PerkinElmer MultiScope System Service manual

MultiScope System Microscope
User's Reference

Release History
Part Number Release Publication Date
L1050041 B December 2013
Any comments about the documentation for this product should be addressed to:
User Assistance
PerkinElmer Ltd
Chalfont Road
Seer Green
Beaconsfield
Bucks HP9 2FX
United Kingdom
Or emailed to: [e-mail]
Notices
The information contained in this document is subject to change without notice.
Except as specifically set forth in its terms and conditions of sale,
PerkinElmer makes no warranty of any kind with regard to this
document, including, but not limited to, the implied warranties of
merchantability and fitness for a particular purpose.
PerkinElmer shall not be liable for errors contained herein for incidental
consequential damages in connection with furnishing, performance or use of this
material.
Copyright Information
This document contains proprietary information that is protected by copyright.
All rights are reserved. No part of this publication may be reproduced in any form
whatsoever or translated into any language without the prior, written permission of
PerkinElmer, Inc.
Copyright © 2013 PerkinElmer, Inc.
Produced in the UK.
Trademarks
Registered names, trademarks, etc. used in this document, even when not
specifically marked as such, are protected by law.
PerkinElmer is a registered trademark of PerkinElmer, Inc.
MultiScope is a trademark of PerkinElmer, Inc.
Spectrum is a trademark of PerkinElmer, Inc.
Fluorolube is a registered trademark of the Hooker Chemical Corporation.
Nujol is a registered trademark of the Esso Standard Oil Company.

Contents
Contents .................................................................................................3
Introduction .........................................................................................5
Introduction to this Guide.........................................................................6
Related Documents..................................................................................7
What am I Expected to Know? ..................................................................8
Conventions Used in this Manual ...............................................................9
Warning Signs on the Instrument............................................................ 12
Warnings and Safety Information .....................................................15
Summary .............................................................................................. 16
General Operating Conditions.................................................................. 17
Environmental Conditions ....................................................................... 18
Electrical Safety ..................................................................................... 19
Location and Ventilation ......................................................................... 21
Laser Safety Regulations......................................................................... 22
Laser Labels and Laser Apertures ............................................................ 24
Warning Label Near the Lamp Housing .................................................... 26
Lifting the Microscope............................................................................. 27
EMC Compliance .................................................................................... 28
System Requirements............................................................................. 29
Overview of the MultiScope System Microscope...............................31
Features of the MultiScope System Microscope ......................................... 32
Applications........................................................................................... 35
The Optical System ................................................................................ 36
Specifications......................................................................................... 42
Getting Ready to Use the Microscope................................................45
Getting Ready to Use the Microscope....................................................... 46
Setting Up the FT-IR .............................................................................. 47
Cooling the MCT Detector....................................................................... 48
Setting Up the Microscope ...................................................................... 51
Viewing the Sample................................................................................ 52
Tutorial: Using the Microscope ..........................................................53
Introduction to the Tutorial..................................................................... 54
Transmittance Spectrum of a Flattened Fiber............................................ 55
Reflectance Spectrum of a Thin Film........................................................ 59
Preparing Samples .............................................................................63
Preparing Samples ................................................................................. 64
Tools for Sample Preparation .................................................................. 65
Sampling Accessories Available as Options ............................................... 66
Common Window Materials..................................................................... 71
Techniques for Preparing Samples........................................................... 72
Special Cells .......................................................................................... 81

Collecting Spectra with the Microscope ............................................83
Collecting Spectra with the Microscope.....................................................84
Positioning the Sample ........................................................................... 85
Collecting a Background Spectrum........................................................... 88
Collecting the Spectrum of the Sample..................................................... 91
If You Must Collect the Sample Spectrum First.......................................... 92
Collecting the Spectrum of a Thick Sample ............................................... 93
Operating the Optional Equipment....................................................95
Operating the Optional Equipment...........................................................96
Visible Polarizer Option ........................................................................... 97
Infrared Polarizer Option....................................................................... 103
Collecting a Spectrum in an Inert Atmosphere ........................................ 107
Applications......................................................................................109
Applications......................................................................................... 110
Reflectance FT-IR Microspectroscopy..................................................... 111
Polymers ............................................................................................. 115
Criminalistics........................................................................................ 117
Biological Materials............................................................................... 118
Product Contaminants........................................................................... 119
Maintenance .....................................................................................123
Maintenance........................................................................................ 124
Selecting the Microscope Voltage and Renewing the Fuse........................ 127
Aligning the MCT Detector .................................................................... 128
Changing the Lamp .............................................................................. 130
Electrical Connections........................................................................... 132
Appendix: WEEE Instructions for PerkinElmer Products............................ 140
Index.................................................................................................. 141

Introduction

6 . MultiScope System Microscope User's Reference
Introduction to this User's Reference
This introduction gives you information about this User's Reference , so you can use
it effectively when learning to operate the microscope.
We recommend that you use it in the following order:
•Read the rest of this Introduction and become aware of requirements and
conventions.
•Read the
Overview of the MultiScope System Microscope .
•Do the setup procedures in
Getting Ready to Use the Microscope .
•Try the tutorial in
Tutorial: Using the Microscope . This gives you experience of
using the microscope to collect both transmittance and reflectance spectra.
•The procedures you use for all your work with the microscope are in
Getting
Ready to Use the Microscope
, Preparing Samples, Collecting Spectra with the
Microscope
and Operating the Optional Equipment.
•
Getting Ready to Use the Microscope contains the procedures you follow at the
beginning of the day's work to make sure the microscope is set up properly.
•
Preparing Samples describes techniques for preparing many types of
microscopic samples. It includes descriptions of the sample preparation tools
provided with, or available for, the PerkinElmer MultiScope System Microscope.
•
Collecting Spectra with the Microscope contains general procedures for
collecting spectra with the microscope.
•
Operating the Optional Equipment describes how to use the optional
equipment that is available for your microscope.
•Further reference information on the microscope is given in
Applications and
Maintenance .
•
Applications gives information on several applications for which the microscope
is particularly useful.
•
Maintenance contains maintenance information. Read through it to see how to
care for the microscope and to learn about the performance checks that we
recommend that you do routinely.
If you find an error in this manual or have any comments or suggestions, please
contact User Assistance at PerkinElmer. Our address is on page 2.

Introduction . 7
Related Documents
Manuals for PerkinElmer FT-IRs
Information on using your FT-IR is found in the manuals that were supplied with it.
Optional equipment
Certain items of the optional equipment for the microscope have their own
manuals. Consult the appropriate manual before attempting to use the equipment.

8 . MultiScope System Microscope User's Reference
What am I Expected to Know?
We assume that you have some familiarity with operating the FT-IR. Specifically,
you must be able to:
•Set the FT-IR to use the external beam;
•Monitor the signal;
•Set the gain;
•Collect background and sample spectra.
This
User's Reference does not give detailed procedures for these operations.
Consult the manual for the FT-IR.

Introduction . 9
Conventions Used in this Manual
Normal text is used to provide information and instructions.
Bold text refers to text that is displayed on the screen.
UPPERCASE text, for example ENTER or ALT, refers to keys on the PC keyboard.
'+' is used to show that you have to press two keys at the same time, for example,
ALT+F.
All eight digit numbers are PerkinElmer part numbers unless stated otherwise.
Notes, cautions and warnings
Three terms, in the following standard formats, are also used to highlight special
circumstances and warnings.
NOTE: A note indicates additional, significant information that is provided with
some procedures.

10 . MultiScope System Microscope User's Reference
CAUTION
We use the term CAUTION to info
rm you about situations that
could result in
serious damage
to the instrument
or other
equipment. Details about these circumstances are in a box like
this one.
D
Caution (Achtung)
Bedeutet, daß die genannte Anleitung genau befolgt werden muß,
um einen
Geräteschaden
zu vermeiden.
DK
Caution (Bemærk)
Dette betyder, at den nævnte vejledning skal overholdes nøje for
at undgå en
beskadigelse af apparatet
.
E
Caution (Advertencia)
Utilizamos el término
CAUTION
(ADVERTENCIA) para advertir
sobre situaciones que pueden provocar
averías graves
en este
equipo
o en otros. En recuadros éste se proporciona información
sobre este tipo de circunstancias.
F
Caution (Attention)
Nous utilisons le terme
CAUTION
(ATTENTION) pour signaler les
situations susceptibles de provoquer de
graves détériorations
de l'instrument
ou d'autre matériel. Les détails sur ces
circonstances figurent dans un encadré semblable à celui-ci.
I
Caution (Attenzione)
Con il termine
CAUTION
(ATTENZIONE) vengono segnalate
situazioni che potrebbero arrecare
gravi danni
allo strumento
o ad altra apparecchiatura. Troverete informazioni su tali
circostanze in un riquadro come questo.
NL
Caution (Opgelet)
Betekent dat de genoemde handleiding nauwkeurig moet worden
opgevolgd, om
beschadiging van het instrument
te
voorkomen.
P
Caution (Atenção)
Significa que a instrução referida tem de ser respeitada para
evitar a
danificação do aparelho
.

Introduction . 11
WARNING
We use the term WARNING to inform you about situations that
could result in
personal injury
to yourself or other persons. Details
about these circumstances are in a box like this one.
D
Warning (Warnung)
Bedeutet, daß es bei Nichtbeachten der genannten Anweisung zu
einer
Verletzung
des Benutzers kommen kann.
DK
Warning (Advarsel)
Betyder, at brugeren kan blive
kvæstet
, hvis anvisningen ikke
overholdes.
E
Warning (Peligro)
Utilizamos el término
WARNING
(PELIGRO) para informarle sobre
situaciones que pueden provocar
daños personales
a usted o a
otras personas. En los recuadros como éste se proporciona
información sobre este tipo de circunstancias.
F
Warning (Danger)
Nous utilisons la formule
WARNING
(DANGER) pour avertir des
situations pouvant occasionner des
dommages corporels
à
l'utilisateur ou à d'autres personnes. Les détails sur ces
circonstances sont données dans un encadré semblable à celui-ci.
I
Warning (Pericolo)
Con il termine
WARNING
(PERICOLO) vengono segnalate
situazioni che potrebbero provocare
incidenti alle persone
.
Troverete informazioni su tali circostanze in un riquadro come
questo.
NL
Warning (Waarschuwing)
Betekent dat, wanneer de genoemde aanwijzing niet in acht wordt
genomen, dit kan leiden tot
verwondingen
van de gebruiker.
P
Warning (Aviso)
Significa que a não observância da instrução referida poderá causar
um
ferimento
ao usuário.

12 . MultiScope System Microscope User's Reference
Warning Signs on the Instrument
Caution, hot surface.
Caution, risk of electric shock.
Caution, laser radiation hazard.
Caution risk of danger.
Refer to accompanying documents in all cases where this symbol
is used to find out the nature of the potential HAZARD and any
actions which have to be taken.

Introduction . 13
FT-IR
The MultiScope System Microscope can be used with several different PerkinElmer
FT-IR spectrometers. In this User's Reference , we have used the term FT-IR to
mean the FT-IR spectrometer that you have connected to the microscope.
Spectrum 100 Series
The MultiScope System Microscope can be used with Spectrum 100 Series
spectrometers.
Spectrum One
The MultiScope System Microscope can be used with Spectrum One spectrometers.
Spectrum GX
The MultiScope System Microscope can be used with Spectrum GX, System 2000,
Spectrum 2000, System 2000R NIR FT-Raman, Spectrum 2000R NIR FT-Raman
FT-IR, and Spectrum GXR FT-IRs. In this User's Reference we have used
Spectrum GX to indicate Spectrum GX, System 2000, Spectrum 2000,
System 2000R NIR FT-Raman, Spectrum 2000R NIR FT-Raman FT-IR, and
Spectrum GXR spectrometers.
Spectrum BX
The MultiScope System Microscope can be used with Spectrum BX, Paragon
1000PC, and Spectrum 1000 FT-IRs. In this
User's Reference we have used
Spectrum BX to indicate Spectrum BX, Paragon 1000PC, and Spectrum 1000
spectrometers.
Spectrum RX
The MultiScope System Microscope can be used with Spectrum RX, or
Paragon 1000 FT-IRs. In this User's Reference we have used Spectrum RX to
indicate Spectrum RX or Paragon 1000 spectrometers.
Spectrum 400 Series
The MultiScope System Microscope can be used with Spectrum 400 Series
spectrometers.
Frontier
The MultiScope System Microscope can be used with Frontier FT-IR spectrometers.

14 . MultiScope System Microscope User's Reference

Warnings and Safety
Information

16 . MultiScope System Microscope User's Reference
Summary
The PerkinElmer MultiScope System Microscope has been designed to comply with
a wide variety of international standards governing the safety of laboratory
equipment. In routine use, the microscope poses virtually no risk to you. If you take
some simple, common-sense precautions, you can make sure that you maintain the
continued safe operation of the microscope.
DO make sure that the microscope is properly connected to the electrical supply; in
particular, make sure that the ground (earth) is securely connected.
DO disconnect the electrical power supply before opening the main cover of the
microscope.
DO keep the microscope dry. Avoid spilling liquid into the microscope. Clean all
external spills immediately. If anything that is spilled enters the main body of the
microscope, switch off the power and call a PerkinElmer Service Engineer.
DO wear safety glasses and protective gloves when you are filling the detector
dewar with liquid nitrogen. Slowly pour the liquid nitrogen into the dewar. Stand
back from the detector during filling since liquid nitrogen may be expelled from the
dewar flask. Use only liquid nitrogen.
DO NOT stare into the laser beam. Laser radiation will be present when the
microscope is connected to an FT-IR spectrometer. Typically these FT-IRs use low-
power visible (red) lasers and momentary exposure to the beam is not dangerous,
but deliberate, direct viewing of the beam along its axis could damage your eyes.
DO NOT use a flammable gas to purge the MultiScope System Microscope. The
microscope contains a hot lamp, and fire or explosion may result. Use only clean,
dry oil-free nitrogen or air to purge the instrument.
DO read the more detailed information on safety in the following pages.
WARNING
Removal of covers and fixtures not specified in this manual may
result in hazardous radiation exposure.
Use of controls or adjustments or performance of procedures
other than those specified herein may result in hazardous
radiation exposure.

Warnings and Safety Information . 17
General Operating Conditions
The MultiScope System Microscope has been designed and tested in accordance
with PerkinElmer specifications and in accordance with the safety requirements of
the International Electrotechnical Commission (IEC). The MultiScope System
Microscope conforms to IEC 61010-1 (Safety requirements for electrical equipment
for measurement, control, and laboratory use) as it applies to IEC Class 1 (earthed)
appliances and therefore meets the requirements of EC Directive 2006/95/EC.
If the microscope is used in a manner not specified by the manufacturer, the
protection provided by the microscope may be impaired.
Only use the MultiScope System Microscope indoors under the following conditions:
Temperature 15 °C to 35 °C
Relative humidity 75% maximum (non-condensing)
If possible, avoid any adjustment, maintenance and repair of the opened, operating
instrument. If any adjustment, maintenance and repair of the opened instrument is
necessary, this must be done by a skilled person who is aware of the hazard
involved.
Whenever it is likely that the microscope is unsafe, make it inoperative. The
microscope may be unsafe if it:
•Shows visible damage;
•Fails to perform the intended measurement;
•Has been subjected to prolonged storage in unfavorable conditions;
•Has been subjected to severe transport stresses.

18 . MultiScope System Microscope User's Reference
Environmental Conditions
The microscope has been designed to be safe under the following conditions:
•Indoor use;
•Altitude up to 2000 m;
•Ambient temperatures of 5 °C to 40 °C;
•A maximum ambient relative humidity of 80% for temperatures up to 31 °C,
decreasing linearly to 50% relative humidity at 40 °C;
•Mains fluctuations not exceeding ±10% of the nominal voltage.

Warnings and Safety Information . 19
Electrical Safety
Connect the MultiScope System Microscope to a power supply line that includes a
switch or other adequate means of disconnection from the electricity supply.
Plug the microscope only into an electricity-supply socket that is provided with a
protective ground (earth) connection.
If fuses need replacing, use only those with the required current rating and of the
specified type. Do not use makeshift fuses and do not short-circuit fuse holders.
When replacing the fuse drawer on the mains inlet connector make sure that the
drawer is in the correct orientation for your mains supply. The drawer is also the
voltage selector.
When the MultiScope System Microscope is connected to its electricity supply,
terminals may be live. Removing covers other than those which can be removed by
hand is likely to expose live parts.
Capacitors inside the microscope may still be charged even if the microscope has
been disconnected from all voltage sources.
Disconnect the microscope from all voltage sources before it is opened for any
adjustment, replacement, maintenance or repair.
When replacing lamps, remember that the surface may be hot. The lamp and
surrounding area may exceed 30 °C above ambient.
WARNING
Any interruption of the protective ground (earth) conductor inside
or outside the microscope or disconnection of the protective earth
terminal can make the microscope dangerous.

20 . MultiScope System Microscope User's Reference
The MultiScope System Microscope has:
•An IEC Pollution Degree 2 classification - usually only non-conductive
atmospheric pollution of the equipment occurs; occasionally, however, a
temporary conductivity caused by condensation must be expected.
•An IEC Installation Category (Overvoltage Category) II classification - suitable
for connection to local level power supplies.
The instrument is designed to be safe under transient overvoltages typically present
on the MAINS supply.
NOTE: The normal level of transient overvoltages is impulse withstand
(Overvoltage) category II of IEC 60364-4-443.