PerkinElmer Lambda 365 User manual

09931286E
PerkinElmer, 710 Bridgeport Avenue,
Shelton, CT 06484-4794, U.S.A
Produced in the USA.
Lambda 365 6-Position Peltier Controlled Cell Changer,
Controller Installation Instructions
This instruction sheet describes the installation of this accessory which is used with the Lambda 365
Spectrophotometer.
NOTE:
Read these instructions before you install this accessory.
Contacting PerkinElmer
Supplies, replacement parts, and accessories can be ordered directly from PerkinElmer, using the part
numbers.
See our website:
http://perkinelmer.com
PerkinElmer's catalog service offers a full selection of high-quality supplies.
To place an order for supplies and many replacement parts, request a free catalog, or ask for
information:
If you are located within the U.S., call toll free 1-800-762-4000, 8 a.m. to 8 p.m. EST. Your order will be
shipped promptly, usually within 24 hours.
If you are located outside of the U.S., call your local PerkinElmer sales or service office.
Features
•Full software control
•Liquid cooling system
•N2gas purging available
Figure 1 6-Position Peltier Controlled Cell Changer, Controller (with heated
reference) [P/N: N4101029] or Peltier Temp Unit (Multi) w/o Reference
Heating [P/N: N4101008]

09931286E
2
Dimensions and Specifications
Peltier Controller
Physical Characteristic Specification
Power 100-240 VAC, 50/60 Hz, 300W, Free Voltage
Temperature Range -5 to 100°C (Maximum Internal Temperature)
Maximum Ambient Operating Temperature 40°C
Dimensions 310 (W) x 410 (D) x 275 (H) mm
(12.2 (W) x 16.1 (D) x 10.8 (H) in)
Weight 20.0 kg (44 lb)
Coolant Volume 2 L
N2Gas Available 100 psi (6.9 bar)
Liquid Cooling System
Magnetic Stirrer Control Available
Emergency Power Off Switch Available
6-Position Peltier Controlled Cell Changer (with and without heated reference)
Physical Characteristic Specification
The number of Sample Cells 6
The number of Reference Cell 1 (with or without heated reference)
Dimensions 135 (W) x 384 (D) x 143 (H) mm
(5.3 (W) x 15.1 (D) x 5.6 (H) in)
Weight With heated reference: 3.4 Kg (7.5 lb)
Without heated reference: 3.1 Kg (6.8 lb)
Temperature Accuracy ±0.15°C
Temperature Precision ±0.1°C
Temperature Stability ±0.1°C
Ramping Time (from 0 to 100°C) 5 minutes

09931286E
3
Safety Warnings
WARNING
When this label is attached to an instrument it means refer to the
manual.
Lorsque cette étiquette est attachée à un instrument, il est nécessaire
de voir le manuel.
WARNING
There is risk of receiving a fatal electric shock if the fuses are replaced
with the power cord connected.
Il y a un risque d’électrocution si les fusibles sont remplacés tandis que
le cordon d'alimentation est encore branché.
See the following figure for the location of warning labels on the back of the instrument:
Warning
FUSE
Avertissement
FUSE
Warnin
g
To prevent electrical shock,
do not open cover.
Refer all servicing to qualified
personnel.
Avertissement
Afin d’éviter tout risque
d’électrocution ne pas enlever ce
couvercle.
Faire appel au service Après-Vente.
Warnin
g
For continued protection
against electrical shock,
replace with same type and
ratings of fuse.
Avertissement
Pour assurer la protection
contre le risque de choc
électrique, remplacez le fusible
seulement avec le même type
et la même valeur nominale.

09931286E
4
Configuration of Peltier Temp. Control Unit (Multi)
6-Position Peltier Controlled Cell Changer (with heated reference or without heated reference)
Peltier Controller
Power Cord, 3ea
Interface Cable (RS-232 and USB to RS-232), 1each
Spare Fuse (AC 250V T5AL), 2ea
Temperature Probe, 1ea
Magnetic Stirrer, 6ea
Coolant Hose, 1ea
Coolant, 2ea
Waste Basket for Coolant, 1ea
Waste Hose for Coolant, 1ea
Phillips round head screw with washer, 2ea
Description
6-Position Peltier Controlled Cell Changer (with heated reference or without
heated reference)
Figure 2-1 Lambda 365 6-Position Peltier Controlled Cell Changer (with heated
reference)

09931286E
5
Figure 2-2 Lambda 365 6-Position Peltier Controlled Cell Changer (without heated
reference)
a. Interface connector between 6-Position Peltier Controlled Cell Changer and Peltier Controller
b. Coolant Inlet/Outlet Quick Coupler
c. N2Gas fitting
d. Interface slot for Temperature Probe
e. Cell Lifting knob for sample cells
f. Cell Lifting knob for reference cell
g. Phillips round headscrew with washer
Used to fix a cell holder, a base plate or a front plate for Lambda 365.
Spare screws (2ea) are enveloped with the accessory.
Figure 3 Phillips round headscrew with washer (M4 *12L)
h. Temperature Probe
Figure 4 Temperature probe

09931286E
6
Peltier Controller
Front View of Peltier Controller
Figure 5 Front view
a. Manual touch pad with Display: Temperature Control Unit (Heating/Cooling Block).
b. LED Indicators: Display the status of the operation of Coolant Circulation, Fan, Pump, etc.
(If there is any problem in the components, Red LED will flash with beep alarm.).
c. Manual ON/OFF button: As the Manual ON/OFF button is ON, the temperature can be
controlled manually by a) Manual touch pad with Display.
NOTE:
Manual operation is used only for the manufacturer so it should be turned off during the measurement.
d. N
2
gas ON/OFF button: It is used for using N
2
gas to remove the frost from the heating
and cooling.
e. Stirrer ON/OFF button: It is used for controlling of the stirring.
f. Stirrer Speed Controlling Knob: Control the stirring speed (rpm).
g. Emergency switch: Stop all operations in the emergency situation.
NOTE:
During the operation, Emergency switch should be turned off.
h. Coolant Inlet

09931286E
7
Rear View of Peltier Controller
Figure 6 Rear view
a. RS-232 port
b. AC Power: Main Power ON/OFF
c. Fuse: AC socket + Fuse Holder
d. Interface: Interface cable for connecting with 6-Position Peltier Controlled Cell Changer
e. Air Vent Manual button: It is used for removing the air in the coolant hose.
f. Buzzer: It makes an alarm sound. If there is any problem before the measurement, Buzzer
beeps 6 times at every 10 sec. If the malfunction occurs during the operation, Buzzer beeps
twice at every 10 sec.
g. Quick Coupler of Coolant lnlet/Outlet
h. Quick Coupler of Coolant Drain
i. N2Gas Inlet/Outlet ports
NOTE:
N
2
gas is not always required [Tube for N
2
gas is not supplied with the accessory].
j. Flow Gauge: Indicator of the flow rate of N2gas.

09931286E
8
Installation
1. Prepare the Lambda 365 and Peltier Controller in a location that is compatible with the required
environmental conditions for the operation.
2. Open the lid of the coolant inlet on top of the Peltier Controller.
Figure 7 Lid of coolant inlet
3. Fill up the liquid coolant.
CAUTION
ATTENTION
Liquid coolant should be used as refrigerant. Do not use any water (tab water,
DI, etc.). If water is used as refrigerant, the Peltier Controller’s components might
be corroded and its performance may be deteriorated.
Use any kind of liquid coolant of normal grade that is available. (e.g. liquid coolant
including Distilled Water 70 wt%, Propylene Glycol 27 wt%, Additives 3 wt%).
Le liquide de refroidissement doit être utilisé comme réfrigérant. N'utilisez jamais
d'eau (eau du robinet, eau déminéralisée, etc.) L'eau risque en effet de corroder
les composants du contrôleur Peltier et de réduire les performances de ce
dernier.
Utilisez un liquide de refroidissement du commerce de qualité normale (par
exemple, un liquide de refroidissement à base d'eau distillée à 70 % M/V, de
propylène glycol à 27 % M/V et d'additifs à 3 % M/V).
NOTE: Keep the amount of liquid coolant to the level where the indicator is located between the ‘L’(low) and
‘H’(high) mark in the scale on the left side of the Peltier Controller. When the coolant is running short for
the operation, LED of Low Water Level is changed red with alarm sounding.
4. Connect the power cord and communication cable of the Lambda 365. DO NOT turn on the power
of the instrument!
CAUTION
ATTENTION
Make sure the instrument is turned off while installing this accessory.
Assurez-vous que l’instrument est éteint lors de l’installation de cet accessoire.

09931286E
9
5. Remove the two Phillips round head screws with washer (M4*12L) to disassemble the existing cell
holder and base plate.
Figure 8 Location of Phillips round head screws with washer
6. Pull out the cell holder and base plate by hand.
Figure 9 Pulling out the cell holder and base plate
7. Insert the 6-Position Peltier Controlled Cell Changer in the sample compartment.
Figure 10 Inserting the 6-Position Peltier Controlled Cell Changer

09931286E
10
8. Gently press the 6-Position Peltier Controlled Cell Changer to connect the communication port (male)
under the bottom of the 6-Position Peltier Controlled Cell Changer to the port (female) in the
sample compartment.
Figure 11. Connecting the communication ports
9. Fix and tighten the 6-Position Peltier Controlled Cell Changer with the Phillips round head screws.
or
Figure 12 Location of screws (with heated reference or without heated reference)

09931286E
11
10. Connect the accessory interface cable of the Peltier Controller to the interface connector of the 6-
Position Peltier Controlled Cell Changer.
Figure 13 Connecting the accessory interface cable of the Peltier Controller
11. Connect the coolant inlet/outlet and N2gas tube between the Peltier Controller and the
6-Position Peltier Controlled Cell Changer.
Figure 14 Connecting the coolant inlet/outlet and N2gas fitting
NOTE:
N
2
gas is not always required. If no gas is applied, user does not have to connect N
2
gas tube.
12. Open the lid of Interface slot for Temperature Probe by hand.
Figure 15 Opening the lid of Interface slot

09931286E
12
13. Connect the Temperature Probe to the Interface slot for Temperature Probe.
Figure 16 Connecting the temperature probe
14. Stuff the probe line into the cell compartment and close the lid of the Interface slot for
Temperature Probe.
Figure 17 Stuffing the probe line into the cell compartment
15. Connect the Peltier Controller with the PC via the RS-232 or USB to RS-232 cable.
Figure 18 Connecting the Peltier Controller with the PC

09931286E
13
16. Connect the power cord to the Peltier Controller.
Figure 19 Connecting the power cord
17. Turn on the AC power switch of the Lambda 365 and the Peltier Controller.
CAUTION
ATTENTION
The air vent manual button should be off before turning on the main power of the
Peltier Controller and also during the operation since it could make a trouble for
the communication between Lambda 365 and Peltier Controller.
Le bouton d'actionnement manuel de la ventilation doit être désactivé avant que
le contrôleur Peltier ne soit mis sous tension et pendant le fonctionnement de ce
dernier afin d'éviter qu'une erreur de communication entre le Lambda 365 et le
contrôleur Peltier ne se produise
Figure 20 Turn on the AC power switch
18. Check that the Power LED is on as a blue light.

09931286E
14
Figure 21 Location of Power LED
CAUTION
ATTENTION
If you hear the alarm sound during the operation, push the emergency
switch to stop all functions immediately and check the status of Peltier
Controller carefully. Then, turn the switch to the right again when
everything is solved properly and the functions will be on again.
Si l'alarme se déclenche en cours de fonctionnement, appuyez sur
l'interrupteur d'urgence pour arrêter immédiatement toutes les fonctions
et contrôlez soigneusement l'état du contrôleur Peltier. Ensuite, tournez à
nouveau l'interrupteur vers la droite une fois la situation rétablie afin de
restaurer les fonctions
Figure 22 Location of Emergency Switch
NOTE:
Peltier controller can be compatible with various peltier cell holders (single or Multi). Wwhenever you
exchange the existing peltier cell holder to another one, you should perform the “Auto Tuning” setup
referring to chapter Peltier Controlled Auto Tuning Setup (p33).
19. Turn off the power after the experiment.
How to Drain the Coolant From the Peltier Controller
a. Push the Air Vent Manual button to be switched on the rear panel of Peltier Controller before
operation.
b. Connect the waste hose to the Coolant Drain port on the rear panel and prepare a waste
bucket.
c. Turn on the power of Peltier Controller, and then coolant will be drained automatically.

09931286E
15
d. Once draining is complete, turn off the power of Peltier Controller.
Setting USB to RS-232 Cable
NOTE: When using the RS-232 cable, the COM Port is set automatically.
When using the USB to RS-232 cable, USB to RS-232 Driver has already been installed when installing
the Lambda 365 and UV Express software, user does not need to install it again.
When the communication by the USB to RS-232 converter is not established, change the port setting as
follows;
1. Select My Computer →Properties.
2. Select Device Manager.

09931286E
16
3. Select Ports (COM & LPT) to expand the listing. These are the devices currently connected to the
COM ports. USB Serial Port (COMx) is listed when the driver installation is completed
successfully.
4. Double click on the USB Serial Port (COMx) of the Ports (COM & LPT).
5. Select the Port Settings tab and select the Advanced… button.

09931286E
17
6. Change the parameter values as shown below.
7. Select OK after checking the changed parameter values.
8. If the Peltier controller fails to communicate with the PC, change the COM Port Number by the
following steps.
9. Open Advanced Setting for COMx window again by repeating the steps 1 to 6.

09931286E
18
10. Select the COM Port number list to expand it and change the COM port number to another one
which is not in use from COM 1 to COM 10.
11. Make sure that the changed COM Port Number is applied, and select OK.
12. After the port setting is changed, restart the computer.

09931286E
19
Measurement
Thermal Denaturation Mode
1. Install the 6-Position Peltier Controlled Cell Changer referring to the chapter Installation.
2. Close the sample compartment cover and turn on the instrument.
NOTE:
Before System Self Test is performed, the 6-Position Peltier Controlled Cell Changer should be installed.
Otherwise, the instrument can be damaged electrically and do not operate properly.
NOTE: Start the System Self Test after warming up the system for at least 20 minutes.
3. Double-click on the UV Express folder and select Thermal Denaturation mode.
4. Select OK after finishing the System Self Test.
5. Select New to open a new window and select OK.
NOTE:
When the 6-Position Peltier Controlled Cell Changer is connected for the first time, you need to correct
the cell position. Calibrate the beam position of the 6-Position Peltier Controlled Cell Changer referring to
the chapter Calibration of Multi-Cell Position (p30).
6. Open the method in main menu or mode icon on the left side of the main window. Set parameters
for Experiment.

09931286E
20
a. Y Unit: Select one of the display unit: Absorbance, Transmittance, Reflectance or Energy.
b. SBW (nm): Select bandwidth. There are five bandwidths selectable: 0.5, 1, 2, 5 or 20 nm.
c. Tm Method: Select a method for determining melting temperature. Options include: 1st
derivative and Average.
d. Equation Name:
Enter the name of the formula. %G-C is entered as default, of which equation is formulated in
Equation Expression.
e. Equation Expression
The default equation for the calculation of %G-C base pairs is:
%G-C=2.44 * (Tm - 81.5 - 16.66 * log(M) + 500/K)
Where M is the molarities of salt in mol/L, K is the DNA base pair length. %G-C will be
calculated with estimated Tmand input values, Molarities (M) and length of DNA (K).
f. DNA Pair Length (K)
Enter the DNA base pair length. This value will be used calculating %G-C.
g. Calculation Start Temperature (°C)
Enter the start temperature for calculating the Tmvalue.
h. Calculation End Temperature (°C)
Enter the end temperature for calculating the Tmvalue.
i. Molarity (mol/L)
Enter the salt molarities of the solution [mol/L]. This value will be used in calculating %G-C.
7. After setting parameters for Experiment and Lamp, click the Accessory tab. And select Multi-
Cell Peltier.