PerkinElmer Spotlight 200 User manual

Spotlight 200
User’s Guide
MOLECULAR SPECTROSCOPY

Release History
Part Number Release Publication Date
L1050036 H October 2015
Any comments about the documentation for this product should be addressed to:
User Assistance
PerkinElmer, Inc.
710 Bridgeport Avenue
Shelton, Connecticut 06484-4794
U.S.A.
Or emailed to: [e-mail]
Notices
The information contained in this document is subject to change without notice.
Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes no
warranty of any kind with regard to this document, including, but not limited to, the
implied warranties of merchantability and fitness for a particular purpose.
PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in
connection with furnishing, performance or use of this material.
Copyright Information
This document contains proprietary information that is protected by copyright.
All rights are reserved. No part of this publication may be reproduced in any form whatsoever or
translated into any language without the prior, written permission of PerkinElmer, Inc.
Copyright © 2015 PerkinElmer, Inc.
Produced in the U.S.A.
Trademarks
Registered names, trademarks, etc. used in this document, even when not specifically marked as such,
are protected by law.
PerkinElmer is a registered trademark of PerkinElmer, Inc.
Fluorolube is a registered trademark of the Hooker Chemical Corporation
Nujol is a registered trademark of the Esso Standard Oil Company
Spotlight, Frontier, Spectrum Two and Spectrum are trademarks of PerkinElmer, Inc.

Contents
Introduction ............................................................................................... 7
About this Manual ............................................................................................. 8
Conventions Used ............................................................................................. 9
Notes, Cautions and Warnings..................................................................... 9
Definitions.................................................................................................12
Related Documents....................................................................................12
Requirements for using the Spotlight 200....................................................12
Connecting the PC to the Local Area Network..............................................12
Warnings and Safety Information ........................................................... 13
The Spotlight 200.............................................................................................14
Summary.........................................................................................................15
General Operating Conditions............................................................................16
Electrical Safety ...............................................................................................17
Location and Ventilation ...................................................................................18
Warning Labels ................................................................................................19
Microscope Safety Labels ...........................................................................19
Stage Controller Safety Labels ....................................................................20
Warning Signs on the Microscope ...............................................................21
Mechanical Safety ............................................................................................22
Lifting the Spotlight 200 ...................................................................................23
EMC Compliance ..............................................................................................24
System Requirements.......................................................................................25
Electrical Requirements..............................................................................25
Safety Specifications.........................................................................................27
Microscope................................................................................................27
Stage Controller ........................................................................................27
Overview of the Spotlight 200 .................................................................29
A Guided Tour of the Spotlight 200 ...................................................................30
Connections .....................................................................................................31
Operation ........................................................................................................33
The Optical System ..........................................................................................34
Visible Light Optics ....................................................................................34
Infrared Optics ..........................................................................................37
Spotlight System Requirements.........................................................................39
Hardware Requirements.............................................................................39
Software Requirements..............................................................................39
Getting Ready to Use the Spotlight 200 .................................................. 41
Before Using the Spotlight 200..........................................................................42
Cooling the MCT Detector .................................................................................43
Setting up the Spotlight 200 .............................................................................46
Focusing the Microscope ............................................................................46
Fitting an Attenuator to the Spectrometer ...................................................48
Setting Scan Parameters ............................................................................48
Preparing Samples ...................................................................................51
Preparing Samples ...........................................................................................52
Tools for Sample Preparation ............................................................................53
Tools Provided with the Spotlight 200 .........................................................53
Other Useful Tools.....................................................................................55
Specialized Accessories ..............................................................................55
Items to Have Available .............................................................................56
Common Window Materials...............................................................................57
Techniques for Preparing Samples for Transmittance Measurements ...................58
Flattening Solids ........................................................................................58
Slicing Samples from Solids ........................................................................59
Polymers...................................................................................................60
Particles ....................................................................................................62

4 . Spotlight 200 User’s Guide
Fibers....................................................................................................... 63
Fibrous Solids ........................................................................................... 63
Coatings on Substrates.............................................................................. 64
Liquids ..................................................................................................... 64
Techniques for Collecting Spectra ...........................................................65
Techniques for Collecting Spectra ..................................................................... 66
Using the Compression Cell ....................................................................... 66
Using a Hot/Cold Stage ............................................................................. 67
Collecting the Spectrum of a Thick Sample ................................................. 67
Collecting a Spectrum in an Inert Atmosphere ............................................ 70
Viewing a Sample with the Visible Polarizer ....................................................... 72
The Theory of Light Polarization................................................................. 72
Applications .............................................................................................. 73
Equipment................................................................................................ 74
Collecting IR Spectra Using the Infrared Polarizer Accessory .............................. 76
Using the Polarizer .................................................................................... 77
Collecting Spectra With the ATR Objective ........................................................ 78
ATR Objective Specification .............................................................................. 80
Fitting the ATR Crystal Holder to the Microscope ............................................... 81
Measuring the Maximum Energy in Reflectance Mode ........................................ 83
Adjusting the Height of the Crystal ................................................................... 84
Centering the Crystal................................................................................. 85
Checking the Infrared Alignment................................................................ 86
Removing the ATR Crystal Holder from the Microscope ...................................... 87
Fitting a Crystal Assembly to the ATR Objective................................................. 88
Manual ATR Objective ............................................................................... 88
Automated ATR Objective.......................................................................... 88
Cleaning the ATR Objective Crystal ................................................................... 90
Auto ATR Cleaning Procedure........................................................................... 91
Materials Needed ...................................................................................... 91
Cleaning Procedure ................................................................................... 91
Changing the Weighbridge Battery ................................................................... 96
Collecting Spectra............................................................................................ 98
Manual ATR Objective ............................................................................... 98
Automated ATR Objective........................................................................ 100
Analyzing Samples Using the ATR Imaging Accessory ...................................... 104
Correcting for the Effect of the ATR Crystal .............................................. 107
Reflectance FT-IR Microspectroscopy .............................................................. 109
Diffuse Reflectance ................................................................................. 109
Specular Reflectance ............................................................................... 110
Reflection-Absorption .............................................................................. 111
Maintenance...........................................................................................112
Maintenance.................................................................................................. 113
Inspecting the Microscope ....................................................................... 113
Protecting the Microscope........................................................................ 114
Cleaning ................................................................................................. 114
Cleaning the Cover.................................................................................. 114
Replacing the Microscope Fuse ....................................................................... 115
Testing the Stage Controller ........................................................................... 116
Replacing the Stage Controller Fuse................................................................ 117
Service.......................................................................................................... 118
Accessories ................................................................................................... 119
Sample Preparation Tools ........................................................................ 119
Electrical Connections .................................................................................... 120
Fitting the Plug ....................................................................................... 120
Connecting the Microscope to the Electrical Supply ................................... 121

Spotlight 200 User’s Guide . 5
Connecting the Microscope to the Spectrometer ........................................122
Connections to the Stage Controller ..........................................................123
Appendices.............................................................................................127
Appendix 1: Decontamination and Cleaning .....................................................128
Appendix 2: WEEE Instructions for PerkinElmer Products..................................129
Index ............................................................................................................130

6 . Spotlight 200 User’s Guide

Introduction

8 . Spotlight 200 User’s Guide
About this Manual
This introduction gives you information about this manual, to enable you to use it effectively
when learning to operate the Spotlight 200.
Before you start using your Spotlight 200 we recommend that you:
•Read the
Warnings and Safety Information starting on page 13;
•Read the rest of this
Introduction and become aware of conventions and requirements;
•Read
Overview of the Spotlight 200 starting on page 29;
•Read Getting Ready to Use the Spotlight 200 starting on page 41.
Four chapters in this User’s Guide contain information on using and maintaining your
Spotlight 200:
•
Getting Ready to Use the Spotlight 200 gives you information on how to set up your
Spotlight 200 at the beginning of the day's work, to make sure that it is working
properly.
•
Preparing Samples describes techniques for preparing many types of microscopic
samples. It includes descriptions of the sample preparation tools provided with, or
available for, the Spotlight 200.
•
Techniques for Collecting Spectra describes how you can use accessories and different
techniques to collect spectra.
•
Maintenance contains maintenance information. It gives you information on how to care
for the Spotlight 200 and about the performance checks that we recommend that you
perform routinely.

Introduction . 9
Conventions Used
Normal text is used to provide information and instructions. Italic text is used to provide
commentary. Bold text refers to text that is displayed on the screen.
UPPERCASE text, for example ENTER or ALT, refers to keys on the PC keyboard. “+” is
used to show that you have to press two keys at the same time, for example, ALT+F.
All eight digit numbers are PerkinElmer part numbers unless stated otherwise.
“Spectrometer” refers to the Frontier IR System, Spectrum 100 Series, Spectrum 400 Series
or Spectrum One spectrometer supplied with your Spotlight 200.
Notes, Cautions and Warnings
Three terms, in the following standard formats, are also used to highlight special
circumstances and warnings.
NOTE: A note indicates additional, significant information that is provided with some
procedures.

10 . Spotlight 200 User’s Guide
CAUTION
We use the term CAUTION to inform you about situations that could result
in serious damage to the instrument
or other equipment. Details about
these circumstances are in a box like this one.
Caution (Achtung)
Bedeutet, daß die genannte Anleitung genau befolgt werden muß, um einen
Geräteschaden zu vermeiden.
Caution (Bemærk)
Dette betyder, at den nævnte vejledning skal overholdes nøje for at undgå
en beskadigelse af apparatet.
Caution (Advertencia)
Utilizamos el término CAUTION (ADVERTENCIA) para advertir sobre
situaciones que pueden provocar averías graves en este equipo o en otros.
En los recuadros como éste se proporciona información sobre este tipo de
circunstancias.
Caution (Attention)
Nous utilisons le terme CAUTION (ATTENTION) pour signaler les
situations susceptibles de provoquer de graves détériorations de
l'instrument ou d'autre matériel. Les détails sur ces circonstances figurent
dans un encadré semblable à celui-ci.
Caution (Attenzione)
Con il termine CAUTION (ATTENZIONE) vengono segnalate situazioni
che potrebbero arrecare gravi danni allo strumento o ad altra
apparecchiatura. Troverete informazioni su tali circostanze in un riquadro
come questo.
Caution (Opgelet)
Betekent dat de genoemde handleiding nauwkeurig moet worden opgevolgd,
om beschadiging van het instrument te voorkomen.
Caution (Atenção)
Significa que a instrução referida tem de ser respeitada para evitar a
danificação do aparelho.

Introduction . 11
WARNING
We use the term WARNING to inform you about situations that could
result in personal injury to yourself or other persons. Details about these
circumstances are in a box like this one.
Warning (Warnung)
Bedeutet, daß es bei Nichtbeachten der genannten Anweisung zu einer
Verletzung des Benutzers kommen kann.
Warning (Advarsel)
Betyder, at brugeren kan blive kvæstet, hvis anvisningen ikke overholdes.
Warning (Peligro)
Utilizamos el término WARNING (PELIGRO) para informarle sobre
situaciones que pueden provocar daños personales a usted o a otras
personas. En los recuadros como éste se proporciona información sobre
este tipo de circunstancias.
Warning (Danger)
Nous utilisons la formule WARNING (DANGER) pour avertir des
situations pouvant occasionner des dommages corporels à l'utilisateur ou
à d'autres personnes. Les détails sur ces circonstances sont données dans
un encadré semblable à celui-ci.
Warning (Pericolo)
Con il termine WARNING (PERICOLO) vengono segnalate situazioni
che potrebbero provocare incidenti alle persone. Troverete informazioni
su tali circostanze in un riquadro come questo.
Warning (Waarschuwing)
Betekent dat, wanneer de genoemde aanwijzing niet in acht wordt
genomen, dit kan leiden tot verwondingen van de gebruiker.
Warning (Aviso)
Significa que a não observância da instrução referida poderá causar um
ferimento ao usuário.

12 . Spotlight 200 User’s Guide
Definitions
OPERATOR: Person operating the equipment for its intended purpose.
RESPONSIBLE BODY: Individual or group responsible for the use and maintenance of the
equipment and for ensuring that the OPERATORS are adequately trained.
Related Documents
Manuals for Spectrometer
Information on using your spectrometer can be found in the manuals that were supplied with
it. The
IR & Raman Manuals CD (L1050002) includes instrument manuals for the Frontier IR
Systems, Spectrum 400 Series and Spectrum 100 Series spectrometers. The
Spectrum Two
Manuals CD
(L1050242) contains the instrument manual for the Spectrum Two spectrometer.
Spectrum Software
Spectrum software has on-screen Help, which you can access by choosing the Contents
command from the Help menu, by pressing the F1 key, or by clicking the Help button in a
dialog. The Help information assumes that you are familiar with the hardware components of
the Spotlight 200 microscope contained in this guide.
Requirements for using the Spotlight 200
We assume that the Spotlight 200 has been properly set up and aligned. This installation
must be performed by a PerkinElmer Service Engineer.
Connecting the PC to the Local Area Network
The PC provided is connected point-to-point to the Spotlight 200. If you wish to connect the
PC to your local area network to enable transfer of data, we recommend that you contact
your local PerkinElmer Service Engineer.

Warnings and Safety
Information

14 . Spotlight 200 User’s Guide
The Spotlight 200
The Spotlight 200 consists of a microscope, spectrometer, PC, stage controller and joystick.
Figure 1 Spotlight 200 – microscope and Frontier IR System
Figure 2 Spotlight 200 – microscope and Spectrum Two System

Warnings and Safety Information . 15
Summary
The PerkinElmer Spotlight 200 has been designed to comply with a wide variety of
international standards governing the safety of laboratory equipment. In routine use, the
Spotlight 200 poses virtually no risk to you. If you take some simple, common-sense
precautions, you can make sure that you maintain the continued safe operation of the
Spotlight 200.
•DO make sure that all parts of the Spotlight 200 are properly connected to the electrical
supply; in particular, make sure that the ground (earth) wires are securely connected.
•DO disconnect the electrical power supply before opening the main cover of the
microscope and spectrometer.
•DO keep the microscope dry. Avoid spilling liquid into the microscope and spectrometer.
Clean all external spills immediately. If anything that is spilled enters the main body of
the microscope or spectrometer, switch off the power and call a PerkinElmer Service
Engineer.
•If your Spotlight 200 is fitted with an MCT (mercury cadmium telluride) detector,
DO wear safety glasses and protective gloves when you are filling the detector dewar in
the microscope with liquid nitrogen. Slowly pour the liquid nitrogen into the dewar.
Stand back from the detector during filling because liquid nitrogen may be expelled
from the dewar flask. Use only liquid nitrogen.
•DO NOT use a flammable gas to purge the spectrometer or microscope. The
spectrometer contains a hot lamp, and fire or explosion may result. Use only clean, dry,
oil-free nitrogen or air to purge the instrument.
•If your Spotlight 200 is fitted with an automated ATR objective, DO NOT look directly at
either the stage lighting LEDs or the window at the back of the weighbridge, particularly
if you are using an optical magnifier that could focus the visible or infrared beams from
these sources.
•DO read the more detailed information on safety in the following pages.

16 . Spotlight 200 User’s Guide
General Operating Conditions
For information about the spectrometer, refer to the User’s Guide supplied with the
instrument. The
IR & Raman Manuals CD (L1050002) includes instrument manuals for the
Frontier IR Systems, Spectrum 400 Series and Spectrum 100 Series spectrometers.
The microscope and stage controller have been designed and tested in accordance with
PerkinElmer specifications and in accordance with the safety requirements of the
International Electrotechnical Commission (IEC). The microscope and stage controller
conform to IEC publication 61010−1 (“Safety requirements for electrical equipment for
measurement, control, and laboratory use”) as it applies to IEC Class 1 (earthed) appliances
and therefore meets the requirements of EC low voltage directive 2006/95/EC.
Only use the microscope and stage controller indoors and under the following conditions:
Temperature 15 °C to 35 °C
Relative humidity 80% maximum (non-condensing)
If possible, avoid any adjustment, maintenance and repair of the opened, operating
instrument. If any adjustment, maintenance and repair of the opened instrument is
necessary, this must be done by a skilled person who is aware of the hazard involved.
Whenever it is likely that the microscope and stage controller are unsafe, make them
inoperative. The microscope and stage controller may be unsafe if they:
•Show visible damage;
•Fail to perform the intended measurement;
•Have been subjected to prolonged storage in unfavorable conditions;
•Have been subjected to severe transport stresses.
The microscope and stage controller have been designed to be safe under the following
conditions:
•Indoor use;
•Altitude up to 2000 m;
•Ambient temperatures of 5 °C to 40 °C;
•A maximum ambient relative humidity of 80% for temperatures up to 31 °C decreasing
linearly to 50% relative humidity at 40 °C;
•Mains fluctuations not exceeding ±10% of the nominal voltage. For example,
230 V ± 10%.
WARNING
If the equipment is used in a manner not specified herein the protection
provided by the equipment may be impaired.

Warnings and Safety Information . 17
Electrical Safety
Connect the microscope and stage controller to a power supply line that includes a switch or
other adequate means of disconnection from the electricity supply.
Only plug the microscope and stage controller into electricity-supply sockets that are
provided with a protective ground (earth) connection. The stage control box and the
microscope must be earthed.
If fuses need replacing, use only those with the required current rating and of the specified
type. Do not use makeshift fuses and do not short-circuit fuse holders.
When the microscope and stage controller are connected to its electricity supply, terminals
may be live. Removing covers other than those which can be removed by hand is likely to
expose live parts.
NOTE: There are no user-serviceable parts in the microscope or the stage controller.
Capacitors inside the microscope and stage controller may still be charged even if the
microscope or stage controller has been disconnected from all voltage sources.
Disconnect the microscope and stage controller from all voltage sources before they are
opened for any adjustment, replacement, maintenance or repair. Any adjustment,
replacement, maintenance or repair must be performed by a PerkinElmer Service Engineer.
The microscope and stage controller must only be connected to equipment meeting the
requirements of IEC 61010-1 (Safety requirements for electrical equipment for measurement,
control and laboratory use – general requirements) or IEC 60950 (Safety of information
technology equipment).
WARNING
Any interruption of the protective ground (earth) conductor inside or
outside the microscope or stage controller or disconnection of the
protective ground (earth) terminal can make the microscope or stage
controller dangerous.

18 . Spotlight 200 User’s Guide
Location and Ventilation
The Spotlight 200 is installed by a PerkinElmer Service Engineer, who will be able to advise
on the positioning of the system. To allow for adequate cooling, the system should not be
sited near to room heating equipment, for example, central-heating radiators. There should
be a minimum gap of at least 15 cm (6 inches) from the top and side surfaces of the
microscope and stage control box to permit adequate cooling.
WARNING
Make sure that the switches at the electrical supply inlet at the rear of
the microscope and stage controller are not obstructed.

Warnings and Safety Information . 19
Warning Labels
When this label is attached to an instrument it means “Caution, risk of
danger”. Refer to the manual to find out the nature of the potential
hazard and any actions which have to be taken.
Microscope Safety Labels
The following safety labels are fixed to the microscope.
Figure 3 Rear of Microscope
Power
100–230V ~50/60Hz
75 VA

20 . Spotlight 200 User’s Guide
Figure 4 Inside Dewar lid – MCT detector versions only
NOTE: See Cooling the MCT Detector on page 43 for information on how to fill the Dewar.
Stage Controller Safety Labels
Figure 5 Rear of Stage Controller
MAINS INPUT
100–240 V /AC
60 VA
50/60 Hz
FUSE 1.6 A T
Warning
Disconnect supply
cord before opening