PHYWE MIC-100 Series User manual

1
www.phywe.com, © All rights reserved MIC-100 Series / 1820
Operating instructions
Student microscopes MIC-100 Series
MIC-110A, MIC-111A, MIC-
116A, MIC-119A, MIC-121A,
MIC-126A, MIC-129A
PHYWE Systeme GmbH & Co. KG
Robert-Bosch-Breite 10
D-37079 Göttingen
Telefon [phone]
Fax [phone]
E-mail [email protected]
Internet www.phywe.com
Fig. 1: MIC-110A (left) und MIC-126A (right)
The unit complies
with the correspond-
ing EC guidelines.
INHALTSVERZEICHNIS
1INTRODUCTION
2CONSTRUCTION OF THE MICROSCOPE
3PREPARING THE MICROSCOPE
4FUNCTIONS OF THE MICROSCOPE
5WORKING WITH THE MICROSCOPE
6MAINTENANCE AND CLEANING
7TECHNICAL DATA
8WASTE DISPOSAL
1 INTRODUCTION
By purchasing a microscope from the MIC-100 series, you
have chosen a quality microscope. The MIC-100 series micro-
scopes are designed for use in schools and laboratories.
This instruction manual describes how to set up the micro-
scope, how to use, clean and maintain it.
The MIC-100 microscopes differ as follows:
Model
Mono- /
binocular
Table
Magnifica-
tion
MIC-110A
Monocular
Clamps
400x
MIC-111A
Monocular
Mechanical
stage
400x
MIC-116A
Monocular
Mechanical
stage
600x
MIC-119A
Monocular
Mechanical
stage
1000x
MIC-121A
Binocular
Mechanical
stage
400x
MIC-126A
Binocular
Mechanical
stage
600x
MIC-129A
Binocular
Mechanical
stage
1000x
2 CONSTRUCTION OF THE MICROSCOPE
After completing the step "Preparing the microscope", first fa-
miliarize yourself with the mechanical, but also the optical and
electrical components of your microscope. Operate each com-
ponent carefully by hand (no tools are necessary) and get an
impression of the functionality of each adjustment option.

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The names of the components are listed here and are marked
in the Fig. 2. Shown is the binocular version with mechanical
stage:
A1) Right tube with eyepiece (10x)
A2) Left tube with eyepiece (10x)
A3) Diopter adjustment ring on left eyepiece
A4) Adjusting the interpupillary distance
A5) Microscope head, rotatable
A6) Revolving nosepiece for 4 objectives
A7) Objectives (4x, 10x, 40x, possibly 60x or 100x depending
on the model)
A8 with A9) Work table (shown here: mechanical stage with
specimen holder, alternatively object stage with clamps)
A10 and A11) Coaxial coarse and fine focussing mechanism
A12 and A13) Only for models with mechanical stage: coaxial
handwheels for X and Y movement of the sample holder
A14) Collective lens of the LED illumination
A15) Light intensity adjustment knob
Below the object stage: Condenser with iris diaphragm and fil-
ter holder (not visible)
On the back: On/Off switch (not visible)
Fig. 2 Set up of the microscope
Fig. 3 Object stage with clamps
3 PREPARING THE MICROSCOPE
Unpack the microscope and place it on a table. The 4x, 10x
and 40x objectives are pre-assembled with this microscope.
Otherwise, proceed as described for the 60x and 100x objec-
tives.
If you have a version with 600x or 1000x magnification, 60x
and 100x objectives are supplied with the microscope outside
the polystyrene package and must be locked in place:
Attach the lenses by removing the protective cap from the free
space on the nosepiece and simply screw in the additional
lens. However, do not tighten it with a tool.
Plug in the power plug and switch on the microscope. Now sit
comfortably in front of the microscope to use it.
4 FUNCTIONS OF THE MICROSCOPE
The stand consists of tube arm (tube carrier), base and table.
You can grip the microscope by the tube arm during transport.
Monocular or binocular tube(s)
The head with the tube(s) can be rotated through 360° and is
equipped with two WF10x wide-field eyepieces. These are
marked with a spectacle symbol, because they are eyepieces
for spectacle wearers. This enables spectacle wearers with
glasses to perform microscopy without removing the specta-
cles.
The eyepieces are secured against removal, but can be re-
moved and replaced with a tool, e.g. for cleaning.
Revolving Nosepiece
The nosepiece is equipped with 3 or 4 achromatic objectives
as follows:
The microscopes with 400x magnification: 4x NA 0.10, 10x
NA 0.25, S40x NA 0.65
The microscopes with 600x magnification: 4x NA 0.10, 10x
NA 0.25, S40x NA 0.65, S60x NA 0.85
The microscopes with 1000x magnification: 4x NA 0.10,
10x NA 0.25, S40x NA 0.65, S100x NA 1.25
The objectives 40x, 60x and 100x are equipped with springs
to prevent damage to the front lens and the microscope slide
and cover glass).
The numerical aperture (N.A.)of the objective indicates the re-
solving power of the objective.
The total magnification can be easily calculated by multiplying
the magnification of the eyepiece by that of the respective ob-
jective, as shown in the table:
Ocular
Objective
Magnification
10x
4x
40x
10x
10x
100x
10x
40x
400x
10x
60x
600x
10x
100x
1000x

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In microscopes with 400x magnification, the objectives are al-
ready attached to the nosepiece.
For the microscopes with 600x and 1000x magnification, the
60x and 100x objectives are not yet attached to the revolving
nosepiece, but are part of the scope of delivery.
Check that all the objectives are firmly attached. To do this,
grasp the knurled rings of the objectives with your fingers and
turn them to the right. Do not use a tool to tighten them. The
entire nosepiece with the 3 or 4 objectives can be turned by
hand. When you rotate the nosepiece, you will notice that the
objectives click into place. They do so in the correct position
relative to the optical axis of the microscope.
Object table with preparation clamps or mechanical stage
Object table/stage with preparation clamps: The micro slide is
locked in place with the two clamps. It is then carefully posi-
tioned with the fingers.
Mechanical stage: The microscope slide is placed in the spec-
imen holder of the mechanical stage and can be moved in X
and Y direction. The stage has a working area of 140 x 130
mm, the travel range is 78 x 30 mm.
Coarse and fine adjustment
The focussing mechanisms for coarse and fine adjustment are
located on one axis (coaxial). On the fine adjustment knobs
there is a graduation. This can be used to measure the depth
of a specimen.
When the stage automatically slides down after using the mi-
croscope for some time, turn the adjustment ring on the inside
of the coarse and fine adjustment knobs slightly in the direction
of the arrow. The coarse adjustment knob is tightened to pre-
vent the stage from sliding down.
Abbe condenser with iris diaphragm
Below the object stage is an Abbe condenser N.A. 1.25. The
condenser can be adjusted in height by turning. This allows
light to be focused on the specimen for contrast enhancement.
The condenser is already pre-centered.
The iris diaphragm with filter holder is located under the con-
denser. The light intensity can be adjusted by changing the
movable opening.
Lighting
The power-saving illumination is the reason why the micro-
scopes of the MIC-100 series do not need to be connected to
the mains all the time for operation, but can also be operated
independently of the mains, as they contain a rechargeable
NiMH battery. The illumination is a modern LED lamp with the
following specifications:
LED: 3W, brightness adjustable
Power supply: 100V-230V
5 WORKING WITH THE MICROSCOPE
Please follow the following instructions to achieve the best re-
sults:
Adjustment of interpupillary distance and diopter dis-
tance for a binocular model
First adjust the coarse and fine adjustment knobs so that you
get a sharp microscope image with the right eyepiece (A1),
and then adjust the diopter adjustment ring (A3) on the left
eyepiece tube (A2) to get a sharp image with the left eyepiece
as well. Finally, adjust the interpupillary distance (A4) so that
it corresponds to the viewer's interpupillary distance. Some-
times the microscope head (A5) should be rotated to obtain a
comfortable observation position instead of moving the micro-
scope body.
This procedure must be carried out anew for each user.
Adjusting the lighting
For optimal contrast and resolution, please follow these steps:
Place a micro slide on the stage and focus with the 4x lens.
The iris diaphragm is open.
Turn the condenser to the highest position.
Close the iris diaphragm until it is just visible at the edge of
the image field.
The microscope is now optimized for use with the 4x ob-
jective. Repeat this procedure with each additional magni-
fication to achieve the best balance between contrast and
resolution.
Attention: the maximum light intensity of the microscope
can damage your eyes if you use it with the 4x and 10x
objectives!
If you swap microslides, start again with the 4x objective.
It is recommended to use slides of 1.0 to 1.2 mm thickness in
combination with cover glasses of 0.13 mm or 0.17 mm thick-
ness.
Battery
The microscope contains a 3.6V NiMH battery.
A charging indicator is located on the side of the microscope.
It is red when connected to the power supply and green when
the battery is full. If the red indicator flickers, the microscope is
not properly connected to the power supply.
The microscope's illumination can be powered by a battery
pack for 4 to 8 hours without an external power supply.
Using the S100x oil immersion objective
The MIC-119A and MIC-129A are equipped with an S100x
N.A. 1.25 oil immersion lens. Please follow the following
recommendations for using this lens:
Focus the image with the S40x lens.
Rotate the revolving nosepiece until the S100x objective
almost (not quite) clicks into place.
Drop a small drop of immersion oil (not part of the scope of
delivery, please order separately) on the center of the
specimen.
Then let the S100x objective snap into place.
The front lens is now in contact with the immersion oil.
View the specimen through the eyepiece and focus the im-
age with the fine adjustment knob.
The distance between objective lens and specimen is only
0.14mm!
If small bubbles are visible, turn the S100x objective a few
times to the left and right so that the front lens of the objec-
tive moves in the oil. This will make the bubbles disappear.
Then turn the stage with the fine adjustment knob down-
wards until the front lens does not touch the oil anymore.
Always clean the front lens of the S100x lens with a piece
of lens paper moistened with a drop of xylene or alcohol.
Now also clean the microslide.


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The S100x objective can also be used without immersion oil,
i.e. dry. However, please note that in this case the resolution
is much lower. Water may provide somewhat better resolution
than using it dry.
Caution!
Never drop the xylene or alcohol directly onto the ob-
jective lens.This could cause xylene or alcohol to enter
the lens and dissolve the lens fixation!
Avoid oil contact with the other lenses!
6 MAINTENANCE AND CLEANING
Always cover the microscope with a dust cover after use. Al-
ways leave the eyepieces and objective lenses mounted so
that no dust can enter the inside parts of the microscope.
Cleaning the optical components
If the eyepiece lenses or the front lenses of the objectives are
dirty, you can clean them by wiping a piece of lens paper over
the lens surface in circular motions. If this does not help, use
a drop of xylene or alcohol on thelens paper. Neverput xylene
or alcohol directly on the lens!
If dirt is clearly visible in the field of view, it is on the lowest
lens of the eyepiece. Remove the eyepiece from the tube and
clean the outside of the lens.
If dust is still visible, check whether the dust is in the eyepiece
by rotating it. If so, gently remove the bottom lens from the
eyepiece and clean it.
It is not necessary - and not recommended - to clean the lens
surfaces inside the objectives. Sometimes the dust can be re-
moved by high-pressure treatment with air. Otherwise, there
will be no dust inside the objectives until the objectives are re-
moved from the revolving nosepiece.
The most important step to a long lasting clean microscope
optics is to use the dust cover when you are not using your
microscope!
Caution! Cleaning cloths containing plastic fibres can
damage the coating of the lenses!
Maintenance of the stand and mechanical components
Simply remove dust with a brush. If the stand is very dirty, the
surface can be treated with a careful cleaning agent.
All moving parts such as the adjustment of the mechanical or
the coarse and fine focussing mechanism contain ball bear-
ings which are not sensitive to dust. The bearings can be relu-
bricated with sewing machine oil if necessary.
Change the lamp and the fuse
Switch off the power and pull the mains plug.
Now loosen the lower cover screw of the chassis and re-
move the cover to replace the bulb and fuse according to
the requirements of the different models.
After replacing the bulb and fuse, the cover should be re-
placed and the screws tightened.
7 TECHNICAL DATA
Tube: 30° viewing angle (monocular/binocular)
WF10x/18mm eyepieces for spectacle wearers
Eyepieces of the monocular models secured against re-
moval
4-position nosepiece with click-stop
Achromatic objectives 4x NA 0.10, 10x NA 0.25, S40x NA
0.65, S60x NA 0.85 and S100x (oil) NA 1.25 (depending
on model)
Condenser: Abbe N.A. 1.25 with iris diaphragm
Models with mechanical stage: area 140 x 130 mm, travel
range 78 x 30 mm
Coaxial coarse and fine focussing mechanism with gradu-
ations on the fine focussing mechanism
Lighting: 3 W LED (colour temperature 5000 K), adjustable
Integrated power supply unit 100...240 V, 50/60 Hz, 3W
Fuse: F2A/250V
Includes dust cover
Dimensions (H x W x D): 35 cm x 21 cm x 23 cm
Weight: 5.0 kg (monocular models); 5.5 kg (binocular mo-
dels)
8 WASTE DISPOSAL
The packaging consists predominately of environmentally
compatible materials because they can be recycled and
should be passed on for disposal by the local recycling service.
Should you no longer require this product,
do not dispose of it with the household re-
fuse.
Please return it to the address below for
proper waste disposal.
PHYWE Systeme GmbH & Co. KG
Service Department (Customer Service)
Robert-Bosch-Breite 10
37079 Göttingen / Germany
Phone [phone]
Fax [phone]
Mail [email protected], [email protected]