Optigene Genie II User manual

®
Genie II
USER MANUAL
(Instrument Software Version v1.18)

Unit 5, latchford Road
Horsham
West Sussex
RH13 5QR
Tel: [phone]
Fax: [phone]
www.optigene.co.uk
[email protected]
Information in this document is subject to change without notice.
OptiGene Ltd. assumes no responsibility for any errors that may appear in this document. This document is believed
to be complete and accurate at the time of publication.
In no event shall OptiGene Ltd. be liable for incidental, multiple, or consequential damages in connection with or
arising from use of this document.
Genie® II and its design is a registered trademark of OptiGene Ltd. or its subsidiaries in the UK and other countries.

Table of Contents
TA LE OF CONTENTS .......................... I
SAFETY NOTICES ................................ 3
SUPPORT ............................................ 5
How to obtain support....................... 5
Supported consumables .................... 5
OX CONTENTS .................................. 6
SITE PREPARATION ............................ 7
How to set up Genie® II ..................... 7
CONNECTIONS ................................... 8
Opening & Closing the Lids ................ 8
Inserting Tubes .................................. 9
attery ............................................... 9
attery monitor ............................... 10
Screen rightness Control ............... 11
EM EDDED SOFTWARE ................... 12
Genie®II Welcome Screen................ 12
Main Menu ...................................... 13
Initial set up ..................................... 13
Utilities ............................................. 14
Touchscreen calibration .............. 14
Set Time and Date ........................ 15
Turn off ........................................ 15
Run ................................................... 16
Profile Screen ............................... 16
To create a new profile ................ 16
To load a saved profile ................ 17
ACTIVE ............................................. 20
Profile .......................................... 20
Temperature ................................ 20
Amplification ............................... 21
Anneal .......................................... 21
Results ......................................... 22
A/ ................................................... 22
NOTES .............................................. 23
VIEW ................................................ 23
CONNECTING TO A COMPUTER ....... 27
Genie® II Software updates ............. 31
Via PC ........................................... 32
Via Instrument (v1.17 & Later) .... 34
PC SOFTWARE .................................. 36
Welcome Screen .............................. 36
Quick start buttons .......................... 36
Setting up a run ............................... 37
Profile Screen ............................... 37
Notes ........................................... 37
Assays .......................................... 38
Wells ............................................ 38
Profile .......................................... 38
Graphs ......................................... 40
Peak Classification ....................... 40
Genotype ..................................... 41
Run ............................................... 41

Observing a run ................................ 42
Profile ........................................... 42
Isothermal .................................... 42
Isothermal Ratio .......................... 42
Anneal .......................................... 43
Anneal Derivative......................... 43
Peaks ............................................ 44
Results .......................................... 44
Analysis and Graph Options ............. 45
Genie® II Graph Options .............. 45
Opening previous run files ............... 47
Additional features .......................... 47
TECHNICAL SPECIFICATION .............. 50

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SAFETY NOTICES
Please read the following notices carefully before
using Genie® II.
The equipment supplied has been designed to be completely safe to use. However to avoid any
risk to the safety of the equipment, operator, or anybody in the vicinity of as the equipment,
please read this chapter before unpacking and using the instrument. If there is any doubt as to
the correct use of the equipment contact the vendor.
Notices
Using the instrument in a manner not specified by OptiGene may result in
personal injury or damage to the instrument.
Always ensure that the surface on which the instrument is placed is level and
stable and will not cause the instrument to topple over. Ensure that the surface
is suitable for the weight and size of the instrument. If the instrument is
dropped it may cause harm.
The instrument should never be lifted by its covers. Always ensure that the base
or sides are used as the lifting point.
The instrument is electrically powered. Please ensure that the correct voltage
settings have been applied before applying power to the instrument. If in doubt
consult a qualified electrician. The instrument has a rating label affixed to the rear.
Please consult this if needed.
Chapter
1

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Always disconnect the equipment before moving or removing any guards or
covers. Switch off at the mains, remove the mains plug from the wall socket and
remove the cable from the inlet socket on the rear.
While every effort has been made to protect the inside of the instrument
against splashes, the instrument carries no IP rating. If fluids are spilt on the
instrument they may cause damage and cause an electrical hazard.
If a spill occurs, remove power from the instrument. Do not touch the
instrument or any fluid flowing from it while it is connected to the mains supply.
Always follow local health and safety guidelines.
Normal safe local operating standards should be applied at all times. The warnings above are
for guidance only. Please consult the instrument supplier if there is any doubt.
Disconnection Method
Genie® II is disconnected by removal of incoming mains power source to the
unit. Following disconnection the unit should be left for a period of at least 5
minutes before any internal assemblies are removed or examined.
When in use the heating blocks and heated lid are hot, so allow to cool before
touching the surfaces.
Safe removal of fluids from Genie® II will depend on the chemistry used. This will
also require knowledge of the fluids used in the system to adhere with local health
and safety and COSHH regulations. If in doubt, consult the person responsible for the
equipment in the laboratory.

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SUPPORT
HOW TO O TAIN SUPPORT
For the latest services and support information go to http://www.optigene.co.uk/support.htm
IMPORTANT! When directed to do so, contact OptiGene Ltd. to schedule maintenance
or calibration of a Genie® II instrument.
IMPORTANT! If a Genie® II instrument is kept in a very cold environment, the battery
will not begin charging until the internal temperature has reached 15°C.
SUPPORTED CONSUMA LES
IMPORTANT! Genie® II uses a proprietary tube strip that maximises optical and
thermal efficiencies. Other tubes and strips will not fit.
IMPORTANT! Forcing non-supported consumables will cause damage to the
instrument and invalidate the warranty.
IMPORTANT! The shape of the tubes is such that they will only fit in one way round.
The locating pins on the block have corresponding holes in the strips.
Catalogue number Description
ISO-001 Isothermal Master Mix
OP-0008-50 Genie® II tubes pack size 50 strips
OP-0008-500 Genie® II tubes pack size 500 strips
Chapter
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OX CONTENTS
The following is a list of contents in the box for Genie® II:
• Genie® II instrument
• Power supply
• Power lead
• US connection lead
• US memory stick containing Genie® II software and this manual as a ‘.PDF’ file
• 20 x Genie® II tubes (optional)
• Sample preparation/cooling blocks (optional)
Chapter
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Chapter
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Version 1.13
SITE PREPARATION
HOW TO SET UP GENIE® II
The laboratory bench should be level and stable. The instrument should be placed centrally on
the lab bench and the surfaces surrounding the instrument must be clear of obstructions at all
times.
Care must be taken not to unduly restrict the air at the front of the instrument and the outlet
vents at the rear. Restricting airflow may impede operation and could affect performance.
Electrical points should be close to the instrument to avoid injury from trailing wires.
It is recommended that the instrument is kept away from sinks and other wet areas. Genie® II
is an electrical instrument and care should be taken not to operate if there is a risk of water
damage.
Chapter
1
Cha
pter
4

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CONNECTIONS
Genie® II is ready to use straight out of the box without any connections as it can be used as a
standalone instrument and has an internal battery. However to charge the battery or to use
Genie® II with a computer, some connections must be made.
Connect the power supply plug into the back of the instrument and then attach the power
cable to the supply.
Located to the rear of the instrument is an on/off power button, switch to on and Genie® II will
power up and progress through its checks.
OPENING & CLOSING THE LIDS
Gently lift the lid and it should open upwards. Close the lid by lowering gently.
Care must be taken to ensure that objects are not obstructing the lid when trying
to close it and under no circumstances should the lids be forced open or closed.
POWER IN
US