Nikon SMZ645 User manual

Stereoscopic Zoom Microscope
SMZ645/ SMZ660
Instructions
M219 E 06.2.NF.4

1
Thank you for purchasing the Nikon product.
This instruction manual is written for the users of Nikon
Stereoscopic Zoom Microscopes.
To ensure correct usage, read this manual carefully before
operating the instrument.
•It is prohibited to reproduce or transmit this manual in part or
whole without Nikon’s expressed permission.
•The contents of this manual are subject to change without notice.
•Although every effort has been made to ensure the accuracy of
this manual, if you note any points that are unclear or incorrect,
contact your nearest Nikon representative.
•Some of the products described in this manual may not be
included in the set you have purchased.
•If the equipment is used in a manner not specified by the
manufacturer, the protection provided by the equipment may be
impaired.
WARNING
CAUTION
Warning/Caution Symbols Used in This Manual
Though Nikon products are designed to provide you with the utmost
safety during use, incorrect usage or disregard of the instructions
may cause personal injury or property damage. For your own
safety, read the instruction manual carefully and thoroughly before
using the product. Do not discard this manual. Always keep it near
the product for easy reference. Inside this instruction manual, safety
instructions are indicated with the symbols shown below.
Be sure to follow the instructions marked with these symbols for
your safety.
Symbol Meaning
Disregarding instructions marked with this
symbol may lead to death or serious injury.
Disregarding instructions marked with this
symbol may lead to injury or property
damage.

2
WARNING
1. Intended use of this product
This product is intended only for microscopy. Do not
use it for any other purpose.
2. Do not disassemble
Disassembly may result in damage to the instrument.
Never disassemble any part except as described in this
operation manual. Contact your Nikon representative if
you notice any malfunction of this instrument.
3. Check the input voltage
When using an illuminator, check that the input voltage
displayed on the power supply of the illuminator
matches the operating voltage. Contact your Nikon
representative if the displayed voltage does not match the
operating voltage. Use of an improperly matched
illuminator may result in damage to equipment.
4. Power cord
Always turn the power switch off and unplug the power
cord when replacing the lamp of an illuminator as failure
to do so may result in electric shock or equipment
damage.
5. Heat from the light source
When using an illuminator, do not place cloth or paper or
highly flammable materials, such as gasoline, benzene,
thinner or alcohol, near the lamp as there is a danger of
fire.
•Always use lamps that are of the specified rating only.
For the lamp rating, please refer to the operation
manual.
•Always turn the power switch off and unplug the
power cord when replacing the lamp of a illuminator
as failure to do so may result in electric shock or
equipment damage.
Be sure to always follow these guidelines.

3
CAUTION
1. Caution when replacing lamps
The lamp and the surrounding area become very hot
during and immediately after using the illuminator. Be
careful not to burn yourself. In addition, when replacing
a lamp be sure the lamp has cooled sufficiently.
2. Check the light source
Always use lamps that are of the specified rating only.
Use of a lamp having a different specification may result
in damage to equipment. For the lamp rating, please
refer to the operation manual.

4
1. Installation location
Note the following points when installing the stereoscopic
microscope.
•Install the microscope in a location with the temperature between
0° and 40°C, and humidity of less than 80%. If installed in a hot
and humid location, mold may form on the lenses or condensation
may occur inside, resulting in reduced performance or damage to
the microscope.
•Do not install the microscope in a location subject to direct
sunlight.
•Install the microscope in a location that is not subject to vibration.
•Install the microscope in a location that is free from dust and dirt.
2. Handle the microscope carefully
The stereoscopic microscope is a precision optical instrument.
Handle it carefully and do not subject it to impact. Shock from
impact during transportation or operation as well as forcible
operations may cause damage to the instrument.
3. Cleaning the lenses
Do not let dust, fingerprints, etc. to get on the lenses. Dirt on the
lenses will adversely affect the view of the image. If any lenses
become dirty, clean them as described below.
•Use an air blower to blow dust away. If this does not suffice, brush
away the dust with a soft brush or gently wipe it away with a piece
of gauze.
•Only if the lenses become dirty with fingerprints or grease stains,
slightly dampen a piece of soft, clean cotton cloth with absolute
alcohol (ethyl alcohol or methyl alcohol) and gently wipe away the
dirt. Do not use the same part of the cloth more than once.
•Since absolute alcohol is highly flammable, be careful in handling
it so that it does not ignite.
•Observe the manufacturer’s handling instructions when handling
absolute alcohol.

5
Be sure to always follow these guidelines.
4. Cleaning painted or plastic parts
Use of silicon cloth is recommended when cleaning painted parts,
plastic parts and printed parts. If such a part becomes excessively
dirty, wipe it gently with gauze dampened in a mild detergent
solution. Do not use organic solvents (such as alcohol, ether, or paint
thinner) as this may result in deformation of the part or remove of
lettering.
5. Storage
Store the microscope in a location with low humidity where mold is
unlikely to form. Do not store the microscope in a location subject
to direct sunlight or high temperature and humidity. During storage,
place a plastic cover over the equipment to prevent dust
accumulation.
6. Regular inspections
Regular inspections are recommended in order to maintain peak
performance. Please consult your Nikon representative for details
about regular inspections.

7
Contents
Warning/Caution Symbols Used in This Manual ...........................1
Be sure to always follow these guidelines. ....................................2
WARNING ............................................................................................2
CAUTION .............................................................................................3
INomenclature ...................................................................................8
II Assembly .........................................................................................10
III Usage................................................................................................ 12
1Preparations For Observation ......................................................12
1. Adjust the torque of the focus knob.......................................12
2. Adjust the interpupillary distance. .........................................12
3. Adjust the diopter. .................................................................12
2Focusing ......................................................................................13
1. Check the working distance...................................................13
2. Focus on the sample. .............................................................13
3Zoom ...........................................................................................14
1. Change the zooming magnification. ......................................14
4If You Cannot Focus On The Sample Though
The Zooming Body Is At The Highest Position. ......................... 15
IV Using Accessories ........................................................................16
1Reticles ........................................................................................16
2AL ERG (ERGO) Auxiliary Objective ....................................... 17
3SM-S4L 4 X 4 Stage L ................................................................ 18
4Halogen Illuminators ................................................................... 19
1. Lamp ......................................................................................20
2. Attaching to the stand (only for G-LS)..................................21
3. Arm for halogen illuminators ................................................21
4. Power supplies ....................................................................... 23
5. Lighting area adjustment (only for G-LS) .............................25
6. Attaching filters (only for C-DSLS) ...................................... 25
Antistatic Feature ............................................................................... 25
Airtight Feature .................................................................................. 25
Table 1: Total Magnification and Real Field ...................................... 26
Table 2: Observable Sample Heights .................................................27

8
INomenclature
The illustration shows the combination of SMZ645 zooming body,
C-W10X eyepieces and C-PS plain focusing stand.
Eyepiece
10X, 15X, 20X and 30X
eyepieces are available.
Diopter ring
Refer to “1-3. Adjust the
diopter.” on p.12.
Zooming body
Arm
Outside diameter of the part
to be mounted on the arm:
ø76
Groove for mounting
accessories
Holds the Fluorescent
ring illuminator, Fiber
optic ring illuminator
and the like.
Outside diameter: ø60
Screw for mounting
accessories
Holds auxiliary objective
and the like.
The screw on the inside
diameter: M55 × 0.75
Zooming body clamp screw
Fixes the zooming body to
the arm.
Stage plate
The black or the white
side can be chosen
according to the
sample. The antistatic
ESD stage plate is also
available.
Zooming knob
Sleeve
Changes the
magnification of the
sample image.
Refer to “3. Zoom” on
p.14.
Focus knob
Refer to “2. Focusing”
on p.13.
Arm fixing screw
Holds the arm. The
position of the arm can
be chosen from either
high or low.
Hole for clip
Holds the clip for
retaining the sample.
C-PS Focusing stand
Various stands
available.
Vertical slider

9
I
Screw hole for mounting an
arm for halogen illuminator
Screw holes are located on
both sides of the arm. Holds
the G-EIA flexible arm or
Epi-arm.
Eyepiece inclination
SMZ645: 45°from the
horizontal
SMZ660: 60°from the
horizontal
Screw hole for mounting
6V-10W halogen illuminator
Holds the G-LS 6V-10W
halogen illuminator.
Hexagonal wrench
hanger
Holds the supplied
hexagonal
wrenches.
Zooming knob clicks ON/OFF screw
(inside)
Refer to “ON/OFF of the
Zooming Knob Clicks” on p.14
Hexagonal wrench
(Large)
Pillar
Supplied with the
focusing stand for
changing the
height of the arm.
Ground jack
Refer to “Antistatic
Feature” on p.25.
Hexagonal wrench
(Small)
Supplied with the
zooming body for
switching on and
off the zooming
knob clicks.

10
II Assembly
1Place the stand on the level surface.
2Mount the stage plate.
Fit the stage plate into the stand base while
pushing it against the rim in the direction
shown by the arrow in the illustration.
3The stand arm can be lowered. (If you
do not need to lower the arm, jump to
step 4.)
Use the hexagonal wrench (large - supplied
with the focusing stand) to loosen the arm
fixing screw. Reattach the arm using the
screw hole at the lower side of the vertical
slider. The arm will become 55 mm lower
than its original position. Before tightening
the screw, make sure that the two pins on the
arm fit in the grooves on the vertical slider.
4Mount the zooming body.
Lightly tighten the zooming body clamp
screw to hold the zooming body on the arm.
Note) Do not over-tighten the zooming body
clamp screw since it may cause
damage to the instrument.
Groove
Pin

11
II
5Insert the eyepieces into the eyepiece sleeves.
Be sure that it is inserted all the way until it touches the end of the sleeve.
Note) When inserting the 10X eyepiece, assure that it touches the end of
the sleeve, because the rubber cover of the 10X eyepiece will
obstruct the view of the sleeve end. When inserting, hold the rubber
cover not the diopter ring. If not, a failure could result.
6The hexagonal wrenches can be stored in the rear of the pillar.
Refer to the figure on p.9.

12
III Usage
1Preparations For Observation
1Adjust the torque of the focus knob.
Adjust the torque of the focus knob so as not
to fall down the zooming body on its own
weight.
2Adjust the interpupillary distance.
This adjustment should be performed every
time the observer is changed since the
interpupillary distance differs between
individuals. Adjust the interpupillary distance
so that the view field for each eye is merged
into one. Move while holding each sleeve
with both hands.
3Adjust the diopter.
This adjustment should be performed every time the observer is changed
since the eyesight differs between individuals.
1Turn the diopter rings on both eyepieces to set them at the 0 position
(match the 0 line with the index line).
2Turn the zooming knob to 5X. Focus on the sample using the focus
knob. (Refer to “2. Focusing”.)
3Turn the zooming knob to 0.8X. Peering through the left eyepiece
with the left eye, focus on the sample using the diopter ring on the
left eyepiece. Then, peer through the right eyepiece with your right
eye and focus on the sample using the diopter ring on the right
eyepiece.
4Repeat steps 2and 3until the image is kept focused even though the
zooming magnification is changed. This adjustment ensures sharp
image throughout the zooming range.
Increasing the torque.
(To reduce the torque,
turn the knob in the direction
opposite to the arrow.)

13
2Focusing
1Check the working distance.
The distance between the focus plane and the bottom surface of the
zooming body is called “the working distance”. Since the working
distance of the SMZ645 and SMZ660 is 115 mm, the focusing will
become easier if you set the zooming body at the position where its
bottom surface is 115 mm apart from the sample surface.
Refer to the Table 1 (p.26) for the changes in working distances when the
auxiliary objective is attached.
2Focus on the sample.
Turning the left and right focus knobs in the same direction will move the
arm (on which the zooming body is mounted) up and down. Thus you can
focus on the sample.
III

14
3Zoom
1Change the zooming magnification.
Turning the zooming knobs on the left and right side of the zooming body
will change the magnification of the sample image.
■Total Magnification
The zooming knob on the right has the indication of the zooming
magnification. Total magnification can be calculated by multiplying the
eyepiece magnification by the zooming magnification.
Note) When the auxiliary objective is attached, multiply its
magnification as well.
■ON/OFF of the Zooming Knob Clicks
The clicks on the zooming knob can be eliminated.
1Remove the small rubber cap from
the rear of the zooming body.
2Insert the supplied hexagonal wrench
(small) into the screw hole on the rear
of the zooming body.
3Turning the inside screw in the
direction opposite to the arrow
eliminates the clicks (OFF). Turning
the same screw in the direction of the
arrow puts on back the clicks (ON).
4Return the small rubber cap to its
original position.
Zooming
knob

15
III
Usage
4If You Cannot Focus On The Sample Though
The Zooming Body Is At The Highest Position.
When you use the 0.5X auxiliary objective or
observe a tall sample, you may not be able to
focus on the sample though turning the focus
knob to raise the zooming body to its highest
position.
In this case, use the C-ER auxiliary adapter
available as an option.
Note) Attach the auxiliary objective first to
the zooming body, and then mount the
zooming body on the auxiliary adapter.
☞• Refer to the Table 2 on p.27 for the sample heights which may be
viewed.
Auxiliary
adapter
0.5X Auxiliary
objective

16
IV Using Accessories
1Reticles
Your reticles may be attached to the eyepiece.
Once remove the field ring (or lens room of
the 20X and 30X) from the eyepiece. Attach
the reticle with its pattern surface facing down
to the eyepiece and reattach the field ring (or
lens room).
☞• Refer to the Table 1 on p.26 for the size
of the reticle which may be attached.
Note) The 20X and 30X eyepieces adopt the
internal focusing system, and the
primary image has the magnification.
Use the reticle with 1.3X pattern for
20X eyepiece, and with 1.4X pattern
for 30X eyepiece.
A reticle with cross hairs or scaled
cross hairs is available on order for
10X eyepiece.
Separately
sold reticle
Reticle
Pattern
surface
Field ring

17
2AL ERG (ERGO) Auxiliary Objective
Using the AL ERG auxiliary objective allows
you to continuously change the eye level
through a 40 mm range.
1Rotate the milled ring on the AL ERG to
attach it to the zooming body.
2Rotate the focus knobs on the focusing
stand to raise or lower the zooming
body to set to the height best suited for
viewing.
3Rotate the rotation barrel to focus on the
sample.
4To change the eye level more, rotate the
rotation barrel referring to the eye level
scale and focus on the sample using the
focus knobs. One graduation of the eye
level scale is equivalent to 10 mm of the
eye level. The upper the scale, the
higher the eye level.
Note) Changing the eye level will change the magnification. The
magnification is 0.77X at the highest eye level, and 1.06X at the
lowest eye level. To know the exact magnification, calculate it
using the reticle with scaled cross hairs, etc.
IV
Milled ring
Rotation
barrel
Eye level
scale

18
3SM-S4L 4 X 4 Stage L
SM-S4L 4 X 4 stage L may be attached by
using the C-4SA stage adapter. In this case,
use the C-ER auxiliary adapter because the
position of the sample will become higher.
(Refer to “4. If you cannot focus on the
sample though the zooming body is at the
highest position.” on p.15.)
1Remove the stage plate from the stand
base, and attach the C-4SA stage
adapter in place using the fixing screws
supplied with the adapter.
2Sufficiently loosen the stage clamp
screw of the SM-S4L 4 X 4 stage L. Fit
and mount the stage onto the stage
adapter with the side equipped with the
stage clamp screw facing front. Tighten
the stage clamp screw.
4 X 4 stage Stage
clamp screw
Stage adapter
fixing screw
Stage adapter