PHYWE MIC-200 Series User manual

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www.phywe.com, © All rights reserved MIC-200 Series / 1720
Operating instructions
Student microscopes MIC-200 Series
MIC-222, MIC-231
PHYWE Systeme GmbH & Co. KG
Robert-Bosch-Breite 10
D-37079 Göttingen
Telefon [phone]
Fax [phone]
E-mail [email protected]
Internet www.phywe.com
Fig. 1:MIC-222 (left) und MIC-231 (right)
The unit complies
with the correspond-
ing EC guidelines.
INHALTSVERZEICHNIS
1INTRODUCTION
2CONSTRUCTION OF THE MICROSCOPE
3PREPARING THE MICROSCOPE
4FUNCTIONS OF THE MICROSCOPE
5WORKING WITH THE MICROSCOPE
6MAINTENANCE AND CLEANING
7TECHNICAL DATA
8WASTE DISPOSAL
1 INTRODUCTION
By purchasing a microscope from the MIC-200 series, you
have chosen a quality microscope. The MIC-200 series mi-
croscopes are designed for use in schools and university
student labs as well as and laboratories.
This instruction manual describes how to set up the micro-
scope, how to use, clean and maintain it.
The MIC-200 microscopes differ as follows:
Model
Binocular / trinocular
MIC-222
Binocular
MIC-231
Trinocular
2 CONSTRUCTION OF THE MICROSCOPE
After completing the step "Preparing the microscope", first
familiarize yourself with the mechanical, but also the optical
and electrical components of your microscope. Operate each
component carefully by hand and get an impression of the
functionality of each adjustment option.

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The names of the components are listed here and are
marked in the Fig. 1 & 2.
A1) Eyepiece
A2) Revolving nosepiece for 5 objectives
A3) and A4) Mechanical stage with specimen holder
A5) Abbe condenser
A6) Iris diaphragm
A7) Condenser holder
A8) Filter holder
A9) Polarizer (optional)
A10) Binocular head
A11) Head lock screw
A12) Main body
A13) Phase contrast slide / darkfield slide slot
A14) and A15) Coaxial drive for X and Y movement of the
sample holder
A16) Light intensity adjustment knob
B1) 1X C-mount (MIC-231)
B2) Trinocular observing tube
B3) Lifting handle
B4) On/off switch
B5) Fuse socket
B6) Power socket
B7) Diopter adjustment ring on left eyepiece
B8) Objectives (4x, 10x, 40x, 100x)
B9) Focusing knob of condenser
B10) Adjustment handwheel of coarse focusing mechanism
B11) and B12) Coaxial coarse and fine focusing mechanism
3 PREPARING THE MICROSCOPE
Unpack the microscope and place it on a table. Take out the
main body and observing tube, connect them together, and
then use the attached Allen wrench to tight the observing
tube lock screw.
The objectives 4x, 10x, 40x and 100x objectives are pre-
assembled with this microscope.
Plug in the power plug and switch on the microscope. Now sit
comfortably in front of the microscope to use it.
4 FUNCTIONS OF THE MICROSCOPE
The stand consists of observing head, base and table. You
can grip the microscope by the lifting handle during transport.
Binocular or trinocular head
The head with the tubes can be rotated through 360° and is
equipped with two WF10x wide-field eyepieces. These are
marked with a spectacle symbol, because they are eyepieces
for spectacle wearers. This enables spectacle wearers with
glasses to perform microscopy without removing the specta-
cles.
Revolving nosepiece
The nosepiece is equipped with 4 achromatic objectives as
follows:
4x NA 0.13
10x NA 0.30
S40x NA 0.70
S100x NA 1.25 (oil)
The objectives 40x, and 100x are equipped with springs (S)
to prevent damage to the front lens and the microscope slide
and cover glass).
The numerical aperture (N.A.) of the objective indicates the
resolving power of the objective.
Fig. 2 MIC-231
Fig. 1 MIC-222

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The total magnification can be easily calculated by multiply-
ing the magnification of the eyepiece by that of the respective
objective, as shown in the table:
Ocular
Objective
Magnification
10x
4x
40x
10x
10x
100x
10x
40x
400x
10x
100x
1000x
In MIC-200 series microscopes, the objectives are already
attached to the nosepiece.
Check that all the objectives are firmly attached. To do this,
grasp the knurled rings of the objectives with your fingers and
turn them to the right. Do not use a tool to tighten them. The
entire nosepiece with the 4 objectives can be turned by hand.
When you rotate the nosepiece, you will notice that the objec-
tives click into place. They do so in the correct position rela-
tive to the optical axis of the microscope.
Object table with mechanical stage
The microscope slide is placed in the specimen holder of the
mechanical stage and can be moved in X and Y direction.
The stage has a working area of 141 x 131 mm, the travel
range is 78 x 53 mm.
Coarse and fine adjustment
The focusing mechanisms for coarse and fine adjustment are
located on one axis (coaxial). On the fine adjustment knobs
there is a graduation. This can be used to measure the depth
of a specimen.
When the stage automatically slides down after using the mi-
croscope for some time, turn the adjustment ring on the in-
side of the coarse and fine adjustment knobs slightly in the
direction of the arrow. The coarse adjustment knob is tight-
ened to prevent the stage from sliding down.
Abbe condenser with iris diaphragm
Below the object stage is an Abbe condenser N.A. 1.25. The
condenser can be adjusted in height by turning. This allows
light to be focused on the specimen for contrast enhance-
ment. The condenser is already pre-centered.
The iris diaphragm with filter holder is located under the con-
denser. The light intensity can be adjusted by changing the
movable opening.
Illumination
The power-saving illumination is the reason why the micro-
scopes of the MIC-222 model do not need to be connected to
the mains all the time for operation, but can also be operated
independently of the mains, as they contain a rechargeable
Ni-MH battery. The illumination is a modern LED lamp with
the following specifications:
LED: 3W, brightness adjustable
Power supply: 100V-230V
C-mount adapter for the trinocular version MIC-231
A C-mount adapter is part of the scope of delivery of the MIC-
231 so that you can use each microscope camera which has
a C-mount thread.
5 WORKING WITH THE MICROSCOPE
Please follow the following instructions to achieve the best
results:
Adjustment of interpupillary distance and diopter dis-
tance
1. Adjusting the interpupillary distance
While looking through the eyepieces, adjust them until the left
and right fields of view coincide completely.
2. Adjusting the diopter
a) While looking through the right eyepiece, rotate the
coarse/fine focusing adjustment knobs to bring the specimen
into focus.
b) Look through the left eyepiece and rotate only the diopter
adjustment ring on the left eyepiece sleeve to bring the spec-
imen into focus.
Adjusting the lighting
For optimal contrast and resolution, please follow these
steps:
Place a micro slide on the stage and focus with the 4x
lens. The iris diaphragm is open.
Turn the condenser to the highest position.
Close the iris diaphragm until it is just visible at the edge
of the image field.
The microscope is now optimized for use with the 4x ob-
jective. Repeat this procedure with each additional magni-
fication to achieve the best balance between contrast and
resolution.
Attention: the maximum light intensity of the microscope
can damage your eyes if you use it with the 4x and 10x
objectives!
If you swap micro slides, start again with the 4x objective.
It is recommended to use slides of 1.0 to 1.2 mm thickness in
combination with cover glasses of 0.13 mm or 0.17 mm
thickness.
Battery
The MIC-222 microscope contains a 3.6V Ni-MH battery
pack.
A charging indicator is located on the side of the microscope.
It is red when connected to the power supply and green when
the battery is full. If the red indicator flickers, the battery is not
properly connected to the circuit board.
The microscope's illumination can be powered by a battery
pack for 4 to 8 hours without an external power supply.
Using the S100x oil immersion objective
Please follow the following recommendations for using S100x
N.A. 1.25 oil immersion lens:

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Focus the image with the S40x lens.
Rotate the revolving nosepiece until the S100x objective
almost (not quite) clicks into place.
Drop a small drop of immersion oil (not part of the scope
of delivery, please order separately) on the center of the
specimen.
Then let the S100x objective snap into place.
The front lens is now in contact with the immersion oil.
View the specimen through the eyepiece and focus the
image with the fine adjustment knob.
The distance between objective lens and specimen is on-
ly 0.24mm!
If small bubbles are visible, turn the S100x objective a
few times to the left and right so that the front lens of the
objective moves in the oil. This will make the bubbles
disappear.
Then turn the stage with the fine adjustment knob down-
wards until the front lens does not touch the oil anymore.
Always clean the front lens of the S100x lens with a piece
of lens paper moistened with a drop of xylene or alcohol.
Now also clean the micro slide.
The S100x objective can also be used without immersion oil,
i.e. dry. However, please note that in this case the resolution
is much lower. Water may provide somewhat better resolu-
tion than using it dry.
Caution!
Never drop the xylene or alcohol directly onto the ob-
jective lens. This could cause xylene or alcohol to en-
ter the lens and dissolve the lens fixation!
Avoid oil contact with the other lenses!
6 MAINTENANCE AND CLEANING
Always cover the microscope with a dust cover after use. Al-
ways leave the eyepieces and objective lenses mounted so
that no dust can enter the inside parts of the microscope.
Cleaning the optical components
If the eyepiece lenses or the front lenses of the objectives are
dirty, you can clean them by wiping a piece of lens paper
over the lens surface in circular motions. If this does not help,
use a drop of xylene or alcohol on the lens paper. Never put
xylene or alcohol directly on the lens!
If dirt is clearly visible in the field of view, it is on the lowest
lens of the eyepiece. Remove the eyepiece from the tube and
clean the outside of the lens.
If dust is still visible, check whether the dust is in the eye-
piece by rotating it. If so, gently remove the bottom lens from
the eyepiece and clean it.
It is not necessary - and not recommended - to clean the lens
surfaces inside the objectives. Sometimes the dust can be
removed by high-pressure treatment with air. Otherwise,
there will be no dust inside the objectives until the objectives
are removed from the revolving nosepiece.
The most important step to a long lasting clean microscope
optics is to use the dust cover when you are not using your
microscope!
Caution! Cleaning cloths containing plastic fibers can
damage the coating of the lenses!
Maintenance of the stand and mechanical components
Simply remove dust with a brush. If the stand is very dirty, the
surface can be treated with a careful cleaning agent.
All moving parts such as the adjustment of the mechanical or
the coarse and fine focusing mechanism contain ball bear-
ings which are not sensitive to dust. The bearings can be
relubricated with sewing machine oil if necessary.
Change the lamp and the fuse
Switch off the power and pull the mains plug.
Now loosen the lower cover screw of the chassis and re-
move the cover to replace the bulb and fuse according to
the requirements of the different models.
After replacing the bulb and fuse, the cover should be re-
placed and the screws tightened.
7 TECHNICAL DATA
Infinity optical system
Siedentopf viewing head, tubes: 30° inclined (binocu-
lar/trinocular), 360° rotatable
WF10x/18mm eyepieces for spectacle wearers
Eyepieces of the monocular models secured against re-
moval
Quintuple nosepiece with click-stop
Achromatic objectives 4x NA 0.13, 10x NA 0.30, S40x NA
0.70, and S100x NA 1.25 (oil)
Condenser: Abbe N.A. 1.25 with iris diaphragm
Models with mechanical stage: area 141 x 131 mm, travel
range 78 x 53 mm
Coaxial coarse and fine focusing mechanism with gradua-
tions on the fine focusing mechanism
Lighting: 3 W LED (colour temperature 5000 K), adjusta-
ble
Integrated power supply unit 100...240 V, 50/60 Hz, 3W
Fuse: F3.15A/250V
Includes dust cover
Dimensions (H x W x L): 39 cm x 23 cm x 28 cm
Weight: 8.5 kg (MIC-222); 8.6 kg (MIC-231)
8 WASTE DISPOSAL
The packaging consists predominately of environmentally
compatible materials because they can be recycled and
should be passed on for disposal by the local recycling ser-
vice.
Should you no longer require this product,
do not dispose of it with the household re-
fuse.
Please return it to the address below for
proper waste disposal.
PHYWE Systeme GmbH & Co. KG
Service Department (Customer Service)
Robert-Bosch-Breite 10
37079 Göttingen / Germany
Phone [phone]
Fax [phone]
Mail [email protected], [email protected]