Omegawave OMEGAFLO FLO-C1 User manual

OA4D9012
Laser Tissue Blood Flowmeter
OMEGAFLO
FLO-C1
USER’S MANUAL

1
CONTENTS
page
CAUTION 2
1. Summary 3
2. Part names and Function 4
3. Connection to Recorder and Operation 8
4. Examples of Measurement 11
5. Theory 13
6. Handling and Cautions 14
7. Specifications 17
8. Guarantee 18

2
CAUTION
1. Turn the laser switch on after the probe was connected.
2. Do not see the laser light through a lens or an optical instrument.

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1. Summary
This laser tissue blood flowmeter, OMEGAFLO FLO-C1, is useful
instrument for measuring tissue blood flow in skin, brain, internal organs, etc. for
animals.
FLO-C1 can measure blood flow continuously and non-invasively, compared
to H2 and Xe clearance method , and is used widely from in fundamental
research to in clinical applications. FLO-C1 uses a semiconductor laser diode
which is hardly affected by the difference of absorption due to oxy-hemoglobin
and deoxy-hemoglobin.
The measurement depth is about 1 mm from the surface in case of using
standard probes. FLO-C1 outputs three blood flow parameters, tissue blood
flow, tissue blood volume and tissue blood velocity, then the hemodynamic
under study in detail can be observed.

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2. Part names and Functions
1) Part names
Front Panel
Rear Panel
○
1Indicator
○
2Parameter selection switch
○
3Mose switch
○
4Laser switch
○
5Optical connectors
Red : Laser output
Black : Receiving
○
6REFLEX
○
7Handle
○
8Time-constant switch
○
9Output 1
○
10 Output2
○
11 AC power input
○
12 Power switch

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2) Part Functions
① Indicator
This LED indicator shows the measurement value of one of tissue blood flow
parameters selected by "Parameter selection switch ②". Also, it shows the
output mode selected by Mode switch ③.
FLO-C1 measures three blood flow parameters, FLOW, MASS and VEL.
FLOW, MASS and VEL. represent tissue blood flow, tissue blood volume and
tissue blood velocity respectively. The ranges of the three parameters are 0-
100, 0-2000 and 0-10.0. All of the parameters can be always monitored
from "Output 1" regardless of the indicated parameter.
Output mode has three modes. "MEA" mode is for measurement, "0" mode
shows 0 (zero) on this indicator and sets the output to 0 (V) and "CAL" mode
shows calibration value and outputs its voltage. The calibration values of
FLOW, MASS and VEL. on Indicator are 10.0, 200 and 1.0 respectively. The
voltage of the all calibration value is 1.0 (V).
② Parameter selection switch
One of the three parameters, FLOW, MASS and VEL., showed on
"Indicator" is selected by this switch.
③ Mode switch
Output mode of "MEA"、"0" and "CAL"is selected by this switch.

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④ Laser switch
Laser power is operated by this switch. Press this switch for ON and the red
light glows.
! Optical connectors
Optical connectors to connect a probe for FLO-C1. The plugs of standard
probes are not specified by RED and BLACK. The plugs of a special probe
are specified by RED and BLACK.
⑥ REFLEX
This bar LED shows the detected laser light intensity on measurement.
The blood flow parameters can be measured when this lamp is green. The
measurement is not affected regardless of the position of the green lamp.
The red lamp on "L" side signifies insufficiency of detected laser light. The
causes of this state are follows:
1) a probe is not put on tissue correctly,
2) laser light is OFF,
3) optical connectors are not connected correctly,
4) a probe is broken,
5) a semiconductor laser is broken.
The red lamp on "H" side signifies over detection of laser light. The causes
of this state are follows :
1) a probe is not put on tissue correctly,
2) tissue under study is highly scattering media.
The solution for measuring highly scattering tissue is use of a specified probe.

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⑦ Handle
Use this handle for carrying FLO-C1.
⑧ Time constant switch
The time-constant of output can be selected by this switch. FLO-C1 has three
states of the time-constant, 0.1(sec), 1(sec) and 3(sec).
⑨ Output 1
The output of three parameters. The voltage of the three parameters is
0-10(V). The output is not affected by "Parameter selection switch".
Measuring value is obtained from 0-10(V) on "MEA" mode.
0 (zero) (V) is generated on each parameter on "0" mode.
1(V) is obtained on each parameter on "CAL" mode.
⑩ Output 2
Detected laser light intensity showed on "REFLEX" is obtained from this
output. The voltage is 0-10(V).
⑪ AC power input
Insert 3P power cord.
⑫ Power switch
This switch is used for "ON-OFF".

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3. Connection to Recorder and Operation
1)Connection to a recorder
(1) Insert the plugs of a probe into the optical connectors of FLO-C1 and screw
the coupling of the plugs to the right by one hand holding the black rubber
boots not to turn by the other. Insert the plugs slowly and in a straight line.
Do not insert the plugs slantwise by force.
Top view
FLO-C1 Optical connector Probe plug
Hold this black rubber boots not to turn,
and screw this coupling.
FLO-C1 has laser incidence and receiving optical connectors. The probe not
being colored has no side. Both plugs of the probes can be inserted into
either side of the optical connectors. Insert the red plug of the probe into the
red mark connector, and the black plug into the black connector if the probe
connectors are colored.
Also, the red connector has the function of adjusting the output laser power.
The output laser power can be lower turning the front ring to CW direction.

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When contact type probe is used, make REFLEX level⑥ being about
center of the LED bar graph using this adjusting ring.
Incidence connector
Adjusting ring
(2) Turn "LASER" switch on. The red lamp is lighted.
(3)Calibration
① Set the full scale of a recorder at 10 (V) using the range dial of the recorder.
② Press "MODE" switch and set at "0".
③ Set the pen of the recorder at zero line using the zero null dial. of the
recorder.
④ Press "MODE" switch again and set at "CAL".
FLOW : 10.0 , MASS: 200 , VEL. : 1.00
are obtained from the out put of FLO-C1. The each voltage is 1 (V).
Set the pen of the recorder at an adequate point.

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⑤ Run the chart paper and make the calibration form showed below operating
① to ④ again.
(4) Fix the probe on a tissue, and press "MODE" switch to set at "MEA" .
Measurement starts.
(5) Raise the gain of the recorder if the measuring wave is too small.
(6) Chose the time-constant switch on the rear panel. To obtain averaged
measuring wave, choose longer time-constant.
Once the recorder is adjusted, this "Calibration" section will not be needed.
3) Stop Operating
(1) Stop the chart of the recorder, and turn off "LASER" switch.
(2) Turn off the power switch of FLO-C1.
(3) Remove the probe from FLO-C1 slowly. Do not pull the plugs out slantwise
by force.
(4) Cap the optical connectors of FLO-C1.
(5) Cap the plugs of the probe and keep it in the probe case.

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Measuring depth: about1mm from the surface

4. Example of Measurement
1) Probe set up
(1) Prepare FLO-C1 and the recorder following "3. Connection to Recorder and
Operation". (page 6-8)
(2) Set the time-constant switch on the rear panel at 0.1 sec .
(3) Fix a probe on tissue under study like the figure below.
Confirm "REFLEX" position is on any green lamp.
Measurement volume is about 1 mm hemisphere, and the depth is about 1
mm from the surface.

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

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



5. Theory
1) Laser tissue blood flowmetry
As laser light illuminates a tissue containing erythrocytes (red blood cells),
the light is diffused by the tissue. Generally, the volume fraction of the
erythrocytes in the tissue is small ; therefore, most of the photons are only
scattered by static tissue elements and, in the case of short optical path
length, a small number of photons are scattered by the erythrocytes once, or
at most a few times, during the scattering process. The light scattered from
the static tissue elements does not shift in frequency, but the light scattered
from the moving erythrocytes does shift in frequency, and is dependent on
the velocity. As tissues are highly scattering materials in near infrared
region, the photons are scattered many times by static tissue elements before
colliding with the erythrocytes. Therefore, the incident directions of the
photons with respect to the erythrocytes are random. The signal output of a
photo-detector broads on the frequency axis because of this randomness,
and spectral characteristics of the signal depend on the velocity and number
density of the erythrocyte in the tissue. (Figure below)
From the spectral characteristics, tissue blood flow (FLOW), tissue blood
volume (MASS) and tissue blood velocity (VEL.) can be obtained by the three
equations.
FLOW =K1∫ωP(ω)dω / I2, MASS =K2∫P(ω)dω / I2, VEL=FLOW / MASS

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6. Handling and Cautions
1) Pay attention to the next matters.
(1) Keep FLO-C1 out of water.
(2) Set FLO-C1 on the place where air pressure, temperature, humidity, wind,
sunlight, dust, and air containing salt and sulfur do not cause bad condition.
(3) Pay attention to inclination, vibration and shock.
(4) Keep FLO-C1 out of a storeroom of chemicals and the place where gas
generates.
(5) Pay attention to the voltage, frequency and power of the power source
which FLO-C1 is connected to.
(6) Connect the ground correctly.
2) Pay attention to the next matters before using FLO-C1.
(1) Examine switch workings and meters, and confirm FLO-C1 working.
(2) Confirm the ground being completely connected.
(3) Confirm all the cables being connected correctly and completely.
(4) Pay attention to using another instruments together.
3) Pay attention to the next matters in use of FLO-C1.
(1) Do not use over the time for diagnosis and medical treatment.
(2) Always keep watch on FLO-C1 and a patient being not unusual.
(3) Take proper measures, like turning off FLO-C1, if something is unusual on
FLO-C1 and a patient
(4) Keep patients out from FLO-C1.
(5) Do not look at the laser light generated from FLO-C1 directly and through
a lens .
(6) Probes are made from glass fibers, and they are hardly repaired.

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


Pay attention to the next matters for probes.
① Do not scrub the tip of probes.
② Do not bend into a circle of under 10 mm radius.
③ Do not step on probes.
④ Keep the tip and plugs clean.
⑤ Do not insert and pull the plugs slantwise by force.
⑥ Use under 60℃ sterilization.
4) Pay attention to the next matters after using FLO-C1
(1) Turn off the power switch after restoring the condition of before using
FLO-C1 along the process.
(2) Do not pull the cables by force.
(3) Pay attention to keeping.
① Keep FLO-C1 out of water.

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② Set FLO-C1 on the place where air pressure, temperature, humidity, wind,
sunlight, dust, and air containing salt and sulfur do not cause bad
condition.
③ Pay attention to inclination, vibration and shock.
④ Keep FLO-C1 out of a storeroom of chemicals and the place where gas
generates.
(4) Put belongings, cables and probes in order after cleaning.
(5) Clean Flo-C1 for the next using.
5) When FLO-C1 is out of order, do not handle. Leave it to a
specialist.
6) Do not remodel FLO-C1.
7) Maintenance
(1) Inspect FLO-C1 regularly.
(2) Confirm FLO-C1 being in order before using it having not used for a long
time.

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7. Specifications
Environmental range for use and keeping
Temperature range
10 〜 35℃
Humidity
30 〜 90% (non-condensing)
Temperature for keeping
-20 〜 60℃
Humidity for keeping
10 〜 90% (non-condensing)
Atmospheric pressure
500 〜 1060 hPa
Laser
Semiconductor laser、Wavelength : 780nm
Under 2mW at the probe end
Class 1 : IEC60825
Sampling Volume
about 1mm hemisphere under a probe
Depth : about 1mm from tissue surface
Signal band width
24Hz -24kHz
Measuring parameters
1)Tissue blood flow(FLOW) : 0-100.0
2)Tissue blood volume(MASS) : 0-1999
3)Tissue blood flow velocity(VEL.) : 0-10.00
Accuracy
+/- 5% (FLOWMETER, based on our standard FLO-C1)
+/- 10% (for standard type probes, based on our standard
probe)
Time-constant
0.1,1 and 3 sec
HDD or SSD
500 GB or more
Input Voltage
AC100 V– 220 V, 50 / 60Hz, 20VA
Size (mm)
W257、H70、D322 (W×H×D)
Weight (kg)
Weight: 4.5

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8. Warranty
1)FLO-C1
Repairs are free within one year after the purchase of FLO-C1 except the
next cases.
(1) Malfunction and damage due to incorrect using and inattention.
(2) Malfunction and damage due to a nature calamity and a fire.
2)Probes
Probes are treated as like consumption articles, and the cost of repair is
according to the table below at the first time.
after purchase cost of repair
within 6 months under 50% of the purchase price
within one year under 70% of the purchase price
after one year not provided
OMEGAWAVE, INC
2-20-3 KATAMACHI
FUCHU, TOKYO, JAPAN
OMEGAWAVE, INC.