely hot during operation. When installing the microscope, make
sure to allow ample free space around and in particular above the lamp
socket. (Fig. 1)
When using the TV camera or photomicrographic system, install them so
that the connection cable does not come in contact with the lamp socket.
6. To avoid potential shock hazards and burns when replacing the light
bulb, set the main switch 4to “ ” (OFF) then disconnect the power cord
from the wall outlet in advance. Whenever you replace the bulb during
use or right after use, allow the lamp socket 3and bulb to cool before
touching. (Figs. 1 & 2)
Designated
bulbs:
6V30WHAL high-intensity halogen bulb
(PHILIPS 5761)
7. Always use the power cord provided by Olympus. If no power cord is
provided, please select the proper power cord by referring to the section
“PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of
this instruction manual. If the proper power cord is not used, product
safety performance cannot be warranted.
8. Always ensure that the grounding terminal of the microscope and that
of the wall outlet are properly connected. If the equipment is not grounded,
Olympus can no longer warrant the electrical safety performance of the
equipment.
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2
Safety Symbols
The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment
in the safest possible manner.
Symbol Explanation
Indicates that the surface becomes hot, and should not be touched with bare hands.
Indicates that the main switch is ON.
Indicates that the main switch is OFF.
Before use, carefully read the instruction manual. Improper use could result in personal injury to
the user and/or damage to the equipment.
Warnings
Warning engraving is placed at parts where special precaution is required when handling and using the microscope.
Always heed the warnings.
Warning engraving
position
Lamp socket (U-LS30-3)
[Warning against high temperature]
1Getting Ready
1. A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact.
2. Do not use the microscope where it is subjected to direct sunlight, high temperature and humidity, dust or vibrations. (For
the operating conditions, see chapter 8, “SPECIFICATIONS”.)
3. Use the tension adjustment ring to adjust the rotation tension of the coarse adjustment knob.
4. For the applicable objectives, see chapter 8, “SPECIFICATIONS”. Olympus cannot guarantee the performance when other
objective than specified is used.
9. The microscope system is unstable when the large camera back
is attached. When pulling out the film, be sure to hold the micro-
scope with one hand.
10. Always turn the light intensity control knob 5gently. Do not at-
tempt to turn it beyond the stop position. (Fig. 2)
11. The standard service life of the lamp socket is 8 (eight) years of
use or 20,000 hours of total power ON period, whichever is the
shorter period.
For details, see Inspection Sheet on page 32.

3
CKX41/CKX31
2Maintenance and Storage
1. To clean the lenses and other glass components, simply blow dirty away using a commercially available blower and wipe
gently using a piece of cleaning paper (or clean gauze).
If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute
alcohol.
Since the absolute alcohol is highly flammable, it must be handled carefully.
Be sure to keep it away from open flames or potential sources of electrical sparks –– for example, electrical
equipment that is being switched on or off.
Also remember to always use it only in a well-ventilated room.
2. Do not attempt to use organic solvents to clean the microscope components other than the glass components. To clean
them, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent.
3. Be careful not to spill any liquid –– such as a culture solution –– on the unit. If you do spill anything, immediately set the
main switch to “ ” (OFF) and unplug the power cord. Then wipe away any liquid on or under the objectives.
4. If no objectives are mounted, be sure to cover the objective mounting threaded positions on the revolving nosepiece to
prevent and dust and spilled culture solution from getting on the lenses inside.
5. Do not disassemble any part of the microscope as this could result in malfunction or reduced performance.
6. When not using the microscope, keep it covered with a dust cover. Make sure the lamp socket is cool before covering the
microscope.
7. Using a device that radiates ultraviolet light such as a germicidal lamp near the microscope may discolor (yellow) parts
of the microscope surface. The amount of discoloration depends on the radiation intensity of the ultraviolet light and the
distance between the microscope and radiation source. When not using the microscope, cover it with the dust cover. We
recommend that you also cover the microscope with an impermeable sheet.
8 When disposing of the microscope, check the regulations and rules of your local government and be sure to observe
them.
3Caution
If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition,
the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.
The following symbols are used to set off text in this instruction manual.
: Indicates that failure to follow the instructions in the warning could result in bodily harm to the
user and/or damage to equipment (including objects in the vicinity of the equipment).
# : Indicates that failure to follow the instructions could result in damage to equipment.
} : Indicates commentary (for ease of operation and maintenance).
NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection
against harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in
accordance with the instruction manual, may cause harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause harmful interference in which case
the user will be required to correct the interference at his own expense.
FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance
could void the user’s authority to operate the equipment.
This device complies with the requirements of directive 98/79/EC concerning in vitro diag-
nostic medical devices. CE marking means the conformity to the directive.
4Intended use
This instrument has been designed to be used to observe magnified images of specimens in routine and research
applications.
Do not use this instrument for any purpose other than its intended use.

4
NOMENCLATURE
CKX31
Objectives
PlanCN 4X
PlanCN 10X
PlanCN 20X
PlanCN 40X
PlanCN 60X
PlanCN 100XO
Eyepieces (10X)
Binocular Tube (Fixed)
Microscope Frame
· CKX31SF
Lamp Socket
U-LS30-3
Illumination
Column (Fixed)
Phase Contrast Slider
· Pre-centered Ph slider: IX2-SLP
· Ph centering slider: IX2-SL
Ultralong Working
Distance Condenser
Revolving Nosepiece (Fixed)
Quadruple revolving nosepiece
Stage (Fixed)
· Stage extension plate: CK2-SS
· Mechanical stage: CK40-MVR
For brightfield observation:
#Dedicated objectives are required
for phase contrast observations
(see page 17).

5
CKX41/CKX31
CKX41
Objectives
For brightfield observation:
PlanCN 4X
PlanCN 10X
PlanCN 20X
PlanCN 40X
PlanCN 60X
PlanCN 100XO
#Dedicated objectives are required for phase
contrast observations (see page 17).
Eyepieces
· WHB10X*
· WHN10X/WHN10X-H**
Observation Tube
· Binocular tube: U-CBI30-2*/U-BI30-2**
· Trinocular tube: U-CTR30-2*/U-TR30-2**
· Tilting binocular tube: U-CTBI***/CKX-TBI*
*/** Only the eyepieces carrying the same
marking “ *" or “ ** " as the observa-
tion tube marking can be attached.
*** Dedicated 10X eyepiece built in.
Microscope Frame
CKX41SF
Lamp Socket
U-LS30-3
Illumination Column
(Fixed)
Phase Contrast Slider
· Pre-centered Ph slider: IX2-SLP
· Ph centering slider: IX2-SL
Ultralong Working
Distance Condenser
Stage Center Plate
· Standard stage center
plate
· Glass stage center plate:
CK-40CPG
· Stage center plate
(50 mm): IX-CP50
Revolving Nosepiece (Fixed)
Quadruple revolving nosepiece
Stage (Fixed)
· Stage extension plate: CK2-SS
· Mechanical stage: CK40-MVR

6
CONTROLS
CKX31
}If you have not yet assembled the microscope, read chapter 9, “ASSEMBLY” (pages 25 to 29).
Filter holder (Page 29)
Aperture iris diaphragm
lever (Page 15)
Interpupillary distance
scale (Page 11)
Diopter adjustment ring
(Page 12)
Light intensity control knob
(Page 9)
Phase contrast slider
(Page 16)
Fine adjustment knob
Coarse adjustment knob
Coarse adjustment knob tension
adjustment ring (Page 9)
Main switch (Page 9)
Slider centering knob
storage holes

7
CKX41/CKX31
CKX41
}If you have not yet assembled the microscope, read chapter 9, “ASSEMBLY” (pages 25 to 29).
Filter holder (Page 29)
Aperture iris diaphragm
lever (Page 15)
Interpupillary distance
scale (Page 11)
Diopter adjustment ring
(Page 12)
Light intensity control knob
(Page 9)
Phase contrast slider
(Page 16)
Slider centering knob
storage holes
Fine adjustment knob
Coarse adjustment knob
Coarse adjustment knob tension
adjustment ring (Page 9)
Main switch (Page 9)
Standard stage center
plate

8
10.
SUMMARY OF OBSERVATION PROCEDURE
1. Set the main switch 1to “ I” (ON) and turn the light intensity control knob
2to obtain appropriate brightness. (Page 9)
2. When using the U-TR30-2 trinocular tube, push in the light path selector
knob 3to set the light path at 100% for binocular observation. (Page 14)
3. Place a specimen on the stage. (Page 10)
4. Turn the revolving nosepiece to bring the 10X objective into the light path.
Be sure to turn the revolving nosepiece until it clicks.
5. Adjust the interpupillary distance of the eyepieces. (Page 11)
6. Adjust the diopter of the eyepieces. (Page 12)
7. Bring the required objective into the light path and focus on the specimen.
8. When using the 40X objective provided with the correction collar, set the
scale on the correction collar according to the thickness of the vessel
bottom. (Page 16)
}When performing phase contrast observation, see pages 17 and after.
9. When observing an undyed specimen with brightfield, stop down the
aperture iris diaphragm 4. In phase contrast observation, set the aperture
wide open. (Page 15)
Bring the required filter into the light path. (Page 15)
In brightfield observation, use the LBD filter. In phase contrast observation,
use the IF550 green filter as required.
}In photomicrography, it is recommended to use the 45HA heat absorbing
filter.
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CKX41/CKX31
Fig. 3
Fig. 4
Fig. 5
USING THE CONTROLS
4-1 Microscope Frame
1Turning On the Light Source (Fig. 3)
Set the main switch 1on the side panel of the microscope frame to “ I”
(ON).
2Adjusting the Brightness (Fig. 4)
Turn the light intensity control 1clockwise to raise the voltage and increase
the brightness. Turn it counterclockwise to lower the voltage and decrease
the brightness.
}The service life of the bulb can be extended by using the bulb at a lower
voltage.
3
# Be sure to use the tension adjustment ring 1to adjust the rotation
tension of the coarse adjustment knob.
Adjustment Procedure
Turn the tension adjustment ring 1with your fingers or using a flat-blade
screwdriver. When the ring is turned in the direction of the arrow, tension
of the coarse adjustment knob increases. Turning the ring in the opposite
direction decreases the tension.
If the revolving nosepiece descends on its own or if the specimen gets
out of focus quickly even when it is brought into focus using the fine
adjustment knob, it means that the tension of the coarse adjustment
knob is too low. Turn the ring in the direction of the arrow to increase the
tension.
Adjusting the Tension of the Coarse
Adjustment Knob (Fig. 5)
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Fig. 6
Fig. 7
4-2 Stage
1Placing the Specimen (Figs. 6 & 7)
Put the specimen in the center of the stage.
When Using a 35 mm Petri Dish
}With the CKX41, a 35 mm petri dish can be mounted directly on the
stage provided that the standard stage center plate is in use.
1. With the CKX31, put the provided 35 mm petri dish holder 1on the stage
and mount the 35 mm petri dish on the opening in the center.
2. To move the petri dish, slide the entire holder.
Using the Mechanical Stage
1. When using a 96-well or 24-well micro-titer plate, extend the specimen
holder 2to directly hold the micro-titer plate. (Fig. 7)
2. To hold any other type of plate, combine one of the following provided
holders with the mechanical stage.
· Terasaki holder 3(AB4488): For Terasaki plate, 35 mm petri dish holder
4or 65 mm petri dish.
· Slide glass holder 5(AB4489): For slide glass, 54 mm petri dish.
· Blood cell test plate holder IX2-BCTP 6 (optional):For a blood cell test
plate holder or other calculating chamber for bacteria and eosinophil
with mounting section dimensions corresponding to H 77 x V 35
x D 2 mm, or for a 60mm petri dish.
3. The specimen can be moved to the desired position by turning the X-axis
knob 7and Y-axis knob 8(Stroke: 120 mm in X-axis direction, 78 mm in
Y-axis direction).
2Moving the Specimen
Turn the X-axis and Y-axis knobs of the mechanical stage or move the
specimen directly by hand.
#Be careful when changing objectives. When objectives are switched
after observing the specimen with an objective with short working
distance, the newly selected objective may interfere with the stage
center plate or petri dish holder.
}With the CKX41, the IX-CP50 stage center plate ( 50 mm) allows a wide
range of use without interference.
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11
CKX41/CKX31
Fig. 8
Fig. 9
Fig. 10
4-3 Observation Tube
1 Adjusting the Interpupillary Distance (Figs. 8 to 10)
With the CKX31
While looking through the eyepieces, move both eyepieces until the left
and right fields of view coincide completely.
Adjust so that the two index dots · 1are horizontal. (Fig. 9)
}To make the line connecting the two index dots horizontal, adjust so that
the index dots come on the extension of one of the horizontality lines
inscribed on the pivot.
When your interpupillary distance is other than 50, 60, 70 and 75, adjust
so that the line connecting the two index dots are in parallel with the
horizontality lines on the pivot. (Fig. 9)
}Note your interpupillary distance so that it can be quickly duplicated.
With the CKX41
}When the observation tube is the U-CBI30-2, U-CTR30-2 or U-CTBI, fol-
low the procedure in "With the CKX31".
}When the observation tube is the U-BI30-2, U-TR30-2 or CKX-TBI only
one index dot is provided. (Fig. 10)
· While looking through the eyepieces, move both eyepieces until the left
and right fields of view coincide completely. The position of index dot ·
indicates the interpupillary distance.
}Note your interpupillary distance so that it can be quickly duplicated.
@
When adjusting the interpupillary distance, take care not to let your
fingers get caught in the gaps of the binocular section.

12
Fig. 11
Fig. 12
Fig. 13
Fig. 14
2Adjusting the Diopter (Figs. 11 to 13)
With the CKX31
1. While looking through the left eyepiece with your left eye, turn the coarse
and fine focus adjustment knobs to bring the specimen into focus.
2. While looking through the right eyepiece with your right eye, turn only the
diopter adjustment ring 1to focus on the specimen. (Fig. 11)
With the CKX41
}When the U-CTBI is used, align the white dot · with the index line on the
scale of the right eyepiece’s diopter adjustment ring.
1. While looking through the right eyepiece with your right eye, turn the coarse
and fine focus adjustment knobs to bring the specimen into focus.
2. While looking through the left eyepiece with your left eye, turn only the
diopter adjustment ring 2to focus on the specimen. (Fig. 12)
Using a Finder Eyepiece
}Insert the finder eyepiece into the right eyepiece sleeve of the U-TR30-2
trinocular tube.
1. Looking through the right eyepiece with your right eye, turn the eyepiece
top ring until clearly defined double crosslines can be seen in the field of
view. (Fig. 13)
2. Looking through the right eyepiece, turn the coarse and fine adjustment
knobs to bring the specimen and double crosslines into simultaneous
focus.
3. Looking through the left eyepiece with your left eye, turn the diopter ad-
justment ring to focus on the specimen.
3Using the Eye Shades (Fig. 14)
When Wearing Eyeglasses
Use with the eyeshades in the normal, folded-down position. This will
prevent the eyeglasses from being scratched.
When Not Wearing Eyeglasses
Extend the folded eyeshades in the direction of the arrow to prevent
extraneous light from entering between the eyepieces and eyes.
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CKX41/CKX31
Fig. 15-1
4Using the Eyepiece Micrometer Disk (Figs. 15)
}When the WHN10X-H (or WHN10X) eyepieces are used, an eyepiece
micrometer disk can be inserted in one of them. When the eyepiece
does not have a diopter adjustment mechanism, however, it is hard to
focus on the micrometer disk if the operator has poor eyesight. Should
that be the case, adjust the focus with eyeglasses on.
Use an eyepiece micrometer disk with a diameter of 24 mm and thick-
ness of 1.5 mm.
Following Fig. 15-1, turn the built-in micrometer-mounting frame ² coun-
terclockwise to remove it from the eyepiece and place a micrometer disk
@ into the mounting frame. The engraving on the eyepiece micrometer
disk should face downward in the micrometer-mounting frame.
Screw the micrometer-mounting frame back into the eyepiece.
}When the WHB10X-H (or WHB10X) eyepieces are used, an eyepiece
micrometer disk with a diameter of 20.4 mm and thickness of 1 mm can
be inserted in one of them using the 20.4RH reticle holders ³ (2-piece
set). When the eyepiece does not have a diopter adjustment mecha-
nism, however, it is hard to focus on the micrometer disk if the operator
has poor eyesight. Should that be the case, adjust the focus with eye-
glasses on.
When the reticle holders are used, the field number becomes 19.6.
The field number of the U-CTBI's built-in eyepieces is 18, so it does not
change.
1. Remove both eyepieces. (When the U-CTBI is used, remove only the
right eyepiece by unscrewing the clamping screw on it with a precision
flat-blade screwdriver.)
2. Place an eyepiece micrometer disk | in one of the reticle holders ³ so
that the engraving on the eyepiece micrometer disk faces downward.
3. Screw the reticle holder ³ containing the eyepiece micrometer disk |
into the bottom of the eyepiece.
At the end of screwing, turn the reticle holder by hooking your nail on its
notch 5to screw it all the way in.
4. To provide the other eyepiece with the same field number, screw in the
other reticle holder, without eyepiece micrometer disk, into the bottom of
the other eyepiece.
5. Replace the eyepiece(s) in its(their) original position(s).
WHN10X-H
1
2
Fig. 15-2
3
4
5
WHB10X-H

14
Fig. 16
5Selecting the Light Path (U-TR30-2 Only) (Fig. 16)
Slide the light path selector knob 1to select the desired light path.
Light Path
Selector Knob Symbol Intensity Ratio Applications
Pushed in 100% for binocular
eyepieces
Observation of dark
specimens
Middle position 20% for binocular
eyepieces, 80% for
TV/photography
Observation of
bright specimens,
photography, TV
observation
Pulled out 100% for TV/
photography
Photography, TV
observation
#The U-CTR30-2 trinocular observation tube does not have the light
path selector knob and its light intensity ratio is fixed at 50% binocular
and 50% TV/photography.
Fig. 17
6Adjusting the Tilt (Fig. 17)
}When using the U-CTBI or CKX-TBI, adjust the height and tilt of the obser-
vation tube to obtain the most comfortable viewing position.
Holding the binocular section with both hands, raise or lower it to the
desired position.
· U-CTBI/CKX-TBI: 30 to 60°
# Never attempt to force the binocular section past the upper or lower
stop position. Applying excessive force could destroy the limiting
mechanism.
# The connectable eyepieces are the WHB10X only or the CKX-TBI
and the built-in eyepieces (10X) for the U-CTBI. Combination with
any other eyepiece will result in insufficient illumination at the pe-
riphery of the field of view.
When adjusting the tilt of the U-CTBI, be careful not to have your
fingers caught by the spaces @ between the binocular assembly
and cover.
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CKX41/CKX31
Aperture iris
diaphragm image
Fig. 18
Fig. 19
4-4 Illumination Column
1Using the Filters
}Using appropriate filters according to the purposes allows you to observe
and photograph specimens more effectively. Particularly, the use of the LBD
filter is recommended in observation and photomicrography because it
renders more neutral colors.
}More than one filter can be stacked in the filter holder (Filter diameter: 45
mm. Maximum thickness of stacked filters: 11 mm).
Filter Application
45IF550-W45 Monochrome contrast filter (Green)
45ND6, 45ND25 Light intensity adjustment filter
(Transmittance 6% and 25%)
45LBD2-N, 45KB Color temperature conversion filter
(For observation and photomicrography)
45HA
(Heat absorbing filter)
Exposure time compensation in photomicrogra-
phy
2Using the Aperture Iris Diaphragm (Fig. 18)
}The aperture iris diaphragm determines the numerical aperture of the
illumination system in brightfield observation. It enables you to adjust the
depth of focus, contrast and resolution according to your requirements.
· Checking the aperture iris diaphragm:
Remove the eyepiece when necessary (and insert the CT-5 or U-
CT30 if you have one). Then look into the eyepiece sleeve; you
will seen the field of view as shown in Fig. 18. Now adjust the
aperture iris diaphragm lever as required.
· In general, when observing a dyed specimen, set the aperture iris dia-
phragm to 70% to 80% of the N.A. of the objective in use. However, when
observing a culture specimen, which is not dyed, set the aperture iris
diaphragm lever toward “ ”.
3Removing the Condenser Lens (Fig. 19)
}To provide more working distance, turn the condenser’s lower section 1
in the direction of the arrow and remove it. When you do this, however,
keep in mind that proper illumination cannot be achieved. Remove the
condenser lens only when using a large culture vessel.
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Objective pupil
70-80%
30-20%

16
Fig. 20
4-5 Objective Correction Collars
1Correction Collar (Fig. 20)
}A culture microscope is designed to observe specimens contained in
vessels of various bottom thickness values. In order to achieve optimum
objective performance of the culture microscope, the LUCPlanFLN20X,
40X, 60X, etc. are provided with a correction collar @.
The correction is possible according to the vessel thickness.
1. If the thickness of the vessel bottom is known: Match the correction collar
to the thickness of the vessel bottom using the collar scale ² provided.
2. If the thickness of the vessel bottom is unknown:
The optimum position for the correction collar can be obtained from the
image resolution. If a satisfactory sharp image is not obtained after focus
adjustment, rotate the correction collar to the left and right so that you
can compare the images at both sides. Reset the collar to the better
image, then starting from this position, further rotate the collar to the left and
right until both images can be obtained for comparison. By repeating this
procedure several times, you will find best position for the correction collar.
Refocus after rotating the correction collar.
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